diff --git a/phylogenetic/defaults/clade-i/config.yaml b/phylogenetic/defaults/clade-i/config.yaml
index e8ad850..ea40698 100644
--- a/phylogenetic/defaults/clade-i/config.yaml
+++ b/phylogenetic/defaults/clade-i/config.yaml
@@ -19,14 +19,14 @@ auspice_name: "mpox_clade-I"
 filter:
   min_date: 1900
   min_length: 100000
-  exclude_where: 'clade!=I'
 
 
-### We don't want to subsample, so specify a config which is essentially a no-op
+### Filter to only Clade I sequences
 subsample:
   everything:
     group_by: ""
     sequences_per_group: ""
+    query: "'clade in [\"I\", \"Ia\", \"Ib\"]'"
 
 ## align
 max_indel: 10000
diff --git a/phylogenetic/rules/prepare_sequences.smk b/phylogenetic/rules/prepare_sequences.smk
index 4916379..2fc7ce8 100644
--- a/phylogenetic/rules/prepare_sequences.smk
+++ b/phylogenetic/rules/prepare_sequences.smk
@@ -98,6 +98,11 @@ rule subsample:
         sequences_per_group=lambda w: config["subsample"][w.sample][
             "sequences_per_group"
         ],
+        query=lambda w: (
+            f"--query {config['subsample'][w.sample]['query']}"
+            if "query" in config["subsample"][w.sample]
+            else ""
+        ),
         other_filters=lambda w: config["subsample"][w.sample].get("other_filters", ""),
         exclude=lambda w: (
             f"--exclude-where {' '.join([f'lineage={l}' for l in config['subsample'][w.sample]['exclude_lineages']])}"
@@ -113,6 +118,7 @@ rule subsample:
             --output-strains {output.strains} \
             {params.group_by} \
             {params.sequences_per_group} \
+            {params.query} \
             {params.exclude} \
             {params.other_filters} \
             --output-log {output.log}