diff --git a/cellacdc/dataPrep.py b/cellacdc/dataPrep.py
index 42b88055..29eb20e1 100755
--- a/cellacdc/dataPrep.py
+++ b/cellacdc/dataPrep.py
@@ -189,14 +189,6 @@ def gui_createActions(self):
 
         self.loadPosAction = QAction("Load different Position...", self)
         self.loadPosAction.setShortcut("Shift+P")
-        
-        toolTip = (
-            "Add crop ROI for multiple crops\n\n"
-            "Multiple crops will be saved as Position_1, Position_2 "
-            "as sub-folders in the current Position."
-        )
-        self.addCropRoiActon = QAction(QIcon(":add_crop_ROI.svg"), toolTip, self)
-        self.addCropRoiActon.setDisabled(True)
 
         toolTip = "Add ROI where to calculate background intensity"
         self.addBkrgRoiActon = QAction(QIcon(":bkgrRoi.svg"), toolTip, self)
@@ -260,7 +252,7 @@ def gui_createToolBars(self):
 
         # navigateToolbar.addAction(self.jumpBackwardAction)
         # navigateToolbar.addAction(self.jumpForwardAction)
-        navigateToolbar.addAction(self.addCropRoiActon)
+
         navigateToolbar.addAction(self.addBkrgRoiActon)
 
         navigateToolbar.addAction(self.ZbackAction)
@@ -284,7 +276,6 @@ def gui_connectActions(self):
         self.openRecentMenu.aboutToShow.connect(self.populateOpenRecent)
         self.exitAction.triggered.connect(self.close)
         self.showInExplorerAction.triggered.connect(self.showInExplorer)
-        self.addCropRoiActon.triggered.connect(self.addCropROI)
         self.addBkrgRoiActon.triggered.connect(self.addDefaultBkgrROI)
         self.cropAction.triggered.connect(self.crop_cb)
         self.cropZaction.toggled.connect(self.openCropZtool)
@@ -435,7 +426,7 @@ def updatePos(self):
         self.updateCropZtool()
         self.setImageNameText()
         self.update_img()
-        self.addAndConnectCropROIs()
+        self.updateROI()
         self.updateBkgrROIs()
         self.saveBkgrROIs(self.data[self.pos_i])
     
@@ -532,10 +523,7 @@ def update_img(self):
         self.img.setImage(img)
         self.zSliceScrollBar.setMaximum(posData.SizeZ-1)
 
-    def addAndConnectROI(self, roi):
-        pass
-    
-    def addAndConnectCropROIs(self):
+    def updateROI(self):
         if self.startAction.isEnabled() or self.onlySelectingZslice:
             return
 
@@ -1467,7 +1455,6 @@ def addROIs(self):
         self.ROIshapeComboBox.setCurrentText(items[-1])
 
         for posData in self.data:
-            posData.cropROIs = []
             if posData.dataPrep_ROIcoords is None:
                 cropROI = self.getDefaultROI()
             else:
@@ -1486,9 +1473,9 @@ def addROIs(self):
                 )
 
             self.setROIprops(cropROI)
-            posData.cropROIs.append(cropROI)
+            posData.cropROI = cropROI
 
-        self.addAndConnectCropROIs()
+        self.updateROI()
 
         try:
             self.ROIshapeComboBox.currentTextChanged.disconnect()
@@ -1499,7 +1486,6 @@ def addROIs(self):
         )
 
         self.addBkrgRoiActon.setDisabled(False)
-        self.addCropRoiActon.setDisabled(False)
 
         for posData in self.data:
             if not posData.bkgrROIs and not posData.bkgrDataExists:
@@ -1554,9 +1540,6 @@ def setBkgrROIprops(self, bkgrROI):
         # bkgrROI.sigRegionChanged.connect(self.bkgrROIMoving)
         # bkgrROI.sigRegionChangeFinished.connect(self.bkgrROImovingFinished)
 
-    def addCropROI(self):
-        cropROI = self.getDefaultROI()
-        self.setROIprops(cropROI)
 
     def addDefaultBkgrROI(self, checked=False):
         bkgrROI = self.getDefaultBkgrROI()
diff --git a/cellacdc/resources/icons/add_crop_ROI.svg b/cellacdc/resources/icons/add_crop_ROI.svg
deleted file mode 100644
index 078a3573..00000000
--- a/cellacdc/resources/icons/add_crop_ROI.svg
+++ /dev/null
@@ -1,65 +0,0 @@
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