diff --git a/lib/galaxy/workflow/modules.py b/lib/galaxy/workflow/modules.py
index 22d469fd8c80..bf7fffae9d90 100644
--- a/lib/galaxy/workflow/modules.py
+++ b/lib/galaxy/workflow/modules.py
@@ -125,7 +125,7 @@
 # ones.
 RUNTIME_POST_JOB_ACTIONS_KEY = "__POST_JOB_ACTIONS__"
 
-INPUT_PARAMETER_TYPES = Literal["text", "integer", "float", "boolean", "color"]
+INPUT_PARAMETER_TYPES = Literal["text", "integer", "float", "boolean", "color", "directory_uri"]
 POSSIBLE_PARAMETER_TYPES: Tuple[INPUT_PARAMETER_TYPES] = get_args(INPUT_PARAMETER_TYPES)
 
 
@@ -1210,6 +1210,7 @@ def get_inputs(self):
             {"value": "float", "label": "Float"},
             {"value": "boolean", "label": "Boolean (True or False)"},
             {"value": "color", "label": "Color"},
+            {"value": "directory_uri", "label": "Directory URI"},
         ]
         input_parameter_type = SelectToolParameter(None, select_source)
         # encode following loop in description above instead
@@ -1269,17 +1270,7 @@ def get_inputs(self):
             optional_cases = [when_true, when_false]
             optional_cond.cases = optional_cases
 
-            if param_type == "text":
-
-                specify_multiple_source = dict(
-                    name="multiple",
-                    label="Allow multiple selection",
-                    help="Only applies when connected to multi-select parameter(s)",
-                    type="boolean",
-                )
-
-                specify_multiple = BooleanToolParameter(None, specify_multiple_source)
-
+            def regex_validator_definition():
                 add_validators_repeat = Repeat("validators")
                 add_validators_repeat.title = "Add validator to restrict valid input"
                 add_validators_repeat.min = 0
@@ -1306,10 +1297,23 @@ def get_inputs(self):
                     ),
                 }
 
+                return add_validators_repeat
+
+            if param_type == "text":
+
+                specify_multiple_source = dict(
+                    name="multiple",
+                    label="Allow multiple selection",
+                    help="Only applies when connected to multi-select parameter(s)",
+                    type="boolean",
+                )
+
+                specify_multiple = BooleanToolParameter(None, specify_multiple_source)
+
                 # Insert multiple option as first option, which is determined by dictionary insert order
                 when_this_type.inputs = {
                     "multiple": specify_multiple,
-                    "validators": add_validators_repeat,
+                    "validators": regex_validator_definition(),
                     **when_this_type.inputs,
                 }
 
@@ -1427,6 +1431,8 @@ def get_inputs(self):
                         "label": "Set a maximum value for this input",
                     },
                 )
+            if param_type == "directory_uri":
+                when_this_type.inputs["validators"] = regex_validator_definition()
 
             cases.append(when_this_type)
 
@@ -1499,7 +1505,7 @@ def get_runtime_inputs(self, step, connections: Optional[Iterable[WorkflowStepCo
         parameter_type = parameter_def["parameter_type"]
         optional = parameter_def["optional"]
         default_value = parameter_def.get("default", self.default_default_value)
-        if parameter_type not in ["text", "boolean", "integer", "float", "color"]:
+        if parameter_type not in ["text", "boolean", "integer", "float", "color", "directory_uri"]:
             raise ValueError("Invalid parameter type for workflow parameters encountered.")
 
         # Optional parameters for tool input source definition.
@@ -1610,7 +1616,7 @@ def execute(
         input_param = self.get_runtime_inputs(self)["input"]
         # TODO: raise DelayedWorkflowEvaluation if replacement not ready ? Need test
         try:
-            input_param.validate(input_value)
+            input_param.validate(input_value, trans)
         except ValueError as e:
             raise FailWorkflowEvaluation(
                 why=InvocationFailureWorkflowParameterInvalid(
diff --git a/lib/galaxy/workflow/run_request.py b/lib/galaxy/workflow/run_request.py
index a6ca78e3d28c..baeb5492dd6d 100644
--- a/lib/galaxy/workflow/run_request.py
+++ b/lib/galaxy/workflow/run_request.py
@@ -379,7 +379,7 @@ def build_workflow_run_configs(
                 module_injector.inject(step)
                 input_param = step.module.get_runtime_inputs(step.module)["input"]
                 try:
-                    input_param.validate(input_dict)
+                    input_param.validate(input_dict, trans=trans)
                 except ParameterValueError as e:
                     raise exceptions.RequestParameterInvalidException(
                         f"{step.label or step.order_index + 1}: {e.message_suffix}"
diff --git a/lib/galaxy/workflow/workflow_parameter_input_definitions.py b/lib/galaxy/workflow/workflow_parameter_input_definitions.py
index 2b31e793e40e..8cb3bc110b5b 100644
--- a/lib/galaxy/workflow/workflow_parameter_input_definitions.py
+++ b/lib/galaxy/workflow/workflow_parameter_input_definitions.py
@@ -7,12 +7,13 @@
 from galaxy.tools.parameters.basic import (
     BooleanToolParameter,
     ColorToolParameter,
+    DirectoryUriToolParameter,
     FloatToolParameter,
     IntegerToolParameter,
     TextToolParameter,
 )
 
-param_types = Literal["text", "integer", "float", "color", "boolean"]
+param_types = Literal["text", "integer", "float", "color", "boolean", "directory_uri"]
 default_source_type = Dict[str, Union[int, float, bool, str]]
 tool_param_type = Union[
     TextToolParameter,
@@ -20,6 +21,7 @@
     FloatToolParameter,
     BooleanToolParameter,
     ColorToolParameter,
+    DirectoryUriToolParameter,
 ]
 
 
@@ -36,6 +38,7 @@ def get_default_parameter(param_type: param_types) -> tool_param_type:
             FloatToolParameter,
             BooleanToolParameter,
             ColorToolParameter,
+            DirectoryUriToolParameter,
         ] = TextToolParameter(None, default_source)
     elif param_type == "integer":
         input_default_value = IntegerToolParameter(None, default_source)
@@ -45,4 +48,6 @@ def get_default_parameter(param_type: param_types) -> tool_param_type:
         input_default_value = BooleanToolParameter(None, default_source)
     elif param_type == "color":
         input_default_value = ColorToolParameter(None, default_source)
+    elif param_type == "directory_uri":
+        input_default_value = DirectoryUriToolParameter(None, default_source)
     return input_default_value