From a107a785e1402eaafb36a0d485e41b99331c757e Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Mar=20Gonz=C3=A0lez-Porta?= Date: Wed, 26 Feb 2014 10:24:18 +0000 Subject: [PATCH] Removed exclusive references to the RPKM normalisation method from docs + changed the name of the slot 'rpkm' to 'texp' --- tviz-manual.pdf | Bin 94273 -> 92802 bytes tviz/NAMESPACE | 2 +- tviz/R/AllClasses.R | 2 +- tviz/R/AllGenerics.R | 4 +-- tviz/R/functions.R | 14 +++++----- tviz/R/methods-TranscriptExpressionSet.R | 26 +++++++++--------- .../output-test/ENSG00000124193-distrplot.pdf | Bin 5908 -> 5898 bytes .../output-test/ENSG00000124193-starplot.pdf | Bin 19348 -> 19334 bytes .../output-test/ENSG00000153086-distrplot.pdf | Bin 6652 -> 6641 bytes .../output-test/ENSG00000153086-starplot.pdf | Bin 28534 -> 28517 bytes tviz/inst/tests/test-tviz.R | 8 +++--- tviz/man/TranscriptExpressionSet-class.Rd | 2 +- tviz/man/newTranscriptExpressionSet.Rd | 4 +-- tviz/man/plotDistr.Rd | 2 +- tviz/man/plotStars.Rd | 2 +- tviz/man/readExpressionData.Rd | 4 +-- tviz/man/{rpkms.Rd => texp.Rd} | 12 ++++---- 17 files changed, 41 insertions(+), 41 deletions(-) rename tviz/man/{rpkms.Rd => texp.Rd} (55%) diff --git a/tviz-manual.pdf b/tviz-manual.pdf index 5338cc4174589424842fba9a6abfb3581d36ed0e..f4ceb37e4887601222f096a37ab464598b86bffc 100644 GIT binary patch delta 58026 zcmZU3Q*_|n6J%`LwvCBxPmGB*u_yY)&Lo-Gwr$(CZQJ|p`S07l+|#EY`kwo6tGcVJ zcLy?H5i(W)zW9$(xjU_P);UyADp>@&bBNLH8@7O7BBZGOW_jE26 z@o!1T)C|)hHg6`7@r*4amEFRe22S!EMiQI)CVW3~akSp~pCnEI$Dm9@TQo}{NRdo} zF%*nz^4-bgo>TaasQLAw4x>p%&*1h`VAr?N>++d<+zb}*sJN6JNTUeYK%=M#iP5Ma$`e*?Xj#{kDlx~7ZS!ssbJwvA zV{X`tD$n;bS}J{aPVEck;%l9i)X~T4UBBZZST3_MOr!ecNA&fB3lz^01tdih=}f2K@81V$5~OzuF(KYjWC{WEt(5)>Oq_`dlb^3jzVeXE+|Gr&zbp4tm2T6j3gvQa< zBC5c`p=9x4%;KL3@I=}u6xT~CTOH{Q{>1}SyW=ivU4^!90y1HKhpnWpfw)Iz- zl8@ppsY^^u>QHG*dVLrlp~&*8LDUE=LtKEm3Tg@($PwkwxE#iA17JLUzR$CCwDE** zwbY5~MFI~tJZ;@LM%Y7cJ`VYE=Reva_5V$^5C?s)sO*r!X=@*>EgKO-i<%$AV1uyH 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rpkms, + texp, gexp, dominance, biotypes, diff --git a/tviz/R/AllClasses.R b/tviz/R/AllClasses.R index 1bd44c9..2645739 100644 --- a/tviz/R/AllClasses.R +++ b/tviz/R/AllClasses.R @@ -1,7 +1,7 @@ setClass("TranscriptExpressionSet", representation = representation( id="character", - rpkms="matrix", + texp="matrix", conditions="list", gexp="numeric", dominance="numeric", diff --git a/tviz/R/AllGenerics.R b/tviz/R/AllGenerics.R index 9316243..b07aff5 100644 --- a/tviz/R/AllGenerics.R +++ b/tviz/R/AllGenerics.R @@ -8,8 +8,8 @@ setGeneric("id", setGeneric("conditions", function(object) standardGeneric("conditions")) -setGeneric("rpkms", - function(object) standardGeneric("rpkms")) +setGeneric("texp", + function(object) standardGeneric("texp")) setGeneric("gexp", function(object) standardGeneric("gexp")) diff --git a/tviz/R/functions.R b/tviz/R/functions.R index 7d3eb39..d894b58 100644 --- a/tviz/R/functions.R +++ b/tviz/R/functions.R @@ -7,14 +7,14 @@ readExpressionData=function(gId=gId, infile=infile, cond1=cond1, cond2=cond2) { cond1=.format_cond(cond1) cond2=.format_cond(cond2) - # transcript RPKMs + # transcript expression levels command=paste("grep", gId, infile, sep=" ") data=read.table(pipe(command), col.names=header, colClasses=c("NULL", "character", rep("numeric", length(header)-2))) - rpkms=as.matrix(data[,c(cond1, cond2)]) - rownames(rpkms)=data[,1] + texp=as.matrix(data[,c(cond1, cond2)]) + rownames(texp)=data[,1] - return(rpkms) + return(texp) } readBiotypeData=function(gId=gId, infile=infile) { @@ -40,20 +40,20 @@ readSignificantEvents=function(gId=gId, infile=infile) { return(significant_events) } -newTranscriptExpressionSet=function(gId=gId, rpkms=rpkms, biotypes=biotypes, significant_events=significant_events, cond1=cond1, cond2=cond2) { +newTranscriptExpressionSet=function(gId=gId, texp=texp, biotypes=biotypes, significant_events=significant_events, cond1=cond1, cond2=cond2) { # conditions cond1=.format_cond(cond1) cond2=.format_cond(cond2) cond1=cond1-1 - cond2=seq(from=length(cond1)+1, to=dim(rpkms)[2], by=1) + cond2=seq(from=length(cond1)+1, to=dim(texp)[2], by=1) conditions=list(cond1=cond1, cond2=cond2) # create TranscriptExpressionSet object tes=new("TranscriptExpressionSet", id=gId, conditions=conditions, - rpkms=rpkms, + texp=texp, biotypes=biotypes, significant_events=significant_events ) diff --git a/tviz/R/methods-TranscriptExpressionSet.R b/tviz/R/methods-TranscriptExpressionSet.R index 8fd3a4b..9edc334 100644 --- a/tviz/R/methods-TranscriptExpressionSet.R +++ b/tviz/R/methods-TranscriptExpressionSet.R @@ -1,10 +1,10 @@ ## initialize setMethod("initialize", "TranscriptExpressionSet", - function(.Object, id=id, rpkms=rpkms, biotypes=biotypes, conditions=conditions, significant_events=significant_events){ + function(.Object, id=id, texp=texp, biotypes=biotypes, conditions=conditions, significant_events=significant_events){ .Object@id=id - .Object@rpkms=rpkms + .Object@texp=texp .Object@conditions=conditions - .Object@gexp=colSums(.Object@rpkms) + .Object@gexp=colSums(.Object@texp) .Object@dominance=.calculate_dominance(.Object) .Object@.relexp=.calculate_relexp(.Object) .Object@.scaledexp=.calculate_scaledexp(.Object) @@ -20,9 +20,9 @@ setMethod("show", function(object) { cat("Object of class",class(object),"\n") cat(" Id:",id(object),"\n") - cat(" RPKMs: data frame with", dim(object@rpkms)[1], "transcripts and", dim(object@rpkms)[2], "samples\n") - cat(" transcripts:", head(rownames(object@rpkms), n=4), "(...)\n") - cat(" samples:", head(colnames(object@rpkms), n=4), "(...)\n") + cat(" Transcript expression: data frame with", dim(object@texp)[1], "transcripts and", dim(object@texp)[2], "samples\n") + cat(" transcripts:", head(rownames(object@texp), n=4), "(...)\n") + cat(" samples:", head(colnames(object@texp), n=4), "(...)\n") cat(" Conditions:\n") cat(" condition 1:", head(object@conditions$cond1, n=4), "(...)\n") cat(" condition 2:", head(object@conditions$cond2, n=4), "(...)\n") @@ -44,8 +44,8 @@ setMethod("id", "TranscriptExpressionSet", function(object) object@id) setMethod("conditions", "TranscriptExpressionSet", function(object) object@conditions) -setMethod("rpkms", "TranscriptExpressionSet", - function(object) object@rpkms) +setMethod("texp", "TranscriptExpressionSet", + function(object) object@texp) setMethod("gexp", "TranscriptExpressionSet", function(object) object@gexp) @@ -202,7 +202,7 @@ setMethod(".plot_boxplots", .subplot_boxplots( ldata=list(tes@gexp[tes@conditions$cond1], tes@gexp[tes@conditions$cond2]), xlab=tes@id, - ylab="gene expression (RPKMs)", + ylab="gene expression", type="gexp", mar=mar, col=col, @@ -328,7 +328,7 @@ setMethod(".plot_segments", data1=tes@gexp[tes@conditions$cond1], data2=tes@gexp[tes@conditions$cond2], xlab=tes@id, - ylab="gene expression (RPKMs)", + ylab="gene expression", type="gexp", mar=mar, col=col, @@ -446,7 +446,7 @@ setMethod(".get_legend_title_starplot", "TranscriptExpressionSet", function(object) { legend_title=paste( object@id, "\n\n", - "gene expression summary (RPKMs):\n", + "gene expression summary:\n", " condition 1 (left) - ", .distr_summary(object@gexp[object@conditions$cond1]), "\n", " condition 2 (right) - ", .distr_summary(object@gexp[object@conditions$cond2]), "\n\n", "dominance summary:\n", @@ -459,13 +459,13 @@ setMethod(".get_legend_title_starplot", "TranscriptExpressionSet", setMethod(".calculate_dominance", "TranscriptExpressionSet", function(object) { - dominance=apply(object@rpkms, 2, .ratio_second) + dominance=apply(object@texp, 2, .ratio_second) return(dominance) }) setMethod(".calculate_relexp", "TranscriptExpressionSet", function(object) { - relexp=t(t(object@rpkms)/object@gexp) + relexp=t(t(object@texp)/object@gexp) return(relexp) }) diff --git a/tviz/inst/tests/output-test/ENSG00000124193-distrplot.pdf b/tviz/inst/tests/output-test/ENSG00000124193-distrplot.pdf index c70e15ad375e044ffa56e8571c3d890f4d0957f7..26a86ea9e7cba6cb639f2c5b4162a7b793a36912 100644 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cond2=cond2) +texp=readExpressionData(gId=gId, infile=expdata, cond1=cond1, cond2=cond2) biotypes=readBiotypeData(gId=gId, infile=annot) if (filt != "NA") { significant_events=readSignificantEvents(gId=gId, infile=filt) @@ -30,7 +30,7 @@ if (filt != "NA") { ## create TranscriptExpressionSet object tes=newTranscriptExpressionSet( gId=gId, - rpkms=rpkms, + texp=texp, biotypes=biotypes, cond1=cond1, cond2=cond2, @@ -61,7 +61,7 @@ outdir='./output-test' dir.create(outdir, showWarnings = FALSE) ## load data -rpkms=readExpressionData(gId=gId, infile=expdata, cond1=cond1, cond2=cond2) +texp=readExpressionData(gId=gId, infile=expdata, cond1=cond1, cond2=cond2) biotypes=readBiotypeData(gId=gId, infile=annot) if (filt != "NA") { significant_events=readSignificantEvents(gId=gId, infile=filt) @@ -72,7 +72,7 @@ if (filt != "NA") { ## create TranscriptExpressionSet object tes=newTranscriptExpressionSet( gId=gId, - rpkms=rpkms, + texp=texp, biotypes=biotypes, cond1=cond1, cond2=cond2, diff --git a/tviz/man/TranscriptExpressionSet-class.Rd b/tviz/man/TranscriptExpressionSet-class.Rd index 6ebf68f..d4af328 100644 --- a/tviz/man/TranscriptExpressionSet-class.Rd +++ b/tviz/man/TranscriptExpressionSet-class.Rd @@ -11,7 +11,7 @@ \section{Slots}{ \describe{ \item{gId}{Id for the gene to be plotted.} - \item{rpkms}{Matrix of transcript RPKM/FPKM values in each sample, + \item{texp}{Matrix of transcript expression values in each sample, loaded using the 'readExpressionData' function.} \item{biotypes}{Matrix of biotypes for each of the annotated transcripts within the gene, loaded using the 'readBiotypeData' function.} diff --git a/tviz/man/newTranscriptExpressionSet.Rd b/tviz/man/newTranscriptExpressionSet.Rd index 799d807..914204d 100644 --- a/tviz/man/newTranscriptExpressionSet.Rd +++ b/tviz/man/newTranscriptExpressionSet.Rd @@ -9,7 +9,7 @@ embedded expression data.} \usage{ newTranscriptExpressionSet( gId=gId, - rpkms=rpkms, + texp=texp, biotypes=biotypes, significant_events=significant_events, cond1=cond1, @@ -17,7 +17,7 @@ newTranscriptExpressionSet( } \arguments{ \item{gId}{Id for the gene to be plotted.} - \item{rpkms}{Matrix of transcript RPKM/FPKM values in each sample, + \item{texp}{Matrix of transcript expression values in each sample, loaded using the 'readExpressionData' function.} \item{biotypes}{Matrix of biotypes for each of the annotated transcripts within the gene, loaded using the 'readBiotypeData' function.