From e4fe5c78409e26541ff4f28bfbb19a13ae1ef70c Mon Sep 17 00:00:00 2001
From: Edward S Rice <edward.rice@missouri.edu>
Date: Wed, 21 Sep 2022 06:41:15 -0500
Subject: [PATCH 1/4] Fix a hellish integer size bug

---
 c/kmers.c | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/c/kmers.c b/c/kmers.c
index ddaf3bd..6c8c2d5 100644
--- a/c/kmers.c
+++ b/c/kmers.c
@@ -58,13 +58,13 @@ uint64_t kmer_to_int(char* kmer, unsigned char k) {
                 // no need to waste time doing 'kmer_int |= (0 << (i*2))'
                 break;
             case 'C':
-                kmer_int |= (1 << (i*2));
+                kmer_int |= (1ULL << (i*2));
                 break;
             case 'G':
-                kmer_int |= (2 << (i*2));
+                kmer_int |= (2ULL << (i*2));
                 break;
             case 'T':
-                kmer_int |= (3 << (i*2));
+                kmer_int |= (3ULL << (i*2));
         }
     }
 

From 05fd9f40a7d92dbeb4e703bf8b89e23e24219e5a Mon Sep 17 00:00:00 2001
From: Edward S Rice <edward.rice@missouri.edu>
Date: Wed, 21 Sep 2022 06:41:57 -0500
Subject: [PATCH 2/4] Add python wrappers for a couple other c funcs

---
 src/trio_binning/kmers.py | 21 +++++++++++++++++++++
 1 file changed, 21 insertions(+)

diff --git a/src/trio_binning/kmers.py b/src/trio_binning/kmers.py
index 25d7c32..f7820ef 100644
--- a/src/trio_binning/kmers.py
+++ b/src/trio_binning/kmers.py
@@ -72,6 +72,27 @@ class _HashSet(Structure):
     POINTER(c_int),
 ]
 
+kmer_to_int_c = lib.kmer_to_int
+kmer_to_int_c.argtypes = [c_char_p, c_ubyte]
+kmer_to_int_c.restype = c_uint64
+
+
+def kmer_to_int(kmer: str) -> int:
+    """Convert a kmer to integer format"""
+    return kmer_to_int_c(bytes(kmer, "utf-8"), c_ubyte(len(kmer)))
+
+
+reverse_complement_c = lib.reverse_complement
+reverse_complement_c.argtypes = [c_char_p, c_char_p, c_ubyte]
+
+
+def reverse_complement(kmer: str) -> str:
+    """Reverse complement a k-mer"""
+    out_kmer = bytes("x" * len(kmer), "utf-8")
+    reverse_complement_c(bytes(kmer, "utf-8"), out_kmer, len(kmer))
+    return out_kmer.decode("utf-8")
+
+
 # this is ugly as sin, but necessary because mypy is ok with `pointer`
 # as a subscriptable type while python runtime is not
 if TYPE_CHECKING:

From c3301e3385fa23ca07dd7342adc855116110e856 Mon Sep 17 00:00:00 2001
From: Edward S Rice <edward.rice@missouri.edu>
Date: Wed, 21 Sep 2022 08:01:56 -0500
Subject: [PATCH 3/4] Use uint64 instead of ULL

---
 c/kmers.c | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/c/kmers.c b/c/kmers.c
index 6c8c2d5..341893e 100644
--- a/c/kmers.c
+++ b/c/kmers.c
@@ -58,13 +58,13 @@ uint64_t kmer_to_int(char* kmer, unsigned char k) {
                 // no need to waste time doing 'kmer_int |= (0 << (i*2))'
                 break;
             case 'C':
-                kmer_int |= (1ULL << (i*2));
+                kmer_int |= (UINT64_C(1) << (i*2));
                 break;
             case 'G':
-                kmer_int |= (2ULL << (i*2));
+                kmer_int |= (UINT64_C(2) << (i*2));
                 break;
             case 'T':
-                kmer_int |= (3ULL << (i*2));
+                kmer_int |= (UINT64_C(3) << (i*2));
         }
     }
 
@@ -290,7 +290,7 @@ void count_kmers_in_read(
             kmer[j] = read[i+j];
         if (kmer_in_hash_set(kmer, kmer_revcomp, haplotype_A))
             (*count_A)++;
-        if (kmer_in_hash_set(kmer, kmer_revcomp, haplotype_B))
+        else if (kmer_in_hash_set(kmer, kmer_revcomp, haplotype_B))
             (*count_B)++;
     }
 

From 35a38054c04fdec0641c8676a577fd677b7943fd Mon Sep 17 00:00:00 2001
From: Edward S Rice <edward.rice@missouri.edu>
Date: Wed, 21 Sep 2022 09:40:43 -0500
Subject: [PATCH 4/4] Improve tests

---
 tests/data/hapA.fastq           |   9 ++++---
 tests/data/hapA.txt             |  10 ++++----
 tests/data/hapB.txt             |  10 +++-----
 tests/data/test.ccs.fastq.gz    | Bin 0 -> 44819 bytes
 tests/data/unclassified.fastq   |  12 ----------
 tests/test_classify_by_kmers.py |   3 +--
 tests/test_kmers.py             |  40 ++++++++++++++++++++++++++++----
 7 files changed, 50 insertions(+), 34 deletions(-)
 create mode 100644 tests/data/test.ccs.fastq.gz
 delete mode 100644 tests/data/unclassified.fastq

diff --git a/tests/data/hapA.fastq b/tests/data/hapA.fastq
index 6eb050f..e42adc5 100644
--- a/tests/data/hapA.fastq
+++ b/tests/data/hapA.fastq
@@ -1,4 +1,7 @@
-@readAwesome
-GAGGAGATTTAGAGTGTGAGTCGAGCATAGAGATATATA
+@m64234e_220609_193909/3/ccs
+GTGAGATAGAGAGCACTAATTTCTGACATCCTGTGTGGGTTGTCAGAGCCAGCGCTGCCATCCAAGAGTATCTATCACCTGACGACAGAAGCTTGCCACTGCTATTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCGTGTGTGTGTGTGTGTATGTGCATGCAGGAAGGCATGTGGACACTGTCAGCAACTGTGATCATAATGGGTGCACTGATTTACAGAGTCTAATTCACTTTGTGTTGTTCACTTGCTTGGTGTAGTGAGTGAGCATTTTTAATTTGCCAACTGCTGCATGTGGTTCCTGAGATCTGGAAGAGGACTTTACCTTGTAATGGCTGATTTAACACACTGATGTTTTCATACGCATACAACACATCCTGCTTTGCAAAGTACGATGTAGCAGTGACGTAGCAGTGAGGAAAGAAAGGCTACATGGATTTTATGTTTTACAAAAAAAAAAAAAAGAAAAGAAAGTTCTTGCATTTTGCCTCCTAACCCCTAAGTGAAATCCACTGAAAGCAGTTACCTTCAGACGTCACCTAATGGGTGAGCAGTAAACTCTACTCTTACAAATCATTCATTAAATTATGAATGTGGCTGATTTGTGAAGGCATTAGAGGTTTATTAGTGAACAAACTTCATAGTAGAGACCAAGGAATACGTTGTACACGTCAGGGAGAAATTTGTGAAGGAGGTTAAATTTACTACAGTATCCTAAGCTTTTAACAAAGCTCAGTTCAATCCACAATCTACAGCTGCATTTTATAAAAACAAGGATAGTTACTAAAACTGACAGGCAAGAAGACCATTGCTACAGGATCTCCACATGACATTATACCTGTACACATCAGAGAAGCAGCAAAAAGCTCAATGGTAACTCTGGAGGAGCTGCAGAGATTCACAGCTTTGTTGACAGCATGTCCAACTACTACATTCTATAACTGGTCTTTATAGAAGAGTGGAGTTGAGAAAACCACTTTTGAAAGAATGCTACAGGAATTTTTTGCCATAAGATGACAAAAAAAATATATAGAAATGCTTATTTTTTATTTTATTGTATTTTTTTTTTACGTCACAGAAATGCAGTTTGTGACAAAATGTGAAACTATTCAAGGGATTTGAATACTAGAGTCGCAACACATAAACAAACACATATACATAGGGAAGCTCCTTTCCTGTTGGTATATGTTTATGCTCTTTCTATGAAGTCATAATTTGTAGTCAAAATGTCAATTTATGTGAGATATGTTCTTTTATGTTAATGTATATCTAGACAGATTTTGTTTTGTCTGTTTGTCCATTAGAGGAATCCATAATGAAACGATCCGGAAGTAGAGGAAGGCTTTGAGGAAAATACACATTTAAAAAGCAATACCATCATTAAAATGTTCCTTATGGTGCTGCTCAAATTCAGTGTAAAGCACTGAATTCAAAAGAACTGAAACAAGCATATCTGTCTCTTTTTTTTTTTTCAAATTCATTCTGATAATAAAAATCCTCATTATATAATCAATGCCCATCAAACTTAACCATGAAATATTTCACTGTACATTTTAGGAGGACCTAAATACCGAAGGCAGGATTTCTGCCCTGGAGAACAACTTGAAGATCATGGTGTATGAATATGTCTGCCGGTCACTTTTCAAGGTAAAATACATTCATGTGAGCGAATGGAATTTCTGAGGATCTATGAAAATATCTGTAATCAGTTTTATTTGTTCAGGTGATGAATGGGTAGTGACAAAGTAATGCTTCCTGCTGGCTGCAATTTGTAAACATCGTGTTCTTTGGCAAATTTATTTCAAATCAAGGATAAGCAGTTGATCTAAAATATAAAAACAGTCCACAAATAATATTTTCATATATCCACAAAATCTACTCCCTTTGTCTCTATTAGATACAGAGCTATCTTTTCTTCTTGAGACTAGATTTATGCACTGTTTTATATTCTAAACAGAAATGTGGACTGATACAAAGTGCTTTGATTAGAAATATGACGTCTTACTCTTACCTTGACAAACACCCTTATATTTTTTTAGCAGTTTAATTAGATGTGCATTAATCTCTAACAGTGTTCTTACGGATCCTGTAGGTCAAAGACGCCATCGCATAACCTTAGATATTAACTCCCTTCTGCAGTGTAACCTTTGAAGCCAACACACTAACAATGTGTCACAAGGGTAACAACAGGGTTATTGATTAGGCAGTGGATGTTAATTCTAATGTTTGTGCTCTGAGTGTTGCTATGTTGATCTATCACTTGTGGGATTGTGGGACTTTCAAGCACTGCAGGTGGAGGATAATATATGTATATTTAGTTAACATCACACATCTATGATCAGCAGCAGCTCAGCTACTACCTGATAGAAAACAACTGAATCTTTTCCTCTTCACTCAGGATCAGCATGTGTGCAGCTCACATGATGAAGTGTGGAAAAGATATATAGCAGGTTCACATTAATAGATATAGGTAGGGTGGATAGCTTGCACTGTGGGAAACTAATGATAAAGCTTGAAAAACTAACCCAAACATCTATATGATCAATAGACTAGAAATTGTTACTTCGTACTGTTGAATTAAAATTTTTTTGTATAAAAGCAGATTAATTCAATTAAATTACTAATTTTAACATGGCAGTTTGGAAATATTTCTGGTGCAAAAATACTGTGCACCACCCCTTGTTCTTATTAAGATAAAACTGTTTTAAAACTAAAGTTGTACTTATGTTGTATATAGTAACCTCCTAAAAACTGCTACTAAATTTAGCAGGCAGTATATTCTATTTATAAAAATCAAAATAACTACAAATAAAGGTAACATTTTTTACAAACACAATAGTCAACATATTTACCTGTAGTAGGAATGATCTTTGCTGTTCTGTTTTGAATAACAACTTTTGGGATATCCTATATCTTTTTCATTAAATATTTGGAAAATTTTGTTTTAAAACCATGATACTGTTATAATTTTACTCTGGTAAAACCATCACAATCTCACACTGAGTCATGCCTATTGTTGATTATTGTTGATGGTACACTTCTCACTGAAGAGAACATTACCTGATGGCGCAACAGGAACTGATTAAGCCAATCTAATGAGAATGTTACCTGGAAATGCTGTAAACGGTTTCCACATCTCTGCTCAGTAAAACATGACACATCGTGAAACCGATGTGTGTCCCAAATGCTTGAAATAAAGTGCTAAGCGGAGAAACATTTAGGCTGCGTTCACACTGCAGCCTAAAGTCATCCAATTCCGATATTTTTCGTCAACTCTGATTTTTTTTTTTTTTTGCCGTGGTCGTTCACATTTCCATATAAATGCGACCTGCTTGTGTTCAGCAGTGTGAACGGGAAAACGACCTGAAAGTGTCCCGCATGCGCAGTTGAGGGAGCAATAACAGCGTTCTTGGTGTTCTGCCAACCGCCATAAAAAGAAGAAGTGGTGCTCAGTGTTTGCAGAAGTAAACATGGAGGCTAACGGAGTAGCTTATTTGAAGTTGTTGTCCGACCGGAGCAGCATATTAACAACTTCATCCTCATTATAGTCCGTCATGGTTGTTGTTGTTCTTCCCGCTTGCGCATATCAGGACACAGAATAGTGAGATTTGTCGAGTATCAGTGACATTCAGGTCAGATGGAAGCGACCTGGACGTTAGGTCGCATTTGAATCAGATTCGTATCGGATATGGTCGCATTCGAAAAGAATTTGTGTTGTTCAGACTGTTATGAAAAGTTCAGATAAAGGTCGCACTACGTCAAAAAAAAAAATTGGGTTGAGTCACTTCAGACTGCAGTGTGAATGCAGACCTACTAATTTCAAAAAGGGTATGTTATTTGTCCAAAGATTACCAAGAAATTTCTGATTTCTGCTTCTTGCCAAATCAGAGCTGAATTTATGAATCATAACATGCAACAAAGTTCTTGCGATATAAGGAAAGCCTAAGTCTATAGGTTGTTTCACAGCTAAACATAGAGCTGTAACTGTTGATATTAGAAAGCCACATCTGCACCTGTTGAACTATTTATTGTGTGGAGGTCCGTTACTTCATCCTTCTGTATTTGATTAAATCTACAGACTGGATACAAAGTTACACAGACCGGAATTGCAGAAGTAGAATTCCTATGTAACGTTAGCACTCAAACATTTAAAGCAAAAGTTTCCCAATTTGTCTCCAAAGGCTATTTGTTTTTCTCAGATTTACTAGGCTTACACTAACCATTAGCAAACTCTTCAATGGCAGCATTTATGTATGCAAATGGATATTAAGCTCCTATATGTACTTGGTTTACACATAAGGGAGATCTGACTGAAGCTAAGTGATTAGATCAACAAGGTCTCTTAACTCCGCCACTTTACAAAGCACAGCATCGGCACATATTACTCCTGTATTGTAATTCTAATTCAGCTGTCCTTGCGAGCCACTTGTTAAGTGCATAAAAATTAGATTTACATAAATTTGCATGTCAACCACTGAGGCATAATGCCTGTATTTCATTGTGGGTGTAAATAGGGGCACAGTTTCCATGTTTGTTGTGTTGTTACACAGGAAAGAAAATTGATACTGTCACTCCAGTGACCAGCGTTCATTTATAAAGTCCTATCTTCACTCTCTTTGCAACATGAACTCAAAATATAATTCTCAGATATTTAGGAAAACACATACTGGATTAACAGGGTTTGTGTTAACAATTAAAATCCCTTGGCAACTGTCTCTCCGTTTCTCTCTGTGGGAGCAGGATAAGCATCTAGATTAATGGCTGTATCTGTTATCTTTATTCTAAATCACTATCAGATGTGAAAGACAGTGAGGTAGGTACACCAGAGGACTGTTAGTGTTTAAAGATGATTCAGCAGCCCATGCAGTACTTTCAGTCTTCGGAGAGCTTTTTCCTTTGAGCTTTTACAAATTCGCCAGATTGTGCTCTGCACCAACAACATCCTCTTTTTCAGTCAACATACCGTTTGATCTGTTTTCTATCCATTATTAACTATCCATTATTTTTGATATACTGAAGACAGTTAAACTGAATGTATATAATTGACTTTATGTCTCATCTCCCTCTTGTTGTCTCAGGCTGACCAACTGATGTTTGCAATGCATTTTGTGAAAGGGATGTACTCTGAACTCTTCCAGGAGAATGTGAGTAATTTTTTTTTATTTTTAGGTTGTCTGAAGCCAGTGACAGTTTGATTGCATAAATTCTAGTTTTGCCCAGATGCAACTTTTGACGGAGCTGTTTGTTGCAGGAATGGGAAGTTTTAATCGGATCCATTGTTGGAGAAATGTTTAAAAAAGAGGTAGGGATGCTTATTTATTCATTTGCACCATAAAACAAATATATCTGTCTCTAACGGGGTTTTTTTCGTCAGGAATTCCCTTCTTGGATTGATCAGGAGAGGCATGGAGCAGTGGCCATTTTGAAGGTATGTGCACTAAAATATTATTTAACAGGTTACTTTATTTAAAAGGTGATCAAACATTGAAACAGAACACTAGATATTTAATTCAACTCGAGACATCGGTAGTGTAATATGGGATCAATTAACATTTGATGGCAAGTTTATCAGTAAACAAATCTTTCCCCATCTGAAGATTATATTTGCATCTCTAATGTAATATCTTTCATCCTTGTCAATTTTAACCACCTCTTGCTTTGCAGGCTACTTTCCCAGCTCTGTACCAGACTCTGTGTTTGAGTGACTCAGACCTTTGGGTGTCCTTCTTGCAAAGCTCACAGTGTGAACAGGAAATCCCATCTTCCATCACCAGAAAGATTAGTCTCTTCCAGCAGGTCAGCTTCCATTCAGCTTTTAGCTGTGAAGTAATGAAACTAAATCAGATTTTCCCAGCTGTTTGATTAACGCTGGCTTTCTGTTTTGGCCAGCGAAATCCTTCTAATTTCTGTCACGACAGCAAGCTGTCAATCTCTTTTTATTTCTGATAAACTAATAATTTTAACAAATATTTGTTAAGTAAATAAATCATAGAAAAAAATAAGGGAGTGTTAAATTTTCAAAATGATTTAAAAAATATTAACTATTTCTTCTGACTAGAATATCATCAAATATGTCAATTTATTTCTGTAATTCAATTAAAAAACTCAGATTCATGAGTTATCCACTTGCAGGTGTTTTTTACTTAAGTTTGGTTCATTGTAGCTTAGTGCTAATAAAATTAAAAAATCTGTTTCTCAGACATGTCATGTGTAAGAAACATTTTGTGTCATTTATGTTATAAAGGACCCTTTTTAACAATATGTTATGCTACTGATCGCTTAAAGGTTTTGAGAGGCACGTTTTGAGGTTTTATTTGATCTGAGTTATATTATCCTAAAGTCTGGAAATTGATCACTGTGTGTGGAGTAAAAAATAAAGAAGTGTCTCTTCTTTAACTGAGTTGCTGAAAAAAAAGTATCTTTATATTATATTTGGATATACTGAGATGCACCTGCATATGTTAAAATCCTAGAAATGGCATAAAGTACATTCTTCTTACAATGACTGTACCAGTTGTAAGTTGAAGTCCAACTGTTTAAATGTGTGACTTAACTCCTTCAACATAGGTGAATTCAACCTTTTTTGCAAAGTGTTATTTTCTGATTTTATTTTATTTTATTTTTATTTTGTTACTTGTATGTACTCTACCATATATGCAGCTGCTGTTAATTCAGGCAGTGAGACCTGACCGTCTTCAGAGCGCCATGACAGCCTTTGCCTCTCAAGCTCTTGGTAAGAGTTTTACATTTTTTGTTTAACTTTTAAAGGTTTTGTGGGGGTCCACACTTTATCAGACAAATGCATTTGCACTTCTTAATTATTTCACCTTAATGTTTATTGCGGATGTTGGATGGCATATAATTTGACCAAATAACAGAGCGAGAAAAGAATAACCTTTTTTGAAAGAAATTTAGTTAAATGTTTTCTGTGTTTTCAAAATTCACACATAAATAAAATTGGCTAAAATTGGAGCCATTTGTAGTGGCTTGGCCATCTGGTAAGGATGCCTACTGGATGGCTCCCTGGTGAAGTTTTCTGGCCATTTTGCAATGTTAGGATACCCAGAGGAAGTGGAGAACTATTTTTTCTCGACTGACCTTGGTATTCTCCTGGATGAACTGGCCCAGGGCTTCTCTGATTAGGTTGTTGCCTCTGCAACCCAACCAAGGATTGGGTGAAAGATAATGGATGGATGGGTTTTATCTGTCGGTGCCTATCTCAGTTATTTATCATGACCATCCTCTGGTGGAAGACAAACCTGAGGATGTAGGTTCCTTTTTAACTAAAAGTGTAGCAGCAGGGCTTTACAACATGCGTCATCAGCGTCCTTGAAATATCCACCATTTCCACCCTAATGGGGCAGGGGAGGTAGTGGGAGAGGGTAATTATGTAGGCAAGATTTGAATAGGTGGCTTTGAGAAAAAAATAAAGCTGCCACACAAATCAGCAAAGAGTAGTAATTCACCCAAAATGATGGCAGTATTAAGATATAATAGTTGCTAATAAAAGATATATAAGCTTGCATAATTTGGTAACGAGATAAATGGAGATGACAGTCTGCATTAAGCAAGTTACTGATCAATGTTCTTACCTCCATTATATACAAGACACCCTTTACATGAACCTTACAATTTGTCTCTTTTTTTTTTTTTTGTAGTATTTTATAGCTATACAGCAGTTAAAAATAAAATTAAAGAATACTTACATTTGCTAAGTCTCAGAGAAACGTTTTTCCTACTGCTGCTTTGTGTCATTGTGGTTCTGCATAGTTTGAAGAACATATTCTTCCCGGCACTGACATTTTTTTTTTTTTTTGCCTCACTCTGGAAGAGTCTTGCTGTAACTGTGTCTGTGGGGAGAATATTAACTGGAGACATGTGACCCTCACCTACATTGAATGTATTTAGTAATTCCCTTTCTTGCTCTCACATGGATCAAGGCTCACTGACCAGGATTTCATCTTGTTTCCACATGACTAAAACTAGACCTTATTAGATTTCTACTCCTTCTAACATTTTTGCCAATGAAAAAAATTGCACTAACATATTCATTTTTTTTTTCTCAGAGGTTACACTGTTCTACGTCTGTTATTACTCACTTTCACCTGGTTTTTGTACAATAAAATTGCCATTGCATGAATATTTGCATCATAATTTTAGTCATAGGAAATAAACACTTATATTCTTATAATCTCTGAGGAGTAAAATATATCCCTGTCCGGATGATTCGCAGAATATTCCAAAAGTGATGATGACAAAGTCCTTTTAAGAGTCTGACTAAAATCTTGTCTTCCAAAATTTTAAAATAATTTAGCAATAATTTCATCTTGCTAACATGTAGAGGTTAGAACTTCCAATTTTCAGATTTGAAGAATTTATAAAAATGTTCCCTGATGTTGGAAATCCAGCCGTTAGTAGCCGAAATCTGTTTTGGTATGTAATGAGCACACCCACTGTTTACTGGCACATTTGATTATTTTGATGCTATTAAATTTGTCATCTTATAATTTTTGTAATGCAGAGAAGTACACGACTACTTCTTTATGCTAACAGGGTAACTAAGCATATCAGTTAGCAAGTCTTACTTTTTTGTTTGCTTTTTACTTTTTTTATGTGAAACAGTATACTGGGTTTGACATTATTTCCAGATGCAGATTCTGACATTTGAGGTAATTAGTGCACAACCACCACTAGTTTCTGATCCAGCAAACATTAATGTGCTTGAACAGCTAATATTTTTTACAGTTGGTGCTTATTAGTTAGTGCTTATGATATATTTCCTAATTAACTATCAAACAATTGCAACACAACTTTTTTTGAAGTACCACTGTAAAACCAGCAGTATTTGTTCCCTAGACTTTATGGATTTACATAACATTGAAGCAACAAAGACGGACATTAATGTGTTGCCAGTGTTTTTAAATTATTCTAAATAAAAATAGACTGTTGCATTTAAAAAGTAAGTTTTTTACCTTGAAATAAAACCTTGATCTGCTTGTGTTTTTGACAAATGTGTTGTTTGTTGAATAATATGTCTTGCGTGAATCTCAAACTCAAGAATAATGAGACGATTCCACTAACACGCCTCAGATGATGTTCGTGCAATGCCCACTTAAATTTATCACATTTATTTGGCTTAAAAGCTTGGACTTTCTGCTGCTCTCTGTGTTCACACAATATGAATAAGAATAACTCTCTTGCTTAAGGTTTTTCCTAATGATTTGCCATCCACATTGCAACACTTTTTGCAATGTGGATGGCACATATTGCATTTATCCATTTTTAAAGGTATATTTTGAATTTTATAAAGGTTTACAAATATGTAAGAGTTTGTGTCTATATATCTATCTATCCATCTATATAGATATGTTACGACTGGCTCAAGGTCATAACAAAACAGGAGACCACGCAGTGGTTAAAGTGCATACAAATGGGCTTTTATTAACAAAAACAACAATTGATTGTGGATATCAGTATCAGTGACGTAATGCAAATGCTTGGATGTGTTGTATGATGCATATGGAAGTGTTGAAGTGCAAAAACAAAGACAAACACAAATGTGCAAAAAGGAGGGGAGCTGAGGCCTGGCAACAAAACACCACAAGCGTCAAGCGGCACAGTGGATGCTGAAGGCCACCCAGAAGATGGAGATACCCAGGTTCAAATGCTCTGCGCTCCCAATCAACCTGATCAGCAGACTTTATAATGACAACTTAAACTGTGCACAAATTCTTTATGCTTTAAGTATTTGGGCCAATTGTACCAAAACAAAACAAGATGACATATGTGACCTTAAAAACAAC
 +
-!61161!!!!!!!!!!!!!!!!!***()(!***!))!(%
+~q~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~u~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~w~d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~w~~~~~g~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~U~~~~~~~~~~~~~~~~~~~~~~~~h~~~~~~~~~~~~~~~~~~t~~~~~~~~~~~~~~~~~~~~~~h~~~~~~~~~~~~~~~~~~~~~~~~~x~~~v~~~~~~~~~~~~~~~a~~~Z~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d~~~~~~~~~~~l~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~u~~c~~~~~~~~~~U~~~~~~x~~~~~<~~~~~~~~~~~~~~t~~~~~~~~~~~~~~~~^~~~~m~~m~~~~p~~~~~~~~~~~~~x~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~q~~~~~~~~~~~~~~~~~~~~~~~~~c~~~~~~~~~~~~~~~~~~~~~~~~l~~~~~~~~~~~y~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~v~~~~~~~~~~~~~~~p~~~~~~t~~~~~~~~~~~~~~~~~~~~~~z~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d~~~~~~~w~~~~~a~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~m~~~~|~~~~~~~{~~~~~~~~~~~~~~c~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~t~~~~~~~~~~~~~~~~~~~~~~~~~~~~~]~~~~~~~7~~~~~~~~~~~~~~~~~~~~~\~~~~~~~~~~~~~~~~~~<~~~~~~~~~~~~~~~~~~~~~~~6~~~~~~V~~~~~~~~~J~~~~~~~~~~~~~~~~~~~~~~~~~~z~~~~~~~y~~~~~~~c~~~~~~~~~~s~~~~~~~~~~~~~~~~~~~~~~~~~~~~v~~~~~~~~~~~~~~~~~~u~~~~~~~j~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~j~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~z~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~m~~~~~~~~~~~~~~~~w~~~~~~~~}~~~~~~~~~~~}~{~~~~~~~~~~~~c~~~~~~~~~~~~~~~~~~i~~~~~~~~~~~~~~~~~~~~~~~v~~~~~~~~~~~~~~~~~~~o~j~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~7~~~~~~~~~~~~~~~~~~~~~~~~~~~~~w~~~~~~~~~~~~~~~~~~~~~~~~f~~~~~~~~~~~~~~~~~~~~~~~|~~s~~~~~~~~o~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~e~~~Q~~~~~~~~~~~~~~~~~~~~~~~~~~~~s~~~~~~~~~~~{~O~~~~~~~~~~o~~g~~~~~~~~~~~~~~~~~~~~~~p~g~?