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For Data processing In GSM1827405 author wrote that "Overlapping reads were merged using SeqPrep and split in half with a Awk routine. Paired-ends with one read shorter than 30 bp were discarded."
I run below codein my linux: seqprep -f SRR2017741_1.fastq.gz -r SRR2017741_2.fastq.gz -1 SRR2017741_1_seqprep.fastq.gz -2 SRR2017741_2_seqprep.fastq.gz
and I get below result:
Pairs Processed: 37404976
Pairs Merged: 0
Pairs With Adapters: 75653
Pairs Discarded: 28621
CPU Time Used (Minutes): 55.881797
I don't know why Pairs Merged is 0? I appreciate if you share your comment with me for finding my problem and completing my seqprep code for this sample.
The text was updated successfully, but these errors were encountered:
As a newcomer, I think this is kind of a funny UI decision given that the first line in the description is "SeqPrep is a program to merge paired end Illumina reads that are overlapping into a single longer read"
For Data processing In GSM1827405 author wrote that "Overlapping reads were merged using SeqPrep and split in half with a Awk routine. Paired-ends with one read shorter than 30 bp were discarded."
I run below codein my linux:
seqprep -f SRR2017741_1.fastq.gz -r SRR2017741_2.fastq.gz -1 SRR2017741_1_seqprep.fastq.gz -2 SRR2017741_2_seqprep.fastq.gz
and I get below result:
I don't know why Pairs Merged is 0? I appreciate if you share your comment with me for finding my problem and completing my
seqprep
code for this sample.The text was updated successfully, but these errors were encountered: