diff --git a/tools/seqkit/seqkit_locate.xml b/tools/seqkit/seqkit_locate.xml
index e269806d7b4..bfea5493e96 100644
--- a/tools/seqkit/seqkit_locate.xml
+++ b/tools/seqkit/seqkit_locate.xml
@@ -20,7 +20,6 @@ seqkit locate
     --pattern-file '$conditional_pattern.pattern_file'
 #end if
 $output_mode
---validate-seq-length $advanced_options.validate_seq_length
 $advanced_options.circular
 $advanced_options.degenerate
 $advanced_options.hide_matched
@@ -86,7 +85,6 @@ $advanced_options.id_ncbi
             <option value="--bed">BED6</option>
         </param>
         <section name="advanced_options" title="Advanced options">
-            <param argument="--validate-seq-length" type="integer" min="0" value="10000" label="Lenth of the sequence to validate" help="Default: 10000" />
             <param argument="--circular" type="boolean" truevalue="--circular" falsevalue="" checked="false" label="Circular genome" 
                 help="When using flag --circular, end position of matched subsequence that crossing genome sequence end would be greater than sequence length" />
             <param argument="--degenerate" type="boolean" truevalue="--degenerate" falsevalue="" checked="false" label="Pattern/motif contains degenerate bases"