~~~~~~x~~~~~~~~|~~~~~~~~~~~~~~~~]~~~~~~~~~~~~w~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~0~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~v~~w~~~~~~~~~~~~~~~~~d~~~~~~~~~~~~~~~~i~~~~~~O~~~~~~~~~~~~j~~~~~~~a~~~~~~~~~~~~~~r~~~~~~~~~t~~l~~~~~~~~~~~~~~~~~~~~~~~~~~z~~~~~~~~~~~~~~~~~~~~~~~~~~~~~i~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~u~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~z~~~~~~~9~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~z~~~~~~~~~~~~~~~~q~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~k~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~e~~~~~~~~~~c~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~q~~~~~z~J~~~~~~~~~~r~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~q~~~s~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~o~~~j~~~~~~~~~~~~~l~~k~~~~~~~~~~~~~~~~~w~~~k~~~~~~~~w~~~~~~~~~~g~~w~~~~~~~~~~~~~~~~~~n~~~~~~~~|~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~w~~~d~~~~~~~~~~i~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~a~~~~n~~~~y~~~~~~~~~n~~~~~~~~~~~~~~~~S~~~~~~~~~~~~~~~~~~~Z~~~~~~q~~~t~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~k~~~~~~~~~~~~\~~g~~~~~~y~~~~~~~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~k~~~~~~~~~~~~~W~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~s~~~~~~~~~~~~~~~~~~~~n~~~~~~~~~~~~~y~~~~~~~~~~v~~~~~~~f~~~~~~~~{~~~~I~~~~~~~~q~~~~w~~~G~~~~~~~~~~~~~~~~~~~~v~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~p~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~s~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~P~~~~~~~~~~~~~~~~6~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~g~~~~~~~k~~~~~~~~~~~~~~~~~~~~~~~~Z~~h~~~~~~~~~~~~~~~~~i~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~p~~~H~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~o~~~~~~~~l~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~v~~~~~~~c~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~y~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~w~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~n~~~~~~~~~~~~~~i~~~~~~~~~~~~~/~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~q~~~h~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~v~~~~~~~~~~~~~~+~~~~~~~~~~~~~a~~~~~~~~~~~z~~~~~~e~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~y~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~y~~s~~~~~~~~~~~~~~o~~~~~~~~~~~~~~~t~~~~~~~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~y~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~h~~r~~~~~~~~~~~~~~~~~~~~~~~~~t~~~~~~~~~~t~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~h~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~se~~~~~~~~~~~~~y~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~l~~~~~~~~~~~~~~~~~~~o~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~k