Multiple contigs #1082
MonicaSteffi
started this conversation in
General
Replies: 0 comments
Sign up for free
to join this conversation on GitHub.
Already have an account?
Sign in to comment
-
Dear developer,
I used spades (with metaviral option) to do perform viral genome assembly. Spades gave us four different contigs. One of them is a big contig with 94315bp while the other three are smaller contigs around 2000-7000 bp. I assessed the quality of assembled contigs using checkV software. Larger contig had complete and high-quality and others have low-quality and genome-fragment.
I also blasted these four contigs against nucleotide blast NCBI. These four contigs has a similar hit with a particular (same) genome . All four contigs aligned at different positions with the query coverage between 70-88%
Do u think that these four contigs belong to one single genome? Is there any way to confirm this and if they belong to single genome, how do I combine them to make it as one big contig?
Thank you in advance.
Regards
Monica
Beta Was this translation helpful? Give feedback.
All reactions