From fc592f24e946220d308350b62af8474d7d4889e4 Mon Sep 17 00:00:00 2001 From: Sahraeian Date: Fri, 26 Jul 2019 14:40:37 -0700 Subject: [PATCH 01/15] added post_filter_dup --- neusomatic/python/generate_dataset.py | 11 ++++++++-- neusomatic/python/long_read_indelrealign.py | 22 ++++++++++++++----- neusomatic/python/postprocess.py | 12 +++++++++-- neusomatic/python/resolve_scores.py | 2 +- neusomatic/python/resolve_variants.py | 24 +++++++++++++-------- 5 files changed, 52 insertions(+), 19 deletions(-) diff --git a/neusomatic/python/generate_dataset.py b/neusomatic/python/generate_dataset.py index 4862a66..10422e8 100755 --- a/neusomatic/python/generate_dataset.py +++ b/neusomatic/python/generate_dataset.py @@ -873,6 +873,7 @@ def find_records(input_record): records = [] i = 0 anns = {} + fasta_file = pysam.Fastafile(ref_file) if ensemble_bed: for record in not_in_ensemble_bed: chrom, pos, ref, alt = [str(record[0]), int( @@ -987,8 +988,15 @@ def find_records(input_record): if line[0] == "#": continue record = line.strip().split() + pos = int(record[1]) + if len(record[3]) != len(record[4]) and min(len(record[3]),len(record[4]))>0 and record[3][0] != record[4][0]: + if pos > 1: + l_base = fasta_file.fetch(record[0], pos - 2, pos - 1).upper() + record[3] = l_base + record[3] + record[4] = l_base + record[4] + pos -= 1 truth_records.append( - [record[0], int(record[1]), record[3], record[4], str(i)]) + [record[0], pos, record[3], record[4], str(i)]) i += 1 truth_bed = pybedtools.BedTool(map(lambda x: pybedtools.Interval( @@ -1011,7 +1019,6 @@ def find_records(input_record): record_center = {} chroms_order = get_chromosomes_order(reference=ref_file) - fasta_file = pysam.Fastafile(ref_file) good_records = {"INS": [], "DEL": [], "SNP": []} vtype = {} diff --git a/neusomatic/python/long_read_indelrealign.py b/neusomatic/python/long_read_indelrealign.py index 0bb8e09..2fc4907 100755 --- a/neusomatic/python/long_read_indelrealign.py +++ b/neusomatic/python/long_read_indelrealign.py @@ -279,7 +279,7 @@ def cigartuple_to_string(cigartuples): return "".join(map(lambda x: "%d%s" % (x[1], _CIGAR_OPS[x[0]]), cigartuples)) -def prepare_fasta(work, region, input_bam, ref_fasta_file, include_ref, split_i): +def prepare_fasta(work, region, input_bam, ref_fasta_file, include_ref, split_i, filter_duplicate): logger = logging.getLogger(prepare_fasta.__name__) in_fasta_file = os.path.join( work, region.__str__() + "_split_{}".format(split_i) + "_0.fasta") @@ -296,6 +296,8 @@ def prepare_fasta(work, region, input_bam, ref_fasta_file, include_ref, split_i) cnt = 1 with pysam.Samfile(input_bam, "rb") as samfile: for record in samfile.fetch(region.chrom, region.start, region.end + 1): + if filter_duplicate and record.is_duplicate: + continue if record.is_supplementary and "SA" in dict(record.tags): sas = dict(record.tags)["SA"].split(";") sas = list(filter(None, sas)) @@ -371,11 +373,13 @@ def prepare_fasta(work, region, input_bam, ref_fasta_file, include_ref, split_i) def split_bam_to_chunks(work, region, input_bam, chunk_size=200, - chunk_scale=1.5): + chunk_scale=1.5, filter_duplicate=False): logger = logging.getLogger(split_bam_to_chunks.__name__) records = [] with pysam.Samfile(input_bam, "rb") as samfile: for record in samfile.fetch(region.chrom, region.start, region.end + 1): + if filter_duplicate and record.is_duplicate: + continue if record.is_supplementary and "SA" in dict(record.tags): sas = dict(record.tags)["SA"].split(";") sas = list(filter(None, sas)) @@ -906,6 +910,7 @@ def run_realignment(input_record): work, ref_fasta_file, target_region, pad, chunk_size, chunk_scale, \ snp_min_af, del_min_af, ins_min_af, len_chr, input_bam, \ match_score, mismatch_penalty, gap_open_penalty, gap_ext_penalty, \ + filter_duplicate, \ msa_binary, get_var = input_record thread_logger = logging.getLogger( @@ -922,7 +927,7 @@ def run_realignment(input_record): variant = [] all_entries = [] input_bam_splits, lens_splits = split_bam_to_chunks( - work, region, input_bam, chunk_size, chunk_scale) + work, region, input_bam, chunk_size, chunk_scale, filter_duplicate) new_seqs = [] new_ref_seq = "" skipped = 0 @@ -933,7 +938,7 @@ def run_realignment(input_record): afss = [] for i, i_bam in enumerate(input_bam_splits): in_fasta_file, info_file = prepare_fasta( - work, region, i_bam, ref_fasta_file, True, i) + work, region, i_bam, ref_fasta_file, True, i, filter_duplicate) if do_realign(region, info_file): out_fasta_file_0 = run_msa( in_fasta_file, match_score, mismatch_penalty, gap_open_penalty, @@ -1168,6 +1173,7 @@ def long_read_indelrealign(work, input_bam, output_bam, output_vcf, region_bed_f ref_fasta_file, num_threads, pad, chunk_size, chunk_scale, snp_min_af, del_min_af, ins_min_af, match_score, mismatch_penalty, gap_open_penalty, gap_ext_penalty, + filter_duplicate, msa_binary): logger = logging.getLogger(long_read_indelrealign.__name__) @@ -1217,6 +1223,7 @@ def long_read_indelrealign(work, input_bam, output_bam, output_vcf, region_bed_f chunk_scale, snp_min_af, del_min_af, ins_min_af, chrom_lengths[target_region[0]], input_bam, match_score, mismatch_penalty, gap_open_penalty, gap_ext_penalty, + filter_duplicate, msa_binary, get_var)) shuffle(map_args) @@ -1321,6 +1328,9 @@ def long_read_indelrealign(work, input_bam, output_bam, output_vcf, region_bed_f help='penalty for opening a gap', default=8) parser.add_argument('--gap_ext_penalty', type=int, help='penalty for extending a gap', default=6) + parser.add_argument('--filter_duplicate', + help='filter duplicate reads in analysis', + action="store_true") parser.add_argument('--msa_binary', type=str, help='MSA binary', default="../bin/msa") args = parser.parse_args() @@ -1333,7 +1343,9 @@ def long_read_indelrealign(work, input_bam, output_bam, output_vcf, region_bed_f args.chunk_scale, args.snp_min_af, args.del_min_af, args.ins_min_af, args.match_score, args.mismatch_penalty, args.gap_open_penalty, - args.gap_ext_penalty, args.msa_binary) + args.gap_ext_penalty, + args.filter_duplicate, + args.msa_binary) except Exception as e: logger.error(traceback.format_exc()) logger.error("Aborting!") diff --git a/neusomatic/python/postprocess.py b/neusomatic/python/postprocess.py index 5085f65..4629d3d 100755 --- a/neusomatic/python/postprocess.py +++ b/neusomatic/python/postprocess.py @@ -155,6 +155,7 @@ def postprocess(work, reference, pred_vcf_file, output_vcf, candidates_vcf, ense lr_snp_min_af, lr_ins_min_af, lr_del_min_af, lr_match_score, lr_mismatch_penalty, lr_gap_open_penalty, lr_gap_ext_penalty, pass_threshold, lowqual_threshold, + filter_duplicate, msa_binary, num_threads): logger = logging.getLogger(postprocess.__name__) @@ -187,7 +188,8 @@ def postprocess(work, reference, pred_vcf_file, output_vcf, candidates_vcf, ense logger.info("Resolve targets") if not long_read: resolve_variants(tumor_bam, resolved_vcf, - reference, target_vcf, target_bed, num_threads) + reference, target_vcf, target_bed, filter_duplicate, + num_threads) else: work_lr_indel_realign = os.path.join(work, "work_lr_indel_realign") if os.path.exists(work_lr_indel_realign): @@ -200,7 +202,9 @@ def postprocess(work, reference, pred_vcf_file, output_vcf, candidates_vcf, ense lr_chunk_size, lr_chunk_scale, lr_snp_min_af, lr_del_min_af, lr_ins_min_af, lr_match_score, lr_mismatch_penalty, lr_gap_open_penalty, - lr_gap_ext_penalty, msa_binary) + lr_gap_ext_penalty, + filter_duplicate, + msa_binary) resolve_scores(tumor_bam, ra_resolved_vcf, target_vcf, resolved_vcf) all_no_resolve = concatenate_files( @@ -274,6 +278,9 @@ def postprocess(work, reference, pred_vcf_file, output_vcf, candidates_vcf, ense parser.add_argument('--lowqual_threshold', type=float, help='SCORE for LowQual (PASS for lowqual_threshold <= score < pass_threshold)', default=0.4) + parser.add_argument('--filter_duplicate', + help='filter duplicate reads in analysis', + action="store_true") parser.add_argument('--msa_binary', type=str, help='MSA binary', default="../bin/msa") parser.add_argument('--num_threads', type=int, @@ -293,6 +300,7 @@ def postprocess(work, reference, pred_vcf_file, output_vcf, candidates_vcf, ense args.lr_match_score, args.lr_mismatch_penalty, args.lr_gap_open_penalty, args.lr_gap_ext_penalty, args.pass_threshold, args.lowqual_threshold, + args.filter_duplicate, args.msa_binary, args.num_threads) except Exception as e: diff --git a/neusomatic/python/resolve_scores.py b/neusomatic/python/resolve_scores.py index a2897c6..fd4f5f6 100755 --- a/neusomatic/python/resolve_scores.py +++ b/neusomatic/python/resolve_scores.py @@ -24,7 +24,7 @@ def resolve_scores(input_bam, ra_vcf, target_vcf, output_vcf): final_intervals = [] for interval in ra_out.window(ra_target, w=5, v=True): - interval[5] = "0.5" + interval[5] = str(np.round(-10*np.log10(0.25),4)) final_intervals.append(interval) intervals_dict = {} diff --git a/neusomatic/python/resolve_variants.py b/neusomatic/python/resolve_variants.py index 39181d8..7c58331 100755 --- a/neusomatic/python/resolve_variants.py +++ b/neusomatic/python/resolve_variants.py @@ -71,7 +71,7 @@ def extract_ins(record): def find_resolved_variants(input_record): - chrom, start, end, variants, input_bam, reference = input_record + chrom, start, end, variants, input_bam, filter_duplicate, reference = input_record thread_logger = logging.getLogger( "{} ({})".format(find_resolved_variants.__name__, multiprocessing.current_process().name)) try: @@ -83,7 +83,7 @@ def find_resolved_variants(input_record): scores = list(map(lambda x: x[5], variants)) if len(set(vartypes)) > 1: out_variants.extend( - list(map(lambda x: [x[0], int(x[1]), x[3], x[4], x[10], x[5]], variants))) + list(map(lambda x: [x[0], int(x[1]), x[3], x[4], x[9].split(":")[0], x[5]], variants))) else: vartype = vartypes[0] score = max(scores) @@ -92,8 +92,9 @@ def find_resolved_variants(input_record): dels = [] with pysam.AlignmentFile(input_bam) as samfile: for record in samfile.fetch(chrom, start, end): - if record.cigarstring and "D" in record.cigarstring: - dels.extend(extract_del(record)) + if not record.is_duplicate or not filter_duplicate: + if record.cigarstring and "D" in record.cigarstring: + dels.extend(extract_del(record)) dels = list(filter(lambda x: ( start <= x[1] <= end) or start <= x[2] <= end, dels)) if dels: @@ -119,8 +120,9 @@ def find_resolved_variants(input_record): inss = [] with pysam.AlignmentFile(input_bam) as samfile: for record in samfile.fetch(chrom, start, end): - if record.cigarstring and "I" in record.cigarstring: - inss.extend(extract_ins(record)) + if not record.is_duplicate or not filter_duplicate: + if record.cigarstring and "I" in record.cigarstring: + inss.extend(extract_ins(record)) inss = list(filter(lambda x: ( start <= x[1] <= end) or start <= x[2] <= end, inss)) if inss: @@ -150,7 +152,7 @@ def find_resolved_variants(input_record): def resolve_variants(input_bam, resolved_vcf, reference, target_vcf_file, - target_bed_file, num_threads): + target_bed_file, filter_duplicate, num_threads): logger = logging.getLogger(resolve_variants.__name__) logger.info("-------Resolve variants (e.g. exact INDEL sequences)-------") @@ -177,7 +179,7 @@ def resolve_variants(input_bam, resolved_vcf, reference, target_vcf_file, chrom, start, end, id_ = tb[0:4] id_ = int(id_) map_args.append([chrom, start, end, variants[id_], - input_bam, reference]) + input_bam, filter_duplicate, reference]) pool = multiprocessing.Pool(num_threads) try: @@ -230,6 +232,9 @@ def resolve_variants(input_bam, resolved_vcf, reference, target_vcf_file, help='resolve target bed', required=True) parser.add_argument('--reference', type=str, help='reference fasta filename', required=True) + parser.add_argument('--filter_duplicate', + help='filter duplicate reads in analysis', + action="store_true") parser.add_argument('--num_threads', type=int, help='number of threads', default=1) args = parser.parse_args() @@ -237,7 +242,8 @@ def resolve_variants(input_bam, resolved_vcf, reference, target_vcf_file, try: resolve_variants(args.input_bam, args.resolved_vcf, args.reference, args.target_vcf, - args.target_bed, args.num_threads) + args.target_bed, args.filter_duplicate, + args.num_threads) except Exception as e: logger.error(traceback.format_exc()) logger.error("Aborting!") From e554bf281a4ffd954aa3a4760f852f034ac50661 Mon Sep 17 00:00:00 2001 From: Sahraeian Date: Tue, 13 Aug 2019 13:46:06 -0700 Subject: [PATCH 02/15] fix for long-read high-cov indelrealignment --- neusomatic/python/long_read_indelrealign.py | 119 +++++++++++++------- neusomatic/python/postprocess.py | 27 ++++- 2 files changed, 97 insertions(+), 49 deletions(-) diff --git a/neusomatic/python/long_read_indelrealign.py b/neusomatic/python/long_read_indelrealign.py index 0bb8e09..d419cc7 100755 --- a/neusomatic/python/long_read_indelrealign.py +++ b/neusomatic/python/long_read_indelrealign.py @@ -279,7 +279,7 @@ def cigartuple_to_string(cigartuples): return "".join(map(lambda x: "%d%s" % (x[1], _CIGAR_OPS[x[0]]), cigartuples)) -def prepare_fasta(work, region, input_bam, ref_fasta_file, include_ref, split_i): +def prepare_fasta(work, region, input_bam, ref_fasta_file, include_ref, split_i, ds): logger = logging.getLogger(prepare_fasta.__name__) in_fasta_file = os.path.join( work, region.__str__() + "_split_{}".format(split_i) + "_0.fasta") @@ -308,6 +308,8 @@ def prepare_fasta(work, region, input_bam, ref_fasta_file, include_ref, split_i) full_length=True))))) if not record.cigartuples: continue + if np.random.rand() > ds: + continue sc_start = (record.cigartuples[0][0] == CIGAR_SOFTCLIP) * record.cigartuples[0][1] sc_end = (record.cigartuples[-1][0] == @@ -371,7 +373,7 @@ def prepare_fasta(work, region, input_bam, ref_fasta_file, include_ref, split_i) def split_bam_to_chunks(work, region, input_bam, chunk_size=200, - chunk_scale=1.5): + chunk_scale=1.5, do_split=False): logger = logging.getLogger(split_bam_to_chunks.__name__) records = [] with pysam.Samfile(input_bam, "rb") as samfile: @@ -409,11 +411,13 @@ def split_bam_to_chunks(work, region, input_bam, chunk_size=200, if len(records) < chunk_size * chunk_scale: bams = [input_bam] lens = [len(records)] - else: + ds = [1] + elif do_split: n_splits = int(max(6, len(records) // chunk_size)) new_chunk_size = len(records) // n_splits bams = [] lens = [] + ds = [] n_split = (len(records) // new_chunk_size) + 1 if 0 < (len(records) - ((n_split - 1) * new_chunk_size) + new_chunk_size) \ < new_chunk_size * chunk_scale: @@ -437,7 +441,12 @@ def split_bam_to_chunks(work, region, input_bam, chunk_size=200, bams.append(split_input_bam) lens.append(i_end - i_start + 1) - return bams, lens + ds.append(1) + else: + bams = [input_bam] + lens = [chunk_size * chunk_scale] + ds = [chunk_size * chunk_scale / float(len(records))] + return bams, lens, ds def read_info(info_file): @@ -809,7 +818,7 @@ def run_msa(in_fasta_file, match_score, mismatch_penalty, gap_open_penalty, gap_ return out_fasta_file -def do_realign(region, info_file, thr_realign=0.0135, max_N=1000): +def do_realign(region, info_file, max_realign_dp, thr_realign=0.0135): logger = logging.getLogger(do_realign.