Regarding the necessity of normal.bam file

[ghost]

I am currently working on a tumor bulk RNA-seq data set in order to identify somatic mutations. I have the BAM file for the tumor data set but I do not have the BAM file for a normal sample. Is there any way to use NeuSomatic without a normal BAM file?

[amit21AIT]

Ya same issue .

[msahraeian]

Thanks for your interest in NeuSomatic.
Currently, NeuSomatic can only support tumor-normal somatic mutation detection. We plan to extend it to tumor-only mode in near future.

[amit21AIT]

Can you suggest any other tool or technique for somatic mutation detection using tumor - only mode because i don't have the normal.bam file

[msahraeian]

@amit21AIT I guess methods like MuTect2 and VarDict can perform tumor-only calling, although they may have difficulty in filtering some FPs and germline calls without normal match.

[ldcato]

Just wondering if there was an update from the developers on when an extension to tumor-only mode may be available?