From 4475b7e334a6eca6b65bf999c5ce0f3f65dba919 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 19 Dec 2023 19:47:00 +0000 Subject: [PATCH 01/61] Template update for nf-core/tools version 2.11 --- .github/CONTRIBUTING.md | 3 + .github/PULL_REQUEST_TEMPLATE.md | 1 + .github/workflows/ci.yml | 2 +- .github/workflows/fix-linting.yml | 4 +- .github/workflows/linting.yml | 12 +- .gitpod.yml | 4 +- .nf-core.yml | 2 +- README.md | 17 +-- assets/multiqc_config.yml | 2 +- assets/slackreport.json | 2 +- conf/modules.config | 2 +- docs/usage.md | 4 +- lib/NfcoreTemplate.groovy | 32 ++--- modules.json | 6 +- .../dumpsoftwareversions/environment.yml | 7 ++ .../custom/dumpsoftwareversions/main.nf | 6 +- .../custom/dumpsoftwareversions/meta.yml | 7 +- .../dumpsoftwareversions/tests/main.nf.test | 38 ++++++ .../tests/main.nf.test.snap | 27 +++++ .../dumpsoftwareversions/tests/tags.yml | 2 + modules/nf-core/fastqc/environment.yml | 7 ++ modules/nf-core/fastqc/main.nf | 10 +- modules/nf-core/fastqc/meta.yml | 5 + modules/nf-core/fastqc/tests/main.nf.test | 109 ++++++++++++++++++ .../nf-core/fastqc/tests/main.nf.test.snap | 10 ++ modules/nf-core/fastqc/tests/tags.yml | 2 + modules/nf-core/multiqc/environment.yml | 7 ++ modules/nf-core/multiqc/main.nf | 8 +- modules/nf-core/multiqc/meta.yml | 11 +- modules/nf-core/multiqc/tests/main.nf.test | 63 ++++++++++ modules/nf-core/multiqc/tests/tags.yml | 2 + nextflow.config | 8 +- nextflow_schema.json | 16 +-- 33 files changed, 361 insertions(+), 77 deletions(-) create mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml create mode 100644 modules/nf-core/fastqc/environment.yml create mode 100644 modules/nf-core/fastqc/tests/main.nf.test create mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap create mode 100644 modules/nf-core/fastqc/tests/tags.yml create mode 100644 modules/nf-core/multiqc/environment.yml create mode 100644 modules/nf-core/multiqc/tests/main.nf.test create mode 100644 modules/nf-core/multiqc/tests/tags.yml diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 1189151..cde3aa6 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -27,6 +27,9 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests +You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to +receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. + When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index b716bb3..775ca31 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -19,6 +19,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rang - [ ] If necessary, also make a PR on the nf-core/rangeland _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. - [ ] `CHANGELOG.md` is updated. diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index b65ba9a..226bce4 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,7 +28,7 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index c57b539..6286d83 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -13,7 +13,7 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 with: token: ${{ secrets.nf_core_bot_auth_token }} @@ -24,7 +24,7 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install Prettier run: npm install -g prettier @prettier/plugin-php diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index b8bdd21..905c58e 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,9 +14,9 @@ jobs: EditorConfig: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install editorconfig-checker run: npm install -g editorconfig-checker @@ -27,9 +27,9 @@ jobs: Prettier: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install Prettier run: npm install -g prettier @@ -40,7 +40,7 @@ jobs: PythonBlack: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - name: Check code lints with Black uses: psf/black@stable @@ -71,7 +71,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 diff --git a/.gitpod.yml b/.gitpod.yml index 25488dc..acf7269 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -4,7 +4,9 @@ tasks: command: | pre-commit install --install-hooks nextflow self-update - + - name: unset JAVA_TOOL_OPTIONS + command: | + unset JAVA_TOOL_OPTIONS vscode: extensions: # based on nf-core.nf-core-extensionpack - codezombiech.gitignore # Language support for .gitignore files diff --git a/.nf-core.yml b/.nf-core.yml index 96b0d30..af643f6 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -2,4 +2,4 @@ repository_type: pipeline template: prefix: nf-core skip: - - igenomes + - igenomes diff --git a/README.md b/README.md index eb73b46..37605da 100644 --- a/README.md +++ b/README.md @@ -30,11 +30,8 @@ ## Usage -:::note -If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how -to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) -with `-profile test` before running the workflow on actual data. -::: +> [!NOTE] +> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. - + diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 3bf0482..2d0c6ee 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,5 +1,5 @@ report_comment: > - This report has been generated by the nf-core/rangeland + This report has been generated by the nf-core/rangeland analysis pipeline. For information about how to interpret these results, please see the documentation. report_section_order: diff --git a/assets/slackreport.json b/assets/slackreport.json index af53fb9..50d914f 100644 --- a/assets/slackreport.json +++ b/assets/slackreport.json @@ -3,7 +3,7 @@ { "fallback": "Plain-text summary of the attachment.", "color": "<% if (success) { %>good<% } else { %>danger<%} %>", - "author_name": "nf-core/rangeland v${version} - ${runName}", + "author_name": "nf-core/rangeland ${version} - ${runName}", "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", "fields": [ diff --git a/conf/modules.config b/conf/modules.config index 39e8138..d91c6ab 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -39,7 +39,7 @@ process { } withName: 'MULTIQC' { - ext.args = params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' + ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ path: { "${params.outdir}/multiqc" }, mode: params.publish_dir_mode, diff --git a/docs/usage.md b/docs/usage.md index 286718c..a4ed357 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -20,7 +20,7 @@ You will need to create a samplesheet with information about the samples you wou The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: -```console +```csv title="samplesheet.csv" sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz @@ -33,7 +33,7 @@ The pipeline will auto-detect whether a sample is single- or paired-end using th A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. -```console +```csv title="samplesheet.csv" sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy index 01b8653..e248e4c 100755 --- a/lib/NfcoreTemplate.groovy +++ b/lib/NfcoreTemplate.groovy @@ -4,6 +4,7 @@ import org.yaml.snakeyaml.Yaml import groovy.json.JsonOutput +import nextflow.extension.FilesEx class NfcoreTemplate { @@ -141,12 +142,14 @@ class NfcoreTemplate { try { if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } [ 'sendmail', '-t' ].execute() << sendmail_html log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" } catch (all) { // Catch failures and try with plaintext def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { mail_cmd += [ '-A', mqc_report ] } mail_cmd.execute() << email_html @@ -155,14 +158,16 @@ class NfcoreTemplate { } // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") + FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() } // @@ -227,15 +232,14 @@ class NfcoreTemplate { // Dump pipeline parameters in a json file // public static void dump_parameters(workflow, params) { - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def output_pf = new File(output_d, "params_${timestamp}.json") + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") def jsonStr = JsonOutput.toJson(params) - output_pf.text = JsonOutput.prettyPrint(jsonStr) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() } // diff --git a/modules.json b/modules.json index f2f2a26..93e746a 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "bd8092b67b5103bdd52e300f75889442275c3117", + "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "4ab13872435962dadc239979554d13709e20bf29", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml new file mode 100644 index 0000000..f0c63f6 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -0,0 +1,7 @@ +name: custom_dumpsoftwareversions +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.17 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index ebc8727..7685b33 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : + 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml index c32657d..5f15a5f 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml @@ -1,4 +1,4 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: custom_dumpsoftwareversions description: Custom module used to dump software versions within the nf-core pipeline template keywords: @@ -16,7 +16,6 @@ input: type: file description: YML file containing software versions pattern: "*.yml" - output: - yml: type: file @@ -30,7 +29,9 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@drpatelh" - "@grst" +maintainers: + - "@drpatelh" + - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test new file mode 100644 index 0000000..eec1db1 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -0,0 +1,38 @@ +nextflow_process { + + name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" + script "../main.nf" + process "CUSTOM_DUMPSOFTWAREVERSIONS" + tag "modules" + tag "modules_nfcore" + tag "custom" + tag "dumpsoftwareversions" + tag "custom/dumpsoftwareversions" + + test("Should run without failures") { + when { + process { + """ + def tool1_version = ''' + TOOL1: + tool1: 0.11.9 + '''.stripIndent() + + def tool2_version = ''' + TOOL2: + tool2: 1.9 + '''.stripIndent() + + input[0] = Channel.of(tool1_version, tool2_version).collectFile() + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap new file mode 100644 index 0000000..4274ed5 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -0,0 +1,27 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ], + "1": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "2": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "mqc_yml": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "versions": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "yml": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ] + } + ], + "timestamp": "2023-11-03T14:43:22.157011" + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml new file mode 100644 index 0000000..405aa24 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml @@ -0,0 +1,2 @@ +custom/dumpsoftwareversions: + - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml new file mode 100644 index 0000000..1787b38 --- /dev/null +++ b/modules/nf-core/fastqc/environment.yml @@ -0,0 +1,7 @@ +name: fastqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 249f906..9e19a74 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -2,10 +2,10 @@ process FASTQC { tag "$meta.id" label 'process_medium' - conda "bioconda::fastqc=0.11.9" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' : - 'biocontainers/fastqc:0.11.9--0' }" + 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : + 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" input: tuple val(meta), path(reads) @@ -37,7 +37,7 @@ process FASTQC { cat <<-END_VERSIONS > versions.yml "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) END_VERSIONS """ @@ -49,7 +49,7 @@ process FASTQC { cat <<-END_VERSIONS > versions.yml "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) END_VERSIONS """ } diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml index 4da5bb5..ee5507e 100644 --- a/modules/nf-core/fastqc/meta.yml +++ b/modules/nf-core/fastqc/meta.yml @@ -50,3 +50,8 @@ authors: - "@grst" - "@ewels" - "@FelixKrueger" +maintainers: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test new file mode 100644 index 0000000..b9e8f92 --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -0,0 +1,109 @@ +nextflow_process { + + name "Test Process FASTQC" + script "../main.nf" + process "FASTQC" + tag "modules" + tag "modules_nfcore" + tag "fastqc" + + test("Single-Read") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id: 'test', single_end:true ], + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. + // looks like this:
Mon 2 Oct 2023
test.gz
+ // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 + { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, + { assert path(process.out.html.get(0).get(1)).getText().contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match("versions") }, + { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + ) + } + } +// TODO +// // +// // Test with paired-end data +// // +// workflow test_fastqc_paired_end { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with interleaved data +// // +// workflow test_fastqc_interleaved { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with bam data +// // +// workflow test_fastqc_bam { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with multiple samples +// // +// workflow test_fastqc_multiple { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with custom prefix +// // +// workflow test_fastqc_custom_prefix { +// input = [ +// [ id:'mysample', single_end:true ], // meta map +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } +} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap new file mode 100644 index 0000000..636a32c --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -0,0 +1,10 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2023-10-09T23:40:54+0000" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml new file mode 100644 index 0000000..7834294 --- /dev/null +++ b/modules/nf-core/fastqc/tests/tags.yml @@ -0,0 +1,2 @@ +fastqc: + - modules/nf-core/fastqc/** diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml new file mode 100644 index 0000000..bc0bdb5 --- /dev/null +++ b/modules/nf-core/multiqc/environment.yml @@ -0,0 +1,7 @@ +name: multiqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.18 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1fc387b..00cc48d 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -1,10 +1,10 @@ process MULTIQC { label 'process_single' - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : + 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -25,12 +25,14 @@ process MULTIQC { def args = task.ext.args ?: '' def config = multiqc_config ? "--config $multiqc_config" : '' def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' + def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' """ multiqc \\ --force \\ $args \\ $config \\ $extra_config \\ + $logo \\ . cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f93b5ee..f1aa660 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,5 +1,5 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json -name: MultiQC +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: - QC @@ -13,7 +13,6 @@ tools: homepage: https://multiqc.info/ documentation: https://multiqc.info/docs/ licence: ["GPL-3.0-or-later"] - input: - multiqc_files: type: file @@ -31,7 +30,6 @@ input: type: file description: Optional logo file for MultiQC pattern: "*.{png}" - output: - report: type: file @@ -54,3 +52,8 @@ authors: - "@bunop" - "@drpatelh" - "@jfy133" +maintainers: + - "@abhi18av" + - "@bunop" + - "@drpatelh" + - "@jfy133" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test new file mode 100644 index 0000000..c2dad21 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -0,0 +1,63 @@ +nextflow_process { + + name "Test Process MULTIQC" + script "../main.nf" + process "MULTIQC" + tag "modules" + tag "modules_nfcore" + tag "multiqc" + + test("MULTIQC: FASTQC") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.report.get(0)).exists() }, + { assert path(process.out.data.get(0)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + ) + } + + } + + test("MULTIQC: FASTQC and a config file") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.report.get(0)).exists() }, + { assert path(process.out.data.get(0)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + ) + } + + } +} diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml new file mode 100644 index 0000000..bea6c0d --- /dev/null +++ b/modules/nf-core/multiqc/tests/tags.yml @@ -0,0 +1,2 @@ +multiqc: + - modules/nf-core/multiqc/** diff --git a/nextflow.config b/nextflow.config index 3c2b52b..9410428 100644 --- a/nextflow.config +++ b/nextflow.config @@ -78,6 +78,7 @@ profiles { dumpHashes = true process.beforeScript = 'echo $HOSTNAME' cleanup = false + nextflow.enable.configProcessNamesValidation = true } conda { conda.enabled = true @@ -100,13 +101,13 @@ profiles { } docker { docker.enabled = true - docker.userEmulation = true conda.enabled = false singularity.enabled = false podman.enabled = false shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false + runOptions = '-u $(id -u):$(id -g)' } arm { docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' @@ -177,7 +178,7 @@ singularity.registry = 'quay.io' // Nextflow plugins plugins { - id 'nf-validation' // Validation of pipeline parameters and creation of an input channel from a sample sheet + id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet } // Export these variables to prevent local Python/R libraries from conflicting with those in the container @@ -194,6 +195,9 @@ env { // Capture exit codes from upstream processes when piping process.shell = ['/bin/bash', '-euo', 'pipefail'] +// Disable process selector warnings by default. Use debug profile to enable warnings. +nextflow.enable.configProcessNamesValidation = false + def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { enabled = true diff --git a/nextflow_schema.json b/nextflow_schema.json index 34856c4..b418065 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,10 +10,7 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "input", - "outdir" - ], + "required": ["input", "outdir"], "properties": { "input": { "type": "string", @@ -171,14 +168,7 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], "hidden": true }, "email_on_fail": { @@ -282,4 +272,4 @@ "$ref": "#/definitions/generic_options" } ] -} \ No newline at end of file +} From a9012e80f661c464ad7a10119ff6a722e635da4e Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Wed, 20 Dec 2023 15:57:55 +0000 Subject: [PATCH 02/61] Template update for nf-core/tools version 2.11.1 --- .../{release-announcments.yml => release-announcements.yml} | 0 nextflow.config | 4 ++-- 2 files changed, 2 insertions(+), 2 deletions(-) rename .github/workflows/{release-announcments.yml => release-announcements.yml} (100%) diff --git a/.github/workflows/release-announcments.yml b/.github/workflows/release-announcements.yml similarity index 100% rename from .github/workflows/release-announcments.yml rename to .github/workflows/release-announcements.yml diff --git a/nextflow.config b/nextflow.config index 9410428..5b0d857 100644 --- a/nextflow.config +++ b/nextflow.config @@ -107,10 +107,10 @@ profiles { shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false - runOptions = '-u $(id -u):$(id -g)' + docker.runOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { singularity.enabled = true From 30d36cbae6cc4571ad0d7b4ba1a680a41f3a31c2 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Wed, 24 Jan 2024 13:34:42 +0100 Subject: [PATCH 03/61] Moved to a more stable version of FORCE --- modules/local/force-generate_analysis_mask.nf | 2 +- modules/local/force-generate_tile_allow_list.nf | 2 +- modules/local/force-higher_level.nf | 4 ++-- modules/local/force-mosaic.nf | 2 +- modules/local/force-preprocess.nf | 2 +- modules/local/force-pyramid.nf | 2 +- modules/local/higher_level_force_config.nf | 2 +- modules/local/merge.nf | 2 +- modules/local/preprocess_force_config.nf | 2 +- 9 files changed, 10 insertions(+), 10 deletions(-) diff --git a/modules/local/force-generate_analysis_mask.nf b/modules/local/force-generate_analysis_mask.nf index ba8ce54..b5ee8b4 100644 --- a/modules/local/force-generate_analysis_mask.nf +++ b/modules/local/force-generate_analysis_mask.nf @@ -4,7 +4,7 @@ process FORCE_GENERATE_ANALYSIS_MASK{ label 'process_single' - container "docker.io/davidfrantz/force:3.7.11" + container "docker.io/davidfrantz/force:3.7.10" input: path aoi diff --git a/modules/local/force-generate_tile_allow_list.nf b/modules/local/force-generate_tile_allow_list.nf index 2a7bb70..4ea0e19 100644 --- a/modules/local/force-generate_tile_allow_list.nf +++ b/modules/local/force-generate_tile_allow_list.nf @@ -4,7 +4,7 @@ process FORCE_GENERATE_TILE_ALLOW_LIST{ label 'process_single' - container "docker.io/davidfrantz/force:3.7.11" + container "docker.io/davidfrantz/force:3.7.10" input: path aoi diff --git a/modules/local/force-higher_level.nf b/modules/local/force-higher_level.nf index 8a2a644..dfd8d80 100644 --- a/modules/local/force-higher_level.nf +++ b/modules/local/force-higher_level.nf @@ -4,14 +4,14 @@ process FORCE_HIGHER_LEVEL { label 'process_high' - container "docker.io/davidfrantz/force:3.7.11" + container "docker.io/davidfrantz/force:3.7.10" tag { tile } input: tuple val(tile), path(config), path(ard), path(aoi), path (datacube), path (endmember) output: - path 'trend/*.tif*', emit: trend_files + path 'trend/*.tif*', optional:true, emit: trend_files path "versions.yml", emit: versions when: diff --git a/modules/local/force-mosaic.nf b/modules/local/force-mosaic.nf index 519a5d0..2465b07 100644 --- a/modules/local/force-mosaic.nf +++ b/modules/local/force-mosaic.nf @@ -5,7 +5,7 @@ process FORCE_MOSAIC{ label 'process_low' tag { product } - container "docker.io/davidfrantz/force:3.7.11" + container "docker.io/davidfrantz/force:3.7.10" input: tuple val(product), path('trend/*') diff --git a/modules/local/force-preprocess.nf b/modules/local/force-preprocess.nf index 999d143..c28af6a 100644 --- a/modules/local/force-preprocess.nf +++ b/modules/local/force-preprocess.nf @@ -5,7 +5,7 @@ process FORCE_PREPROCESS { label 'process_medium' tag { data.simpleName } - container "docker.io/davidfrantz/force:3.7.11" + container "docker.io/davidfrantz/force:3.7.10" input: tuple path(conf), path(data), path(cube), path(tile), path(dem), path(wvdb) diff --git a/modules/local/force-pyramid.nf b/modules/local/force-pyramid.nf index 98f6d5e..e6eb9c6 100644 --- a/modules/local/force-pyramid.nf +++ b/modules/local/force-pyramid.nf @@ -5,7 +5,7 @@ process FORCE_PYRAMID { label 'process_low' tag { tile } - container "docker.io/davidfrantz/force:3.7.11" + container "docker.io/davidfrantz/force:3.7.10" input: tuple val(tile), path(image) diff --git a/modules/local/higher_level_force_config.nf b/modules/local/higher_level_force_config.nf index 5e9eac7..3be1036 100644 --- a/modules/local/higher_level_force_config.nf +++ b/modules/local/higher_level_force_config.nf @@ -5,7 +5,7 @@ process HIGHER_LEVEL_CONFIG { label 'process_single' tag { tile } - container "docker.io/davidfrantz/force:3.7.11" + container "docker.io/davidfrantz/force:3.7.10" input: tuple val(tile), path("ard/${tile}/*"), path("ard/${tile}/*"), path("mask/${tile}/aoi.tif") diff --git a/modules/local/merge.nf b/modules/local/merge.nf index fb7a1b7..55b0d52 100644 --- a/modules/local/merge.nf +++ b/modules/local/merge.nf @@ -5,7 +5,7 @@ process MERGE { label 'process_low' tag { id } - container 'docker.io/davidfrantz/force:dev' + container "docker.io/davidfrantz/force:3.7.10" input: val (data_type) // defines whether qai or boa is merged diff --git a/modules/local/preprocess_force_config.nf b/modules/local/preprocess_force_config.nf index 2830ea7..e759ac8 100644 --- a/modules/local/preprocess_force_config.nf +++ b/modules/local/preprocess_force_config.nf @@ -5,7 +5,7 @@ process PREPROCESS_CONFIG { label 'process_single' tag { data.simpleName } - container "docker.io/davidfrantz/force:3.7.11" + container "docker.io/davidfrantz/force:3.7.10" input: path data From 9beb427d4e27f6d52e50ecdb9ad3fddec9def243 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Wed, 24 Jan 2024 13:36:07 +0100 Subject: [PATCH 04/61] Fixed publishDir for visualization processes --- conf/modules.config | 19 +++++++++++++------ 1 file changed, 13 insertions(+), 6 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 95b8962..9f8a3df 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -111,10 +111,17 @@ process { time = { 20.minute * task.attempt } stageInMode = 'copy' publishDir = [ - path: "${params.outdir}/trend/pyramid/", - saveAs: { "${it.substring(12,it.indexOf("."))}/trend/${it.substring(0,11)}/$it" }, - pattern: '*.tif*', - mode: params.publish_dir_mode + [ + path: { "${params.outdir}/trend/pyramid/" }, + saveAs: { "${it.substring(12,it.indexOf("."))}/trend/${it.substring(0,11)}/$it" }, + pattern: '*.tif*', + mode: params.publish_dir_mode + ], + [ + path: { "${params.outdir}/trend/pyramid/" }, + pattern: 'versions.yml', + mode: params.publish_dir_mode + ] ] } @@ -122,9 +129,9 @@ process { memory = { 1500.MB * task.attempt } time = { 20.minute * task.attempt } publishDir = [ - path: "${params.outdir}/trend/mosaic/", + path: { "${params.outdir}/trend/mosaic/" }, mode: params.publish_dir_mode, - saveAs: { it.equals('versions.yml') ? null : "${params.outdir}/trend/mosaic/${task.tag}/${it.replaceAll("trend/","")}"} + saveAs: {"${params.outdir}/trend/mosaic/${task.tag}/${it.replaceAll("trend/","")}"} ] } From d760cc79cf378f698b97e53bf07a3b20e8341310 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 29 Jan 2024 15:13:17 +0000 Subject: [PATCH 05/61] Template update for nf-core/tools version 2.12 --- .editorconfig | 8 + .github/workflows/awsfulltest.yml | 2 +- .github/workflows/awstest.yml | 2 +- .github/workflows/branch.yml | 2 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 67 +++++ .github/workflows/fix-linting.yml | 72 +++-- .github/workflows/linting.yml | 61 +--- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 4 +- .gitpod.yml | 1 + .pre-commit-config.yaml | 7 +- README.md | 8 +- assets/email_template.html | 2 +- assets/email_template.txt | 2 +- assets/nf-core-rangeland_logo_light.png | Bin 74024 -> 77297 bytes docs/images/nf-core-rangeland_logo_dark.png | Bin 74160 -> 29686 bytes docs/images/nf-core-rangeland_logo_light.png | Bin 74024 -> 25410 bytes lib/WorkflowMain.groovy | 16 +- lib/nfcore_external_java_deps.jar | Bin 2291171 -> 0 bytes main.nf | 2 +- modules.json | 6 +- .../dumpsoftwareversions/environment.yml | 2 +- .../custom/dumpsoftwareversions/main.nf | 4 +- .../dumpsoftwareversions/tests/main.nf.test | 7 +- .../tests/main.nf.test.snap | 50 ++-- modules/nf-core/fastqc/tests/main.nf.test | 265 ++++++++++++------ .../nf-core/fastqc/tests/main.nf.test.snap | 12 +- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 6 +- modules/nf-core/multiqc/meta.yml | 1 - modules/nf-core/multiqc/tests/main.nf.test | 48 +++- .../nf-core/multiqc/tests/main.nf.test.snap | 21 ++ nextflow.config | 3 +- pyproject.toml | 17 +- workflows/rangeland.nf | 7 + 36 files changed, 490 insertions(+), 221 deletions(-) create mode 100644 .github/workflows/download_pipeline.yml delete mode 100644 lib/nfcore_external_java_deps.jar create mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap diff --git a/.editorconfig b/.editorconfig index b6b3190..9b99008 100644 --- a/.editorconfig +++ b/.editorconfig @@ -22,3 +22,11 @@ indent_size = unset [/assets/email*] indent_size = unset + +# ignore Readme +[README.md] +indent_style = unset + +# ignore python +[*.{py}] +indent_style = unset diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index e55fba7..567c485 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@v3 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index ad95e39..ecdfecd 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@v3 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 32eae92..43078e5 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v1 + uses: mshick/add-pr-comment@v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index 694e90e..e37cfda 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v7 + - uses: actions/stale@v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml new file mode 100644 index 0000000..8611458 --- /dev/null +++ b/.github/workflows/download_pipeline.yml @@ -0,0 +1,67 @@ +name: Test successful pipeline download with 'nf-core download' + +# Run the workflow when: +# - dispatched manually +# - when a PR is opened or reopened to master branch +# - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. +on: + workflow_dispatch: + pull_request: + types: + - opened + branches: + - master + pull_request_target: + branches: + - master + +env: + NXF_ANSI_LOG: false + +jobs: + download: + runs-on: ubuntu-latest + steps: + - name: Install Nextflow + uses: nf-core/setup-nextflow@v1 + + - uses: actions/setup-python@v5 + with: + python-version: "3.11" + architecture: "x64" + - uses: eWaterCycle/setup-singularity@v7 + with: + singularity-version: 3.8.3 + + - name: Install dependencies + run: | + python -m pip install --upgrade pip + pip install git+https://github.com/nf-core/tools.git@dev + + - name: Get the repository name and current branch set as environment variable + run: | + echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} + echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + + - name: Download the pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + run: | + nf-core download ${{ env.REPO_LOWERCASE }} \ + --revision ${{ env.REPO_BRANCH }} \ + --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ + --compress "none" \ + --container-system 'singularity' \ + --container-library "quay.io" -l "docker.io" -l "ghcr.io" \ + --container-cache-utilisation 'amend' \ + --download-configuration + + - name: Inspect download + run: tree ./${{ env.REPOTITLE_LOWERCASE }} + + - name: Run the downloaded pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 6286d83..06cc19a 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -4,7 +4,7 @@ on: types: [created] jobs: - deploy: + fix-linting: # Only run if comment is on a PR with the main repo, and if it contains the magic keywords if: > contains(github.event.comment.html_url, '/pull/') && @@ -13,10 +13,17 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 with: token: ${{ secrets.nf_core_bot_auth_token }} + # indication that the linting is being fixed + - name: React on comment + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: eyes + # Action runs on the issue comment, so we don't get the PR by default # Use the gh cli to check out the PR - name: Checkout Pull Request @@ -24,32 +31,59 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v4 + # Install and run pre-commit + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + with: + python-version: 3.11 - - name: Install Prettier - run: npm install -g prettier @prettier/plugin-php + - name: Install pre-commit + run: pip install pre-commit - # Check that we actually need to fix something - - name: Run 'prettier --check' - id: prettier_status - run: | - if prettier --check ${GITHUB_WORKSPACE}; then - echo "result=pass" >> $GITHUB_OUTPUT - else - echo "result=fail" >> $GITHUB_OUTPUT - fi + - name: Run pre-commit + id: pre-commit + run: pre-commit run --all-files + continue-on-error: true - - name: Run 'prettier --write' - if: steps.prettier_status.outputs.result == 'fail' - run: prettier --write ${GITHUB_WORKSPACE} + # indication that the linting has finished + - name: react if linting finished succesfully + if: steps.pre-commit.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: "+1" - name: Commit & push changes - if: steps.prettier_status.outputs.result == 'fail' + id: commit-and-push + if: steps.pre-commit.outcome == 'failure' run: | git config user.email "core@nf-co.re" git config user.name "nf-core-bot" git config push.default upstream git add . git status - git commit -m "[automated] Fix linting with Prettier" + git commit -m "[automated] Fix code linting" git push + + - name: react if linting errors were fixed + id: react-if-fixed + if: steps.commit-and-push.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: hooray + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: confused + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + issue-number: ${{ github.event.issue.number }} + body: | + @${{ github.actor }} I tried to fix the linting errors, but it didn't work. Please fix them manually. + See [CI log](https://github.com/nf-core/rangeland/actions/runs/${{ github.run_id }}) for more details. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 905c58e..81cd098 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,61 +11,22 @@ on: types: [published] jobs: - EditorConfig: + pre-commit: runs-on: ubuntu-latest steps: - uses: actions/checkout@v4 - - uses: actions/setup-node@v4 - - - name: Install editorconfig-checker - run: npm install -g editorconfig-checker - - - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') - - Prettier: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - uses: actions/setup-node@v4 - - - name: Install Prettier - run: npm install -g prettier - - - name: Run Prettier --check - run: prettier --check ${GITHUB_WORKSPACE} - - PythonBlack: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - name: Check code lints with Black - uses: psf/black@stable - - # If the above check failed, post a comment on the PR explaining the failure - - name: Post PR comment - if: failure() - uses: mshick/add-pr-comment@v1 + - name: Set up Python 3.11 + uses: actions/setup-python@v5 with: - message: | - ## Python linting (`black`) is failing - - To keep the code consistent with lots of contributors, we run automated code consistency checks. - To fix this CI test, please run: - - * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` - * Fix formatting errors in your pipeline: `black .` - - Once you push these changes the test should pass, and you can hide this comment :+1: + python-version: 3.11 + cache: "pip" - We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + - name: Install pre-commit + run: pip install pre-commit - Thanks again for your contribution! - repo-token: ${{ secrets.GITHUB_TOKEN }} - allow-repeats: false + - name: Run pre-commit + run: pre-commit run --all-files nf-core: runs-on: ubuntu-latest @@ -76,7 +37,7 @@ jobs: - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.11" architecture: "x64" @@ -99,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v3 + uses: actions/upload-artifact@v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 0bbcd30..147bcd1 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v2 + uses: dawidd6/action-download-artifact@v3 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 6ad3392..21ac3f0 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -24,7 +24,7 @@ jobs: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +56,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.0.2 + - uses: zentered/bluesky-post-action@v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/.gitpod.yml b/.gitpod.yml index acf7269..363d5b1 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -7,6 +7,7 @@ tasks: - name: unset JAVA_TOOL_OPTIONS command: | unset JAVA_TOOL_OPTIONS + vscode: extensions: # based on nf-core.nf-core-extensionpack - codezombiech.gitignore # Language support for .gitignore files diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 0c31cdb..af57081 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -1,5 +1,10 @@ repos: - repo: https://github.com/pre-commit/mirrors-prettier - rev: "v2.7.1" + rev: "v3.1.0" hooks: - id: prettier + - repo: https://github.com/editorconfig-checker/editorconfig-checker.python + rev: "2.7.3" + hooks: + - id: editorconfig-checker + alias: ec diff --git a/README.md b/README.md index 37605da..9c27025 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,9 @@ -# ![nf-core/rangeland](docs/images/nf-core-rangeland_logo_light.png#gh-light-mode-only) ![nf-core/rangeland](docs/images/nf-core-rangeland_logo_dark.png#gh-dark-mode-only) - +

+ + + nf-core/rangeland + +

[![GitHub Actions CI Status](https://github.com/nf-core/rangeland/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rangeland/actions?query=workflow%3A%22nf-core+CI%22) [![GitHub Actions Linting Status](https://github.com/nf-core/rangeland/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rangeland/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rangeland/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) diff --git a/assets/email_template.html b/assets/email_template.html index 53f8090..a566131 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -12,7 +12,7 @@ -

nf-core/rangeland v${version}

+

nf-core/rangeland ${version}

Run Name: $runName

<% if (!success){ diff --git a/assets/email_template.txt b/assets/email_template.txt index 575a487..cb9cb22 100644 --- a/assets/email_template.txt +++ b/assets/email_template.txt @@ -4,7 +4,7 @@ |\\ | |__ __ / ` / \\ |__) |__ } { | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, `._,._,' - nf-core/rangeland v${version} + nf-core/rangeland ${version} ---------------------------------------------------- Run Name: $runName diff --git a/assets/nf-core-rangeland_logo_light.png b/assets/nf-core-rangeland_logo_light.png index 118b325b6f9e5178ee939685add9331172b7befe..bb5b76e136d95253d5947538ecfd7053fe0d7271 100644 GIT binary patch literal 77297 zcmeEui93|*|NktQ%2p{POG2_GTh>fbF$vKkWvL`7S+eglWvLVyq^!vx5wgoR)sd*| zWoJr4wy}@>_kPs*ocb4j*Y~ijuVhOY*KysvQ|q$y*J-S zk72oMpYe0q?VAbaq#*9EMu$IdcoXp^so>_-$xz2=@*S~&`he~jv*V1qmOg7gB~|ku z4iN>q9{kdyZ|YpwtFN7@xuSZ(Q#H%X?NoYKwa=aXj}v2~+N64J89ds7jyopzTE&lL zTX=gIP6(U()S#j$694J$cS-q-%;cBHV%V6wo@O1Lq5Km$NZ4lj<2c zwqe?icigw3j2(aYEkjNIUZPcw=4bC$%q+7b?p1P^!$01``3%CuY^87 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config has been provided to run the pipeline NfcoreTemplate.checkConfigProvided(workflow, log) + // Check that the profile doesn't contain spaces and doesn't end with a trailing comma + checkProfile(workflow.profile, args, log) // Check that conda channels are set-up correctly if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { @@ -49,4 +51,16 @@ class WorkflowMain { Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") } } + + // + // Exit pipeline if --profile contains spaces + // + private static void checkProfile(profile, args, log) { + if (profile.endsWith(',')) { + Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } } diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar deleted file mode 100644 index 805c8bb5e4fd43a12a5891eea5a68788309629b0..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 2291171 zcma%i1CS<7mu}m(-Ea4_?dhK8v~AnAZQHhO+qP}n-E-&Lf8*Yb{bM(7MpZ;s){{|L znGpxiIY(9k6buar3JMCSNkdW|=)W{*ATS^)VMTsgF=-L{?{Od?P#{?eNT`1hApZ%I z{U0Zz{YCuW$x{5%Vj{u{igZ#U4^op8k`lCZbMO+hRMV3)4e|_&%)3YSbmCGpVp4NX zni_mKLGakt&P12{r^KCD0GPa9sIuy`fu+agt?8O-hY9i{WloGe}y@kSsDEY_Fud9 z-?2ab4J)MQXe4E3Xl-U<>PRMQZ)0m@@90KnV5#Te5Tz`pvZ0Lj4F<9%Flh(`Qb;Tn zMV!iFmH{S^fF*j#MCKRUd`7m>9||*;WE32I_6pg4-Q1C#ke$$aVmP-TtfJ+j+N9;Q z>bxN=bCVQkg$Jzty71(+~A9}ZfANuYIVnTdW!??Sj)Ti-Qpk8XgPDab8pk2QD&dwUl=iFhd1c$C2uMVagr zWc@z9s$#g__{3?VGb#)^fSNp^lZh zU>MI!D?+L)nyth{@@L0+B&_M8iR08L)G6#PZQLFmvz-9DlZkwRWigRIyI4xYr7>tRJ-N6MlBB`nJQ!!8D)mI2lB3PP;dC{<{Td>^ 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b/modules.json index 93e746a..8638ebe 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", + "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "4ab13872435962dadc239979554d13709e20bf29", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml index f0c63f6..9b3272b 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.17 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 7685b33..f218761 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -4,8 +4,8 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : - 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test index eec1db1..b1e1630 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -31,7 +31,12 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot( + process.out.versions, + file(process.out.mqc_yml[0]).readLines()[0..10], + file(process.out.yml[0]).readLines()[0..7] + ).match() + } ) } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap index 4274ed5..5f59a93 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -1,27 +1,33 @@ { "Should run without failures": { "content": [ - { - "0": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ], - "1": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "2": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "mqc_yml": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "versions": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "yml": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ] - } + [ + "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" + ], + [ + "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", + " \\n\\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n \\n \\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n\\n\\n \\n\\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\" + ], + [ + "CUSTOM_DUMPSOFTWAREVERSIONS:", + " python: 3.11.7", + " yaml: 5.4.1", + "TOOL1:", + " tool1: 0.11.9", + "TOOL2:", + " tool2: '1.9'", + "Workflow:" + ] ], - "timestamp": "2023-11-03T14:43:22.157011" + "timestamp": "2024-01-09T23:01:18.710682" } -} +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index b9e8f92..1f21c66 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -3,24 +3,20 @@ nextflow_process { name "Test Process FASTQC" script "../main.nf" process "FASTQC" + tag "modules" tag "modules_nfcore" tag "fastqc" - test("Single-Read") { + test("sarscov2 single-end [fastq]") { when { - params { - outdir = "$outputDir" - } process { """ - input[0] = [ + input[0] = Channel.of([ [ id: 'test', single_end:true ], - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) """ } } @@ -28,82 +24,189 @@ nextflow_process { then { assertAll ( { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. // looks like this:
Mon 2 Oct 2023
test.gz
// https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, - { assert path(process.out.html.get(0).get(1)).getText().contains("") }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 paired-end [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 interleaved [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } ) } } -// TODO -// // -// // Test with paired-end data -// // -// workflow test_fastqc_paired_end { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with interleaved data -// // -// workflow test_fastqc_interleaved { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with bam data -// // -// workflow test_fastqc_bam { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with multiple samples -// // -// workflow test_fastqc_multiple { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with custom prefix -// // -// workflow test_fastqc_custom_prefix { -// input = [ -// [ id:'mysample', single_end:true ], // meta map -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } + + test("sarscov2 paired-end [bam]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 multiple [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, + { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, + { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + { assert path(process.out.html[0][1][2]).text.contains("") }, + { assert path(process.out.html[0][1][3]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 custom_prefix") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastq] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.html.collect { file(it[1]).getName() } + + process.out.zip.collect { file(it[1]).getName() } + + process.out.versions ).match() } + ) + } + } + } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 636a32c..5d624bb 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,10 +1,20 @@ { + "sarscov2 single-end [fastq] - stub": { + "content": [ + [ + "test.html", + "test.zip", + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2024-01-17T18:40:57.254299" + }, "versions": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2023-10-09T23:40:54+0000" + "timestamp": "2024-01-17T18:36:50.033627" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index bc0bdb5..7625b75 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.18 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 00cc48d..1b9f7c4 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : - 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -43,7 +43,7 @@ process MULTIQC { stub: """ - touch multiqc_data + mkdir multiqc_data touch multiqc_plots touch multiqc_report.html diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f1aa660..45a9bc3 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,4 +1,3 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index c2dad21..d0438ed 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -7,12 +7,9 @@ nextflow_process { tag "modules_nfcore" tag "multiqc" - test("MULTIQC: FASTQC") { + test("sarscov2 single-end [fastqc]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -26,20 +23,17 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } ) } } - test("MULTIQC: FASTQC and a config file") { + test("sarscov2 single-end [fastqc] [config]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -53,9 +47,35 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastqc] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.report.collect { file(it).getName() } + + process.out.data.collect { file(it).getName() } + + process.out.plots.collect { file(it).getName() } + + process.out.versions ).match() } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap new file mode 100644 index 0000000..d37e730 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -0,0 +1,21 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:02:49.911994" + }, + "sarscov2 single-end [fastqc] - stub": { + "content": [ + [ + "multiqc_report.html", + "multiqc_data", + "multiqc_plots", + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:03:14.524346" + } +} \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index 5b0d857..dee8f20 100644 --- a/nextflow.config +++ b/nextflow.config @@ -67,7 +67,7 @@ try { } // Load nf-core/rangeland custom profiles from different institutions. -// Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs! +// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs! // try { // includeConfig "${params.custom_config_base}/pipeline/rangeland.config" // } catch (Exception e) { @@ -87,6 +87,7 @@ profiles { podman.enabled = false shifter.enabled = false charliecloud.enabled = false + channels = ['conda-forge', 'bioconda', 'defaults'] apptainer.enabled = false } mamba { diff --git a/pyproject.toml b/pyproject.toml index 0d62beb..7d08e1c 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,10 +1,13 @@ -# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. +# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.black] +[tool.ruff] line-length = 120 -target_version = ["py37", "py38", "py39", "py310"] +target-version = "py38" +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] +cache-dir = "~/.cache/ruff" -[tool.isort] -profile = "black" -known_first_party = ["nf_core"] -multi_line_output = 3 +[tool.ruff.isort] +known-first-party = ["nf_core"] + +[tool.ruff.per-file-ignores] +"__init__.py" = ["E402", "F401"] diff --git a/workflows/rangeland.nf b/workflows/rangeland.nf index f96104f..09192ea 100644 --- a/workflows/rangeland.nf +++ b/workflows/rangeland.nf @@ -127,6 +127,13 @@ workflow.onComplete { } } +workflow.onError { + if (workflow.errorReport.contains("Process requirement exceeds available memory")) { + println("🛑 Default resources exceed availability 🛑 ") + println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") + } +} + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ THE END From 51a4fa5d085f2d6ad186f0226b7bdaeba41e4e89 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 20 Feb 2024 15:30:06 +0000 Subject: [PATCH 06/61] Template update for nf-core/tools version 2.13 --- .editorconfig | 9 +- .github/workflows/awsfulltest.yml | 4 +- .github/workflows/awstest.yml | 4 +- .github/workflows/branch.yml | 2 +- .github/workflows/ci.yml | 7 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 17 +- .github/workflows/linting.yml | 12 +- .github/workflows/linting_comment.yml | 4 +- .github/workflows/release-announcements.yml | 11 +- README.md | 5 +- assets/multiqc_config.yml | 2 + assets/schema_input.json | 21 +- bin/check_samplesheet.py | 259 ----------- conf/modules.config | 8 - conf/test.config | 3 +- lib/NfcoreTemplate.groovy | 356 -------------- lib/Utils.groovy | 47 -- lib/WorkflowMain.groovy | 66 --- lib/WorkflowRangeland.groovy | 105 ----- main.nf | 88 ++-- modules.json | 28 +- modules/local/samplesheet_check.nf | 31 -- .../dumpsoftwareversions/environment.yml | 7 - .../custom/dumpsoftwareversions/main.nf | 24 - .../custom/dumpsoftwareversions/meta.yml | 37 -- .../templates/dumpsoftwareversions.py | 102 ---- .../dumpsoftwareversions/tests/main.nf.test | 43 -- .../tests/main.nf.test.snap | 33 -- .../dumpsoftwareversions/tests/tags.yml | 2 - modules/nf-core/fastqc/tests/main.nf.test | 14 +- .../nf-core/fastqc/tests/main.nf.test.snap | 76 ++- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- modules/nf-core/multiqc/tests/main.nf.test | 13 +- .../nf-core/multiqc/tests/main.nf.test.snap | 32 +- nextflow.config | 6 +- nextflow_schema.json | 22 +- pyproject.toml | 8 +- subworkflows/local/input_check.nf | 44 -- .../utils_nfcore_rangeland_pipeline/main.nf | 212 +++++++++ .../nf-core/utils_nextflow_pipeline/main.nf | 126 +++++ .../nf-core/utils_nextflow_pipeline/meta.yml | 38 ++ .../tests/main.function.nf.test | 54 +++ .../tests/main.function.nf.test.snap | 12 + .../tests/main.workflow.nf.test | 123 +++++ .../tests/nextflow.config | 9 + .../utils_nextflow_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfcore_pipeline/main.nf | 440 ++++++++++++++++++ .../nf-core/utils_nfcore_pipeline/meta.yml | 24 + .../tests/main.function.nf.test | 134 ++++++ .../tests/main.function.nf.test.snap | 138 ++++++ .../tests/main.workflow.nf.test | 29 ++ .../tests/main.workflow.nf.test.snap | 15 + .../tests/nextflow.config | 9 + .../utils_nfcore_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfvalidation_plugin/main.nf | 62 +++ .../utils_nfvalidation_plugin/meta.yml | 44 ++ .../tests/main.nf.test | 200 ++++++++ .../tests/nextflow_schema.json | 96 ++++ .../utils_nfvalidation_plugin/tests/tags.yml | 2 + workflows/rangeland.nf | 133 ++---- 62 files changed, 2067 insertions(+), 1397 deletions(-) delete mode 100755 bin/check_samplesheet.py delete mode 100755 lib/NfcoreTemplate.groovy delete mode 100644 lib/Utils.groovy delete mode 100755 lib/WorkflowMain.groovy delete mode 100755 lib/WorkflowRangeland.groovy delete mode 100644 modules/local/samplesheet_check.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml delete mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml delete mode 100644 subworkflows/local/input_check.nf create mode 100644 subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml diff --git a/.editorconfig b/.editorconfig index 9b99008..dd9ffa5 100644 --- a/.editorconfig +++ b/.editorconfig @@ -18,7 +18,12 @@ end_of_line = unset insert_final_newline = unset trim_trailing_whitespace = unset indent_style = unset -indent_size = unset +[/subworkflows/nf-core/**] +charset = unset +end_of_line = unset +insert_final_newline = unset +trim_trailing_whitespace = unset +indent_style = unset [/assets/email*] indent_size = unset @@ -28,5 +33,5 @@ indent_size = unset indent_style = unset # ignore python -[*.{py}] +[*.{py,md}] indent_style = unset diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 567c485..fd38d18 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v2 + uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@v4 + - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index ecdfecd..23daa39 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,7 +12,7 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v2 + uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@v4 + - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 43078e5..7ea70c0 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v2 + uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 226bce4..5b8d0d1 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,13 +28,16 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 with: version: "${{ matrix.NXF_VER }}" + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + - name: Run pipeline with test data # TODO nf-core: You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index e37cfda..0b6b1f2 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v9 + - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 8611458..f823210 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -6,6 +6,11 @@ name: Test successful pipeline download with 'nf-core download' # - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. on: workflow_dispatch: + inputs: + testbranch: + description: "The specific branch you wish to utilize for the test execution of nf-core download." + required: true + default: "dev" pull_request: types: - opened @@ -23,13 +28,13 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" - - uses: eWaterCycle/setup-singularity@v7 + - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 with: singularity-version: 3.8.3 @@ -42,13 +47,13 @@ jobs: run: | echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} - echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} - name: Download the pipeline env: NXF_SINGULARITY_CACHEDIR: ./ run: | - nf-core download ${{ env.REPO_LOWERCASE }} \ + nf-core download ${{ env.REPO_LOWERCASE }} \ --revision ${{ env.REPO_BRANCH }} \ --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ --compress "none" \ @@ -64,4 +69,4 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 81cd098..748b431 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,10 +14,10 @@ jobs: pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Set up Python 3.11 - uses: actions/setup-python@v5 + uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: 3.11 cache: "pip" @@ -32,12 +32,12 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" @@ -60,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v4 + uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 147bcd1..b706875 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v3 + uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 with: workflow: linting.yml workflow_conclusion: completed @@ -21,7 +21,7 @@ jobs: run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment - uses: marocchino/sticky-pull-request-comment@v2 + uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2 with: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} number: ${{ steps.pr_number.outputs.pr_number }} diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 21ac3f0..c3674af 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -9,6 +9,11 @@ jobs: toot: runs-on: ubuntu-latest steps: + - name: get topics and convert to hashtags + id: get_topics + run: | + curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' > $GITHUB_OUTPUT + - uses: rzr/fediverse-action@master with: access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} @@ -20,11 +25,13 @@ jobs: Please see the changelog: ${{ github.event.release.html_url }} + ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics + send-tweet: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +63,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.1.0 + - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/README.md b/README.md index 9c27025..c925d65 100644 --- a/README.md +++ b/README.md @@ -4,8 +4,9 @@ nf-core/rangeland -[![GitHub Actions CI Status](https://github.com/nf-core/rangeland/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rangeland/actions?query=workflow%3A%22nf-core+CI%22) -[![GitHub Actions Linting Status](https://github.com/nf-core/rangeland/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rangeland/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rangeland/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) + +[![GitHub Actions CI Status](https://github.com/nf-core/rangeland/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rangeland/actions/workflows/ci.yml) +[![GitHub Actions Linting Status](https://github.com/nf-core/rangeland/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rangeland/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rangeland/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 2d0c6ee..d2ae102 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -11,3 +11,5 @@ report_section_order: order: -1002 export_plots: true + +disable_version_detection: true diff --git a/assets/schema_input.json b/assets/schema_input.json index 655729c..8c4e376 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -10,25 +10,22 @@ "sample": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Sample name must be provided and cannot contain spaces" + "errorMessage": "Sample name must be provided and cannot contain spaces", + "meta": ["id"] }, "fastq_1": { "type": "string", + "format": "file-path", + "exists": true, "pattern": "^\\S+\\.f(ast)?q\\.gz$", "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" }, "fastq_2": { - "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", - "anyOf": [ - { - "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$" - }, - { - "type": "string", - "maxLength": 0 - } - ] + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.f(ast)?q\\.gz$", + "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" } }, "required": ["sample", "fastq_1"] diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py deleted file mode 100755 index 4a758fe..0000000 --- a/bin/check_samplesheet.py +++ /dev/null @@ -1,259 +0,0 @@ -#!/usr/bin/env python - - -"""Provide a command line tool to validate and transform tabular samplesheets.""" - - -import argparse -import csv -import logging -import sys -from collections import Counter -from pathlib import Path - -logger = logging.getLogger() - - -class RowChecker: - """ - Define a service that can validate and transform each given row. - - Attributes: - modified (list): A list of dicts, where each dict corresponds to a previously - validated and transformed row. The order of rows is maintained. - - """ - - VALID_FORMATS = ( - ".fq.gz", - ".fastq.gz", - ) - - def __init__( - self, - sample_col="sample", - first_col="fastq_1", - second_col="fastq_2", - single_col="single_end", - **kwargs, - ): - """ - Initialize the row checker with the expected column names. - - Args: - sample_col (str): The name of the column that contains the sample name - (default "sample"). - first_col (str): The name of the column that contains the first (or only) - FASTQ file path (default "fastq_1"). - second_col (str): The name of the column that contains the second (if any) - FASTQ file path (default "fastq_2"). - single_col (str): The name of the new column that will be inserted and - records whether the sample contains single- or paired-end sequencing - reads (default "single_end"). - - """ - super().