~~~~~~~~~~]~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~~P~~~~~~~~~~~~~~~~~~~~~~~~k~~~~~~~~~~~~~~~S~~~~~~~~~~~~~~~~~~n~~~~~/~~~~~~~~~~~~~~~~m~~~~~~~~~~~~~~~~~~~~~r~~~~l~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~l~~~{~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~x~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~q~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~A~~~~~~~~~~j~~~~u~~~~~~~~t~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~U~~~~~~~~~~~~~~zm~~~~~~~~~~~W~~~~~~~o~~~~~~~~~~~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+ACATTTGTGAATGTCACTGTGTGAACACTCTGACACGGTTGAGCTCTGTGTTTTCTGTCTCACCTGTCTGTGGTTAGAAACTAAATGGAGTGGAGTCATTACTTCAAAGCAAACTTTATTTAGCACTGAAGTTTTGAATAGATCATCACATAATTTCTCTGTGTCCCCACAGCAGGAGTGGATAAATATGACAGGCTTCCTCTGTGCACTGGGGGGTGTATGTCTTCAGCAGCGCAACACTCCAGGACTCGCACTTATAGCCCACCTATGGGCCCACTGAGTGACCGCAAACCCTCCATGATCTCAGTGGGGCCCGGTGACATCACCAACCCGGCTCCCTCTTCCTGCCCTTCATCTACCTCCATCGAGACGCCCGTCAGCCGCTTCCTGGACCGACTGCTGGCTCTGTTGGTGTGTGGCCATGACAGGGTGGGCCTTCATGTCCGCACCAATGTCAAAGACCTACTGGACTGGAACTAAGTCCTGCACTCTATCCCATGCTCTTCAATAAACTGAAAAACAGCATCGGAAAGTTCTCTGACGCTCAGGGACAGGTATGTCTAGCCATTATCTCTTATTAGGAACTCTACAAAAAAACCATGAAAAAGGTAGTGAAACATCTGCAAGCACAATAAAACTGGGAAGAAGGCAGTCTCTGTTTGTGTAGT
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diff --git a/tests/data/hapA.txt b/tests/data/hapA.txt
index a18d8cc..2dd6332 100644
--- a/tests/data/hapA.txt
+++ b/tests/data/hapA.txt
@@ -1,6 +1,4 @@
-CTACCGCCCTCCC
-GAAGATCCTCATC
-GAGGAGATTTAGA
-CTACGGCGCTCAC
-GTGAGCCCCCCGA
-CCTGGGCACACAC
+ACCTCTAAGAAGCTTTGAAAA
+CTTATCATGTCTTTGTTTTCA
+CCAGCTGTGTGATTGGTCCGC
+AAAAATAAGACAGCATGTGAC
diff --git a/tests/data/hapB.txt b/tests/data/hapB.txt
index 9cbc452..0c44f56 100644
--- a/tests/data/hapB.txt
+++ b/tests/data/hapB.txt
@@ -1,7 +1,3 @@
-ATGCGTAGCAGTA
-CATCGCCATCAGC
-CCCCTCCCTGCCC
-GCTCGACTCACAC
-CACTGCGCATCTC
-ATAGAGATATATA
-CTCCCGCCCTCCC
+AACACCAAAAAAAAAAACCTC
+AACCCTGTTGTTACCCTTGTG
+AAACTGTTTTAAAACTAAAGT
diff --git a/tests/data/test.ccs.fastq.gz b/tests/data/test.ccs.fastq.gz
new file mode 100644
index 0000000000000000000000000000000000000000..b23a7c42b32f665b09ccfbc234fd9e792acc568f
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diff --git a/tests/data/unclassified.fastq b/tests/data/unclassified.fastq
deleted file mode 100644
index 5fb7797..0000000
--- a/tests/data/unclassified.fastq
+++ /dev/null
@@ -1,12 +0,0 @@
-@read1
-GATTTGGGGTTCAAAGCAGTATCGATCAAATAGTAAATCCATTTGTTCAACTCACAGTTA
-+
-!''*((((***+))%%%++)(%%%%).1***-+*''))**55CCF>>>>>>CCCCCCC65
-@read2
-GATTTGGGGTTCAAAGCAGTATCGATCAAATAGTAAATCCATTTGTTCAACTCACAGTTA
-+
-!''*((((***+))%%%++)(%%%%).1***-+*''))**55CCF>>>>>>CCCCCCC65
-@read3
-GATTTCGTAGTGTGGGCGCGCGGCGCATCTACGGACGATGTCGCGCGCCGCGATGCTATTA
-+
-!''*((((***+))%%%++)(%%%%).1***-+*''))**55CCF>>>>>>CCCCCCC655
diff --git a/tests/test_classify_by_kmers.py b/tests/test_classify_by_kmers.py
index cc1e9f3..9dca610 100644
--- a/tests/test_classify_by_kmers.py
+++ b/tests/test_classify_by_kmers.py
@@ -17,12 +17,11 @@ def test_classify_by_kmers_help(capsys):
 
