biocathub.json

{"title": "Hotzymes-BmTA activity assay H2b", "description": "", "enzymes": [{"ecNumber": "2.6.1.1", "name": "Transaminase ", "organism": "Bacillus megaterium", "formulation": "Immobilosed on silicat-beads", "method": "Bradford ", "others": [{"key": "production organism", "value": "E.coli BL21"}, {"key": "enzyme immobilisation procedure", "value": "10.5281/zenodo.7352143"}], "reaction": {"educts": [{"name": "pyruvic acid", "role": "substrate", "concentration": "5", "unit": "mmol/L", "others": [], "smiles": "CC(=O)C(=O)[O-]"}, {"name": "1-phenylethylamine", "role": "substrate", "concentration": "2.5", "unit": "mmol/L", "others": [], "smiles": "CC(C1=CC=CC=C1)N"}], "products": [{"name": "acetophenone", "role": "product", "others": [], "concentration": "0", "unit": "mmoL/L", "smiles": "CC(=O)C1=CC=CC=C1"}, {"name": "alanine", "role": "product", "others": [], "concentration": "0", "unit": "mmol/L", "smiles": "CC(C(=O)O)N"}]}, "sequence": "MASRGSHHHHHHGASLTVQKINWEQVKEWDRKYLMRTFSTQNEYQPVPIESTEGDYLIMPDGTRLLDFFNQLYCVNLGQKNQKVNAAIKEALDRYGFVWDTYATDYKAKAAKIIIEDILGDEDWPGKVRFVSTGSEAVETALNIARLYTNRPLVVTREHDYHGWTGGAATVTRLRSYRSGLVGENSESFSAQIPGSSYNSAVLMAPSPNMFQDSDGNLLKDENGELLSVKYTRRMIENYGPEQVAAVITEVSQGAGSAMPPYEYIPQIRKMTKELGVLWINDEVLTGFGRTGKWFGYQHYGVQPDIITMGKGLSSSSLPAGAVLVSKEIAAFMDKHRWESVSTYAGHPVAMAAVCANLEVMMEENFVEQAKDSGEYIRSKLELLQEKHKSIGNFDGYGLLWIVDIVNAKTKTPYVKLDRNFTHGMNPNQIPTQIIMKKALEKGVLIGGVMPNTMRIGASLNVSRGDIDKAMDALDYALDYLESGEWQ", "concentration": "various"}], "vessel": {"type": "various", "others": []}, "condition": {"buffer": {"type": "HEPES", "concentration": "100", "unit": "mmol/L"}, "others": [{"key": "alternating magentic field (AMF)", "value": "602 kHz/27 mT"}], "temp": "25", "unit": "\u00b0C", "ph": "7.5"}, "experimentalData": {"measurements": [{"x_unit": "minutes", "x_name": "time", "y_unit": "mmol/L", "y_name": "concentration", "replicates": [{"x_value": 2, "y_values": [0.8076]}, {"x_value": 10, "y_values": [0.857]}, {"x_value": 20, "y_values": [0.8842]}, {"x_value": 30, "y_values": [0.9448]}, {"x_value": 60, "y_values": [1.0512]}, {"x_value": 90, "y_values": [1.1742]}, {"x_value": 120, "y_values": [1.1884]}], "notes": "Immobilised enzymes on silica beads, solubilised in 5mmol/L sbp (sample name =( H2b) with the magnetic field on", "plotStyle": "point"}, {"x_unit": "minutes", "x_name": "time", "y_unit": "mmol/L", "y_name": "concentration", "replicates": [{"x_value": 2, "y_values": [0.7121]}, {"x_value": 10, "y_values": [0.7993]}, {"x_value": 20, "y_values": [0.817]}, {"x_value": 30, "y_values": [0.8351]}, {"x_value": 60, "y_values": [0.8691]}, {"x_value": 90, "y_values": [1.0158]}, {"x_value": 120, "y_values": [1.0456]}], "notes": "Immobilised enzymes on silica beads, solubilised in 5mmol/L sbp (sample name =( H2b) with the magnetic field off", "plotStyle": "point"}]}, "user": {"lastName": "Macedo de Melo", "firstName": "Eduardo", "email": "edumacmelo@gmail.com", "institution": "Forschungszentrum J\u00fclich"}}