__init__(**kwargs) - self._sample_col = sample_col - self._first_col = first_col - self._second_col = second_col - self._single_col = single_col - self._seen = set() - self.modified = [] - - def validate_and_transform(self, row): - """ - Perform all validations on the given row and insert the read pairing status. - - Args: - row (dict): A mapping from column headers (keys) to elements of that row - (values). - - """ - self._validate_sample(row) - self._validate_first(row) - self._validate_second(row) - self._validate_pair(row) - self._seen.add((row[self._sample_col], row[self._first_col])) - self.modified.append(row) - - def _validate_sample(self, row): - """Assert that the sample name exists and convert spaces to underscores.""" - if len(row[self._sample_col]) <= 0: - raise AssertionError("Sample input is required.") - # Sanitize samples slightly. - row[self._sample_col] = row[self._sample_col].replace(" ", "_") - - def _validate_first(self, row): - """Assert that the first FASTQ entry is non-empty and has the right format.""" - if len(row[self._first_col]) <= 0: - raise AssertionError("At least the first FASTQ file is required.") - self._validate_fastq_format(row[self._first_col]) - - def _validate_second(self, row): - """Assert that the second FASTQ entry has the right format if it exists.""" - if len(row[self._second_col]) > 0: - self._validate_fastq_format(row[self._second_col]) - - def _validate_pair(self, row): - """Assert that read pairs have the same file extension. Report pair status.""" - if row[self._first_col] and row[self._second_col]: - row[self._single_col] = False - first_col_suffix = Path(row[self._first_col]).suffixes[-2:] - second_col_suffix = Path(row[self._second_col]).suffixes[-2:] - if first_col_suffix != second_col_suffix: - raise AssertionError("FASTQ pairs must have the same file extensions.") - else: - row[self._single_col] = True - - def _validate_fastq_format(self, filename): - """Assert that a given filename has one of the expected FASTQ extensions.""" - if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): - raise AssertionError( - f"The FASTQ file has an unrecognized extension: {filename}\n" - f"It should be one of: {', '.join(self.VALID_FORMATS)}" - ) - - def validate_unique_samples(self): - """ - Assert that the combination of sample name and FASTQ filename is unique. - - In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the - number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. - - """ - if len(self._seen) != len(self.modified): - raise AssertionError("The pair of sample name and FASTQ must be unique.") - seen = Counter() - for row in self.modified: - sample = row[self._sample_col] - seen[sample] += 1 - row[self._sample_col] = f"{sample}_T{seen[sample]}" - - -def read_head(handle, num_lines=10): - """Read the specified number of lines from the current position in the file.""" - lines = [] - for idx, line in enumerate(handle): - if idx == num_lines: - break - lines.append(line) - return "".join(lines) - - -def sniff_format(handle): - """ - Detect the tabular format. - - Args: - handle (text file): A handle to a `text file`_ object. The read position is - expected to be at the beginning (index 0). - - Returns: - csv.Dialect: The detected tabular format. - - .. _text file: - https://docs.python.org/3/glossary.html#term-text-file - - """ - peek = read_head(handle) - handle.seek(0) - sniffer = csv.Sniffer() - dialect = sniffer.sniff(peek) - return dialect - - -def check_samplesheet(file_in, file_out): - """ - Check that the tabular samplesheet has the structure expected by nf-core pipelines. - - Validate the general shape of the table, expected columns, and each row. Also add - an additional column which records whether one or two FASTQ reads were found. - - Args: - file_in (pathlib.Path): The given tabular samplesheet. The format can be either - CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. - file_out (pathlib.Path): Where the validated and transformed samplesheet should - be created; always in CSV format. - - Example: - This function checks that the samplesheet follows the following structure, - see also the `viral recon samplesheet`_:: - - sample,fastq_1,fastq_2 - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, - - .. _viral recon samplesheet: - https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv - - """ - required_columns = {"sample", "fastq_1", "fastq_2"} - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_in.open(newline="") as in_handle: - reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) - # Validate the existence of the expected header columns. - if not required_columns.issubset(reader.fieldnames): - req_cols = ", ".join(required_columns) - logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") - sys.exit(1) - # Validate each row. - checker = RowChecker() - for i, row in enumerate(reader): - try: - checker.validate_and_transform(row) - except AssertionError as error: - logger.critical(f"{str(error)} On line {i + 2}.") - sys.exit(1) - checker.validate_unique_samples() - header = list(reader.fieldnames) - header.insert(1, "single_end") - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_out.open(mode="w", newline="") as out_handle: - writer = csv.DictWriter(out_handle, header, delimiter=",") - writer.writeheader() - for row in checker.modified: - writer.writerow(row) - - -def parse_args(argv=None): - """Define and immediately parse command line arguments.""" - parser = argparse.ArgumentParser( - description="Validate and transform a tabular samplesheet.", - epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", - ) - parser.add_argument( - "file_in", - metavar="FILE_IN", - type=Path, - help="Tabular input samplesheet in CSV or TSV format.", - ) - parser.add_argument( - "file_out", - metavar="FILE_OUT", - type=Path, - help="Transformed output samplesheet in CSV format.", - ) - parser.add_argument( - "-l", - "--log-level", - help="The desired log level (default WARNING).", - choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), - default="WARNING", - ) - return parser.parse_args(argv) - - -def main(argv=None): - """Coordinate argument parsing and program execution.""" - args = parse_args(argv) - logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") - if not args.file_in.is_file(): - logger.error(f"The given input file {args.file_in} was not found!") - sys.exit(2) - args.file_out.parent.mkdir(parents=True, exist_ok=True) - check_samplesheet(args.file_in, args.file_out) - - -if __name__ == "__main__": - sys.exit(main()) diff --git a/conf/modules.config b/conf/modules.config index d91c6ab..e3ea8fa 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -18,14 +18,6 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: SAMPLESHEET_CHECK { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - withName: FASTQC { ext.args = '--quiet' } diff --git a/conf/test.config b/conf/test.config index 9ab2465..0a3709b 100644 --- a/conf/test.config +++ b/conf/test.config @@ -24,6 +24,5 @@ params { // TODO nf-core: Give any required params for the test so that command line flags are not needed input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' - // Fasta references - fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/genome/NC_045512.2/GCF_009858895.2_ASM985889v3_genomic.200409.fna.gz' + } diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy deleted file mode 100755 index e248e4c..0000000 --- a/lib/NfcoreTemplate.groovy +++ /dev/null @@ -1,356 +0,0 @@ -// -// This file holds several functions used within the nf-core pipeline template. -// - -import org.yaml.snakeyaml.Yaml -import groovy.json.JsonOutput -import nextflow.extension.FilesEx - -class NfcoreTemplate { - - // - // Check AWS Batch related parameters have been specified correctly - // - public static void awsBatch(workflow, params) { - if (workflow.profile.contains('awsbatch')) { - // Check params.awsqueue and params.awsregion have been set if running on AWSBatch - assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - } - } - - // - // Warn if a -profile or Nextflow config has not been provided to run the pipeline - // - public static void checkConfigProvided(workflow, log) { - if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " - } - } - - // - // Generate version string - // - public static String version(workflow) { - String version_string = "" - - if (workflow.manifest.version) { - def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' - version_string += "${prefix_v}${workflow.manifest.version}" - } - - if (workflow.commitId) { - def git_shortsha = workflow.commitId.substring(0, 7) - version_string += "-g${git_shortsha}" - } - - return version_string - } - - // - // Construct and send completion email - // - public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['Date Started'] = workflow.start - misc_fields['Date Completed'] = workflow.complete - misc_fields['Pipeline script file path'] = workflow.scriptFile - misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build - misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - def email_fields = [:] - email_fields['version'] = NfcoreTemplate.version(workflow) - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary << misc_fields - - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { - if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" - } - mqc_report = mqc_report[0] - } - } - } catch (all) { - if (multiqc_report) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" - } - } - - // Check if we are only sending emails on failure - def email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - Map colors = logColours(params.monochrome_logs) - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") - sendmail_tf.withWriter { w -> w << sendmail_html } - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" - } - } - - // Write summary e-mail HTML to a file - def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); - output_hf.delete() - - // Write summary e-mail TXT to a file - def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); - output_tf.delete() - } - - // - // Construct and send a notification to a web server as JSON - // e.g. Microsoft Teams and Slack - // - public static void IM_notification(workflow, params, summary_params, projectDir, log) { - def hook_url = params.hook_url - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp - - def msg_fields = [:] - msg_fields['version'] = NfcoreTemplate.version(workflow) - msg_fields['runName'] = workflow.runName - msg_fields['success'] = workflow.success - msg_fields['dateComplete'] = workflow.complete - msg_fields['duration'] = workflow.duration - msg_fields['exitStatus'] = workflow.exitStatus - msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - msg_fields['errorReport'] = (workflow.errorReport ?: 'None') - msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") - msg_fields['projectDir'] = workflow.projectDir - msg_fields['summary'] = summary << misc_fields - - // Render the JSON template - def engine = new groovy.text.GStringTemplateEngine() - // Different JSON depending on the service provider - // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format - def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" - def hf = new File("$projectDir/assets/${json_path}") - def json_template = engine.createTemplate(hf).make(msg_fields) - def json_message = json_template.toString() - - // POST - def post = new URL(hook_url).openConnection(); - post.setRequestMethod("POST") - post.setDoOutput(true) - post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); - } - } - - // - // Dump pipeline parameters in a json file - // - public static void dump_parameters(workflow, params) { - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def filename = "params_${timestamp}.json" - def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = JsonOutput.toJson(params) - temp_pf.text = JsonOutput.prettyPrint(jsonStr) - - FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") - temp_pf.delete() - } - - // - // Print pipeline summary on completion - // - public static void summary(workflow, params, log) { - Map colors = logColours(params.monochrome_logs) - if (workflow.success) { - if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" - } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" - } - } - - // - // ANSII Colours used for terminal logging - // - public static Map logColours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - // - // Does what is says on the tin - // - public static String dashedLine(monochrome_logs) { - Map colors = logColours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - // - // nf-core logo - // - public static String logo(workflow, monochrome_logs) { - Map colors = logColours(monochrome_logs) - String workflow_version = NfcoreTemplate.version(workflow) - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/lib/Utils.groovy b/lib/Utils.groovy deleted file mode 100644 index 8d030f4..0000000 --- a/lib/Utils.groovy +++ /dev/null @@ -1,47 +0,0 @@ -// -// This file holds several Groovy functions that could be useful for any Nextflow pipeline -// - -import org.yaml.snakeyaml.Yaml - -class Utils { - - // - // When running with -profile conda, warn if channels have not been set-up appropriately - // - public static void checkCondaChannels(log) { - Yaml parser = new Yaml() - def channels = [] - try { - def config = parser.load("conda config --show channels".execute().text) - channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return - } - - // Check that all channels are present - // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] - def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean - - // Check that they are in the right order - def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) - } - - if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - } - } -} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy deleted file mode 100755 index 47ff343..0000000 --- a/lib/WorkflowMain.groovy +++ /dev/null @@ -1,66 +0,0 @@ -// -// This file holds several functions specific to the main.nf workflow in the nf-core/rangeland pipeline -// - -import nextflow.Nextflow - -class WorkflowMain { - - // - // Citation string for pipeline - // - public static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - // TODO nf-core: Add Zenodo DOI for pipeline after first release - //"* The pipeline\n" + - //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" - } - - - // - // Validate parameters and print summary to screen - // - public static void initialise(workflow, params, log, args) { - - // Print workflow version and exit on --version - if (params.version) { - String workflow_version = NfcoreTemplate.version(workflow) - log.info "${workflow.manifest.name} ${workflow_version}" - System.exit(0) - } - - // Check that a -profile or Nextflow config has been provided to run the pipeline - NfcoreTemplate.checkConfigProvided(workflow, log) - // Check that the profile doesn't contain spaces and doesn't end with a trailing comma - checkProfile(workflow.profile, args, log) - - // Check that conda channels are set-up correctly - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - Utils.checkCondaChannels(log) - } - - // Check AWS batch settings - NfcoreTemplate.awsBatch(workflow, params) - - // Check input has been provided - if (!params.input) { - Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") - } - } - - // - // Exit pipeline if --profile contains spaces - // - private static void checkProfile(profile, args, log) { - if (profile.endsWith(',')) { - Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - if (args[0]) { - log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - } -} diff --git a/lib/WorkflowRangeland.groovy b/lib/WorkflowRangeland.groovy deleted file mode 100755 index 70f7c1b..0000000 --- a/lib/WorkflowRangeland.groovy +++ /dev/null @@ -1,105 +0,0 @@ -// -// This file holds several functions specific to the workflow/rangeland.nf in the nf-core/rangeland pipeline -// - -import nextflow.Nextflow -import groovy.text.SimpleTemplateEngine - -class WorkflowRangeland { - - // - // Check and validate parameters - // - public static void initialise(params, log) { - - - if (!params.fasta) { - Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - } - } - - // - // Get workflow summary for MultiQC - // - public static String paramsSummaryMultiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

$group

\n" - summary_section += "
\n" - for (param in group_params.keySet()) { - summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" - } - summary_section += "
\n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } - - // - // Generate methods description for MultiQC - // - - public static String toolCitationText(params) { - - // TODO nf-core: Optionally add in-text citation tools to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def citation_text = [ - "Tools used in the workflow included:", - "FastQC (Andrews 2010),", - "MultiQC (Ewels et al. 2016)", - "." - ].join(' ').trim() - - return citation_text - } - - public static String toolBibliographyText(params) { - - // TODO Optionally add bibliographic entries to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " - ].join(' ').trim() - - return reference_text - } - - public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { - // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file - def meta = [:] - meta.workflow = run_workflow.toMap() - meta["manifest_map"] = run_workflow.manifest.toMap() - - // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " - - // Tool references - meta["tool_citations"] = "" - meta["tool_bibliography"] = "" - - // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - //meta["tool_bibliography"] = toolBibliographyText(params) - - - def methods_text = mqc_methods_yaml.text - - def engine = new SimpleTemplateEngine() - def description_html = engine.createTemplate(methods_text).make(meta) - - return description_html - }} diff --git a/main.nf b/main.nf index 46cc501..9792f74 100644 --- a/main.nf +++ b/main.nf @@ -13,55 +13,83 @@ nextflow.enable.dsl = 2 /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - VALIDATE & PRINT PARAMETER SUMMARY + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { validateParameters; paramsHelp } from 'plugin/nf-validation' - -// Print help message if needed -if (params.help) { - def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) - def citation = '\n' + WorkflowMain.citation(workflow) + '\n' - def String command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" - log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs) - System.exit(0) -} - -// Validate input parameters -if (params.validate_params) { - validateParameters() -} - -WorkflowMain.initialise(workflow, params, log, args) +include { RANGELAND } from './workflows/rangeland' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_rangeland_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_rangeland_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - NAMED WORKFLOW FOR PIPELINE + NAMED WORKFLOWS FOR PIPELINE ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { RANGELAND } from './workflows/rangeland' - // -// WORKFLOW: Run main nf-core/rangeland analysis pipeline +// WORKFLOW: Run main analysis pipeline depending on type of input // workflow NFCORE_RANGELAND { - RANGELAND () -} + take: + samplesheet // channel: samplesheet read in from --input + + main: + + // + // WORKFLOW: Run pipeline + // + RANGELAND ( + samplesheet + ) + + emit: + multiqc_report = RANGELAND.out.multiqc_report // channel: /path/to/multiqc_report.html + +} /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - RUN ALL WORKFLOWS + RUN MAIN WORKFLOW ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// -// WORKFLOW: Execute a single named workflow for the pipeline -// See: https://github.com/nf-core/rnaseq/issues/619 -// workflow { - NFCORE_RANGELAND () + + main: + + // + // SUBWORKFLOW: Run initialisation tasks + // + PIPELINE_INITIALISATION ( + params.version, + params.help, + params.validate_params, + params.monochrome_logs, + args, + params.outdir, + params.input + ) + + // + // WORKFLOW: Run main workflow + // + NFCORE_RANGELAND ( + PIPELINE_INITIALISATION.out.samplesheet + ) + + // + // SUBWORKFLOW: Run completion tasks + // + PIPELINE_COMPLETION ( + params.email, + params.email_on_fail, + params.plaintext_email, + params.outdir, + params.monochrome_logs, + params.hook_url, + NFCORE_RANGELAND.out.multiqc_report + ) } /* diff --git a/modules.json b/modules.json index 8638ebe..68efc57 100644 --- a/modules.json +++ b/modules.json @@ -5,22 +5,36 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] - }, "fastqc": { "branch": "master", - "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", + "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", + "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290", "installed_by": ["modules"] } } + }, + "subworkflows": { + "nf-core": { + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "installed_by": ["subworkflows"] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + } + } } } } diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf deleted file mode 100644 index 44fe7f9..0000000 --- a/modules/local/samplesheet_check.nf +++ /dev/null @@ -1,31 +0,0 @@ -process SAMPLESHEET_CHECK { - tag "$samplesheet" - label 'process_single' - - conda "conda-forge::python=3.8.3" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'biocontainers/python:3.8.3' }" - - input: - path samplesheet - - output: - path '*.csv' , emit: csv - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: // This script is bundled with the pipeline, in nf-core/rangeland/bin/ - """ - check_samplesheet.py \\ - $samplesheet \\ - samplesheet.valid.csv - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | sed 's/Python //g') - END_VERSIONS - """ -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml deleted file mode 100644 index 9b3272b..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: custom_dumpsoftwareversions -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf deleted file mode 100644 index f218761..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ /dev/null @@ -1,24 +0,0 @@ -process CUSTOM_DUMPSOFTWAREVERSIONS { - label 'process_single' - - // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" - - input: - path versions - - output: - path "software_versions.yml" , emit: yml - path "software_versions_mqc.yml", emit: mqc_yml - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - template 'dumpsoftwareversions.py' -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml deleted file mode 100644 index 5f15a5f..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ /dev/null @@ -1,37 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: custom_dumpsoftwareversions -description: Custom module used to dump software versions within the nf-core pipeline template -keywords: - - custom - - dump - - version -tools: - - custom: - description: Custom module used to dump software versions within the nf-core pipeline template - homepage: https://github.com/nf-core/tools - documentation: https://github.com/nf-core/tools - licence: ["MIT"] -input: - - versions: - type: file - description: YML file containing software versions - pattern: "*.yml" -output: - - yml: - type: file - description: Standard YML file containing software versions - pattern: "software_versions.yml" - - mqc_yml: - type: file - description: MultiQC custom content YML file containing software versions - pattern: "software_versions_mqc.yml" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" -maintainers: - - "@drpatelh" - - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py deleted file mode 100755 index e55b8d4..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ /dev/null @@ -1,102 +0,0 @@ -#!/usr/bin/env python - - -"""Provide functions to merge multiple versions.yml files.""" - - -import platform -from textwrap import dedent - -import yaml - - -def _make_versions_html(versions): - """Generate a tabular HTML output of all versions for MultiQC.""" - html = [ - dedent( - """\\ - -
    Process Name \\", + " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    - - - - - - - - """ - ) - ] - for process, tmp_versions in sorted(versions.items()): - html.append("") - for i, (tool, version) in enumerate(sorted(tmp_versions.items())): - html.append( - dedent( - f"""\\ - - - - - - """ - ) - ) - html.append("") - html.append("
    Process Name Software Version
    {process if (i == 0) else ''}{tool}{version}
    ") - return "\\n".join(html) - - -def main(): - """Load all version files and generate merged output.""" - versions_this_module = {} - versions_this_module["${task.process}"] = { - "python": platform.python_version(), - "yaml": yaml.__version__, - } - - with open("$versions") as f: - versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module - - # aggregate versions by the module name (derived from fully-qualified process name) - versions_by_module = {} - for process, process_versions in versions_by_process.items(): - module = process.split(":")[-1] - try: - if versions_by_module[module] != process_versions: - raise AssertionError( - "We assume that software versions are the same between all modules. " - "If you see this error-message it means you discovered an edge-case " - "and should open an issue in nf-core/tools. " - ) - except KeyError: - versions_by_module[module] = process_versions - - versions_by_module["Workflow"] = { - "Nextflow": "$workflow.nextflow.version", - "$workflow.manifest.name": "$workflow.manifest.version", - } - - versions_mqc = { - "id": "software_versions", - "section_name": "${workflow.manifest.name} Software Versions", - "section_href": "https://github.com/${workflow.manifest.name}", - "plot_type": "html", - "description": "are collected at run time from the software output.", - "data": _make_versions_html(versions_by_module), - } - - with open("software_versions.yml", "w") as f: - yaml.dump(versions_by_module, f, default_flow_style=False) - with open("software_versions_mqc.yml", "w") as f: - yaml.dump(versions_mqc, f, default_flow_style=False) - - with open("versions.yml", "w") as f: - yaml.dump(versions_this_module, f, default_flow_style=False) - - -if __name__ == "__main__": - main() diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test deleted file mode 100644 index b1e1630..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ /dev/null @@ -1,43 +0,0 @@ -nextflow_process { - - name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" - script "../main.nf" - process "CUSTOM_DUMPSOFTWAREVERSIONS" - tag "modules" - tag "modules_nfcore" - tag "custom" - tag "dumpsoftwareversions" - tag "custom/dumpsoftwareversions" - - test("Should run without failures") { - when { - process { - """ - def tool1_version = ''' - TOOL1: - tool1: 0.11.9 - '''.stripIndent() - - def tool2_version = ''' - TOOL2: - tool2: 1.9 - '''.stripIndent() - - input[0] = Channel.of(tool1_version, tool2_version).collectFile() - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - process.out.versions, - file(process.out.mqc_yml[0]).readLines()[0..10], - file(process.out.yml[0]).readLines()[0..7] - ).match() - } - ) - } - } -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap deleted file mode 100644 index 5f59a93..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ /dev/null @@ -1,33 +0,0 @@ -{ - "Should run without failures": { - "content": [ - [ - "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" - ], - [ - "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", - " \\n\\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n \\n \\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n\\n\\n \\n\\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\" - ], - [ - "CUSTOM_DUMPSOFTWAREVERSIONS:", - " python: 3.11.7", - " yaml: 5.4.1", - "TOOL1:", - " tool1: 0.11.9", - "TOOL2:", - " tool2: '1.9'", - "Workflow:" - ] - ], - "timestamp": "2024-01-09T23:01:18.710682" - } -} \ No newline at end of file diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml deleted file mode 100644 index 405aa24..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -custom/dumpsoftwareversions: - - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 1f21c66..70edae4 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -33,7 +33,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_single") } ) } } @@ -63,7 +63,7 @@ nextflow_process { { assert path(process.out.html[0][1][0]).text.contains("") }, { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_paired") } ) } } @@ -89,7 +89,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } ) } } @@ -115,7 +115,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_bam") } ) } } @@ -153,7 +153,7 @@ nextflow_process { { assert path(process.out.html[0][1][2]).text.contains("") }, { assert path(process.out.html[0][1][3]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } ) } } @@ -179,7 +179,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } ) } } @@ -204,7 +204,7 @@ nextflow_process { { assert process.success }, { assert snapshot(process.out.html.collect { file(it[1]).getName() } + process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match() } + process.out.versions ).match("fastqc_stub") } ) } } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 5d624bb..86f7c31 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,5 +1,17 @@ { - "sarscov2 single-end [fastq] - stub": { + "fastqc_versions_interleaved": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:07.293713" + }, + "fastqc_stub": { "content": [ [ "test.html", @@ -7,14 +19,70 @@ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:40:57.254299" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:31:01.425198" + }, + "fastqc_versions_multiple": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:55.797907" + }, + "fastqc_versions_bam": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:26.795862" + }, + "fastqc_versions_single": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:27.043675" + }, + "fastqc_versions_paired": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:47.584191" }, - "versions": { + "fastqc_versions_custom_prefix": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:36:50.033627" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:41:14.576531" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 7625b75..2212096 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.19 + - bioconda::multiqc=1.20 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1b9f7c4..354f443 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : + 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index d0438ed..f1c4242 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -3,6 +3,7 @@ nextflow_process { name "Test Process MULTIQC" script "../main.nf" process "MULTIQC" + tag "modules" tag "modules_nfcore" tag "multiqc" @@ -12,7 +13,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -25,7 +26,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_single") } ) } @@ -36,7 +37,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) input[2] = [] input[3] = [] @@ -49,7 +50,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_config") } ) } } @@ -61,7 +62,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -75,7 +76,7 @@ nextflow_process { { assert snapshot(process.out.report.collect { file(it).getName() } + process.out.data.collect { file(it).getName() } + process.out.plots.collect { file(it).getName() } + - process.out.versions ).match() } + process.out.versions ).match("multiqc_stub") } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index d37e730..c204b48 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -1,21 +1,41 @@ { - "versions": { + "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" ] ], - "timestamp": "2024-01-09T23:02:49.911994" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:28:51.744211298" }, - "sarscov2 single-end [fastqc] - stub": { + "multiqc_stub": { "content": [ [ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" ] ], - "timestamp": "2024-01-09T23:03:14.524346" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:29:28.847433492" + }, + "multiqc_versions_config": { + "content": [ + [ + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:29:13.223621555" } } \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index dee8f20..aac9a2b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -11,10 +11,7 @@ params { // TODO nf-core: Specify your pipeline's command line flags // Input options - input = null - fasta = null - - // MultiQC options + input = null// MultiQC options multiqc_config = null multiqc_title = null multiqc_logo = null @@ -39,7 +36,6 @@ params { custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null - // Max resource options // Defaults only, expecting to be overwritten diff --git a/nextflow_schema.json b/nextflow_schema.json index b418065..9d71dd1 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -16,6 +16,7 @@ "type": "string", "format": "file-path", "exists": true, + "schema": "assets/schema_input.json", "mimetype": "text/csv", "pattern": "^\\S+\\.csv$", "description": "Path to comma-separated file containing information about the samples in the experiment.", @@ -42,24 +43,6 @@ } } }, - "reference_genome_options": { - "title": "Reference genome options", - "type": "object", - "fa_icon": "fas fa-dna", - "description": "Reference genome related files and options required for the workflow.", - "properties": { - "fasta": { - "type": "string", - "format": "file-path", - "exists": true, - "mimetype": "text/plain", - "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", - "description": "Path to FASTA genome file.", - "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", - "fa_icon": "far fa-file-code" - } - } - }, "institutional_config_options": { "title": "Institutional config options", "type": "object", @@ -259,9 +242,6 @@ { "$ref": "#/definitions/input_output_options" }, - { - "$ref": "#/definitions/reference_genome_options" - }, { "$ref": "#/definitions/institutional_config_options" }, diff --git a/pyproject.toml b/pyproject.toml index 7d08e1c..5611062 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -3,11 +3,13 @@ [tool.ruff] line-length = 120 target-version = "py38" -select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] cache-dir = "~/.cache/ruff" -[tool.ruff.isort] +[tool.ruff.lint] +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] + +[tool.ruff.lint.isort] known-first-party = ["nf_core"] -[tool.ruff.per-file-ignores] +[tool.ruff.lint.per-file-ignores] "__init__.py" = ["E402", "F401"] diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf deleted file mode 100644 index 0aecf87..0000000 --- a/subworkflows/local/input_check.nf +++ /dev/null @@ -1,44 +0,0 @@ -// -// Check input samplesheet and get read channels -// - -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' - -workflow INPUT_CHECK { - take: - samplesheet // file: /path/to/samplesheet.csv - - main: - SAMPLESHEET_CHECK ( samplesheet ) - .csv - .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channel(it) } - .set { reads } - - emit: - reads // channel: [ val(meta), [ reads ] ] - versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channel(LinkedHashMap row) { - // create meta map - def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() - - // add path(s) of the fastq file(s) to the meta map - def fastq_meta = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - fastq_meta = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" - } - fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return fastq_meta -} diff --git a/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf b/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf new file mode 100644 index 0000000..a0e1fe7 --- /dev/null +++ b/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf @@ -0,0 +1,212 @@ +// +// Subworkflow with functionality specific to the nf-core/pipeline pipeline +// + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { fromSamplesheet } from 'plugin/nf-validation' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' +include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' +include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' +include { nfCoreLogo } from '../../nf-core/utils_nfcore_pipeline' +include { imNotification } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' +include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' + +/* +======================================================================================== + SUBWORKFLOW TO INITIALISE PIPELINE +======================================================================================== +*/ + +workflow PIPELINE_INITIALISATION { + + take: + version // boolean: Display version and exit + help // boolean: Display help text + validate_params // boolean: Boolean whether to validate parameters against the schema at runtime + monochrome_logs // boolean: Do not use coloured log outputs + nextflow_cli_args // array: List of positional nextflow CLI args + outdir // string: The output directory where the results will be saved + input // string: Path to input samplesheet + + main: + + ch_versions = Channel.empty() + + // + // Print version and exit if required and dump pipeline parameters to JSON file + // + UTILS_NEXTFLOW_PIPELINE ( + version, + true, + outdir, + workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 + ) + + // + // Validate parameters and generate parameter summary to stdout + // + pre_help_text = nfCoreLogo(monochrome_logs) + post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) + def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input samplesheet.csv --outdir " + UTILS_NFVALIDATION_PLUGIN ( + help, + workflow_command, + pre_help_text, + post_help_text, + validate_params, + "nextflow_schema.json" + ) + + // + // Check config provided to the pipeline + // + UTILS_NFCORE_PIPELINE ( + nextflow_cli_args + ) + + // + // Create channel from input file provided through params.input + // + Channel + .fromSamplesheet("input") + .map { + meta, fastq_1, fastq_2 -> + if (!fastq_2) { + return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] + } else { + return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] + } + } + .groupTuple() + .map { + validateInputSamplesheet(it) + } + .map { + meta, fastqs -> + return [ meta, fastqs.flatten() ] + } + .set { ch_samplesheet } + + emit: + samplesheet = ch_samplesheet + versions = ch_versions +} + +/* +======================================================================================== + SUBWORKFLOW FOR PIPELINE COMPLETION +======================================================================================== +*/ + +workflow PIPELINE_COMPLETION { + + take: + email // string: email address + email_on_fail // string: email address sent on pipeline failure + plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published + monochrome_logs // boolean: Disable ANSI colour codes in log output + hook_url // string: hook URL for notifications + multiqc_report // string: Path to MultiQC report + + main: + + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + + // + // Completion email and summary + // + workflow.onComplete { + if (email || email_on_fail) { + completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + } + + completionSummary(monochrome_logs) + + if (hook_url) { + imNotification(summary_params, hook_url) + } + } +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/// +// Validate channels from input samplesheet +// +def validateInputSamplesheet(input) { + def (metas, fastqs) = input[1..2] + + // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end + def endedness_ok = metas.collect{ it.single_end }.unique().size == 1 + if (!endedness_ok) { + error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") + } + + return [ metas[0], fastqs ] +}// +// Generate methods description for MultiQC +// +def toolCitationText() { + // TODO nf-core: Optionally add in-text citation tools to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def citation_text = [ + "Tools used in the workflow included:", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text +} + +def toolBibliographyText() { + // TODO nf-core: Optionally add bibliographic entries to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def reference_text = [ + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text +} + +def methodsDescriptionText(mqc_methods_yaml) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = workflow.toMap() + meta["manifest_map"] = workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = "" + meta["tool_bibliography"] = "" + + // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! + // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + // meta["tool_bibliography"] = toolBibliographyText() + + + def methods_text = mqc_methods_yaml.text + + def engine = new groovy.text.SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html.toString() +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf new file mode 100644 index 0000000..ac31f28 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -0,0 +1,126 @@ +// +// Subworkflow with functionality that may be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NEXTFLOW_PIPELINE { + + take: + print_version // boolean: print version + dump_parameters // boolean: dump parameters + outdir // path: base directory used to publish pipeline results + check_conda_channels // boolean: check conda channels + + main: + + // + // Print workflow version and exit on --version + // + if (print_version) { + log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + System.exit(0) + } + + // + // Dump pipeline parameters to a JSON file + // + if (dump_parameters && outdir) { + dumpParametersToJSON(outdir) + } + + // + // When running with Conda, warn if channels have not been set-up appropriately + // + if (check_conda_channels) { + checkCondaChannels() + } + + emit: + dummy_emit = true +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Generate version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Dump pipeline parameters to a JSON file +// +def dumpParametersToJSON(outdir) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() +} + +// +// When running with -profile conda, warn if channels have not been set-up appropriately +// +def checkCondaChannels() { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml new file mode 100644 index 0000000..e5c3a0a --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml @@ -0,0 +1,38 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NEXTFLOW_PIPELINE" +description: Subworkflow with functionality that may be useful for any Nextflow pipeline +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - print_version: + type: boolean + description: | + Print the version of the pipeline and exit + - dump_parameters: + type: boolean + description: | + Dump the parameters of the pipeline to a JSON file + - output_directory: + type: directory + description: Path to output dir to write JSON file to. + pattern: "results/" + - check_conda_channel: + type: boolean + description: | + Check if the conda channel priority is correct. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" + - "@drpatelh" +maintainers: + - "@adamrtalbot" + - "@drpatelh" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test new file mode 100644 index 0000000..8ed4310 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -0,0 +1,54 @@ + +nextflow_function { + + name "Test Functions" + script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Test Function getWorkflowVersion") { + + function "getWorkflowVersion" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dumpParametersToJSON") { + + function "dumpParametersToJSON" + + when { + function { + """ + // define inputs of the function here. Example: + input[0] = "$outputDir" + """.stripIndent() + } + } + + then { + assertAll( + { assert function.success } + ) + } + } + + test("Test Function checkCondaChannels") { + + function "checkCondaChannels" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 0000000..db2030f --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,12 @@ +{ + "Test Function getWorkflowVersion": { + "content": [ + "v9.9.9" + ], + "timestamp": "2024-01-19T11:32:36.031083" + }, + "Test Function checkCondaChannels": { + "content": null, + "timestamp": "2024-01-19T11:32:50.456" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test new file mode 100644 index 0000000..f7c54bc --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,123 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NEXTFLOW_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + workflow "UTILS_NEXTFLOW_PIPELINE" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Should run no inputs") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = false + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should print version") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = true + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.stdout.contains("nextflow_workflow v9.9.9") } + ) + } + } + + test("Should dump params") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = params.outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should not create params JSON if no output directory") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = null + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config new file mode 100644 index 0000000..53574ff --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml new file mode 100644 index 0000000..f847611 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nextflow_pipeline: + - subworkflows/nf-core/utils_nextflow_pipeline/** diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf new file mode 100644 index 0000000..a8b55d6 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -0,0 +1,440 @@ +// +// Subworkflow with utility functions specific to the nf-core pipeline template +// + +import org.yaml.snakeyaml.Yaml +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFCORE_PIPELINE { + + take: + nextflow_cli_args + + main: + valid_config = checkConfigProvided() + checkProfileProvided(nextflow_cli_args) + + emit: + valid_config +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Warn if a -profile or Nextflow config has not been provided to run the pipeline +// +def checkConfigProvided() { + valid_config = true + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + valid_config = false + } + return valid_config +} + +// +// Exit pipeline if --profile contains spaces +// +def checkProfileProvided(nextflow_cli_args) { + if (workflow.profile.endsWith(',')) { + error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } + if (nextflow_cli_args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } +} + +// +// Citation string for pipeline +// +def workflowCitation() { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + " ${workflow.manifest.doi}\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" +} + +// +// Generate workflow version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Get software versions for pipeline +// +def processVersionsFromYAML(yaml_file) { + Yaml yaml = new Yaml() + versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + return yaml.dumpAsMap(versions).trim() +} + +// +// Get workflow version for pipeline +// +def workflowVersionToYAML() { + return """ + Workflow: + $workflow.manifest.name: ${getWorkflowVersion()} + Nextflow: $workflow.nextflow.version + """.stripIndent().trim() +} + +// +// Get channel of software versions used in pipeline in YAML format +// +def softwareVersionsToYAML(ch_versions) { + return ch_versions + .unique() + .map { processVersionsFromYAML(it) } + .unique() + .mix(Channel.of(workflowVersionToYAML())) +} + +// +// Get workflow summary for MultiQC +// +def paramsSummaryMultiqc(summary_params) { + def summary_section = '' + for (group in summary_params.keySet()) { + def group_params = summary_params.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + + return yaml_file_text +} + +// +// nf-core logo +// +def nfCoreLogo(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) +} + +// +// Return dashed line +// +def dashedLine(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" +} + +// +// ANSII colours used for terminal logging +// +def logColours(monochrome_logs=true) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes +} + +// +// Attach the multiqc report to email +// +def attachMultiqcReport(multiqc_report) { + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + return mqc_report +} + +// +// Construct and send completion email +// +def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = getWorkflowVersion() + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = attachMultiqcReport(multiqc_report) + + // Check if we are only sending emails on failure + def email_address = email + if (!email && email_on_fail && !workflow.success) { + email_address = email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("${workflow.projectDir}/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("${workflow.projectDir}/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(monochrome_logs) + if (email_address) { + try { + if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() +} + +// +// Print pipeline summary on completion +// +def completionSummary(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } +} + +// +// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack +// +def imNotification(summary_params, hook_url) { + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = getWorkflowVersion() + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("${workflow.projectDir}/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml new file mode 100644 index 0000000..d08d243 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml @@ -0,0 +1,24 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFCORE_PIPELINE" +description: Subworkflow with utility functions specific to the nf-core pipeline template +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - nextflow_cli_args: + type: list + description: | + Nextflow CLI positional arguments +output: + - success: + type: boolean + description: | + Dummy output to indicate success +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test new file mode 100644 index 0000000..1dc317f --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test @@ -0,0 +1,134 @@ + +nextflow_function { + + name "Test Functions" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Test Function checkConfigProvided") { + + function "checkConfigProvided" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function checkProfileProvided") { + + function "checkProfileProvided" + + when { + function { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function workflowCitation") { + + function "workflowCitation" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function nfCoreLogo") { + + function "nfCoreLogo" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dashedLine") { + + function "dashedLine" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function without logColours") { + + function "logColours" + + when { + function { + """ + input[0] = true + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function with logColours") { + function "logColours" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 0000000..10f948e --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,138 @@ +{ + "Test Function checkProfileProvided": { + "content": null, + "timestamp": "2024-02-09T15:43:55.145717" + }, + "Test Function checkConfigProvided": { + "content": [ + true + ], + "timestamp": "2024-01-19T11:34:13.548431224" + }, + "Test Function nfCoreLogo": { + "content": [ + "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" + ], + "timestamp": "2024-01-19T11:34:38.840454873" + }, + "Test Function workflowCitation": { + "content": [ + "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" + ], + "timestamp": "2024-01-19T11:34:22.24352016" + }, + "Test Function without logColours": { + "content": [ + { + "reset": "", + "bold": "", + "dim": "", + "underlined": "", + "blink": "", + "reverse": "", + "hidden": "", + "black": "", + "red": "", + "green": "", + "yellow": "", + "blue": "", + "purple": "", + "cyan": "", + "white": "", + "bblack": "", + "bred": "", + "bgreen": "", + "byellow": "", + "bblue": "", + "bpurple": "", + "bcyan": "", + "bwhite": "", + "ublack": "", + "ured": "", + "ugreen": "", + "uyellow": "", + "ublue": "", + "upurple": "", + "ucyan": "", + "uwhite": "", + "iblack": "", + "ired": "", + "igreen": "", + "iyellow": "", + "iblue": "", + "ipurple": "", + "icyan": "", + "iwhite": "", + "biblack": "", + "bired": "", + "bigreen": "", + "biyellow": "", + "biblue": "", + "bipurple": "", + "bicyan": "", + "biwhite": "" + } + ], + "timestamp": "2024-01-19T11:35:04.418416984" + }, + "Test Function dashedLine": { + "content": [ + "-\u001b[2m----------------------------------------------------\u001b[0m-" + ], + "timestamp": "2024-01-19T11:34:55.420000755" + }, + "Test Function with logColours": { + "content": [ + { + "reset": "\u001b[0m", + "bold": "\u001b[1m", + "dim": "\u001b[2m", + "underlined": "\u001b[4m", + "blink": "\u001b[5m", + "reverse": "\u001b[7m", + "hidden": "\u001b[8m", + "black": "\u001b[0;30m", + "red": "\u001b[0;31m", + "green": "\u001b[0;32m", + "yellow": "\u001b[0;33m", + "blue": "\u001b[0;34m", + "purple": "\u001b[0;35m", + "cyan": "\u001b[0;36m", + "white": "\u001b[0;37m", + "bblack": "\u001b[1;30m", + "bred": "\u001b[1;31m", + "bgreen": "\u001b[1;32m", + "byellow": "\u001b[1;33m", + "bblue": "\u001b[1;34m", + "bpurple": "\u001b[1;35m", + "bcyan": "\u001b[1;36m", + "bwhite": "\u001b[1;37m", + "ublack": "\u001b[4;30m", + "ured": "\u001b[4;31m", + "ugreen": "\u001b[4;32m", + "uyellow": "\u001b[4;33m", + "ublue": "\u001b[4;34m", + "upurple": "\u001b[4;35m", + "ucyan": "\u001b[4;36m", + "uwhite": "\u001b[4;37m", + "iblack": "\u001b[0;90m", + "ired": "\u001b[0;91m", + "igreen": "\u001b[0;92m", + "iyellow": "\u001b[0;93m", + "iblue": "\u001b[0;94m", + "ipurple": "\u001b[0;95m", + "icyan": "\u001b[0;96m", + "iwhite": "\u001b[0;97m", + "biblack": "\u001b[1;90m", + "bired": "\u001b[1;91m", + "bigreen": "\u001b[1;92m", + "biyellow": "\u001b[1;93m", + "biblue": "\u001b[1;94m", + "bipurple": "\u001b[1;95m", + "bicyan": "\u001b[1;96m", + "biwhite": "\u001b[1;97m" + } + ], + "timestamp": "2024-01-19T11:35:13.436366565" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test new file mode 100644 index 0000000..8940d32 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,29 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFCORE_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + workflow "UTILS_NFCORE_PIPELINE" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Should run without failures") { + + when { + workflow { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot(workflow.out).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap new file mode 100644 index 0000000..d07ce54 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -0,0 +1,15 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + true + ], + "valid_config": [ + true + ] + } + ], + "timestamp": "2024-01-19T11:35:22.538940073" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config new file mode 100644 index 0000000..d0a926b --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml new file mode 100644 index 0000000..ac8523c --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfcore_pipeline: + - subworkflows/nf-core/utils_nfcore_pipeline/** diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf new file mode 100644 index 0000000..2585b65 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf @@ -0,0 +1,62 @@ +// +// Subworkflow that uses the nf-validation plugin to render help text and parameter summary +// + +/* +======================================================================================== + IMPORT NF-VALIDATION PLUGIN +======================================================================================== +*/ + +include { paramsHelp } from 'plugin/nf-validation' +include { paramsSummaryLog } from 'plugin/nf-validation' +include { validateParameters } from 'plugin/nf-validation' + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFVALIDATION_PLUGIN { + + take: + print_help // boolean: print help + workflow_command // string: default commmand used to run pipeline + pre_help_text // string: string to be printed before help text and summary log + post_help_text // string: string to be printed after help text and summary log + validate_params // boolean: validate parameters + schema_filename // path: JSON schema file, null to use default value + + main: + + log.debug "Using schema file: ${schema_filename}" + + // Default values for strings + pre_help_text = pre_help_text ?: '' + post_help_text = post_help_text ?: '' + workflow_command = workflow_command ?: '' + + // + // Print help message if needed + // + if (print_help) { + log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text + System.exit(0) + } + + // + // Print parameter summary to stdout + // + log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text + + // + // Validate parameters relative to the parameter JSON schema + // + if (validate_params){ + validateParameters(parameters_schema: schema_filename) + } + + emit: + dummy_emit = true +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml new file mode 100644 index 0000000..3d4a6b0 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml @@ -0,0 +1,44 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFVALIDATION_PLUGIN" +description: Use nf-validation to initiate and validate a pipeline +keywords: + - utility + - pipeline + - initialise + - validation +components: [] +input: + - print_help: + type: boolean + description: | + Print help message and exit + - workflow_command: + type: string + description: | + The command to run the workflow e.g. "nextflow run main.nf" + - pre_help_text: + type: string + description: | + Text to print before the help message + - post_help_text: + type: string + description: | + Text to print after the help message + - validate_params: + type: boolean + description: | + Validate the parameters and error if invalid. + - schema_filename: + type: string + description: | + The filename of the schema to validate against. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test new file mode 100644 index 0000000..517ee54 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -0,0 +1,200 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFVALIDATION_PLUGIN" + script "../main.nf" + workflow "UTILS_NFVALIDATION_PLUGIN" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "plugin/nf-validation" + tag "'plugin/nf-validation'" + tag "utils_nfvalidation_plugin" + tag "subworkflows/utils_nfvalidation_plugin" + + test("Should run nothing") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should run help") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with command") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with extra text") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = "pre-help-text" + post_help_text = "post-help-text" + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('pre-help-text') } }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } }, + { assert workflow.stdout.any { it.contains('post-help-text') } } + ) + } + } + + test("Should validate params") { + + when { + + params { + monochrome_logs = true + test_data = '' + outdir = 1 + } + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = true + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json new file mode 100644 index 0000000..7626c1c --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json @@ -0,0 +1,96 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", + "title": ". pipeline parameters", + "description": "", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["outdir"], + "properties": { + "validate_params": { + "type": "boolean", + "description": "Validate parameters?", + "default": true, + "hidden": true + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "test_data_base": { + "type": "string", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules", + "description": "Base for test data directory", + "hidden": true + }, + "test_data": { + "type": "string", + "description": "Fake test data param", + "hidden": true + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "logo": { + "type": "boolean", + "default": true, + "description": "Display nf-core logo in console output.", + "fa_icon": "fas fa-image", + "hidden": true + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Pull Singularity container from Docker?", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Use monochrome_logs", + "hidden": true + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml new file mode 100644 index 0000000..60b1cff --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfvalidation_plugin: + - subworkflows/nf-core/utils_nfvalidation_plugin/** diff --git a/workflows/rangeland.nf b/workflows/rangeland.nf index 09192ea..d264d03 100644 --- a/workflows/rangeland.nf +++ b/workflows/rangeland.nf @@ -1,54 +1,15 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - PRINT PARAMS SUMMARY + IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' - -def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) -def citation = '\n' + WorkflowMain.citation(workflow) + '\n' -def summary_params = paramsSummaryMap(workflow) - -// Print parameter summary log to screen -log.info logo + paramsSummaryLog(workflow) + citation - -WorkflowRangeland.initialise(params, log) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CONFIG FILES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT LOCAL MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// -include { INPUT_CHECK } from '../subworkflows/local/input_check' - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT NF-CORE MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// MODULE: Installed directly from nf-core/modules -// -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' +include { FASTQC } from '../modules/nf-core/fastqc/main' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_rangeland_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -56,50 +17,45 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// Info required for completion email and summary -def multiqc_report = [] - workflow RANGELAND { - ch_versions = Channel.empty() + take: + ch_samplesheet // channel: samplesheet read in from --input - // - // SUBWORKFLOW: Read in samplesheet, validate and stage input files - // - INPUT_CHECK ( - file(params.input) - ) - ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) - // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") - // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ - // ! There is currently no tooling to help you write a sample sheet schema + main: + + ch_versions = Channel.empty() + ch_multiqc_files = Channel.empty() // // MODULE: Run FastQC // FASTQC ( - INPUT_CHECK.out.reads + ch_samplesheet ) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique().collectFile(name: 'collated_versions.yml') - ) + // + // Collate and save software versions + // + softwareVersionsToYAML(ch_versions) + .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) + .set { ch_collated_versions } // // MODULE: MultiQC // - workflow_summary = WorkflowRangeland.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - methods_description = WorkflowRangeland.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) - ch_methods_description = Channel.value(methods_description) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) MULTIQC ( ch_multiqc_files.collect(), @@ -107,31 +63,10 @@ workflow RANGELAND { ch_multiqc_custom_config.toList(), ch_multiqc_logo.toList() ) - multiqc_report = MULTIQC.out.report.toList() -} - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - COMPLETION EMAIL AND SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -workflow.onComplete { - if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) - } - NfcoreTemplate.dump_parameters(workflow, params) - NfcoreTemplate.summary(workflow, params, log) - if (params.hook_url) { - NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log) - } -} -workflow.onError { - if (workflow.errorReport.contains("Process requirement exceeds available memory")) { - println("🛑 Default resources exceed availability 🛑 ") - println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") - } + emit: + multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html + versions = ch_versions // channel: [ path(versions.yml) ] } /* From 9dc069b37988c862a361323f2be26b2a8572b98c Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Fri, 23 Feb 2024 09:34:35 +0100 Subject: [PATCH 07/61] Added version number to nf-validation plugin --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 37b8933..8a16a8b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -198,7 +198,7 @@ singularity.registry = 'quay.io' // Nextflow plugins plugins { - id 'nf-validation' // Validation of pipeline parameters and creation of an input channel from a sample sheet + id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet } // Export these variables to prevent local Python/R libraries from conflicting with those in the container From 4790c2d435e8e508c33d08fd9768e5ba013441cd Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 29 Feb 2024 16:11:41 +0000 Subject: [PATCH 08/61] Template update for nf-core/tools version 2.13.1 --- .devcontainer/devcontainer.json | 10 +---- .github/CONTRIBUTING.md | 14 ++++--- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/awsfulltest.yml | 4 +- .github/workflows/awstest.yml | 4 +- .github/workflows/ci.yml | 2 +- .github/workflows/download_pipeline.yml | 2 +- .github/workflows/linting.yml | 2 +- .github/workflows/release-announcements.yml | 2 +- .gitpod.yml | 6 +-- README.md | 3 +- modules.json | 8 ++-- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- .../nf-core/multiqc/tests/main.nf.test.snap | 12 +++--- .../utils_nfcore_rangeland_pipeline/main.nf | 10 +++-- .../tests/main.function.nf.test | 2 +- .../tests/main.function.nf.test.snap | 12 +++++- .../tests/main.workflow.nf.test | 20 ++------- .../tests/nextflow.config | 2 +- .../tests/main.function.nf.test.snap | 42 +++++++++++++++---- .../tests/main.workflow.nf.test.snap | 6 ++- .../tests/main.nf.test | 2 +- 23 files changed, 99 insertions(+), 74 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4ecfbfe..b290e09 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -10,15 +10,7 @@ "vscode": { // Set *default* container specific settings.json values on container create. "settings": { - "python.defaultInterpreterPath": "/opt/conda/bin/python", - "python.linting.enabled": true, - "python.linting.pylintEnabled": true, - "python.formatting.autopep8Path": "/opt/conda/bin/autopep8", - "python.formatting.yapfPath": "/opt/conda/bin/yapf", - "python.linting.flake8Path": "/opt/conda/bin/flake8", - "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", - "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" + "python.defaultInterpreterPath": "/opt/conda/bin/python" }, // Add the IDs of extensions you want installed when the container is created. diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index cde3aa6..18f544a 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,9 +9,8 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -:::info -If you need help using or modifying nf-core/rangeland then the best place to ask is on the nf-core Slack [#rangeland](https://nfcore.slack.com/channels/rangeland) channel ([join our Slack here](https://nf-co.re/join/slack)). -::: +> [!NOTE] +> If you need help using or modifying nf-core/rangeland then the best place to ask is on the nf-core Slack [#rangeland](https://nfcore.slack.com/channels/rangeland) channel ([join our Slack here](https://nf-co.re/join/slack)). ## Contribution workflow @@ -27,8 +26,11 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests -You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to -receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. +You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command: + +```bash +nf-test test --profile debug,test,docker --verbose +``` When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. @@ -90,7 +92,7 @@ Once there, use `nf-core schema build` to add to `nextflow_schema.json`. Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. -The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. +The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. ### Naming schemes diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 775ca31..77e4a6d 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rang - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rangeland/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/rangeland _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index fd38d18..567c485 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 + uses: seqeralabs/action-tower-launch@v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 23daa39..ecdfecd 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,7 +12,7 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 + uses: seqeralabs/action-tower-launch@v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 5b8d0d1..3a8eb8e 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -31,7 +31,7 @@ jobs: uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 with: version: "${{ matrix.NXF_VER }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index f823210..08622fd 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -28,7 +28,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 748b431..073e187 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -35,7 +35,7 @@ jobs: uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index c3674af..d468aea 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -12,7 +12,7 @@ jobs: - name: get topics and convert to hashtags id: get_topics run: | - curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' > $GITHUB_OUTPUT + curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT - uses: rzr/fediverse-action@master with: diff --git a/.gitpod.yml b/.gitpod.yml index 363d5b1..105a182 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -10,13 +10,11 @@ tasks: vscode: extensions: # based on nf-core.nf-core-extensionpack - - codezombiech.gitignore # Language support for .gitignore files - # - cssho.vscode-svgviewer # SVG viewer - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code - - eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar - mechatroner.rainbow-csv # Highlight columns in csv files in different colors - # - nextflow.nextflow # Nextflow syntax highlighting + # - nextflow.nextflow # Nextflow syntax highlighting - oderwat.indent-rainbow # Highlight indentation level - streetsidesoftware.code-spell-checker # Spelling checker for source code + - charliermarsh.ruff # Code linter Ruff diff --git a/README.md b/README.md index c925d65..f658f3f 100644 --- a/README.md +++ b/README.md @@ -7,12 +7,13 @@ [![GitHub Actions CI Status](https://github.com/nf-core/rangeland/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rangeland/actions/workflows/ci.yml) [![GitHub Actions Linting Status](https://github.com/nf-core/rangeland/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rangeland/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rangeland/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/rangeland) +[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/rangeland) [![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rangeland-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rangeland)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) diff --git a/modules.json b/modules.json index 68efc57..0694f9f 100644 --- a/modules.json +++ b/modules.json @@ -12,7 +12,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290", + "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", "installed_by": ["modules"] } } @@ -21,17 +21,17 @@ "nf-core": { "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] } } diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 2212096..ca39fb6 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.20 + - bioconda::multiqc=1.21 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 354f443..47ac352 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : - 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : + 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index c204b48..bfebd80 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -2,14 +2,14 @@ "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:28:51.744211298" + "timestamp": "2024-02-29T08:48:55.657331" }, "multiqc_stub": { "content": [ @@ -17,25 +17,25 @@ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:29:28.847433492" + "timestamp": "2024-02-29T08:49:49.071937" }, "multiqc_versions_config": { "content": [ [ - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:29:13.223621555" + "timestamp": "2024-02-29T08:49:25.457567" } } \ No newline at end of file diff --git a/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf b/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf index a0e1fe7..35a0e43 100644 --- a/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf @@ -1,5 +1,5 @@ // -// Subworkflow with functionality specific to the nf-core/pipeline pipeline +// Subworkflow with functionality specific to the nf-core/rangeland pipeline // /* @@ -142,7 +142,9 @@ workflow PIPELINE_COMPLETION { ======================================================================================== FUNCTIONS ======================================================================================== -*/// +*/ + +// // Validate channels from input samplesheet // def validateInputSamplesheet(input) { @@ -155,7 +157,9 @@ def validateInputSamplesheet(input) { } return [ metas[0], fastqs ] -}// +} + +// // Generate methods description for MultiQC // def toolCitationText() { diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test index 8ed4310..68718e4 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -51,4 +51,4 @@ nextflow_function { ) } } -} \ No newline at end of file +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap index db2030f..e3f0baf 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -3,10 +3,18 @@ "content": [ "v9.9.9" ], - "timestamp": "2024-01-19T11:32:36.031083" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:05.308243" }, "Test Function checkCondaChannels": { "content": null, - "timestamp": "2024-01-19T11:32:50.456" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:12.425833" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test index f7c54bc..ca964ce 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -11,9 +11,6 @@ nextflow_workflow { test("Should run no inputs") { when { - params { - outdir = "tests/results" - } workflow { """ print_version = false @@ -39,9 +36,6 @@ nextflow_workflow { test("Should print version") { when { - params { - outdir = "tests/results" - } workflow { """ print_version = true @@ -68,19 +62,16 @@ nextflow_workflow { test("Should dump params") { when { - params { - outdir = "$outputDir" - } workflow { """ print_version = false dump_parameters = true - outdir = params.outdir + outdir = 'results' check_conda_channels = false input[0] = false input[1] = true - input[2] = params.outdir + input[2] = outdir input[3] = false """ } @@ -96,19 +87,16 @@ nextflow_workflow { test("Should not create params JSON if no output directory") { when { - params { - outdir = "$outputDir" - } workflow { """ print_version = false dump_parameters = true - outdir = params.outdir + outdir = null check_conda_channels = false input[0] = false input[1] = true - input[2] = null + input[2] = outdir input[3] = false """ } diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config index 53574ff..d0a926b 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -6,4 +6,4 @@ manifest { nextflowVersion = '!>=23.04.0' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' -} \ No newline at end of file +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap index 10f948e..1037232 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -1,25 +1,41 @@ { "Test Function checkProfileProvided": { "content": null, - "timestamp": "2024-02-09T15:43:55.145717" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:03.360873" }, "Test Function checkConfigProvided": { "content": [ true ], - "timestamp": "2024-01-19T11:34:13.548431224" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:59.729647" }, "Test Function nfCoreLogo": { "content": [ "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" ], - "timestamp": "2024-01-19T11:34:38.840454873" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:10.562934" }, "Test Function workflowCitation": { "content": [ "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" ], - "timestamp": "2024-01-19T11:34:22.24352016" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:07.019761" }, "Test Function without logColours": { "content": [ @@ -73,13 +89,21 @@ "biwhite": "" } ], - "timestamp": "2024-01-19T11:35:04.418416984" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:17.969323" }, "Test Function dashedLine": { "content": [ "-\u001b[2m----------------------------------------------------\u001b[0m-" ], - "timestamp": "2024-01-19T11:34:55.420000755" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:14.366181" }, "Test Function with logColours": { "content": [ @@ -133,6 +157,10 @@ "biwhite": "\u001b[1;97m" } ], - "timestamp": "2024-01-19T11:35:13.436366565" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:21.714424" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap index d07ce54..859d103 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -10,6 +10,10 @@ ] } ], - "timestamp": "2024-01-19T11:35:22.538940073" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:25.726491" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test index 517ee54..5784a33 100644 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -197,4 +197,4 @@ nextflow_workflow { ) } } -} \ No newline at end of file +} From dd9e3da35c2dd7b1629a977e24106a78540d00d5 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Wed, 8 May 2024 14:01:16 +0000 Subject: [PATCH 09/61] Template update for nf-core/tools version 2.14.0 --- .editorconfig | 6 +- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/awsfulltest.yml | 10 +- .github/workflows/awstest.yml | 12 +- .github/workflows/ci.yml | 4 +- .github/workflows/download_pipeline.yml | 22 ++- .github/workflows/fix-linting.yml | 6 +- .github/workflows/linting.yml | 18 +- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 6 +- .nf-core.yml | 1 + .pre-commit-config.yaml | 3 + README.md | 2 +- conf/base.config | 3 - conf/modules.config | 8 - conf/test.config | 2 +- conf/test_full.config | 4 +- docs/usage.md | 2 + modules.json | 4 +- modules/nf-core/fastqc/main.nf | 6 + nextflow.config | 177 +++++++++--------- nextflow_schema.json | 7 + pyproject.toml | 15 -- .../utils_nfcore_rangeland_pipeline/main.nf | 16 +- .../nf-core/utils_nfcore_pipeline/main.nf | 8 +- workflows/rangeland.nf | 46 +++-- 26 files changed, 222 insertions(+), 170 deletions(-) delete mode 100644 pyproject.toml diff --git a/.editorconfig b/.editorconfig index dd9ffa5..72dda28 100644 --- a/.editorconfig +++ b/.editorconfig @@ -28,10 +28,6 @@ indent_style = unset [/assets/email*] indent_size = unset -# ignore Readme -[README.md] -indent_style = unset - -# ignore python +# ignore python and markdown [*.{py,md}] indent_style = unset diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 77e4a6d..775ca31 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rang - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rangeland/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/rangeland _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). +- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 567c485..c16cc45 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -8,12 +8,12 @@ on: types: [published] workflow_dispatch: jobs: - run-tower: + run-platform: name: Run AWS full tests if: github.repository == 'nf-core/rangeland' runs-on: ubuntu-latest steps: - - name: Launch workflow via tower + - name: Launch workflow via Seqera Platform uses: seqeralabs/action-tower-launch@v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) @@ -33,7 +33,7 @@ jobs: - uses: actions/upload-artifact@v4 with: - name: Tower debug log file + name: Seqera Platform debug log file path: | - tower_action_*.log - tower_action_*.json + seqera_platform_action_*.log + seqera_platform_action_*.json diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index ecdfecd..250e14b 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -5,13 +5,13 @@ name: nf-core AWS test on: workflow_dispatch: jobs: - run-tower: + run-platform: name: Run AWS tests if: github.repository == 'nf-core/rangeland' runs-on: ubuntu-latest steps: - # Launch workflow using Tower CLI tool action - - name: Launch workflow via tower + # Launch workflow using Seqera Platform CLI tool action + - name: Launch workflow via Seqera Platform uses: seqeralabs/action-tower-launch@v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} @@ -27,7 +27,7 @@ jobs: - uses: actions/upload-artifact@v4 with: - name: Tower debug log file + name: Seqera Platform debug log file path: | - tower_action_*.log - tower_action_*.json + seqera_platform_action_*.log + seqera_platform_action_*.json diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 3a8eb8e..7e6f66a 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,10 +28,10 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 with: version: "${{ matrix.NXF_VER }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 08622fd..2d20d64 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -14,6 +14,8 @@ on: pull_request: types: - opened + - edited + - synchronize branches: - master pull_request_target: @@ -28,11 +30,14 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: "3.11" + python-version: "3.12" architecture: "x64" - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 with: @@ -65,8 +70,17 @@ jobs: - name: Inspect download run: tree ./${{ env.REPOTITLE_LOWERCASE }} - - name: Run the downloaded pipeline + - name: Run the downloaded pipeline (stub) + id: stub_run_pipeline + continue-on-error: true env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + - name: Run the downloaded pipeline (stub run not supported) + id: run_pipeline + if: ${{ job.steps.stub_run_pipeline.status == failure() }} + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 06cc19a..da19b1c 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -13,7 +13,7 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 with: token: ${{ secrets.nf_core_bot_auth_token }} @@ -32,9 +32,9 @@ jobs: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} # Install and run pre-commit - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: 3.11 + python-version: "3.12" - name: Install pre-commit run: pip install pre-commit diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 073e187..a3fb254 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,12 +14,12 @@ jobs: pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - - name: Set up Python 3.11 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - name: Set up Python 3.12 + uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: 3.11 + python-version: "3.12" cache: "pip" - name: Install pre-commit @@ -32,14 +32,14 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: "3.11" + python-version: "3.12" architecture: "x64" - name: Install dependencies @@ -60,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + uses: actions/upload-artifact@65462800fd760344b1a7b4382951275a0abb4808 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index b706875..40acc23 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 + uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index d468aea..03ecfcf 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -12,7 +12,7 @@ jobs: - name: get topics and convert to hashtags id: get_topics run: | - curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT + echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')" >> $GITHUB_OUTPUT - uses: rzr/fediverse-action@master with: @@ -25,13 +25,13 @@ jobs: Please see the changelog: ${{ github.event.release.html_url }} - ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics + ${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics send-tweet: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: python-version: "3.10" - name: Install dependencies diff --git a/.nf-core.yml b/.nf-core.yml index af643f6..1bb0832 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,3 +1,4 @@ +nf_core_version: 2.14.0 repository_type: pipeline template: prefix: nf-core diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index af57081..4dc0f1d 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -3,6 +3,9 @@ repos: rev: "v3.1.0" hooks: - id: prettier + additional_dependencies: + - prettier@3.2.5 + - repo: https://github.com/editorconfig-checker/editorconfig-checker.python rev: "2.7.3" hooks: diff --git a/README.md b/README.md index f658f3f..2e9df7f 100644 --- a/README.md +++ b/README.md @@ -13,7 +13,7 @@ [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/rangeland) +[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rangeland) [![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rangeland-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rangeland)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) diff --git a/conf/base.config b/conf/base.config index ab8916b..76e4569 100644 --- a/conf/base.config +++ b/conf/base.config @@ -59,7 +59,4 @@ process { errorStrategy = 'retry' maxRetries = 2 } - withName:CUSTOM_DUMPSOFTWAREVERSIONS { - cache = false - } } diff --git a/conf/modules.config b/conf/modules.config index e3ea8fa..d203d2b 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -22,14 +22,6 @@ process { ext.args = '--quiet' } - withName: CUSTOM_DUMPSOFTWAREVERSIONS { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - pattern: '*_versions.yml' - ] - } - withName: 'MULTIQC' { ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ diff --git a/conf/test.config b/conf/test.config index 0a3709b..2e8bff0 100644 --- a/conf/test.config +++ b/conf/test.config @@ -22,7 +22,7 @@ params { // Input data // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' + input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' } diff --git a/conf/test_full.config b/conf/test_full.config index 21fc4b4..024a55c 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -17,8 +17,8 @@ params { // Input data for full size test // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' + input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' // Fasta references - fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/genome/NC_045512.2/GCF_009858895.2_ASM985889v3_genomic.200409.fna.gz' + fasta = params.pipelines_testdata_base_path + 'viralrecon/genome/NC_045512.2/GCF_009858895.2_ASM985889v3_genomic.200409.fna.gz' } diff --git a/docs/usage.md b/docs/usage.md index a4ed357..bd88671 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -156,6 +156,8 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) - `apptainer` - A generic configuration profile to be used with [Apptainer](https://apptainer.org/) +- `wave` + - A generic configuration profile to enable [Wave](https://seqera.io/wave/) containers. Use together with one of the above (requires Nextflow ` 24.03.0-edge` or later). - `conda` - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer. diff --git a/modules.json b/modules.json index 0694f9f..22f15e0 100644 --- a/modules.json +++ b/modules.json @@ -7,7 +7,7 @@ "nf-core": { "fastqc": { "branch": "master", - "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", + "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", "installed_by": ["modules"] }, "multiqc": { @@ -26,7 +26,7 @@ }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 9e19a74..d79f1c8 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -25,6 +25,11 @@ process FASTQC { def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } def rename_to = old_new_pairs*.join(' ').join(' ') def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') + + def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') + // FastQC memory value allowed range (100 - 10000) + def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb) + """ printf "%s %s\\n" $rename_to | while read old_name new_name; do [ -f "\${new_name}" ] || ln -s \$old_name \$new_name @@ -33,6 +38,7 @@ process FASTQC { fastqc \\ $args \\ --threads $task.cpus \\ + --memory $fastqc_memory \\ $renamed_files cat <<-END_VERSIONS > versions.yml diff --git a/nextflow.config b/nextflow.config index aac9a2b..94ab6c8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -11,7 +11,9 @@ params { // TODO nf-core: Specify your pipeline's command line flags // Input options - input = null// MultiQC options + input = null + + // MultiQC options multiqc_config = null multiqc_title = null multiqc_logo = null @@ -19,15 +21,16 @@ params { multiqc_methods_description = null // Boilerplate options - outdir = null - publish_dir_mode = 'copy' - email = null - email_on_fail = null - plaintext_email = false - monochrome_logs = false - hook_url = null - help = false - version = false + outdir = null + publish_dir_mode = 'copy' + email = null + email_on_fail = null + plaintext_email = false + monochrome_logs = false + hook_url = null + help = false + version = false + pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/' // Config options config_profile_name = null @@ -63,103 +66,109 @@ try { } // Load nf-core/rangeland custom profiles from different institutions. -// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs! -// try { -// includeConfig "${params.custom_config_base}/pipeline/rangeland.config" -// } catch (Exception e) { -// System.err.println("WARNING: Could not load nf-core/config/rangeland profiles: ${params.custom_config_base}/pipeline/rangeland.config") -// } +try { + includeConfig "${params.custom_config_base}/pipeline/rangeland.config" +} catch (Exception e) { + System.err.println("WARNING: Could not load nf-core/config/rangeland profiles: ${params.custom_config_base}/pipeline/rangeland.config") +} profiles { debug { - dumpHashes = true - process.beforeScript = 'echo $HOSTNAME' - cleanup = false + dumpHashes = true + process.beforeScript = 'echo $HOSTNAME' + cleanup = false nextflow.enable.configProcessNamesValidation = true } conda { - conda.enabled = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - channels = ['conda-forge', 'bioconda', 'defaults'] - apptainer.enabled = false + conda.enabled = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + conda.channels = ['conda-forge', 'bioconda', 'defaults'] + apptainer.enabled = false } mamba { - conda.enabled = true - conda.useMamba = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false + conda.enabled = true + conda.useMamba = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } docker { - docker.enabled = true - conda.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false - docker.runOptions = '-u $(id -u):$(id -g)' + docker.enabled = true + conda.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false + docker.runOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { - singularity.enabled = true - singularity.autoMounts = true - conda.enabled = false - docker.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false + singularity.enabled = true + singularity.autoMounts = true + conda.enabled = false + docker.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } podman { - podman.enabled = true - conda.enabled = false - docker.enabled = false - singularity.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false + podman.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } shifter { - shifter.enabled = true - conda.enabled = false - docker.enabled = false - singularity.enabled = false - podman.enabled = false - charliecloud.enabled = false - apptainer.enabled = false + shifter.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } charliecloud { - charliecloud.enabled = true - conda.enabled = false - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - apptainer.enabled = false + charliecloud.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + apptainer.enabled = false } apptainer { - apptainer.enabled = true - apptainer.autoMounts = true - conda.enabled = false - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false + apptainer.enabled = true + apptainer.autoMounts = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + wave { + apptainer.ociAutoPull = true + singularity.ociAutoPull = true + wave.enabled = true + wave.freeze = true + wave.strategy = 'conda,container' } gitpod { - executor.name = 'local' - executor.cpus = 4 - executor.memory = 8.GB + executor.name = 'local' + executor.cpus = 4 + executor.memory = 8.GB } test { includeConfig 'conf/test.config' } test_full { includeConfig 'conf/test_full.config' } diff --git a/nextflow_schema.json b/nextflow_schema.json index 9d71dd1..0dc3e47 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -234,6 +234,13 @@ "description": "Validation of parameters in lenient more.", "hidden": true, "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." + }, + "pipelines_testdata_base_path": { + "type": "string", + "fa_icon": "far fa-check-circle", + "description": "Base URL or local path to location of pipeline test dataset files", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/", + "hidden": true } } } diff --git a/pyproject.toml b/pyproject.toml deleted file mode 100644 index 5611062..0000000 --- a/pyproject.toml +++ /dev/null @@ -1,15 +0,0 @@ -# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. -# Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.ruff] -line-length = 120 -target-version = "py38" -cache-dir = "~/.cache/ruff" - -[tool.ruff.lint] -select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] - -[tool.ruff.lint.isort] -known-first-party = ["nf_core"] - -[tool.ruff.lint.per-file-ignores] -"__init__.py" = ["E402", "F401"] diff --git a/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf b/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf index 35a0e43..4dcd0fe 100644 --- a/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf @@ -136,6 +136,10 @@ workflow PIPELINE_COMPLETION { imNotification(summary_params, hook_url) } } + + workflow.onError { + log.error "Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting" + } } /* @@ -195,8 +199,16 @@ def methodsDescriptionText(mqc_methods_yaml) { meta["manifest_map"] = workflow.manifest.toMap() // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + if (meta.manifest_map.doi) { + // Using a loop to handle multiple DOIs + // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers + // Removing ` ` since the manifest.doi is a string and not a proper list + def temp_doi_ref = "" + String[] manifest_doi = meta.manifest_map.doi.tokenize(",") + for (String doi_ref: manifest_doi) temp_doi_ref += "(doi: ${doi_ref.replace("https://doi.org/", "").replace(" ", "")}), " + meta["doi_text"] = temp_doi_ref.substring(0, temp_doi_ref.length() - 2) + } else meta["doi_text"] = "" + meta["nodoi_text"] = meta.manifest_map.doi ? "" : "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " // Tool references meta["tool_citations"] = "" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf index a8b55d6..14558c3 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -65,9 +65,15 @@ def checkProfileProvided(nextflow_cli_args) { // Citation string for pipeline // def workflowCitation() { + def temp_doi_ref = "" + String[] manifest_doi = workflow.manifest.doi.tokenize(",") + // Using a loop to handle multiple DOIs + // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers + // Removing ` ` since the manifest.doi is a string and not a proper list + for (String doi_ref: manifest_doi) temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + "* The pipeline\n" + - " ${workflow.manifest.doi}\n\n" + + temp_doi_ref + "\n" + "* The nf-core framework\n" + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + diff --git a/workflows/rangeland.nf b/workflows/rangeland.nf index d264d03..568c5a7 100644 --- a/workflows/rangeland.nf +++ b/workflows/rangeland.nf @@ -40,22 +40,44 @@ workflow RANGELAND { // Collate and save software versions // softwareVersionsToYAML(ch_versions) - .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) - .set { ch_collated_versions } + .collectFile( + storeDir: "${params.outdir}/pipeline_info", + name: 'nf_core_pipeline_software_mqc_versions.yml', + sort: true, + newLine: true + ).set { ch_collated_versions } // // MODULE: MultiQC // - ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) - ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() - ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() - summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") - ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) - ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) + ch_multiqc_config = Channel.fromPath( + "$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? + Channel.fromPath(params.multiqc_config, checkIfExists: true) : + Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? + Channel.fromPath(params.multiqc_logo, checkIfExists: true) : + Channel.empty() + + summary_params = paramsSummaryMap( + workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? + file(params.multiqc_methods_description, checkIfExists: true) : + file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value( + methodsDescriptionText(ch_multiqc_custom_methods_description)) + + ch_multiqc_files = ch_multiqc_files.mix( + ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix( + ch_methods_description.collectFile( + name: 'methods_description_mqc.yaml', + sort: true + ) + ) MULTIQC ( ch_multiqc_files.collect(), From d6e1398efe877a92382fbd382cf8b2aad48ae7c8 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 9 May 2024 11:42:59 +0000 Subject: [PATCH 10/61] Template update for nf-core/tools version 2.14.1 --- .github/workflows/linting.yml | 1 - .nf-core.yml | 2 +- 2 files changed, 1 insertion(+), 2 deletions(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index a3fb254..1fcafe8 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -20,7 +20,6 @@ jobs: uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: python-version: "3.12" - cache: "pip" - name: Install pre-commit run: pip install pre-commit diff --git a/.nf-core.yml b/.nf-core.yml index 1bb0832..d9b4c86 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,4 +1,4 @@ -nf_core_version: 2.14.0 +nf_core_version: 2.14.1 repository_type: pipeline template: prefix: nf-core From 841399d2c65663e3bd80f39dc7626fd7177eea75 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Fri, 10 May 2024 16:00:29 +0200 Subject: [PATCH 11/61] Removed unnecessary resource requests in modules.conf in favor of nf-core labels --- conf/modules.config | 25 ------------------------- modules/local/force-higher_level.nf | 2 +- 2 files changed, 1 insertion(+), 26 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 9f8a3df..12bc048 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -35,8 +35,6 @@ process { } withName: "FORCE_GENERATE_ANALYSIS_MASK" { - memory = { 500.MB * task.attempt } - time = { 20.minute * task.attempt } publishDir = [ path: { "${params.outdir}/preparation/" }, mode: params.publish_dir_mode, @@ -45,8 +43,6 @@ process { } withName: "FORCE_GENERATE_TILE_ALLOW_LIST" { - memory = { 500.MB * task.attempt } - time = { 20.minute * task.attempt } publishDir = [ path: { "${params.outdir}/preparation/" }, pattern: '*.txt', @@ -55,9 +51,6 @@ process { } withName: "FORCE_PREPROCESS" { - cpus = 4 - memory = { 4500.MB * task.attempt } - time = { 2.h * task.attempt } errorStrategy = 'retry' maxRetries = 5 publishDir = [ @@ -74,15 +67,6 @@ process { ] } - withName: "MERGE_BOA" { - memory = { 2000.MB * task.attempt } - time = { 2.h * task.attempt } - } - - withName: "MERGE_QAI" { - memory = { 2000.MB * task.attempt } - time = { 2.h * task.attempt } - } withName: "HIGHER_LEVEL_CONFIG" { errorStrategy = 'retry' @@ -95,9 +79,6 @@ process { } withName: "FORCE_HIGHER_LEVEL" { - cpus = 6 - memory = { (params.only_tile ? 12000.MB : 3300.MB ) * task.attempt } - time = { 2.h * task.attempt } publishDir = [ path: { "${params.outdir}/higher-level/${task.tag}" }, mode: 'symlink', @@ -107,8 +88,6 @@ process { } withName: "FORCE_PYRAMID" { - memory = { 1500.MB * task.attempt } - time = { 20.minute * task.attempt } stageInMode = 'copy' publishDir = [ [ @@ -126,8 +105,6 @@ process { } withName: "FORCE_MOSAIC" { - memory = { 1500.MB * task.attempt } - time = { 20.minute * task.attempt } publishDir = [ path: { "${params.outdir}/trend/mosaic/" }, mode: params.publish_dir_mode, @@ -137,8 +114,6 @@ process { withName: "CHECK_RESULTS" { errorStrategy = { task.exitStatus == 143 ? 'retry' : 'ignore' } - memory = { 14000.MB * task.attempt } - time = { 2.h * task.attempt } publishDir = [ enabled: false ] diff --git a/modules/local/force-higher_level.nf b/modules/local/force-higher_level.nf index dfd8d80..c91d9b4 100644 --- a/modules/local/force-higher_level.nf +++ b/modules/local/force-higher_level.nf @@ -2,7 +2,7 @@ nextflow.enable.dsl = 2 process FORCE_HIGHER_LEVEL { - label 'process_high' + label 'process_medium' container "docker.io/davidfrantz/force:3.7.10" tag { tile } From 8c1c9d9f73a00894c875aae7c2c641ec9035cf9b Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Thu, 16 May 2024 12:26:48 +0200 Subject: [PATCH 12/61] fixed docker test failures in certain situations (e.g. nf-core github actions) --- nextflow.config | 2 ++ 1 file changed, 2 insertions(+) diff --git a/nextflow.config b/nextflow.config index 8a16a8b..558fa9c 100644 --- a/nextflow.config +++ b/nextflow.config @@ -128,6 +128,8 @@ profiles { shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false + docker.fixOwnership = true + docker.runOptions = '-u $(id -u):$(id -g)' } arm { docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' From 6fb5757f38359500a4e23ac50017ae06d0004e4b Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Fri, 17 May 2024 11:44:49 +0200 Subject: [PATCH 13/61] Ensured that remaining tests run after a single test failure --- bin/test.R | 37 ++++++++++++++++++++++++------------- 1 file changed, 24 insertions(+), 13 deletions(-) diff --git a/bin/test.R b/bin/test.R index 7274a98..fbaa9a1 100755 --- a/bin/test.R +++ b/bin/test.R @@ -83,50 +83,61 @@ peak_year_of_change <- peak_rast["YEAR-OF-CHANGE"] # COMPARE TESTRUN WITH REFERENCE EXECUTION ####################################################################### +failure <- FALSE woody_cover_changes_result <- all.equal(woody_cover_changes, woody_cover_changes_ref) -if (is.character(woody_cover_changes_result)){ - stop("Error: ", paste0(woody_cover_changes_result, " for woody cover changes.")) +if (is.character(woody_cover_changes_result)) { + print(paste0("Error: ", woody_cover_changes_result, " for woody cover changes.")) + failure <- TRUE } else { print("Woody cover change check passed.") } woody_cover_year_of_change_result <- all.equal(woody_cover_year_of_change, woody_cover_year_of_change_ref) -if (is.character(woody_cover_year_of_change_result)){ - stop("Error: ", paste0(woody_cover_year_of_change_result, " for woody cover year of change.")) +if (is.character(woody_cover_year_of_change_result)) { + print(paste0("Error: ", woody_cover_year_of_change_result, " for woody cover year of change.")) + failure <- TRUE } else { print("Woody cover year of change check passed.") } herbaceous_cover_changes_result <- all.equal(herbaceous_cover_changes, herbaceous_cover_changes_ref) -if (is.character(herbaceous_cover_changes_result)){ - stop("Error: ", paste0(herbaceous_cover_changes_result, " for herbaceous cover changes.")) +if (is.character(herbaceous_cover_changes_result)) { + print(paste0("Error: ",herbaceous_cover_changes_result, " for herbaceous cover changes.")) + failure <- TRUE } else { print("Herbaceous cover change check passed.") } herbaceous_cover_year_of_change_result <- all.equal(herbaceous_cover_year_of_change, herbaceous_cover_year_of_change_ref) -if (is.character(herbaceous_cover_year_of_change_result)){ - stop("Error: ", paste0(herbaceous_cover_year_of_change_result, " for herbaceous cover year of change.")) +if (is.character(herbaceous_cover_year_of_change_result)) { + print(paste0("Error: ", herbaceous_cover_year_of_change_result, " for herbaceous cover year of change.")) + failure <- TRUE } else { print("Herbaceous cover year of change check passed.") } peak_changes_result <- all.equal(peak_changes, peak_changes_ref) -if (is.character(peak_changes_result)){ - stop("Error: ", paste0(peak_changes_result, " for peak changes.")) +if (is.character(peak_changes_result)) { + print(paste0("Error: ", peak_changes_result, " for peak changes.")) + failure <- TRUE } else { print("Peak change check passed.") } peak_year_of_change_result <- all.equal(peak_year_of_change, peak_year_of_change_ref) -if (is.character(peak_year_of_change_result)){ - stop("Error: ", paste0(peak_year_of_change_result, " for peak year of change.")) +if (is.character(peak_year_of_change_result)) { + print(paste0("Error: ", peak_year_of_change_result, " for peak year of change.")) + failure <- TRUE } else { print("Peak year of change check passed.") } -print("All checks passed.") +if (failure) { + stop("Some test failed.") +} else { + print("All checks passed.") +} From 9b17ae70d0387cf37511c7c33999e58f7ca5b5dc Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Fri, 24 May 2024 15:52:57 +0200 Subject: [PATCH 14/61] Fixed indents in merge scripts --- bin/merge_boa.r | 2 +- bin/merge_qai.r | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/bin/merge_boa.r b/bin/merge_boa.r index 98a0afd..27779ad 100755 --- a/bin/merge_boa.r +++ b/bin/merge_boa.r @@ -4,7 +4,7 @@ args = commandArgs(trailingOnly=TRUE) if (length(args) < 3) { - stop("\nthis program needs at least 3 inputs\n1: output filename\n2-*: input files", call.=FALSE) + stop("\nthis program needs at least 3 inputs\n1: output filename\n2-*: input files", call.=FALSE) } fout <- args[1] diff --git a/bin/merge_qai.r b/bin/merge_qai.r index 9a31421..fc9066f 100755 --- a/bin/merge_qai.r +++ b/bin/merge_qai.r @@ -4,7 +4,7 @@ args = commandArgs(trailingOnly=TRUE) if (length(args) < 3) { - stop("\nthis program needs at least 3 inputs\n1: output filename\n2-*: input files", call.=FALSE) + stop("\nthis program needs at least 3 inputs\n1: output filename\n2-*: input files", call.=FALSE) } fout <- args[1] From 665e8624760b0c0e6e81692311fa7c43939d3d77 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Thu, 30 May 2024 10:17:42 +0200 Subject: [PATCH 15/61] Added appropriate raster comparisons for trend tif's to test.R --- bin/test.R | 18 +++++++++++++++--- 1 file changed, 15 insertions(+), 3 deletions(-) diff --git a/bin/test.R b/bin/test.R index fbaa9a1..7c88bbb 100755 --- a/bin/test.R +++ b/bin/test.R @@ -15,6 +15,18 @@ dinp <- args[1] # load package require(terra) +# function to compare raster values with tolerance +compare_rasters <- function(r1, r2, tolerance = 1e-5) { + + diff <- abs(values(r1) - values(r2)) + max_diff <- max(diff, na.rm = TRUE) + + if (max_diff > tolerance) { + return(paste("Max difference", max_diff, "exceeds tolerance", tolerance)) + } + + return(TRUE) +} # LOAD REFERENCE @@ -85,7 +97,7 @@ peak_year_of_change <- peak_rast["YEAR-OF-CHANGE"] ####################################################################### failure <- FALSE -woody_cover_changes_result <- all.equal(woody_cover_changes, woody_cover_changes_ref) +woody_cover_changes_result <- compare_rasters(woody_cover_changes, woody_cover_changes_ref) if (is.character(woody_cover_changes_result)) { print(paste0("Error: ", woody_cover_changes_result, " for woody cover changes.")) failure <- TRUE @@ -102,7 +114,7 @@ if (is.character(woody_cover_year_of_change_result)) { } -herbaceous_cover_changes_result <- all.equal(herbaceous_cover_changes, herbaceous_cover_changes_ref) +herbaceous_cover_changes_result <- compare_rasters(herbaceous_cover_changes, herbaceous_cover_changes_ref) if (is.character(herbaceous_cover_changes_result)) { print(paste0("Error: ",herbaceous_cover_changes_result, " for herbaceous cover changes.")) failure <- TRUE @@ -119,7 +131,7 @@ if (is.character(herbaceous_cover_year_of_change_result)) { } -peak_changes_result <- all.equal(peak_changes, peak_changes_ref) +peak_changes_result <- compare_rasters(peak_changes, peak_changes_ref) if (is.character(peak_changes_result)) { print(paste0("Error: ", peak_changes_result, " for peak changes.")) failure <- TRUE From b4e815204defc23b11443582f32fc97a0e633dae Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Fri, 28 Jun 2024 10:13:57 +0200 Subject: [PATCH 16/61] Increased robustness of test profile against FORCE-related flakiness --- bin/test.R | 41 +++++++++++++++++++++++++++-------------- 1 file changed, 27 insertions(+), 14 deletions(-) diff --git a/bin/test.R b/bin/test.R index 7c88bbb..c25022f 100755 --- a/bin/test.R +++ b/bin/test.R @@ -15,17 +15,30 @@ dinp <- args[1] # load package require(terra) -# function to compare raster values with tolerance -compare_rasters <- function(r1, r2, tolerance = 1e-5) { +# function to compare change directions +compare_direction <- function(r1, r2, threshold = 0.95) { - diff <- abs(values(r1) - values(r2)) - max_diff <- max(diff, na.rm = TRUE) + # get signs + s1 <- sign(r1) + s2 <- sign(r2) - if (max_diff > tolerance) { - return(paste("Max difference", max_diff, "exceeds tolerance", tolerance)) - } + # replace na's + vals1 <- subst(s1, NA, -9999) + vals2 <- subst(s2, NA, -9999) + + # Compare the signs + matches <- vals1 == vals2 + match_count <- sum(values(matches)) + total_count <- sum(!is.na(values(vals1))) - return(TRUE) + # Calculate the percentage of matches + match_percentage <- match_count / total_count + + if (match_percentage >= threshold) { + return(TRUE) + } else { + return(paste("Change directions not matching. Match percentage:", match_percentage)) + } } @@ -97,7 +110,7 @@ peak_year_of_change <- peak_rast["YEAR-OF-CHANGE"] ####################################################################### failure <- FALSE -woody_cover_changes_result <- compare_rasters(woody_cover_changes, woody_cover_changes_ref) +woody_cover_changes_result <- compare_direction(woody_cover_changes, woody_cover_changes_ref) if (is.character(woody_cover_changes_result)) { print(paste0("Error: ", woody_cover_changes_result, " for woody cover changes.")) failure <- TRUE @@ -105,7 +118,7 @@ if (is.character(woody_cover_changes_result)) { print("Woody cover change check passed.") } -woody_cover_year_of_change_result <- all.equal(woody_cover_year_of_change, woody_cover_year_of_change_ref) +woody_cover_year_of_change_result <- all.equal(woody_cover_year_of_change, woody_cover_year_of_change_ref, tolerance=1e-3) if (is.character(woody_cover_year_of_change_result)) { print(paste0("Error: ", woody_cover_year_of_change_result, " for woody cover year of change.")) failure <- TRUE @@ -114,7 +127,7 @@ if (is.character(woody_cover_year_of_change_result)) { } -herbaceous_cover_changes_result <- compare_rasters(herbaceous_cover_changes, herbaceous_cover_changes_ref) +herbaceous_cover_changes_result <- compare_direction(herbaceous_cover_changes, herbaceous_cover_changes_ref) if (is.character(herbaceous_cover_changes_result)) { print(paste0("Error: ",herbaceous_cover_changes_result, " for herbaceous cover changes.")) failure <- TRUE @@ -122,7 +135,7 @@ if (is.character(herbaceous_cover_changes_result)) { print("Herbaceous cover change check passed.") } -herbaceous_cover_year_of_change_result <- all.equal(herbaceous_cover_year_of_change, herbaceous_cover_year_of_change_ref) +herbaceous_cover_year_of_change_result <- all.equal(herbaceous_cover_year_of_change, herbaceous_cover_year_of_change_ref, tolerance=1e-3) if (is.character(herbaceous_cover_year_of_change_result)) { print(paste0("Error: ", herbaceous_cover_year_of_change_result, " for herbaceous cover year of change.")) failure <- TRUE @@ -131,7 +144,7 @@ if (is.character(herbaceous_cover_year_of_change_result)) { } -peak_changes_result <- compare_rasters(peak_changes, peak_changes_ref) +peak_changes_result <- compare_direction(peak_changes, peak_changes_ref) if (is.character(peak_changes_result)) { print(paste0("Error: ", peak_changes_result, " for peak changes.")) failure <- TRUE @@ -140,7 +153,7 @@ if (is.character(peak_changes_result)) { } -peak_year_of_change_result <- all.equal(peak_year_of_change, peak_year_of_change_ref) +peak_year_of_change_result <- all.equal(peak_year_of_change, peak_year_of_change_ref, tolerance=1e-3) if (is.character(peak_year_of_change_result)) { print(paste0("Error: ", peak_year_of_change_result, " for peak year of change.")) failure <- TRUE From f292928178fdaf06b6e631a47d4cb61881148e2e Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Fri, 28 Jun 2024 11:51:18 +0200 Subject: [PATCH 17/61] Removed only_tile parameter --- docs/output.md | 6 +++++- docs/usage.md | 10 ---------- modules/local/higher_level_force_config.nf | 4 ++-- nextflow.config | 1 - nextflow_schema.json | 5 ----- workflows/rangeland.nf | 4 +--- 6 files changed, 8 insertions(+), 22 deletions(-) diff --git a/docs/output.md b/docs/output.md index 1835f65..35dd6c5 100644 --- a/docs/output.md +++ b/docs/output.md @@ -82,7 +82,11 @@ Logs and analysis-ready-data (ARD) are generated using the [force-l2ps](https:// Higher level processing consist of two parts, generating parameter files and performing various processing task as defined in the parameter files. -Parameter files may be consulted to derive information about the specific processing task performed for a given tile. In this workflow a classification, optionally using spectral unmixing, is conducted. Next, time series analysis for different characteristics is performed. +Parameter files may be consulted to derive information about the specific processing task performed for a given tile. In this workflow, classification using spectral unmixing is performed. + +Spectral unmixing is a common technique to derive sub-pixel classification. Concretely, a set of endmember (provided using `--endmember`) is consulted to determine fractions of different types of vegetation, soil, $\ldots$ for each pixel. + +Next, time series analysis for different vegetation characteristics is performed. The resulting trend files can be consulted to view trends for individual tiles. They are saved as symlinks because of their large size. diff --git a/docs/usage.md b/docs/usage.md index 47162d4..1b53c6e 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -249,16 +249,6 @@ Start and end date can be passed using: Default values are `'1984-01-01'` for the start date and `'2006-12-31'` for the end date. -### Spectral Unmixing - -Spectral unmixing is a common technique to derive sub-pixel classification. Concretely, a set of endmember (provided using `--endmember`) is exploited to determine fractions of different types of vegetation, soil, $\ldots$ for each pixel. In this workflow, we users can enable spectral unmixing-based classification using the `only_tile` parameter. To enable spectral unmixing, user have to set the parameters to `true`, as this feature is disabled by default. - -Spectral unmixing can be enabled or disabled using: - -```bash ---endmember '[true|false]' -``` - ### Group size The `group_size` parameters can be ignored in most cases. It defines how many satellite scenes are processed together. The parameters is used to balance the tradeoff between I/O and computational capacities on individual compute nodes. By default, `group_size` is set to 100. diff --git a/modules/local/higher_level_force_config.nf b/modules/local/higher_level_force_config.nf index 3be1036..150fdd6 100644 --- a/modules/local/higher_level_force_config.nf +++ b/modules/local/higher_level_force_config.nf @@ -60,8 +60,8 @@ process HIGHER_LEVEL_CONFIG { # spectral index - sed -i "/^INDEX /c\\INDEX = SMA${params.only_tile ? ' NDVI BLUE GREEN RED NIR SWIR1 SWIR2' : ''}" \$PARAM - ${ params.only_tile ? 'sed -i "/^OUTPUT_TSS /c\\OUTPUT_TSS = TRUE" \$PARAM' : ''} + sed -i "/^INDEX /c\\INDEX = SMA NDVI BLUE GREEN RED NIR SWIR1 SWIR2" \$PARAM + sed -i "/^OUTPUT_TSS /c\\OUTPUT_TSS = TRUE" \$PARAM # interpolation sed -i "/^INT_DAY /c\\INT_DAY = 8" \$PARAM diff --git a/nextflow.config b/nextflow.config index 1957a06..9416bad 100644 --- a/nextflow.config +++ b/nextflow.config @@ -30,7 +30,6 @@ params { // Workflow configuration group_size = 100 - only_tile = false // FORCE force_threads = 2 diff --git a/nextflow_schema.json b/nextflow_schema.json index ebcce1c..64e6dfb 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -143,11 +143,6 @@ "description": "Batch size of tiles considered for merging.", "help_text": "Controls trade-off between parallelism and I/O load.\nHigher group_size -> More tiles merged in one process, less I/O load.\nLower group_size -> Less tiles merged in one process, more I/O load. ", "fa_icon": "fas fa-layer-group" - }, - "only_tile": { - "type": "boolean", - "description": "Controls wheter spectral unmixing is applied in higher level processing..", - "fa_icon": "fas fa-project-diagram" } }, "help_text": "You can savely use the defaults.", diff --git a/workflows/rangeland.nf b/workflows/rangeland.nf index f217e7a..57a8f1e 100644 --- a/workflows/rangeland.nf +++ b/workflows/rangeland.nf @@ -122,12 +122,10 @@ workflow RANGELAND { PREPROCESSING(data, dem, wvdb, cube_file, aoi_file) ch_versions = ch_versions.mix(PREPROCESSING.out.versions) - preprocessed_data = PREPROCESSING.out.tiles_and_masks.filter { params.only_tile ? it[0] == params.only_tile : true } - // // SUBWORKFLOW: Generate trend files and visualization // - HIGHER_LEVEL( preprocessed_data, cube_file, endmember_file ) + HIGHER_LEVEL( PREPROCESSING.out.tiles_and_masks, cube_file, endmember_file ) ch_versions = ch_versions.mix(HIGHER_LEVEL.out.versions) grouped_trend_data = HIGHER_LEVEL.out.trend_files.map{ it[1] }.flatten().buffer( size: Integer.MAX_VALUE, remainder: true ) From 52c93c939332da50b0b26b1765f1f58baf149006 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Wed, 24 Jul 2024 11:16:17 +0200 Subject: [PATCH 18/61] Added new options for higher level processing and visualization. --- conf/test.config | 3 ++ docs/output.md | 4 +- docs/usage.md | 31 +++++++++++++- modules/local/higher_level_force_config.nf | 4 +- nextflow.config | 16 +++++-- nextflow_schema.json | 50 ++++++++++++++++++++++ subworkflows/local/higher_level.nf | 21 ++++++--- workflows/rangeland.nf | 2 +- 8 files changed, 114 insertions(+), 17 deletions(-) diff --git a/conf/test.config b/conf/test.config index f6bd6ee..6b6e679 100644 --- a/conf/test.config +++ b/conf/test.config @@ -52,6 +52,9 @@ params { // Other parameters group_size = 10 + // enable mosaic for result checking + mosaic_visualization = true + validationSchemaIgnoreParams = "peak_yoc_ref,peak_change_ref,herbaceous_yoc_ref,herbaceous_change_ref,woody_yoc_ref,woody_change_ref,config_profile_description,config_profile_name" } diff --git a/docs/output.md b/docs/output.md index 35dd6c5..59dff46 100644 --- a/docs/output.md +++ b/docs/output.md @@ -90,6 +90,8 @@ Next, time series analysis for different vegetation characteristics is performed The resulting trend files can be consulted to view trends for individual tiles. They are saved as symlinks because of their large size. +If the `return_tss` parameter was set to `true`, the pipeline will also output files with the `TSS` ending. These files contain the time series stack(TSS) for the given tile and index or band. Here, for each date of acquisition, an image is available that contains the values for that date. + ### Visualization
    @@ -103,7 +105,7 @@ The resulting trend files can be consulted to view trends for individual tiles.
