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[feature] : Tip broadening measurement #1018

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SylviaWhittle opened this issue Nov 20, 2024 · 1 comment
Open

[feature] : Tip broadening measurement #1018

SylviaWhittle opened this issue Nov 20, 2024 · 1 comment
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enhancement New feature or request

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@SylviaWhittle
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Is your feature request related to a problem?

During a group meeting, the experimentalists discussed adding a method for calculating the tip broadening of an AFM image. This would be very useful.

Describe the solution you would like.

The proposed method would be to:

  • Follow the DNA trace
  • At a configurable interval of nanometers, take a cross section of the DNA heights
  • Fit a gaussian to the height cross section
  • Calculate the width of the DNA based on the FWHM
  • Alternatively average all the height cross sections and take the FWHM from there
  • Assuming the DNA is as wide as it is tall, we can calculate the tip broadening by comparing width to height.
  • This also provides another method for calculating the width of the DNA.

This would need to be done after the ordered tracing stage, possibly after the splining stage?

Describe the alternatives you have considered.

None so far

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No response

@SylviaWhittle SylviaWhittle added the enhancement New feature or request label Nov 20, 2024
@ns-rse
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ns-rse commented Nov 20, 2024

Is this related to (or a duplicate of) #108 ?

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