__name__) sum_nm_snp = 0 sum_nm_indel = 0 @@ -821,7 +830,7 @@ def do_realign(region, info_file, thr_realign=0.0135, max_N=1000): sum_nm_indel += int(x[-1]) c += 1 eps = 0.0001 - if (c < max_N) and ( + if (c < max_realign_dp) and ( (sum_nm_snp + sum_nm_indel ) / float(c + eps) / float(region.span() + eps) > thr_realign): @@ -906,14 +915,15 @@ def run_realignment(input_record): work, ref_fasta_file, target_region, pad, chunk_size, chunk_scale, \ snp_min_af, del_min_af, ins_min_af, len_chr, input_bam, \ match_score, mismatch_penalty, gap_open_penalty, gap_ext_penalty, \ - msa_binary, get_var = input_record + max_realign_dp, \ + msa_binary, get_var, do_split = input_record thread_logger = logging.getLogger( "{} ({})".format(run_realignment.__name__, multiprocessing.current_process().name)) try: region = Region(target_region, pad, len_chr) - + not_realigned_region = None original_tempdir = pybedtools.get_tempdir() pybedtmp = os.path.join(work, "pybedtmp_{}".format(region.__str__())) if not os.path.exists(pybedtmp): @@ -921,8 +931,8 @@ def run_realignment(input_record): pybedtools.set_tempdir(pybedtmp) variant = [] all_entries = [] - input_bam_splits, lens_splits = split_bam_to_chunks( - work, region, input_bam, chunk_size, chunk_scale) + input_bam_splits, lens_splits, ds_splits = split_bam_to_chunks( + work, region, input_bam, chunk_size, chunk_scale, do_split or not get_var) new_seqs = [] new_ref_seq = "" skipped = 0 @@ -933,8 +943,8 @@ def run_realignment(input_record): afss = [] for i, i_bam in enumerate(input_bam_splits): in_fasta_file, info_file = prepare_fasta( - work, region, i_bam, ref_fasta_file, True, i) - if do_realign(region, info_file): + work, region, i_bam, ref_fasta_file, True, i, ds_splits[i]) + if do_realign(region, info_file, max_realign_dp): out_fasta_file_0 = run_msa( in_fasta_file, match_score, mismatch_penalty, gap_open_penalty, gap_ext_penalty, msa_binary) @@ -952,37 +962,41 @@ def run_realignment(input_record): all_entries.extend(entries) else: skipped += 1 - if get_var and new_seqs: - for i in range(skipped): + if get_var: + if new_seqs: + for i in range(skipped): + new_seqs = [new_ref_seq] + new_seqs new_seqs = [new_ref_seq] + new_seqs - new_seqs = [new_ref_seq] + new_seqs - consensus_fasta = os.path.join( - work, region.__str__() + "_consensus.fasta") - with open(consensus_fasta, "w") as output_handle: - for i, seq in enumerate(new_seqs): - record = SeqRecord( - Seq(seq, DNAAlphabet.letters), id=str(i), description="") - SeqIO.write(record, output_handle, "fasta") - consensus_fasta_aligned = run_msa( - consensus_fasta, match_score, mismatch_penalty, gap_open_penalty, - gap_ext_penalty, msa_binary) - ref_seq, alt_seq, afs = find_var( - consensus_fasta_aligned, snp_min_af, del_min_af, ins_min_af, 1) - if ref_seq != alt_seq: - ref, alt, pos = TrimREFALT( - ref_seq, alt_seq, int(region.start) + 1) - a = int(np.ceil(np.max(afs) * len(afss))) - af = sum(sorted(map(lambda x: - np.max(x) if x.shape[0] > 0 else 0, - afss))[-a:]) / float(len(afss)) - dp = sum(lens_splits) - ao = int(af * dp) - ro = dp - ao - variant = [region.chrom, pos, ref, alt, dp, ro, ao] + consensus_fasta = os.path.join( + work, region.__str__() + "_consensus.fasta") + with open(consensus_fasta, "w") as output_handle: + for i, seq in enumerate(new_seqs): + record = SeqRecord( + Seq(seq, DNAAlphabet.letters), id=str(i), description="") + SeqIO.write(record, output_handle, "fasta") + consensus_fasta_aligned = run_msa( + consensus_fasta, match_score, mismatch_penalty, gap_open_penalty, + gap_ext_penalty, msa_binary) + ref_seq, alt_seq, afs = find_var( + consensus_fasta_aligned, snp_min_af, del_min_af, ins_min_af, 1) + if ref_seq != alt_seq: + ref, alt, pos = TrimREFALT( + ref_seq, alt_seq, int(region.start) + 1) + a = int(np.ceil(np.max(afs) * len(afss))) + af = sum(sorted(map(lambda x: + np.max(x) if x.shape[0] > 0 else 0, + afss))[-a:]) / float(len(afss)) + dp = sum(lens_splits) + ao = int(af * dp) + ro = dp - ao + variant = [region.chrom, pos, ref, alt, dp, ro, ao] + else: + if skipped>0 : + not_realigned_region = target_region shutil.rmtree(pybedtmp) pybedtools.set_tempdir(original_tempdir) - return all_entries, variant + return all_entries, variant, not_realigned_region except Exception as ex: thread_logger.error(traceback.format_exc()) thread_logger.error(ex) @@ -1164,10 +1178,13 @@ def extend_regions_repeat(region_bed_file, extended_region_bed_file, ref_fasta_f pybedtools.BedTool(intervals).sort().saveas(extended_region_bed_file) -def long_read_indelrealign(work, input_bam, output_bam, output_vcf, region_bed_file, +def long_read_indelrealign(work, input_bam, output_bam, output_vcf, output_not_realigned_bed, + region_bed_file, ref_fasta_file, num_threads, pad, chunk_size, chunk_scale, snp_min_af, del_min_af, ins_min_af, match_score, mismatch_penalty, gap_open_penalty, gap_ext_penalty, + max_realign_dp, + do_split, msa_binary): logger = logging.getLogger(long_read_indelrealign.__name__) @@ -1217,7 +1234,8 @@ def long_read_indelrealign(work, input_bam, output_bam, output_vcf, region_bed_f chunk_scale, snp_min_af, del_min_af, ins_min_af, chrom_lengths[target_region[0]], input_bam, match_score, mismatch_penalty, gap_open_penalty, gap_ext_penalty, - msa_binary, get_var)) + max_realign_dp, + msa_binary, get_var, do_split)) shuffle(map_args) try: @@ -1237,6 +1255,8 @@ def long_read_indelrealign(work, input_bam, output_bam, output_vcf, region_bed_f realign_variants = list(map(lambda x: x[1], realign_output)) realign_variants = list(filter(None, realign_variants)) + not_realigned_regions = list(map(lambda x: x[2], realign_output)) + not_realigned_regions = list(filter(None, not_realigned_regions)) if get_var: with open(output_vcf, "w") as o_f: @@ -1252,6 +1272,9 @@ def long_read_indelrealign(work, input_bam, output_bam, output_vcf, region_bed_f "GT:DP:RO:AO", "0/1:{}:{}:{}".format( dp, ro, ao), ]) o_f.write(line + "\n") + with open(output_not_realigned_bed, "w") as o_f: + for x in not_realigned_regions: + o_f.write("\t".join(map(str, x)) + "\n") original_tempdir = pybedtools.get_tempdir() pybedtmp = os.path.join(work, "pybedtmp") @@ -1291,6 +1314,8 @@ def long_read_indelrealign(work, input_bam, output_bam, output_vcf, region_bed_f parser.add_argument('--input_bam', type=str, help='input bam') parser.add_argument('--output_vcf', type=str, help='output_vcf (needed for variant prediction)', default=None) + parser.add_argument('--output_not_realigned_bed', type=str, + help='output_not_realigned_bed', required=True) parser.add_argument('--output_bam', type=str, help='output_bam (needed for getting the realigned bam)', default=None) parser.add_argument('--region_bed', type=str, @@ -1321,6 +1346,11 @@ def long_read_indelrealign(work, input_bam, output_bam, output_vcf, region_bed_f help='penalty for opening a gap', default=8) parser.add_argument('--gap_ext_penalty', type=int, help='penalty for extending a gap', default=6) + parser.add_argument('--max_realign_dp', type=int, + help='max coverage for realign region', default=1000) + parser.add_argument('--do_split', + help='Split bam for high coverage regions (in variant-calling mode).', + action="store_true") parser.add_argument('--msa_binary', type=str, help='MSA binary', default="../bin/msa") args = parser.parse_args() @@ -1328,12 +1358,15 @@ def long_read_indelrealign(work, input_bam, output_bam, output_vcf, region_bed_f try: processor = long_read_indelrealign(args.work, args.input_bam, args.output_bam, - args.output_vcf, args.region_bed, args.reference, + args.output_vcf, args.output_not_realigned_bed, + args.region_bed, args.reference, args.num_threads, args.pad, args.chunk_size, args.chunk_scale, args.snp_min_af, args.del_min_af, args.ins_min_af, args.match_score, args.mismatch_penalty, args.gap_open_penalty, - args.gap_ext_penalty, args.msa_binary) + args.gap_ext_penalty, args.max_realign_dp, + args.do_split, + args.msa_binary) except Exception as e: logger.error(traceback.format_exc()) logger.error("Aborting!") diff --git a/neusomatic/python/postprocess.py b/neusomatic/python/postprocess.py index 5085f65..802bcad 100755 --- a/neusomatic/python/postprocess.py +++ b/neusomatic/python/postprocess.py @@ -153,7 +153,7 @@ def postprocess(work, reference, pred_vcf_file, output_vcf, candidates_vcf, ense postprocess_max_dist, long_read, lr_pad, lr_chunk_size, lr_chunk_scale, lr_snp_min_af, lr_ins_min_af, lr_del_min_af, lr_match_score, lr_mismatch_penalty, - lr_gap_open_penalty, lr_gap_ext_penalty, + lr_gap_open_penalty, lr_gap_ext_penalty, lr_max_realign_dp, lr_do_split, pass_threshold, lowqual_threshold, msa_binary, num_threads): logger = logging.getLogger(postprocess.__name__) @@ -188,6 +188,8 @@ def postprocess(work, reference, pred_vcf_file, output_vcf, candidates_vcf, ense if not long_read: resolve_variants(tumor_bam, resolved_vcf, reference, target_vcf, target_bed, num_threads) + all_no_resolve = concatenate_files( + [no_resolve, ensembled_preds], os.path.join(work, "no_resolve.vcf")) else: work_lr_indel_realign = os.path.join(work, "work_lr_indel_realign") if os.path.exists(work_lr_indel_realign): @@ -195,16 +197,22 @@ def postprocess(work, reference, pred_vcf_file, output_vcf, candidates_vcf, ense os.mkdir(work_lr_indel_realign) ra_resolved_vcf = os.path.join( work, "candidates_preds.ra_resolved.vcf") - long_read_indelrealign(work_lr_indel_realign, tumor_bam, None, ra_resolved_vcf, target_bed, + not_resolved_bed = os.path.join(work, "candidates_preds.not_ra_resolved.bed") + long_read_indelrealign(work_lr_indel_realign, tumor_bam, None, ra_resolved_vcf, + not_resolved_bed, target_bed, reference, num_threads, lr_pad, lr_chunk_size, lr_chunk_scale, lr_snp_min_af, lr_del_min_af, lr_ins_min_af, lr_match_score, lr_mismatch_penalty, lr_gap_open_penalty, - lr_gap_ext_penalty, msa_binary) + lr_gap_ext_penalty, lr_max_realign_dp, lr_do_split, + msa_binary) resolve_scores(tumor_bam, ra_resolved_vcf, target_vcf, resolved_vcf) - all_no_resolve = concatenate_files( - [no_resolve, ensembled_preds], os.path.join(work, "no_resolve.vcf")) + not_resolved_vcf = os.path.join(work, "candidates_preds.not_ra_resolved.vcf") + pybedtools.BedTool(target_vcf).intersect(not_resolved_bed, u=True).saveas(not_resolved_vcf) + + all_no_resolve = concatenate_files( + [no_resolve, ensembled_preds, not_resolved_vcf], os.path.join(work, "no_resolve.vcf")) logger.info("Merge vcfs") merged_vcf = os.path.join(work, "merged_preds.vcf") @@ -269,6 +277,11 @@ def postprocess(work, reference, pred_vcf_file, output_vcf, candidates_vcf, ense help='long_read indel realign: penalty for opening a gap', default=8) parser.add_argument('--lr_gap_ext_penalty', type=int, help='long_read indel realign: penalty for extending a gap', default=6) + parser.add_argument('--lr_max_realign_dp', type=int, + help='long read max coverage for realign region', default=1000) + parser.add_argument('--lr_do_split', + help='long read split bam for high coverage regions (in variant-calling mode).', + action="store_true") parser.add_argument('--pass_threshold', type=float, help='SCORE for PASS (PASS for score => pass_threshold)', default=0.7) parser.add_argument('--lowqual_threshold', type=float, @@ -292,7 +305,9 @@ def postprocess(work, reference, pred_vcf_file, output_vcf, candidates_vcf, ense args.lr_snp_min_af, args.lr_ins_min_af, args.lr_del_min_af, args.lr_match_score, args.lr_mismatch_penalty, args.lr_gap_open_penalty, - args.lr_gap_ext_penalty, args.pass_threshold, args.lowqual_threshold, + args.lr_gap_ext_penalty, args.lr_max_realign_dp, + args.lr_do_split, + args.pass_threshold, args.lowqual_threshold, args.msa_binary, args.num_threads) except Exception as e: From 3eed07732f29bd5e18452ab819b417bc42f0b496 Mon Sep 17 00:00:00 2001 From: Sahraeian Date: Sat, 4 Jan 2020 16:54:02 -0800 Subject: [PATCH 03/15] fix realigner --- neusomatic/python/long_read_indelrealign.py | 89 +++++++++++++++------ neusomatic/python/postprocess.py | 2 +- neusomatic/python/resolve_variants.py | 4 +- 3 files changed, 67 insertions(+), 28 deletions(-) diff --git a/neusomatic/python/long_read_indelrealign.py b/neusomatic/python/long_read_indelrealign.py index 382b5f7..535657d 100755 --- a/neusomatic/python/long_read_indelrealign.py +++ b/neusomatic/python/long_read_indelrealign.py @@ -290,7 +290,7 @@ def prepare_fasta(work, region, input_bam, ref_fasta_file, include_ref, split_i, with open(info_file, "w") as info_txt: if include_ref: ref_seq = ref_fasta.fetch( - region.chrom, region.start, region.end + 1) + region.chrom, region.start, region.end + 1).upper() in_fasta.write(">0\n") in_fasta.write("%s\n" % ref_seq.upper()) cnt = 1 @@ -345,7 +345,7 @@ def prepare_fasta(work, region, input_bam, ref_fasta_file, include_ref, split_i, (start_idx - sc_start):(end_idx - sc_start)] non_ins = np.nonzero(positions_ >= 0) refseq = ref_fasta.fetch(region.chrom, positions_[non_ins][0], - positions_[non_ins][-1] + 1) + positions_[non_ins][-1] + 1).upper() q_seq = record.seq[start_idx:end_idx + 1] non_ins_positions = positions_[non_ins] mn, mx = min(non_ins_positions), max( @@ -842,7 +842,7 @@ def do_realign(region, info_file, max_realign_dp, thr_realign=0.0135): return False -def find_var(out_fasta_file, snp_min_af, del_min_af, ins_min_af, scale_maf): +def find_var(out_fasta_file, snp_min_af, del_min_af, ins_min_af, scale_maf, simplify): logger = logging.getLogger(find_var.__name__) records = SeqIO.to_dict(SeqIO.parse(out_fasta_file, "fasta")) if set(map(int, records.keys())) ^ set(range(len(records))): @@ -892,7 +892,27 @@ def find_var(out_fasta_file, snp_min_af, del_min_af, ins_min_af, scale_maf): map(lambda x: NUM_to_NUC[x], filter(lambda x: x > 0, ref_seq))) alt_seq_ = "".join( map(lambda x: NUM_to_NUC[x], filter(lambda x: x > 0, alt_seq))) - return ref_seq_, alt_seq_, afs + if not simplify: + variants = [[0, ref_seq_, alt_seq_, afs]] + else: + variants = [] + bias = 0 + for i, (r, a) in enumerate(zip(ref_seq, alt_seq)): + if r != a: + if i in i_afs: + af = afs[i_afs.index(i)] + else: + af = 0 + rr = "".join(map(lambda x: NUM_to_NUC[ + x], filter(lambda x: x > 0, [r]))) + aa = "".join(map(lambda x: NUM_to_NUC[ + x], filter(lambda x: x > 0, [a]))) + logger.info("{} {} {} {}".format(bias, rr, aa, np.array([af]))) + variants.append([bias, rr, aa, np.array([af])]) + if r != 0: + bias += 1 + + return variants def TrimREFALT(ref, alt, pos): @@ -923,6 +943,7 @@ def run_realignment(input_record): filter_duplicate, \ msa_binary, get_var, do_split = input_record + ref_fasta = pysam.Fastafile(ref_fasta_file) thread_logger = logging.getLogger( "{} ({})".format(run_realignment.