    -Two types of common visualizations are generated in the last step of the pipeline. They are results of [force-mosaic](https://force-eo.readthedocs.io/en/latest/components/auxilliary/mosaic.html) and [force-pyramid](https://force-eo.readthedocs.io/en/latest/components/auxilliary/pyramid.html). Note that these visualizations do not add more logic to the workflow but rather rearrange the output files of higher-level-processing. +Two types of common visualizations are generated in the last step of the pipeline. They are results of [force-mosaic](https://force-eo.readthedocs.io/en/latest/components/auxilliary/mosaic.html) and [force-pyramid](https://force-eo.readthedocs.io/en/latest/components/auxilliary/pyramid.html). Note that these visualizations do not add more logic to the workflow but rather rearrange the output files of higher-level-processing. Both visualizations are enabled by default but may be disabled in a certain configuration files. Thus, these outputs are optional. ### MultiQC diff --git a/docs/usage.md b/docs/usage.md index 1b53c6e..90aaca1 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -4,8 +4,6 @@ > _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ -## Introduction - ## Input As most remote sensing workflows, this pipeline relies on numerous sources of data. In the following we will describe the required data and corresponding formats. Mandatory input data consists of satellite data, a digital elevation model, a water vapor database, a data_cube, an area-of-interest specification and an endmember definition. @@ -259,6 +257,35 @@ The group size can be passed using: --group_size '[integer]' ``` +### Higher level processing configuration + +During the higher level processing stage, time series analyses of different satellite bands and indexes is performed. The concrete bands and indexes can be defined using the `indexes` parameter. Spectral unmixing is performed in any case. Thus, passing an empty `indexes` parameter will restrict time series analyses to the results of spectral unmixing. All available indexes can be found [here](https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html) above the `INDEX` parameter. The band/index codes need to be passed in a space-separated string. The default value, `indexes = "NDVI BLUE GREEN RED NIR SWIR1 SWIR2"`, enables time series analyses for the NDVI index and the blue, green, red, near-infrared and both shortwave infrared bands. Note that indexes are usually computed based on certain bands. If these bands are not present in the preprocessed data, these indexes can not be computed. + +The bands and indexes can be passed using: + +```bash +--indexes '[index-string]' +``` + +In so cases, it may be desirable to analyze the the individual images in a time series. To enable such analysis, the parameter `return_tss` can be used. If set to `true`, the pipeline will return time series stacks for each tile and band combination. The option is disabled by default to reduce the output size. + +The time series stack output can be enabled using: + +```bash +--return_tss '[boolean]' +``` + +### Visualization + +The workflow provides two types of results visualization and aggregation. The fine grained mosaic visualization contains all time series analyses results for all tiles in the original resolution. Pyramid visualizations present a broad overview of the same data but at a lower resolution. Both visualizations can be enabled or disabled using the parameters `mosaic_visualization` and `pyramid_visualization`. By default, both visualization methods are enabled. Note that the mosaic visualization is required to be enabled when using the `test` and `test_full` profiles to allow the pipeline to check the correctness of its results (this is the default behavior, make sure to not disable mosaic when using test profiles) . + +The visualizations can be enabled using: + +```bash +mosaic_visualization = '[boolean]' +pyramid_visualization = '[boolean]' +``` + ### FORCE configuration FORCE supports parallel computations. Users can specify the number of threads FORCE can spawn for a single preprocessing, or higher level processing process. This is archived through the `force_threads` parameter. diff --git a/modules/local/higher_level_force_config.nf b/modules/local/higher_level_force_config.nf index 150fdd6..43ffeef 100644 --- a/modules/local/higher_level_force_config.nf +++ b/modules/local/higher_level_force_config.nf @@ -60,8 +60,8 @@ process HIGHER_LEVEL_CONFIG { # spectral index - sed -i "/^INDEX /c\\INDEX = SMA NDVI BLUE GREEN RED NIR SWIR1 SWIR2" \$PARAM - sed -i "/^OUTPUT_TSS /c\\OUTPUT_TSS = TRUE" \$PARAM + sed -i "/^INDEX /c\\INDEX = SMA $params.indexes" \$PARAM + ${ params.return_tss ? 'sed -i "/^OUTPUT_TSS /c\\OUTPUT_TSS = TRUE" \$PARAM' : '' } # interpolation sed -i "/^INT_DAY /c\\INT_DAY = 8" \$PARAM diff --git a/nextflow.config b/nextflow.config index 9416bad..e561fb5 100644 --- a/nextflow.config +++ b/nextflow.config @@ -34,11 +34,19 @@ params { // FORCE force_threads = 2 + // Higher Level processing configuration + indexes = "NDVI BLUE GREEN RED NIR SWIR1 SWIR2" + return_tss = false + + // Visualization + mosaic_visualization = true + pyramid_visualization = true + // MultiQC options - multiqc_config = null - multiqc_title = null - multiqc_logo = null - max_multiqc_email_size = '25.MB' + multiqc_config = null + multiqc_title = null + multiqc_logo = null + max_multiqc_email_size = '25.MB' multiqc_methods_description = null // Boilerplate options diff --git a/nextflow_schema.json b/nextflow_schema.json index 64e6dfb..60372db 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -131,6 +131,50 @@ }, "fa_icon": "fas fa-satellite" }, + "higher_level_processing_modification": { + "title": "Higher level processing modification", + "type": "object", + "default": "", + "properties": { + "indexes": { + "type": "string", + "default": "NDVI BLUE GREEN RED NIR SWIR1 SWIR2", + "help_text": "Space-separated list of indexes and bands that should be considered in time series analyses. They are indicated by using their established abbreviations. The full list of available indexes is available at https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html under the 'INDEX' parameter. Spectral unmixing is a special index and always activated.", + "description": "Select which bands and indexes should be considered in time series analyses.", + "fa_icon": "fas fa-satellite" + }, + "return_tss": { + "type": "boolean", + "description": "Should the full time series stack be returned.", + "help_text": "This parameter enables or disables the output of the time series stack for each tile and band/index. Here, each band shows the values of a single date.", + "fa_icon": "fas fa-layer-group" + } + }, + "description": "Modify higher level processing behaviour.", + "help_text": "These parameters can be used to modify the behaviour of the time series analyses process in higher level processing.", + "fa_icon": "fas fa-microchip" + }, + "visualization": { + "title": "Visualization", + "type": "object", + "description": "Enable or disable visualizations.", + "default": "", + "properties": { + "mosaic_visualization": { + "type": "boolean", + "default": true, + "description": "Wheter mosaic visualization should be returned.", + "fa_icon": "fas fa-image" + }, + "pyramid_visualization": { + "type": "boolean", + "default": true, + "description": "Whether pyramid visualization should be returned.", + "fa_icon": "far fa-image" + } + }, + "fa_icon": "fas fa-images" + }, "workflow_configuration": { "title": "Workflow configuration", "type": "object", @@ -373,6 +417,12 @@ { "$ref": "#/definitions/remote_sensing_image_options" }, + { + "$ref": "#/definitions/higher_level_processing_modification" + }, + { + "$ref": "#/definitions/visualization" + }, { "$ref": "#/definitions/workflow_configuration" }, diff --git a/subworkflows/local/higher_level.nf b/subworkflows/local/higher_level.nf index ac750df..d1006ac 100644 --- a/subworkflows/local/higher_level.nf +++ b/subworkflows/local/higher_level.nf @@ -31,14 +31,21 @@ workflow HIGHER_LEVEL { trend_files_mosaic = trend_files.groupTuple() // visualizations - FORCE_MOSAIC( trend_files_mosaic, cube_file ) - ch_versions = ch_versions.mix(FORCE_MOSAIC.out.versions.first()) - - FORCE_PYRAMID( trend_files.filter { it[1].name.endsWith('.tif') }.map { [ it[1].simpleName.substring(0,11), it[1] ] } .groupTuple() ) - ch_versions = ch_versions.mix(FORCE_PYRAMID.out.versions.first()) + mosaic_files = Channel.empty() + if (params.mosaic_visualization) { + FORCE_MOSAIC( trend_files_mosaic, cube_file ) + mosaic_files = FORCE_MOSAIC.out.trend_files + ch_versions = ch_versions.mix(FORCE_MOSAIC.out.versions.first()) + } + + if (params.pyramid_visualization) { + FORCE_PYRAMID( trend_files.filter { it[1].name.endsWith('.tif') }.map { [ it[1].simpleName.substring(0,11), it[1] ] } .groupTuple() ) + ch_versions = ch_versions.mix(FORCE_PYRAMID.out.versions.first()) + } emit: - trend_files = FORCE_MOSAIC.out.trend_files - versions = ch_versions + mosaic_files + + versions = ch_versions } diff --git a/workflows/rangeland.nf b/workflows/rangeland.nf index 57a8f1e..fb44c42 100644 --- a/workflows/rangeland.nf +++ b/workflows/rangeland.nf @@ -128,7 +128,7 @@ workflow RANGELAND { HIGHER_LEVEL( PREPROCESSING.out.tiles_and_masks, cube_file, endmember_file ) ch_versions = ch_versions.mix(HIGHER_LEVEL.out.versions) - grouped_trend_data = HIGHER_LEVEL.out.trend_files.map{ it[1] }.flatten().buffer( size: Integer.MAX_VALUE, remainder: true ) + grouped_trend_data = HIGHER_LEVEL.out.mosaic_files.map{ it[1] }.flatten().buffer( size: Integer.MAX_VALUE, remainder: true ) // // MODULE: Check results From a4d297f7c79f17ac8f19f858e9509f7a69b72fc4 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Wed, 31 Jul 2024 20:18:46 +0200 Subject: [PATCH 19/61] Added test_full profile --- bin/test.R | 47 ++++++++++++++++++++++------- conf/test_full.config | 47 +++++++++++++++++++++++++---- modules/local/check_results_full.nf | 36 ++++++++++++++++++++++ workflows/rangeland.nf | 24 ++++++++++----- 4 files changed, 130 insertions(+), 24 deletions(-) create mode 100644 modules/local/check_results_full.nf diff --git a/bin/test.R b/bin/test.R index c25022f..2c5b0d5 100755 --- a/bin/test.R +++ b/bin/test.R @@ -3,15 +3,17 @@ args = commandArgs(trailingOnly=TRUE) -if (length(args) != 7) { - stop("\ngive input directory (mosaic) as 1st arg\ngive reference rasters (*.tif) as 2nd-7th args in order: +if (length(args) != 7 && length(args) != 2) { + stop("\n Error: wrong number of parameters. Usage: \n 1st arg: workflow results directory (mosaic) + \n 2nd-7th args: reference rasters (*.tif) in order: woody cover change, woody cover year of change, herbaceous cover change, herbaceous cover year of change, - peak change, peak year of change, ", call.=FALSE) + peak change, peak year of change + \nor \n + 1st arg: workflow results directory (mosaic) \n 2nd arg: reference directory + ", call.=FALSE) } -dinp <- args[1] - # load package require(terra) @@ -44,19 +46,42 @@ compare_direction <- function(r1, r2, threshold = 0.95) { # LOAD REFERENCE ####################################################################### -woody_cover_changes_ref <- rast(args[2]) -woody_cover_year_of_change_ref <- rast(args[3]) -herbaceous_cover_changes_ref <- rast(args[4]) -herbaceous_cover_year_of_change_ref <- rast(args[5]) +if (length(args) == 7 ){ + woody_cover_changes_ref <- rast(args[2]) + woody_cover_year_of_change_ref <- rast(args[3]) -peak_changes_ref <- rast(args[6]) -peak_year_of_change_ref <- rast(args[7]) + herbaceous_cover_changes_ref <- rast(args[4]) + herbaceous_cover_year_of_change_ref <- rast(args[5]) + peak_changes_ref <- rast(args[6]) + peak_year_of_change_ref <- rast(args[7]) +} else { + # reference parent dir + ref_dir <- args[2] + + vrt_file <- list.files(ref_dir, pattern = "VBL-CAO\\.vrt$", recursive = TRUE, full.names = TRUE) + woody_ref <- rast(vrt_file) + woody_cover_changes_ref <- woody_ref$CHANGE + woody_cover_year_of_change_ref <- woody_ref["YEAR-OF-CHANGE"] + + vrt_file <- list.files(ref_dir, pattern = "VSA-CAO\\.vrt$", recursive = TRUE, full.names = TRUE) + herbaceous_ref <- rast(vrt_file) + herbaceous_cover_changes_ref <- herbaceous_ref$CHANGE + herbaceous_cover_year_of_change_ref <- herbaceous_ref["YEAR-OF-CHANGE"] + + vrt_file <- list.files(ref_dir, pattern = "VPS-CAO\\.vrt$", recursive = TRUE, full.names = TRUE) + peak_ref <- rast(vrt_file) + peak_changes_ref <- peak_ref$CHANGE + peak_year_of_change_ref <- peak_ref["YEAR-OF-CHANGE"] +} # WOODY COVER CHANGE (VALUE OF BASE LEVEL) ####################################################################### +# input data dir +dinp <- args[1] + fname <- dir(dinp, ".*HL_TSA_LNDLG_SMA_VBL-CAO.vrt$", full.names=TRUE) woody_cover_rast <- rast(fname) diff --git a/conf/test_full.config b/conf/test_full.config index 024a55c..907fdf8 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -14,11 +14,46 @@ params { config_profile_name = 'Full test profile' config_profile_description = 'Full test dataset to check pipeline function' - // Input data for full size test - // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) - // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' + // Input data + input = 's3://ngi-igenomes/test-data/rangeland/landsat.tar' + dem = 's3://ngi-igenomes/test-data/rangeland/dem.tar' + wvdb = 's3://ngi-igenomes/test-data/rangeland/wvdb.tar' - // Fasta references - fasta = params.pipelines_testdata_base_path + 'viralrecon/genome/NC_045512.2/GCF_009858895.2_ASM985889v3_genomic.200409.fna.gz' + input_tar = true + dem_tar = true + wvdb_tar = true + + data_cube = 's3://ngi-igenomes/test-data/rangeland/datacube-definition.prj' + aoi = 's3://ngi-igenomes/test-data/rangeland/aoi.gpkg' + endmember = 's3://ngi-igenomes/test-data/rangeland/hostert-2003.txt' + + + // Remote sensing imagery parameters + start_date = '1986-01-01' + end_date = '1989-12-31' + + sensors_level1 = 'LT04,LT05' + sensors_level2 = 'LND04 LND05' + + // enable time series stack output + return_tss = true + + // enable mosaic for result checking (required for result checking) + mosaic_visualization = true + + // reference data + reference = "s3://ngi-igenomes/test-data/rangeland/reference.tar" + + validationSchemaIgnoreParams = "reference" + +} + +process { + withName: "UNTAR_*" { + container = 'docker.io/ubuntu:23.10' + ext.args2 = "--strip-components=1" + } + withName: "UNTAR_REF"{ + ext.args2 = "--strip-components=0" + } } diff --git a/modules/local/check_results_full.nf b/modules/local/check_results_full.nf new file mode 100644 index 0000000..c57175a --- /dev/null +++ b/modules/local/check_results_full.nf @@ -0,0 +1,36 @@ +nextflow.enable.dsl = 2 + +process CHECK_RESULTS_FULL { + + label 'process_low' + + container 'docker.io/rocker/geospatial:4.3.1' + + input: + path{ "trend/?/*" } + path reference + + output: + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + files=`find ./trend/ -maxdepth 1 -mindepth 1 -type d` + for path in \$files; do + mkdir -p trend/\$(ls \$path) + cp \$path/*/* trend/\$(ls \$path)/ + rm \$path -r + done; + test.R trend/mosaic $reference + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + r-base: \$(echo \$(R --version 2>&1) | sed 's/^.*R version //; s/ .*\$//') + terra: \$(Rscript -e "library(terra); cat(as.character(packageVersion('terra')))") + END_VERSIONS + """ + +} diff --git a/workflows/rangeland.nf b/workflows/rangeland.nf index fb44c42..08cda4d 100644 --- a/workflows/rangeland.nf +++ b/workflows/rangeland.nf @@ -26,8 +26,8 @@ include { HIGHER_LEVEL } from '../subworkflows/local/higher_level' // MODULES // -include { CHECK_RESULTS } from '../modules/local/check_results' - +include { CHECK_RESULTS } from '../modules/local/check_results' +include { CHECK_RESULTS_FULL } from '../modules/local/check_results_full' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT NF-CORE MODULES/SUBWORKFLOWS @@ -37,7 +37,7 @@ include { CHECK_RESULTS } from '../modules/local/check_results' // // MODULE: Installed directly from nf-core/modules // -include { UNTAR as UNTAR_INPUT; UNTAR as UNTAR_DEM; UNTAR as UNTAR_WVDB } from '../modules/nf-core/untar/main' +include { UNTAR as UNTAR_INPUT; UNTAR as UNTAR_DEM; UNTAR as UNTAR_WVDB; UNTAR as UNTAR_REF } from '../modules/nf-core/untar/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -114,7 +114,6 @@ workflow RANGELAND { wvdb = file("$params.wvdb") } - ch_versions = ch_versions.mix(tar_versions.first()) // // SUBWORKFLOW: Preprocess satellite imagery @@ -125,7 +124,7 @@ workflow RANGELAND { // // SUBWORKFLOW: Generate trend files and visualization // - HIGHER_LEVEL( PREPROCESSING.out.tiles_and_masks, cube_file, endmember_file ) + HIGHER_LEVEL(PREPROCESSING.out.tiles_and_masks, cube_file, endmember_file) ch_versions = ch_versions.mix(HIGHER_LEVEL.out.versions) grouped_trend_data = HIGHER_LEVEL.out.mosaic_files.map{ it[1] }.flatten().buffer( size: Integer.MAX_VALUE, remainder: true ) @@ -133,7 +132,7 @@ workflow RANGELAND { // // MODULE: Check results // - if ( params.config_profile_name == 'Test profile' ) { + if (params.config_profile_name == 'Test profile') { woody_change_ref = file("$params.woody_change_ref") woody_yoc_ref = file("$params.woody_yoc_ref") herbaceous_change_ref = file("$params.herbaceous_change_ref") @@ -141,10 +140,21 @@ workflow RANGELAND { peak_change_ref = file("$params.peak_change_ref") peak_yoc_ref = file("$params.peak_yoc_ref") - CHECK_RESULTS( grouped_trend_data, woody_change_ref, woody_yoc_ref, herbaceous_change_ref, herbaceous_yoc_ref, peak_change_ref, peak_yoc_ref) + CHECK_RESULTS(grouped_trend_data, woody_change_ref, woody_yoc_ref, herbaceous_change_ref, herbaceous_yoc_ref, peak_change_ref, peak_yoc_ref) ch_versions = ch_versions.mix(CHECK_RESULTS.out.versions) } + if (params.config_profile_name == 'Full test profile') { + UNTAR_REF([[:], params.reference]) + ref_path = UNTAR_REF.out.untar.map(it -> it[1]) + tar_versions.mix(UNTAR_REF.out.versions) + + CHECK_RESULTS_FULL(grouped_trend_data, ref_path) + ch_versions = ch_versions.mix(CHECK_RESULTS_FULL.out.versions) + } + + ch_versions = ch_versions.mix(tar_versions.first()) + // // Collate and save software versions // From b896dda350bb94c162b5089f7626ddbe1a291885 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Wed, 31 Jul 2024 20:24:35 +0200 Subject: [PATCH 20/61] bumped version to 1.0.0 --- assets/multiqc_config.yml | 4 ++-- nextflow.config | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index d2ae102..13dd96a 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/rangeland + This report has been generated by the nf-core/rangeland analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-rangeland-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index e561fb5..4113199 100644 --- a/nextflow.config +++ b/nextflow.config @@ -260,7 +260,7 @@ manifest { description = """""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.0dev' + version = '1.0.0' doi = '' } From 443ba0aada2de1622197b2b52cda8d485f973d2b Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Wed, 7 Aug 2024 15:11:40 +0200 Subject: [PATCH 21/61] Implemented minor reviewer requests --- README.md | 5 ++- bin/merge_boa.r | 5 ++- bin/merge_qai.r | 5 ++- bin/test.R | 15 -------- docs/output.md | 12 +++---- docs/usage.md | 36 +++++++++---------- modules/local/check_results.nf | 2 -- modules/local/check_results_full.nf | 2 -- modules/local/force-generate_analysis_mask.nf | 2 -- .../local/force-generate_tile_allow_list.nf | 2 -- modules/local/force-higher_level.nf | 2 -- modules/local/force-mosaic.nf | 2 -- modules/local/force-preprocess.nf | 2 -- modules/local/force-pyramid.nf | 2 -- modules/local/higher_level_force_config.nf | 2 -- modules/local/merge.nf | 2 -- modules/local/preprocess_force_config.nf | 2 -- nextflow.config | 20 +++++------ nextflow_schema.json | 22 ++++++------ subworkflows/local/higher_level.nf | 3 -- subworkflows/local/preprocessing.nf | 5 +-- 21 files changed, 50 insertions(+), 100 deletions(-) diff --git a/README.md b/README.md index e5516b9..a95323f 100644 --- a/README.md +++ b/README.md @@ -28,8 +28,7 @@ 5. Time series analyses to obtain trends in vegetation dynamics 6. Create mosaic and pyramid visualizations of the results -7. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) -8. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) +7. Present QC results ([`MultiQC`](http://multiqc.info/)) ## Usage @@ -41,7 +40,7 @@ To run the pipeline on real data, input data needs to be acquired. Concretely, s Now, you can run the pipeline using: ```bash -nextflow run nf-core/rangeland/main.nf \ +nextflow run nf-core/rangeland \ -profile \ --input \ --dem \ diff --git a/bin/merge_boa.r b/bin/merge_boa.r index 27779ad..622ba08 100755 --- a/bin/merge_boa.r +++ b/bin/merge_boa.r @@ -1,5 +1,7 @@ #!/usr/bin/env Rscript +require(raster) + args = commandArgs(trailingOnly=TRUE) @@ -11,9 +13,6 @@ fout <- args[1] finp <- args[2:length(args)] nf <- length(finp) -require(raster) - - img <- brick(finp[1]) nc <- ncell(img) nb <- nbands(img) diff --git a/bin/merge_qai.r b/bin/merge_qai.r index fc9066f..99f2cff 100755 --- a/bin/merge_qai.r +++ b/bin/merge_qai.r @@ -1,5 +1,7 @@ #!/usr/bin/env Rscript +require(raster) + args = commandArgs(trailingOnly=TRUE) @@ -11,9 +13,6 @@ fout <- args[1] finp <- args[2:length(args)] nf <- length(finp) -require(raster) - - img <- raster(finp[1]) nc <- ncell(img) diff --git a/bin/test.R b/bin/test.R index 2c5b0d5..173cdbb 100755 --- a/bin/test.R +++ b/bin/test.R @@ -116,21 +116,6 @@ peak_year_of_change <- peak_rast["YEAR-OF-CHANGE"] -# FOR REFERENCE: SAVE RASTERS -####################################################################### - -#writeRaster(woody_cover_changes, "woody_cover_chg_ref.tif") -#writeRaster(woody_cover_year_of_change, "woody_cover_yoc_ref.tif") - -#writeRaster(herbaceous_cover_changes, "herbaceous_cover_chg_ref.tif") -#writeRaster(herbaceous_cover_year_of_change, "herbaceous_cover_yoc_ref.tif") - -#writeRaster(peak_changes, "peak_chg_ref.tif") -#writeRaster(peak_year_of_change, "peak_yoc_ref.tif") - - - - # COMPARE TESTRUN WITH REFERENCE EXECUTION ####################################################################### failure <- FALSE diff --git a/docs/output.md b/docs/output.md index 59dff46..f4e86f3 100644 --- a/docs/output.md +++ b/docs/output.md @@ -32,7 +32,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d [untar](https://nf-co.re/modules/untar) is a nf-core module used to extract files from tar archives. -Invokation of untar depends on certain parameters (i.e input_tar, dem_tar and wvdb_tar). Thus, the outputs files are only generated when these are set to true. +Invokation of untar depends on certain parameters (i.e `input_tar`, `dem_tar` and `wvdb_tar`). Thus, the outputs files are only generated when these are set to `true`. ### Preparation @@ -76,7 +76,7 @@ Logs and analysis-ready-data (ARD) are generated using the [force-l2ps](https:// - `higher-level//` - `param_files/`: Parameter files used in [force-higher-level](https://force-eo.readthedocs.io/en/latest/components/higher-level/index.html). - - `trend_files`: Symlinks to trend files that are the result of higher-level processing. + - `trend_files/`: Symlinks to trend files that are the result of higher-level processing. @@ -84,7 +84,7 @@ Higher level processing consist of two parts, generating parameter files and per Parameter files may be consulted to derive information about the specific processing task performed for a given tile. In this workflow, classification using spectral unmixing is performed. -Spectral unmixing is a common technique to derive sub-pixel classification. Concretely, a set of endmember (provided using `--endmember`) is consulted to determine fractions of different types of vegetation, soil, $\ldots$ for each pixel. +Spectral unmixing is a common technique to derive sub-pixel classification. Concretely, a set of endmember (provided using `--endmember`) is consulted to determine fractions of different types of vegetation, soil, ... for each pixel. Next, time series analysis for different vegetation characteristics is performed. @@ -99,8 +99,8 @@ If the `return_tss` parameter was set to `true`, the pipeline will also output f - `trend/` - `mosaic//` - - ``: Auxiliary files for the mosaic visualization. - - `mosaic`: Contains a single virtual raster file that defines the mosaic visualization. + - `/`: .tif files that are part of the mosaic. + - `mosaic/`: Contains a single virtual raster file that combines the .tif files into the mosaic visualization. - `pyramid//trend//`: Contains tile-wise pyramid visualizations for every trend analyzed in the workflow. @@ -121,7 +121,7 @@ Two types of common visualizations are generated in the last step of the pipelin [MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. -Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . +Results generated by MultiQC collate pipeline QC from supported tools. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . ### Pipeline information diff --git a/docs/usage.md b/docs/usage.md index 90aaca1..5413516 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -16,7 +16,7 @@ For infos on Landsat, see [here](https://www.usgs.gov/core-science-systems/nli/l Satellite data should be given as a path to a common root of all imagery. This is a common format used in geographic information systems, including FORCE, which is applied in this pipeline. The expected structure underneath the root directory should follow this example: -``` +```tree root ├── 181035 │ └── LE07_L1TP_181035_20061217_20170106_01_T1 @@ -53,7 +53,7 @@ root └── ... ``` -Subdirectories of root contain _path_ and _row_ information as commonly used for Landsat imagery. As an example, the sub directory `181036/` contains imagery for path 18 and row 1036. +Subdirectories of `root/` contain _path_ and _row_ information as commonly used for Landsat imagery. As an example, the sub directory `181036/` contains imagery for path 18 and row 1036. The next level of subdirectories contains the data for a specific day and from a specific source. Lets look at the example `LE07_L1TP_181036_20061217_20170105_01_T1`: @@ -68,14 +68,14 @@ The next level of subdirectories contains the data for a specific day and from a On the lowest level of the structure, the actual data is stored. Looking at the contents of `LE07_L1TP_181036_20061217_20170105_01_T1`, we see that all files share the same prefix, followed by a specification of the specific files contents. These suffixes include: - "B" followed by a number _i_ identifying the band of the satellite (band 6 has two files as Landsat 7 has two thermal bands) -- "BQA" identifying the quality information band +- "BQA" identifies the quality information band - "GCP" identifies ground control point information - "ANG" identifies angle of observation and other geometric information information - "MTL" identifies meta data -All files within the lowest level of structure belong to a single observation. Files containing imagery (prefix starts with "B") should be .tif files. Files containing auxiliary data are text files. +All files within the lowest level of structure belong to a single observation. Files containing imagery (prefix starts with "B") should be `.tif` files. Files containing auxiliary data are text files. -This structure is automatically generated when [using force to download the data](https://force-eo.readthedocs.io/en/latest/components/lower-level/level1/level1-csd.html?). We strongly suggest users to download data using FORCE (e.g.). For example, executing the following code (e.g. with [FORCE in docker](https://force-eo.readthedocs.io/en/latest/setup/docker.html)) will download data for Landsat 4,5 and 7, in the time range from 1st January 1984 until 31st December 2006, including pictures with up to 70 percent of cloud coverage: +This structure is automatically generated when [using force to download the data](https://force-eo.readthedocs.io/en/latest/components/lower-level/level1/level1-csd.html?). We strongly suggest users to download data using FORCE. For example, executing the following code (e.g. with [FORCE in docker](https://force-eo.readthedocs.io/en/latest/setup/docker.html)) will download data for Landsat 4,5 and 7, in the time range from 1st January 1984 until 31st December 2006, including pictures with up to 70 percent of cloud coverage: ```bash mkdir -p meta @@ -84,7 +84,7 @@ mkdir -p data force-level1-csd -s "LT04,LT05,LE07" -d "19840101,20061231" -c 0,70 meta/ data/ queue.txt vector/aoi.gpkg ``` -Note that you need to pass an area-of-interest file, see the area of interest section [Area of interest](#aoi) for details. +Note that you need to pass an area-of-interest file, see the area of interest section [Area of interest](#area-of-interest-aoi) for details. The satellite imagery can be given to the pipeline using: @@ -92,7 +92,7 @@ The satellite imagery can be given to the pipeline using: --input '[path to imagery root]' ``` -The satellite imagery can also be provide as a tar archive. In this case it is mandatory to set `--input_tar` to true. Moreover, within the tar archive, the structure explained above has to be in place. In the example above `181036/` and `181035/` would need to be in the top level of the archive. +The satellite imagery can also be provide as a tar archive. In this case it is mandatory to set `--input_tar` to `true`. Moreover, within the tar archive, the structure explained above has to be in place. In the example above `181036/` and `181035/` would need to be in the top level of the archive. ### Digital Elevation Model (DEM) @@ -100,7 +100,7 @@ A DEM is necessary for topographic correction of Landsat data, and helps to dist The pipeline expects a path to the Digital Elevation Model root directory as a parameter. Concretely, the expected structure would look like this: -``` +```tree dem ├── .vrt └── / @@ -115,7 +115,7 @@ The DEM can be given to the pipeline using: --dem '[path to dem root]' ``` -The digital elevation model can also be provide as a tar archive. In this case it is mandatory to set `--dem_tar` to true. Moreover, within the tar archive, the structure explained above has to be in place. In the example above `.vrt` and `/` would need to be in the top level of the archive. +The digital elevation model can also be provide as a tar archive. In this case it is mandatory to set `--dem_tar` to `true`. Moreover, within the tar archive, the structure explained above has to be in place. In the example above `.vrt` and `/` would need to be in the top level of the archive. ### Water Vapor Database (WVDB) @@ -138,7 +138,7 @@ The WVDB can be given to the pipeline using: --wvdb '[path to wvdb dir]' ``` -The water vapor database can also be provide as a tar archive. In this case it is mandatory to set `--wvdb_tar` to true. All files of the wvdb would need to be in the top level of the archive. +The water vapor database can also be provide as a tar archive. In this case it is mandatory to set `--wvdb_tar` to `true`. All files of the wvdb would need to be in the top level of the archive. ### Datacube @@ -152,11 +152,9 @@ The datacube definition is passed as a single file using: ### Area of interest (AOI) - - The area of interest is a geospatial vector dataset that holds the boundary of the targeted area. -AOI is passed as a single using: +AOI is passed as a single file using: ```bash --aoi '[path to area of interest file]' @@ -168,7 +166,7 @@ For unmixing satellite-observed reflectance into sub-pixel fractions of land sur An example endmember definition (developed in [Hostert et al. 2003](https://www.sciencedirect.com/science/article/abs/pii/S0034425703001457)) looks like this: -``` +```tsv 320 730 2620 0 560 1450 3100 0 450 2240 3340 0 @@ -213,7 +211,7 @@ More details on available satellite identifiers can be found [here](https://forc Note that the identifiers specified for both processing levels have to match the data made available to the workflow. In other words, satellite data for e.g. Landsat 5 can't be processed if it was not supplied using the `input` parameter. -Both parameters can be passed as using: +Both parameters can be passed as follows: ```bash --sensors_level1 = '[preprocessing satellite identifier string]' @@ -232,7 +230,7 @@ A custom resolution can be passed using: --resolution '[integer]' ``` -The default value is 30, as most Landsat satellite natively provide this resolution. +The default value is `30`, as most Landsat satellite natively provide this resolution. ### Temporal extent @@ -245,11 +243,9 @@ Start and end date can be passed using: --end_date '[YYYY-MM-DD]' ``` -Default values are `'1984-01-01'` for the start date and `'2006-12-31'` for the end date. - ### Group size -The `group_size` parameters can be ignored in most cases. It defines how many satellite scenes are processed together. The parameters is used to balance the tradeoff between I/O and computational capacities on individual compute nodes. By default, `group_size` is set to 100. +The `group_size` parameters can be ignored in most cases. It defines how many satellite scenes are processed together. The parameters is used to balance the tradeoff between I/O and computational capacities on individual compute nodes. By default, `group_size` is set to `100`. The group size can be passed using: @@ -277,7 +273,7 @@ The time series stack output can be enabled using: ### Visualization -The workflow provides two types of results visualization and aggregation. The fine grained mosaic visualization contains all time series analyses results for all tiles in the original resolution. Pyramid visualizations present a broad overview of the same data but at a lower resolution. Both visualizations can be enabled or disabled using the parameters `mosaic_visualization` and `pyramid_visualization`. By default, both visualization methods are enabled. Note that the mosaic visualization is required to be enabled when using the `test` and `test_full` profiles to allow the pipeline to check the correctness of its results (this is the default behavior, make sure to not disable mosaic when using test profiles) . +The workflow provides two types of results visualization and aggregation. The fine grained mosaic visualization contains all time series analyses results for all tiles in the original resolution. Pyramid visualizations present a broad overview of the same data but at a lower resolution. Both visualizations can be enabled or disabled using the parameters `mosaic_visualization` and `pyramid_visualization`. By default, both visualization methods are enabled. Note that the mosaic visualization is required to be enabled when using the `test` and `test_full` profiles to allow the pipeline to check the correctness of its results. The visualizations can be enabled using: diff --git a/modules/local/check_results.nf b/modules/local/check_results.nf index cb57366..28cc76b 100644 --- a/modules/local/check_results.nf +++ b/modules/local/check_results.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl = 2 - process CHECK_RESULTS { label 'process_low' diff --git a/modules/local/check_results_full.nf b/modules/local/check_results_full.nf index c57175a..f47f6f8 100644 --- a/modules/local/check_results_full.nf +++ b/modules/local/check_results_full.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl = 2 - process CHECK_RESULTS_FULL { label 'process_low' diff --git a/modules/local/force-generate_analysis_mask.nf b/modules/local/force-generate_analysis_mask.nf index b5ee8b4..fa0f711 100644 --- a/modules/local/force-generate_analysis_mask.nf +++ b/modules/local/force-generate_analysis_mask.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl = 2 - process FORCE_GENERATE_ANALYSIS_MASK{ label 'process_single' diff --git a/modules/local/force-generate_tile_allow_list.nf b/modules/local/force-generate_tile_allow_list.nf index 4ea0e19..9f9ad5b 100644 --- a/modules/local/force-generate_tile_allow_list.nf +++ b/modules/local/force-generate_tile_allow_list.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl = 2 - process FORCE_GENERATE_TILE_ALLOW_LIST{ label 'process_single' diff --git a/modules/local/force-higher_level.nf b/modules/local/force-higher_level.nf index c91d9b4..0300564 100644 --- a/modules/local/force-higher_level.nf +++ b/modules/local/force-higher_level.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl = 2 - process FORCE_HIGHER_LEVEL { label 'process_medium' diff --git a/modules/local/force-mosaic.nf b/modules/local/force-mosaic.nf index 2465b07..aa37395 100644 --- a/modules/local/force-mosaic.nf +++ b/modules/local/force-mosaic.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl = 2 - process FORCE_MOSAIC{ label 'process_low' diff --git a/modules/local/force-preprocess.nf b/modules/local/force-preprocess.nf index c28af6a..dc740df 100644 --- a/modules/local/force-preprocess.nf +++ b/modules/local/force-preprocess.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl=2 - process FORCE_PREPROCESS { label 'process_medium' diff --git a/modules/local/force-pyramid.nf b/modules/local/force-pyramid.nf index e6eb9c6..4eca596 100644 --- a/modules/local/force-pyramid.nf +++ b/modules/local/force-pyramid.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl = 2 - process FORCE_PYRAMID { label 'process_low' diff --git a/modules/local/higher_level_force_config.nf b/modules/local/higher_level_force_config.nf index 43ffeef..d22ee7a 100644 --- a/modules/local/higher_level_force_config.nf +++ b/modules/local/higher_level_force_config.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl = 2 - process HIGHER_LEVEL_CONFIG { label 'process_single' diff --git a/modules/local/merge.nf b/modules/local/merge.nf index 55b0d52..0b8949c 100644 --- a/modules/local/merge.nf +++ b/modules/local/merge.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl = 2 - process MERGE { label 'process_low' diff --git a/modules/local/preprocess_force_config.nf b/modules/local/preprocess_force_config.nf index e759ac8..497966a 100644 --- a/modules/local/preprocess_force_config.nf +++ b/modules/local/preprocess_force_config.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl = 2 - process PREPROCESS_CONFIG { label 'process_single' diff --git a/nextflow.config b/nextflow.config index 4113199..25fda03 100644 --- a/nextflow.config +++ b/nextflow.config @@ -10,12 +10,12 @@ params { // Input options - input = null - dem = null - wvdb = null - data_cube = null - aoi = null - endmember = null + input = null + dem = null + wvdb = null + data_cube = null + aoi = null + endmember = null input_tar = false dem_tar = false @@ -29,7 +29,7 @@ params { end_date = "2006-12-31" // Workflow configuration - group_size = 100 + group_size = 100 // FORCE force_threads = 2 @@ -71,9 +71,9 @@ params { // Max resource options // Defaults only, expecting to be overwritten - max_memory = '128.GB' - max_cpus = 16 - max_time = '240.h' + max_memory = '128.GB' + max_cpus = 16 + max_time = '240.h' // Schema validation default options validationFailUnrecognisedParams = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 60372db..0112fb5 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -15,45 +15,45 @@ "input": { "type": "string", "fa_icon": "fas fa-satellite", - "help_text": "Set this to the root directory of all satellite imagery. Directory structure should match format of data downloaded with '$ force-level1-csd\n' For concrete directory structure see ./docs/usage.md ", + "help_text": "Set this to the root directory of all satellite imagery. Directory structure should match format of data downloaded with '$ force-level1-csd\n' For concrete directory structure see [usage documentation](https://nf-co.re/rangeland/docs/usage)", "description": "Root directory of all sattelite imagery.", "mimetype": "application/x-tar" }, "input_tar": { "type": "boolean", "fa_icon": "fas fa-archive", - "description": "Indicates whether input is a tar archive.", - "help_text": "Set to true if input is a tar archive. The workflow will extract it then." + "description": "Indicates whether `input` is a tar archive.", + "help_text": "Set to `true` if `input` is a tar archive. The workflow will extract it then." }, "dem": { "type": "string", - "help_text": "Path to directory containing a subdirectory with tile-wise digital elevation files (.tif) and a virtual dataset file (.vrt file) comprising all tile-wise files from the subdirectory. \n\n\n", + "help_text": "Path to directory containing a subdirectory with tile-wise digital elevation files (`.tif`) and a virtual dataset file (`.vrt` file) comprising all tile-wise files from the subdirectory.", "fa_icon": "fas fa-mountain", "description": "Digital elevation model." }, "dem_tar": { "type": "boolean", "fa_icon": "fas fa-archive", - "description": "Indicates whether dem is a tar archive.", - "help_text": "Set to true if dem is a tar archive. The workflow will extract it then." + "description": "Indicates whether `dem` is a tar archive.", + "help_text": "Set to `true` if `dem` is a tar archive. The workflow will extract it then." }, "wvdb": { "type": "string", "fa_icon": "fas fa-burn", "description": "Water vapor dataset.", - "help_text": "Directory containg a number text files describing global water vapor data at different timestamps, and a coordinate order (.coo-)file containig the reference system of the water vapor data." + "help_text": "Directory containg a number text files describing global water vapor data at different timestamps, and a coordinate order (`.coo`-)file containig the reference system of the water vapor data." }, "wvdb_tar": { "type": "boolean", "fa_icon": "fas fa-archive", - "description": "Indicates whether wvdb is a tar archive.", - "help_text": "Set to true if wvdb is a tar archive. The workflow will extract it then." + "description": "Indicates whether `wvdb` is a tar archive.", + "help_text": "Set to `true` if `wvdb` is a tar archive. The workflow will extract it then." }, "data_cube": { "type": "string", "description": "Datacube definition.", "fa_icon": "fas fa-cube", - "help_text": "A single .prj file describing the projection and reference grid of the generated datacube." + "help_text": "A single `.prj` file describing the projection and reference grid of the generated datacube." }, "aoi": { "type": "string", @@ -65,7 +65,7 @@ "type": "string", "fa_icon": "fas fa-chart-line", "description": "Endmember definition.", - "help_text": "A single text file specifying where lines correspond to satellite bands and columns correspond to endmembers. Values correspond to reflectance values and are separated by spaces." + "help_text": "A single text file specifying where rows correspond to satellite bands and columns correspond to endmembers. Values correspond to reflectance values and are separated by spaces." }, "outdir": { "type": "string", diff --git a/subworkflows/local/higher_level.nf b/subworkflows/local/higher_level.nf index d1006ac..fb3bd3b 100644 --- a/subworkflows/local/higher_level.nf +++ b/subworkflows/local/higher_level.nf @@ -1,6 +1,3 @@ -nextflow.enable.dsl = 2 - -//inputs include { HIGHER_LEVEL_CONFIG } from '../../modules/local/higher_level_force_config.nf' include { FORCE_HIGHER_LEVEL } from '../../modules/local/force-higher_level.nf' include { FORCE_MOSAIC } from '../../modules/local/force-mosaic.nf' diff --git a/subworkflows/local/preprocessing.nf b/subworkflows/local/preprocessing.nf index 89766c0..98ce8eb 100644 --- a/subworkflows/local/preprocessing.nf +++ b/subworkflows/local/preprocessing.nf @@ -1,13 +1,10 @@ -nextflow.enable.dsl = 2 - -//includes include { FORCE_GENERATE_TILE_ALLOW_LIST } from '../../modules/local/force-generate_tile_allow_list' include { FORCE_GENERATE_ANALYSIS_MASK } from '../../modules/local/force-generate_analysis_mask' include { PREPROCESS_CONFIG } from '../../modules/local/preprocess_force_config' include { FORCE_PREPROCESS } from '../../modules/local/force-preprocess' include { MERGE as MERGE_BOA; MERGE as MERGE_QAI } from '../../modules/local/merge' -//Closure to extract the parent directory of a file +// Closure to extract the parent directory of a file def extractDirectory = { it.parent.toString().substring(it.parent.toString().lastIndexOf('/') + 1 ) } workflow PREPROCESSING { From 3dca3917cf953f5deddb6e90a1c505a0010651ce Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Thu, 8 Aug 2024 15:59:43 +0200 Subject: [PATCH 22/61] Added parameters to control publishing behavior for intermediate processing results, some minor adjustments in the docs. --- conf/modules.config | 9 +++++---- docs/output.md | 10 +++++----- docs/usage.md | 11 +++++++++++ nextflow.config | 2 ++ nextflow_schema.json | 12 ++++++++++++ 5 files changed, 35 insertions(+), 9 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index e63e00e..de1c7e4 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -45,8 +45,9 @@ process { ], [ path: { "${params.outdir}/preprocess/${task.tag}" }, - mode: 'symlink', - pattern: 'level2_ard/**/*' + mode: params.publish_dir_mode, + pattern: 'level2_ard/**/*', + saveAs: { params.save_ard ? it : null } ] ] } @@ -65,9 +66,9 @@ process { withName: "FORCE_HIGHER_LEVEL" { publishDir = [ path: { "${params.outdir}/higher-level/${task.tag}" }, - mode: 'symlink', + mode: params.publish_dir_mode, pattern: 'trend/*.tif', - saveAs: { "trend_files/${it.tokenize('/')[-1]}" } + saveAs: { params.save_ard ? "trend_files/${it.tokenize('/')[-1]}" : null } ] } diff --git a/docs/output.md b/docs/output.md index f4e86f3..c32f660 100644 --- a/docs/output.md +++ b/docs/output.md @@ -11,10 +11,10 @@ The directories listed below will be created in the results directory after the The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: - [untar](#untar) - Optionally extract input files -- [Preparation](#preparation)- Create a masks and boundaries for further analyses. +- [Preparation](#preparation) - Create a masks and boundaries for further analyses. - [Preprocessing](#preprocessing) - Preprocessing of satellite imagery. - [Higher-level-Processing](#higher-level-processing) - Classify preprocessed imagery and perform time series analyses. -- [Visualization](#visualization). Create two visualizations of the results. +- [Visualization](#visualization) - Create two visualizations of the results. - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution @@ -67,7 +67,7 @@ Preprocessing consist of two parts, generating parameter files and actual prepro The parameter files created through [force-parameter](https://force-eo.readthedocs.io/en/latest/components/auxilliary/parameter.html#force-parameter) can be viewed to understand concrete preprocessing techniques applied for a given tile. -Logs and analysis-ready-data (ARD) are generated using the [force-l2ps](https://force-eo.readthedocs.io/en/latest/components/lower-level/level2/l2ps.html) command. Logs can be consulted for debugging purposes. ARD may be collected as a basis for other remote sensing workflows. Moreover, ARD contains two .tif files per initial input image, a quality data file and the atmospherically corrected satellite data, that can be viewed using geographic information systems (GISs). Note that ARD data is only published as symbolic links due to the amount and size of the files. +Logs and analysis-ready-data (ARD) are generated using the [force-l2ps](https://force-eo.readthedocs.io/en/latest/components/lower-level/level2/l2ps.html) command. Logs can be consulted for debugging purposes. ARD may be collected as a basis for other remote sensing workflows. The ARD in `level2_ard/` consist two `.tif` files per initial input image, a quality data file and the atmospherically corrected satellite data. Note that the `.tif` files are only published when the `save_ard` parameter is set to `true` to avoid bloating the memory. ### Higher-level-Processing @@ -84,11 +84,11 @@ Higher level processing consist of two parts, generating parameter files and per Parameter files may be consulted to derive information about the specific processing task performed for a given tile. In this workflow, classification using spectral unmixing is performed. -Spectral unmixing is a common technique to derive sub-pixel classification. Concretely, a set of endmember (provided using `--endmember`) is consulted to determine fractions of different types of vegetation, soil, ... for each pixel. +Spectral unmixing is a common technique to derive sub-pixel classification. Concretely, a set of endmember (provided using `--endmember`) is exploited to determine fractions of different types of vegetation, soil, ... for each pixel. Next, time series analysis for different vegetation characteristics is performed. -The resulting trend files can be consulted to view trends for individual tiles. They are saved as symlinks because of their large size. +The resulting trend files in `trend_files/` can be investigated to view trends for individual tiles. However, these files are only published if the `save_tsa` parameter is set to `true`. If the `return_tss` parameter was set to `true`, the pipeline will also output files with the `TSS` ending. These files contain the time series stack(TSS) for the given tile and index or band. Here, for each date of acquisition, an image is available that contains the values for that date. diff --git a/docs/usage.md b/docs/usage.md index 5413516..2c3c2a7 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -294,6 +294,17 @@ The number of threads can be passed using: The default value is 2. +### Intermediate data publishing + +By default, preprocessing and higher level processing steps do not publish the `.tif` files that they generate to avoid bloating the available memory. However, analysis-ready-data (aka. ARD or level-2 data) and the results of time series analyses (aka. level-3 data) maybe be interesting to certain users. The files for preprocessing and higher level processing can, therefore, be published by setting the `save_ard` and `save_tsa` to `true`, respectively. + +Publishing of intermediate data can be enabled using: + +```bash +save_ard = '[boolean]' +save_tsa = '[boolean]' +``` + ## Running the pipeline The typical command for running the pipeline is as follows: diff --git a/nextflow.config b/nextflow.config index 25fda03..6fc7994 100644 --- a/nextflow.config +++ b/nextflow.config @@ -30,6 +30,8 @@ params { // Workflow configuration group_size = 100 + save_ard = false + save_tsa = false // FORCE force_threads = 2 diff --git a/nextflow_schema.json b/nextflow_schema.json index 0112fb5..b80a19a 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -181,6 +181,18 @@ "description": "Parameters that configure workflow behavior not directly related to FORCE,", "default": "", "properties": { + "save_ard": { + "type": "boolean", + "fa_icon": "fas fa-database", + "description": "Whether analysis ready data should be published to the output directory.", + "help_text": "By default, the results of preprocessing (aka. analyses ready data or level-2 data) are not published. Setting this parameter to `true` enforces that these files are published to the output directory." + }, + "save_tsa": { + "type": "boolean", + "fa_icon": "fas fa-database", + "description": "Whether the results of time series analyses should be published to the output directory.", + "help_text": "By default, the results of higher level processing (aka. level-3 data) are not published. Setting this parameter to `true` enforces that these files are published to the output directory." + }, "group_size": { "type": "integer", "default": 100, From a5df347c9321b6322c8ce18cf135bc0aa96582f0 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Thu, 8 Aug 2024 16:09:18 +0200 Subject: [PATCH 23/61] Added descriptions to custom scripts. --- bin/merge_boa.r | 3 +++ bin/merge_qai.r | 3 +++ bin/test.R | 2 ++ 3 files changed, 8 insertions(+) diff --git a/bin/merge_boa.r b/bin/merge_boa.r index 622ba08..0f4f40b 100755 --- a/bin/merge_boa.r +++ b/bin/merge_boa.r @@ -1,5 +1,8 @@ #!/usr/bin/env Rscript +# Script for merging bottom of atmosphere (boa) .tif raster files. +# This can improve the performance of downstream tasks. + require(raster) args = commandArgs(trailingOnly=TRUE) diff --git a/bin/merge_qai.r b/bin/merge_qai.r index 99f2cff..1ce1f9b 100755 --- a/bin/merge_qai.r +++ b/bin/merge_qai.r @@ -1,5 +1,8 @@ #!/usr/bin/env Rscript +# Script for merging quality information (qai) .tif raster files. +# This can improve the performance of downstream tasks. + require(raster) args = commandArgs(trailingOnly=TRUE) diff --git a/bin/test.R b/bin/test.R index 173cdbb..9ef2f50 100755 --- a/bin/test.R +++ b/bin/test.R @@ -1,5 +1,7 @@ #!