Question about the preprocessing script bam_cov.py

[whui2bioinformatics]

Hi, It seems that the bam_cov.py script that converts bam file to bigwig file does not involve sequencing depth normalization. I need to compare the difference in predicted results between samples, but the predicted difference is consistent with the difference in sequencing depth!

[davek44]

Correct, we do not perform depth normalization in that script. I generally perform normalization afterward on the model outputs.