Documentation needed for Fqtools splitpool

[kubu4]

I have the following script provided by BGI:

./iTools Fqtools splitpool
-InFq1 file1.fq.gz
-InFq2 file2.fq.gz
-Index index.lst
-Flag enzyme.txt
-MisMatch
-OutDir split/

What is the default setting for MisMatch?

Are there other options available?

[kubu4]

Also, what are the required file formatting for the -Flag input file and the -Index input file?

[hewm2008]

-Flag the file with enzyme specific sequence such EcoRI seq is : AATTC
-Index file with sample and barcodes [sample_ID sampleIndex ]

iTools Fqtools splitpool -InFq1 In_1.fq.gz -InFq2 In_1.fq.gz -Flag enzyme.seq -Index sample.index -OutDir OUTDir/ -MisMatch

cat enzyme.seq
AATTC

head -4 sample.index
S001 TGCGA
S002 GGTTGT
S003 ACGTGTT
S004 ACAGGGAA
@kubu4