diff --git a/lib/tinyusb b/lib/tinyusb
index 1fdf29075d4e6..d10b65ada4be7 160000
--- a/lib/tinyusb
+++ b/lib/tinyusb
@@ -1 +1 @@
-Subproject commit 1fdf29075d4e613eacfa881166015263797db0f6
+Subproject commit d10b65ada4be7d5754b3128e80a9b4db72bdb23f
diff --git a/ports/mimxrt/tusb_config.h b/ports/mimxrt/tusb_config.h
index 862fb6c52d2e5..607f36446f858 100644
--- a/ports/mimxrt/tusb_config.h
+++ b/ports/mimxrt/tusb_config.h
@@ -32,5 +32,6 @@
 #define CFG_TUD_CDC             (1)
 #define CFG_TUD_CDC_RX_BUFSIZE  (512)
 #define CFG_TUD_CDC_TX_BUFSIZE  (512)
+#define CFG_TUD_CDC_PERSISTENT_TX_BUFF (1)
 
 #endif // MICROPY_INCLUDED_MIMXRT_TUSB_CONFIG_H
diff --git a/shared/tinyusb/mp_usbd_cdc.c b/shared/tinyusb/mp_usbd_cdc.c
index 6d789ff5e81ca..cec06f307e55f 100644
--- a/shared/tinyusb/mp_usbd_cdc.c
+++ b/shared/tinyusb/mp_usbd_cdc.c
@@ -58,7 +58,9 @@ uintptr_t mp_usbd_cdc_poll_interfaces(uintptr_t poll_flags) {
     if ((poll_flags & MP_STREAM_POLL_RD) && ringbuf_peek(&stdin_ringbuf) != -1) {
         ret |= MP_STREAM_POLL_RD;
     }
-    if ((poll_flags & MP_STREAM_POLL_WR) && tud_cdc_connected() && tud_cdc_write_available() > 0) {
+    if ((poll_flags & MP_STREAM_POLL_WR) &&
+        (!tud_cdc_connected() || (tud_cdc_connected() && tud_cdc_write_available() > 0))) {
+        // Always allow write when not connected, fifo will retain latest.
         // When connected operate as blocking, only allow if space is available.
         ret |= MP_STREAM_POLL_WR;
     }
@@ -93,42 +95,60 @@ void tud_cdc_rx_cb(uint8_t itf) {
 
 mp_uint_t mp_usbd_cdc_tx_strn(const char *str, mp_uint_t len) {
     size_t i = 0;
-    if (tud_cdc_connected()) {
-        while (i < len) {
-            uint32_t n = len - i;
-            if (n > CFG_TUD_CDC_EP_BUFSIZE) {
-                n = CFG_TUD_CDC_EP_BUFSIZE;
-            }
-            int timeout = 0;
-            // Wait with a max of USC_CDC_TIMEOUT ms
-            while (n > tud_cdc_write_available() && timeout++ < MICROPY_HW_USB_CDC_TX_TIMEOUT) {
+    while (i < len) {
+        uint32_t n = len - i;
+        if (n > CFG_TUD_CDC_EP_BUFSIZE) {
+            n = CFG_TUD_CDC_EP_BUFSIZE;
+        }
+        int timeout = 0;
+        if (tud_cdc_connected()) {
+            // If cdc port is connected but the buffer is full,
+            // wait for up to USC_CDC_TIMEOUT ms
+            while (n > tud_cdc_write_available()
+                   && timeout++ < MICROPY_HW_USB_CDC_TX_TIMEOUT) {
                 mp_event_wait_ms(1);
 
                 // Explicitly run the USB stack as the scheduler may be locked (eg we
                 // are in an interrupt handler), while there is data pending.
                 mp_usbd_task();
             }
-            if (timeout >= MICROPY_HW_USB_CDC_TX_TIMEOUT) {
+            // Limit write to available space in tx buffer when connected.
+            n = MIN(n, tud_cdc_write_available());
+            if (n == 0) {
                 break;
             }
-            uint32_t n2 = tud_cdc_write(str + i, n);
-            tud_cdc_write_flush();
-            i += n2;
         }
+        // When not connected we always write to usb fifo, ensuring it has latest data.
+        uint32_t n2 = tud_cdc_write(str + i, n);
+        tud_cdc_write_flush();
+        i += n2;
     }
     return i;
 }
 
+static int8_t cdc_connected_flush_delay = 0;
+
+void tud_sof_cb(uint32_t frame_count) {
+    if (--cdc_connected_flush_delay < 0) {
+        // Finished on-connection delay, disable SOF interrupt again.
+        // Finished on-connection delay, disable SOF interrupt again.
+        tud_sof_cb_enable(false);
+        tud_cdc_write_flush();
+    }
+}
+
 #endif
 
-#if MICROPY_HW_USB_CDC_1200BPS_TOUCH && MICROPY_HW_ENABLE_USBDEV
+#if MICROPY_HW_ENABLE_USBDEV && (MICROPY_HW_USB_CDC_1200BPS_TOUCH || MICROPY_HW_USB_CDC)
 
+#if MICROPY_HW_USB_CDC_1200BPS_TOUCH
 static mp_sched_node_t mp_bootloader_sched_node;
 
 static void usbd_cdc_run_bootloader_task(mp_sched_node_t *node) {
     mp_hal_delay_ms(250);
     machine_bootloader(0, NULL);
 }
+#endif
 
 void
 #if MICROPY_HW_USB_EXTERNAL_TINYUSB
@@ -137,6 +157,16 @@ mp_usbd_line_state_cb
 tud_cdc_line_state_cb
 #endif
     (uint8_t itf, bool dtr, bool rts) {
+    #if MICROPY_HW_USB_CDC
+    if (dtr) {
+        // A host application has started to open the cdc serial port.
+        // Wait a few ms for host to be ready then send tx buffer.
+        // High speed connection SOF fires at 125us, full speed at 1ms.
+        cdc_connected_flush_delay = (tud_speed_get() == TUSB_SPEED_HIGH) ? 128 : 16;
+        tud_sof_cb_enable(true);
+    }
+    #endif
+    #if MICROPY_HW_USB_CDC_1200BPS_TOUCH
     if (dtr == false && rts == false) {
         // Device is disconnected.
         cdc_line_coding_t line_coding;
@@ -146,6 +176,7 @@ tud_cdc_line_state_cb
             mp_sched_schedule_node(&mp_bootloader_sched_node, usbd_cdc_run_bootloader_task);
         }
     }
+    #endif
 }
 
 #endif
diff --git a/shared/tinyusb/tusb_config.h b/shared/tinyusb/tusb_config.h
index ab47321afdc04..1f8f5e5f6dc56 100644
--- a/shared/tinyusb/tusb_config.h
+++ b/shared/tinyusb/tusb_config.h
@@ -79,6 +79,7 @@
 #if CFG_TUD_CDC
 #define CFG_TUD_CDC_RX_BUFSIZE  ((CFG_TUD_MAX_SPEED == OPT_MODE_HIGH_SPEED) ? 512 : 256)
 #define CFG_TUD_CDC_TX_BUFSIZE  ((CFG_TUD_MAX_SPEED == OPT_MODE_HIGH_SPEED) ? 512 : 256)
+#define CFG_TUD_CDC_PERSISTENT_TX_BUFF (1)
 #endif
 
 // MSC Configuration