/usr/bin/env Rscript +# Script to verify pipeline results from test and test_full profiles. + args = commandArgs(trailingOnly=TRUE) From 2e208fc6441f978e43787f1f6cd51f2279e33fef Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Wed, 21 Aug 2024 15:15:52 +0200 Subject: [PATCH 24/61] Applied more minor changes requested by the reviewers --- docs/output.md | 2 +- docs/usage.md | 12 ++++----- modules/local/check_results.nf | 1 - modules/local/check_results_full.nf | 1 - modules/local/force-generate_analysis_mask.nf | 1 - .../local/force-generate_tile_allow_list.nf | 1 - modules/local/force-higher_level.nf | 3 +-- modules/local/force-mosaic.nf | 3 +-- modules/local/force-preprocess.nf | 3 +-- modules/local/force-pyramid.nf | 3 +-- modules/local/higher_level_force_config.nf | 3 +-- modules/local/merge.nf | 3 +-- modules/local/preprocess_force_config.nf | 3 +-- nextflow_schema.json | 8 +++--- subworkflows/local/higher_level.nf | 3 --- subworkflows/local/preprocessing.nf | 2 +- workflows/rangeland.nf | 26 +++++++++---------- 17 files changed, 32 insertions(+), 46 deletions(-) diff --git a/docs/output.md b/docs/output.md index c32f660..b85caca 100644 --- a/docs/output.md +++ b/docs/output.md @@ -10,7 +10,7 @@ The directories listed below will be created in the results directory after the The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -- [untar](#untar) - Optionally extract input files +- [Untar](#untar) - Optionally extract input files - [Preparation](#preparation) - Create a masks and boundaries for further analyses. - [Preprocessing](#preprocessing) - Preprocessing of satellite imagery. - [Higher-level-Processing](#higher-level-processing) - Classify preprocessed imagery and perform time series analyses. diff --git a/docs/usage.md b/docs/usage.md index 2c3c2a7..e00b793 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -18,8 +18,8 @@ Satellite data should be given as a path to a common root of all imagery. This i ```tree root -├── 181035 -│ └── LE07_L1TP_181035_20061217_20170106_01_T1 +├── 181035 # path/row id +│ └── LE07_L1TP_181035_20061217_20170106_01_T1 # satellite, path/row id, date of acquisition, date of processing, product number, collection tier │ | ├── LE07_L1TP_181035_20061217_20170106_01_T1_ANG.txt │ | ├── LE07_L1TP_181035_20061217_20170106_01_T1_B1.TIF │ | ├── LE07_L1TP_181035_20061217_20170106_01_T1_B2.TIF @@ -34,8 +34,8 @@ root │ | ├── LE07_L1TP_181035_20061217_20170106_01_T1_GCP.txt │ | └── LE07_L1TP_181035_20061217_20170106_01_T1_MTL.txt | └── ... -├── 181036 -│ └── LE07_L1TP_181036_20061217_20170105_01_T1 +├── 181036 # path/row id +│ └── LE07_L1TP_181036_20061217_20170105_01_T1 # satellite, path/row id, date of acquisition, date of processing, product number, collection tier │ | ├── LE07_L1TP_181036_20061217_20170105_01_T1_ANG.txt │ | ├── LE07_L1TP_181036_20061217_20170105_01_T1_B1.TIF │ | ├── LE07_L1TP_181036_20061217_20170105_01_T1_B2.TIF @@ -75,7 +75,7 @@ On the lowest level of the structure, the actual data is stored. Looking at the All files within the lowest level of structure belong to a single observation. Files containing imagery (prefix starts with "B") should be `.tif` files. Files containing auxiliary data are text files. -This structure is automatically generated when [using force to download the data](https://force-eo.readthedocs.io/en/latest/components/lower-level/level1/level1-csd.html?). We strongly suggest users to download data using FORCE. For example, executing the following code (e.g. with [FORCE in docker](https://force-eo.readthedocs.io/en/latest/setup/docker.html)) will download data for Landsat 4,5 and 7, in the time range from 1st January 1984 until 31st December 2006, including pictures with up to 70 percent of cloud coverage: +This structure is automatically generated when [using FORCE to download the data](https://force-eo.readthedocs.io/en/latest/components/lower-level/level1/level1-csd.html?). We strongly suggest users to download data using FORCE. For example, executing the following code (e.g. with [FORCE in docker](https://force-eo.readthedocs.io/en/latest/setup/docker.html)) will download data for Landsat 4,5 and 7, in the time range from 1st January 1984 until 31st December 2006, including pictures with up to 70 percent of cloud coverage: ```bash mkdir -p meta @@ -222,7 +222,7 @@ Note that both parameters are optional and are by default set to: `"LT04,LT05,LE ### Resolution -Resolution of satellite imagery defines the real size of a single pixel. As an example, a resolution of 30 meters indicates that a single pixel in the data covers a 30x30 meters square of the earths surface. Users can customize the resolution that FORCE should assume. This does not necessarily have to match the resolution of the supplied data. FORCE will treat imagery as having the specified resolution. However, passing a resolution not matching the satellite data might lead to unexpected results. Resolution is specified in meters. +Resolution of satellite imagery defines the real size of a single pixel. As an example, a resolution of 30 meters indicates that a single pixel in the data covers a 30x30 meters square of the earth's surface. Users can customize the resolution that FORCE should assume. This does not necessarily have to match the resolution of the supplied data. FORCE will treat imagery as having the specified resolution. However, passing a resolution not matching the satellite data might lead to unexpected results. Resolution is specified in meters. A custom resolution can be passed using: diff --git a/modules/local/check_results.nf b/modules/local/check_results.nf index 28cc76b..4b1e14c 100644 --- a/modules/local/check_results.nf +++ b/modules/local/check_results.nf @@ -1,5 +1,4 @@ process CHECK_RESULTS { - label 'process_low' container 'docker.io/rocker/geospatial:4.3.1' diff --git a/modules/local/check_results_full.nf b/modules/local/check_results_full.nf index f47f6f8..a4c4898 100644 --- a/modules/local/check_results_full.nf +++ b/modules/local/check_results_full.nf @@ -1,5 +1,4 @@ process CHECK_RESULTS_FULL { - label 'process_low' container 'docker.io/rocker/geospatial:4.3.1' diff --git a/modules/local/force-generate_analysis_mask.nf b/modules/local/force-generate_analysis_mask.nf index fa0f711..b9297fe 100644 --- a/modules/local/force-generate_analysis_mask.nf +++ b/modules/local/force-generate_analysis_mask.nf @@ -1,5 +1,4 @@ process FORCE_GENERATE_ANALYSIS_MASK{ - label 'process_single' container "docker.io/davidfrantz/force:3.7.10" diff --git a/modules/local/force-generate_tile_allow_list.nf b/modules/local/force-generate_tile_allow_list.nf index 9f9ad5b..fa1210c 100644 --- a/modules/local/force-generate_tile_allow_list.nf +++ b/modules/local/force-generate_tile_allow_list.nf @@ -1,5 +1,4 @@ process FORCE_GENERATE_TILE_ALLOW_LIST{ - label 'process_single' container "docker.io/davidfrantz/force:3.7.10" diff --git a/modules/local/force-higher_level.nf b/modules/local/force-higher_level.nf index 0300564..9e91773 100644 --- a/modules/local/force-higher_level.nf +++ b/modules/local/force-higher_level.nf @@ -1,9 +1,8 @@ process FORCE_HIGHER_LEVEL { - + tag { tile } label 'process_medium' container "docker.io/davidfrantz/force:3.7.10" - tag { tile } input: tuple val(tile), path(config), path(ard), path(aoi), path (datacube), path (endmember) diff --git a/modules/local/force-mosaic.nf b/modules/local/force-mosaic.nf index aa37395..604df9b 100644 --- a/modules/local/force-mosaic.nf +++ b/modules/local/force-mosaic.nf @@ -1,8 +1,7 @@ process FORCE_MOSAIC{ - + tag { product } label 'process_low' - tag { product } container "docker.io/davidfrantz/force:3.7.10" input: diff --git a/modules/local/force-preprocess.nf b/modules/local/force-preprocess.nf index dc740df..75f5ae3 100644 --- a/modules/local/force-preprocess.nf +++ b/modules/local/force-preprocess.nf @@ -1,7 +1,6 @@ process FORCE_PREPROCESS { - - label 'process_medium' tag { data.simpleName } + label 'process_medium' container "docker.io/davidfrantz/force:3.7.10" diff --git a/modules/local/force-pyramid.nf b/modules/local/force-pyramid.nf index 4eca596..80d430c 100644 --- a/modules/local/force-pyramid.nf +++ b/modules/local/force-pyramid.nf @@ -1,7 +1,6 @@ process FORCE_PYRAMID { - - label 'process_low' tag { tile } + label 'process_low' container "docker.io/davidfrantz/force:3.7.10" diff --git a/modules/local/higher_level_force_config.nf b/modules/local/higher_level_force_config.nf index d22ee7a..afcadf4 100644 --- a/modules/local/higher_level_force_config.nf +++ b/modules/local/higher_level_force_config.nf @@ -1,7 +1,6 @@ process HIGHER_LEVEL_CONFIG { - - label 'process_single' tag { tile } + label 'process_single' container "docker.io/davidfrantz/force:3.7.10" diff --git a/modules/local/merge.nf b/modules/local/merge.nf index 0b8949c..3126637 100644 --- a/modules/local/merge.nf +++ b/modules/local/merge.nf @@ -1,7 +1,6 @@ process MERGE { - - label 'process_low' tag { id } + label 'process_low' container "docker.io/davidfrantz/force:3.7.10" diff --git a/modules/local/preprocess_force_config.nf b/modules/local/preprocess_force_config.nf index 497966a..850675c 100644 --- a/modules/local/preprocess_force_config.nf +++ b/modules/local/preprocess_force_config.nf @@ -1,7 +1,6 @@ process PREPROCESS_CONFIG { - - label 'process_single' tag { data.simpleName } + label 'process_single' container "docker.io/davidfrantz/force:3.7.10" diff --git a/nextflow_schema.json b/nextflow_schema.json index b80a19a..5c0464a 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -16,7 +16,7 @@ "type": "string", "fa_icon": "fas fa-satellite", "help_text": "Set this to the root directory of all satellite imagery. Directory structure should match format of data downloaded with '$ force-level1-csd\n' For concrete directory structure see [usage documentation](https://nf-co.re/rangeland/docs/usage)", - "description": "Root directory of all sattelite imagery.", + "description": "Root directory of all satellite imagery.", "mimetype": "application/x-tar" }, "input_tar": { @@ -41,7 +41,7 @@ "type": "string", "fa_icon": "fas fa-burn", "description": "Water vapor dataset.", - "help_text": "Directory containg a number text files describing global water vapor data at different timestamps, and a coordinate order (`.coo`-)file containig the reference system of the water vapor data." + "help_text": "Directory containing a number text files describing global water vapor data at different timestamps, and a coordinate order (`.coo`-)file containing the reference system of the water vapor data." }, "wvdb_tar": { "type": "boolean", @@ -59,7 +59,7 @@ "type": "string", "fa_icon": "fas fa-chart-area", "description": "Area of interest.", - "help_text": "A single vector file specifying the spacial extend of the targeted area." + "help_text": "A single vector file specifying the spatial extend of the targeted area." }, "endmember": { "type": "string", @@ -196,7 +196,7 @@ "group_size": { "type": "integer", "default": 100, - "description": "Batch size of tiles considered for merging.", + "description": "Batch size of tiles considered for merging.", "help_text": "Controls trade-off between parallelism and I/O load.\nHigher group_size -> More tiles merged in one process, less I/O load.\nLower group_size -> Less tiles merged in one process, more I/O load. ", "fa_icon": "fas fa-layer-group" } diff --git a/subworkflows/local/higher_level.nf b/subworkflows/local/higher_level.nf index fb3bd3b..d0bc922 100644 --- a/subworkflows/local/higher_level.nf +++ b/subworkflows/local/higher_level.nf @@ -22,7 +22,6 @@ workflow HIGHER_LEVEL { FORCE_HIGHER_LEVEL( HIGHER_LEVEL_CONFIG.out.higher_level_configs_and_data ) ch_versions = ch_versions.mix(FORCE_HIGHER_LEVEL.out.versions.first()) - trend_files = FORCE_HIGHER_LEVEL.out.trend_files.flatten().map{ x -> [ x.simpleName.substring(12), x ] } trend_files_mosaic = trend_files.groupTuple() @@ -42,7 +41,5 @@ workflow HIGHER_LEVEL { emit: mosaic_files - versions = ch_versions - } diff --git a/subworkflows/local/preprocessing.nf b/subworkflows/local/preprocessing.nf index 98ce8eb..453c1d8 100644 --- a/subworkflows/local/preprocessing.nf +++ b/subworkflows/local/preprocessing.nf @@ -48,6 +48,7 @@ workflow PREPROCESSING { .toSortedList{ a,b -> a[1][0].simpleName <=> b[1][0].simpleName } .flatMap{it} .groupTuple( remainder : true, size : params.group_size ).map{ [ it[0], it[1] .flatten() ] } + qai_tiles_to_merge = qai_tiles.filter{ x -> x[1].size() > 1 } .map{ [ it[0].substring( 0, 11 ), it[1] ] } //Sort to ensure the same groups if you use resume @@ -76,5 +77,4 @@ workflow PREPROCESSING { emit: tiles_and_masks = boa_tiles.join( qai_tiles ).join( masks ) versions = ch_versions - } diff --git a/workflows/rangeland.nf b/workflows/rangeland.nf index 08cda4d..dd01dfd 100644 --- a/workflows/rangeland.nf +++ b/workflows/rangeland.nf @@ -46,7 +46,7 @@ include { UNTAR as UNTAR_INPUT; UNTAR as UNTAR_DEM; UNTAR as UNTAR_WVDB; UNTAR a */ -// check wether provided input is within provided time range +// check whether provided input is within provided time range def inRegion = input -> { Integer date = input.simpleName.split("_")[3] as Integer Integer start = params.start_date.replace('-','') as Integer @@ -75,9 +75,9 @@ workflow RANGELAND { data = null dem = null wvdb = null - cube_file = file( "$params.data_cube" ) - aoi_file = file( "$params.aoi" ) - endmember_file = file( "$params.endmember" ) + cube_file = file( params.data_cube ) + aoi_file = file( params.aoi ) + endmember_file = file( params.endmember ) // // MODULE: untar @@ -88,12 +88,12 @@ workflow RANGELAND { base_path = UNTAR_INPUT.out.untar.map(it -> it[1]) data = base_path.map(it -> file("$it/*/*", type: 'dir')).flatten() - data = data.flatten().filter{ inRegion(it) } + data = data.filter{ inRegion(it) } tar_versions = tar_versions.mix(UNTAR_INPUT.out.versions) } else { - data = Channel.fromPath( "${params.input}/*/*", type: 'dir') .flatten() - data = data.flatten().filter{ inRegion(it) } + data = Channel.fromPath( "${params.input}/*/*", type: 'dir').flatten() + data = data.filter{ inRegion(it) } } if (params.dem_tar) { @@ -133,12 +133,12 @@ workflow RANGELAND { // MODULE: Check results // if (params.config_profile_name == 'Test profile') { - woody_change_ref = file("$params.woody_change_ref") - woody_yoc_ref = file("$params.woody_yoc_ref") - herbaceous_change_ref = file("$params.herbaceous_change_ref") - herbaceous_yoc_ref = file("$params.herbaceous_yoc_ref") - peak_change_ref = file("$params.peak_change_ref") - peak_yoc_ref = file("$params.peak_yoc_ref") + woody_change_ref = file( params.woody_change_ref ) + woody_yoc_ref = file( params.woody_yoc_ref ) + herbaceous_change_ref = file( params.herbaceous_change_ref ) + herbaceous_yoc_ref = file( params.herbaceous_yoc_ref ) + peak_change_ref = file( params.peak_change_ref ) + peak_yoc_ref = file( params.peak_yoc_ref ) CHECK_RESULTS(grouped_trend_data, woody_change_ref, woody_yoc_ref, herbaceous_change_ref, herbaceous_yoc_ref, peak_change_ref, peak_yoc_ref) ch_versions = ch_versions.mix(CHECK_RESULTS.out.versions) From e5d44259781d7382a48e0f6ea74e20cf57943134 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Fri, 23 Aug 2024 09:29:22 +0200 Subject: [PATCH 25/61] Reworked merge scripts --- bin/merge_boa.r | 61 +++++++++++++++++++++++-------------------------- bin/merge_qai.r | 50 +++++++++++++++++++--------------------- 2 files changed, 53 insertions(+), 58 deletions(-) diff --git a/bin/merge_boa.r b/bin/merge_boa.r index 0f4f40b..7a488b4 100755 --- a/bin/merge_boa.r +++ b/bin/merge_boa.r @@ -3,44 +3,41 @@ # Script for merging bottom of atmosphere (boa) .tif raster files. # This can improve the performance of downstream tasks. -require(raster) +require(terra) -args = commandArgs(trailingOnly=TRUE) +args <- commandArgs(trailingOnly = TRUE) if (length(args) < 3) { - stop("\nthis program needs at least 3 inputs\n1: output filename\n2-*: input files", call.=FALSE) + stop("\nError: this program needs at least 3 inputs\n1: output filename\n2-*: input files", call.=FALSE) } fout <- args[1] finp <- args[2:length(args)] -nf <- length(finp) -img <- brick(finp[1]) -nc <- ncell(img) -nb <- nbands(img) - - -sum <- matrix(0, nc, nb) -num <- matrix(0, nc, nb) - -for (i in 1:nf){ - - data <- brick(finp[i])[] - - num <- num + !is.na(data) - - data[is.na(data)] <- 0 - sum <- sum + data - -} - -mean <- sum/num -img[] <- mean - - -writeRaster(img, filename = fout, format = "GTiff", datatype = "INT2S", - options = c("INTERLEAVE=BAND", "COMPRESS=LZW", "PREDICTOR=2", - "NUM_THREADS=ALL_CPUS", "BIGTIFF=YES", - sprintf("BLOCKXSIZE=%s", img@file@blockcols[1]), - sprintf("BLOCKYSIZE=%s", img@file@blockrows[1]))) +# Load input rasters +rasters <- lapply(finp, rast) + +# Calculate the sum of non-NA values across all rasters +sum_rasters <- Reduce("+", lapply(rasters, function(x) { + x[is.na(x)] <- 0 + return(x) +})) + +# Calculate the number of values non-NA values for each cell +count_rasters <- Reduce("+", lapply(rasters, function(x) { + return(!is.na(x)) +})) + +# Calculate the mean raster +mean_raster <- sum_rasters / count_rasters + +# Write the mean raster +writeRaster(mean_raster, + filename = fout, + datatype = "INT2S", + filetype = "GTiff", + gdal = c("COMPRESS=LZW", "PREDICTOR=2", + "NUM_THREADS=ALL_CPUS", "BIGTIFF=YES", + sprintf("BLOCKXSIZE=%s", ncol(mean_raster)), + sprintf("BLOCKYSIZE=%s", nrow(mean_raster)))) diff --git a/bin/merge_qai.r b/bin/merge_qai.r index 1ce1f9b..a35131a 100755 --- a/bin/merge_qai.r +++ b/bin/merge_qai.r @@ -3,38 +3,36 @@ # Script for merging quality information (qai) .tif raster files. # This can improve the performance of downstream tasks. -require(raster) +require(terra) -args = commandArgs(trailingOnly=TRUE) +args <- commandArgs(trailingOnly = TRUE) if (length(args) < 3) { - stop("\nthis program needs at least 3 inputs\n1: output filename\n2-*: input files", call.=FALSE) + stop("\nError: this program needs at least 3 inputs\n1: output filename\n2-*: input files", call.=FALSE) } fout <- args[1] finp <- args[2:length(args)] -nf <- length(finp) -img <- raster(finp[1]) -nc <- ncell(img) - - -last <- rep(1, nc) - -for (i in 1:nf){ - - data <- raster(finp[i])[] - - last[!is.na(data)] <- data[!is.na(data)] - -} - -img[] <- last - - -writeRaster(img, filename = fout, format = "GTiff", datatype = "INT2S", - options = c("INTERLEAVE=BAND", "COMPRESS=LZW", "PREDICTOR=2", - "NUM_THREADS=ALL_CPUS", "BIGTIFF=YES", - sprintf("BLOCKXSIZE=%s", img@file@blockcols[1]), - sprintf("BLOCKYSIZE=%s", img@file@blockrows[1]))) +# load raster files into single SpatRaster +rasters <- rast(finp) + +# Merge rasters by maintaining the last non-NA value +merged_raster <- app(rasters, function(x) { + non_na_values <- na.omit(x) + if (length(non_na_values) == 0) { + return(1) + } + return(tail(non_na_values, 1)[1]) +}) + +# Write merged raster +writeRaster(merged_raster, + filename = fout, + filetype = "GTiff", + datatype = "INT2S", + gdal = c("INTERLEAVE=BAND", "COMPRESS=LZW", "PREDICTOR=2", + "NUM_THREADS=ALL_CPUS", "BIGTIFF=YES", + sprintf("BLOCKXSIZE=%s", ncol(merged_raster)), + sprintf("BLOCKYSIZE=%s", nrow(merged_raster)))) From fd29a4bf1a0ebd8a5c70865e67cd8c108dedef59 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Fri, 23 Aug 2024 09:49:34 +0200 Subject: [PATCH 26/61] Added authors and licenses to scripts --- bin/merge_boa.r | 3 +++ bin/merge_qai.r | 3 +++ bin/test.R | 3 +++ 3 files changed, 9 insertions(+) diff --git a/bin/merge_boa.r b/bin/merge_boa.r index 7a488b4..318164f 100755 --- a/bin/merge_boa.r +++ b/bin/merge_boa.r @@ -1,5 +1,8 @@ #!/usr/bin/env Rscript +## Originally written by Felix Kummer and released under the MIT license. +## See git repository (https://github.com/nf-core/rangeland) for full license text. + # Script for merging bottom of atmosphere (boa) .tif raster files. # This can improve the performance of downstream tasks. diff --git a/bin/merge_qai.r b/bin/merge_qai.r index a35131a..8515063 100755 --- a/bin/merge_qai.r +++ b/bin/merge_qai.r @@ -1,5 +1,8 @@ #!/usr/bin/env Rscript +## Originally written by Felix Kummer and released under the MIT license. +## See git repository (https://github.com/nf-core/rangeland) for full license text. + # Script for merging quality information (qai) .tif raster files. # This can improve the performance of downstream tasks. diff --git a/bin/test.R b/bin/test.R index 9ef2f50..298b023 100755 --- a/bin/test.R +++ b/bin/test.R @@ -1,5 +1,8 @@ #!/usr/bin/env Rscript +## Originally written by David Frantz and Felix Kummer and released under the MIT license. +## See git repository (https://github.com/nf-core/rangeland) for full license text. + # Script to verify pipeline results from test and test_full profiles. args = commandArgs(trailingOnly=TRUE) From e042a1c9d9bbac111d5603f99874898ba2d854ce Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Fri, 23 Aug 2024 10:06:31 +0200 Subject: [PATCH 27/61] Removed unused sensors_level1 parameter --- conf/test.config | 1 - conf/test_full.config | 1 - docs/usage.md | 20 ++++---------------- nextflow.config | 1 - nextflow_schema.json | 7 ------- 5 files changed, 4 insertions(+), 26 deletions(-) diff --git a/conf/test.config b/conf/test.config index 6b6e679..8b5506f 100644 --- a/conf/test.config +++ b/conf/test.config @@ -36,7 +36,6 @@ params { start_date = '1987-01-01' end_date = '1989-12-31' - sensors_level1 = 'LT04,LT05' sensors_level2 = 'LND04 LND05' // Reference data diff --git a/conf/test_full.config b/conf/test_full.config index 907fdf8..b963c70 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -32,7 +32,6 @@ params { start_date = '1986-01-01' end_date = '1989-12-31' - sensors_level1 = 'LT04,LT05' sensors_level2 = 'LND04 LND05' // enable time series stack output diff --git a/docs/usage.md b/docs/usage.md index e00b793..99f6bd1 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -189,18 +189,7 @@ Users can specify additional parameters to configure how the underlying workflow ### Sensor Levels -Data from different satellites can be processed within this workflow. Users may wish to include different satellites in preprocessing and in higher level processing. To control this behavior, two parameters can be set when the pipeline is launched. -The first parameter - `sensors_level1` - controls the selection of satellites for preprocessing. This parameter should follow the FORCE notation for level 1 processing of satellites. Concretely, a string containing comma-separated satellite identifiers has to be supplied (e.g. `"LT04,LT05"` to include Landsat 4 and 5). Available options for satellite identifiers are: - -- `"LT04"`: Landsat 4 TM -- `"LT05"`: Landsat 5 TM -- `"LE07"`: Landsat 7 ETM+ -- `"LC08"`: Landsat 8 OLI -- `"S2A"`: Sentinel-2A MSI -- `"S2B"`: Sentinel-2B MSI - -The second parameter - `sensors_level2` - controls the selection of satellites for the higher level processing steps. The parameter has to follow the FORCE notation for level 2 processing. In particular, a string containing space-separated satellite identifiers has to be supplied (e.g. `"LND04 LND05"` to include Landsat 4 and 5). Note that these identifiers differ from those used for the `sensors_level1` parameter. -More details on available satellite identifiers can be found [here](https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html), some common options include: +Data from different satellites can be processed within this workflow. Users may wish to include different satellites in preprocessing and in higher level processing. All input imagery is preprocessed. The `sensors_level2` parameter controls the selection of satellites for the higher level processing steps. The parameter has to follow the FORCE notation for level 2 processing. In particular, a string containing space-separated satellite identifiers has to be supplied (e.g. `"LND04 LND05"` to include Landsat 4 and 5). More details on available satellite identifiers can be found [here](https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html), some common options include: - `"LND04"`: 6-band Landsat 4 TM - `"LND05"`: 6-band Landsat 5 TM @@ -209,16 +198,15 @@ More details on available satellite identifiers can be found [here](https://forc - `"SEN2A"`: 10-band Sentinel-2A - `"SEN2B"`: 10-band Sentinel-2B -Note that the identifiers specified for both processing levels have to match the data made available to the workflow. In other words, satellite data for e.g. Landsat 5 can't be processed if it was not supplied using the `input` parameter. +Note that the specified identifiers have to match the data made available to the workflow. In other words, satellite data for e.g. Landsat 5 can't be processed if it was not supplied using the `input` parameter. -Both parameters can be passed as follows: +The satellite identifiers can be passed as follows: ```bash ---sensors_level1 = '[preprocessing satellite identifier string]' --sensors_level2 = '[higher level processing satellite identifier string]' ``` -Note that both parameters are optional and are by default set to: `"LT04,LT05,LE07,S2A"` and `"LND04 LND05 LND07"`. Therefore, by default, the pipeline will use Landsat 4,5,7, and Sentinel 2 for preprocessing, while using Landsat 4,5 and 7 for higher level processing. +Note that the parameter is optional and the default value is: `"LND04 LND05 LND07"`. Therefore, by default, the pipeline will use Landsat 4,5 and 7 imagery in higher level processing. ### Resolution diff --git a/nextflow.config b/nextflow.config index 6fc7994..2aacdca 100644 --- a/nextflow.config +++ b/nextflow.config @@ -22,7 +22,6 @@ params { wvdb_tar = false // Remote sensing imagery parameters - sensors_level1 = "LT04,LT05,LE07,S2A" sensors_level2 = "LND04 LND05 LND07" resolution = 30 start_date = "1984-01-01" diff --git a/nextflow_schema.json b/nextflow_schema.json index 5c0464a..4fb0b6d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -94,13 +94,6 @@ "default": "", "help_text": "These parameters are used to tell pipeline tools which data is expected, how this data has to be incorporated, and which parts of the imagery can be excluded (e.g. due to temporal mismatch). These parameters should be closely aligned with the provided input data.", "properties": { - "sensors_level1": { - "type": "string", - "default": "LT04,LT05,LE07,S2A", - "fa_icon": "fas fa-satellite", - "description": "Satellites for which first level data should be processed.", - "help_text": "String containing comma-separated indicators of satellites in FORCE level 1format. Possible options: \n\"LT04\": Landsat 4 TM, \n\"LT05\": Landsat 5 TM, \n\"LE07\": Landsat 7 ETM+,\n\"LC08\": Landsat 8 OLI,\n\"S2A\": Sentinel-2A MSI,\n\"S2B\": Sentinel-2B MSI" - }, "sensors_level2": { "type": "string", "default": "LND04 LND05 LND07", From 69fd4937ac94fd990383642bea430bcdc37c5c6c Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Wed, 28 Aug 2024 16:57:41 +0200 Subject: [PATCH 28/61] Applied one-sentence-per-line-scheme in .md files --- README.md | 19 +++-- docs/output.md | 70 ++++++++++++++----- docs/usage.md | 184 ++++++++++++++++++++++++++++++++++++------------- 3 files changed, 201 insertions(+), 72 deletions(-) diff --git a/README.md b/README.md index a95323f..8630c00 100644 --- a/README.md +++ b/README.md @@ -19,7 +19,8 @@ ## Introduction -**nf-core/rangeland** is a geographical best-practice analysis pipeline for remotely sensed imagery. The pipeline processes satellite imagery alongside auxiliary data in multiple steps to arrive at a set of trend files related to land-cover changes. The main pipeline steps are: +**nf-core/rangeland** is a geographical best-practice analysis pipeline for remotely sensed imagery. +The pipeline processes satellite imagery alongside auxiliary data in multiple steps to arrive at a set of trend files related to land-cover changes. The main pipeline steps are: 1. Read satellite imagery, digital elevation model, endmember definition, water vapor database and area of interest definition 2. Generate allow list and analysis mask to determine which pixels from the satellite data can be used @@ -33,9 +34,12 @@ ## Usage > [!NOTE] -> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. +> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. +> Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. -To run the pipeline on real data, input data needs to be acquired. Concretely, satellite imagery, water vapor data, a digital elevation model, endmember definitions, a datacube specification, and a area-of-interest specification are required. Please refer to the [usage documentation](https://nf-co.re/rangeland/usage) for details on the input structure. +To run the pipeline on real data, input data needs to be acquired. +Concretely, satellite imagery, water vapor data, a digital elevation model, endmember definitions, a datacube specification, and a area-of-interest specification are required. +Please refer to the [usage documentation](https://nf-co.re/rangeland/usage) for details on the input structure. Now, you can run the pipeline using: @@ -52,7 +56,8 @@ nextflow run nf-core/rangeland \ ``` > [!WARNING] -> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; +> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. +> Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; > see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rangeland/usage) and the [parameter documentation](https://nf-co.re/rangeland/parameters). @@ -72,7 +77,8 @@ The rangeland workflow was originally written by: The original workflow can be found on [github](https://github.com/CRC-FONDA/FORCE2NXF-Rangeland). -Transformation to nf-core/rangeland was conducted by [Felix Kummer](https://github.com/Felix-Kummer). nf-core alignment started on the [nf-core branch of the original repository](https://github.com/CRC-FONDA/FORCE2NXF-Rangeland/tree/nf-core). +Transformation to nf-core/rangeland was conducted by [Felix Kummer](https://github.com/Felix-Kummer). +nf-core alignment started on the [nf-core branch of the original repository](https://github.com/CRC-FONDA/FORCE2NXF-Rangeland/tree/nf-core). We thank the following people for their extensive assistance in the development of this pipeline: @@ -114,7 +120,8 @@ You can cite the `nf-core` publication as follows: > > _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x). -This pipeline is based one the publication listed below. The publication can be cited as follows: +This pipeline is based one the publication listed below. +The publication can be cited as follows: > **FORCE on Nextflow: Scalable Analysis of Earth Observation Data on Commodity Clusters** > diff --git a/docs/output.md b/docs/output.md index b85caca..b13b1d0 100644 --- a/docs/output.md +++ b/docs/output.md @@ -4,7 +4,8 @@ This document describes the output produced by the pipeline. -The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. +The directories listed below will be created in the results directory after the pipeline has finished. +All paths are relative to the top-level results directory. ## Pipeline overview @@ -24,15 +25,22 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d Output files - `untar/` - - ``: directory containing symlinks to decompressed digital elevation input data. Only present if a tar archive was provided for the digital elevation model. Name of the directory derived from archive contents. - - ``: directory containing symlinks to decompressed water vapor input data. Only present if a tar archive was provided for water vapor data. Name of the directory derived from archive contents. - - ``: directory containing symlinks to decompressed satellite imagery input data. Only present if a tar archive was provided for satellite data. Name of the directory derived from archive contents. + - ``: directory containing symlinks to decompressed digital elevation input data. + Only present if a tar archive was provided for the digital elevation model. + Name of the directory derived from archive contents. + - ``: directory containing symlinks to decompressed water vapor input data. + Only present if a tar archive was provided for water vapor data. + Name of the directory derived from archive contents. + - ``: directory containing symlinks to decompressed satellite imagery input data. + Only present if a tar archive was provided for satellite data. + Name of the directory derived from archive contents. [untar](https://nf-co.re/modules/untar) is a nf-core module used to extract files from tar archives. -Invokation of untar depends on certain parameters (i.e `input_tar`, `dem_tar` and `wvdb_tar`). Thus, the outputs files are only generated when these are set to `true`. +Invokation of untar depends on certain parameters (i.e `input_tar`, `dem_tar` and `wvdb_tar`). +Thus, the outputs files are only generated when these are set to `true`. ### Preparation @@ -40,16 +48,23 @@ Invokation of untar depends on certain parameters (i.e `input_tar`, `dem_tar` an Output files - `preparation/` - - `tile_allow.txt`: File containing all [FORCE](https://force-eo.readthedocs.io/en/latest/index.html) notation tiles of the earths surface that should be used further in the pipeline. The first line contains the number of tiles. Following lines contain tile identifiers. - - `mask/`: Directory containing a subdirectory for every [FORCE](https://force-eo.readthedocs.io/en/latest/index.html) tile. Each subdirectory contains the `aoi.tif` file. This file represents a binary mask layer that indicates which pixels are eligible for analyses. + - `tile_allow.txt`: File containing all [FORCE](https://force-eo.readthedocs.io/en/latest/index.html) notation tiles of the earths surface that should be used further in the pipeline. + The first line contains the number of tiles. + Following lines contain tile identifiers. + - `mask/`: Directory containing a subdirectory for every [FORCE](https://force-eo.readthedocs.io/en/latest/index.html) tile. + Each subdirectory contains the `aoi.tif` file. + This file represents a binary mask layer that indicates which pixels are eligible for analyses. In the preparation step, usable tiles and pixels per tile are identified. -[force-tile-extent](https://force-eo.readthedocs.io/en/latest/components/auxilliary/tile-extent.html#force-tile-extent) analyses the area of interest information and determines the tiles that can be used. These tiles are later used by other [FORCE](https://force-eo.readthedocs.io/en/latest/index.html) submodules. +[force-tile-extent](https://force-eo.readthedocs.io/en/latest/components/auxilliary/tile-extent.html#force-tile-extent) analyses the area of interest information and determines the tiles that can be used. +These tiles are later used by other [FORCE](https://force-eo.readthedocs.io/en/latest/index.html) submodules. -[force-cube](https://force-eo.readthedocs.io/en/latest/components/auxilliary/cube.html#force-cube) computes the usable pixels for each [FORCE](https://force-eo.readthedocs.io/en/latest/index.html) tile. This computation is based on the specified are of interest and the resolution. The resulting binary masks can be used to understand which pixels were discarded (e.g. because they only contain water). +[force-cube](https://force-eo.readthedocs.io/en/latest/components/auxilliary/cube.html#force-cube) computes the usable pixels for each [FORCE](https://force-eo.readthedocs.io/en/latest/index.html) tile. +This computation is based on the specified are of interest and the resolution. +The resulting binary masks can be used to understand which pixels were discarded (e.g. because they only contain water). ### Preprocessing @@ -58,7 +73,8 @@ In the preparation step, usable tiles and pixels per tile are identified. - `preprocess//` - `param_files/`: Directory containing parameter files for [FORCE](https://force-eo.readthedocs.io/en/latest/index.html) preprocessing modules. One file per satellite mission per tile. - - `level2_ard/`: Directory containing symlinks to analysis-ready-data. Subdirectories contain the .tif files that were generated during preprocessing. + - `level2_ard/`: Directory containing symlinks to analysis-ready-data. + Subdirectories contain the .tif files that were generated during preprocessing. - `logs/`: Logs from preprocessing. @@ -67,7 +83,11 @@ Preprocessing consist of two parts, generating parameter files and actual prepro The parameter files created through [force-parameter](https://force-eo.readthedocs.io/en/latest/components/auxilliary/parameter.html#force-parameter) can be viewed to understand concrete preprocessing techniques applied for a given tile. -Logs and analysis-ready-data (ARD) are generated using the [force-l2ps](https://force-eo.readthedocs.io/en/latest/components/lower-level/level2/l2ps.html) command. Logs can be consulted for debugging purposes. ARD may be collected as a basis for other remote sensing workflows. The ARD in `level2_ard/` consist two `.tif` files per initial input image, a quality data file and the atmospherically corrected satellite data. Note that the `.tif` files are only published when the `save_ard` parameter is set to `true` to avoid bloating the memory. +Logs and analysis-ready-data (ARD) are generated using the [force-l2ps](https://force-eo.readthedocs.io/en/latest/components/lower-level/level2/l2ps.html) command. +Logs can be consulted for debugging purposes. +ARD may be collected as a basis for other remote sensing workflows. +The ARD in `level2_ard/` consist two `.tif` files per initial input image, a quality data file and the atmospherically corrected satellite data. +Note that the `.tif` files are only published when the `save_ard` parameter is set to `true` to avoid bloating the storage. ### Higher-level-Processing @@ -82,15 +102,20 @@ Logs and analysis-ready-data (ARD) are generated using the [force-l2ps](https:// Higher level processing consist of two parts, generating parameter files and performing various processing task as defined in the parameter files. -Parameter files may be consulted to derive information about the specific processing task performed for a given tile. In this workflow, classification using spectral unmixing is performed. +Parameter files may be consulted to derive information about the specific processing task performed for a given tile. +In this workflow, classification using spectral unmixing is performed. -Spectral unmixing is a common technique to derive sub-pixel classification. Concretely, a set of endmember (provided using `--endmember`) is exploited to determine fractions of different types of vegetation, soil, ... for each pixel. +Spectral unmixing is a common technique to derive sub-pixel classification. +Concretely, a set of endmember (provided using `--endmember`) is exploited to determine fractions of different types of vegetation, soil, ... for each pixel. Next, time series analysis for different vegetation characteristics is performed. -The resulting trend files in `trend_files/` can be investigated to view trends for individual tiles. However, these files are only published if the `save_tsa` parameter is set to `true`. +The resulting trend files in `trend_files/` can be investigated to view trends for individual tiles. +However, these files are only published if the `save_tsa` parameter is set to `true`. -If the `return_tss` parameter was set to `true`, the pipeline will also output files with the `TSS` ending. These files contain the time series stack(TSS) for the given tile and index or band. Here, for each date of acquisition, an image is available that contains the values for that date. +If the `return_tss` parameter was set to `true`, the pipeline will also output files with the `TSS` ending. +These files contain the time series stack(TSS) for the given tile and index or band. +Here, for each date of acquisition, an image is available that contains the values for that date. ### Visualization @@ -105,7 +130,11 @@ If the `return_tss` parameter was set to `true`, the pipeline will also output f -Two types of common visualizations are generated in the last step of the pipeline. They are results of [force-mosaic](https://force-eo.readthedocs.io/en/latest/components/auxilliary/mosaic.html) and [force-pyramid](https://force-eo.readthedocs.io/en/latest/components/auxilliary/pyramid.html). Note that these visualizations do not add more logic to the workflow but rather rearrange the output files of higher-level-processing. Both visualizations are enabled by default but may be disabled in a certain configuration files. Thus, these outputs are optional. +Two types of common visualizations are generated in the last step of the pipeline. +They are results of [force-mosaic](https://force-eo.readthedocs.io/en/latest/components/auxilliary/mosaic.html) and [force-pyramid](https://force-eo.readthedocs.io/en/latest/components/auxilliary/pyramid.html). +Note that these visualizations do not add more logic to the workflow but rather rearrange the output files of higher-level-processing. +Both visualizations are enabled by default but may be disabled in a certain configuration files. +Thus, these outputs are optional. ### MultiQC @@ -119,9 +148,11 @@ Two types of common visualizations are generated in the last step of the pipelin -[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. +[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. +Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. -Results generated by MultiQC collate pipeline QC from supported tools. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . +Results generated by MultiQC collate pipeline QC from supported tools. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. +For more information about how to use MultiQC reports, see . ### Pipeline information @@ -136,4 +167,5 @@ Results generated by MultiQC collate pipeline QC from supported tools. The pipel -[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. +[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. +This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. diff --git a/docs/usage.md b/docs/usage.md index 99f6bd1..074b9ae 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -6,15 +6,20 @@ ## Input -As most remote sensing workflows, this pipeline relies on numerous sources of data. In the following we will describe the required data and corresponding formats. Mandatory input data consists of satellite data, a digital elevation model, a water vapor database, a data_cube, an area-of-interest specification and an endmember definition. +As most remote sensing workflows, this pipeline relies on numerous sources of data. +In the following we will describe the required data and corresponding formats. +Mandatory input data consists of satellite data, a digital elevation model, a water vapor database, a data_cube, an area-of-interest specification and an endmember definition. ### Satellite data -This pipeline operates on Landsat data. Landsat is a joint NASA/U.S. Geolical Survey satellite mission that provides continuous Earth obersvation data since 1984 at 30m spatial resolution with a temporal revisit frequency of 8-16 days. -Landsast carries multispectral optical instruments that observe the land surface in the visible to shortwave infrared spectrum. -For infos on Landsat, see [here](https://www.usgs.gov/core-science-systems/nli/landsat). +This pipeline operates on Landsat data. +Landsat is a joint NASA/U.S. Geolical Survey satellite mission that provides continuous Earth obersvation data since 1984 at 30m spatial resolution with a temporal revisit frequency of 8-16 days. +Landsat satellites carry multispectral optical instruments that observe the land surface in the visible to shortwave infrared spectrum. +For information on Landsat, see [here](https://www.usgs.gov/core-science-systems/nli/landsat). -Satellite data should be given as a path to a common root of all imagery. This is a common format used in geographic information systems, including FORCE, which is applied in this pipeline. The expected structure underneath the root directory should follow this example: +Satellite data should be given as a path to a common root of all imagery. +This is a common format used in geographic information systems, including FORCE, which is applied in this pipeline. +The expected structure underneath the root directory should follow this example: ```tree root @@ -53,9 +58,11 @@ root └── ... ``` -Subdirectories of `root/` contain _path_ and _row_ information as commonly used for Landsat imagery. As an example, the sub directory `181036/` contains imagery for path 18 and row 1036. +Subdirectories of `root/` contain _path_ and _row_ information as commonly used for Landsat imagery. +For example, the sub directory `181036/` contains imagery for path 18 and row 1036. -The next level of subdirectories contains the data for a specific day and from a specific source. Lets look at the example `LE07_L1TP_181036_20061217_20170105_01_T1`: +The next level of subdirectories contains the data for a specific day and from a specific source. +Lets look at the example `LE07_L1TP_181036_20061217_20170105_01_T1`: - "LE07" corresponds to Landsat 7 Enhanced - "L1TP" corresponds to Level-1 Terrain Corrected imagery @@ -65,7 +72,9 @@ The next level of subdirectories contains the data for a specific day and from a - "01" corresponds to version number of the remote sensing product - "T1" corresponds to the Tier of the data collection, which indicates the Tier 1 landsat collection in this case -On the lowest level of the structure, the actual data is stored. Looking at the contents of `LE07_L1TP_181036_20061217_20170105_01_T1`, we see that all files share the same prefix, followed by a specification of the specific files contents. These suffixes include: +On the lowest level of the structure, the actual data is stored. +Looking at the contents of `LE07_L1TP_181036_20061217_20170105_01_T1`, we see that all files share the same prefix, followed by a specification of the specific files contents. +These suffixes include: - "B" followed by a number _i_ identifying the band of the satellite (band 6 has two files as Landsat 7 has two thermal bands) - "BQA" identifies the quality information band @@ -73,9 +82,13 @@ On the lowest level of the structure, the actual data is stored. Looking at the - "ANG" identifies angle of observation and other geometric information information - "MTL" identifies meta data -All files within the lowest level of structure belong to a single observation. Files containing imagery (prefix starts with "B") should be `.tif` files. Files containing auxiliary data are text files. +All files within the lowest level of structure belong to a single observation. +Files containing imagery (prefix starts with "B") should be `.tif` files. +Files containing auxiliary data are text files. -This structure is automatically generated when [using FORCE to download the data](https://force-eo.readthedocs.io/en/latest/components/lower-level/level1/level1-csd.html?). We strongly suggest users to download data using FORCE. For example, executing the following code (e.g. with [FORCE in docker](https://force-eo.readthedocs.io/en/latest/setup/docker.html)) will download data for Landsat 4,5 and 7, in the time range from 1st January 1984 until 31st December 2006, including pictures with up to 70 percent of cloud coverage: +This structure is automatically generated when [using FORCE to download the data](https://force-eo.readthedocs.io/en/latest/components/lower-level/level1/level1-csd.html?). +We strongly suggest users to download data using FORCE. +For example, executing the following code (e.g. with [FORCE in docker](https://force-eo.readthedocs.io/en/latest/setup/docker.html)) will download data for Landsat 4,5 and 7, in the time range from 1st January 1984 until 31st December 2006, including pictures with up to 70 percent of cloud coverage: ```bash mkdir -p meta @@ -84,7 +97,8 @@ mkdir -p data force-level1-csd -s "LT04,LT05,LE07" -d "19840101,20061231" -c 0,70 meta/ data/ queue.txt vector/aoi.gpkg ``` -Note that you need to pass an area-of-interest file, see the area of interest section [Area of interest](#area-of-interest-aoi) for details. +Note that an area-of-interest file has to be passed, see the area of interest section [Area of interest](#area-of-interest-aoi) for details. +In addition, downloading data using FORCE may require access the machine-to-machine interfaces. The satellite imagery can be given to the pipeline using: @@ -92,13 +106,18 @@ The satellite imagery can be given to the pipeline using: --input '[path to imagery root]' ``` -The satellite imagery can also be provide as a tar archive. In this case it is mandatory to set `--input_tar` to `true`. Moreover, within the tar archive, the structure explained above has to be in place. In the example above `181036/` and `181035/` would need to be in the top level of the archive. +The satellite imagery can also be provide as a tar archive. +In this case it is mandatory to set `--input_tar` to `true`. +Moreover, within the tar archive, the structure explained above has to be in place. +In the example above `181036/` and `181035/` would need to be in the top level of the archive. ### Digital Elevation Model (DEM) -A DEM is necessary for topographic correction of Landsat data, and helps to distinguish between cloud, shadows and water surfaces. Common sources for digital elevation models are [Copernicus](https://www.copernicus.eu/en),[Shuttle Radar Topography Mission](https://www2.jpl.nasa.gov/srtm/) (SRTM), or [Advanced Spaceborne Thermal Emission and Reflection Radiometer](https://asterweb.jpl.nasa.gov/) (ASTER). +A DEM is necessary for topographic correction of Landsat data, and helps to distinguish between cloud, shadows and water surfaces. +Common sources for digital elevation models are [Copernicus](https://www.copernicus.eu/en),[Shuttle Radar Topography Mission](https://www2.jpl.nasa.gov/srtm/) (SRTM), or [Advanced Spaceborne Thermal Emission and Reflection Radiometer](https://asterweb.jpl.nasa.gov/) (ASTER). -The pipeline expects a path to the Digital Elevation Model root directory as a parameter. Concretely, the expected structure would look like this: +The pipeline expects a path to the Digital Elevation Model root directory as a parameter. +Concretely, the expected structure would look like this: ```tree dem @@ -115,7 +134,10 @@ The DEM can be given to the pipeline using: --dem '[path to dem root]' ``` -The digital elevation model can also be provide as a tar archive. In this case it is mandatory to set `--dem_tar` to `true`. Moreover, within the tar archive, the structure explained above has to be in place. In the example above `.vrt` and `/` would need to be in the top level of the archive. +The digital elevation model can also be provide as a tar archive. +In this case it is mandatory to set `--dem_tar` to `true`. +Moreover, within the tar archive, the structure explained above has to be in place. +In the example above `.vrt` and `/` would need to be in the top level of the archive. ### Water Vapor Database (WVDB) @@ -138,11 +160,14 @@ The WVDB can be given to the pipeline using: --wvdb '[path to wvdb dir]' ``` -The water vapor database can also be provide as a tar archive. In this case it is mandatory to set `--wvdb_tar` to `true`. All files of the wvdb would need to be in the top level of the archive. +The water vapor database can also be provide as a tar archive. +In this case it is mandatory to set `--wvdb_tar` to `true`. +All files of the wvdb would need to be in the top level of the archive. ### Datacube -The datacube definition stores information about the projection and reference grid of the generated datacube. For details see the [FORCE main paper](https://www.mdpi.com/2072-4292/11/9/1124). +The datacube definition stores information about the projection and reference grid of the generated datacube. +For details see the [FORCE main paper](https://www.mdpi.com/2072-4292/11/9/1124). The datacube definition is passed as a single file using: @@ -175,7 +200,8 @@ An example endmember definition (developed in [Hostert et al. 2003](https://www. 710 3220 5490 0 ``` -Each colum represents a different endmember. Columns represent Landsat bands (R,G,B, NIR, SWIR1, SWIR2). +Each colum represents a different endmember. +Columns represent Landsat bands (R,G,B, NIR, SWIR1, SWIR2). The endmembers can be passed in a single text-file using: @@ -189,7 +215,13 @@ Users can specify additional parameters to configure how the underlying workflow ### Sensor Levels -Data from different satellites can be processed within this workflow. Users may wish to include different satellites in preprocessing and in higher level processing. All input imagery is preprocessed. The `sensors_level2` parameter controls the selection of satellites for the higher level processing steps. The parameter has to follow the FORCE notation for level 2 processing. In particular, a string containing space-separated satellite identifiers has to be supplied (e.g. `"LND04 LND05"` to include Landsat 4 and 5). More details on available satellite identifiers can be found [here](https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html), some common options include: +Data from different satellites can be processed within this workflow. +Users may wish to include different satellites in preprocessing and in higher level processing. +All input imagery is preprocessed. +The `sensors_level2` parameter controls the selection of satellites for the higher level processing steps. +The parameter has to follow the FORCE notation for level 2 processing. +In particular, a string containing space-separated satellite identifiers has to be supplied (e.g. `"LND04 LND05"` to include Landsat 4 and 5). +More details on available satellite identifiers can be found [here](https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html), some common options include: - `"LND04"`: 6-band Landsat 4 TM - `"LND05"`: 6-band Landsat 5 TM @@ -198,7 +230,8 @@ Data from different satellites can be processed within this workflow. Users may - `"SEN2A"`: 10-band Sentinel-2A - `"SEN2B"`: 10-band Sentinel-2B -Note that the specified identifiers have to match the data made available to the workflow. In other words, satellite data for e.g. Landsat 5 can't be processed if it was not supplied using the `input` parameter. +Note that the specified identifiers have to match the data made available to the workflow. +In other words, satellite data for e.g. Landsat 5 can't be processed if it was not supplied using the `input` parameter. The satellite identifiers can be passed as follows: @@ -206,11 +239,18 @@ The satellite identifiers can be passed as follows: --sensors_level2 = '[higher level processing satellite identifier string]' ``` -Note that the parameter is optional and the default value is: `"LND04 LND05 LND07"`. Therefore, by default, the pipeline will use Landsat 4,5 and 7 imagery in higher level processing. +Note that the parameter is optional and the default value is: `"LND04 LND05 LND07"`. +Therefore, by default, the pipeline will use Landsat 4,5 and 7 imagery in higher level processing. ### Resolution -Resolution of satellite imagery defines the real size of a single pixel. As an example, a resolution of 30 meters indicates that a single pixel in the data covers a 30x30 meters square of the earth's surface. Users can customize the resolution that FORCE should assume. This does not necessarily have to match the resolution of the supplied data. FORCE will treat imagery as having the specified resolution. However, passing a resolution not matching the satellite data might lead to unexpected results. Resolution is specified in meters. +Resolution of satellite imagery defines the real size of a single pixel. +For example, a resolution of 30 meters indicates that a single pixel in the data covers a 30x30 meters square of the earth's surface. +Users can customize the resolution that FORCE should assume. +This does not necessarily have to match the resolution of the supplied data. +FORCE will treat imagery as having the specified resolution. +However, passing a resolution not matching the satellite data might lead to unexpected results. +Resolution is specified in meters. A custom resolution can be passed using: @@ -222,7 +262,8 @@ The default value is `30`, as most Landsat satellite natively provide this resol ### Temporal extent -In some scenarios, user may be interested to limit the temporal extent of analysis. To enables this, users can specify both start and end date in a string with this syntax: `'YYYY-MM-DD'`. +In some scenarios, user may be interested to limit the temporal extent of analysis. +To enables this, users can specify both start and end date in a string with this syntax: `'YYYY-MM-DD'`. Start and end date can be passed using: @@ -233,7 +274,10 @@ Start and end date can be passed using: ### Group size -The `group_size` parameters can be ignored in most cases. It defines how many satellite scenes are processed together. The parameters is used to balance the tradeoff between I/O and computational capacities on individual compute nodes. By default, `group_size` is set to `100`. +The `group_size` parameters can be ignored in most cases. +It defines how many satellite scenes are processed together. +The parameters is used to balance the tradeoff between I/O and computational capacities on individual compute nodes. +By default, `group_size` is set to `100`. The group size can be passed using: @@ -243,7 +287,15 @@ The group size can be passed using: ### Higher level processing configuration -During the higher level processing stage, time series analyses of different satellite bands and indexes is performed. The concrete bands and indexes can be defined using the `indexes` parameter. Spectral unmixing is performed in any case. Thus, passing an empty `indexes` parameter will restrict time series analyses to the results of spectral unmixing. All available indexes can be found [here](https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html) above the `INDEX` parameter. The band/index codes need to be passed in a space-separated string. The default value, `indexes = "NDVI BLUE GREEN RED NIR SWIR1 SWIR2"`, enables time series analyses for the NDVI index and the blue, green, red, near-infrared and both shortwave infrared bands. Note that indexes are usually computed based on certain bands. If these bands are not present in the preprocessed data, these indexes can not be computed. +During the higher level processing stage, time series analyses of different satellite bands and indexes is performed. +The concrete bands and indexes can be defined using the `indexes` parameter. +Spectral unmixing is performed in any case. +Thus, passing an empty `indexes` parameter will restrict time series analyses to the results of spectral unmixing. +All available indexes can be found [here](https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html) above the `INDEX` parameter. +The band/index codes need to be passed in a space-separated string. +The default value, `indexes = "NDVI BLUE GREEN RED NIR SWIR1 SWIR2"`, enables time series analyses for the NDVI index and the blue, green, red, near-infrared and both shortwave infrared bands. +Note that indexes are usually computed based on certain bands. +If these bands are not present in the preprocessed data, these indexes can not be computed. The bands and indexes can be passed using: @@ -251,7 +303,10 @@ The bands and indexes can be passed using: --indexes '[index-string]' ``` -In so cases, it may be desirable to analyze the the individual images in a time series. To enable such analysis, the parameter `return_tss` can be used. If set to `true`, the pipeline will return time series stacks for each tile and band combination. The option is disabled by default to reduce the output size. +In so cases, it may be desirable to analyze the the individual images in a time series. +To enable such analysis, the parameter `return_tss` can be used. +If set to `true`, the pipeline will return time series stacks for each tile and band combination. +The option is disabled by default to reduce the output size. The time series stack output can be enabled using: @@ -261,7 +316,12 @@ The time series stack output can be enabled using: ### Visualization -The workflow provides two types of results visualization and aggregation. The fine grained mosaic visualization contains all time series analyses results for all tiles in the original resolution. Pyramid visualizations present a broad overview of the same data but at a lower resolution. Both visualizations can be enabled or disabled using the parameters `mosaic_visualization` and `pyramid_visualization`. By default, both visualization methods are enabled. Note that the mosaic visualization is required to be enabled when using the `test` and `test_full` profiles to allow the pipeline to check the correctness of its results. +The workflow provides two types of results visualization and aggregation. +The fine grained mosaic visualization contains all time series analyses results for all tiles in the original resolution. +Pyramid visualizations present a broad overview of the same data but at a lower resolution. +Both visualizations can be enabled or disabled using the parameters `mosaic_visualization` and `pyramid_visualization`. +By default, both visualization methods are enabled. +Note that the mosaic visualization is required to be enabled when using the `test` and `test_full` profiles to allow the pipeline to check the correctness of its results. The visualizations can be enabled using: @@ -272,7 +332,9 @@ pyramid_visualization = '[boolean]' ### FORCE configuration -FORCE supports parallel computations. Users can specify the number of threads FORCE can spawn for a single preprocessing, or higher level processing process. This is archived through the `force_threads` parameter. +FORCE supports parallel computations. +Users can specify the number of threads FORCE can spawn for a single preprocessing, or higher level processing process. +This is achieved through the `force_threads` parameter. The number of threads can be passed using: @@ -284,7 +346,9 @@ The default value is 2. ### Intermediate data publishing -By default, preprocessing and higher level processing steps do not publish the `.tif` files that they generate to avoid bloating the available memory. However, analysis-ready-data (aka. ARD or level-2 data) and the results of time series analyses (aka. level-3 data) maybe be interesting to certain users. The files for preprocessing and higher level processing can, therefore, be published by setting the `save_ard` and `save_tsa` to `true`, respectively. +By default, preprocessing and higher level processing steps do not publish the `.tif` files that they generate to avoid bloating the available storage. +However, analysis-ready-data (aka. ARD or level-2 data) and the results of time series analyses (aka. level-3 data) maybe be interesting to certain users. +The files for preprocessing and higher level processing can, therefore, be published by setting the `save_ard` and `save_tsa` to `true`, respectively. Publishing of intermediate data can be enabled using: @@ -317,7 +381,8 @@ If you wish to repeatedly use the same parameters for multiple runs, rather than Pipeline settings can be provided in a `yaml` or `json` file via `-params-file `. :::warning -Do not use `-c ` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args). +Do not use `-c ` to specify parameters as this will result in errors. +Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args). ::: The above pipeline run specified with a params file in yaml format: @@ -343,7 +408,9 @@ You can also generate such `YAML`/`JSON` files via [nf-core/launch](https://nf-c ### Updating the pipeline -When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: +When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. +When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. +To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: ```bash nextflow pull nf-core/rangeland @@ -351,9 +418,12 @@ nextflow pull nf-core/rangeland ### Reproducibility -It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. +It is a good idea to specify a pipeline version when running the pipeline on your data. +This ensures that a specific version of the pipeline code and software are used when you run your pipeline. +If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. -First, go to the [nf-core/rangeland releases page](https://github.com/nf-core/rangeland/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. +First, go to the [nf-core/rangeland releases page](https://github.com/nf-core/rangeland/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). +Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports. @@ -371,7 +441,8 @@ These options are part of Nextflow and use a _single_ hyphen (pipeline parameter ### `-profile` -Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. +Use this parameter to choose a configuration profile. +Profiles can give configuration presets for different compute environments. Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Apptainer, Conda) - see below. @@ -379,12 +450,14 @@ Several generic profiles are bundled with the pipeline which instruct the pipeli We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. ::: -The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). +The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. +For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). Note that multiple profiles can be loaded, for example: `-profile test,docker` - the order of arguments is important! They are loaded in sequence, so later profiles can overwrite earlier profiles. -If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended, since it can lead to different results on different machines dependent on the computer enviroment. +If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. +This is _not_ recommended, since it can lead to different results on different machines dependent on the computer enviroment. - `test` - A profile with a complete configuration for automated testing @@ -402,43 +475,58 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof - `apptainer` - A generic configuration profile to be used with [Apptainer](https://apptainer.org/) - `wave` - - A generic configuration profile to enable [Wave](https://seqera.io/wave/) containers. Use together with one of the above (requires Nextflow ` 24.03.0-edge` or later). + - A generic configuration profile to enable [Wave](https://seqera.io/wave/) containers. + Use together with one of the above (requires Nextflow ` 24.03.0-edge` or later). - `conda` - - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer. + - A generic configuration profile to be used with [Conda](https://conda.io/docs/). + Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer. ### `-resume` -Specify this when restarting a pipeline. Nextflow will use cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously. For input to be considered the same, not only the names must be identical but the files' contents as well. For more info about this parameter, see [this blog post](https://www.nextflow.io/blog/2019/demystifying-nextflow-resume.html). +Specify this when restarting a pipeline. +Nextflow will use cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously. +For input to be considered the same, not only the names must be identical but the files' contents as well. +For more info about this parameter, see [this blog post](https://www.nextflow.io/blog/2019/demystifying-nextflow-resume.html). -You can also supply a run name to resume a specific run: `-resume [run-name]`. Use the `nextflow log` command to show previous run names. +You can also supply a run name to resume a specific run: `-resume [run-name]`. +Use the `nextflow log` command to show previous run names. ### `-c` -Specify the path to a specific config file (this is a core Nextflow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information. +Specify the path to a specific config file (this is a core Nextflow command). +See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information. ## Custom configuration ### Resource requests -Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped. +Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. +Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. +For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). +If it still fails after the third attempt then the pipeline execution is stopped. To change the resource requests, please see the [max resources](https://nf-co.re/docs/usage/configuration#max-resources) and [tuning workflow resources](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources) section of the nf-core website. ### Custom Containers -In some cases you may wish to change which container or conda environment a step of the pipeline uses for a particular tool. By default nf-core pipelines use containers and software from the [biocontainers](https://biocontainers.pro/) or [bioconda](https://bioconda.github.io/) projects. However in some cases the pipeline specified version maybe out of date. +In some cases you may wish to change which container or conda environment a step of the pipeline uses for a particular tool. +By default nf-core pipelines use containers and software from the [biocontainers](https://biocontainers.pro/) or [bioconda](https://bioconda.github.io/) projects. +However in some cases the pipeline specified version maybe out of date. To use a different container from the default container or conda environment specified in a pipeline, please see the [updating tool versions](https://nf-co.re/docs/usage/configuration#updating-tool-versions) section of the nf-core website. ### Custom Tool Arguments -A pipeline might not always support every possible argument or option of a particular tool used in pipeline. Fortunately, nf-core pipelines provide some freedom to users to insert additional parameters that the pipeline does not include by default. +A pipeline might not always support every possible argument or option of a particular tool used in pipeline. +Fortunately, nf-core pipelines provide some freedom to users to insert additional parameters that the pipeline does not include by default. To learn how to provide additional arguments to a particular tool of the pipeline, please see the [customising tool arguments](https://nf-co.re/docs/usage/configuration#customising-tool-arguments) section of the nf-core website. ### nf-core/configs -In most cases, you will only need to create a custom config as a one-off but if you and others within your organisation are likely to be running nf-core pipelines regularly and need to use the same settings regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter. You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. +In most cases, you will only need to create a custom config as a one-off but if you and others within your organisation are likely to be running nf-core pipelines regularly and need to use the same settings regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. +Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter. +You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information about creating your own configuration files. @@ -454,9 +542,11 @@ For a thorough list, please refer the [Azure Sizes for virtual machines in Azure ## Running in the background -Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished. +Nextflow handles job submissions and supervises the running jobs. +The Nextflow process must run until the pipeline is finished. -The Nextflow `-bg` flag launches Nextflow in the background, detached from your terminal so that the workflow does not stop if you log out of your session. The logs are saved to a file. +The Nextflow `-bg` flag launches Nextflow in the background, detached from your terminal so that the workflow does not stop if you log out of your session. +The logs are saved to a file. Alternatively, you can use `screen` / `tmux` or similar tool to create a detached session which you can log back into at a later time. Some HPC setups also allow you to run nextflow within a cluster job submitted your job scheduler (from where it submits more jobs). From 17db3baa571e2ccd16b9b713dd96e5ea23b4b667 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Thu, 29 Aug 2024 09:56:36 +0200 Subject: [PATCH 29/61] Removed unnecessarily ignored parameters in test profile --- conf/test.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test.config b/conf/test.config index 8b5506f..d8b1d47 100644 --- a/conf/test.config +++ b/conf/test.config @@ -54,7 +54,7 @@ params { // enable mosaic for result checking mosaic_visualization = true - validationSchemaIgnoreParams = "peak_yoc_ref,peak_change_ref,herbaceous_yoc_ref,herbaceous_change_ref,woody_yoc_ref,woody_change_ref,config_profile_description,config_profile_name" + validationSchemaIgnoreParams = "peak_yoc_ref,peak_change_ref,herbaceous_yoc_ref,herbaceous_change_ref,woody_yoc_ref,woody_change_ref" } process { From 4a7518b9a428f2137976cb98f0160c923b0676ca Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Thu, 29 Aug 2024 10:10:36 +0200 Subject: [PATCH 30/61] Improved parameter references in docs --- docs/output.md | 8 ++++---- docs/usage.md | 36 ++++++++++++++++++------------------ 2 files changed, 22 insertions(+), 22 deletions(-) diff --git a/docs/output.md b/docs/output.md index b13b1d0..93eb93c 100644 --- a/docs/output.md +++ b/docs/output.md @@ -39,7 +39,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d [untar](https://nf-co.re/modules/untar) is a nf-core module used to extract files from tar archives. -Invokation of untar depends on certain parameters (i.e `input_tar`, `dem_tar` and `wvdb_tar`). +Invocation of untar depends on certain parameters (i.e `--input_tar`, `--dem_tar` and `--wvdb_tar`). Thus, the outputs files are only generated when these are set to `true`. ### Preparation @@ -87,7 +87,7 @@ Logs and analysis-ready-data (ARD) are generated using the [force-l2ps](https:// Logs can be consulted for debugging purposes. ARD may be collected as a basis for other remote sensing workflows. The ARD in `level2_ard/` consist two `.tif` files per initial input image, a quality data file and the atmospherically corrected satellite data. -Note that the `.tif` files are only published when the `save_ard` parameter is set to `true` to avoid bloating the storage. +Note that the `.tif` files are only published when the `--save_ard` parameter is set to `true` to avoid bloating the storage. ### Higher-level-Processing @@ -111,9 +111,9 @@ Concretely, a set of endmember (provided using `--endmember`) is exploited to de Next, time series analysis for different vegetation characteristics is performed. The resulting trend files in `trend_files/` can be investigated to view trends for individual tiles. -However, these files are only published if the `save_tsa` parameter is set to `true`. +However, these files are only published if the `--save_tsa` parameter is set to `true`. -If the `return_tss` parameter was set to `true`, the pipeline will also output files with the `TSS` ending. +If the `--return_tss` parameter was set to `true`, the pipeline will also output files with the `TSS` ending. These files contain the time series stack(TSS) for the given tile and index or band. Here, for each date of acquisition, an image is available that contains the values for that date. diff --git a/docs/usage.md b/docs/usage.md index 074b9ae..0c3cf5a 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -116,7 +116,7 @@ In the example above `181036/` and `181035/` would need to be in the top level o A DEM is necessary for topographic correction of Landsat data, and helps to distinguish between cloud, shadows and water surfaces. Common sources for digital elevation models are [Copernicus](https://www.copernicus.eu/en),[Shuttle Radar Topography Mission](https://www2.jpl.nasa.gov/srtm/) (SRTM), or [Advanced Spaceborne Thermal Emission and Reflection Radiometer](https://asterweb.jpl.nasa.gov/) (ASTER). -The pipeline expects a path to the Digital Elevation Model root directory as a parameter. +The pipeline expects a path to the digital elevation model root directory as the `--dem` parameter. Concretely, the expected structure would look like this: ```tree @@ -218,7 +218,7 @@ Users can specify additional parameters to configure how the underlying workflow Data from different satellites can be processed within this workflow. Users may wish to include different satellites in preprocessing and in higher level processing. All input imagery is preprocessed. -The `sensors_level2` parameter controls the selection of satellites for the higher level processing steps. +The `--sensors_level2` parameter controls the selection of satellites for the higher level processing steps. The parameter has to follow the FORCE notation for level 2 processing. In particular, a string containing space-separated satellite identifiers has to be supplied (e.g. `"LND04 LND05"` to include Landsat 4 and 5). More details on available satellite identifiers can be found [here](https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html), some common options include: @@ -231,7 +231,7 @@ More details on available satellite identifiers can be found [here](https://forc - `"SEN2B"`: 10-band Sentinel-2B Note that the specified identifiers have to match the data made available to the workflow. -In other words, satellite data for e.g. Landsat 5 can't be processed if it was not supplied using the `input` parameter. +In other words, satellite data for e.g. Landsat 5 can't be processed if it was not supplied using the `--input` parameter. The satellite identifiers can be passed as follows: @@ -274,10 +274,10 @@ Start and end date can be passed using: ### Group size -The `group_size` parameters can be ignored in most cases. +The `--group_size` parameter can be ignored in most cases. It defines how many satellite scenes are processed together. -The parameters is used to balance the tradeoff between I/O and computational capacities on individual compute nodes. -By default, `group_size` is set to `100`. +The parameter is used to balance the tradeoff between I/O and computational capacities on individual compute nodes. +By default, `--group_size` is set to `100`. The group size can be passed using: @@ -288,12 +288,12 @@ The group size can be passed using: ### Higher level processing configuration During the higher level processing stage, time series analyses of different satellite bands and indexes is performed. -The concrete bands and indexes can be defined using the `indexes` parameter. +The concrete bands and indexes can be defined using the `--indexes` parameter. Spectral unmixing is performed in any case. -Thus, passing an empty `indexes` parameter will restrict time series analyses to the results of spectral unmixing. -All available indexes can be found [here](https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html) above the `INDEX` parameter. +Thus, passing an empty `--indexes` parameter will restrict time series analyses to the results of spectral unmixing. +All available indexes can be found [here](https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html) above the `INDEX` entry. The band/index codes need to be passed in a space-separated string. -The default value, `indexes = "NDVI BLUE GREEN RED NIR SWIR1 SWIR2"`, enables time series analyses for the NDVI index and the blue, green, red, near-infrared and both shortwave infrared bands. +The default value, `--indexes = "NDVI BLUE GREEN RED NIR SWIR1 SWIR2"`, enables time series analyses for the NDVI index and the blue, green, red, near-infrared and both shortwave infrared bands. Note that indexes are usually computed based on certain bands. If these bands are not present in the preprocessed data, these indexes can not be computed. @@ -304,7 +304,7 @@ The bands and indexes can be passed using: ``` In so cases, it may be desirable to analyze the the individual images in a time series. -To enable such analysis, the parameter `return_tss` can be used. +To enable such analysis, the parameter `--return_tss` can be used. If set to `true`, the pipeline will return time series stacks for each tile and band combination. The option is disabled by default to reduce the output size. @@ -319,22 +319,22 @@ The time series stack output can be enabled using: The workflow provides two types of results visualization and aggregation. The fine grained mosaic visualization contains all time series analyses results for all tiles in the original resolution. Pyramid visualizations present a broad overview of the same data but at a lower resolution. -Both visualizations can be enabled or disabled using the parameters `mosaic_visualization` and `pyramid_visualization`. +Both visualizations can be enabled or disabled using the parameters `--mosaic_visualization` and `--pyramid_visualization`. By default, both visualization methods are enabled. Note that the mosaic visualization is required to be enabled when using the `test` and `test_full` profiles to allow the pipeline to check the correctness of its results. The visualizations can be enabled using: ```bash -mosaic_visualization = '[boolean]' -pyramid_visualization = '[boolean]' +--mosaic_visualization = '[boolean]' +--pyramid_visualization = '[boolean]' ``` ### FORCE configuration FORCE supports parallel computations. Users can specify the number of threads FORCE can spawn for a single preprocessing, or higher level processing process. -This is achieved through the `force_threads` parameter. +This is achieved through the `--force_threads` parameter. The number of threads can be passed using: @@ -348,13 +348,13 @@ The default value is 2. By default, preprocessing and higher level processing steps do not publish the `.tif` files that they generate to avoid bloating the available storage. However, analysis-ready-data (aka. ARD or level-2 data) and the results of time series analyses (aka. level-3 data) maybe be interesting to certain users. -The files for preprocessing and higher level processing can, therefore, be published by setting the `save_ard` and `save_tsa` to `true`, respectively. +The files for preprocessing and higher level processing can, therefore, be published by setting the `--save_ard` and `--save_tsa` to `true`, respectively. Publishing of intermediate data can be enabled using: ```bash -save_ard = '[boolean]' -save_tsa = '[boolean]' +--save_ard = '[boolean]' +--save_tsa = '[boolean]' ``` ## Running the pipeline From 494933467891fa5f3ae5fff214cde3b1a6aabcdc Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Fri, 6 Sep 2024 11:48:17 +0200 Subject: [PATCH 31/61] Replaced retry strategies with error_retry label --- conf/modules.config | 13 ++++++------- modules/local/force-higher_level.nf | 1 + modules/local/force-preprocess.nf | 1 + modules/local/higher_level_force_config.nf | 1 + modules/local/merge.nf | 1 + modules/local/preprocess_force_config.nf | 1 + 6 files changed, 11 insertions(+), 7 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index de1c7e4..911dce9 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -35,8 +35,6 @@ process { } withName: "FORCE_PREPROCESS" { - errorStrategy = 'retry' - maxRetries = 5 publishDir = [ [ path: { "${params.outdir}/preprocess/${task.tag}/logs" }, @@ -54,8 +52,6 @@ process { withName: "HIGHER_LEVEL_CONFIG" { - errorStrategy = 'retry' - maxRetries = 5 publishDir = [ path: { "${params.outdir}/higher-level/${task.tag}/param_files" }, mode: params.publish_dir_mode, @@ -98,15 +94,18 @@ process { } withName: "CHECK_RESULTS" { - errorStrategy = { task.exitStatus == 143 ? 'retry' : 'ignore' } + publishDir = [ + enabled: false + ] + } + + withName: "CHECK_RESULTS_FULL" { publishDir = [ enabled: false ] } withName: "PREPROCESS_CONFIG" { - errorStrategy = 'retry' - maxRetries = 5 publishDir = [ path: { "${params.outdir}/preprocess/${task.tag}/param_files" }, mode: params.publish_dir_mode, diff --git a/modules/local/force-higher_level.nf b/modules/local/force-higher_level.nf index 9e91773..6648195 100644 --- a/modules/local/force-higher_level.nf +++ b/modules/local/force-higher_level.nf @@ -1,6 +1,7 @@ process FORCE_HIGHER_LEVEL { tag { tile } label 'process_medium' + label 'error_retry' container "docker.io/davidfrantz/force:3.7.10" diff --git a/modules/local/force-preprocess.nf b/modules/local/force-preprocess.nf index 75f5ae3..d101429 100644 --- a/modules/local/force-preprocess.nf +++ b/modules/local/force-preprocess.nf @@ -1,6 +1,7 @@ process FORCE_PREPROCESS { tag { data.simpleName } label 'process_medium' + label 'error_retry' container "docker.io/davidfrantz/force:3.7.10" diff --git a/modules/local/higher_level_force_config.nf b/modules/local/higher_level_force_config.nf index afcadf4..5b8b9b6 100644 --- a/modules/local/higher_level_force_config.nf +++ b/modules/local/higher_level_force_config.nf @@ -1,6 +1,7 @@ process HIGHER_LEVEL_CONFIG { tag { tile } label 'process_single' + label 'error_retry' container "docker.io/davidfrantz/force:3.7.10" diff --git a/modules/local/merge.nf b/modules/local/merge.nf index 3126637..812a9de 100644 --- a/modules/local/merge.nf +++ b/modules/local/merge.nf @@ -1,6 +1,7 @@ process MERGE { tag { id } label 'process_low' + label 'error_retry' container "docker.io/davidfrantz/force:3.7.10" diff --git a/modules/local/preprocess_force_config.nf b/modules/local/preprocess_force_config.nf index 850675c..8a5a67b 100644 --- a/modules/local/preprocess_force_config.nf +++ b/modules/local/preprocess_force_config.nf @@ -1,6 +1,7 @@ process PREPROCESS_CONFIG { tag { data.simpleName } label 'process_single' + label 'error_retry' container "docker.io/davidfrantz/force:3.7.10" From d7ba83bf4d3eafb4fb3d38278057df46fadf5ef5 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Wed, 18 Sep 2024 10:06:01 +0200 Subject: [PATCH 32/61] Adopted nf-core pseudo-standard directory structure for local modules --- .../local/{check_results.nf => check_results/main.nf} | 0 .../main.nf} | 0 .../main.nf} | 0 .../main.nf} | 0 .../main.nf} | 0 .../local/{force-mosaic.nf => force-mosaic/main.nf} | 0 .../{force-preprocess.nf => force-preprocess/main.nf} | 0 .../local/{force-pyramid.nf => force-pyramid/main.nf} | 0 .../main.nf} | 0 modules/local/{merge.nf => merge/main.nf} | 0 .../main.nf} | 0 subworkflows/local/higher_level.nf | 8 ++++---- subworkflows/local/preprocessing.nf | 10 +++++----- workflows/rangeland.nf | 4 ++-- 14 files changed, 11 insertions(+), 11 deletions(-) rename modules/local/{check_results.nf => check_results/main.nf} (100%) rename modules/local/{check_results_full.nf => check_results_full/main.nf} (100%) rename modules/local/{force-generate_analysis_mask.nf => force-generate_analysis_mask/main.nf} (100%) rename modules/local/{force-generate_tile_allow_list.nf => force-generate_tile_allow_list/main.nf} (100%) rename modules/local/{force-higher_level.nf => force-higher_level/main.nf} (100%) rename modules/local/{force-mosaic.nf => force-mosaic/main.nf} (100%) rename modules/local/{force-preprocess.nf => force-preprocess/main.nf} (100%) rename modules/local/{force-pyramid.nf => force-pyramid/main.nf} (100%) rename modules/local/{higher_level_force_config.nf => higher_level_force_config/main.nf} (100%) rename modules/local/{merge.nf => merge/main.nf} (100%) rename modules/local/{preprocess_force_config.nf => preprocess_force_config/main.nf} (100%) diff --git a/modules/local/check_results.nf b/modules/local/check_results/main.nf similarity index 100% rename from modules/local/check_results.nf rename to modules/local/check_results/main.nf diff --git a/modules/local/check_results_full.nf b/modules/local/check_results_full/main.nf similarity index 100% rename from modules/local/check_results_full.nf rename to modules/local/check_results_full/main.nf diff --git a/modules/local/force-generate_analysis_mask.nf b/modules/local/force-generate_analysis_mask/main.nf similarity index 100% rename from modules/local/force-generate_analysis_mask.nf rename to modules/local/force-generate_analysis_mask/main.nf diff --git a/modules/local/force-generate_tile_allow_list.nf b/modules/local/force-generate_tile_allow_list/main.nf similarity index 100% rename from modules/local/force-generate_tile_allow_list.nf rename to modules/local/force-generate_tile_allow_list/main.nf diff --git a/modules/local/force-higher_level.nf b/modules/local/force-higher_level/main.nf similarity index 100% rename from modules/local/force-higher_level.nf rename to modules/local/force-higher_level/main.nf diff --git a/modules/local/force-mosaic.nf b/modules/local/force-mosaic/main.nf similarity index 100% rename from modules/local/force-mosaic.nf rename to modules/local/force-mosaic/main.nf diff --git a/modules/local/force-preprocess.nf b/modules/local/force-preprocess/main.nf similarity index 100% rename from modules/local/force-preprocess.nf rename to modules/local/force-preprocess/main.nf diff --git a/modules/local/force-pyramid.nf b/modules/local/force-pyramid/main.nf similarity index 100% rename from modules/local/force-pyramid.nf rename to modules/local/force-pyramid/main.nf diff --git a/modules/local/higher_level_force_config.nf b/modules/local/higher_level_force_config/main.nf similarity index 100% rename from modules/local/higher_level_force_config.nf rename to modules/local/higher_level_force_config/main.nf diff --git a/modules/local/merge.nf b/modules/local/merge/main.nf similarity index 100% rename from modules/local/merge.nf rename to modules/local/merge/main.nf diff --git a/modules/local/preprocess_force_config.nf b/modules/local/preprocess_force_config/main.nf similarity index 100% rename from modules/local/preprocess_force_config.nf rename to modules/local/preprocess_force_config/main.nf diff --git a/subworkflows/local/higher_level.nf b/subworkflows/local/higher_level.nf index d0bc922..9cb44b4 100644 --- a/subworkflows/local/higher_level.nf +++ b/subworkflows/local/higher_level.nf @@ -1,7 +1,7 @@ -include { HIGHER_LEVEL_CONFIG } from '../../modules/local/higher_level_force_config.nf' -include { FORCE_HIGHER_LEVEL } from '../../modules/local/force-higher_level.nf' -include { FORCE_MOSAIC } from '../../modules/local/force-mosaic.nf' -include { FORCE_PYRAMID } from '../../modules/local/force-pyramid.nf' +include { HIGHER_LEVEL_CONFIG } from '../../modules/local/higher_level_force_config/main' +include { FORCE_HIGHER_LEVEL } from '../../modules/local/force-higher_level/main' +include { FORCE_MOSAIC } from '../../modules/local/force-mosaic/main' +include { FORCE_PYRAMID } from '../../modules/local/force-pyramid/main' workflow HIGHER_LEVEL { diff --git a/subworkflows/local/preprocessing.nf b/subworkflows/local/preprocessing.nf index 453c1d8..63ef11d 100644 --- a/subworkflows/local/preprocessing.nf +++ b/subworkflows/local/preprocessing.nf @@ -1,8 +1,8 @@ -include { FORCE_GENERATE_TILE_ALLOW_LIST } from '../../modules/local/force-generate_tile_allow_list' -include { FORCE_GENERATE_ANALYSIS_MASK } from '../../modules/local/force-generate_analysis_mask' -include { PREPROCESS_CONFIG } from '../../modules/local/preprocess_force_config' -include { FORCE_PREPROCESS } from '../../modules/local/force-preprocess' -include { MERGE as MERGE_BOA; MERGE as MERGE_QAI } from '../../modules/local/merge' +include { FORCE_GENERATE_TILE_ALLOW_LIST } from '../../modules/local/force-generate_tile_allow_list/main' +include { FORCE_GENERATE_ANALYSIS_MASK } from '../../modules/local/force-generate_analysis_mask/main' +include { PREPROCESS_CONFIG } from '../../modules/local/preprocess_force_config/main' +include { FORCE_PREPROCESS } from '../../modules/local/force-preprocess/main' +include { MERGE as MERGE_BOA; MERGE as MERGE_QAI } from '../../modules/local/merge/main' // Closure to extract the parent directory of a file def extractDirectory = { it.parent.toString().substring(it.parent.toString().lastIndexOf('/') + 1 ) } diff --git a/workflows/rangeland.nf b/workflows/rangeland.nf index dd01dfd..b3bb782 100644 --- a/workflows/rangeland.nf +++ b/workflows/rangeland.nf @@ -26,8 +26,8 @@ include { HIGHER_LEVEL } from '../subworkflows/local/higher_level' // MODULES // -include { CHECK_RESULTS } from '../modules/local/check_results' -include { CHECK_RESULTS_FULL } from '../modules/local/check_results_full' +include { CHECK_RESULTS } from '../modules/local/check_results/main' +include { CHECK_RESULTS_FULL } from '../modules/local/check_results_full/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT NF-CORE MODULES/SUBWORKFLOWS From 64c909acf1e0a2a560a010b92b6f1fe950305be0 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Wed, 18 Sep 2024 10:23:55 +0200 Subject: [PATCH 33/61] Updated nf-core untar module --- modules.json | 2 +- modules/nf-core/untar/environment.yml | 7 + modules/nf-core/untar/main.nf | 31 +++- modules/nf-core/untar/tests/main.nf.test | 85 ++++++++++ modules/nf-core/untar/tests/main.nf.test.snap | 158 ++++++++++++++++++ modules/nf-core/untar/tests/tags.yml | 2 + 6 files changed, 279 insertions(+), 6 deletions(-) create mode 100644 modules/nf-core/untar/environment.yml create mode 100644 modules/nf-core/untar/tests/main.nf.test create mode 100644 modules/nf-core/untar/tests/main.nf.test.snap create mode 100644 modules/nf-core/untar/tests/tags.yml diff --git a/modules.json b/modules.json index 9e83e0b..fa2f544 100644 --- a/modules.json +++ b/modules.json @@ -12,7 +12,7 @@ }, "untar": { "branch": "master", - "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", + "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", "installed_by": ["modules"] } } diff --git a/modules/nf-core/untar/environment.yml b/modules/nf-core/untar/environment.yml new file mode 100644 index 0000000..c779485 --- /dev/null +++ b/modules/nf-core/untar/environment.yml @@ -0,0 +1,7 @@ +channels: + - conda-forge + - bioconda +dependencies: + - conda-forge::grep=3.11 + - conda-forge::sed=4.8 + - conda-forge::tar=1.34 diff --git a/modules/nf-core/untar/main.nf b/modules/nf-core/untar/main.nf index 61461c3..9bd8f55 100644 --- a/modules/nf-core/untar/main.nf +++ b/modules/nf-core/untar/main.nf @@ -2,10 +2,10 @@ process UNTAR { tag "$archive" label 'process_single' - conda "conda-forge::sed=4.7 conda-forge::grep=3.11 conda-forge::tar=1.34" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : - 'nf-core/ubuntu:20.04' }" + 'https://depot.galaxyproject.org/singularity/ubuntu:22.04' : + 'nf-core/ubuntu:22.04' }" input: tuple val(meta), path(archive) @@ -52,8 +52,29 @@ process UNTAR { stub: prefix = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.toString().replaceFirst(/\.[^\.]+(.gz)?$/, "")) """ - mkdir $prefix - touch ${prefix}/file.txt + mkdir ${prefix} + ## Dry-run untaring the archive to get the files and place all in prefix + if [[ \$(tar -taf ${archive} | grep -o -P "^.*?\\/" | uniq | wc -l) -eq 1 ]]; then + for i in `tar -tf ${archive}`; + do + if [[ \$(echo "\${i}" | grep -E "/\$") == "" ]]; + then + touch \${i} + else + mkdir -p \${i} + fi + done + else + for i in `tar -tf ${archive}`; + do + if [[ \$(echo "\${i}" | grep -E "/\$") == "" ]]; + then + touch ${prefix}/\${i} + else + mkdir -p ${prefix}/\${i} + fi + done + fi cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/untar/tests/main.nf.test b/modules/nf-core/untar/tests/main.nf.test new file mode 100644 index 0000000..c957517 --- /dev/null +++ b/modules/nf-core/untar/tests/main.nf.test @@ -0,0 +1,85 @@ +nextflow_process { + + name "Test Process UNTAR" + script "../main.nf" + process "UNTAR" + tag "modules" + tag "modules_nfcore" + tag "untar" + + test("test_untar") { + + when { + process { + """ + input[0] = [ [], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/db/kraken2.tar.gz', checkIfExists: true) ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + ) + } + } + + test("test_untar_onlyfiles") { + + when { + process { + """ + input[0] = [ [], file(params.modules_testdata_base_path + 'generic/tar/hello.tar.gz', checkIfExists: true) ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + ) + } + } + + test("test_untar - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ [], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/db/kraken2.tar.gz', checkIfExists: true) ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + ) + } + } + + test("test_untar_onlyfiles - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ [], file(params.modules_testdata_base_path + 'generic/tar/hello.tar.gz', checkIfExists: true) ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + ) + } + } +} diff --git a/modules/nf-core/untar/tests/main.nf.test.snap b/modules/nf-core/untar/tests/main.nf.test.snap new file mode 100644 index 0000000..ceb91b7 --- /dev/null +++ b/modules/nf-core/untar/tests/main.nf.test.snap @@ -0,0 +1,158 @@ +{ + "test_untar_onlyfiles": { + "content": [ + { + "0": [ + [ + [ + + ], + [ + "hello.txt:md5,e59ff97941044f85df5297e1c302d260" + ] + ] + ], + "1": [ + "versions.yml:md5,6063247258c56fd271d076bb04dd7536" + ], + "untar": [ + [ + [ + + ], + [ + "hello.txt:md5,e59ff97941044f85df5297e1c302d260" + ] + ] + ], + "versions": [ + "versions.yml:md5,6063247258c56fd271d076bb04dd7536" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-10T12:04:28.231047" + }, + "test_untar_onlyfiles - stub": { + "content": [ + { + "0": [ + [ + [ + + ], + [ + "hello.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ], + "1": [ + "versions.yml:md5,6063247258c56fd271d076bb04dd7536" + ], + "untar": [ + [ + [ + + ], + [ + "hello.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ], + "versions": [ + "versions.yml:md5,6063247258c56fd271d076bb04dd7536" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-10T12:04:45.773103" + }, + "test_untar - stub": { + "content": [ + { + "0": [ + [ + [ + + ], + [ + "hash.k2d:md5,d41d8cd98f00b204e9800998ecf8427e", + "opts.k2d:md5,d41d8cd98f00b204e9800998ecf8427e", + "taxo.k2d:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ], + "1": [ + "versions.yml:md5,6063247258c56fd271d076bb04dd7536" + ], + "untar": [ + [ + [ + + ], + [ + "hash.k2d:md5,d41d8cd98f00b204e9800998ecf8427e", + "opts.k2d:md5,d41d8cd98f00b204e9800998ecf8427e", + "taxo.k2d:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ], + "versions": [ + "versions.yml:md5,6063247258c56fd271d076bb04dd7536" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-10T12:04:36.777441" + }, + "test_untar": { + "content": [ + { + "0": [ + [ + [ + + ], + [ + "hash.k2d:md5,8b8598468f54a7087c203ad0190555d9", + "opts.k2d:md5,a033d00cf6759407010b21700938f543", + "taxo.k2d:md5,094d5891cdccf2f1468088855c214b2c" + ] + ] + ], + "1": [ + "versions.yml:md5,6063247258c56fd271d076bb04dd7536" + ], + "untar": [ + [ + [ + + ], + [ + "hash.k2d:md5,8b8598468f54a7087c203ad0190555d9", + "opts.k2d:md5,a033d00cf6759407010b21700938f543", + "taxo.k2d:md5,094d5891cdccf2f1468088855c214b2c" + ] + ] + ], + "versions": [ + "versions.yml:md5,6063247258c56fd271d076bb04dd7536" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-10T12:04:19.377674" + } +} \ No newline at end of file diff --git a/modules/nf-core/untar/tests/tags.yml b/modules/nf-core/untar/tests/tags.yml new file mode 100644 index 0000000..feb6f15 --- /dev/null +++ b/modules/nf-core/untar/tests/tags.yml @@ -0,0 +1,2 @@ +untar: + - modules/nf-core/untar/** From 5e006c0330ce8f530903d712503bc5f0753e32ad Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Wed, 18 Sep 2024 12:00:02 +0200 Subject: [PATCH 34/61] Enriched schema with patterns for parameters --- docs/usage.md | 1 + nextflow_schema.json | 37 ++++++++++++++++++++++++++----------- 2 files changed, 27 insertions(+), 11 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 0c3cf5a..06ce2fd 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -178,6 +178,7 @@ The datacube definition is passed as a single file using: ### Area of interest (AOI) The area of interest is a geospatial vector dataset that holds the boundary of the targeted area. +The file must be a shapefile or geopackage vector file. AOI is passed as a single file using: diff --git a/nextflow_schema.json b/nextflow_schema.json index 4fb0b6d..26dad4a 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -17,7 +17,8 @@ "fa_icon": "fas fa-satellite", "help_text": "Set this to the root directory of all satellite imagery. Directory structure should match format of data downloaded with '$ force-level1-csd\n' For concrete directory structure see [usage documentation](https://nf-co.re/rangeland/docs/usage)", "description": "Root directory of all satellite imagery.", - "mimetype": "application/x-tar" + "mimetype": "application/x-tar", + "format": "path" }, "input_tar": { "type": "boolean", @@ -29,7 +30,8 @@ "type": "string", "help_text": "Path to directory containing a subdirectory with tile-wise digital elevation files (`.tif`) and a virtual dataset file (`.vrt` file) comprising all tile-wise files from the subdirectory.", "fa_icon": "fas fa-mountain", - "description": "Digital elevation model." + "description": "Digital elevation model.", + "format": "path" }, "dem_tar": { "type": "boolean", @@ -41,7 +43,8 @@ "type": "string", "fa_icon": "fas fa-burn", "description": "Water vapor dataset.", - "help_text": "Directory containing a number text files describing global water vapor data at different timestamps, and a coordinate order (`.coo`-)file containing the reference system of the water vapor data." + "help_text": "Directory containing a number text files describing global water vapor data at different timestamps, and a coordinate order (`.coo`-)file containing the reference system of the water vapor data.", + "format": "path" }, "wvdb_tar": { "type": "boolean", @@ -53,19 +56,26 @@ "type": "string", "description": "Datacube definition.", "fa_icon": "fas fa-cube", - "help_text": "A single `.prj` file describing the projection and reference grid of the generated datacube." + "help_text": "A single `.prj` file describing the projection and reference grid of the generated datacube.", + "pattern": "^\\S+\\.prj$", + "format": "file-path" }, "aoi": { "type": "string", "fa_icon": "fas fa-chart-area", "description": "Area of interest.", - "help_text": "A single vector file specifying the spatial extend of the targeted area." + "help_text": "A single vector file specifying the spatial extend of the targeted area.", + "pattern": "^\\S+\\.(gpkg|shp)$", + "format": "file-path" }, "endmember": { "type": "string", "fa_icon": "fas fa-chart-line", "description": "Endmember definition.", - "help_text": "A single text file specifying where rows correspond to satellite bands and columns correspond to endmembers. Values correspond to reflectance values and are separated by spaces." + "help_text": "A single text file specifying where rows correspond to satellite bands and columns correspond to endmembers. Values correspond to reflectance values and are separated by spaces.", + "pattern": "^\\S+\\.txt$", + "format": "file-path", + "mimetype": "text/plain" }, "outdir": { "type": "string", @@ -99,21 +109,24 @@ "default": "LND04 LND05 LND07", "fa_icon": "fas fa-satellite", "description": "Satellites for which data should be incorporated into higher level processing.", - "help_text": "String containing space-separated indicators of satellites in FORCE level 2 format. Common options:\n\"LND04\": 6-band Landsat 4 TM, \n\"LND05\": 6-band Landsat 5 TM, \n\"LND07\": 6-band Landsat 7 ETM+,\n\"LND08/09\": 6-band Landsat 8-9 OLI, \n\"SEN2A\": 10-band Sentinel-2A, \n\"SEN2B\": 10-band Sentinel-2B,\nall options [here](https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html) \n" + "help_text": "String containing space-separated indicators of satellites in FORCE level 2 format. Common options:\n\"LND04\": 6-band Landsat 4 TM, \n\"LND05\": 6-band Landsat 5 TM, \n\"LND07\": 6-band Landsat 7 ETM+,\n\"LND08/09\": 6-band Landsat 8-9 OLI, \n\"SEN2A\": 10-band Sentinel-2A, \n\"SEN2B\": 10-band Sentinel-2B,\nall options [here](https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html) \n", + "pattern": "^((LND04|LND05|LND07|LND08|LND09|SEN2A|SEN2B|sen2a|sen2b|S1AIA|S1BIA|S1AID|S1BID|MOD01|MOD02|LNDLG|SEN2L|SEN2H|R-G-B|VVVHP|MODIS)(\\s|$))+$" }, "start_date": { "type": "string", "default": "1984-01-01", "fa_icon": "far fa-calendar-alt", "description": "First day of interest.", - "help_text": "String with format: \"YYYY-MM-DD\"." + "help_text": "String with format: \"YYYY-MM-DD\".", + "pattern": "^\\d{4}-\\d{2}-\\d{2}$" }, "end_date": { "type": "string", "default": "2006-12-31", "fa_icon": "far fa-calendar-alt", "description": "Last day of interest.", - "help_text": "String with format: \"YYYY-MM-DD\"." + "help_text": "String with format: \"YYYY-MM-DD\".", + "pattern": "^\\d{4}-\\d{2}-\\d{2}$" }, "resolution": { "type": "integer", @@ -134,7 +147,8 @@ "default": "NDVI BLUE GREEN RED NIR SWIR1 SWIR2", "help_text": "Space-separated list of indexes and bands that should be considered in time series analyses. They are indicated by using their established abbreviations. The full list of available indexes is available at https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html under the 'INDEX' parameter. Spectral unmixing is a special index and always activated.", "description": "Select which bands and indexes should be considered in time series analyses.", - "fa_icon": "fas fa-satellite" + "fa_icon": "fas fa-satellite", + "pattern": "((BLUE|GREEN|RED|NIR|SWIR1|SWIR2|RE1|RE2|RE3|BNIR|NDVI|EVI|NBR|NDTI|ARVI|SAVI|SARVI|TC-BRIGHT|TC-GREEN|TC-WET|TC-DI|NDBI|NDWI|MNDWI|NDMI|NDSI|SMA|kNDVI|NDRE1|NDRE2|CIre|NDVIre1|NDVIre2|NDVIre3|NDVIre1n|NDVIre2n|NDVIre3n|MSRre|MSRren,CCI)(\\s|$))+" }, "return_tss": { "type": "boolean", @@ -207,7 +221,8 @@ "type": "integer", "default": 2, "description": "Number of threads spawned by FORCE for each higher-level or preprocessing task.", - "fa_icon": "fas fa-microchip" + "fa_icon": "fas fa-microchip", + "minimum": 1 } }, "fa_icon": "fas fa-terminal", From 980347ffa88005da5f62751ed42d666ca098ad39 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Wed, 18 Sep 2024 16:03:33 +0200 Subject: [PATCH 35/61] Improved output documentation for tss files --- docs/output.md | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/docs/output.md b/docs/output.md index 93eb93c..00ba296 100644 --- a/docs/output.md +++ b/docs/output.md @@ -97,6 +97,7 @@ Note that the `.tif` files are only published when the `--save_ard` parameter is - `higher-level//` - `param_files/`: Parameter files used in [force-higher-level](https://force-eo.readthedocs.io/en/latest/components/higher-level/index.html). - `trend_files/`: Symlinks to trend files that are the result of higher-level processing. + This may optionally contain the time series stack. @@ -113,9 +114,10 @@ Next, time series analysis for different vegetation characteristics is performed The resulting trend files in `trend_files/` can be investigated to view trends for individual tiles. However, these files are only published if the `--save_tsa` parameter is set to `true`. -If the `--return_tss` parameter was set to `true`, the pipeline will also output files with the `TSS` ending. +If the `--return_tss` parameter was set to `true`, the pipeline will also output `.tif` files with the `TSS` in their name. These files contain the time series stack(TSS) for the given tile and index or band. Here, for each date of acquisition, an image is available that contains the values for that date. +TSS files will not be returned if `--save_tsa` is set to `false`. ### Visualization From e036cb30ad292f993c1bae1161481c6ef7ee1c01 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Wed, 18 Sep 2024 16:25:20 +0200 Subject: [PATCH 36/61] Added date format to date parameters --- nextflow_schema.json | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 26dad4a..c15af33 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -118,7 +118,8 @@ "fa_icon": "far fa-calendar-alt", "description": "First day of interest.", "help_text": "String with format: \"YYYY-MM-DD\".", - "pattern": "^\\d{4}-\\d{2}-\\d{2}$" + "pattern": "^\\d{4}-\\d{2}-\\d{2}$", + "format": "date" }, "end_date": { "type": "string", @@ -126,7 +127,8 @@ "fa_icon": "far fa-calendar-alt", "description": "Last day of interest.", "help_text": "String with format: \"YYYY-MM-DD\".", - "pattern": "^\\d{4}-\\d{2}-\\d{2}$" + "pattern": "^\\d{4}-\\d{2}-\\d{2}$", + "format": "date" }, "resolution": { "type": "integer", From 7564dd6affa928b68ee74bde053483fe4fd8f1b1 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Wed, 18 Sep 2024 16:32:28 +0200 Subject: [PATCH 37/61] Removed deprecated docker parameter --- nextflow.config | 1 - 1 file changed, 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 2aacdca..2136a94 100644 --- a/nextflow.config +++ b/nextflow.config @@ -130,7 +130,6 @@ profiles { } docker { docker.enabled = true - docker.userEmulation = true conda.enabled = false singularity.enabled = false podman.enabled = false From cd5fb92e047bd30051606d57845c217e9376c9da Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Thu, 19 Sep 2024 11:40:27 +0200 Subject: [PATCH 38/61] Added tags to all modules --- modules/local/check_results/main.nf | 1 + modules/local/check_results_full/main.nf | 1 + modules/local/force-generate_analysis_mask/main.nf | 1 + modules/local/force-generate_tile_allow_list/main.nf | 1 + 4 files changed, 4 insertions(+) diff --git a/modules/local/check_results/main.nf b/modules/local/check_results/main.nf index 4b1e14c..def8337 100644 --- a/modules/local/check_results/main.nf +++ b/modules/local/check_results/main.nf @@ -1,4 +1,5 @@ process CHECK_RESULTS { + tag 'check' label 'process_low' container 'docker.io/rocker/geospatial:4.3.1' diff --git a/modules/local/check_results_full/main.nf b/modules/local/check_results_full/main.nf index a4c4898..f1f094b 100644 --- a/modules/local/check_results_full/main.nf +++ b/modules/local/check_results_full/main.nf @@ -1,4 +1,5 @@ process CHECK_RESULTS_FULL { + tag 'check' label 'process_low' container 'docker.io/rocker/geospatial:4.3.1' diff --git a/modules/local/force-generate_analysis_mask/main.nf b/modules/local/force-generate_analysis_mask/main.nf index b9297fe..0460853 100644 --- a/modules/local/force-generate_analysis_mask/main.nf +++ b/modules/local/force-generate_analysis_mask/main.nf @@ -1,4 +1,5 @@ process FORCE_GENERATE_ANALYSIS_MASK{ + tag { aoi.simpleName } label 'process_single' container "docker.io/davidfrantz/force:3.7.10" diff --git a/modules/local/force-generate_tile_allow_list/main.nf b/modules/local/force-generate_tile_allow_list/main.nf index fa1210c..6125e47 100644 --- a/modules/local/force-generate_tile_allow_list/main.nf +++ b/modules/local/force-generate_tile_allow_list/main.nf @@ -1,4 +1,5 @@ process FORCE_GENERATE_TILE_ALLOW_LIST{ + tag { aoi.simpleName } label 'process_single' container "docker.io/davidfrantz/force:3.7.10" From f12f0c9b5ddf5c6e30841e265f850a5b6bec0ccd Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Thu, 26 Sep 2024 09:26:54 +0200 Subject: [PATCH 39/61] Removed restrictions on FORCE thread numbers and corresponding parameter --- docs/usage.md | 14 ------------- modules/local/force-higher_level/main.nf | 2 +- .../local/higher_level_force_config/main.nf | 8 +------- modules/local/preprocess_force_config/main.nf | 1 - nextflow.config | 3 --- nextflow_schema.json | 20 ------------------- 6 files changed, 2 insertions(+), 46 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 06ce2fd..0244faa 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -331,20 +331,6 @@ The visualizations can be enabled using: --pyramid_visualization = '[boolean]' ``` -### FORCE configuration - -FORCE supports parallel computations. -Users can specify the number of threads FORCE can spawn for a single preprocessing, or higher level processing process. -This is achieved through the `--force_threads` parameter. - -The number of threads can be passed using: - -```bash ---force_threads '[integer]' -``` - -The default value is 2. - ### Intermediate data publishing By default, preprocessing and higher level processing steps do not publish the `.tif` files that they generate to avoid bloating the available storage. diff --git a/modules/local/force-higher_level/main.nf b/modules/local/force-higher_level/main.nf index 6648195..f174453 100644 --- a/modules/local/force-higher_level/main.nf +++ b/modules/local/force-higher_level/main.nf @@ -21,7 +21,7 @@ process FORCE_HIGHER_LEVEL { mkdir trend - # set provenance + # set provenance directory mkdir prov sed -i "/^DIR_PROVENANCE /c\\DIR_PROVENANCE = prov/" \$PARAM diff --git a/modules/local/higher_level_force_config/main.nf b/modules/local/higher_level_force_config/main.nf index 5b8b9b6..8e7a42b 100644 --- a/modules/local/higher_level_force_config/main.nf +++ b/modules/local/higher_level_force_config/main.nf @@ -25,19 +25,13 @@ process HIGHER_LEVEL_CONFIG { # set parameters - #Replace pathes + # Replace paths sed -i "/^DIR_LOWER /c\\DIR_LOWER = ard/" \$PARAM sed -i "/^DIR_HIGHER /c\\DIR_HIGHER = trend/" \$PARAM sed -i "/^DIR_MASK /c\\DIR_MASK = mask/" \$PARAM sed -i "/^BASE_MASK /c\\BASE_MASK = aoi.tif" \$PARAM sed -i "/^FILE_ENDMEM /c\\FILE_ENDMEM = $endmember" \$PARAM - # threading - sed -i "/^NTHREAD_READ /c\\NTHREAD_READ = 1" \$PARAM # might need some modification - sed -i "/^NTHREAD_COMPUTE /c\\NTHREAD_COMPUTE = $params.force_threads" \$PARAM # might need some modification - sed -i "/^NTHREAD_WRITE /c\\NTHREAD_WRITE = 1" \$PARAM # might need some modification - - # replace Tile to process TILE="$tile" X=\${TILE:1:4} diff --git a/modules/local/preprocess_force_config/main.nf b/modules/local/preprocess_force_config/main.nf index 8a5a67b..ff30e9c 100644 --- a/modules/local/preprocess_force_config/main.nf +++ b/modules/local/preprocess_force_config/main.nf @@ -47,7 +47,6 @@ process PREPROCESS_CONFIG { sed -i "/^ORIGIN_LON /c\\ORIGIN_LON = \$ORIGINX" \$PARAM sed -i "/^ORIGIN_LAT /c\\ORIGIN_LAT = \$ORIGINY" \$PARAM sed -i "/^PROJECTION /c\\PROJECTION = \$CRS" \$PARAM - sed -i "/^NTHREAD /c\\NTHREAD = $params.force_threads" \$PARAM cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/nextflow.config b/nextflow.config index 2136a94..940ca41 100644 --- a/nextflow.config +++ b/nextflow.config @@ -32,9 +32,6 @@ params { save_ard = false save_tsa = false - // FORCE - force_threads = 2 - // Higher Level processing configuration indexes = "NDVI BLUE GREEN RED NIR SWIR1 SWIR2" return_tss = false diff --git a/nextflow_schema.json b/nextflow_schema.json index c15af33..c1f7d19 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -213,23 +213,6 @@ "help_text": "You can savely use the defaults.", "fa_icon": "fas fa-project-diagram" }, - "force_parameters": { - "title": "FORCE parameters", - "type": "object", - "description": "Parameters specific to the FORCE tool.", - "default": "", - "properties": { - "force_threads": { - "type": "integer", - "default": 2, - "description": "Number of threads spawned by FORCE for each higher-level or preprocessing task.", - "fa_icon": "fas fa-microchip", - "minimum": 1 - } - }, - "fa_icon": "fas fa-terminal", - "help_text": "" - }, "institutional_config_options": { "title": "Institutional config options", "type": "object", @@ -448,9 +431,6 @@ { "$ref": "#/definitions/workflow_configuration" }, - { - "$ref": "#/definitions/force_parameters" - }, { "$ref": "#/definitions/institutional_config_options" }, From 7f7805b20ef109326d306cb8e1b54864d58f4dca Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Thu, 26 Sep 2024 10:55:47 +0200 Subject: [PATCH 40/61] Replaced usage of params in modules and subworkflows with channels --- .../force-generate_analysis_mask/main.nf | 3 ++- .../local/higher_level_force_config/main.nf | 16 +++++++++---- subworkflows/local/higher_level.nf | 24 ++++++++++++++++--- subworkflows/local/preprocessing.nf | 8 ++++--- workflows/rangeland.nf | 24 +++++++++++++++++-- 5 files changed, 61 insertions(+), 14 deletions(-) diff --git a/modules/local/force-generate_analysis_mask/main.nf b/modules/local/force-generate_analysis_mask/main.nf index 0460853..fdaa5a9 100644 --- a/modules/local/force-generate_analysis_mask/main.nf +++ b/modules/local/force-generate_analysis_mask/main.nf @@ -7,6 +7,7 @@ process FORCE_GENERATE_ANALYSIS_MASK{ input: path aoi path 'mask/datacube-definition.prj' + val resolution output: //Mask for whole region @@ -18,7 +19,7 @@ process FORCE_GENERATE_ANALYSIS_MASK{ script: """ - force-cube -o mask/ -s $params.resolution $aoi + force-cube -o mask/ -s $resolution $aoi cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/local/higher_level_force_config/main.nf b/modules/local/higher_level_force_config/main.nf index 8e7a42b..dbcc9fe 100644 --- a/modules/local/higher_level_force_config/main.nf +++ b/modules/local/higher_level_force_config/main.nf @@ -9,6 +9,12 @@ process HIGHER_LEVEL_CONFIG { tuple val(tile), path("ard/${tile}/*"), path("ard/${tile}/*"), path("mask/${tile}/aoi.tif") path 'ard/datacube-definition.prj' path endmember + val resolution + val sensors_level2 + val start_date + val end_date + val indexes + val return_tss output: tuple val (tile), path("trend_${tile}.prm"), path("ard/", includeInputs: true), path("mask/", includeInputs: true), path('ard/datacube-definition.prj', includeInputs: true), path(endmember, includeInputs: true), emit: higher_level_configs_and_data @@ -40,20 +46,20 @@ process HIGHER_LEVEL_CONFIG { sed -i "/^Y_TILE_RANGE /c\\Y_TILE_RANGE = \$Y \$Y" \$PARAM # resolution - sed -i "/^RESOLUTION /c\\RESOLUTION = $params.resolution" \$PARAM + sed -i "/^RESOLUTION /c\\RESOLUTION = $resolution" \$PARAM # sensors - sed -i "/^SENSORS /c\\SENSORS = $params.sensors_level2" \$PARAM + sed -i "/^SENSORS /c\\SENSORS = $sensors_level2" \$PARAM # date range - sed -i "/^DATE_RANGE /c\\DATE_RANGE = $params.start_date $params.end_date" \$PARAM + sed -i "/^DATE_RANGE /c\\DATE_RANGE = $start_date $end_date" \$PARAM # spectral index - sed -i "/^INDEX /c\\INDEX = SMA $params.indexes" \$PARAM - ${ params.return_tss ? 