diff --git a/modules/nf-core/wittyer/Dockerfile b/modules/nf-core/wittyer/Dockerfile
index fae697ab9dc3..da964cd74542 100644
--- a/modules/nf-core/wittyer/Dockerfile
+++ b/modules/nf-core/wittyer/Dockerfile
@@ -1,11 +1,11 @@
-FROM mcr.microsoft.com/dotnet/sdk:6.0@sha256:6df1177e48b55272316d08f19cb383483af82aca5cdc67a76c414bc200847624 as builder
+FROM mcr.microsoft.com/dotnet/sdk:9.0@sha256:7d24e90a392e88eb56093e4eb325ff883ad609382a55d42f17fd557b997022ca as builder
 WORKDIR /src
 COPY . /src
 RUN cd Wittyer \
     && dotnet publish -f net6.0 -r linux-x64 -c Release -o /output \
     && chmod +x /output/Wittyer
 
-FROM mcr.microsoft.com/dotnet/runtime:6.0@sha256:437cda84bdce26ce074d88b63abeec567c7226d73e8b911605077468e1d5c8d5
+FROM mcr.microsoft.com/dotnet/runtime:9.0@sha256:1e5eb0ed94ca96a34a914456db80e48bd1bb7bc3e3c8eda5e2c3d89c153c3081
 LABEL git_repository=https://git.illumina.com/DASTE/Ilmn.Das.App.Wittyer.git
 WORKDIR /opt/Wittyer
 RUN apt-get -y update && apt-get -y install tabix libunwind8 openssl procps