__name__, multiprocessing.current_process().name)) @@ -934,7 +955,7 @@ def run_realignment(input_record): if not os.path.exists(pybedtmp): os.mkdir(pybedtmp) pybedtools.set_tempdir(pybedtmp) - variant = [] + variants = [] all_entries = [] input_bam_splits, lens_splits, ds_splits = split_bam_to_chunks( work, region, input_bam, chunk_size, chunk_scale, do_split or not get_var, filter_duplicate) @@ -954,8 +975,10 @@ def run_realignment(input_record): in_fasta_file, match_score, mismatch_penalty, gap_open_penalty, gap_ext_penalty, msa_binary) if get_var: - ref_seq_, alt_seq_, afs = find_var( - out_fasta_file_0, snp_min_af, del_min_af, ins_min_af, scale_maf) + var = find_var( + out_fasta_file_0, snp_min_af, del_min_af, ins_min_af, scale_maf, False) + assert(len(var) == 1) + _, ref_seq_, alt_seq_, afs = var[0] afss.append(afs) new_ref_seq = ref_seq_ new_seqs.append(alt_seq_) @@ -982,26 +1005,41 @@ def run_realignment(input_record): consensus_fasta_aligned = run_msa( consensus_fasta, match_score, mismatch_penalty, gap_open_penalty, gap_ext_penalty, msa_binary) - ref_seq, alt_seq, afs = find_var( - consensus_fasta_aligned, snp_min_af, del_min_af, ins_min_af, 1) - if ref_seq != alt_seq: - ref, alt, pos = TrimREFALT( - ref_seq, alt_seq, int(region.start) + 1) - a = int(np.ceil(np.max(afs) * len(afss))) - af = sum(sorted(map(lambda x: - np.max(x) if x.shape[0] > 0 else 0, - afss))[-a:]) / float(len(afss)) - dp = sum(lens_splits) - ao = int(af * dp) - ro = dp - ao - variant = [region.chrom, pos, ref, alt, dp, ro, ao] + vars_ = find_var( + consensus_fasta_aligned, snp_min_af, del_min_af, ins_min_af, 1, True) + for var in vars_: + pos_, ref_seq, alt_seq, afs = var + if ref_seq != alt_seq: + ref, alt, pos = TrimREFALT( + ref_seq, alt_seq, int(region.start) + 1 + pos_) + a = int(np.ceil(np.max(afs) * len(afss))) + af = sum(sorted(map(lambda x: + np.max(x) if x.shape[0] > 0 else 0, + afss))[-a:]) / float(len(afss)) + dp = sum(lens_splits) + ao = int(af * dp) + ro = dp - ao + if ref == "" and pos > 1: + pos -= 1 + r_ = ref_fasta.fetch( + region.chrom, pos - 1, pos).upper() + ref = r_ + ref + alt = r_ + alt + if alt == "" and pos > 1: + pos -= 1 + r_ = ref_fasta.fetch( + region.chrom, pos - 1, pos).upper() + ref = r_ + ref + alt = r_ + alt + variants.append( + [region.chrom, pos, ref, alt, dp, ro, ao]) else: - if skipped>0 : + if skipped > 0: not_realigned_region = target_region shutil.rmtree(pybedtmp) pybedtools.set_tempdir(original_tempdir) - return all_entries, variant, not_realigned_region + return all_entries, variants, not_realigned_region except Exception as ex: thread_logger.error(traceback.format_exc()) thread_logger.error(ex) @@ -1020,7 +1058,7 @@ def set_chrom(self, chrom): def get_seq(self, start, end=[]): if not end: end = start + 1 - return self.fasta_pysam.fetch(self.chrom, start, end) + return self.fasta_pysam.fetch(self.chrom, start, end).upper() def extend_regions_hp(region_bed_file, extended_region_bed_file, ref_fasta_file, @@ -1260,6 +1298,7 @@ def long_read_indelrealign(work, input_bam, output_bam, output_vcf, output_not_r realign_entries = list(map(lambda x: x[0], realign_output)) realign_variants = list(map(lambda x: x[1], realign_output)) + realign_variants = [v for var in realign_variants for v in var] realign_variants = list(filter(None, realign_variants)) not_realigned_regions = list(map(lambda x: x[2], realign_output)) not_realigned_regions = list(filter(None, not_realigned_regions)) @@ -1373,8 +1412,8 @@ def long_read_indelrealign(work, input_bam, output_bam, output_vcf, output_not_r args.chunk_scale, args.snp_min_af, args.del_min_af, args.ins_min_af, args.match_score, args.mismatch_penalty, args.gap_open_penalty, - args.gap_ext_penalty, - args.gap_ext_penalty, + args.gap_ext_penalty, + args.gap_ext_penalty, args.max_realign_dp, args.do_split, args.filter_duplicate, diff --git a/neusomatic/python/postprocess.py b/neusomatic/python/postprocess.py index c21bcf0..975b178 100755 --- a/neusomatic/python/postprocess.py +++ b/neusomatic/python/postprocess.py @@ -154,8 +154,8 @@ def postprocess(work, reference, pred_vcf_file, output_vcf, candidates_vcf, ense lr_pad, lr_chunk_size, lr_chunk_scale, lr_snp_min_af, lr_ins_min_af, lr_del_min_af, lr_match_score, lr_mismatch_penalty, lr_gap_open_penalty, lr_gap_ext_penalty, lr_max_realign_dp, lr_do_split, - pass_threshold, lowqual_threshold, filter_duplicate, + pass_threshold, lowqual_threshold, msa_binary, num_threads): logger = logging.getLogger(postprocess.__name__) diff --git a/neusomatic/python/resolve_variants.py b/neusomatic/python/resolve_variants.py index 7c58331..b77d8ce 100755 --- a/neusomatic/python/resolve_variants.py +++ b/neusomatic/python/resolve_variants.py @@ -114,7 +114,7 @@ def find_resolved_variants(input_record): list(map(lambda x: x.split("---"), uniq_dels_count.keys())))).saveas() new_bed = new_bed.sort().merge(c=[1], o="count") out_variants.extend(list(map(lambda x: [x[0], int(x[1]), ref.fetch(x[0], int( - x[1]) - 1, int(x[2])), ref.fetch(x[0], int(x[1]) - 1, int(x[1])), "0/1", score], new_bed))) + x[1]) - 1, int(x[2])).upper(), ref.fetch(x[0], int(x[1]) - 1, int(x[1])).upper(), "0/1", score], new_bed))) elif vartype == "INS": intervals = [] inss = [] @@ -143,7 +143,7 @@ def find_resolved_variants(input_record): new_bed = pybedtools.BedTool(map(lambda x: pybedtools.Interval(x[0], int(x[1]), int(x[2]), x[3]), list(map(lambda x: x.split("---"), uniq_inss_count.keys())))).sort() out_variants.extend(list(map(lambda x: [x[0], int(x[1]), ref.fetch(x[0], int( - x[1]) - 1, int(x[1])), ref.fetch(x[0], int(x[1]) - 1, int(x[1])) + x[3], "0/1", score], new_bed))) + x[1]) - 1, int(x[1])).upper(), ref.fetch(x[0], int(x[1]) - 1, int(x[1])).upper() + x[3], "0/1", score], new_bed))) return out_variants except Exception as ex: thread_logger.error(traceback.format_exc()) From 2d4c633525c3a38624b9feffba21b5dac68d056f Mon Sep 17 00:00:00 2001 From: Sahraeian Date: Tue, 18 Feb 2020 13:48:03 -0800 Subject: [PATCH 04/15] few fixes --- neusomatic/python/call.py | 6 ++ neusomatic/python/long_read_indelrealign.py | 67 ++++++++++++++++----- neusomatic/python/train.py | 5 ++ 3 files changed, 64 insertions(+), 14 deletions(-) diff --git a/neusomatic/python/call.py b/neusomatic/python/call.py index d2fc754..073d674 100755 --- a/neusomatic/python/call.py +++ b/neusomatic/python/call.py @@ -11,6 +11,7 @@ import shutil import pickle import multiprocessing +import copy import pysam import numpy as np @@ -68,6 +69,11 @@ def call_variants(net, vartype_classes, call_loader, out_dir, model_tag, use_cud for data in loader_: (matrices, labels, var_pos_s, var_len_s, non_transformed_matrices), (paths) = data + + paths_ = copy.deepcopy(paths) + del paths + paths = paths_ + matrices = Variable(matrices) iii += 1 j += len(paths[0]) diff --git a/neusomatic/python/long_read_indelrealign.py b/neusomatic/python/long_read_indelrealign.py index 535657d..5072fb7 100755 --- a/neusomatic/python/long_read_indelrealign.py +++ b/neusomatic/python/long_read_indelrealign.py @@ -897,24 +897,55 @@ def find_var(out_fasta_file, snp_min_af, del_min_af, ins_min_af, scale_maf, simp else: variants = [] bias = 0 - for i, (r, a) in enumerate(zip(ref_seq, alt_seq)): - if r != a: - if i in i_afs: - af = afs[i_afs.index(i)] + current_ref = [] + current_alt = [] + current_af = [] + current_bias = 0 + is_ins = False + is_del = False + done = False + for i, (r, a) in enumerate(zip(list(ref_seq)+[0], list(alt_seq)+[0])): + if i in i_afs: + af = afs[i_afs.index(i)] + else: + af = 0 + if r == a: + done=True + else: + if r==0 and a!=0: + if not is_ins: + done=True + elif r!=0 and a==0: + if not is_del: + done=True else: - af = 0 - rr = "".join(map(lambda x: NUM_to_NUC[ - x], filter(lambda x: x > 0, [r]))) - aa = "".join(map(lambda x: NUM_to_NUC[ - x], filter(lambda x: x > 0, [a]))) - logger.info("{} {} {} {}".format(bias, rr, aa, np.array([af]))) - variants.append([bias, rr, aa, np.array([af])]) + done=True + if done: + if current_alt: + rr = "".join(map(lambda x: NUM_to_NUC[ + x], filter(lambda x: x > 0, current_ref))) + aa = "".join(map(lambda x: NUM_to_NUC[ + x], filter(lambda x: x > 0, current_alt))) + variants.append([current_bias, rr, aa, np.array(current_af)]) + done=False + current_ref = [] + current_alt = [] + current_af = [] + current_bias = bias + is_ins = False + is_del = False + done = False + if not done: + current_ref.append(r) + current_alt.append(a) + current_af.append(af) + is_ins = r==0 and a!=0 + is_del = r!=0 and a==0 if r != 0: bias += 1 return variants - def TrimREFALT(ref, alt, pos): logger = logging.getLogger(TrimREFALT.__name__) alte = len(alt) @@ -1010,13 +1041,21 @@ def run_realignment(input_record): for var in vars_: pos_, ref_seq, alt_seq, afs = var if ref_seq != alt_seq: + ref, alt, pos = ref_seq, alt_seq, int(region.start) + 1 + pos_ + if pos > 1: + num_add_before = min(40, pos-1) + before = ref_fasta.fetch(region.chrom, pos - num_add_before, pos-1).upper() + print(before) + pos -= num_add_before-1 + ref = before + ref + alt = before + alt ref, alt, pos = TrimREFALT( - ref_seq, alt_seq, int(region.start) + 1 + pos_) + ref, alt, pos) a = int(np.ceil(np.max(afs) * len(afss))) af = sum(sorted(map(lambda x: np.max(x) if x.shape[0] > 0 else 0, afss))[-a:]) / float(len(afss)) - dp = sum(lens_splits) + dp = int(sum(lens_splits)) ao = int(af * dp) ro = dp - ao if ref == "" and pos > 1: diff --git a/neusomatic/python/train.py b/neusomatic/python/train.py index b85e9af..af9af88 100755 --- a/neusomatic/python/train.py +++ b/neusomatic/python/train.py @@ -8,6 +8,7 @@ import argparse import datetime import logging +import copy import numpy as np import torch @@ -89,6 +90,10 @@ def test(net, epoch, validation_loader, use_cuda): for data in validation_loader: (matrices, labels, _, var_len_s, _), (paths) = data + paths_ = copy.deepcopy(paths) + del paths + paths = paths_ + matrices = Variable(matrices) if use_cuda: matrices = matrices.cuda() From aacdae228b231c60a6be82a4d608b25d87b5e8cb Mon Sep 17 00:00:00 2001 From: Sahraeian Date: Tue, 25 Feb 2020 22:25:02 -0800 Subject: [PATCH 05/15] fix imresize --- neusomatic/python/generate_dataset.py | 65 ++++++++++++++------------- 1 file changed, 35 insertions(+), 30 deletions(-) diff --git a/neusomatic/python/generate_dataset.py b/neusomatic/python/generate_dataset.py index 10422e8..aca925a 100755 --- a/neusomatic/python/generate_dataset.py +++ b/neusomatic/python/generate_dataset.py @@ -17,7 +17,7 @@ import numpy as np import pybedtools import pysam -from scipy.misc import imresize +from PIL import Image from split_bed import split_region from utils import concatenate_vcfs, get_chromosomes_order @@ -520,39 +520,43 @@ def prepare_info_matrices_tabix(ref_file, tumor_count_bed, normal_count_bed, rec else: col_pos_map = {i: int(round(v / float(ncols) * matrix_width)) for i, v in col_pos_map.items()} - tumor_count_matrix = imresize( - tumor_matrix, (5, matrix_width)).astype(int) - bq_tumor_count_matrix = imresize( - bq_tumor_matrix, (5, matrix_width)).astype(int) - mq_tumor_count_matrix = imresize( - mq_tumor_matrix, (5, matrix_width)).astype(int) - st_tumor_count_matrix = imresize( - st_tumor_matrix, (5, matrix_width)).astype(int) - lsc_tumor_count_matrix = imresize( - lsc_tumor_matrix, (5, matrix_width)).astype(int) - rsc_tumor_count_matrix = imresize( - rsc_tumor_matrix, (5, matrix_width)).astype(int) + tumor_count_matrix = np.array(Image.fromarray( + tumor_matrix).resize((matrix_width, 5), 2)).astype(int) + bq_tumor_count_matrix = np.array(Image.fromarray( + bq_tumor_matrix).resize((matrix_width, 5), 2)).astype(int) + mq_tumor_count_matrix = np.array(Image.fromarray( + mq_tumor_matrix).resize((matrix_width, 5), 2)).astype(int) + st_tumor_count_matrix = np.array(Image.fromarray( + st_tumor_matrix).resize((matrix_width, 5), 2)).astype(int) + lsc_tumor_count_matrix = np.array(Image.fromarray( + lsc_tumor_matrix).resize((matrix_width, 5), 2)).astype(int) + rsc_tumor_count_matrix = np.array(Image.fromarray( + rsc_tumor_matrix).resize((matrix_width, 5), 2)).astype(int) + tag_tumor_count_matrices = [] for iii in range(len(tag_tumor_matrices)): tag_tumor_count_matrices.append( - imresize(tag_tumor_matrices[iii], (5, matrix_width)).astype(int)) - normal_count_matrix = imresize( - normal_matrix, (5, matrix_width)).astype(int) - bq_normal_count_matrix = imresize( - bq_normal_matrix, (5, matrix_width)).astype(int) - mq_normal_count_matrix = imresize( - mq_normal_matrix, (5, matrix_width)).astype(int) - st_normal_count_matrix = imresize( - st_normal_matrix, (5, matrix_width)).astype(int) - lsc_normal_count_matrix = imresize( - lsc_normal_matrix, (5, matrix_width)).astype(int) - rsc_normal_count_matrix = imresize( - rsc_normal_matrix, (5, matrix_width)).astype(int) + np.array(Image.fromarray(tag_tumor_matrices[iii]).resize((matrix_width, 5), 2)).astype(int)) + + normal_count_matrix = np.array(Image.fromarray( + normal_matrix).resize((matrix_width, 5), 2)).astype(int) + bq_normal_count_matrix = np.array(Image.fromarray( + bq_normal_matrix).resize((matrix_width, 5), 2)).astype(int) + mq_normal_count_matrix = np.array(Image.fromarray( + mq_normal_matrix).resize((matrix_width, 5), 2)).astype(int) + st_normal_count_matrix = np.array(Image.fromarray( + st_normal_matrix).resize((matrix_width, 5), 2)).astype(int) + lsc_normal_count_matrix = np.array(Image.fromarray( + lsc_normal_matrix).resize((matrix_width, 5), 2)).astype(int) + rsc_normal_count_matrix = np.array(Image.fromarray( + rsc_normal_matrix).resize((matrix_width, 5), 2)).astype(int) + tag_normal_count_matrices = [] for iii in range(len(tag_normal_matrices)): tag_normal_count_matrices.append( - imresize(tag_normal_matrices[iii], (5, matrix_width)).astype(int)) - ref_count_matrix = imresize(ref_matrix, (5, matrix_width)).astype(int) + np.array(Image.fromarray(tag_normal_matrices[iii]).resize((matrix_width, 5), 2)).astype(int)) + ref_count_matrix = np.array(Image.fromarray( + ref_matrix).resize((matrix_width, 5), 2)).astype(int) if int(pos) + rcenter[0] not in col_pos_map: center = min(col_pos_map.values()) + rcenter[0] - 1 + rcenter[1] @@ -989,9 +993,10 @@ def find_records(input_record): continue record = line.strip().split() pos = int(record[1]) - if len(record[3]) != len(record[4]) and min(len(record[3]),len(record[4]))>0 and record[3][0] != record[4][0]: + if len(record[3]) != len(record[4]) and min(len(record[3]), len(record[4])) > 0 and record[3][0] != record[4][0]: if pos > 1: - l_base = fasta_file.fetch(record[0], pos - 2, pos - 1).upper() + l_base = fasta_file.fetch( + record[0], pos - 2, pos - 1).upper() record[3] = l_base + record[3] record[4] = l_base + record[4] pos -= 1 From fd7926c2e2b27611f37f2962f4626b0efda02390 Mon Sep 17 00:00:00 2001 From: Sahraeian Date: Tue, 25 Feb 2020 22:40:09 -0800 Subject: [PATCH 06/15] fix for pybed --- neusomatic/python/postprocess.py | 5 ++++- 1 file changed, 4 insertions(+), 1 deletion(-) diff --git a/neusomatic/python/postprocess.py b/neusomatic/python/postprocess.py index 2ff3541..198a762 100755 --- a/neusomatic/python/postprocess.py +++ b/neusomatic/python/postprocess.py @@ -238,7 +238,10 @@ def postprocess(work, reference, pred_vcf_file, output_vcf, candidates_vcf, ense resolve_scores(tumor_bam, ra_resolved_vcf, target_vcf, resolved_vcf) not_resolved_vcf = os.path.join(work, "candidates_preds.not_ra_resolved.vcf") - pybedtools.BedTool(target_vcf).intersect(not_resolved_bed, u=True).saveas(not_resolved_vcf) + cmd = "bedtools intersect -a {} -b {} -u".format( + target_vcf, not_resolved_bed) + run_bedtools_cmd(cmd, output_fn=not_resolved_vcf, run_logger=logger) + all_no_resolve = concatenate_files( [no_resolve, ensembled_preds, not_resolved_vcf], os.path.join(work, "no_resolve.vcf")) From a5e6dd3fb2591d877933295f5d91c3463e076e30 Mon Sep 17 00:00:00 2001 From: Sahraeian Date: Thu, 27 Feb 2020 18:02:14 -0800 Subject: [PATCH 07/15] fix typo --- README.md | 2 +- neusomatic/python/long_read_indelrealign.py | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index dc0f7fe..a9113ba 100644 --- a/README.md +++ b/README.md @@ -218,7 +218,7 @@ There are two alternative ways to prepare this file: cat <(cat Ensemble.s*.tsv |grep CHROM|head -1) \ <(cat Ensemble.s*.tsv |grep -v CHROM) | sed "s/nan/0/g" > ensemble_ann.tsv ``` - and provide `enemble_ann.tsv` as `--enemble_ann` argument in `preprocess.py` and `postprocess.py`. + and provide `ensemble_ann.tsv` as `--ensemble_tsv` argument in `preprocess.py` and `postprocess.py`. ### NOTE: diff --git a/neusomatic/python/long_read_indelrealign.py b/neusomatic/python/long_read_indelrealign.py index aa907f8..7136c88 100755 --- a/neusomatic/python/long_read_indelrealign.py +++ b/neusomatic/python/long_read_indelrealign.py @@ -1004,7 +1004,7 @@ def run_realignment(input_record): if not os.path.exists(bed_tempdir): os.mkdir(bed_tempdir) tempfile.tempdir = bed_tempdir - variant = [] + variants = [] all_entries = [] input_bam_splits, lens_splits, ds_splits = split_bam_to_chunks( work, region, input_bam, chunk_size, chunk_scale, do_split or not get_var, filter_duplicate) From 39f87c02277e02ab7af66bab54a3ea6a5414573c Mon Sep 17 00:00:00 2001 From: Sahraeian Date: Fri, 6 Mar 2020 13:03:38 -0800 Subject: [PATCH 08/15] fix a typo --- neusomatic/python/train.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/neusomatic/python/train.py b/neusomatic/python/train.py index af9af88..3cc5fac 100755 --- a/neusomatic/python/train.py +++ b/neusomatic/python/train.py @@ -389,7 +389,7 @@ def train_neusomatic(candidates_tsv, validation_candidates_tsv, out_dir, checkpo net.train() len_train_set = sum(none_counts) + sum(var_counts) - logger.info("Number of candidater per epoch: {}".format(len_train_set)) + logger.info("Number of candidates per epoch: {}".format(len_train_set)) print_freq = max(1, int(len_train_set / float(batch_size) / 4.0)) curr_epoch = prev_epochs torch.save({"state_dict": net.state_dict(), From 1cbbf2c9240dcbeb564676054a648ecb262e6916 Mon Sep 17 00:00:00 2001 From: Sahraeian Date: Mon, 9 Mar 2020 14:39:00 -0700 Subject: [PATCH 09/15] fix merge_tsvs --- neusomatic/python/merge_tsvs.py | 7 ++++++- 1 file changed, 6 insertions(+), 1 deletion(-) diff --git a/neusomatic/python/merge_tsvs.py b/neusomatic/python/merge_tsvs.py index d91c501..96ca72c 100755 --- a/neusomatic/python/merge_tsvs.py +++ b/neusomatic/python/merge_tsvs.py @@ -47,7 +47,12 @@ def merge_tsvs(input_tsvs, out, totla_L = 0 for tsv in input_tsvs: - totla_L += len(pickle.load(open(tsv + ".idx", "rb"))) - 1 + if os.path.exists(tsv + "idx"): + totla_L += len(pickle.load(open(tsv + ".idx", "rb"))) - 1 + else: + with open(tsv, "r") as i_f: + for line in i_f: + totla_L += 1 totla_L = max(0, totla_L) candidates_per_tsv = max(candidates_per_tsv, np.ceil( totla_L / float(max_num_tsvs)) + 1) From 74b84ba8b42751318c76cacb8f76bb63948d0f8a Mon Sep 17 00:00:00 2001 From: Sahraeian Date: Thu, 12 Mar 2020 16:24:31 -0700 Subject: [PATCH 10/15] fix for dbSNP --- neusomatic/python/filter_candidates.py | 80 +++++++++----------------- neusomatic/python/preprocess.py | 80 +++++++------------------- 2 files changed, 47 insertions(+), 113 deletions(-) diff --git a/neusomatic/python/filter_candidates.py b/neusomatic/python/filter_candidates.py index d24a54f..1f3e96c 100755 --- a/neusomatic/python/filter_candidates.py +++ b/neusomatic/python/filter_candidates.py @@ -3,6 +3,7 @@ # filter_candidates.py # filter raw candidates extracted by 'scan_alignments.py' using min_af and other cut-offs #------------------------------------------------------------------------- +import os import argparse import traceback import logging @@ -25,6 +26,16 @@ def filter_candidates(candidate_record): thread_logger.info( "---------------------Filter Candidates---------------------") + if dbsnp: + if dbsnp[-6:] != "vcf.gz": + thread_logger.error("Aborting!") + raise Exception( + "The dbSNP file should be a tabix indexed file with .vcf.gz format") + if not os.path.exists(dbsnp + ".tbi"): + thread_logger.error("Aborting!") + raise Exception( + "The dbSNP file should be a tabix indexed file with .vcf.gz format. No {}.tbi file exists.".format(dbsnp)) + records = {} with open(candidates_vcf) as v_f: for line in v_f: @@ -261,64 +272,25 @@ def filter_candidates(candidate_record): final_records.append([chrom, pos - 1, ref, alt, line]) final_records = sorted(final_records, key=lambda x: x[0:2]) if dbsnp: - filtered_bed = tempfile.NamedTemporaryFile( - prefix="tmpbed_", suffix=".bed", delete=False) - filtered_bed = filtered_bed.name - with open(filtered_bed, "w") as f_o: - for x in enumerate(final_records): - f_o.write("\t".join(map(str, [x[1][0], int(x[1][1]), int( - x[1][1]) + 1, x[1][2], x[1][3], str(x[0])])) + "\n") - cmd = "bedtools sort -i {}".format(filtered_bed) - filtered_bed = run_bedtools_cmd(cmd, run_logger=thread_logger) - - dbsnp_tmp = tempfile.NamedTemporaryFile( - prefix="tmpbed_", suffix=".bed", delete=False) - dbsnp_tmp = dbsnp_tmp.name - with open(dbsnp_tmp, "w") as f_o: - with open(dbsnp, "r") as f_i: - for line in f_i: - if not line.strip(): - continue - if line[0] == "#": - continue - x = line.strip().split("\t") - f_o.write( - "\t".join(map(str, [x[0], int(x[1]), int(x[1]) + 1, x[3], x[4]])) + "\n") - cmd = "bedtools sort -i {}".format(dbsnp_tmp) - run_bedtools_cmd(cmd, output_fn=dbsnp, run_logger=thread_logger) - - cmd = "bedtools window -a {} -b {} -w 0 -v".format( - filtered_bed, dbsnp) - non_in_dbsnp_1 = run_bedtools_cmd(cmd, run_logger=thread_logger) - cmd = "bedtools window -a {} -b {} -w 0".format( - filtered_bed, dbsnp) - non_in_dbsnp_2 = run_bedtools_cmd(cmd, run_logger=thread_logger) - cmd = '''awk '($2!=$8)||($4!=$10)||($5!=$11){{print $0}}' {}'''.format( - non_in_dbsnp_2) - non_in_dbsnp_2 = run_bedtools_cmd(cmd, run_logger=thread_logger) - cmd = "bedtools sort -i {}".format(non_in_dbsnp_2) - non_in_dbsnp_2 = run_bedtools_cmd(cmd, run_logger=thread_logger) - - non_in_dbsnp_ids = [] - with open(non_in_dbsnp_1) as i_f: - for line in i_f: - if not line.strip(): - continue - x = line.strip().split("\t") - non_in_dbsnp_ids.append(int(x[5])) - with open(non_in_dbsnp_2) as i_f: - for line in i_f: - if not line.strip(): - continue - x = line.strip().split("\t") - non_in_dbsnp_ids.append(int(x[5])) - final_records = list(map(lambda x: x[1], filter( - lambda x: x[0] in non_in_dbsnp_ids, enumerate(final_records)))) + dbsnp_tb = pysam.TabixFile(dbsnp) with open(filtered_candidates_vcf, "w") as o_f: o_f.write("##fileformat=VCFv4.2\n") o_f.write( "#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT\tSAMPLE\n") for record in final_records: + if dbsnp: + chrom, pos, ref, alt = record[0:4] + var_id = "-".join(map(str,[chrom, pos, ref, alt])) + region = "{}:{}-{}".format(chrom, pos, pos + 1) + dbsnp_vars = [] + for x in dbsnp_tb.fetch(region=region): + chrom_, pos_, _, ref_, alts_ = x.strip().split("\t")[ + 0:5] + for alt_ in alts_.split(","): + dbsnp_var_id = "-".join(map(str,[chrom_, pos_, ref_, alt_])) + dbsnp_vars.append(dbsnp_var_id) + if var_id in dbsnp_vars: + continue o_f.write(record[-1] + "\n") return filtered_candidates_vcf @@ -341,7 +313,7 @@ def filter_candidates(candidate_record): parser.add_argument('--reference', type=str, help='reference fasta filename', required=True) parser.add_argument('--dbsnp_to_filter', type=str, - help='dbsnp vcf (will be used to filter candidate variants)', default=None) + help='dbsnp vcf.gz (will be used to filter candidate variants)', default=None) parser.add_argument('--good_ao', type=float, help='good alternate count (ignores maf)', default=10) parser.add_argument('--min_ao', type=float, diff --git a/neusomatic/python/preprocess.py b/neusomatic/python/preprocess.py index b227def..91596b9 100755 --- a/neusomatic/python/preprocess.py +++ b/neusomatic/python/preprocess.py @@ -22,22 +22,6 @@ from utils import concatenate_vcfs, run_bedtools_cmd -def split_dbsnp(record): - restart, dbsnp, region_bed, dbsnp_region_vcf = record - thread_logger = logging.getLogger( - "{} ({})".format(split_dbsnp.__name__, multiprocessing.current_process().name)) - try: - if restart or not os.path.exists(dbsnp_region_vcf): - cmd = "bedtools intersect -a {} -b {} -u".format(dbsnp, region_bed) - run_bedtools_cmd(cmd, output_fn=dbsnp_region_vcf, - run_logger=thread_logger) - return dbsnp_region_vcf - except Exception as ex: - thread_logger.error(traceback.format_exc()) - thread_logger.error(ex) - return None - - def process_split_region(tn, work, region, reference, mode, alignment_bam, dbsnp, scan_window_size, scan_maf, min_mapq, filtered_candidates_vcf, min_dp, max_dp, @@ -45,7 +29,7 @@ def process_split_region(tn, work, region, reference, mode, alignment_bam, dbsnp good_ao, min_ao, snp_min_af, snp_min_bq, snp_min_ao, ins_min_af, del_min_af, del_merge_min_af, ins_merge_min_af, merge_r, - scan_alignments_binary, restart, num_threads, calc_qual, regions=[], dbsnp_regions=[]): + scan_alignments_binary, restart, num_threads, calc_qual, regions=[]): logger = logging.getLogger(process_split_region.__name__) logger.info("Scan bam.") @@ -56,36 +40,12 @@ def process_split_region(tn, work, region, reference, mode, alignment_bam, dbsnp if filtered_candidates_vcf: logger.info("Filter candidates.") if restart or not os.path.exists(filtered_candidates_vcf): - if dbsnp and not dbsnp_regions: - map_args = [] - for raw_vcf, count_bed, split_region_bed in scan_outputs: - dbsnp_region_vcf = os.path.join(os.path.dirname( - os.path.realpath(raw_vcf)), "dbsnp_region.vcf") - map_args.append( - (restart, dbsnp, split_region_bed, dbsnp_region_vcf)) - pool = multiprocessing.Pool(num_threads) - try: - dbsnp_regions = pool.map_async(split_dbsnp, map_args).get() - pool.close() - except Exception as inst: - logger.error(inst) - pool.close() - traceback.print_exc() - raise Exception - - for o in dbsnp_regions: - if o is None: - raise Exception("split_dbsnp failed!") - pool = multiprocessing.Pool(num_threads) map_args = [] for i, (raw_vcf, count_bed, split_region_bed) in enumerate(scan_outputs): filtered_vcf = os.path.join(os.path.dirname( os.path.realpath(raw_vcf)), "filtered_candidates.vcf") - dbsnp_region_vcf = None - if dbsnp: - dbsnp_region_vcf = dbsnp_regions[i] - map_args.append((raw_vcf, filtered_vcf, reference, dbsnp_region_vcf, min_dp, max_dp, good_ao, + map_args.append((raw_vcf, filtered_vcf, reference, dbsnp, min_dp, max_dp, good_ao, min_ao, snp_min_af, snp_min_bq, snp_min_ao, ins_min_af, del_min_af, del_merge_min_af, ins_merge_min_af, merge_r)) try: @@ -111,15 +71,9 @@ def process_split_region(tn, work, region, reference, mode, alignment_bam, dbsnp filtered_vcf = os.path.join(os.path.dirname( os.path.realpath(raw_vcf)), "filtered_candidates.vcf") filtered_candidates_vcfs.append(filtered_vcf) - if dbsnp and not dbsnp_regions: - dbsnp_regions = [] - for raw_vcf, _, _ in scan_outputs: - dbsnp_region_vcf = os.path.join(os.path.dirname( - os.path.realpath(raw_vcf)), "dbsnp_region.vcf") - dbsnp_regions.append(dbsnp_region_vcf) else: filtered_candidates_vcfs = None - return list(map(lambda x: x[1], scan_outputs)), list(map(lambda x: x[2], scan_outputs)), filtered_candidates_vcfs, dbsnp_regions + return list(map(lambda x: x[1], scan_outputs)), list(map(lambda x: x[2], scan_outputs)), filtered_candidates_vcfs def generate_dataset_region(work, truth_vcf, mode, filtered_candidates_vcf, region, tumor_count_bed, normal_count_bed, reference, @@ -284,6 +238,17 @@ def preprocess(work, mode, reference, region_bed, tumor_bam, normal_bam, dbsnp, raise Exception( "No normal .bai index file {}".format(normal_bam + ".bai")) + if dbsnp: + if dbsnp[-6:] != "vcf.gz": + logger.error("Aborting!") + raise Exception( + "The dbSNP file should be a tabix indexed file with .vcf.gz format") + if not os.path.exists(dbsnp + ".tbi"): + logger.error("Aborting!") + raise Exception( + "The dbSNP file should be a tabix indexed file with .vcf.gz format. No {}.tbi file exists.".format(dbsnp)) + + ensemble_bed = None if ensemble_tsv: ensemble_bed = os.path.join(work, "ensemble.bed") @@ -293,7 +258,6 @@ def preprocess(work, mode, reference, region_bed, tumor_bam, normal_bam, dbsnp, merge_d_for_short_read = 100 candidates_split_regions = [] - dbsnp_regions_q = [] ensemble_beds = [] if not long_read and first_do_without_qual: logger.info("Scan tumor bam (first without quality scores).") @@ -312,8 +276,8 @@ def preprocess(work, mode, reference, region_bed, tumor_bam, normal_bam, dbsnp, ins_min_af, del_min_af, del_merge_min_af, ins_merge_min_af, merge_r, scan_alignments_binary, restart, num_threads, - calc_qual=False, dbsnp_regions=[]) - tumor_counts_without_q, split_regions, filtered_candidates_vcfs_without_q, dbsnp_regions_q = tumor_outputs_without_q + calc_qual=False) + tumor_counts_without_q, split_regions, filtered_candidates_vcfs_without_q = tumor_outputs_without_q if ensemble_tsv: ensemble_beds = get_ensemble_beds( @@ -338,9 +302,8 @@ def