 
 def test_classify_by_kmers(capsys, tmpdir):
-    # TODO make better test files
     with patch(
         "sys.argv",
         [
             "classify-by-kmers",
-            join(dirname(__file__), "data", "test.fastq"),
+            join(dirname(__file__), "data", "test.ccs.fastq.gz"),
             join(dirname(__file__), "data", "hapA.txt"),
             join(dirname(__file__), "data", "hapB.txt"),
             "--haplotype-a-out-prefix",
diff --git a/tests/test_kmers.py b/tests/test_kmers.py
index 4217db0..f996b1e 100644
--- a/tests/test_kmers.py
+++ b/tests/test_kmers.py
@@ -1,12 +1,14 @@
 import os.path
 
-import kmers
+import pytest
+
+from trio_binning import kmers
 
 
 def test_build_kmer_hash():
     kmers_path = os.path.join(os.path.dirname(__file__), "data", "hapA.txt")
     kmer_set = kmers.create_kmer_hash_set(kmers_path)
-    assert kmers.get_number_kmers_in_set(kmer_set) == 6
+    assert kmers.get_number_kmers_in_set(kmer_set) == 4
 
 
 def test_count_kmers_in_read():
@@ -17,5 +19,35 @@ def test_count_kmers_in_read():
     hap_b_set = kmers.create_kmer_hash_set(hap_b_path)
 
     assert kmers.count_kmers_in_read(
-        "GAGGAGATTTAGAGTGTGAGTCGAGCATAGAGATATATA", hap_a_set, hap_b_set
-    ) == (1, 2)
+        "CTTATCATGTCTTTGTTTTCAAAGCTTCTTAGAGGTTTTTTTTTTTGGTGTTAATTGGCATAAATTATGGCT",
+        hap_a_set,
+        hap_b_set,
+    ) == (2, 1)
+
+
+@pytest.mark.parametrize(
+    "kmer_str,kmer_int",
+    [
+        ("ATGCTAGCTAGAGAGAGAGGA", 696357446508),
+        ("TTTTTTTTTTTTTTTTTTTTTTTTTTTT", 72057594037927935),
+        ("TTTTTTTTTTTTTTTAGGCCCACTTTTT", 72006304612220927),
+        ("AAAAAAAAAAAAAAAAAAAAAAAAAA", 0),
+        ("GGGAGGGAGGGAGGGAGGGAGGGAGGG", 11868309606246954),
+    ],
+)
+def test_kmer_to_int(kmer_str, kmer_int):
+    assert kmers.kmer_to_int(kmer_str) == kmer_int
+
+
+@pytest.mark.parametrize(
+    "kmer,revcomp_kmer",
+    [
+        ("ATGCTAGCTAGAGAGAGAGGA", "TCCTCTCTCTCTAGCTAGCAT"),
+        ("TTTTTTTTTTTTTTTTTTTTTTTTTTTT", "AAAAAAAAAAAAAAAAAAAAAAAAAAAA"),
+        ("TTTTTTTTTTTTTTTAGGCCCACTTTTT", "AAAAAGTGGGCCTAAAAAAAAAAAAAAA"),
+        ("AAAAAAAAAAAAAAAAAAAAAAAAAA", "TTTTTTTTTTTTTTTTTTTTTTTTTT"),
+        ("GGGAGGGAGGGAGGGAGGGAGGGAGGG", "CCCTCCCTCCCTCCCTCCCTCCCTCCC"),
+    ],
+)
+def test_reverse_complement(kmer, revcomp_kmer):
+    assert kmers.reverse_complement(kmer) == revcomp_kmer