Implement Gibson Assembly

[carreter]

Blocked by #367

[carreter]

@TimothyStiles can you elaborate on what exactly it is we need here and triage the issue here + in the roadmap?

[Koeng101]

I think I can explain a little bit.

Gibson Assembly is an extremely popular DNA assembly method. It's used by pretty much everyone who isn't doing high-throughput work (where GoldenGate usually works better, which we do have).

Fundamentally, pretty much all DNA assembly methods use some form of overlapping sticky DNA ends which are then ligated together. In restriction enzyme cloning, these overhangs are created with enzymes that cut the DNA. In Gibson, you take blunted fragments and chew back about 20bp-40bp, which is then used as the overlap region (an idea originally adapted from yeast, which do this naturally).

So, with just a few additions, we could probably adapt our clone library to do Gibson. However, I think the clone library out of all our packages (other than parsers) could really use a revamp. It's kinda convoluted right now, and I think it could be simplified by quite a bit.

Gibson assembly I think is important enough that we should have it on the roadmap.

[github-actions]

Status: Ready to merge ✔️

Issues blocking this PR:

• Refactor clone package #367 ✔️

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[github-actions]

This issue has had no activity in the past 2 months. Marking as stale.

[github-actions]

This issue has had no activity in the past 2 months. Marking as stale.