preprocess(work, mode, reference, region_bed, tumor_bam, normal_bam, dbsnp, ins_merge_min_af, merge_r, scan_alignments_binary, restart, num_threads, calc_qual=True, - regions=candidates_split_regions, - dbsnp_regions=dbsnp_regions_q) - tumor_counts, split_regions, filtered_candidates_vcfs, _ = tumor_outputs + regions=candidates_split_regions) + tumor_counts, split_regions, filtered_candidates_vcfs = tumor_outputs if ensemble_tsv and not ensemble_beds: ensemble_beds = get_ensemble_beds( @@ -357,7 +320,7 @@ def preprocess(work, mode, reference, region_bed, tumor_bam, normal_bam, dbsnp, if restart or not os.path.exists(work_normal): os.mkdir(work_normal) logger.info("Scan normal bam (and extracting quality scores).") - normal_counts, _, _, _ = process_split_region("normal", work_normal, region_bed, reference, mode, normal_bam, + normal_counts, _, _ = process_split_region("normal", work_normal, region_bed, reference, mode, normal_bam, None, scan_window_size, 0.2, min_mapq, None, min_dp, max_dp, filter_duplicate, @@ -366,8 +329,7 @@ def preprocess(work, mode, reference, region_bed, tumor_bam, normal_bam, dbsnp, ins_merge_min_af, merge_r, scan_alignments_binary, restart, num_threads, calc_qual=True, - regions=candidates_split_regions, - dbsnp_regions=[]) + regions=candidates_split_regions) work_dataset = os.path.join(work, "dataset") if restart or not os.path.exists(work_dataset): @@ -411,7 +373,7 @@ def preprocess(work, mode, reference, region_bed, tumor_bam, normal_bam, dbsnp, parser.add_argument('--work', type=str, help='work directory', required=True) parser.add_argument('--dbsnp_to_filter', type=str, - help='dbsnp vcf (will be used to filter candidate variants)', default=None) + help='dbsnp vcf.gz (will be used to filter candidate variants)', default=None) parser.add_argument('--scan_window_size', type=int, help='window size to scan the variants', default=2000) parser.add_argument('--scan_maf', type=float, From ab4779e273d1ff6cd079544e9fd94bc0f986e656 Mon Sep 17 00:00:00 2001 From: Sahraeian Date: Thu, 9 Apr 2020 23:53:14 -0700 Subject: [PATCH 11/15] fix test --- test/aa/dataset/work.0/candidates_0.tsv | 27 --- test/aa/dataset/work.0/candidates_0.tsv.idx | Bin 91 -> 0 bytes test/aa/dataset/work.0/done.txt | 1 - test/aa/dataset/work.0/none.vcf | 19 --- test/aa/dataset/work.0/pred_0.vcf | 27 --- test/aa/dataset/work.0/region_0.bed | 24 --- test/aa/dataset/work.0/truth_0.vcf | 8 - test/aa/dataset/work.0/var.vcf | 8 - test/aa/work_normal/work.0/candidates.vcf | 18 -- test/aa/work_normal/work.0/count.bed.gz | Bin 11821 -> 0 bytes test/aa/work_normal/work.0/count.bed.gz.tbi | Bin 242 -> 0 bytes test/aa/work_normal/work.0/region.bed | 24 --- test/aa/work_normal/work.0/scan.err | 3 - test/aa/work_normal/work.0/scan.out | 0 test/aa/work_tumor/all_regions.bed | 154 ------------------ test/aa/work_tumor/candidates_region_0.bed | 24 --- test/aa/work_tumor/filtered_candidates.vcf | 29 ---- test/aa/work_tumor/region_0.bed | 154 ------------------ test/aa/work_tumor/work.0/candidates.vcf | 43 ----- test/aa/work_tumor/work.0/count.bed.gz | Bin 663573 -> 0 bytes test/aa/work_tumor/work.0/count.bed.gz.tbi | Bin 424 -> 0 bytes .../work_tumor/work.0/filtered_candidates.vcf | 29 ---- test/aa/work_tumor/work.0/region.bed | 154 ------------------ test/aa/work_tumor/work.0/scan.err | 5 - test/aa/work_tumor/work.0/scan.out | 0 test/t.vcf | 24 --- 26 files changed, 775 deletions(-) delete mode 100644 test/aa/dataset/work.0/candidates_0.tsv delete mode 100644 test/aa/dataset/work.0/candidates_0.tsv.idx delete mode 100644 test/aa/dataset/work.0/done.txt delete mode 100644 test/aa/dataset/work.0/none.vcf delete mode 100644 test/aa/dataset/work.0/pred_0.vcf delete mode 100644 test/aa/dataset/work.0/region_0.bed delete mode 100644 test/aa/dataset/work.0/truth_0.vcf delete mode 100644 test/aa/dataset/work.0/var.vcf delete mode 100644 test/aa/work_normal/work.0/candidates.vcf delete mode 100644 test/aa/work_normal/work.0/count.bed.gz delete mode 100644 test/aa/work_normal/work.0/count.bed.gz.tbi delete mode 100644 test/aa/work_normal/work.0/region.bed delete mode 100644 test/aa/work_normal/work.0/scan.err delete mode 100644 test/aa/work_normal/work.0/scan.out delete mode 100644 test/aa/work_tumor/all_regions.bed delete mode 100644 test/aa/work_tumor/candidates_region_0.bed delete mode 100644 test/aa/work_tumor/filtered_candidates.vcf delete mode 100644 test/aa/work_tumor/region_0.bed delete mode 100644 test/aa/work_tumor/work.0/candidates.vcf delete mode 100644 test/aa/work_tumor/work.0/count.bed.gz delete mode 100644 test/aa/work_tumor/work.0/count.bed.gz.tbi delete mode 100644 test/aa/work_tumor/work.0/filtered_candidates.vcf delete mode 100644 test/aa/work_tumor/work.0/region.bed delete mode 100644 test/aa/work_tumor/work.0/scan.err delete mode 100644 test/aa/work_tumor/work.0/scan.out delete mode 100644 test/t.vcf diff --git a/test/aa/dataset/work.0/candidates_0.tsv b/test/aa/dataset/work.0/candidates_0.tsv deleted file 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A C . . TAG=0.21172873.A.C.NONE.15.0.92.36; . . -22 21213060 . C G . . TAG=0.21213060.C.G.NONE.15.0.224.103; . . -22 21330465 . CCAT C . . TAG=0.21330465.CCAT.C.NONE.16.0.165.81; . . -22 21330608 . C T . . TAG=0.21330608.C.T.NONE.15.0.278.151; . . -22 21337325 . G A . . TAG=0.21337325.G.A.NONE.15.0.106.42; . . -22 21341676 . T A . . TAG=0.21341676.T.A.NONE.15.0.42.16; . . -22 21341677 . C CA . . TAG=0.21341677.C.CA.NONE.16.0.43.17; . . -22 21341677 . CA C . . TAG=0.21341677.CA.C.NONE.16.0.43.17; . . -22 21377334 . C T . . TAG=0.21377334.C.T.NONE.15.0.358.147; . . -22 21380064 . G A . . TAG=0.21380064.G.A.NONE.15.0.226.92; . . -22 21380530 . G T . . TAG=0.21380530.G.T.NONE.15.0.37.17; . . -22 21384578 . C T . . TAG=0.21384578.C.T.NONE.15.0.86.31; . . -22 21386019 . G A . . TAG=0.21386019.G.A.NONE.15.0.159.76; . . -22 21968716 . CA C . . TAG=0.21968716.CA.C.NONE.16.0.136.71; . . -22 21983853 . G A . . TAG=0.21983853.G.A.NONE.15.0.374.191; . . -22 21984290 . C A . . TAG=0.21984290.C.A.NONE.15.0.8.2; . . -22 21988524 . C T . . TAG=0.21988524.C.T.NONE.15.0.219.99; . . -22 21988602 . C T . . TAG=0.21988602.C.T.NONE.15.0.212.106; . . -22 21991072 . T C . . TAG=0.21991072.T.C.NONE.15.0.114.47; . . diff --git a/test/aa/dataset/work.0/pred_0.vcf b/test/aa/dataset/work.0/pred_0.vcf deleted file mode 100644 index aa586da..0000000 --- a/test/aa/dataset/work.0/pred_0.vcf +++ /dev/null @@ -1,27 +0,0 @@ -22 21172873 . A C 100 . DP=85;RO=61;AO=24;ST=29,10;LS=0;RS=0;NM=99;AS=96;XS=85;PR=100;CL=0;MQ=60;BQ=37;AF=0.2824 GT:DP:RO:AO:AF 0/1:85:61:24:0.2824 -22 21213060 . C G 100 . DP=208;RO=160;AO=48;ST=90,26;LS=0;RS=0;NM=99;AS=94;XS=87;PR=100;CL=15;MQ=60;BQ=34;AF=0.2308 GT:DP:RO:AO:AF 0/1:208:160:48:0.2308 -22 21330465 . CCAT C 100 . DP=134;RO=105;AO=29;ST=77,19;LS=0;RS=0;NM=84;AS=77;XS=83;PR=97;CL=7;MQ=60;BQ=36;AF=0.2164 GT:DP:RO:AO:AF 0/1:134:105:29:0.2164 -22 21330608 . C T 100 . DP=271;RO=198;AO=73;ST=117,40;LS=0;RS=0;NM=99;AS=95;XS=85;PR=100;CL=3;MQ=60;BQ=32;AF=0.2694 GT:DP:RO:AO:AF 0/1:271:198:73:0.2694 -22 21330787 . C T 100 . DP=396;RO=306;AO=88;ST=147,37;LS=0;RS=0;NM=99;AS=95;XS=89;PR=99;CL=9;MQ=60;BQ=33;AF=0.2234 GT:DP:RO:AO:AF 0/1:396:306:88:0.2234 -22 21332122 . G A 100 . DP=285;RO=223;AO=62;ST=129,39;LS=0;RS=0;NM=99;AS=95;XS=85;PR=100;CL=5;MQ=60;BQ=33;AF=0.2175 GT:DP:RO:AO:AF 0/1:285:223:62:0.2175 -22 21334924 . G C 100 . DP=106;RO=83;AO=23;ST=62,16;LS=0;RS=0;NM=98;AS=94;XS=91;PR=100;CL=4;MQ=60;BQ=37;AF=0.217 GT:DP:RO:AO:AF 0/1:106:83:23:0.217 -22 21335259 . C A 100 . DP=249;RO=200;AO=49;ST=103,26;LS=0;RS=0;NM=99;AS=96;XS=85;PR=100;CL=0;MQ=60;BQ=32;AF=0.1968 GT:DP:RO:AO:AF 0/1:249:200:49:0.1968 -22 21337325 . G A 100 . DP=98;RO=76;AO=22;ST=43,14;LS=0;RS=0;NM=99;AS=94;XS=84;PR=100;CL=5;MQ=60;BQ=33;AF=0.2245 GT:DP:RO:AO:AF 0/1:98:76:22:0.2245 -22 21341676 . T A 100 . DP=21;RO=18;AO=3;ST=14,1;LS=0;RS=0;NM=97;AS=83;XS=72;PR=100;CL=33;MQ=60;BQ=25;AF=0.1429 GT:DP:RO:AO:AF 0/1:21:18:3:0.1429 -22 21341677 . C CA 100 . DP=23;RO=21;AO=2;ST=14,2;LS=0;RS=0;NM=94;AS=66;XS=71;PR=100;CL=50;MQ=60;BQ=34;AF=0.087 GT:DP:RO:AO:AF 0/1:23:21:2:0.087 -22 21341677 . CA C 100 . DP=24;RO=20;AO=4;ST=13,4;LS=0;RS=0;NM=98;AS=72;XS=64;PR=100;CL=25;MQ=58;BQ=32;AF=0.1667 GT:DP:RO:AO:AF 0/1:24:20:4:0.1667 -22 21377334 . C T 100 . DP=353;RO=279;AO=74;ST=152,36;LS=0;RS=0;NM=98;AS=90;XS=84;PR=100;CL=8;MQ=60;BQ=34;AF=0.2096 GT:DP:RO:AO:AF 0/1:353:279:74:0.2096 -22 21380064 . G A 100 . DP=206;RO=159;AO=47;ST=103,30;LS=0;RS=0;NM=99;AS=95;XS=92;PR=100;CL=19;MQ=60;BQ=34;AF=0.2282 GT:DP:RO:AO:AF 0/1:206:159:47:0.2282 -22 21380530 . G T 100 . DP=34;RO=32;AO=2;ST=23,1;LS=0;RS=0;NM=99;AS=92;XS=84;PR=100;CL=100;MQ=60;BQ=34;AF=0.0588 GT:DP:RO:AO:AF 0/1:34:32:2:0.0588 -22 21384516 . C T 100 . DP=95;RO=68;AO=27;ST=24,7;LS=0;RS=0;NM=99;AS=95;XS=86;PR=100;CL=11;MQ=60;BQ=35;AF=0.2842 GT:DP:RO:AO:AF 0/1:95:68:27:0.2842 -22 21384578 . C T 100 . DP=81;RO=58;AO=23;ST=25,9;LS=0;RS=0;NM=99;AS=94;XS=85;PR=100;CL=13;MQ=60;BQ=32;AF=0.284 GT:DP:RO:AO:AF 0/1:81:58:23:0.284 -22 21386019 . G A 100 . DP=156;RO=124;AO=32;ST=41,13;LS=0;RS=0;NM=99;AS=95;XS=87;PR=100;CL=0;MQ=60;BQ=33;AF=0.2051 GT:DP:RO:AO:AF 0/1:156:124:32:0.2051 -22 21968716 . CA C 100 . DP=109;RO=91;AO=17;ST=65,13;LS=0;RS=0;NM=98;AS=85;XS=70;PR=94;CL=35;MQ=61;BQ=31;AF=0.1574 GT:DP:RO:AO:AF 0/1:109:91:17:0.1574 -22 21982892 . C T 100 . DP=158;RO=113;AO=45;ST=58,23;LS=0;RS=0;NM=99;AS=96;XS=99;PR=100;CL=0;MQ=60;BQ=33;AF=0.2848 GT:DP:RO:AO:AF 0/1:158:113:45:0.2848 -22 21983260 . A G 100 . DP=118;RO=74;AO=44;ST=41,29;LS=0;RS=0;NM=99;AS=95;XS=99;PR=100;CL=0;MQ=60;BQ=33;AF=0.3729 GT:DP:RO:AO:AF 0/1:118:74:44:0.3729 -22 21983853 . G A 100 . DP=337;RO=249;AO=87;ST=81,35;LS=0;RS=0;NM=99;AS=95;XS=95;PR=100;CL=10;MQ=60;BQ=31;AF=0.2589 GT:DP:RO:AO:AF 0/1:337:249:87:0.2589 -22 21984290 . C A 100 . DP=8;RO=7;AO=1;ST=2,0;LS=0;RS=0;NM=99;AS=96;XS=85;PR=100;CL=0;MQ=60;BQ=36;AF=0.125 GT:DP:RO:AO:AF 0/1:8:7:1:0.125 -22 21988524 . C T 100 . DP=217;RO=167;AO=50;ST=95,31;LS=0;RS=0;NM=99;AS=93;XS=88;PR=98;CL=14;MQ=60;BQ=33;AF=0.2304 GT:DP:RO:AO:AF 0/1:217:167:50:0.2304 -22 21988602 . C T 100 . DP=203;RO=162;AO=41;ST=91,21;LS=0;RS=0;NM=99;AS=95;XS=98;PR=98;CL=5;MQ=60;BQ=34;AF=0.202 GT:DP:RO:AO:AF 0/1:203:162:41:0.202 -22 21989959 . AAG A 100 . DP=139;RO=107;AO=32;ST=33,11;LS=0;RS=0;NM=98;AS=93;XS=91;PR=100;CL=6;MQ=61;BQ=35;AF=0.2302 GT:DP:RO:AO:AF 0/1:139:107:32:0.2302 -22 21991072 . T C 100 . DP=109;RO=83;AO=26;ST=44,14;LS=0;RS=0;NM=99;AS=96;XS=86;PR=100;CL=0;MQ=60;BQ=32;AF=0.2385 GT:DP:RO:AO:AF 0/1:109:83:26:0.2385 diff --git a/test/aa/dataset/work.0/region_0.bed b/test/aa/dataset/work.0/region_0.bed deleted file mode 100644 index f43f621..0000000 --- a/test/aa/dataset/work.0/region_0.bed +++ /dev/null @@ -1,24 +0,0 @@ -22 21988514 21988605 . . . -22 21384506 21384589 . . . -22 21380520 21380541 . . . -22 21983843 21983864 . . . -22 21332112 21332133 . . . -22 21377324 21377345 . . . -22 21386009 21386030 . . . -22 21334919 21334935 . . . -22 21213050 21213071 . . . -22 21968712 21968728 . . . -22 21380056 21380075 . . . -22 21330465 21330479 . . . -22 21984280 21984293 . . . -22 21337315 21337336 . . . -22 21330598 21330619 . . . -22 21991062 21991083 . . . -22 21335249 21335270 . . . -22 21989949 21989972 . . . -22 21982882 21982903 . . . -22 21988606 21988613 . . . -22 21330777 21330798 . . . -22 21341673 21341689 . . . -22 21172863 21172884 . . . -22 21983250 21983271 . . . diff --git a/test/aa/dataset/work.0/truth_0.vcf b/test/aa/dataset/work.0/truth_0.vcf deleted file mode 100644 index ec007d0..0000000 --- a/test/aa/dataset/work.0/truth_0.vcf +++ /dev/null @@ -1,8 +0,0 @@ -22 21330787 . c t 26.9917 PASS SCORE=0.9980;DP=396;RO=306;AO=88;AF=0.2234 GT:DP:RO:AO:AF 0/1:396:306:88:0.2234 -22 21332122 . G A 28.5402 PASS SCORE=0.9986;DP=285;RO=223;AO=62;AF=0.2175 GT:DP:RO:AO:AF 0/1:285:223:62:0.2175 -22 21334924 . G C 17.5639 PASS SCORE=0.9825;DP=106;RO=83;AO=23;AF=0.217 GT:DP:RO:AO:AF 0/1:106:83:23:0.217 -22 21335259 . C A 19.7149 PASS SCORE=0.9893;DP=249;RO=200;AO=49;AF=0.1968 GT:DP:RO:AO:AF 0/1:249:200:49:0.1968 -22 21384516 . C T 27.6969 PASS SCORE=0.9983;DP=95;RO=68;AO=27;AF=0.2842 GT:DP:RO:AO:AF 0/1:95:68:27:0.2842 -22 21982892 . C T 21.5561 PASS SCORE=0.9930;DP=158;RO=113;AO=45;AF=0.2848 GT:DP:RO:AO:AF 0/1:158:113:45:0.2848 -22 21983260 . A G 31.5494 PASS SCORE=0.9993;DP=118;RO=74;AO=44;AF=0.3729 GT:DP:RO:AO:AF 0/1:118:74:44:0.3729 -22 21989959 . AAG A 33.0106 PASS SCORE=0.9995;DP=139;RO=107;AO=32;AF=0.2302 GT:DP:RO:AO:AF 0/1:139:107:32:0.2302 diff --git a/test/aa/dataset/work.0/var.vcf b/test/aa/dataset/work.0/var.vcf deleted file mode 100644 index 51e1ce3..0000000 --- a/test/aa/dataset/work.0/var.vcf +++ /dev/null @@ -1,8 +0,0 @@ -22 21989959 . AAG A . . TAG=0.21989959.AAG.A.DEL.16.2.154.80; . . -22 21330787 . C T . . TAG=0.21330787.C.T.SNP.15.1.405.183; . . -22 21332122 . G A . . TAG=0.21332122.G.A.SNP.15.1.292.148; . . -22 21334924 . G C . . TAG=0.21334924.G.C.SNP.15.1.117.65; . . -22 21335259 . C A . . TAG=0.21335259.C.A.SNP.15.1.253.142; . . -22 21384516 . C T . . TAG=0.21384516.C.T.SNP.15.1.101.48; . . -22 21982892 . C T . . TAG=0.21982892.C.T.SNP.15.1.161.73; . . -22 21983260 . A G . . TAG=0.21983260.A.G.SNP.15.1.119.65; . . diff --git a/test/aa/work_normal/work.0/candidates.vcf b/test/aa/work_normal/work.0/candidates.vcf deleted file mode 100644 index f07d907..0000000 --- a/test/aa/work_normal/work.0/candidates.vcf +++ /dev/null @@ -1,18 +0,0 @@ -##fileformat=VCFv4.2 -##source=VarCal -##reference=example/Homo_sapiens.GRCh37.75.dna.chromosome.22.fa -##INFO= -#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT -22 21172873 . A C 100 . AF=0.464286;DP=28;RO=15;AO=13;ST=8,8;LS=0;RS=0;NM=99;AS=94;XS=85;PR=100;CL=0;MQ=60;BQ=37 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:28:15:13:8,8:0:0:99:94:85:100:0:60:37 -22 21213060 . C G 100 . AF=0.459184;DP=98;RO=53;AO=45;ST=26,25;LS=0;RS=0;NM=99;AS=94;XS=85;PR=100;CL=18;MQ=60;BQ=33 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:98:53:45:26,25:0:0:99:94:85:100:18:60:33 -22 21330608 . C T 100 . AF=0.523490;DP=149;RO=71;AO=78;ST=44,43;LS=0;RS=0;NM=99;AS=95;XS=86;PR=100;CL=3;MQ=60;BQ=32 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:149:71:78:44,43:0:0:99:95:86:100:3:60:32 -22 21337325 . G A 100 . AF=0.550000;DP=40;RO=18;AO=22;ST=9,15;LS=0;RS=0;NM=98;AS=93;XS=84;PR=100;CL=5;MQ=60;BQ=32 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:40:18:22:9,15:0:0:98:93:84:100:5:60:32 -22 21341677 . C A 100 . AF=0.285714;DP=7;RO=5;AO=2;ST=5,1;LS=0;RS=0;NM=98;AS=90;XS=72;PR=100;CL=50;MQ=60;BQ=24 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:7:5:2:5,1:0:0:98:90:72:100:50:60:24 -22 21377334 . C T 100 . AF=0.544776;DP=134;RO=61;AO=73;ST=34,36;LS=0;RS=0;NM=98;AS=89;XS=84;PR=100;CL=19;MQ=60;BQ=33 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:134:61:73:34,36:0:0:98:89:84:100:19:60:33 -22 21380064 . G A 100 . AF=0.385542;DP=83;RO=51;AO=32;ST=32,17;LS=0;RS=0;NM=99;AS=94;XS=90;PR=100;CL=13;MQ=60;BQ=35 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:83:51:32:32,17:0:0:99:94:90:100:13:60:35 -22 21384578 . C T 100 . AF=0.517241;DP=29;RO=14;AO=15;ST=7,7;LS=0;RS=0;NM=99;AS=90;XS=87;PR=100;CL=33;MQ=60;BQ=31 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:29:14:15:7,7:0:0:99:90:87:100:33:60:31 -22 21386019 . G A 100 . AF=0.355263;DP=76;RO=49;AO=27;ST=19,12;LS=0;RS=0;NM=99;AS=93;XS=87;PR=100;CL=4;MQ=60;BQ=33 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:76:49:27:19,12:0:0:99:93:87:100:4:60:33 -22 21983853 . G A 100 . AF=0.494318;DP=177;RO=89;AO=87;ST=35,36;LS=0;RS=0;NM=99;AS=95;XS=95;PR=100;CL=3;MQ=60;BQ=32 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:177:89:87:35,36:0:0:99:95:95:100:3:60:32 -22 21988524 . C T 100 . AF=0.591837;DP=98;RO=40;AO=58;ST=25,33;LS=0;RS=0;NM=99;AS=94;XS=87;PR=100;CL=9;MQ=60;BQ=34 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:98:40:58:25,33:0:0:99:94:87:100:9:60:34 -22 21988602 . C T 100 . AF=0.469388;DP=98;RO=52;AO=46;ST=32,27;LS=0;RS=0;NM=99;AS=94;XS=97;PR=100;CL=4;MQ=60;BQ=33 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:98:52:46:32,27:0:0:99:94:97:100:4:60:33 -22 21991072 . T C 100 . 