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Error in rgt-hint differential for my own data #267

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junichitaniguchi opened this issue Feb 8, 2024 · 0 comments
Open

Error in rgt-hint differential for my own data #267

junichitaniguchi opened this issue Feb 8, 2024 · 0 comments

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@junichitaniguchi
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Hello.

I have an issue during rgt-hint differential and it says:

Traceback (most recent call last):
File "/home/kthbecu/.local/bin/rgt-hint", line 8, in
sys.exit(main())
File "/home/kthbecu/.local/lib/python3.10/site-packages/rgt/HINT/Main.py", line 95, in main
args.func(args)
File "/home/kthbecu/.local/lib/python3.10/site-packages/rgt/HINT/DifferentialAnalysis.py", line 119, in diff_analysis_run
mpbs.read(mpbs_file)
File "/home/kthbecu/.local/lib/python3.10/site-packages/rgt/GenomicRegionSet.py", line 254, in read
io.read_to_grs(self, filename)
File "/home/kthbecu/.local/lib/python3.10/site-packages/rgt/GenomicRegionSet.py", line 52, in read_to_grs
with open(filename) as f:
FileNotFoundError: [Errno 2] No such file or directory: ''

This issue is specific to my own data which is ATAC-seq on human (hg38), mapped by bowtie2 and peak-called by MACS3.
rgt-hint differential on the tutorial data (DendriticCells) succeeded with no any error.
How can I solve this issue?

Thank you in advance.

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