} diff --git a/tviz/man/plotDistr.Rd b/tviz/man/plotDistr.Rd index 747ac48..d5cbd49 100644 --- a/tviz/man/plotDistr.Rd +++ b/tviz/man/plotDistr.Rd @@ -8,7 +8,7 @@ \description{ A distribution plot contains information on the distribution of gene expression levels, major transcript dominance and transcript relative abundances in each condition. This plot contains three panels: -left - Distribution of gene expression levels (RPKMs or FPKMs). +left - Distribution of gene expression levels. middle - Distribution of major transcript dominance. Major transcript dominance is calculated in each condition as the ratio of expression of the second v.s. first most abundant transcripts. Thus, a ratio close to 0 will indicate that the most abundant transcript is dominating the expression for that gene, while a ratio close to 1 indicates that the first and second most abundant transcripts are expressed at the same level. diff --git a/tviz/man/plotStars.Rd b/tviz/man/plotStars.Rd index 4fec442..1f1873a 100644 --- a/tviz/man/plotStars.Rd +++ b/tviz/man/plotStars.Rd @@ -6,7 +6,7 @@ \title{Create a starplot for the specified TranscriptExpressionSet object.} \description{ -A starplot provides information on the transcript RPKM quantifications in a sample specific manner. +A starplot provides information on the transcript quantifications in a sample specific manner. In this plot each sample is represented as a pie chart, where each of the slices corresponds to a transcript. The size of each slice is proportional to the transcript expression level, and the overall size of the plot is proportional to the gene expression level, thus allowing comparisons across samples. } diff --git a/tviz/man/readExpressionData.Rd b/tviz/man/readExpressionData.Rd index ae1cf6a..7a758f9 100644 --- a/tviz/man/readExpressionData.Rd +++ b/tviz/man/readExpressionData.Rd @@ -5,10 +5,10 @@ \alias{readExpressionData} \title{ - Create a matrix of RPKM/FPKM transcript expression levels for a given gene from the specified input file. + Create a matrix of transcript expression levels for a given gene from the specified input file. } \description{ - Loads transcript expression data (RPKM/FPKM values) for a given gene from the specified input file. + Loads transcript expression data for a given gene from the specified input file. Each sample is represented as a column, and each transcript as a row. } \usage{ diff --git a/tviz/man/rpkms.Rd b/tviz/man/texp.Rd similarity index 55% rename from tviz/man/rpkms.Rd rename to tviz/man/texp.Rd index fc77092..8628404 100644 --- a/tviz/man/rpkms.Rd +++ b/tviz/man/texp.Rd @@ -1,19 +1,19 @@ -\name{rpkms} +\name{texp} \encoding{UTF-8} \Rdversion{1.1} \docType{methods} -\alias{rpkms,TranscriptExpressionSet-method} -\alias{rpkms} +\alias{texp,TranscriptExpressionSet-method} +\alias{texp} \title{ - Accessor function for the 'rpkms' slot of a TranscriptExpressionSet object. + Accessor function for the 'texp' slot of a TranscriptExpressionSet object. } \description{ - The rpkms slot displays a matrix with the initial RPKM/FPKM values provided by the user. Each sample is represented as a column, and each transcript as a row. + The texp slot displays a matrix with the initial expression values provided by the user. Each sample is represented as a column, and each transcript as a row. } \usage{ -\S4method{rpkms}{TranscriptExpressionSet}(object) +\S4method{texp}{TranscriptExpressionSet}(object) } \arguments{