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a/test/aa/work_normal/work.0/region.bed b/test/aa/work_normal/work.0/region.bed deleted file mode 100644 index 1953509..0000000 --- a/test/aa/work_normal/work.0/region.bed +++ /dev/null @@ -1,24 +0,0 @@ -22 21172863 21172884 -22 21213050 21213071 -22 21330465 21330479 -22 21330598 21330619 -22 21330777 21330798 -22 21332112 21332133 -22 21334919 21334935 -22 21335249 21335270 -22 21337315 21337336 -22 21341673 21341689 -22 21377324 21377345 -22 21380056 21380075 -22 21380520 21380541 -22 21384506 21384589 -22 21386009 21386030 -22 21968712 21968728 -22 21982882 21982903 -22 21983250 21983271 -22 21983843 21983864 -22 21984280 21984293 -22 21988514 21988605 -22 21988606 21988613 -22 21989949 21989972 -22 21991062 21991083 diff --git a/test/aa/work_normal/work.0/scan.err b/test/aa/work_normal/work.0/scan.err deleted file mode 100644 index 8750005..0000000 --- a/test/aa/work_normal/work.0/scan.err +++ /dev/null @@ -1,3 +0,0 @@ -#../neusomatic/bin/scan_alignments --ref example/Homo_sapiens.GRCh37.75.dna.chromosome.22.fa -b normal.bam -L aa/work_tumor/candidates_region_0.bed --out_vcf_file aa/work_normal/work.0/candidates.vcf --out_count_file aa/work_normal/work.0/count.bed --window_size 2000 --min_af 0.2 --min_mapq 10 --max_depth 2000000.0 --calculate_qual_stat -#On region 22:? [21172864,21172886) -#Aligned read number: 40 diff --git a/test/aa/work_normal/work.0/scan.out b/test/aa/work_normal/work.0/scan.out deleted file mode 100644 index e69de29..0000000 diff --git a/test/aa/work_tumor/all_regions.bed b/test/aa/work_tumor/all_regions.bed deleted file mode 100644 index 0ae5c07..0000000 --- a/test/aa/work_tumor/all_regions.bed +++ /dev/null @@ -1,154 +0,0 @@ -22 21075520 21075691 -22 21079770 21079891 -22 21080006 21080098 -22 21080713 21080886 -22 21081535 21081746 -22 21081968 21082172 -22 21083623 21083879 -22 21083884 21084069 -22 21084179 21084373 -22 21096530 21096704 -22 21096882 21097089 -22 21098919 21099124 -22 21101885 21102011 -22 21104099 21104280 -22 21105576 21105751 -22 21105939 21106104 -22 21107165 21107401 -22 21115570 21115758 -22 21119009 21119215 -22 21119314 21119434 -22 21119438 21119558 -22 21119844 21119964 -22 21119969 21120089 -22 21133533 21134507 -22 21138215 21138416 -22 21138421 21138541 -22 21140210 21140226 -22 21140240 21140424 -22 21141104 21141301 -22 21147347 21147549 -22 21150412 21150628 -22 21152829 21153005 -22 21153309 21153528 -22 21153931 21154158 -22 21156164 21156284 -22 21156289 21156409 -22 21157393 21157585 -22 21158595 21158763 -22 21159313 21159517 -22 21161610 21161795 -22 21165178 21165388 -22 21167617 21167782 -22 21172701 21172821 -22 21172825 21172945 -22 21173930 21174213 -22 21174760 21174925 -22 21178571 21178740 -22 21188752 21188945 -22 21192891 21193062 -22 21212839 21212960 -22 21213032 21213161 -22 21213394 21213411 -22 21213422 21213514 -22 21213515 21213718 -22 21224656 21224864 -22 21235294 21235461 -22 21237646 21237865 -22 21241933 21242125 -22 21272233 21272601 -22 21288097 21288445 -22 21288446 21288566 -22 21303944 21304136 -22 21311502 21311526 -22 21311537 21311716 -22 21311727 21312025 -22 21322169 21322274 -22 21327533 21327825 -22 21328018 21328138 -22 21328143 21328263 -22 21328382 21328662 -22 21328782 21329068 -22 21329929 21330129 -22 21330465 21330646 -22 21330650 21331038 -22 21331049 21331353 -22 21332007 21332288 -22 21333490 21333683 -22 21333906 21334080 -22 21334243 21334452 -22 21334919 21335093 -22 21335137 21335351 -22 21336697 21336864 -22 21337304 21337410 -22 21337421 21337471 -22 21340075 21340083 -22 21340094 21340195 -22 21340198 21340318 -22 21341673 21341881 -22 21342224 21342444 -22 21343045 21343280 -22 21343816 21343950 -22 21344699 21344879 -22 21345974 21346172 -22 21346497 21346714 -22 21346725 21346732 -22 21347066 21347267 -22 21347869 21348039 -22 21348161 21348622 -22 21348848 21349032 -22 21349043 21349275 -22 21349548 21349752 -22 21349992 21350371 -22 21350951 21351634 -22 21354197 21354755 -22 21354923 21354965 -22 21354976 21355102 -22 21355238 21355411 -22 21355499 21355664 -22 21356015 21356235 -22 21356332 21356533 -22 21356751 21356927 -22 21357060 21357181 -22 21357232 21357353 -22 21376844 21376858 -22 21376869 21377061 -22 21377190 21377296 -22 21377307 21377376 -22 21377377 21377907 -22 21380056 21380338 -22 21380512 21380875 -22 21383404 21383745 -22 21383979 21384489 -22 21384494 21384614 -22 21385137 21385980 -22 21385991 21386086 -22 21388418 21388539 -22 21406469 21406637 -22 21407574 21407748 -22 21409356 21409534 -22 21411398 21411567 -22 21799211 21800037 -22 21800048 21800439 -22 21800450 21800736 -22 21800741 21800993 -22 21922024 21922145 -22 21947239 21947367 -22 21965108 21965208 -22 21965288 21965388 -22 21968712 21968933 -22 21982663 21983069 -22 21983213 21983333 -22 21983338 21983675 -22 21983678 21984200 -22 21984211 21984293 -22 21987203 21987323 -22 21987327 21987500 -22 21988354 21988605 -22 21988606 21989372 -22 21989375 21989710 -22 21989712 21989974 -22 21990774 21991374 -22 21996654 21996832 -22 21997188 21997393 -22 21998217 21998487 diff --git a/test/aa/work_tumor/candidates_region_0.bed b/test/aa/work_tumor/candidates_region_0.bed deleted file mode 100644 index 1953509..0000000 --- a/test/aa/work_tumor/candidates_region_0.bed +++ /dev/null @@ -1,24 +0,0 @@ -22 21172863 21172884 -22 21213050 21213071 -22 21330465 21330479 -22 21330598 21330619 -22 21330777 21330798 -22 21332112 21332133 -22 21334919 21334935 -22 21335249 21335270 -22 21337315 21337336 -22 21341673 21341689 -22 21377324 21377345 -22 21380056 21380075 -22 21380520 21380541 -22 21384506 21384589 -22 21386009 21386030 -22 21968712 21968728 -22 21982882 21982903 -22 21983250 21983271 -22 21983843 21983864 -22 21984280 21984293 -22 21988514 21988605 -22 21988606 21988613 -22 21989949 21989972 -22 21991062 21991083 diff --git a/test/aa/work_tumor/filtered_candidates.vcf b/test/aa/work_tumor/filtered_candidates.vcf deleted file mode 100644 index c467f92..0000000 --- a/test/aa/work_tumor/filtered_candidates.vcf +++ /dev/null @@ -1,29 +0,0 @@ -##fileformat=VCFv4.2 -#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT SAMPLE -22 21172873 . A C 100 . DP=85;RO=61;AO=24;ST=29,10;LS=0;RS=0;NM=99;AS=96;XS=85;PR=100;CL=0;MQ=60;BQ=37;AF=0.2824 GT:DP:RO:AO:AF 0/1:85:61:24:0.2824 -22 21213060 . C G 100 . DP=208;RO=160;AO=48;ST=90,26;LS=0;RS=0;NM=99;AS=94;XS=87;PR=100;CL=15;MQ=60;BQ=34;AF=0.2308 GT:DP:RO:AO:AF 0/1:208:160:48:0.2308 -22 21330465 . CCAT C 100 . DP=134;RO=105;AO=29;ST=77,19;LS=0;RS=0;NM=84;AS=77;XS=83;PR=97;CL=7;MQ=60;BQ=36;AF=0.2164 GT:DP:RO:AO:AF 0/1:134:105:29:0.2164 -22 21330608 . C T 100 . DP=271;RO=198;AO=73;ST=117,40;LS=0;RS=0;NM=99;AS=95;XS=85;PR=100;CL=3;MQ=60;BQ=32;AF=0.2694 GT:DP:RO:AO:AF 0/1:271:198:73:0.2694 -22 21330787 . C T 100 . DP=396;RO=306;AO=88;ST=147,37;LS=0;RS=0;NM=99;AS=95;XS=89;PR=99;CL=9;MQ=60;BQ=33;AF=0.2234 GT:DP:RO:AO:AF 0/1:396:306:88:0.2234 -22 21332122 . G A 100 . DP=285;RO=223;AO=62;ST=129,39;LS=0;RS=0;NM=99;AS=95;XS=85;PR=100;CL=5;MQ=60;BQ=33;AF=0.2175 GT:DP:RO:AO:AF 0/1:285:223:62:0.2175 -22 21334924 . G C 100 . DP=106;RO=83;AO=23;ST=62,16;LS=0;RS=0;NM=98;AS=94;XS=91;PR=100;CL=4;MQ=60;BQ=37;AF=0.217 GT:DP:RO:AO:AF 0/1:106:83:23:0.217 -22 21335259 . C A 100 . DP=249;RO=200;AO=49;ST=103,26;LS=0;RS=0;NM=99;AS=96;XS=85;PR=100;CL=0;MQ=60;BQ=32;AF=0.1968 GT:DP:RO:AO:AF 0/1:249:200:49:0.1968 -22 21337325 . G A 100 . DP=98;RO=76;AO=22;ST=43,14;LS=0;RS=0;NM=99;AS=94;XS=84;PR=100;CL=5;MQ=60;BQ=33;AF=0.2245 GT:DP:RO:AO:AF 0/1:98:76:22:0.2245 -22 21341676 . T A 100 . DP=21;RO=18;AO=3;ST=14,1;LS=0;RS=0;NM=97;AS=83;XS=72;PR=100;CL=33;MQ=60;BQ=25;AF=0.1429 GT:DP:RO:AO:AF 0/1:21:18:3:0.1429 -22 21341677 . C CA 100 . DP=23;RO=21;AO=2;ST=14,2;LS=0;RS=0;NM=94;AS=66;XS=71;PR=100;CL=50;MQ=60;BQ=34;AF=0.087 GT:DP:RO:AO:AF 0/1:23:21:2:0.087 -22 21341677 . CA C 100 . DP=24;RO=20;AO=4;ST=13,4;LS=0;RS=0;NM=98;AS=72;XS=64;PR=100;CL=25;MQ=58;BQ=32;AF=0.1667 GT:DP:RO:AO:AF 0/1:24:20:4:0.1667 -22 21377334 . C T 100 . DP=353;RO=279;AO=74;ST=152,36;LS=0;RS=0;NM=98;AS=90;XS=84;PR=100;CL=8;MQ=60;BQ=34;AF=0.2096 GT:DP:RO:AO:AF 0/1:353:279:74:0.2096 -22 21380064 . G A 100 . DP=206;RO=159;AO=47;ST=103,30;LS=0;RS=0;NM=99;AS=95;XS=92;PR=100;CL=19;MQ=60;BQ=34;AF=0.2282 GT:DP:RO:AO:AF 0/1:206:159:47:0.2282 -22 21380530 . G T 100 . DP=34;RO=32;AO=2;ST=23,1;LS=0;RS=0;NM=99;AS=92;XS=84;PR=100;CL=100;MQ=60;BQ=34;AF=0.0588 GT:DP:RO:AO:AF 0/1:34:32:2:0.0588 -22 21384516 . C T 100 . DP=95;RO=68;AO=27;ST=24,7;LS=0;RS=0;NM=99;AS=95;XS=86;PR=100;CL=11;MQ=60;BQ=35;AF=0.2842 GT:DP:RO:AO:AF 0/1:95:68:27:0.2842 -22 21384578 . C T 100 . DP=81;RO=58;AO=23;ST=25,9;LS=0;RS=0;NM=99;AS=94;XS=85;PR=100;CL=13;MQ=60;BQ=32;AF=0.284 GT:DP:RO:AO:AF 0/1:81:58:23:0.284 -22 21386019 . G A 100 . DP=156;RO=124;AO=32;ST=41,13;LS=0;RS=0;NM=99;AS=95;XS=87;PR=100;CL=0;MQ=60;BQ=33;AF=0.2051 GT:DP:RO:AO:AF 0/1:156:124:32:0.2051 -22 21968716 . CA C 100 . DP=109;RO=91;AO=17;ST=65,13;LS=0;RS=0;NM=98;AS=85;XS=70;PR=94;CL=35;MQ=61;BQ=31;AF=0.1574 GT:DP:RO:AO:AF 0/1:109:91:17:0.1574 -22 21982892 . C T 100 . DP=158;RO=113;AO=45;ST=58,23;LS=0;RS=0;NM=99;AS=96;XS=99;PR=100;CL=0;MQ=60;BQ=33;AF=0.2848 GT:DP:RO:AO:AF 0/1:158:113:45:0.2848 -22 21983260 . A G 100 . DP=118;RO=74;AO=44;ST=41,29;LS=0;RS=0;NM=99;AS=95;XS=99;PR=100;CL=0;MQ=60;BQ=33;AF=0.3729 GT:DP:RO:AO:AF 0/1:118:74:44:0.3729 -22 21983853 . G A 100 . DP=337;RO=249;AO=87;ST=81,35;LS=0;RS=0;NM=99;AS=95;XS=95;PR=100;CL=10;MQ=60;BQ=31;AF=0.2589 GT:DP:RO:AO:AF 0/1:337:249:87:0.2589 -22 21984290 . C A 100 . DP=8;RO=7;AO=1;ST=2,0;LS=0;RS=0;NM=99;AS=96;XS=85;PR=100;CL=0;MQ=60;BQ=36;AF=0.125 GT:DP:RO:AO:AF 0/1:8:7:1:0.125 -22 21988524 . C T 100 . DP=217;RO=167;AO=50;ST=95,31;LS=0;RS=0;NM=99;AS=93;XS=88;PR=98;CL=14;MQ=60;BQ=33;AF=0.2304 GT:DP:RO:AO:AF 0/1:217:167:50:0.2304 -22 21988602 . C T 100 . DP=203;RO=162;AO=41;ST=91,21;LS=0;RS=0;NM=99;AS=95;XS=98;PR=98;CL=5;MQ=60;BQ=34;AF=0.202 GT:DP:RO:AO:AF 0/1:203:162:41:0.202 -22 21989959 . AAG A 100 . DP=139;RO=107;AO=32;ST=33,11;LS=0;RS=0;NM=98;AS=93;XS=91;PR=100;CL=6;MQ=61;BQ=35;AF=0.2302 GT:DP:RO:AO:AF 0/1:139:107:32:0.2302 -22 21991072 . T C 100 . DP=109;RO=83;AO=26;ST=44,14;LS=0;RS=0;NM=99;AS=96;XS=86;PR=100;CL=0;MQ=60;BQ=32;AF=0.2385 GT:DP:RO:AO:AF 0/1:109:83:26:0.2385 diff --git a/test/aa/work_tumor/region_0.bed b/test/aa/work_tumor/region_0.bed deleted file mode 100644 index 82fa6f7..0000000 --- a/test/aa/work_tumor/region_0.bed +++ /dev/null @@ -1,154 +0,0 @@ -22 21075520 21075691 . . . -22 21079770 21079891 . . . -22 21080006 21080098 . . . -22 21080713 21080886 . . . -22 21081535 21081746 . . . -22 21081968 21082172 . . . -22 21083623 21083879 . . . -22 21083884 21084069 . . . -22 21084179 21084373 . . . -22 21096530 21096704 . . . -22 21096882 21097089 . . . -22 21098919 21099124 . . . -22 21101885 21102011 . . . -22 21104099 21104280 . . . -22 21105576 21105751 . . . -22 21105939 21106104 . . . -22 21107165 21107401 . . . -22 21115570 21115758 . . . -22 21119009 21119215 . . . -22 21119314 21119434 . . . -22 21119438 21119558 . . . -22 21119844 21119964 . . . -22 21119969 21120089 . . . -22 21133533 21134507 . . . -22 21138215 21138416 . . . -22 21138421 21138541 . . . -22 21140210 21140226 . . . -22 21140240 21140424 . . . -22 21141104 21141301 . . . -22 21147347 21147549 . . . -22 21150412 21150628 . . . -22 21152829 21153005 . . . -22 21153309 21153528 . . . -22 21153931 21154158 . . . -22 21156164 21156284 . . . -22 21156289 21156409 . . . -22 21157393 21157585 . . . -22 21158595 21158763 . . . -22 21159313 21159517 . . . -22 21161610 21161795 . . . -22 21165178 21165388 . . . -22 21167617 21167782 . . . -22 21172701 21172821 . . . -22 21172825 21172945 . . . -22 21173930 21174213 . . . -22 21174760 21174925 . . . -22 21178571 21178740 . . . -22 21188752 21188945 . . . -22 21192891 21193062 . . . -22 21212839 21212960 . . . -22 21213032 21213161 . . . -22 21213394 21213411 . . . -22 21213422 21213514 . . . -22 21213515 21213718 . . . -22 21224656 21224864 . . . -22 21235294 21235461 . . . -22 21237646 21237865 . . . -22 21241933 21242125 . . . -22 21272233 21272601 . . . -22 21288097 21288445 . . . -22 21288446 21288566 . . . -22 21303944 21304136 . . . -22 21311502 21311526 . . . -22 21311537 21311716 . . . -22 21311727 21312025 . . . -22 21322169 21322274 . . . -22 21327533 21327825 . . . -22 21328018 21328138 . . . -22 21328143 21328263 . . . -22 21328382 21328662 . . . -22 21328782 21329068 . . . -22 21329929 21330129 . . . -22 21330465 21330646 . . . -22 21330650 21331038 . . . -22 21331049 21331353 . . . -22 21332007 21332288 . . . -22 21333490 21333683 . . . -22 21333906 21334080 . . . -22 21334243 21334452 . . . -22 21334919 21335093 . . . -22 21335137 21335351 . . . -22 21336697 21336864 . . . -22 21337304 21337410 . . . -22 21337421 21337471 . . . -22 21340075 21340083 . . . -22 21340094 21340195 . . . -22 21340198 21340318 . . . -22 21341673 21341881 . . . -22 21342224 21342444 . . . -22 21343045 21343280 . . . -22 21343816 21343950 . . . -22 21344699 21344879 . . . -22 21345974 21346172 . . . -22 21346497 21346714 . . . -22 21346725 21346732 . . . -22 21347066 21347267 . . . -22 21347869 21348039 . . . -22 21348161 21348622 . . . -22 21348848 21349032 . . . -22 21349043 21349275 . . . -22 21349548 21349752 . . . -22 21349992 21350371 . . . -22 21350951 21351634 . . . -22 21354197 21354755 . . . -22 21354923 21354965 . . . -22 21354976 21355102 . . . -22 21355238 21355411 . . . -22 21355499 21355664 . . . -22 21356015 21356235 . . . -22 21356332 21356533 . . . -22 21356751 21356927 . . . -22 21357060 21357181 . . . -22 21357232 21357353 . . . -22 21376844 21376858 . . . -22 21376869 21377061 . . . -22 21377190 21377296 . . . -22 21377307 21377376 . . . -22 21377377 21377907 . . . -22 21380056 21380338 . . . -22 21380512 21380875 . . . -22 21383404 21383745 . . . -22 21383979 21384489 . . . -22 21384494 21384614 . . . -22 21385137 21385980 . . . -22 21385991 21386086 . . . -22 21388418 21388539 . . . -22 21406469 21406637 . . . -22 21407574 21407748 . . . -22 21409356 21409534 . . . -22 21411398 21411567 . . . -22 21799211 21800037 . . . -22 21800048 21800439 . . . -22 21800450 21800736 . . . -22 21800741 21800993 . . . -22 21922024 21922145 . . . -22 21947239 21947367 . . . -22 21965108 21965208 . . . -22 21965288 21965388 . . . -22 21968712 21968933 . . . -22 21982663 21983069 . . . -22 21983213 21983333 . . . -22 21983338 21983675 . . . -22 21983678 21984200 . . . -22 21984211 21984293 . . . -22 21987203 21987323 . . . -22 21987327 21987500 . . . -22 21988354 21988605 . . . -22 21988606 21989372 . . . -22 21989375 21989710 . . . -22 21989712 21989974 . . . -22 21990774 21991374 . . . -22 21996654 21996832 . . . -22 21997188 21997393 . . . -22 21998217 21998487 . . . diff --git a/test/aa/work_tumor/work.0/candidates.vcf b/test/aa/work_tumor/work.0/candidates.vcf deleted file mode 100644 index 10d8136..0000000 --- a/test/aa/work_tumor/work.0/candidates.vcf +++ /dev/null @@ -1,43 +0,0 @@ -##fileformat=VCFv4.2 -##source=VarCal -##reference=example/Homo_sapiens.GRCh37.75.dna.chromosome.22.fa -##INFO= -#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT -22 21080040 . T G 100 . AF=0.095238;DP=21;RO=19;AO=2;ST=7,1;LS=0;RS=0;NM=99;AS=93;XS=68;PR=100;CL=0;MQ=60;BQ=12 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:21:19:2:7,1:0:0:99:93:68:100:0:60:12 -22 21172873 . A C 100 . AF=0.282353;DP=85;RO=61;AO=24;ST=29,10;LS=0;RS=0;NM=99;AS=96;XS=85;PR=100;CL=0;MQ=60;BQ=37 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:85:61:24:29,10:0:0:99:96:85:100:0:60:37 -22 21213060 . C G 100 . AF=0.230769;DP=208;RO=160;AO=48;ST=90,26;LS=0;RS=0;NM=99;AS=94;XS=87;PR=100;CL=15;MQ=60;BQ=34 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:208:160:48:90,26:0:0:99:94:87:100:15:60:34 -22 21328480 . T G 100 . AF=0.076923;DP=91;RO=84;AO=7;ST=51,0;LS=0;RS=0;NM=97;AS=81;XS=89;PR=100;CL=29;MQ=60;BQ=13 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:91:84:7:51,0:0:0:97:81:89:100:29:60:13 -22 21330466 . C N 100 . AF=0.218045;DP=133;RO=104;AO=29;ST=77,19;LS=0;RS=0;NM=84;AS=77;XS=83;PR=97;CL=7;MQ=60;BQ=36 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:133:104:29:77,19:0:0:84:77:83:97:7:60:36 -22 21330467 . A N 100 . AF=0.216418;DP=134;RO=105;AO=29;ST=77,19;LS=0;RS=0;NM=84;AS=77;XS=83;PR=97;CL=7;MQ=60;BQ=36 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:134:105:29:77,19:0:0:84:77:83:97:7:60:36 -22 21330468 . T N 100 . AF=0.216418;DP=134;RO=105;AO=29;ST=77,19;LS=0;RS=0;NM=84;AS=77;XS=83;PR=97;CL=7;MQ=60;BQ=36 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:134:105:29:77,19:0:0:84:77:83:97:7:60:36 -22 21330608 . C T 100 . AF=0.269373;DP=271;RO=198;AO=73;ST=117,40;LS=0;RS=0;NM=99;AS=95;XS=85;PR=100;CL=3;MQ=60;BQ=32 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:271:198:73:117,40:0:0:99:95:85:100:3:60:32 -22 21330787 . C T 100 . AF=0.223350;DP=396;RO=306;AO=88;ST=147,37;LS=0;RS=0;NM=99;AS=95;XS=89;PR=99;CL=9;MQ=60;BQ=33 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:396:306:88:147,37:0:0:99:95:89:99:9:60:33 -22 21332122 . G A 100 . AF=0.217544;DP=285;RO=223;AO=62;ST=129,39;LS=0;RS=0;NM=99;AS=95;XS=85;PR=100;CL=5;MQ=60;BQ=33 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:285:223:62:129,39:0:0:99:95:85:100:5:60:33 -22 21333636 . T G 100 . AF=0.051724;DP=58;RO=55;AO=3;ST=21,0;LS=0;RS=0;NM=92;AS=60;XS=79;PR=100;CL=0;MQ=60;BQ=12 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:58:55:3:21,0:0:0:92:60:79:100:0:60:12 -22 21333662 . A C 100 . AF=0.057692;DP=52;RO=49;AO=3;ST=19,0;LS=0;RS=0;NM=94;AS=63;XS=79;PR=100;CL=33;MQ=60;BQ=14 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:52:49:3:19,0:0:0:94:63:79:100:33:60:14 -22 21333684 . C G 100 . AF=0.151515;DP=33;RO=28;AO=5;ST=12,0;LS=0;RS=0;NM=94;AS=57;XS=81;PR=100;CL=60;MQ=60;BQ=11 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:33:28:5:12,0:0:0:94:57:81:100:60:60:11 -22 21334924 . G C 100 . AF=0.216981;DP=106;RO=83;AO=23;ST=62,16;LS=0;RS=0;NM=98;AS=94;XS=91;PR=100;CL=4;MQ=60;BQ=37 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:106:83:23:62,16:0:0:98:94:91:100:4:60:37 -22 21335259 . C A 100 . AF=0.196787;DP=249;RO=200;AO=49;ST=103,26;LS=0;RS=0;NM=99;AS=96;XS=85;PR=100;CL=0;MQ=60;BQ=32 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:249:200:49:103,26:0:0:99:96:85:100:0:60:32 -22 21337325 . G A 100 . AF=0.224490;DP=98;RO=76;AO=22;ST=43,14;LS=0;RS=0;NM=99;AS=94;XS=84;PR=100;CL=5;MQ=60;BQ=33 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:98:76:22:43,14:0:0:99:94:84:100:5:60:33 -22 21341676 . T A 100 . AF=0.142857;DP=21;RO=18;AO=3;ST=14,1;LS=0;RS=0;NM=97;AS=83;XS=72;PR=100;CL=33;MQ=60;BQ=25 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:21:18:3:14,1:0:0:97:83:72:100:33:60:25 -22 21341677 . C A 100 . AF=0.181818;DP=23;RO=18;AO=4;ST=16,0;LS=0;RS=0;NM=97;AS=79;XS=73;PR=100;CL=50;MQ=60;BQ=17 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:23:18:4:16,0:0:0:97:79:73:100:50:60:17 -22 21341678 . N A 100 . AF=0.086957;DP=23;RO=21;AO=2;ST=14,2;LS=0;RS=0;NM=94;AS=66;XS=71;PR=100;CL=50;MQ=60;BQ=34 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:23:21:2:14,2:0:0:94:66:71:100:50:60:34 -22 21341678 . A N 100 . AF=0.166667;DP=24;RO=20;AO=4;ST=13,4;LS=0;RS=0;NM=98;AS=72;XS=64;PR=100;CL=25;MQ=58;BQ=32 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:24:20:4:13,4:0:0:98:72:64:100:25:58:32 -22 21377334 . C T 100 . AF=0.209632;DP=353;RO=279;AO=74;ST=152,36;LS=0;RS=0;NM=98;AS=90;XS=84;PR=100;CL=8;MQ=60;BQ=34 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:353:279:74:152,36:0:0:98:90:84:100:8:60:34 -22 21380064 . G A 100 . AF=0.228155;DP=206;RO=159;AO=47;ST=103,30;LS=0;RS=0;NM=99;AS=95;XS=92;PR=100;CL=19;MQ=60;BQ=34 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:206:159:47:103,30:0:0:99:95:92:100:19:60:34 -22 21380530 . G T 100 . AF=0.058824;DP=34;RO=32;AO=2;ST=23,1;LS=0;RS=0;NM=99;AS=92;XS=84;PR=100;CL=100;MQ=60;BQ=34 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:34:32:2:23,1:0:0:99:92:84:100:100:60:34 -22 21384516 . C T 100 . AF=0.284211;DP=95;RO=68;AO=27;ST=24,7;LS=0;RS=0;NM=99;AS=95;XS=86;PR=100;CL=11;MQ=60;BQ=35 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:95:68:27:24,7:0:0:99:95:86:100:11:60:35 -22 21384578 . C T 100 . AF=0.283951;DP=81;RO=58;AO=23;ST=25,9;LS=0;RS=0;NM=99;AS=94;XS=85;PR=100;CL=13;MQ=60;BQ=32 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:81:58:23:25,9:0:0:99:94:85:100:13:60:32 -22 21386019 . G A 100 . AF=0.205128;DP=156;RO=124;AO=32;ST=41,13;LS=0;RS=0;NM=99;AS=95;XS=87;PR=100;CL=0;MQ=60;BQ=33 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:156:124:32:41,13:0:0:99:95:87:100:0:60:33 -22 21968717 . A N 100 . AF=0.157407;DP=109;RO=91;AO=17;ST=65,13;LS=0;RS=0;NM=98;AS=85;XS=70;PR=94;CL=35;MQ=61;BQ=31 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:109:91:17:65,13:0:0:98:85:70:94:35:61:31 -22 21982892 . C T 100 . AF=0.284810;DP=158;RO=113;AO=45;ST=58,23;LS=0;RS=0;NM=99;AS=96;XS=99;PR=100;CL=0;MQ=60;BQ=33 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:158:113:45:58,23:0:0:99:96:99:100:0:60:33 -22 21983260 . A G 100 . AF=0.372881;DP=118;RO=74;AO=44;ST=41,29;LS=0;RS=0;NM=99;AS=95;XS=99;PR=100;CL=0;MQ=60;BQ=33 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:118:74:44:41,29:0:0:99:95:99:100:0:60:33 -22 21983853 . G A 100 . AF=0.258929;DP=337;RO=249;AO=87;ST=81,35;LS=0;RS=0;NM=99;AS=95;XS=95;PR=100;CL=10;MQ=60;BQ=31 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:337:249:87:81,35:0:0:99:95:95:100:10:60:31 -22 21984227 . A C 100 . AF=0.071429;DP=14;RO=13;AO=1;ST=2,1;LS=0;RS=0;NM=98;AS=95;XS=100;PR=100;CL=0;MQ=60;BQ=9 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:14:13:1:2,1:0:0:98:95:100:100:0:60:9 -22 21984248 . G C 100 . AF=0.090909;DP=11;RO=10;AO=1;ST=1,1;LS=0;RS=0;NM=98;AS=95;XS=100;PR=100;CL=0;MQ=60;BQ=9 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:11:10:1:1,1:0:0:98:95:100:100:0:60:9 -22 21984290 . C A 100 . AF=0.125000;DP=8;RO=7;AO=1;ST=2,0;LS=0;RS=0;NM=99;AS=96;XS=85;PR=100;CL=0;MQ=60;BQ=36 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:8:7:1:2,0:0:0:99:96:85:100:0:60:36 -22 21988524 . C T 100 . AF=0.230415;DP=217;RO=167;AO=50;ST=95,31;LS=0;RS=0;NM=99;AS=93;XS=88;PR=98;CL=14;MQ=60;BQ=33 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:217:167:50:95,31:0:0:99:93:88:98:14:60:33 -22 21988602 . C T 100 . AF=0.201970;DP=203;RO=162;AO=41;ST=91,21;LS=0;RS=0;NM=99;AS=95;XS=98;PR=98;CL=5;MQ=60;BQ=34 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:203:162:41:91,21:0:0:99:95:98:98:5:60:34 -22 21989960 . A N 100 . AF=0.230216;DP=139;RO=107;AO=32;ST=33,11;LS=0;RS=0;NM=98;AS=93;XS=91;PR=100;CL=6;MQ=61;BQ=35 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:139:107:32:33,11:0:0:98:93:91:100:6:61:35 -22 21989961 . G N 100 . AF=0.231884;DP=138;RO=106;AO=32;ST=33,11;LS=0;RS=0;NM=98;AS=93;XS=91;PR=100;CL=6;MQ=61;BQ=35 GT:DP:RO:AO:ST:LS:RS:NM:AS:XS:PR:CL:MQ:BQ 0/1:138:106:32:33,11:0:0:98:93:91:100:6:61:35 -22 21991072 . T C 100 . 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z150w}L?BP96Gmznuw2jbXR0*m=q(*}p*+%4d8Bp2loI=)QSlS4o~q#P;4weL1KwWB z(WFZ)8{w9ujBWTOGIU$t)}6sB$LAU)g5km*PFYG%1FO(uLPNg-K_RA zja^yjdKLvPUx>@yFp+fn;s`d5E=nUkl}1`O$|EI{`!*iX_);8is)y<hi_DLv<^pObMj`m5v8!yyDdTJhN?dS@EAL0RHM`CO{ zI_7fmXLzd6INEj;&T{bt&j)Vs7c6 z_zM4^7}7^h(za&Q#l~qDqK^+D#`Xh#rlyhBjP@cl>u8j|GG5^4NKx$Rui8(1P5<@( nVVxlfkn{imABzYC000000RIL6LPG)o8vp|U0000000000Rmd_P diff --git a/test/aa/work_tumor/work.0/count.bed.gz.tbi b/test/aa/work_tumor/work.0/count.bed.gz.tbi deleted file mode 100644 index a9524444d81a245c451c3f55b6ef83e311f8d319..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 424 zcmb2|=3rp}f&Xj_PR>jW-HdN%ZS-bJlsI;N^JbAn2Ns&KOLr|exOAhC6xX72YnCXk zUAADaqDMw&hghpq<_hUW2c|7h5SZ?ik#XW5o4VtymRY~z_I+3Vosq>oUw)4L{O4cZ zJ-DVWIsfBN9p0N^?eVgK?_N~vq_s{>EB^JxTz=w&Upa68YpngrSgZMPv%tv-;yb3t z^qSgkI=%M^_x2cd^&Qjy2>9d#$32Pr^z`HjUCWbupA@Z_xafK8qqw34rTd-Zp2cnY zzNgeJ?qS>~-}pU#euers?JoEg+s|C*SE{*xZ&;Y@Q=aeArgOb-{;P4QoLha;d7ALs zW%mp9e}257JJEdS^cuSz*Hp}N{@OjUJoEc>?fu-p_4WCNw=aLYSN3yPk*%fu!w7Hy zVIf{9oXK6j^RCJEna{pmx)avrp3U06Y{lbn_rp(K@1Ab?>2itc^Z%*8GxD@kt$)Vr vN}t^ywQX+ox^Ibrw@=Qmdg5YYBbHO$AFt_tR$N?%fk7TEE~FWl;g$jbI=H_% diff --git a/test/aa/work_tumor/work.0/filtered_candidates.vcf b/test/aa/work_tumor/work.0/filtered_candidates.vcf deleted file mode 100644 index c467f92..0000000 --- a/test/aa/work_tumor/work.0/filtered_candidates.vcf +++ /dev/null @@ -1,29 +0,0 @@ -##fileformat=VCFv4.2 -#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT SAMPLE -22 21172873 . A C 100 . DP=85;RO=61;AO=24;ST=29,10;LS=0;RS=0;NM=99;AS=96;XS=85;PR=100;CL=0;MQ=60;BQ=37;AF=0.2824 GT:DP:RO:AO:AF 0/1:85:61:24:0.2824 -22 21213060 . C G 100 . DP=208;RO=160;AO=48;ST=90,26;LS=0;RS=0;NM=99;AS=94;XS=87;PR=100;CL=15;MQ=60;BQ=34;AF=0.2308 GT:DP:RO:AO:AF 0/1:208:160:48:0.2308 -22 21330465 . CCAT C 100 . DP=134;RO=105;AO=29;ST=77,19;LS=0;RS=0;NM=84;AS=77;XS=83;PR=97;CL=7;MQ=60;BQ=36;AF=0.2164 GT:DP:RO:AO:AF 0/1:134:105:29:0.2164 -22 21330608 . C T 100 . DP=271;RO=198;AO=73;ST=117,40;LS=0;RS=0;NM=99;AS=95;XS=85;PR=100;CL=3;MQ=60;BQ=32;AF=0.2694 GT:DP:RO:AO:AF 0/1:271:198:73:0.2694 -22 21330787 . C T 100 . DP=396;RO=306;AO=88;ST=147,37;LS=0;RS=0;NM=99;AS=95;XS=89;PR=99;CL=9;MQ=60;BQ=33;AF=0.2234 GT:DP:RO:AO:AF 0/1:396:306:88:0.2234 -22 21332122 . G A 100 . DP=285;RO=223;AO=62;ST=129,39;LS=0;RS=0;NM=99;AS=95;XS=85;PR=100;CL=5;MQ=60;BQ=33;AF=0.2175 GT:DP:RO:AO:AF 0/1:285:223:62:0.2175 -22 21334924 . G C 100 . DP=106;RO=83;AO=23;ST=62,16;LS=0;RS=0;NM=98;AS=94;XS=91;PR=100;CL=4;MQ=60;BQ=37;AF=0.217 GT:DP:RO:AO:AF 0/1:106:83:23:0.217 -22 21335259 . C A 100 . DP=249;RO=200;AO=49;ST=103,26;LS=0;RS=0;NM=99;AS=96;XS=85;PR=100;CL=0;MQ=60;BQ=32;AF=0.1968 GT:DP:RO:AO:AF 0/1:249:200:49:0.1968 -22 21337325 . G A 100 . DP=98;RO=76;AO=22;ST=43,14;LS=0;RS=0;NM=99;AS=94;XS=84;PR=100;CL=5;MQ=60;BQ=33;AF=0.2245 GT:DP:RO:AO:AF 0/1:98:76:22:0.2245 -22 21341676 . T A 100 . DP=21;RO=18;AO=3;ST=14,1;LS=0;RS=0;NM=97;AS=83;XS=72;PR=100;CL=33;MQ=60;BQ=25;AF=0.1429 GT:DP:RO:AO:AF 0/1:21:18:3:0.1429 -22 21341677 . C CA 100 . DP=23;RO=21;AO=2;ST=14,2;LS=0;RS=0;NM=94;AS=66;XS=71;PR=100;CL=50;MQ=60;BQ=34;AF=0.087 GT:DP:RO:AO:AF 0/1:23:21:2:0.087 -22 21341677 . CA C 100 . DP=24;RO=20;AO=4;ST=13,4;LS=0;RS=0;NM=98;AS=72;XS=64;PR=100;CL=25;MQ=58;BQ=32;AF=0.1667 GT:DP:RO:AO:AF 0/1:24:20:4:0.1667 -22 21377334 . C T 100 . DP=353;RO=279;AO=74;ST=152,36;LS=0;RS=0;NM=98;AS=90;XS=84;PR=100;CL=8;MQ=60;BQ=34;AF=0.2096 GT:DP:RO:AO:AF 0/1:353:279:74:0.2096 -22 21380064 . G A 100 . DP=206;RO=159;AO=47;ST=103,30;LS=0;RS=0;NM=99;AS=95;XS=92;PR=100;CL=19;MQ=60;BQ=34;AF=0.2282 GT:DP:RO:AO:AF 0/1:206:159:47:0.2282 -22 21380530 . G T 100 . DP=34;RO=32;AO=2;ST=23,1;LS=0;RS=0;NM=99;AS=92;XS=84;PR=100;CL=100;MQ=60;BQ=34;AF=0.0588 GT:DP:RO:AO:AF 0/1:34:32:2:0.0588 -22 21384516 . C T 100 . DP=95;RO=68;AO=27;ST=24,7;LS=0;RS=0;NM=99;AS=95;XS=86;PR=100;CL=11;MQ=60;BQ=35;AF=0.2842 GT:DP:RO:AO:AF 0/1:95:68:27:0.2842 -22 21384578 . C T 100 . DP=81;RO=58;AO=23;ST=25,9;LS=0;RS=0;NM=99;AS=94;XS=85;PR=100;CL=13;MQ=60;BQ=32;AF=0.284 GT:DP:RO:AO:AF 0/1:81:58:23:0.284 -22 21386019 . G A 100 . DP=156;RO=124;AO=32;ST=41,13;LS=0;RS=0;NM=99;AS=95;XS=87;PR=100;CL=0;MQ=60;BQ=33;AF=0.2051 GT:DP:RO:AO:AF 0/1:156:124:32:0.2051 -22 21968716 . CA C 100 . DP=109;RO=91;AO=17;ST=65,13;LS=0;RS=0;NM=98;AS=85;XS=70;PR=94;CL=35;MQ=61;BQ=31;AF=0.1574 GT:DP:RO:AO:AF 0/1:109:91:17:0.1574 -22 21982892 . C T 100 . DP=158;RO=113;AO=45;ST=58,23;LS=0;RS=0;NM=99;AS=96;XS=99;PR=100;CL=0;MQ=60;BQ=33;AF=0.2848 GT:DP:RO:AO:AF 0/1:158:113:45:0.2848 -22 21983260 . A G 100 . DP=118;RO=74;AO=44;ST=41,29;LS=0;RS=0;NM=99;AS=95;XS=99;PR=100;CL=0;MQ=60;BQ=33;AF=0.3729 GT:DP:RO:AO:AF 0/1:118:74:44:0.3729 -22 21983853 . G A 100 . DP=337;RO=249;AO=87;ST=81,35;LS=0;RS=0;NM=99;AS=95;XS=95;PR=100;CL=10;MQ=60;BQ=31;AF=0.2589 GT:DP:RO:AO:AF 0/1:337:249:87:0.2589 -22 21984290 . C A 100 . DP=8;RO=7;AO=1;ST=2,0;LS=0;RS=0;NM=99;AS=96;XS=85;PR=100;CL=0;MQ=60;BQ=36;AF=0.125 GT:DP:RO:AO:AF 0/1:8:7:1:0.125 -22 21988524 . C T 100 . DP=217;RO=167;AO=50;ST=95,31;LS=0;RS=0;NM=99;AS=93;XS=88;PR=98;CL=14;MQ=60;BQ=33;AF=0.2304 GT:DP:RO:AO:AF 0/1:217:167:50:0.2304 -22 21988602 . C T 100 . DP=203;RO=162;AO=41;ST=91,21;LS=0;RS=0;NM=99;AS=95;XS=98;PR=98;CL=5;MQ=60;BQ=34;AF=0.202 GT:DP:RO:AO:AF 0/1:203:162:41:0.202 -22 21989959 . AAG A 100 . DP=139;RO=107;AO=32;ST=33,11;LS=0;RS=0;NM=98;AS=93;XS=91;PR=100;CL=6;MQ=61;BQ=35;AF=0.2302 GT:DP:RO:AO:AF 0/1:139:107:32:0.2302 -22 21991072 . T C 100 . DP=109;RO=83;AO=26;ST=44,14;LS=0;RS=0;NM=99;AS=96;XS=86;PR=100;CL=0;MQ=60;BQ=32;AF=0.2385 GT:DP:RO:AO:AF 0/1:109:83:26:0.2385 diff --git a/test/aa/work_tumor/work.0/region.bed b/test/aa/work_tumor/work.0/region.bed deleted file mode 100644 index 82fa6f7..0000000 --- a/test/aa/work_tumor/work.0/region.bed +++ /dev/null @@ -1,154 +0,0 @@ -22 21075520 21075691 . . . -22 21079770 21079891 . . . -22 21080006 21080098 . . . -22 21080713 21080886 . . . -22 21081535 21081746 . . . -22 21081968 21082172 . . . -22 21083623 21083879 . . . -22 21083884 21084069 . . . -22 21084179 21084373 . . . -22 21096530 21096704 . . . -22 21096882 21097089 . . . -22 21098919 21099124 . . . -22 21101885 21102011 . . . -22 21104099 21104280 . . . -22 21105576 21105751 . . . -22 21105939 21106104 . . . -22 21107165 21107401 . . . -22 21115570 21115758 . . . -22 21119009 21119215 . . . -22 21119314 21119434 . . . -22 21119438 21119558 . . . -22 21119844 21119964 . . . -22 21119969 21120089 . . . -22 21133533 21134507 . . . -22 21138215 21138416 . . . -22 21138421 21138541 . . . -22 21140210 21140226 . . . -22 21140240 21140424 . . . -22 21141104 21141301 . . . -22 21147347 21147549 . . . -22 21150412 21150628 . . . -22 21152829 21153005 . . . -22 21153309 21153528 . . . -22 21153931 21154158 . . . -22 21156164 21156284 . . . -22 21156289 21156409 . . . -22 21157393 21157585 . . . -22 21158595 21158763 . . . -22 21159313 21159517 . . . -22 21161610 21161795 . . . -22 21165178 21165388 . . . -22 21167617 21167782 . . . -22 21172701 21172821 . . . -22 21172825 21172945 . . . -22 21173930 21174213 . . . -22 21174760 21174925 . . . -22 21178571 21178740 . . . -22 21188752 21188945 . . . -22 21192891 21193062 . . . -22 21212839 21212960 . . . -22 21213032 21213161 . . . -22 21213394 21213411 . . . -22 21213422 21213514 . . . -22 21213515 21213718 . . . -22 21224656 21224864 . . . -22 21235294 21235461 . . . -22 21237646 21237865 . . . -22 21241933 21242125 . . . -22 21272233 21272601 . . . -22 21288097 21288445 . . . -22 21288446 21288566 . . . -22 21303944 21304136 . . . -22 21311502 21311526 . . . -22 21311537 21311716 . . . -22 21311727 21312025 . . . -22 21322169 21322274 . . . -22 21327533 21327825 . . . -22 21328018 21328138 . . . -22 21328143 21328263 . . . -22 21328382 21328662 . . . -22 21328782 21329068 . . . -22 21329929 21330129 . . . -22 21330465 21330646 . . . -22 21330650 21331038 . . . -22 21331049 21331353 . . . -22 21332007 21332288 . . . -22 21333490 21333683 . . . -22 21333906 21334080 . . . -22 21334243 21334452 . . . -22 21334919 21335093 . . . -22 21335137 21335351 . . . -22 21336697 21336864 . . . -22 21337304 21337410 . . . -22 21337421 21337471 . . . -22 21340075 21340083 . . . -22 21340094 21340195 . . . -22 21340198 21340318 . . . -22 21341673 21341881 . . . -22 21342224 21342444 . . . -22 21343045 21343280 . . . -22 21343816 21343950 . . . -22 21344699 21344879 . . . -22 21345974 21346172 . . . -22 21346497 21346714 . . . -22 21346725 21346732 . . . -22 21347066 21347267 . . . -22 21347869 21348039 . . . -22 21348161 21348622 . . . -22 21348848 21349032 . . . -22 21349043 21349275 . . . -22 21349548 21349752 . . . -22 21349992 21350371 . . . -22 21350951 21351634 . . . -22 21354197 21354755 . . . -22 21354923 21354965 . . . -22 21354976 21355102 . . . -22 21355238 21355411 . . . -22 21355499 21355664 . . . -22 21356015 21356235 . . . -22 21356332 21356533 . . . -22 21356751 21356927 . . . -22 21357060 21357181 . . . -22 21357232 21357353 . . . -22 21376844 21376858 . . . -22 21376869 21377061 . . . -22 21377190 21377296 . . . -22 21377307 21377376 . . . -22 21377377 21377907 . . . -22 21380056 21380338 . . . -22 21380512 21380875 . . . -22 21383404 21383745 . . . -22 21383979 21384489 . . . -22 21384494 21384614 . . . -22 21385137 21385980 . . . -22 21385991 21386086 . . . -22 21388418 21388539 . . . -22 21406469 21406637 . . . -22 21407574 21407748 . . . -22 21409356 21409534 . . . -22 21411398 21411567 . . . -22 21799211 21800037 . . . -22 21800048 21800439 . . . -22 21800450 21800736 . . . -22 21800741 21800993 . . . -22 21922024 21922145 . . . -22 21947239 21947367 . . . -22 21965108 21965208 . . . -22 21965288 21965388 . . . -22 21968712 21968933 . . . -22 21982663 21983069 . . . -22 21983213 21983333 . . . -22 21983338 21983675 . . . -22 21983678 21984200 . . . -22 21984211 21984293 . . . -22 21987203 21987323 . . . -22 21987327 21987500 . . . -22 21988354 21988605 . . . -22 21988606 21989372 . . . -22 21989375 21989710 . . . -22 21989712 21989974 . . . -22 21990774 21991374 . . . -22 21996654 21996832 . . . -22 21997188 21997393 . . . -22 21998217 21998487 . . . diff --git a/test/aa/work_tumor/work.0/scan.err b/test/aa/work_tumor/work.0/scan.err deleted file mode 100644 index 1c7a731..0000000 --- a/test/aa/work_tumor/work.0/scan.err +++ /dev/null @@ -1,5 +0,0 @@ -#../neusomatic/bin/scan_alignments --ref example/Homo_sapiens.GRCh37.75.dna.chromosome.22.fa -b tumor.bam -L aa/work_tumor/region_0.bed --out_vcf_file aa/work_tumor/work.0/candidates.vcf --out_count_file aa/work_tumor/work.0/count.bed --window_size 2000 --min_af 0.05 --min_mapq 10 --max_depth 2000000.0 --calculate_qual_stat -#On region 22:? [21075521,21075693) -#Aligned read number: 177 -#On region 22:? [21349549,21349754) -#Aligned read number: 423 diff --git a/test/aa/work_tumor/work.0/scan.out b/test/aa/work_tumor/work.0/scan.out deleted file mode 100644 index e69de29..0000000 diff --git a/test/t.vcf b/test/t.vcf deleted file mode 100644 index ed4d78a..0000000 --- a/test/t.vcf +++ /dev/null @@ -1,24 +0,0 @@ -##fileformat=VCFv4.2 -##NeuSomatic Version=0.2.1 -##INFO= -##INFO= -##INFO= -##INFO= -##INFO= -##FILTER= -##FILTER= -##FILTER= -##FORMAT= -##FORMAT= -##FORMAT= -##FORMAT= -##FORMAT= -#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT SAMPLE -22 21330787 . c t 26.9917 PASS SCORE=0.9980;DP=396;RO=306;AO=88;AF=0.2234 GT:DP:RO:AO:AF 0/1:396:306:88:0.2234 -22 21332122 . G A 28.5402 PASS SCORE=0.9986;DP=285;RO=223;AO=62;AF=0.2175 GT:DP:RO:AO:AF 0/1:285:223:62:0.2175 -22 21334924 . G C 17.5639 PASS SCORE=0.9825;DP=106;RO=83;AO=23;AF=0.217 GT:DP:RO:AO:AF 0/1:106:83:23:0.217 -22 21335259 . C A 19.7149 PASS SCORE=0.9893;DP=249;RO=200;AO=49;AF=0.1968 GT:DP:RO:AO:AF 0/1:249:200:49:0.1968 -22 21384516 . C T 27.6969 PASS SCORE=0.9983;DP=95;RO=68;AO=27;AF=0.2842 GT:DP:RO:AO:AF 0/1:95:68:27:0.2842 -22 21982892 . C T 21.5561 PASS SCORE=0.9930;DP=158;RO=113;AO=45;AF=0.2848 GT:DP:RO:AO:AF 0/1:158:113:45:0.2848 -22 21983260 . A G 31.5494 PASS SCORE=0.9993;DP=118;RO=74;AO=44;AF=0.3729 GT:DP:RO:AO:AF 0/1:118:74:44:0.3729 -22 21989959 . AAG A 33.0106 PASS SCORE=0.9995;DP=139;RO=107;AO=32;AF=0.2302 GT:DP:RO:AO:AF 0/1:139:107:32:0.2302 From 0c7783032682f37886ed0deb70ce3c615151ffdd Mon Sep 17 00:00:00 2001 From: Sahraeian Date: Tue, 28 Apr 2020 13:32:44 -0700 Subject: [PATCH 12/15] few fixes --- neusomatic/python/filter_candidates.py | 2 +- neusomatic/python/long_read_indelrealign.py | 3 +++ 2 files changed, 4 insertions(+), 1 deletion(-) diff --git a/neusomatic/python/filter_candidates.py b/neusomatic/python/filter_candidates.py index 23f9763..39a1fdf 100755 --- a/neusomatic/python/filter_candidates.py +++ b/neusomatic/python/filter_candidates.py @@ -27,7 +27,7 @@ def filter_candidates(candidate_record): "---------------------Filter Candidates---------------------") if dbsnp: - if dbsnp[-6:] != "vcf.gz": + if not dbsnp.endswith("vcf.gz"): thread_logger.error("Aborting!") raise Exception( "The dbSNP file should be a tabix indexed file with .vcf.gz format") diff --git a/neusomatic/python/long_read_indelrealign.py b/neusomatic/python/long_read_indelrealign.py index 3f91364..bc62cca 100755 --- a/neusomatic/python/long_read_indelrealign.py +++ b/neusomatic/python/long_read_indelrealign.py @@ -859,6 +859,9 @@ def do_realign(region, info_file, max_realign_dp, thr_realign=0.0135): def find_var(out_fasta_file, snp_min_af, del_min_af, ins_min_af, scale_maf, simplify): + # Find variants from MSA: + # In each column the AF is calculated + # The low AF vars in each column are discarded and the variant is extracted logger = logging.getLogger(find_var.__name__) records = SeqIO.to_dict(SeqIO.parse(out_fasta_file, "fasta")) if set(map(int, records.keys())) ^ set(range(len(records))): From 804288ab8bacf077d11fa0a95f1ac72775479369 Mon Sep 17 00:00:00 2001 From: Sahraeian Date: Wed, 29 Apr 2020 00:14:39 -0700 Subject: [PATCH 13/15] fix postporcess --- neusomatic/python/postprocess.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/neusomatic/python/postprocess.py b/neusomatic/python/postprocess.py index 6df0a17..34ad405 100755 --- a/neusomatic/python/postprocess.py +++ b/neusomatic/python/postprocess.py @@ -21,7 +21,7 @@ from extract_postprocess_targets import extract_postprocess_targets from merge_post_vcfs import merge_post_vcfs from resolve_variants import resolve_variants -from utils import concatenate_files, get_chromosomes_order, bedtools_window, skip_empty +from utils import concatenate_files, get_chromosomes_order, bedtools_window, skip_empty, run_bedtools_cmd from long_read_indelrealign import long_read_indelrealign from resolve_scores import resolve_scores from _version import __version__ From d8b7c3cb79c944c4c691f02b08b30557c47f0465 Mon Sep 17 00:00:00 2001 From: Sahraeian Date: Wed, 29 Apr 2020 02:08:34 -0700 Subject: [PATCH 14/15] logging fix --- neusomatic/python/generate_dataset.py | 1 - 1 file changed, 1 deletion(-) diff --git a/neusomatic/python/generate_dataset.py b/neusomatic/python/generate_dataset.py index 400b210..5a90d81 100755 --- a/neusomatic/python/generate_dataset.py +++ b/neusomatic/python/generate_dataset.py @@ -1311,7 +1311,6 @@ def find_records(input_record): records_r = [records[x] for k, w in good_records.items() for x in w] N_none = len(none_records_ids) - thread_logger.info("N_none: {} ".format(N_none)) none_records = list(map(lambda x: records[x], none_records_ids)) none_records = sorted(none_records, key=lambda x: [x[0], int(x[1])]) From fd6e890f6d7342ca1dee0c7d698ef3ef26f8b024 Mon Sep 17 00:00:00 2001 From: Sahraeian Date: Thu, 30 Apr 2020 08:48:19 -0700 Subject: [PATCH 15/15] fix indelrealigner --- neusomatic/python/long_read_indelrealign.py | 55 +++++++++++---------- 1 file changed, 29 insertions(+), 26 deletions(-) diff --git a/neusomatic/python/long_read_indelrealign.py b/neusomatic/python/long_read_indelrealign.py index bc62cca..4a74eda 100755 --- a/neusomatic/python/long_read_indelrealign.py +++ b/neusomatic/python/long_read_indelrealign.py @@ -646,8 +646,8 @@ def find_realign_dict(realign_bed_file, chrom): realign_bed = get_tmp_file() with open(realign_bed_file) as i_f, open(realign_bed, "w") as o_f: for line in skip_empty(i_f): - x=line.strip().split() - if x[0]==chrom: + x = line.strip().split() + if x[0] == chrom: o_f.write(line) realign_dict = {} @@ -923,30 +923,31 @@ def find_var(out_fasta_file, snp_min_af, del_min_af, ins_min_af, scale_maf, simp is_ins = False is_del = False done = False - for i, (r, a) in enumerate(zip(list(ref_seq)+[0], list(alt_seq)+[0])): + for i, (r, a) in enumerate(zip(list(ref_seq) + [0], list(alt_seq) + [0])): if i in i_afs: af = afs[i_afs.index(i)] else: af = 0 if r == a: - done=True + done = True else: - if r==0 and a!=0: + if r == 0 and a != 0: if not is_ins: - done=True - elif r!=0 and a==0: + done = True + elif r != 0 and a == 0: if not is_del: - done=True + done = True else: - done=True + done = True if done: if current_alt: rr = "".join(map(lambda x: NUM_to_NUC[ - x], filter(lambda x: x > 0, current_ref))) + x], filter(lambda x: x > 0, current_ref))) aa = "".join(map(lambda x: NUM_to_NUC[ - x], filter(lambda x: x > 0, current_alt))) - variants.append([current_bias, rr, aa, np.array(current_af)]) - done=False + x], filter(lambda x: x > 0, current_alt))) + variants.append( + [current_bias, rr, aa, np.array(current_af)]) + done = False current_ref = [] current_alt = [] current_af = [] @@ -958,13 +959,14 @@ def find_var(out_fasta_file, snp_min_af, del_min_af, ins_min_af, scale_maf, simp current_ref.append(r) current_alt.append(a) current_af.append(af) - is_ins = r==0 and a!=0 - is_del = r!=0 and a==0 + is_ins = r == 0 and a != 0 + is_del = r != 0 and a == 0 if r != 0: bias += 1 return variants + def TrimREFALT(ref, alt, pos): logger = logging.getLogger(TrimREFALT.__name__) alte = len(alt) @@ -1061,12 +1063,14 @@ def run_realignment(input_record): for var in vars_: pos_, ref_seq, alt_seq, afs = var if ref_seq != alt_seq: - ref, alt, pos = ref_seq, alt_seq, int(region.start) + 1 + pos_ + ref, alt, pos = ref_seq, alt_seq, int( + region.start) + 1 + pos_ if pos > 1: - num_add_before = min(40, pos-1) - before = ref_fasta.fetch(region.chrom, pos - num_add_before, pos-1).upper() + num_add_before = min(40, pos - 1) + before = ref_fasta.fetch( + region.chrom, pos - num_add_before, pos - 1).upper() print(before) - pos -= num_add_before-1 + pos -= num_add_before - 1 ref = before + ref alt = before + alt ref, alt, pos = TrimREFALT( @@ -1096,7 +1100,6 @@ def run_realignment(input_record): if skipped > 0: not_realigned_region = target_region - return all_entries, variants, not_realigned_region shutil.rmtree(bed_tempdir) tempfile.tempdir = original_tempdir return all_entries, variants, not_realigned_region @@ -1228,17 +1231,17 @@ def extend_regions_repeat(region_bed_file, extended_region_bed_file, ref_fasta_f ref_seq = ref_fasta.fetch( chrom, new_start, new_end + 1).upper() if cnt_s == 0: - while check_rep(ref_seq, "left", 3): - new_start -= 3 + while check_rep(ref_seq, "left", 2): + new_start -= 2 ref_seq = ref_fasta.fetch( chrom, new_start, new_end + 1).upper() - cnt_s += 3 + cnt_s += 2 if cnt_s == 0: - while check_rep(ref_seq, "left", 4): - new_start -= 4 + while check_rep(ref_seq, "left", 3): + new_start -= 3 ref_seq = ref_fasta.fetch( chrom, new_start, new_end + 1).upper() - cnt_s += 4 + cnt_s += 3 if cnt_s == 0: while check_rep(ref_seq, "left", 4): new_start -= 4