'sed -i "/^OUTPUT_TSS /c\\OUTPUT_TSS = TRUE" \$PARAM' : '' } + sed -i "/^INDEX /c\\INDEX = SMA $indexes" \$PARAM + ${ return_tss ? 'sed -i "/^OUTPUT_TSS /c\\OUTPUT_TSS = TRUE" \$PARAM' : '' } # interpolation sed -i "/^INT_DAY /c\\INT_DAY = 8" \$PARAM diff --git a/subworkflows/local/higher_level.nf b/subworkflows/local/higher_level.nf index 9cb44b4..ecaa94e 100644 --- a/subworkflows/local/higher_level.nf +++ b/subworkflows/local/higher_level.nf @@ -9,13 +9,31 @@ workflow HIGHER_LEVEL { tiles_and_masks cube_file endmember_file + mosaic_visualization + pyramid_visualization + resolution + sensors_level2 + start_date + end_date + indexes + return_tss main: ch_versions = Channel.empty() // create configuration file for higher level processing - HIGHER_LEVEL_CONFIG( tiles_and_masks, cube_file, endmember_file ) + HIGHER_LEVEL_CONFIG ( + tiles_and_masks, + cube_file, + endmember_file, + resolution, + sensors_level2, + start_date, + end_date, + indexes, + return_tss + ) ch_versions = ch_versions.mix(HIGHER_LEVEL_CONFIG.out.versions.first()) // main processing @@ -28,13 +46,13 @@ workflow HIGHER_LEVEL { // visualizations mosaic_files = Channel.empty() - if (params.mosaic_visualization) { + if (mosaic_visualization) { FORCE_MOSAIC( trend_files_mosaic, cube_file ) mosaic_files = FORCE_MOSAIC.out.trend_files ch_versions = ch_versions.mix(FORCE_MOSAIC.out.versions.first()) } - if (params.pyramid_visualization) { + if (pyramid_visualization) { FORCE_PYRAMID( trend_files.filter { it[1].name.endsWith('.tif') }.map { [ it[1].simpleName.substring(0,11), it[1] ] } .groupTuple() ) ch_versions = ch_versions.mix(FORCE_PYRAMID.out.versions.first()) } diff --git a/subworkflows/local/preprocessing.nf b/subworkflows/local/preprocessing.nf index 63ef11d..0ea00d2 100644 --- a/subworkflows/local/preprocessing.nf +++ b/subworkflows/local/preprocessing.nf @@ -15,6 +15,8 @@ workflow PREPROCESSING { wvdb cube_file aoi_file + group_size + resolution main: @@ -23,7 +25,7 @@ workflow PREPROCESSING { FORCE_GENERATE_TILE_ALLOW_LIST( aoi_file, cube_file ) ch_versions = ch_versions.mix(FORCE_GENERATE_TILE_ALLOW_LIST.out.versions) - FORCE_GENERATE_ANALYSIS_MASK( aoi_file, cube_file ) + FORCE_GENERATE_ANALYSIS_MASK( aoi_file, cube_file, resolution ) ch_versions = ch_versions.mix(FORCE_GENERATE_ANALYSIS_MASK.out.versions) //Group masks by tile @@ -47,14 +49,14 @@ workflow PREPROCESSING { //Sort to ensure the same groups if you use resume .toSortedList{ a,b -> a[1][0].simpleName <=> b[1][0].simpleName } .flatMap{it} - .groupTuple( remainder : true, size : params.group_size ).map{ [ it[0], it[1] .flatten() ] } + .groupTuple( remainder : true, size : group_size ).map{ [ it[0], it[1] .flatten() ] } qai_tiles_to_merge = qai_tiles.filter{ x -> x[1].size() > 1 } .map{ [ it[0].substring( 0, 11 ), it[1] ] } //Sort to ensure the same groups if you use resume .toSortedList{ a,b -> a[1][0].simpleName <=> b[1][0].simpleName } .flatMap{it} - .groupTuple( remainder : true, size : params.group_size ).map{ [ it[0], it[1] .flatten() ] } + .groupTuple( remainder : true, size : group_size ).map{ [ it[0], it[1] .flatten() ] } //Find tiles with only one file boa_tiles_done = boa_tiles.filter{ x -> x[1].size() == 1 }.map{ x -> [ x[0] .substring( 0, 11 ), x[1][0] ] } diff --git a/workflows/rangeland.nf b/workflows/rangeland.nf index b3bb782..a83866e 100644 --- a/workflows/rangeland.nf +++ b/workflows/rangeland.nf @@ -118,13 +118,33 @@ workflow RANGELAND { // // SUBWORKFLOW: Preprocess satellite imagery // - PREPROCESSING(data, dem, wvdb, cube_file, aoi_file) + PREPROCESSING ( + data, + dem, + wvdb, + cube_file, + aoi_file, + params.group_size, + params.resolution + ) ch_versions = ch_versions.mix(PREPROCESSING.out.versions) // // SUBWORKFLOW: Generate trend files and visualization // - HIGHER_LEVEL(PREPROCESSING.out.tiles_and_masks, cube_file, endmember_file) + HIGHER_LEVEL( + PREPROCESSING.out.tiles_and_masks, + cube_file, + endmember_file, + params.mosaic_visualization, + params.pyramid_visualization, + params.resolution, + params.sensors_level2, + params.start_date, + params.end_date, + params.indexes, + params.return_tss + ) ch_versions = ch_versions.mix(HIGHER_LEVEL.out.versions) grouped_trend_data = HIGHER_LEVEL.out.mosaic_files.map{ it[1] }.flatten().buffer( size: Integer.MAX_VALUE, remainder: true ) From fd096713ae791538015a975edbc9eeade1606001 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Thu, 26 Sep 2024 11:18:17 +0200 Subject: [PATCH 41/61] Added more output channels to the top level workflow --- main.nf | 4 ++++ subworkflows/local/higher_level.nf | 6 +++++- workflows/rangeland.nf | 6 +++++- 3 files changed, 14 insertions(+), 2 deletions(-) diff --git a/main.nf b/main.nf index d1877fd..f67e3ea 100644 --- a/main.nf +++ b/main.nf @@ -40,6 +40,10 @@ workflow NFCORE_RANGELAND { RANGELAND () emit: + level2_ard = RANGELAND.out.level2_ard + mosaic = RANGELAND.out.mosaic + pyramid = RANGELAND.out.pyramid + trends = RANGELAND.out.trends multiqc_report = RANGELAND.out.multiqc_report // channel: /path/to/multiqc_report.html } diff --git a/subworkflows/local/higher_level.nf b/subworkflows/local/higher_level.nf index ecaa94e..2feda82 100644 --- a/subworkflows/local/higher_level.nf +++ b/subworkflows/local/higher_level.nf @@ -52,12 +52,16 @@ workflow HIGHER_LEVEL { ch_versions = ch_versions.mix(FORCE_MOSAIC.out.versions.first()) } + pyramid_files = Channel.empty() if (pyramid_visualization) { FORCE_PYRAMID( trend_files.filter { it[1].name.endsWith('.tif') }.map { [ it[1].simpleName.substring(0,11), it[1] ] } .groupTuple() ) + pyramid_files = FORCE_PYRAMID.out.trends ch_versions = ch_versions.mix(FORCE_PYRAMID.out.versions.first()) } emit: - mosaic_files + mosaic = mosaic_files + pyramid = pyramid_files + trends = FORCE_HIGHER_LEVEL.out.trend_files versions = ch_versions } diff --git a/workflows/rangeland.nf b/workflows/rangeland.nf index a83866e..469e641 100644 --- a/workflows/rangeland.nf +++ b/workflows/rangeland.nf @@ -147,7 +147,7 @@ workflow RANGELAND { ) ch_versions = ch_versions.mix(HIGHER_LEVEL.out.versions) - grouped_trend_data = HIGHER_LEVEL.out.mosaic_files.map{ it[1] }.flatten().buffer( size: Integer.MAX_VALUE, remainder: true ) + grouped_trend_data = HIGHER_LEVEL.out.mosaic.map{ it[1] }.flatten().buffer( size: Integer.MAX_VALUE, remainder: true ) // // MODULE: Check results @@ -226,6 +226,10 @@ workflow RANGELAND { ) emit: + level2_ard = PREPROCESSING.out.tiles_and_masks + mosaic = HIGHER_LEVEL.out.mosaic + pyramid = HIGHER_LEVEL.out.pyramid + trends = HIGHER_LEVEL.out.trends multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html versions = ch_versions // channel: [ path(versions.yml) ] } From a4d2e7fb699001a8b55974f9f23071fa0756a3e1 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Sat, 28 Sep 2024 09:07:49 +0200 Subject: [PATCH 42/61] Added automatic tarball extraction for input parameters --- conf/test.config | 4 --- conf/test_full.config | 4 --- docs/output.md | 5 +-- docs/usage.md | 20 ++++++----- nextflow.config | 4 --- nextflow_schema.json | 26 +++------------ workflows/rangeland.nf | 76 ++++++++++++++++++++++++++---------------- 7 files changed, 67 insertions(+), 72 deletions(-) diff --git a/conf/test.config b/conf/test.config index d8b1d47..1c59bed 100644 --- a/conf/test.config +++ b/conf/test.config @@ -24,10 +24,6 @@ params { dem = 'https://github.com/nf-core/test-datasets/raw/rangeland/dem/dem.tar.gz' wvdb = 'https://github.com/nf-core/test-datasets/raw/rangeland/wvp/wvdb.tar.gz' - input_tar = true - dem_tar = true - wvdb_tar = true - data_cube = 'https://github.com/nf-core/test-datasets/raw/rangeland/datacube/datacube-definition.prj' aoi = 'https://github.com/nf-core/test-datasets/raw/rangeland/vector/aoi.gpkg' endmember = 'https://github.com/nf-core/test-datasets/raw/rangeland/endmember/hostert-2003.txt' diff --git a/conf/test_full.config b/conf/test_full.config index b963c70..4146ca8 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -19,10 +19,6 @@ params { dem = 's3://ngi-igenomes/test-data/rangeland/dem.tar' wvdb = 's3://ngi-igenomes/test-data/rangeland/wvdb.tar' - input_tar = true - dem_tar = true - wvdb_tar = true - data_cube = 's3://ngi-igenomes/test-data/rangeland/datacube-definition.prj' aoi = 's3://ngi-igenomes/test-data/rangeland/aoi.gpkg' endmember = 's3://ngi-igenomes/test-data/rangeland/hostert-2003.txt' diff --git a/docs/output.md b/docs/output.md index 00ba296..cc7f24d 100644 --- a/docs/output.md +++ b/docs/output.md @@ -39,8 +39,9 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d [untar](https://nf-co.re/modules/untar) is a nf-core module used to extract files from tar archives. -Invocation of untar depends on certain parameters (i.e `--input_tar`, `--dem_tar` and `--wvdb_tar`). -Thus, the outputs files are only generated when these are set to `true`. +[untar](https://nf-co.re/modules/untar) is automatically executed when certain input parameters where given as `.tar` or `.tar.gz` files. +The parameters `--input`, `--dem` and `--wvdb` are supported. +See [Usage](usage.md) for details. ### Preparation diff --git a/docs/usage.md b/docs/usage.md index 0244faa..fba531c 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -106,9 +106,10 @@ The satellite imagery can be given to the pipeline using: --input '[path to imagery root]' ``` -The satellite imagery can also be provide as a tar archive. -In this case it is mandatory to set `--input_tar` to `true`. -Moreover, within the tar archive, the structure explained above has to be in place. +The satellite imagery can also be provide as a tarball (`.tar` or `.tar.gz` files). +These files will be automatically extracted. +Providing tarballs can be specifically helpful when using foreign files as inputs. +In this case, it is mandatory to have the structure explained above in place. In the example above `181036/` and `181035/` would need to be in the top level of the archive. ### Digital Elevation Model (DEM) @@ -134,9 +135,10 @@ The DEM can be given to the pipeline using: --dem '[path to dem root]' ``` -The digital elevation model can also be provide as a tar archive. -In this case it is mandatory to set `--dem_tar` to `true`. -Moreover, within the tar archive, the structure explained above has to be in place. +The digital elevation model can also be provide as a tarball (`.tar` or `.tar.gz` files). +These files will be automatically extracted. +Providing tarballs can be specifically helpful when using foreign files as inputs. +In this case, it is mandatory to have the structure explained above in place. In the example above `.vrt` and `/` would need to be in the top level of the archive. ### Water Vapor Database (WVDB) @@ -160,8 +162,10 @@ The WVDB can be given to the pipeline using: --wvdb '[path to wvdb dir]' ``` -The water vapor database can also be provide as a tar archive. -In this case it is mandatory to set `--wvdb_tar` to `true`. +The water vapor database can also be provide as a tarball (`.tar` or `.tar.gz` files). +These files will be automatically extracted. +Providing tarballs can be specifically helpful when using foreign files as inputs. +In this case, it is mandatory to have the structure explained above in place. All files of the wvdb would need to be in the top level of the archive. ### Datacube diff --git a/nextflow.config b/nextflow.config index 940ca41..dae1f35 100644 --- a/nextflow.config +++ b/nextflow.config @@ -17,10 +17,6 @@ params { aoi = null endmember = null - input_tar = false - dem_tar = false - wvdb_tar = false - // Remote sensing imagery parameters sensors_level2 = "LND04 LND05 LND07" resolution = 30 diff --git a/nextflow_schema.json b/nextflow_schema.json index c1f7d19..90772e3 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -15,43 +15,25 @@ "input": { "type": "string", "fa_icon": "fas fa-satellite", - "help_text": "Set this to the root directory of all satellite imagery. Directory structure should match format of data downloaded with '$ force-level1-csd\n' For concrete directory structure see [usage documentation](https://nf-co.re/rangeland/docs/usage)", - "description": "Root directory of all satellite imagery.", + "help_text": "Set this to the root directory of all satellite imagery. Directory structure should match format of data downloaded with '$ force-level1-csd\n' For concrete directory structure see [usage documentation](https://nf-co.re/rangeland/docs/usage).\n\nAlternatively, a tarball can be supplied. The same directory structure needs to be in place in that tarball.", + "description": "Root directory or tarball of all satellite imagery.", "mimetype": "application/x-tar", "format": "path" }, - "input_tar": { - "type": "boolean", - "fa_icon": "fas fa-archive", - "description": "Indicates whether `input` is a tar archive.", - "help_text": "Set to `true` if `input` is a tar archive. The workflow will extract it then." - }, "dem": { "type": "string", - "help_text": "Path to directory containing a subdirectory with tile-wise digital elevation files (`.tif`) and a virtual dataset file (`.vrt` file) comprising all tile-wise files from the subdirectory.", + "help_text": "Path to directory containing a subdirectory with tile-wise digital elevation files (`.tif`) and a virtual dataset file (`.vrt` file) comprising all tile-wise files from the subdirectory. See [usage documentation](https://nf-co.re/rangeland/docs/usage) for details regarding the required structure.\n\nAlternatively, a tarball can be supplied. The same directory structure needs to be in place in that tarball.\n\nAlternatively, a tarball can be supplied. The same directory structure needs to be in place in that tarball.", "fa_icon": "fas fa-mountain", "description": "Digital elevation model.", "format": "path" }, - "dem_tar": { - "type": "boolean", - "fa_icon": "fas fa-archive", - "description": "Indicates whether `dem` is a tar archive.", - "help_text": "Set to `true` if `dem` is a tar archive. The workflow will extract it then." - }, "wvdb": { "type": "string", "fa_icon": "fas fa-burn", "description": "Water vapor dataset.", - "help_text": "Directory containing a number text files describing global water vapor data at different timestamps, and a coordinate order (`.coo`-)file containing the reference system of the water vapor data.", + "help_text": "Directory containing a number text files describing global water vapor data at different timestamps, and a coordinate order (`.coo`-)file containing the reference system of the water vapor data. See [usage documentation](https://nf-co.re/rangeland/docs/usage) for details regarding the required structure.\n\nAlternatively, a tarball can be supplied. The same directory structure needs to be in place in that tarball.", "format": "path" }, - "wvdb_tar": { - "type": "boolean", - "fa_icon": "fas fa-archive", - "description": "Indicates whether `wvdb` is a tar archive.", - "help_text": "Set to `true` if `wvdb` is a tar archive. The workflow will extract it then." - }, "data_cube": { "type": "string", "description": "Datacube definition.", diff --git a/workflows/rangeland.nf b/workflows/rangeland.nf index 469e641..53149f1 100644 --- a/workflows/rangeland.nf +++ b/workflows/rangeland.nf @@ -67,14 +67,14 @@ workflow RANGELAND { main: - ch_versions = Channel.empty() + ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() // // Stage and validate input files // - data = null - dem = null - wvdb = null + data = Channel.empty() + dem = Channel.empty() + wvdb = Channel.empty() cube_file = file( params.data_cube ) aoi_file = file( params.aoi ) endmember_file = file( params.endmember ) @@ -83,37 +83,57 @@ workflow RANGELAND { // MODULE: untar // tar_versions = Channel.empty() - if (params.input_tar) { - UNTAR_INPUT([[:], params.input]) - base_path = UNTAR_INPUT.out.untar.map(it -> it[1]) - data = base_path.map(it -> file("$it/*/*", type: 'dir')).flatten() - data = data.filter{ inRegion(it) } - - tar_versions = tar_versions.mix(UNTAR_INPUT.out.versions) - } else { - data = Channel.fromPath( "${params.input}/*/*", type: 'dir').flatten() - data = data.filter{ inRegion(it) } + // Determine type of params.input and extract when neccessary + ch_input = Channel.of(file(params.input)) + ch_input.branch { it + archives : it.name.endsWith('tar') || it.name.endsWith('tar.gz') + return tuple([:], it) + dirs: true + return it } - - if (params.dem_tar) { - UNTAR_DEM([[:], params.dem]) - dem = UNTAR_DEM.out.untar.map(it -> file(it[1])) - - tar_versions = tar_versions.mix(UNTAR_DEM.out.versions) - } else { - dem = file("$params.dem") + .set{ ch_input_types } + + UNTAR_INPUT(ch_input_types.archives) + ch_untared_inputs = UNTAR_INPUT.out.untar.map(it -> it[1]) + tar_versions = tar_versions.mix(UNTAR_INPUT.out.versions) + + data = data + .mix(ch_untared_inputs, ch_input_types.dirs) + .map(it -> file("$it/*/*", type: 'dir')).flatten() + .filter{ inRegion(it) } + + // Determine type of params.dem and extract when neccessary + ch_dem = Channel.of(file(params.dem)) + ch_dem.branch { it + archives : it.name.endsWith('tar') || it.name.endsWith('tar.gz') + return tuple([:], it) + dirs: true + return file(it) } + .set{ ch_dem_types } - if (params.wvdb_tar) { - UNTAR_WVDB([[:], params.wvdb]) - wvdb = UNTAR_WVDB.out.untar.map(it -> file(it[1])) + UNTAR_DEM(ch_dem_types.archives) + ch_untared_dem = UNTAR_DEM.out.untar.map(it -> it[1]) + tar_versions = tar_versions.mix(UNTAR_DEM.out.versions) - tar_versions = tar_versions.mix(UNTAR_WVDB.out.versions) - } else { - wvdb = file("$params.wvdb") + dem = dem.mix(ch_untared_dem, ch_dem_types.dirs).first() + + // Determine type of params.wvdb and extract when neccessary + ch_wvdb = Channel.of(file(params.wvdb)) + ch_wvdb.branch { it + archives : it.name.endsWith('tar') || it.name.endsWith('tar.gz') + return tuple([:], it) + dirs: true + return file(it) } + .set{ ch_wvdb_types } + + UNTAR_WVDB(ch_wvdb_types.archives) + ch_untared_wvdb = UNTAR_WVDB.out.untar.map(it -> it[1]) + tar_versions = tar_versions.mix(UNTAR_WVDB.out.versions) + wvdb = wvdb.mix(ch_untared_wvdb, ch_wvdb_types.dirs).first() // // SUBWORKFLOW: Preprocess satellite imagery From 99ff90908c354890e570d3ad093ed1c450b1b4bd Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Mon, 7 Oct 2024 15:42:10 +0200 Subject: [PATCH 43/61] Changed UNTAR container to its default container image --- conf/test.config | 1 - conf/test_full.config | 1 - 2 files changed, 2 deletions(-) diff --git a/conf/test.config b/conf/test.config index 1c59bed..17e3213 100644 --- a/conf/test.config +++ b/conf/test.config @@ -55,7 +55,6 @@ params { process { withName: "UNTAR_*" { - container = 'docker.io/ubuntu:23.10' ext.args2 = "--strip-components=0" } } diff --git a/conf/test_full.config b/conf/test_full.config index 4146ca8..d6851b2 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -45,7 +45,6 @@ params { process { withName: "UNTAR_*" { - container = 'docker.io/ubuntu:23.10' ext.args2 = "--strip-components=1" } withName: "UNTAR_REF"{ From 605f15417ead5cdb7629bdeb103218f693002ce3 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Fri, 11 Oct 2024 09:19:30 +0200 Subject: [PATCH 44/61] Changed force-pyramid to process a single file instead of groups of files --- modules/local/force-pyramid/main.nf | 6 ++---- subworkflows/local/higher_level.nf | 2 +- 2 files changed, 3 insertions(+), 5 deletions(-) diff --git a/modules/local/force-pyramid/main.nf b/modules/local/force-pyramid/main.nf index 80d430c..34bac8b 100644 --- a/modules/local/force-pyramid/main.nf +++ b/modules/local/force-pyramid/main.nf @@ -16,10 +16,8 @@ process FORCE_PYRAMID { script: """ - files="*.tif" - for file in \$files; do - force-pyramid \$file - done; + file="*.tif" + force-pyramid \$file cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/subworkflows/local/higher_level.nf b/subworkflows/local/higher_level.nf index 2feda82..1891ae0 100644 --- a/subworkflows/local/higher_level.nf +++ b/subworkflows/local/higher_level.nf @@ -54,7 +54,7 @@ workflow HIGHER_LEVEL { pyramid_files = Channel.empty() if (pyramid_visualization) { - FORCE_PYRAMID( trend_files.filter { it[1].name.endsWith('.tif') }.map { [ it[1].simpleName.substring(0,11), it[1] ] } .groupTuple() ) + FORCE_PYRAMID( trend_files.filter { it[1].name.endsWith('.tif') }.map { [ it[1].simpleName.substring(0,11), it[1] ] } ) pyramid_files = FORCE_PYRAMID.out.trends ch_versions = ch_versions.mix(FORCE_PYRAMID.out.versions.first()) } From e8731e1f61b3e4d0e70550ba4d94f7af676e9fae Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Mon, 14 Oct 2024 16:29:53 +0200 Subject: [PATCH 45/61] Added missing description in the manifest scope. --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 4113199..89d56f8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -257,7 +257,7 @@ manifest { name = 'nf-core/rangeland' author = """Fabian Lehmann, David Frantz, Felix Kummer""" homePage = 'https://github.com/nf-core/rangeland' - description = """""" + description = """Long-term vegetation trend analysis pipeline for rangeland systems using satellite imagery.""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' version = '1.0.0' From 16b4a23c83cdd3c16b73b86ee533d788a7a1f12d Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 14 Oct 2024 14:44:27 +0000 Subject: [PATCH 46/61] Template update for nf-core/tools version 3.0.2 --- .github/CONTRIBUTING.md | 12 +- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/awsfulltest.yml | 25 +- .github/workflows/ci.yml | 57 ++- .github/workflows/download_pipeline.yml | 53 ++- .github/workflows/linting.yml | 23 +- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 2 +- .../workflows/template_version_comment.yml | 46 +++ .gitignore | 1 + .gitpod.yml | 7 +- .nf-core.yml | 20 +- .pre-commit-config.yaml | 2 +- CHANGELOG.md | 2 +- CITATIONS.md | 4 +- README.md | 5 +- assets/email_template.html | 2 +- assets/multiqc_config.yml | 4 +- assets/schema_input.json | 2 +- conf/base.config | 34 +- conf/modules.config | 1 - conf/test.config | 13 +- docs/images/mqc_fastqc_adapter.png | Bin 23458 -> 0 bytes docs/images/mqc_fastqc_counts.png | Bin 33918 -> 0 bytes docs/images/mqc_fastqc_quality.png | Bin 55769 -> 0 bytes docs/output.md | 10 - docs/usage.md | 12 +- main.nf | 8 - modules.json | 12 +- modules/nf-core/fastqc/environment.yml | 2 - modules/nf-core/fastqc/main.nf | 5 +- modules/nf-core/fastqc/meta.yml | 57 +-- modules/nf-core/fastqc/tests/main.nf.test | 225 ++++++++--- .../nf-core/fastqc/tests/main.nf.test.snap | 370 ++++++++++++++++-- modules/nf-core/multiqc/environment.yml | 4 +- modules/nf-core/multiqc/main.nf | 16 +- modules/nf-core/multiqc/meta.yml | 78 ++-- modules/nf-core/multiqc/tests/main.nf.test | 8 + .../nf-core/multiqc/tests/main.nf.test.snap | 24 +- modules/nf-core/multiqc/tests/nextflow.config | 5 + nextflow.config | 142 ++++--- nextflow_schema.json | 78 +--- .../utils_nfcore_rangeland_pipeline/main.nf | 65 ++- .../nf-core/utils_nextflow_pipeline/main.nf | 70 ++-- .../tests/nextflow.config | 2 +- .../nf-core/utils_nfcore_pipeline/main.nf | 306 ++++++++------- .../nf-core/utils_nfschema_plugin/main.nf | 46 +++ .../nf-core/utils_nfschema_plugin/meta.yml | 35 ++ .../utils_nfschema_plugin/tests/main.nf.test | 117 ++++++ .../tests/nextflow.config | 8 + .../tests/nextflow_schema.json | 8 +- .../nf-core/utils_nfvalidation_plugin/main.nf | 62 --- .../utils_nfvalidation_plugin/meta.yml | 44 --- .../tests/main.nf.test | 200 ---------- .../utils_nfvalidation_plugin/tests/tags.yml | 2 - workflows/rangeland.nf | 21 +- 56 files changed, 1395 insertions(+), 966 deletions(-) create mode 100644 .github/workflows/template_version_comment.yml delete mode 100755 docs/images/mqc_fastqc_adapter.png delete mode 100755 docs/images/mqc_fastqc_counts.png delete mode 100755 docs/images/mqc_fastqc_quality.png create mode 100644 modules/nf-core/multiqc/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config rename subworkflows/nf-core/{utils_nfvalidation_plugin => utils_nfschema_plugin}/tests/nextflow_schema.json (95%) delete mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf delete mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml delete mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test delete mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 18f544a..8225096 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -19,7 +19,7 @@ If you'd like to write some code for nf-core/rangeland, the standard workflow is 1. Check that there isn't already an issue about your idea in the [nf-core/rangeland issues](https://github.com/nf-core/rangeland/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this 2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/rangeland repository](https://github.com/nf-core/rangeland) to your GitHub account 3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions) -4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10). +4. Use `nf-core pipelines schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10). 5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged If you're not used to this workflow with git, you can start with some [docs from GitHub](https://help.github.com/en/github/collaborating-with-issues-and-pull-requests) or even their [excellent `git` resources](https://try.github.io/). @@ -40,7 +40,7 @@ There are typically two types of tests that run: ### Lint tests `nf-core` has a [set of guidelines](https://nf-co.re/developers/guidelines) which all pipelines must adhere to. -To enforce these and ensure that all pipelines stay in sync, we have developed a helper tool which runs checks on the pipeline code. This is in the [nf-core/tools repository](https://github.com/nf-core/tools) and once installed can be run locally with the `nf-core lint ` command. +To enforce these and ensure that all pipelines stay in sync, we have developed a helper tool which runs checks on the pipeline code. This is in the [nf-core/tools repository](https://github.com/nf-core/tools) and once installed can be run locally with the `nf-core pipelines lint ` command. If any failures or warnings are encountered, please follow the listed URL for more documentation. @@ -75,7 +75,7 @@ If you wish to contribute a new step, please use the following coding standards: 2. Write the process block (see below). 3. Define the output channel if needed (see below). 4. Add any new parameters to `nextflow.config` with a default (see below). -5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core schema build` tool). +5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core pipelines schema build` tool). 6. Add sanity checks and validation for all relevant parameters. 7. Perform local tests to validate that the new code works as expected. 8. If applicable, add a new test command in `.github/workflow/ci.yml`. @@ -86,11 +86,11 @@ If you wish to contribute a new step, please use the following coding standards: Parameters should be initialised / defined with default values in `nextflow.config` under the `params` scope. -Once there, use `nf-core schema build` to add to `nextflow_schema.json`. +Once there, use `nf-core pipelines schema build` to add to `nextflow_schema.json`. ### Default processes resource requirements -Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. +Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/main/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. @@ -103,7 +103,7 @@ Please use the following naming schemes, to make it easy to understand what is g ### Nextflow version bumping -If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core bump-version --nextflow . [min-nf-version]` +If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core pipelines bump-version --nextflow . [min-nf-version]` ### Images and figures diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 775ca31..58ec59b 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -17,7 +17,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rang - [ ] If you've fixed a bug or added code that should be tested, add tests! - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rangeland/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/rangeland _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. -- [ ] Make sure your code lints (`nf-core lint`). +- [ ] Make sure your code lints (`nf-core pipelines lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index c16cc45..1273346 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -1,18 +1,35 @@ name: nf-core AWS full size tests -# This workflow is triggered on published releases. +# This workflow is triggered on PRs opened against the master branch. # It can be additionally triggered manually with GitHub actions workflow dispatch button. # It runs the -profile 'test_full' on AWS batch on: - release: - types: [published] + pull_request: + branches: + - master workflow_dispatch: + pull_request_review: + types: [submitted] + jobs: run-platform: name: Run AWS full tests - if: github.repository == 'nf-core/rangeland' + # run only if the PR is approved by at least 2 reviewers and against the master branch or manually triggered + if: github.repository == 'nf-core/rangeland' && github.event.review.state == 'approved' && github.event.pull_request.base.ref == 'master' || github.event_name == 'workflow_dispatch' runs-on: ubuntu-latest steps: + - uses: octokit/request-action@v2.x + id: check_approvals + with: + route: GET /repos/${{ github.repository }}/pulls/${{ github.event.pull_request.number }}/reviews + env: + GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} + - id: test_variables + if: github.event_name != 'workflow_dispatch' + run: | + JSON_RESPONSE='${{ steps.check_approvals.outputs.data }}' + CURRENT_APPROVALS_COUNT=$(echo $JSON_RESPONSE | jq -c '[.[] | select(.state | contains("APPROVED")) ] | length') + test $CURRENT_APPROVALS_COUNT -ge 2 || exit 1 # At least 2 approvals are required - name: Launch workflow via Seqera Platform uses: seqeralabs/action-tower-launch@v2 # TODO nf-core: You can customise AWS full pipeline tests as required diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 7e6f66a..bded152 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -7,9 +7,12 @@ on: pull_request: release: types: [published] + workflow_dispatch: env: NXF_ANSI_LOG: false + NXF_SINGULARITY_CACHEDIR: ${{ github.workspace }}/.singularity + NXF_SINGULARITY_LIBRARYDIR: ${{ github.workspace }}/.singularity concurrency: group: "${{ github.workflow }}-${{ github.event.pull_request.number || github.ref }}" @@ -17,30 +20,66 @@ concurrency: jobs: test: - name: Run pipeline with test data + name: "Run pipeline with test data (${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }})" # Only run on push if this is the nf-core dev branch (merged PRs) if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/rangeland') }}" runs-on: ubuntu-latest strategy: matrix: NXF_VER: - - "23.04.0" + - "24.04.2" - "latest-everything" + profile: + - "conda" + - "docker" + - "singularity" + test_name: + - "test" + isMaster: + - ${{ github.base_ref == 'master' }} + # Exclude conda and singularity on dev + exclude: + - isMaster: false + profile: "conda" + - isMaster: false + profile: "singularity" steps: - name: Check out pipeline code uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - - name: Install Nextflow + - name: Set up Nextflow uses: nf-core/setup-nextflow@v2 with: version: "${{ matrix.NXF_VER }}" - - name: Disk space cleanup + - name: Set up Apptainer + if: matrix.profile == 'singularity' + uses: eWaterCycle/setup-apptainer@main + + - name: Set up Singularity + if: matrix.profile == 'singularity' + run: | + mkdir -p $NXF_SINGULARITY_CACHEDIR + mkdir -p $NXF_SINGULARITY_LIBRARYDIR + + - name: Set up Miniconda + if: matrix.profile == 'conda' + uses: conda-incubator/setup-miniconda@a4260408e20b96e80095f42ff7f1a15b27dd94ca # v3 + with: + miniconda-version: "latest" + auto-update-conda: true + conda-solver: libmamba + channels: conda-forge,bioconda + + - name: Set up Conda + if: matrix.profile == 'conda' + run: | + echo $(realpath $CONDA)/condabin >> $GITHUB_PATH + echo $(realpath python) >> $GITHUB_PATH + + - name: Clean up Disk space uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 - - name: Run pipeline with test data - # TODO nf-core: You can customise CI pipeline run tests as required - # For example: adding multiple test runs with different parameters - # Remember that you can parallelise this by using strategy.matrix + - name: "Run pipeline with test data ${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }}" run: | - nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results + nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.test_name }},${{ matrix.profile }} --outdir ./results diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 2d20d64..713dc3e 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -1,4 +1,4 @@ -name: Test successful pipeline download with 'nf-core download' +name: Test successful pipeline download with 'nf-core pipelines download' # Run the workflow when: # - dispatched manually @@ -8,7 +8,7 @@ on: workflow_dispatch: inputs: testbranch: - description: "The specific branch you wish to utilize for the test execution of nf-core download." + description: "The specific branch you wish to utilize for the test execution of nf-core pipelines download." required: true default: "dev" pull_request: @@ -39,9 +39,11 @@ jobs: with: python-version: "3.12" architecture: "x64" - - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 + + - name: Setup Apptainer + uses: eWaterCycle/setup-apptainer@4bb22c52d4f63406c49e94c804632975787312b3 # v2.0.0 with: - singularity-version: 3.8.3 + apptainer-version: 1.3.4 - name: Install dependencies run: | @@ -54,33 +56,64 @@ jobs: echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} + - name: Make a cache directory for the container images + run: | + mkdir -p ./singularity_container_images + - name: Download the pipeline env: - NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_CACHEDIR: ./singularity_container_images run: | - nf-core download ${{ env.REPO_LOWERCASE }} \ + nf-core pipelines download ${{ env.REPO_LOWERCASE }} \ --revision ${{ env.REPO_BRANCH }} \ --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ --compress "none" \ --container-system 'singularity' \ - --container-library "quay.io" -l "docker.io" -l "ghcr.io" \ + --container-library "quay.io" -l "docker.io" -l "community.wave.seqera.io" \ --container-cache-utilisation 'amend' \ - --download-configuration + --download-configuration 'yes' - name: Inspect download run: tree ./${{ env.REPOTITLE_LOWERCASE }} + - name: Count the downloaded number of container images + id: count_initial + run: | + image_count=$(ls -1 ./singularity_container_images | wc -l | xargs) + echo "Initial container image count: $image_count" + echo "IMAGE_COUNT_INITIAL=$image_count" >> ${GITHUB_ENV} + - name: Run the downloaded pipeline (stub) id: stub_run_pipeline continue-on-error: true env: - NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_CACHEDIR: ./singularity_container_images NXF_SINGULARITY_HOME_MOUNT: true run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results - name: Run the downloaded pipeline (stub run not supported) id: run_pipeline if: ${{ job.steps.stub_run_pipeline.status == failure() }} env: - NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_CACHEDIR: ./singularity_container_images NXF_SINGULARITY_HOME_MOUNT: true run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results + + - name: Count the downloaded number of container images + id: count_afterwards + run: | + image_count=$(ls -1 ./singularity_container_images | wc -l | xargs) + echo "Post-pipeline run container image count: $image_count" + echo "IMAGE_COUNT_AFTER=$image_count" >> ${GITHUB_ENV} + + - name: Compare container image counts + run: | + if [ "${{ env.IMAGE_COUNT_INITIAL }}" -ne "${{ env.IMAGE_COUNT_AFTER }}" ]; then + initial_count=${{ env.IMAGE_COUNT_INITIAL }} + final_count=${{ env.IMAGE_COUNT_AFTER }} + difference=$((final_count - initial_count)) + echo "$difference additional container images were \n downloaded at runtime . The pipeline has no support for offline runs!" + tree ./singularity_container_images + exit 1 + else + echo "The pipeline can be downloaded successfully!" + fi diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 1fcafe8..a502573 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -1,6 +1,6 @@ name: nf-core linting # This workflow is triggered on pushes and PRs to the repository. -# It runs the `nf-core lint` and markdown lint tests to ensure +# It runs the `nf-core pipelines lint` and markdown lint tests to ensure # that the code meets the nf-core guidelines. on: push: @@ -41,17 +41,32 @@ jobs: python-version: "3.12" architecture: "x64" + - name: read .nf-core.yml + uses: pietrobolcato/action-read-yaml@1.1.0 + id: read_yml + with: + config: ${{ github.workspace }}/.nf-core.yml + - name: Install dependencies run: | python -m pip install --upgrade pip - pip install nf-core + pip install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }} + + - name: Run nf-core pipelines lint + if: ${{ github.base_ref != 'master' }} + env: + GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }} + GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} + GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }} + run: nf-core -l lint_log.txt pipelines lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md - - name: Run nf-core lint + - name: Run nf-core pipelines lint --release + if: ${{ github.base_ref == 'master' }} env: GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }} GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }} - run: nf-core -l lint_log.txt lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md + run: nf-core -l lint_log.txt pipelines lint --release --dir ${GITHUB_WORKSPACE} --markdown lint_results.md - name: Save PR number if: ${{ always() }} diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 40acc23..42e519b 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3 + uses: dawidd6/action-download-artifact@bf251b5aa9c2f7eeb574a96ee720e24f801b7c11 # v6 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 03ecfcf..c6ba35d 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -12,7 +12,7 @@ jobs: - name: get topics and convert to hashtags id: get_topics run: | - echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')" >> $GITHUB_OUTPUT + echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')" | sed 's/-//g' >> $GITHUB_OUTPUT - uses: rzr/fediverse-action@master with: diff --git a/.github/workflows/template_version_comment.yml b/.github/workflows/template_version_comment.yml new file mode 100644 index 0000000..e8aafe4 --- /dev/null +++ b/.github/workflows/template_version_comment.yml @@ -0,0 +1,46 @@ +name: nf-core template version comment +# This workflow is triggered on PRs to check if the pipeline template version matches the latest nf-core version. +# It posts a comment to the PR, even if it comes from a fork. + +on: pull_request_target + +jobs: + template_version: + runs-on: ubuntu-latest + steps: + - name: Check out pipeline code + uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 + with: + ref: ${{ github.event.pull_request.head.sha }} + + - name: Read template version from .nf-core.yml + uses: nichmor/minimal-read-yaml@v0.0.2 + id: read_yml + with: + config: ${{ github.workspace }}/.nf-core.yml + + - name: Install nf-core + run: | + python -m pip install --upgrade pip + pip install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }} + + - name: Check nf-core outdated + id: nf_core_outdated + run: echo "OUTPUT=$(pip list --outdated | grep nf-core)" >> ${GITHUB_ENV} + + - name: Post nf-core template version comment + uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 + if: | + contains(env.OUTPUT, 'nf-core') + with: + repo-token: ${{ secrets.NF_CORE_BOT_AUTH_TOKEN }} + allow-repeats: false + message: | + > [!WARNING] + > Newer version of the nf-core template is available. + > + > Your pipeline is using an old version of the nf-core template: ${{ steps.read_yml.outputs['nf_core_version'] }}. + > Please update your pipeline to the latest version. + > + > For more documentation on how to update your pipeline, please see the [nf-core documentation](https://github.com/nf-core/tools?tab=readme-ov-file#sync-a-pipeline-with-the-template) and [Synchronisation documentation](https://nf-co.re/docs/contributing/sync). + # diff --git a/.gitignore b/.gitignore index 5124c9a..a42ce01 100644 --- a/.gitignore +++ b/.gitignore @@ -6,3 +6,4 @@ results/ testing/ testing* *.pyc +null/ diff --git a/.gitpod.yml b/.gitpod.yml index 105a182..4611863 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -4,17 +4,14 @@ tasks: command: | pre-commit install --install-hooks nextflow self-update - - name: unset JAVA_TOOL_OPTIONS - command: | - unset JAVA_TOOL_OPTIONS vscode: extensions: # based on nf-core.nf-core-extensionpack - - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code + #- esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar - mechatroner.rainbow-csv # Highlight columns in csv files in different colors - # - nextflow.nextflow # Nextflow syntax highlighting + - nextflow.nextflow # Nextflow syntax highlighting - oderwat.indent-rainbow # Highlight indentation level - streetsidesoftware.code-spell-checker # Spelling checker for source code - charliermarsh.ruff # Code linter Ruff diff --git a/.nf-core.yml b/.nf-core.yml index d9b4c86..9981b0d 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,6 +1,20 @@ -nf_core_version: 2.14.1 +bump_version: null +lint: + files_exist: + - conf/igenomes.config +nf_core_version: 3.0.2 +org_path: null repository_type: pipeline template: - prefix: nf-core - skip: + author: Fabian Lehmann, David Frantz, Felix Kummer + description: Long-term vegetation trend analysis pipeline for rangeland systems + using satellite imagery. + force: false + is_nfcore: true + name: rangeland + org: nf-core + outdir: . + skip_features: - igenomes + version: 1.0.0 +update: null diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 4dc0f1d..9e9f0e1 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -7,7 +7,7 @@ repos: - prettier@3.2.5 - repo: https://github.com/editorconfig-checker/editorconfig-checker.python - rev: "2.7.3" + rev: "3.0.3" hooks: - id: editorconfig-checker alias: ec diff --git a/CHANGELOG.md b/CHANGELOG.md index 534b0a6..69d332e 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.0dev - [date] +## v1.0.0 - [date] Initial release of nf-core/rangeland, created with the [nf-core](https://nf-co.re/) template. diff --git a/CITATIONS.md b/CITATIONS.md index 5372c15..9e3a2b5 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -12,11 +12,11 @@ - [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) - > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. +> Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. - [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) - > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. +> Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. ## Software packaging/containerisation tools diff --git a/README.md b/README.md index 2e9df7f..f76d338 100644 --- a/README.md +++ b/README.md @@ -9,7 +9,7 @@ [![GitHub Actions Linting Status](https://github.com/nf-core/rangeland/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rangeland/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rangeland/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) @@ -67,8 +67,7 @@ nextflow run nf-core/rangeland \ ``` > [!WARNING] -> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; -> see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). +> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files). For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rangeland/usage) and the [parameter documentation](https://nf-co.re/rangeland/parameters). diff --git a/assets/email_template.html b/assets/email_template.html index a566131..f719cb5 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -4,7 +4,7 @@ - + nf-core/rangeland Pipeline Report diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index d2ae102..13dd96a 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/rangeland + This report has been generated by the nf-core/rangeland analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-rangeland-methods-description": order: -1000 diff --git a/assets/schema_input.json b/assets/schema_input.json index 8c4e376..e46c2ee 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -1,5 +1,5 @@ { - "$schema": "http://json-schema.org/draft-07/schema", + "$schema": "https://json-schema.org/draft/2020-12/schema", "$id": "https://raw.githubusercontent.com/nf-core/rangeland/master/assets/schema_input.json", "title": "nf-core/rangeland pipeline - params.input schema", "description": "Schema for the file provided with params.input", diff --git a/conf/base.config b/conf/base.config index 76e4569..60219f7 100644 --- a/conf/base.config +++ b/conf/base.config @@ -11,9 +11,9 @@ process { // TODO nf-core: Check the defaults for all processes - cpus = { check_max( 1 * task.attempt, 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + cpus = { 1 * task.attempt } + memory = { 6.GB * task.attempt } + time = { 4.h * task.attempt } errorStrategy = { task.exitStatus in ((130..145) + 104) ? 'retry' : 'finish' } maxRetries = 1 @@ -27,30 +27,30 @@ process { // TODO nf-core: Customise requirements for specific processes. // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors withLabel:process_single { - cpus = { check_max( 1 , 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + cpus = { 1 } + memory = { 6.GB * task.attempt } + time = { 4.h * task.attempt } } withLabel:process_low { - cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 12.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + cpus = { 2 * task.attempt } + memory = { 12.GB * task.attempt } + time = { 4.h * task.attempt } } withLabel:process_medium { - cpus = { check_max( 6 * task.attempt, 'cpus' ) } - memory = { check_max( 36.GB * task.attempt, 'memory' ) } - time = { check_max( 8.h * task.attempt, 'time' ) } + cpus = { 6 * task.attempt } + memory = { 36.GB * task.attempt } + time = { 8.h * task.attempt } } withLabel:process_high { - cpus = { check_max( 12 * task.attempt, 'cpus' ) } - memory = { check_max( 72.GB * task.attempt, 'memory' ) } - time = { check_max( 16.h * task.attempt, 'time' ) } + cpus = { 12 * task.attempt } + memory = { 72.GB * task.attempt } + time = { 16.h * task.attempt } } withLabel:process_long { - time = { check_max( 20.h * task.attempt, 'time' ) } + time = { 20.h * task.attempt } } withLabel:process_high_memory { - memory = { check_max( 200.GB * task.attempt, 'memory' ) } + memory = { 200.GB * task.attempt } } withLabel:error_ignore { errorStrategy = 'ignore' diff --git a/conf/modules.config b/conf/modules.config index d203d2b..d266a38 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -21,7 +21,6 @@ process { withName: FASTQC { ext.args = '--quiet' } - withName: 'MULTIQC' { ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ diff --git a/conf/test.config b/conf/test.config index 2e8bff0..879ccb5 100644 --- a/conf/test.config +++ b/conf/test.config @@ -10,15 +10,18 @@ ---------------------------------------------------------------------------------------- */ +process { + resourceLimits = [ + cpus: 4, + memory: '15.GB', + time: '1.h' + ] +} + params { config_profile_name = 'Test profile' config_profile_description = 'Minimal test dataset to check pipeline function' - // Limit resources so that this can run on GitHub Actions - max_cpus = 2 - max_memory = '6.GB' - max_time = '6.h' - // Input data // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets // TODO nf-core: Give any required params for the test so that command line flags are not needed diff --git a/docs/images/mqc_fastqc_adapter.png b/docs/images/mqc_fastqc_adapter.png deleted file mode 100755 index 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zXwhtT%ei{n#FzEH|C;yZ>+$!u_x#*+`=L8{b9SH^9&27u3G_Gxqxe`L2UJtdxghk z&-wzDFvLvW{chK5u3{n6GSKKy!P&C6w^IFpbD0bcp^A{{2lcLh_DXj@ybtYvc^;(2 M)78&qol`;+0Fu7JivR!s diff --git a/docs/output.md b/docs/output.md index 7ec8f8f..4a89245 100644 --- a/docs/output.md +++ b/docs/output.md @@ -29,16 +29,6 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d [FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). -![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png) - -![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png) - -![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png) - -:::note -The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. -::: - ### MultiQC
    diff --git a/docs/usage.md b/docs/usage.md index bd88671..da18515 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -85,9 +85,9 @@ The above pipeline run specified with a params file in yaml format: nextflow run nf-core/rangeland -profile docker -params-file params.yaml ``` -with `params.yaml` containing: +with: -```yaml +```yaml title="params.yaml" input: './samplesheet.csv' outdir: './results/' genome: 'GRCh37' @@ -199,14 +199,6 @@ See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs). -## Azure Resource Requests - -To be used with the `azurebatch` profile by specifying the `-profile azurebatch`. -We recommend providing a compute `params.vm_type` of `Standard_D16_v3` VMs by default but these options can be changed if required. - -Note that the choice of VM size depends on your quota and the overall workload during the analysis. -For a thorough list, please refer the [Azure Sizes for virtual machines in Azure](https://docs.microsoft.com/en-us/azure/virtual-machines/sizes). - ## Running in the background Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished. diff --git a/main.nf b/main.nf index 9792f74..3650120 100644 --- a/main.nf +++ b/main.nf @@ -9,8 +9,6 @@ ---------------------------------------------------------------------------------------- */ -nextflow.enable.dsl = 2 - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS @@ -20,7 +18,6 @@ nextflow.enable.dsl = 2 include { RANGELAND } from './workflows/rangeland' include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_rangeland_pipeline' include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_rangeland_pipeline' - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ NAMED WORKFLOWS FOR PIPELINE @@ -43,10 +40,8 @@ workflow NFCORE_RANGELAND { RANGELAND ( samplesheet ) - emit: multiqc_report = RANGELAND.out.multiqc_report // channel: /path/to/multiqc_report.html - } /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -57,13 +52,11 @@ workflow NFCORE_RANGELAND { workflow { main: - // // SUBWORKFLOW: Run initialisation tasks // PIPELINE_INITIALISATION ( params.version, - params.help, params.validate_params, params.monochrome_logs, args, @@ -77,7 +70,6 @@ workflow { NFCORE_RANGELAND ( PIPELINE_INITIALISATION.out.samplesheet ) - // // SUBWORKFLOW: Run completion tasks // diff --git a/modules.json b/modules.json index 22f15e0..c4d4381 100644 --- a/modules.json +++ b/modules.json @@ -7,12 +7,12 @@ "nf-core": { "fastqc": { "branch": "master", - "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", + "git_sha": "cf17ca47590cc578dfb47db1c2a44ef86f89976d", "installed_by": ["modules"] } } @@ -21,17 +21,17 @@ "nf-core": { "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "git_sha": "3aa0aec1d52d492fe241919f0c6100ebf0074082", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", + "git_sha": "1b6b9a3338d011367137808b49b923515080e3ba", "installed_by": ["subworkflows"] }, - "utils_nfvalidation_plugin": { + "utils_nfschema_plugin": { "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "git_sha": "bbd5a41f4535a8defafe6080e00ea74c45f4f96c", "installed_by": ["subworkflows"] } } diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml index 1787b38..691d4c7 100644 --- a/modules/nf-core/fastqc/environment.yml +++ b/modules/nf-core/fastqc/environment.yml @@ -1,7 +1,5 @@ -name: fastqc channels: - conda-forge - bioconda - - defaults dependencies: - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index d79f1c8..d8989f4 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -26,7 +26,10 @@ process FASTQC { def rename_to = old_new_pairs*.join(' ').join(' ') def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') - def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') + // The total amount of allocated RAM by FastQC is equal to the number of threads defined (--threads) time the amount of RAM defined (--memory) + // https://github.com/s-andrews/FastQC/blob/1faeea0412093224d7f6a07f777fad60a5650795/fastqc#L211-L222 + // Dividing the task.memory by task.cpu allows to stick to requested amount of RAM in the label + def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') / task.cpus // FastQC memory value allowed range (100 - 10000) def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb) diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml index ee5507e..4827da7 100644 --- a/modules/nf-core/fastqc/meta.yml +++ b/modules/nf-core/fastqc/meta.yml @@ -16,35 +16,44 @@ tools: homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ licence: ["GPL-2.0-only"] + identifier: biotools:fastqc input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - html: - type: file - description: FastQC report - pattern: "*_{fastqc.html}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.html": + type: file + description: FastQC report + pattern: "*_{fastqc.html}" - zip: - type: file - description: FastQC report archive - pattern: "*_{fastqc.zip}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.zip": + type: file + description: FastQC report archive + pattern: "*_{fastqc.zip}" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@drpatelh" - "@grst" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 70edae4..e9d79a0 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -23,17 +23,14 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. - // looks like this:
    Mon 2 Oct 2023
    test.gz
    - // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("
    ") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_single") } + { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. + // looks like this:
    Mon 2 Oct 2023
    test.gz
    + // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("    ") }, + { assert snapshot(process.out.versions).match() } ) } } @@ -54,16 +51,14 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("") }, - { assert path(process.out.html[0][1][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_paired") } + { assert process.success }, + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + { assert snapshot(process.out.versions).match() } ) } } @@ -83,13 +78,11 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } + { assert process.success }, + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + { assert snapshot(process.out.versions).match() } ) } } @@ -109,13 +102,11 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_bam") } + { assert process.success }, + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + { assert snapshot(process.out.versions).match() } ) } } @@ -138,22 +129,20 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, - { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, - { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("") }, - { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert path(process.out.html[0][1][2]).text.contains("") }, - { assert path(process.out.html[0][1][3]).text.contains("") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } + { assert process.success }, + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, + { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, + { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + { assert path(process.out.html[0][1][2]).text.contains("") }, + { assert path(process.out.html[0][1][3]).text.contains("") }, + { assert snapshot(process.out.versions).match() } ) } } @@ -173,21 +162,18 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } + { assert process.success }, + { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + { assert snapshot(process.out.versions).match() } ) } } test("sarscov2 single-end [fastq] - stub") { - options "-stub" - + options "-stub" when { process { """ @@ -201,12 +187,123 @@ nextflow_process { then { assertAll ( - { assert process.success }, - { assert snapshot(process.out.html.collect { file(it[1]).getName() } + - process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match("fastqc_stub") } + { assert process.success }, + { assert snapshot(process.out).match() } ) } } + test("sarscov2 paired-end [fastq] - stub") { + + options "-stub" + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("sarscov2 interleaved [fastq] - stub") { + + options "-stub" + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("sarscov2 paired-end [bam] - stub") { + + options "-stub" + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("sarscov2 multiple [fastq] - stub") { + + options "-stub" + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("sarscov2 custom_prefix - stub") { + + options "-stub" + when { + process { + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 86f7c31..d5db309 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,88 +1,392 @@ { - "fastqc_versions_interleaved": { + "sarscov2 custom_prefix": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:40:07.293713" + "timestamp": "2024-07-22T11:02:16.374038" }, - "fastqc_stub": { + "sarscov2 single-end [fastq] - stub": { "content": [ - [ - "test.html", - "test.zip", - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": true + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "test", + "single_end": true + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "test", + "single_end": true + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T11:02:24.993809" + }, + "sarscov2 custom_prefix - stub": { + "content": [ + { + "0": [ + [ + { + "id": "mysample", + "single_end": true + }, + "mysample.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "mysample", + "single_end": true + }, + "mysample.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "mysample", + "single_end": true + }, + "mysample.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "mysample", + "single_end": true + }, + "mysample.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:31:01.425198" + "timestamp": "2024-07-22T11:03:10.93942" }, - "fastqc_versions_multiple": { + "sarscov2 interleaved [fastq]": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:40:55.797907" + "timestamp": "2024-07-22T11:01:42.355718" }, - "fastqc_versions_bam": { + "sarscov2 paired-end [bam]": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:40:26.795862" + "timestamp": "2024-07-22T11:01:53.276274" }, - "fastqc_versions_single": { + "sarscov2 multiple [fastq]": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:39:27.043675" + "timestamp": "2024-07-22T11:02:05.527626" }, - "fastqc_versions_paired": { + "sarscov2 paired-end [fastq]": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T11:01:31.188871" + }, + "sarscov2 paired-end [fastq] - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T11:02:34.273566" + }, + "sarscov2 multiple [fastq] - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:39:47.584191" + "timestamp": "2024-07-22T11:03:02.304411" }, - "fastqc_versions_custom_prefix": { + "sarscov2 single-end [fastq]": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T11:01:19.095607" + }, + "sarscov2 interleaved [fastq] - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T11:02:44.640184" + }, + "sarscov2 paired-end [bam] - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:41:14.576531" + "timestamp": "2024-07-22T11:02:53.550742" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index ca39fb6..6f5b867 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -1,7 +1,5 @@ -name: multiqc channels: - conda-forge - bioconda - - defaults dependencies: - - bioconda::multiqc=1.21 + - bioconda::multiqc=1.25.1 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 47ac352..cc0643e 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,14 +3,16 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : - 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.25.1--pyhdfd78af_0' : + 'biocontainers/multiqc:1.25.1--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" path(multiqc_config) path(extra_multiqc_config) path(multiqc_logo) + path(replace_names) + path(sample_names) output: path "*multiqc_report.html", emit: report @@ -23,16 +25,22 @@ process MULTIQC { script: def args = task.ext.args ?: '' + def prefix = task.ext.prefix ? "--filename ${task.ext.prefix}.html" : '' def config = multiqc_config ? "--config $multiqc_config" : '' def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' - def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' + def logo = multiqc_logo ? "--cl-config 'custom_logo: \"${multiqc_logo}\"'" : '' + def replace = replace_names ? "--replace-names ${replace_names}" : '' + def samples = sample_names ? "--sample-names ${sample_names}" : '' """ multiqc \\ --force \\ $args \\ $config \\ + $prefix \\ $extra_config \\ $logo \\ + $replace \\ + $samples \\ . cat <<-END_VERSIONS > versions.yml @@ -44,7 +52,7 @@ process MULTIQC { stub: """ mkdir multiqc_data - touch multiqc_plots + mkdir multiqc_plots touch multiqc_report.html cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index 45a9bc3..b16c187 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,5 +1,6 @@ name: multiqc -description: Aggregate results from bioinformatics analyses across many samples into a single report +description: Aggregate results from bioinformatics analyses across many samples into + a single report keywords: - QC - bioinformatics tools @@ -12,40 +13,59 @@ tools: homepage: https://multiqc.info/ documentation: https://multiqc.info/docs/ licence: ["GPL-3.0-or-later"] + identifier: biotools:multiqc input: - - multiqc_files: - type: file - description: | - List of reports / files recognised by MultiQC, for example the html and zip output of FastQC - - multiqc_config: - type: file - description: Optional config yml for MultiQC - pattern: "*.{yml,yaml}" - - extra_multiqc_config: - type: file - description: Second optional config yml for MultiQC. Will override common sections in multiqc_config. - pattern: "*.{yml,yaml}" - - multiqc_logo: - type: file - description: Optional logo file for MultiQC - pattern: "*.{png}" + - - multiqc_files: + type: file + description: | + List of reports / files recognised by MultiQC, for example the html and zip output of FastQC + - - multiqc_config: + type: file + description: Optional config yml for MultiQC + pattern: "*.{yml,yaml}" + - - extra_multiqc_config: + type: file + description: Second optional config yml for MultiQC. Will override common sections + in multiqc_config. + pattern: "*.{yml,yaml}" + - - multiqc_logo: + type: file + description: Optional logo file for MultiQC + pattern: "*.{png}" + - - replace_names: + type: file + description: | + Optional two-column sample renaming file. First column a set of + patterns, second column a set of corresponding replacements. Passed via + MultiQC's `--replace-names` option. + pattern: "*.{tsv}" + - - sample_names: + type: file + description: | + Optional TSV file with headers, passed to the MultiQC --sample_names + argument. + pattern: "*.{tsv}" output: - report: - type: file - description: MultiQC report file - pattern: "multiqc_report.html" + - "*multiqc_report.html": + type: file + description: MultiQC report file + pattern: "multiqc_report.html" - data: - type: directory - description: MultiQC data dir - pattern: "multiqc_data" + - "*_data": + type: directory + description: MultiQC data dir + pattern: "multiqc_data" - plots: - type: file - description: Plots created by MultiQC - pattern: "*_data" + - "*_plots": + type: file + description: Plots created by MultiQC + pattern: "*_data" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@abhi18av" - "@bunop" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index f1c4242..33316a7 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -8,6 +8,8 @@ nextflow_process { tag "modules_nfcore" tag "multiqc" + config "./nextflow.config" + test("sarscov2 single-end [fastqc]") { when { @@ -17,6 +19,8 @@ nextflow_process { input[1] = [] input[2] = [] input[3] = [] + input[4] = [] + input[5] = [] """ } } @@ -41,6 +45,8 @@ nextflow_process { input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) input[2] = [] input[3] = [] + input[4] = [] + input[5] = [] """ } } @@ -66,6 +72,8 @@ nextflow_process { input[1] = [] input[2] = [] input[3] = [] + input[4] = [] + input[5] = [] """ } } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index bfebd80..2fcbb5f 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -2,14 +2,14 @@ "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-02-29T08:48:55.657331" + "timestamp": "2024-10-02T17:51:46.317523" }, "multiqc_stub": { "content": [ @@ -17,25 +17,25 @@ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-02-29T08:49:49.071937" + "timestamp": "2024-10-02T17:52:20.680978" }, "multiqc_versions_config": { "content": [ [ - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-02-29T08:49:25.457567" + "timestamp": "2024-10-02T17:52:09.185842" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/tests/nextflow.config b/modules/nf-core/multiqc/tests/nextflow.config new file mode 100644 index 0000000..c537a6a --- /dev/null +++ b/modules/nf-core/multiqc/tests/nextflow.config @@ -0,0 +1,5 @@ +process { + withName: 'MULTIQC' { + ext.prefix = null + } +} diff --git a/nextflow.config b/nextflow.config index 94ab6c8..5d0e4ad 100644 --- a/nextflow.config +++ b/nextflow.config @@ -13,6 +13,8 @@ params { // Input options input = null + + // MultiQC options multiqc_config = null multiqc_title = null @@ -29,48 +31,27 @@ params { monochrome_logs = false hook_url = null help = false + help_full = false + show_hidden = false version = false pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/' // Config options config_profile_name = null config_profile_description = null + custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null - // Max resource options - // Defaults only, expecting to be overwritten - max_memory = '128.GB' - max_cpus = 16 - max_time = '240.h' - // Schema validation default options - validationFailUnrecognisedParams = false - validationLenientMode = false - validationSchemaIgnoreParams = 'genomes,igenomes_base' - validationShowHiddenParams = false - validate_params = true - + validate_params = true } // Load base.config by default for all pipelines includeConfig 'conf/base.config' -// Load nf-core custom profiles from different Institutions -try { - includeConfig "${params.custom_config_base}/nfcore_custom.config" -} catch (Exception e) { - System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") -} - -// Load nf-core/rangeland custom profiles from different institutions. -try { - includeConfig "${params.custom_config_base}/pipeline/rangeland.config" -} catch (Exception e) { - System.err.println("WARNING: Could not load nf-core/config/rangeland profiles: ${params.custom_config_base}/pipeline/rangeland.config") -} profiles { debug { dumpHashes = true @@ -85,7 +66,7 @@ profiles { podman.enabled = false shifter.enabled = false charliecloud.enabled = false - conda.channels = ['conda-forge', 'bioconda', 'defaults'] + conda.channels = ['conda-forge', 'bioconda'] apptainer.enabled = false } mamba { @@ -174,18 +155,23 @@ profiles { test_full { includeConfig 'conf/test_full.config' } } -// Set default registry for Apptainer, Docker, Podman and Singularity independent of -profile -// Will not be used unless Apptainer / Docker / Podman / Singularity are enabled +// Load nf-core custom profiles from different Institutions +includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/nfcore_custom.config" : "/dev/null" + +// Load nf-core/rangeland custom profiles from different institutions. +// TODO nf-core: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs +// includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/rangeland.config" : "/dev/null" + +// Set default registry for Apptainer, Docker, Podman, Charliecloud and Singularity independent of -profile +// Will not be used unless Apptainer / Docker / Podman / Charliecloud / Singularity are enabled // Set to your registry if you have a mirror of containers -apptainer.registry = 'quay.io' -docker.registry = 'quay.io' -podman.registry = 'quay.io' -singularity.registry = 'quay.io' +apptainer.registry = 'quay.io' +docker.registry = 'quay.io' +podman.registry = 'quay.io' +singularity.registry = 'quay.io' +charliecloud.registry = 'quay.io' + -// Nextflow plugins -plugins { - id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet -} // Export these variables to prevent local Python/R libraries from conflicting with those in the container // The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container. @@ -198,8 +184,15 @@ env { JULIA_DEPOT_PATH = "/usr/local/share/julia" } -// Capture exit codes from upstream processes when piping -process.shell = ['/bin/bash', '-euo', 'pipefail'] +// Set bash options +process.shell = """\ +bash + +set -e # Exit if a tool returns a non-zero status/exit code +set -u # Treat unset variables and parameters as an error +set -o pipefail # Returns the status of the last command to exit with a non-zero status or zero if all successfully execute +set -C # No clobber - prevent output redirection from overwriting files. +""" // Disable process selector warnings by default. Use debug profile to enable warnings. nextflow.enable.configProcessNamesValidation = false @@ -226,45 +219,48 @@ manifest { name = 'nf-core/rangeland' author = """Fabian Lehmann, David Frantz, Felix Kummer""" homePage = 'https://github.com/nf-core/rangeland' - description = """""" + description = """Long-term vegetation trend analysis pipeline for rangeland systems using satellite imagery.""" mainScript = 'main.nf' - nextflowVersion = '!>=23.04.0' - version = '1.0dev' + nextflowVersion = '!>=24.04.2' + version = '1.0.0' doi = '' } -// Load modules.config for DSL2 module specific options -includeConfig 'conf/modules.config' +// Nextflow plugins +plugins { + id 'nf-schema@2.1.1' // Validation of pipeline parameters and creation of an input channel from a sample sheet +} -// Function to ensure that resource requirements don't go beyond -// a maximum limit -def check_max(obj, type) { - if (type == 'memory') { - try { - if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) - return params.max_memory as nextflow.util.MemoryUnit - else - return obj - } catch (all) { - println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'time') { - try { - if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) - return params.max_time as nextflow.util.Duration - else - return obj - } catch (all) { - println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'cpus') { - try { - return Math.min( obj, params.max_cpus as int ) - } catch (all) { - println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj" - return obj - } +validation { + defaultIgnoreParams = ["genomes"] + help { + enabled = true + command = "nextflow run $manifest.name -profile --input samplesheet.csv --outdir " + fullParameter = "help_full" + showHiddenParameter = "show_hidden" + beforeText = """ +-\033[2m----------------------------------------------------\033[0m- + \033[0;32m,--.\033[0;30m/\033[0;32m,-.\033[0m +\033[0;34m ___ __ __ __ ___ \033[0;32m/,-._.--~\'\033[0m +\033[0;34m |\\ | |__ __ / ` / \\ |__) |__ \033[0;33m} {\033[0m +\033[0;34m | \\| | \\__, \\__/ | \\ |___ \033[0;32m\\`-._,-`-,\033[0m + \033[0;32m`._,._,\'\033[0m +\033[0;35m ${manifest.name} ${manifest.version}\033[0m +-\033[2m----------------------------------------------------\033[0m- +""" + afterText = """${manifest.doi ? "* The pipeline\n" : ""}${manifest.doi.tokenize(",").collect { " https://doi.org/${it.trim().replace('https://doi.org/','')}"}.join("\n")}${manifest.doi ? "\n" : ""} +* The nf-core framework + https://doi.org/10.1038/s41587-020-0439-x + +* Software dependencies + https://github.com/${manifest.name}/blob/master/CITATIONS.md +""" + } + summary { + beforeText = validation.help.beforeText + afterText = validation.help.afterText } } + +// Load modules.config for DSL2 module specific options +includeConfig 'conf/modules.config' diff --git a/nextflow_schema.json b/nextflow_schema.json index 0dc3e47..07f5b59 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,10 +1,10 @@ { - "$schema": "http://json-schema.org/draft-07/schema", + "$schema": "https://json-schema.org/draft/2020-12/schema", "$id": "https://raw.githubusercontent.com/nf-core/rangeland/master/nextflow_schema.json", "title": "nf-core/rangeland pipeline parameters", - "description": "", + "description": "Long-term vegetation trend analysis pipeline for rangeland systems using satellite imagery.", "type": "object", - "definitions": { + "$defs": { "input_output_options": { "title": "Input/output options", "type": "object", @@ -91,41 +91,6 @@ } } }, - "max_job_request_options": { - "title": "Max job request options", - "type": "object", - "fa_icon": "fab fa-acquisitions-incorporated", - "description": "Set the top limit for requested resources for any single job.", - "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", - "properties": { - "max_cpus": { - "type": "integer", - "description": "Maximum number of CPUs that can be requested for any single job.", - "default": 16, - "fa_icon": "fas fa-microchip", - "hidden": true, - "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" - }, - "max_memory": { - "type": "string", - "description": "Maximum amount of memory that can be requested for any single job.", - "default": "128.GB", - "fa_icon": "fas fa-memory", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "hidden": true, - "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" - }, - "max_time": { - "type": "string", - "description": "Maximum amount of time that can be requested for any single job.", - "default": "240.h", - "fa_icon": "far fa-clock", - "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$", - "hidden": true, - "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" - } - } - }, "generic_options": { "title": "Generic options", "type": "object", @@ -133,12 +98,6 @@ "description": "Less common options for the pipeline, typically set in a config file.", "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", "properties": { - "help": { - "type": "boolean", - "description": "Display help text.", - "fa_icon": "fas fa-question-circle", - "hidden": true - }, "version": { "type": "boolean", "description": "Display version and exit.", @@ -214,27 +173,6 @@ "fa_icon": "fas fa-check-square", "hidden": true }, - "validationShowHiddenParams": { - "type": "boolean", - "fa_icon": "far fa-eye-slash", - "description": "Show all params when using `--help`", - "hidden": true, - "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." - }, - "validationFailUnrecognisedParams": { - "type": "boolean", - "fa_icon": "far fa-check-circle", - "description": "Validation of parameters fails when an unrecognised parameter is found.", - "hidden": true, - "help_text": "By default, when an unrecognised parameter is found, it returns a warinig." - }, - "validationLenientMode": { - "type": "boolean", - "fa_icon": "far fa-check-circle", - "description": "Validation of parameters in lenient more.", - "hidden": true, - "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." - }, "pipelines_testdata_base_path": { "type": "string", "fa_icon": "far fa-check-circle", @@ -247,16 +185,14 @@ }, "allOf": [ { - "$ref": "#/definitions/input_output_options" - }, - { - "$ref": "#/definitions/institutional_config_options" + "$ref": "#/$defs/input_output_options" }, + { - "$ref": "#/definitions/max_job_request_options" + "$ref": "#/$defs/institutional_config_options" }, { - "$ref": "#/definitions/generic_options" + "$ref": "#/$defs/generic_options" } ] } diff --git a/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf b/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf index 4dcd0fe..363f0e6 100644 --- a/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf @@ -8,29 +8,25 @@ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' -include { paramsSummaryMap } from 'plugin/nf-validation' -include { fromSamplesheet } from 'plugin/nf-validation' -include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { UTILS_NFSCHEMA_PLUGIN } from '../../nf-core/utils_nfschema_plugin' +include { paramsSummaryMap } from 'plugin/nf-schema' +include { samplesheetToList } from 'plugin/nf-schema' include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' -include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' -include { nfCoreLogo } from '../../nf-core/utils_nfcore_pipeline' include { imNotification } from '../../nf-core/utils_nfcore_pipeline' include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' -include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ SUBWORKFLOW TO INITIALISE PIPELINE -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow PIPELINE_INITIALISATION { take: version // boolean: Display version and exit - help // boolean: Display help text validate_params // boolean: Boolean whether to validate parameters against the schema at runtime monochrome_logs // boolean: Do not use coloured log outputs nextflow_cli_args // array: List of positional nextflow CLI args @@ -54,16 +50,10 @@ workflow PIPELINE_INITIALISATION { // // Validate parameters and generate parameter summary to stdout // - pre_help_text = nfCoreLogo(monochrome_logs) - post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) - def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input samplesheet.csv --outdir " - UTILS_NFVALIDATION_PLUGIN ( - help, - workflow_command, - pre_help_text, - post_help_text, + UTILS_NFSCHEMA_PLUGIN ( + workflow, validate_params, - "nextflow_schema.json" + null ) // @@ -76,8 +66,9 @@ workflow PIPELINE_INITIALISATION { // // Create channel from input file provided through params.input // + Channel - .fromSamplesheet("input") + .fromList(samplesheetToList(params.input, "${projectDir}/assets/schema_input.json")) .map { meta, fastq_1, fastq_2 -> if (!fastq_2) { @@ -87,8 +78,8 @@ workflow PIPELINE_INITIALISATION { } } .groupTuple() - .map { - validateInputSamplesheet(it) + .map { samplesheet -> + validateInputSamplesheet(samplesheet) } .map { meta, fastqs -> @@ -102,9 +93,9 @@ workflow PIPELINE_INITIALISATION { } /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ SUBWORKFLOW FOR PIPELINE COMPLETION -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow PIPELINE_COMPLETION { @@ -119,7 +110,6 @@ workflow PIPELINE_COMPLETION { multiqc_report // string: Path to MultiQC report main: - summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") // @@ -127,11 +117,18 @@ workflow PIPELINE_COMPLETION { // workflow.onComplete { if (email || email_on_fail) { - completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + completionEmail( + summary_params, + email, + email_on_fail, + plaintext_email, + outdir, + monochrome_logs, + multiqc_report.toList() + ) } completionSummary(monochrome_logs) - if (hook_url) { imNotification(summary_params, hook_url) } @@ -143,9 +140,9 @@ workflow PIPELINE_COMPLETION { } /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ FUNCTIONS -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ // @@ -155,14 +152,13 @@ def validateInputSamplesheet(input) { def (metas, fastqs) = input[1..2] // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end - def endedness_ok = metas.collect{ it.single_end }.unique().size == 1 + def endedness_ok = metas.collect{ meta -> meta.single_end }.unique().size == 1 if (!endedness_ok) { error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") } return [ metas[0], fastqs ] } - // // Generate methods description for MultiQC // @@ -204,8 +200,10 @@ def methodsDescriptionText(mqc_methods_yaml) { // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers // Removing ` ` since the manifest.doi is a string and not a proper list def temp_doi_ref = "" - String[] manifest_doi = meta.manifest_map.doi.tokenize(",") - for (String doi_ref: manifest_doi) temp_doi_ref += "(doi: ${doi_ref.replace("https://doi.org/", "").replace(" ", "")}), " + def manifest_doi = meta.manifest_map.doi.tokenize(",") + manifest_doi.each { doi_ref -> + temp_doi_ref += "(doi: ${doi_ref.replace("https://doi.org/", "").replace(" ", "")}), " + } meta["doi_text"] = temp_doi_ref.substring(0, temp_doi_ref.length() - 2) } else meta["doi_text"] = "" meta["nodoi_text"] = meta.manifest_map.doi ? "" : "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " @@ -226,3 +224,4 @@ def methodsDescriptionText(mqc_methods_yaml) { return description_html.toString() } + diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf index ac31f28..0fcbf7b 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -2,18 +2,13 @@ // Subworkflow with functionality that may be useful for any Nextflow pipeline // -import org.yaml.snakeyaml.Yaml -import groovy.json.JsonOutput -import nextflow.extension.FilesEx - /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ SUBWORKFLOW DEFINITION -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow UTILS_NEXTFLOW_PIPELINE { - take: print_version // boolean: print version dump_parameters // boolean: dump parameters @@ -26,7 +21,7 @@ workflow UTILS_NEXTFLOW_PIPELINE { // Print workflow version and exit on --version // if (print_version) { - log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + log.info("${workflow.manifest.name} ${getWorkflowVersion()}") System.exit(0) } @@ -49,16 +44,16 @@ workflow UTILS_NEXTFLOW_PIPELINE { } /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ FUNCTIONS -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ // // Generate version string // def getWorkflowVersion() { - String version_string = "" + def version_string = "" as String if (workflow.manifest.version) { def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' version_string += "${prefix_v}${workflow.manifest.version}" @@ -76,13 +71,13 @@ def getWorkflowVersion() { // Dump pipeline parameters to a JSON file // def dumpParametersToJSON(outdir) { - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def filename = "params_${timestamp}.json" - def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = JsonOutput.toJson(params) - temp_pf.text = JsonOutput.prettyPrint(jsonStr) + def timestamp = new java.util.Date().format('yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = groovy.json.JsonOutput.toJson(params) + temp_pf.text = groovy.json.JsonOutput.prettyPrint(jsonStr) - FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + nextflow.extension.FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") temp_pf.delete() } @@ -90,37 +85,40 @@ def dumpParametersToJSON(outdir) { // When running with -profile conda, warn if channels have not been set-up appropriately // def checkCondaChannels() { - Yaml parser = new Yaml() + def parser = new org.yaml.snakeyaml.Yaml() def channels = [] try { def config = parser.load("conda config --show channels".execute().text) channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return + } + catch (NullPointerException e) { + log.warn("Could not verify conda channel configuration.") + return null + } + catch (IOException e) { + log.warn("Could not verify conda channel configuration.") + return null } // Check that all channels are present // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def required_channels_in_order = ['conda-forge', 'bioconda'] def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean // Check that they are in the right order - def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) - } + def channel_priority_violation = required_channels_in_order != channels.findAll { ch -> ch in required_channels_in_order } if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + log.warn """\ + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + There is a problem with your Conda configuration! + You will need to set-up the conda-forge and bioconda channels correctly. + Please refer to https://bioconda.github.io/ + The observed channel order is + ${channels} + but the following channel order is required: + ${required_channels_in_order} + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + """.stripIndent(true) } } diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config index d0a926b..a09572e 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -3,7 +3,7 @@ manifest { author = """nf-core""" homePage = 'https://127.0.0.1' description = """Dummy pipeline""" - nextflowVersion = '!>=23.04.0' + nextflowVersion = '!>=23.04.0' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' } diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf index 14558c3..5cb7baf 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -2,17 +2,13 @@ // Subworkflow with utility functions specific to the nf-core pipeline template // -import org.yaml.snakeyaml.Yaml -import nextflow.extension.FilesEx - /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ SUBWORKFLOW DEFINITION -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow UTILS_NFCORE_PIPELINE { - take: nextflow_cli_args @@ -25,23 +21,20 @@ workflow UTILS_NFCORE_PIPELINE { } /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ FUNCTIONS -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ // // Warn if a -profile or Nextflow config has not been provided to run the pipeline // def checkConfigProvided() { - valid_config = true + def valid_config = true as Boolean if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " + log.warn( + "[${workflow.manifest.name}] You are attempting to run the pipeline without any custom configuration!\n\n" + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + "Please refer to the quick start section and usage docs for the pipeline.\n " + ) valid_config = false } return valid_config @@ -52,12 +45,14 @@ def checkConfigProvided() { // def checkProfileProvided(nextflow_cli_args) { if (workflow.profile.endsWith(',')) { - error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + - "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + error( + "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + ) } if (nextflow_cli_args[0]) { - log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + - "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + log.warn( + "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + ) } } @@ -66,25 +61,21 @@ def checkProfileProvided(nextflow_cli_args) { // def workflowCitation() { def temp_doi_ref = "" - String[] manifest_doi = workflow.manifest.doi.tokenize(",") - // Using a loop to handle multiple DOIs + def manifest_doi = workflow.manifest.doi.tokenize(",") + // Handling multiple DOIs // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers // Removing ` ` since the manifest.doi is a string and not a proper list - for (String doi_ref: manifest_doi) temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - "* The pipeline\n" + - temp_doi_ref + "\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + manifest_doi.each { doi_ref -> + temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" + } + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + "* The pipeline\n" + temp_doi_ref + "\n" + "* The nf-core framework\n" + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" } // // Generate workflow version string // def getWorkflowVersion() { - String version_string = "" + def version_string = "" as String if (workflow.manifest.version) { def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' version_string += "${prefix_v}${workflow.manifest.version}" @@ -102,8 +93,8 @@ def getWorkflowVersion() { // Get software versions for pipeline // def processVersionsFromYAML(yaml_file) { - Yaml yaml = new Yaml() - versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + def yaml = new org.yaml.snakeyaml.Yaml() + def versions = yaml.load(yaml_file).collectEntries { k, v -> [k.tokenize(':')[-1], v] } return yaml.dumpAsMap(versions).trim() } @@ -113,8 +104,8 @@ def processVersionsFromYAML(yaml_file) { def workflowVersionToYAML() { return """ Workflow: - $workflow.manifest.name: ${getWorkflowVersion()} - Nextflow: $workflow.nextflow.version + ${workflow.manifest.name}: ${getWorkflowVersion()} + Nextflow: ${workflow.nextflow.version} """.stripIndent().trim() } @@ -122,11 +113,7 @@ def workflowVersionToYAML() { // Get channel of software versions used in pipeline in YAML format // def softwareVersionsToYAML(ch_versions) { - return ch_versions - .unique() - .map { processVersionsFromYAML(it) } - .unique() - .mix(Channel.of(workflowVersionToYAML())) + return ch_versions.unique().map { version -> processVersionsFromYAML(version) }.unique().mix(Channel.of(workflowVersionToYAML())) } // @@ -134,25 +121,31 @@ def softwareVersionsToYAML(ch_versions) { // def paramsSummaryMultiqc(summary_params) { def summary_section = '' - for (group in summary_params.keySet()) { - def group_params = summary_params.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

    $group

    \n" - summary_section += "
    \n" - for (param in group_params.keySet()) { - summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + summary_params + .keySet() + .each { group -> + def group_params = summary_params.get(group) + // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    ${group}

    \n" + summary_section += "
    \n" + group_params + .keySet() + .sort() + .each { param -> + summary_section += "
    ${param}
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" } - summary_section += "
    \n" } - } - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" + def yaml_file_text = "id: '${workflow.manifest.name.replace('/', '-')}-summary'\n" as String + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" return yaml_file_text } @@ -161,7 +154,7 @@ def paramsSummaryMultiqc(summary_params) { // nf-core logo // def nfCoreLogo(monochrome_logs=true) { - Map colors = logColours(monochrome_logs) + def colors = logColours(monochrome_logs) as Map String.format( """\n ${dashedLine(monochrome_logs)} @@ -180,7 +173,7 @@ def nfCoreLogo(monochrome_logs=true) { // Return dashed line // def dashedLine(monochrome_logs=true) { - Map colors = logColours(monochrome_logs) + def colors = logColours(monochrome_logs) as Map return "-${colors.dim}----------------------------------------------------${colors.reset}-" } @@ -188,7 +181,7 @@ def dashedLine(monochrome_logs=true) { // ANSII colours used for terminal logging // def logColours(monochrome_logs=true) { - Map colorcodes = [:] + def colorcodes = [:] as Map // Reset / Meta colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" @@ -200,54 +193,54 @@ def logColours(monochrome_logs=true) { colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" return colorcodes } @@ -262,14 +255,15 @@ def attachMultiqcReport(multiqc_report) { mqc_report = multiqc_report.getVal() if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + log.warn("[${workflow.manifest.name}] Found multiple reports from process 'MULTIQC', will use only one") } mqc_report = mqc_report[0] } } - } catch (all) { + } + catch (Exception all) { if (multiqc_report) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + log.warn("[${workflow.manifest.name}] Could not attach MultiQC report to summary email") } } return mqc_report @@ -281,26 +275,35 @@ def attachMultiqcReport(multiqc_report) { def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + def subject = "[${workflow.manifest.name}] Successful: ${workflow.runName}" if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + subject = "[${workflow.manifest.name}] FAILED: ${workflow.runName}" } def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } + summary_params + .keySet() + .sort() + .each { group -> + summary << summary_params[group] + } def misc_fields = [:] misc_fields['Date Started'] = workflow.start misc_fields['Date Completed'] = workflow.complete misc_fields['Pipeline script file path'] = workflow.scriptFile misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build + if (workflow.repository) { + misc_fields['Pipeline repository Git URL'] = workflow.repository + } + if (workflow.commitId) { + misc_fields['Pipeline repository Git Commit'] = workflow.commitId + } + if (workflow.revision) { + misc_fields['Pipeline Git branch/tag'] = workflow.revision + } + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp def email_fields = [:] @@ -338,39 +341,41 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi // Render the sendmail template def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def smail_fields = [email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes()] def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") def sendmail_template = engine.createTemplate(sf).make(smail_fields) def sendmail_html = sendmail_template.toString() // Send the HTML e-mail - Map colors = logColours(monochrome_logs) + def colors = logColours(monochrome_logs) as Map if (email_address) { try { - if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + if (plaintext_email) { +new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML') } // Try to send HTML e-mail using sendmail def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") sendmail_tf.withWriter { w -> w << sendmail_html } - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { + ['sendmail', '-t'].execute() << sendmail_html + log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Sent summary e-mail to ${email_address} (sendmail)-") + } + catch (Exception all) { // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + def mail_cmd = ['mail', '-s', subject, '--content-type=text/html', email_address] mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Sent summary e-mail to ${email_address} (mail)-") } } // Write summary e-mail HTML to a file def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") output_hf.withWriter { w -> w << email_html } - FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + nextflow.extension.FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html") output_hf.delete() // Write summary e-mail TXT to a file def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") output_tf.withWriter { w -> w << email_txt } - FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + nextflow.extension.FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt") output_tf.delete() } @@ -378,15 +383,17 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi // Print pipeline summary on completion // def completionSummary(monochrome_logs=true) { - Map colors = logColours(monochrome_logs) + def colors = logColours(monochrome_logs) as Map if (workflow.success) { if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Pipeline completed successfully${colors.reset}-") + } + else { + log.info("-${colors.purple}[${workflow.manifest.name}]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-") } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + else { + log.info("-${colors.purple}[${workflow.manifest.name}]${colors.red} Pipeline completed with errors${colors.reset}-") } } @@ -395,21 +402,30 @@ def completionSummary(monochrome_logs=true) { // def imNotification(summary_params, hook_url) { def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } + summary_params + .keySet() + .sort() + .each { group -> + summary << summary_params[group] + } def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) { + misc_fields['repository'] = workflow.repository + } + if (workflow.commitId) { + misc_fields['commitid'] = workflow.commitId + } + if (workflow.revision) { + misc_fields['revision'] = workflow.revision + } + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp def msg_fields = [:] msg_fields['version'] = getWorkflowVersion() @@ -434,13 +450,13 @@ def imNotification(summary_params, hook_url) { def json_message = json_template.toString() // POST - def post = new URL(hook_url).openConnection(); + def post = new URL(hook_url).openConnection() post.setRequestMethod("POST") post.setDoOutput(true) post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); + post.getOutputStream().write(json_message.getBytes("UTF-8")) + def postRC = post.getResponseCode() + if (!postRC.equals(200)) { + log.warn(post.getErrorStream().getText()) } } diff --git a/subworkflows/nf-core/utils_nfschema_plugin/main.nf b/subworkflows/nf-core/utils_nfschema_plugin/main.nf new file mode 100644 index 0000000..4994303 --- /dev/null +++ b/subworkflows/nf-core/utils_nfschema_plugin/main.nf @@ -0,0 +1,46 @@ +// +// Subworkflow that uses the nf-schema plugin to validate parameters and render the parameter summary +// + +include { paramsSummaryLog } from 'plugin/nf-schema' +include { validateParameters } from 'plugin/nf-schema' + +workflow UTILS_NFSCHEMA_PLUGIN { + + take: + input_workflow // workflow: the workflow object used by nf-schema to get metadata from the workflow + validate_params // boolean: validate the parameters + parameters_schema // string: path to the parameters JSON schema. + // this has to be the same as the schema given to `validation.parametersSchema` + // when this input is empty it will automatically use the configured schema or + // "${projectDir}/nextflow_schema.json" as default. This input should not be empty + // for meta pipelines + + main: + + // + // Print parameter summary to stdout. This will display the parameters + // that differ from the default given in the JSON schema + // + if(parameters_schema) { + log.info paramsSummaryLog(input_workflow, parameters_schema:parameters_schema) + } else { + log.info paramsSummaryLog(input_workflow) + } + + // + // Validate the parameters using nextflow_schema.json or the schema + // given via the validation.parametersSchema configuration option + // + if(validate_params) { + if(parameters_schema) { + validateParameters(parameters_schema:parameters_schema) + } else { + validateParameters() + } + } + + emit: + dummy_emit = true +} + diff --git a/subworkflows/nf-core/utils_nfschema_plugin/meta.yml b/subworkflows/nf-core/utils_nfschema_plugin/meta.yml new file mode 100644 index 0000000..f7d9f02 --- /dev/null +++ b/subworkflows/nf-core/utils_nfschema_plugin/meta.yml @@ -0,0 +1,35 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "utils_nfschema_plugin" +description: Run nf-schema to validate parameters and create a summary of changed parameters +keywords: + - validation + - JSON schema + - plugin + - parameters + - summary +components: [] +input: + - input_workflow: + type: object + description: | + The workflow object of the used pipeline. + This object contains meta data used to create the params summary log + - validate_params: + type: boolean + description: Validate the parameters and error if invalid. + - parameters_schema: + type: string + description: | + Path to the parameters JSON schema. + This has to be the same as the schema given to the `validation.parametersSchema` config + option. When this input is empty it will automatically use the configured schema or + "${projectDir}/nextflow_schema.json" as default. The schema should not be given in this way + for meta pipelines. +output: + - dummy_emit: + type: boolean + description: Dummy emit to make nf-core subworkflows lint happy +authors: + - "@nvnieuwk" +maintainers: + - "@nvnieuwk" diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test new file mode 100644 index 0000000..842dc43 --- /dev/null +++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test @@ -0,0 +1,117 @@ +nextflow_workflow { + + name "Test Subworkflow UTILS_NFSCHEMA_PLUGIN" + script "../main.nf" + workflow "UTILS_NFSCHEMA_PLUGIN" + + tag "subworkflows" + tag "subworkflows_nfcore" + tag "subworkflows/utils_nfschema_plugin" + tag "plugin/nf-schema" + + config "./nextflow.config" + + test("Should run nothing") { + + when { + + params { + test_data = '' + } + + workflow { + """ + validate_params = false + input[0] = workflow + input[1] = validate_params + input[2] = "" + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should validate params") { + + when { + + params { + test_data = '' + outdir = 1 + } + + workflow { + """ + validate_params = true + input[0] = workflow + input[1] = validate_params + input[2] = "" + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ Validation of pipeline parameters failed!') } } + ) + } + } + + test("Should run nothing - custom schema") { + + when { + + params { + test_data = '' + } + + workflow { + """ + validate_params = false + input[0] = workflow + input[1] = validate_params + input[2] = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json" + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should validate params - custom schema") { + + when { + + params { + test_data = '' + outdir = 1 + } + + workflow { + """ + validate_params = true + input[0] = workflow + input[1] = validate_params + input[2] = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json" + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ Validation of pipeline parameters failed!') } } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config new file mode 100644 index 0000000..0907ac5 --- /dev/null +++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config @@ -0,0 +1,8 @@ +plugins { + id "nf-schema@2.1.0" +} + +validation { + parametersSchema = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json" + monochromeLogs = true +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json similarity index 95% rename from subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json rename to subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json index 7626c1c..331e0d2 100644 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json +++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json @@ -1,10 +1,10 @@ { - "$schema": "http://json-schema.org/draft-07/schema", + "$schema": "https://json-schema.org/draft/2020-12/schema", "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", "title": ". pipeline parameters", "description": "", "type": "object", - "definitions": { + "$defs": { "input_output_options": { "title": "Input/output options", "type": "object", @@ -87,10 +87,10 @@ }, "allOf": [ { - "$ref": "#/definitions/input_output_options" + "$ref": "#/$defs/input_output_options" }, { - "$ref": "#/definitions/generic_options" + "$ref": "#/$defs/generic_options" } ] } diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf deleted file mode 100644 index 2585b65..0000000 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf +++ /dev/null @@ -1,62 +0,0 @@ -// -// Subworkflow that uses the nf-validation plugin to render help text and parameter summary -// - -/* -======================================================================================== - IMPORT NF-VALIDATION PLUGIN -======================================================================================== -*/ - -include { paramsHelp } from 'plugin/nf-validation' -include { paramsSummaryLog } from 'plugin/nf-validation' -include { validateParameters } from 'plugin/nf-validation' - -/* -======================================================================================== - SUBWORKFLOW DEFINITION -======================================================================================== -*/ - -workflow UTILS_NFVALIDATION_PLUGIN { - - take: - print_help // boolean: print help - workflow_command // string: default commmand used to run pipeline - pre_help_text // string: string to be printed before help text and summary log - post_help_text // string: string to be printed after help text and summary log - validate_params // boolean: validate parameters - schema_filename // path: JSON schema file, null to use default value - - main: - - log.debug "Using schema file: ${schema_filename}" - - // Default values for strings - pre_help_text = pre_help_text ?: '' - post_help_text = post_help_text ?: '' - workflow_command = workflow_command ?: '' - - // - // Print help message if needed - // - if (print_help) { - log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text - System.exit(0) - } - - // - // Print parameter summary to stdout - // - log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text - - // - // Validate parameters relative to the parameter JSON schema - // - if (validate_params){ - validateParameters(parameters_schema: schema_filename) - } - - emit: - dummy_emit = true -} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml deleted file mode 100644 index 3d4a6b0..0000000 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml +++ /dev/null @@ -1,44 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json -name: "UTILS_NFVALIDATION_PLUGIN" -description: Use nf-validation to initiate and validate a pipeline -keywords: - - utility - - pipeline - - initialise - - validation -components: [] -input: - - print_help: - type: boolean - description: | - Print help message and exit - - workflow_command: - type: string - description: | - The command to run the workflow e.g. "nextflow run main.nf" - - pre_help_text: - type: string - description: | - Text to print before the help message - - post_help_text: - type: string - description: | - Text to print after the help message - - validate_params: - type: boolean - description: | - Validate the parameters and error if invalid. - - schema_filename: - type: string - description: | - The filename of the schema to validate against. -output: - - dummy_emit: - type: boolean - description: | - Dummy emit to make nf-core subworkflows lint happy -authors: - - "@adamrtalbot" -maintainers: - - "@adamrtalbot" - - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test deleted file mode 100644 index 5784a33..0000000 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test +++ /dev/null @@ -1,200 +0,0 @@ -nextflow_workflow { - - name "Test Workflow UTILS_NFVALIDATION_PLUGIN" - script "../main.nf" - workflow "UTILS_NFVALIDATION_PLUGIN" - tag "subworkflows" - tag "subworkflows_nfcore" - tag "plugin/nf-validation" - tag "'plugin/nf-validation'" - tag "utils_nfvalidation_plugin" - tag "subworkflows/utils_nfvalidation_plugin" - - test("Should run nothing") { - - when { - - params { - monochrome_logs = true - test_data = '' - } - - workflow { - """ - help = false - workflow_command = null - pre_help_text = null - post_help_text = null - validate_params = false - schema_filename = "$moduleTestDir/nextflow_schema.json" - - input[0] = help - input[1] = workflow_command - input[2] = pre_help_text - input[3] = post_help_text - input[4] = validate_params - input[5] = schema_filename - """ - } - } - - then { - assertAll( - { assert workflow.success } - ) - } - } - - test("Should run help") { - - - when { - - params { - monochrome_logs = true - test_data = '' - } - workflow { - """ - help = true - workflow_command = null - pre_help_text = null - post_help_text = null - validate_params = false - schema_filename = "$moduleTestDir/nextflow_schema.json" - - input[0] = help - input[1] = workflow_command - input[2] = pre_help_text - input[3] = post_help_text - input[4] = validate_params - input[5] = schema_filename - """ - } - } - - then { - assertAll( - { assert workflow.success }, - { assert workflow.exitStatus == 0 }, - { assert workflow.stdout.any { it.contains('Input/output options') } }, - { assert workflow.stdout.any { it.contains('--outdir') } } - ) - } - } - - test("Should run help with command") { - - when { - - params { - monochrome_logs = true - test_data = '' - } - workflow { - """ - help = true - workflow_command = "nextflow run noorg/doesntexist" - pre_help_text = null - post_help_text = null - validate_params = false - schema_filename = "$moduleTestDir/nextflow_schema.json" - - input[0] = help - input[1] = workflow_command - input[2] = pre_help_text - input[3] = post_help_text - input[4] = validate_params - input[5] = schema_filename - """ - } - } - - then { - assertAll( - { assert workflow.success }, - { assert workflow.exitStatus == 0 }, - { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, - { assert workflow.stdout.any { it.contains('Input/output options') } }, - { assert workflow.stdout.any { it.contains('--outdir') } } - ) - } - } - - test("Should run help with extra text") { - - - when { - - params { - monochrome_logs = true - test_data = '' - } - workflow { - """ - help = true - workflow_command = "nextflow run noorg/doesntexist" - pre_help_text = "pre-help-text" - post_help_text = "post-help-text" - validate_params = false - schema_filename = "$moduleTestDir/nextflow_schema.json" - - input[0] = help - input[1] = workflow_command - input[2] = pre_help_text - input[3] = post_help_text - input[4] = validate_params - input[5] = schema_filename - """ - } - } - - then { - assertAll( - { assert workflow.success }, - { assert workflow.exitStatus == 0 }, - { assert workflow.stdout.any { it.contains('pre-help-text') } }, - { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, - { assert workflow.stdout.any { it.contains('Input/output options') } }, - { assert workflow.stdout.any { it.contains('--outdir') } }, - { assert workflow.stdout.any { it.contains('post-help-text') } } - ) - } - } - - test("Should validate params") { - - when { - - params { - monochrome_logs = true - test_data = '' - outdir = 1 - } - workflow { - """ - help = false - workflow_command = null - pre_help_text = null - post_help_text = null - validate_params = true - schema_filename = "$moduleTestDir/nextflow_schema.json" - - input[0] = help - input[1] = workflow_command - input[2] = pre_help_text - input[3] = post_help_text - input[4] = validate_params - input[5] = schema_filename - """ - } - } - - then { - assertAll( - { assert workflow.failed }, - { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } - ) - } - } -} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml deleted file mode 100644 index 60b1cff..0000000 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -subworkflows/utils_nfvalidation_plugin: - - subworkflows/nf-core/utils_nfvalidation_plugin/** diff --git a/workflows/rangeland.nf b/workflows/rangeland.nf index 568c5a7..80929de 100644 --- a/workflows/rangeland.nf +++ b/workflows/rangeland.nf @@ -3,10 +3,9 @@ IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ - include { FASTQC } from '../modules/nf-core/fastqc/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMap } from 'plugin/nf-schema' include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_rangeland_pipeline' @@ -21,12 +20,10 @@ workflow RANGELAND { take: ch_samplesheet // channel: samplesheet read in from --input - main: ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() - // // MODULE: Run FastQC // @@ -42,11 +39,12 @@ workflow RANGELAND { softwareVersionsToYAML(ch_versions) .collectFile( storeDir: "${params.outdir}/pipeline_info", - name: 'nf_core_pipeline_software_mqc_versions.yml', + name: 'nf_core_' + 'pipeline_software_' + 'mqc_' + 'versions.yml', sort: true, newLine: true ).set { ch_collated_versions } + // // MODULE: MultiQC // @@ -62,15 +60,14 @@ workflow RANGELAND { summary_params = paramsSummaryMap( workflow, parameters_schema: "nextflow_schema.json") ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) - + ch_multiqc_files = ch_multiqc_files.mix( + ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) ch_methods_description = Channel.value( methodsDescriptionText(ch_multiqc_custom_methods_description)) - ch_multiqc_files = ch_multiqc_files.mix( - ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) ch_multiqc_files = ch_multiqc_files.mix( ch_methods_description.collectFile( @@ -83,12 +80,14 @@ workflow RANGELAND { ch_multiqc_files.collect(), ch_multiqc_config.toList(), ch_multiqc_custom_config.toList(), - ch_multiqc_logo.toList() + ch_multiqc_logo.toList(), + [], + [] ) - emit: - multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html + emit:multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html versions = ch_versions // channel: [ path(versions.yml) ] + } /* From 7d32cb3e54ffa154b3bd634ebea86a0b39d3685e Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Fri, 18 Oct 2024 10:24:32 +0200 Subject: [PATCH 47/61] Merge branch 'dev' into nf-core-template-merge-3.0.2 --- .github/workflows/awsfulltest.yml | 1 - CITATIONS.md | 10 +- README.md | 82 ++-- bin/merge_boa.r | 44 ++ bin/merge_qai.r | 38 ++ bin/test.R | 193 +++++++++ conf/base.config | 1 - conf/modules.config | 113 ++++- conf/test.config | 46 +- conf/test_full.config | 47 ++- docs/images/fonda_logo2_cropped.png | Bin 0 -> 77437 bytes docs/output.md | 96 ++++- docs/usage.md | 316 ++++++++++++-- main.nf | 13 +- modules.json | 8 +- modules/local/check_results.nf | 41 ++ modules/local/check_results_full.nf | 36 ++ modules/local/force-generate_analysis_mask.nf | 31 ++ .../local/force-generate_tile_allow_list.nf | 32 ++ modules/local/force-higher_level.nf | 46 ++ modules/local/force-mosaic.nf | 38 ++ modules/local/force-preprocess.nf | 50 +++ modules/local/force-pyramid.nf | 33 ++ modules/local/higher_level_force_config.nf | 83 ++++ modules/local/merge.nf | 56 +++ modules/local/preprocess_force_config.nf | 60 +++ modules/nf-core/fastqc/environment.yml | 5 - modules/nf-core/fastqc/main.nf | 64 --- modules/nf-core/fastqc/meta.yml | 66 --- modules/nf-core/fastqc/tests/main.nf.test | 309 -------------- .../nf-core/fastqc/tests/main.nf.test.snap | 392 ------------------ modules/nf-core/fastqc/tests/tags.yml | 2 - modules/nf-core/untar/main.nf | 63 +++ modules/nf-core/untar/meta.yml | 46 ++ nextflow.config | 61 ++- nextflow_schema.json | 199 ++++++++- subworkflows/local/higher_level.nf | 51 +++ subworkflows/local/preprocessing.nf | 83 ++++ .../utils_nfcore_rangeland_pipeline/main.nf | 37 +- workflows/rangeland.nf | 140 ++++++- 40 files changed, 2016 insertions(+), 1016 deletions(-) create mode 100755 bin/merge_boa.r create mode 100755 bin/merge_qai.r create mode 100755 bin/test.R create mode 100644 docs/images/fonda_logo2_cropped.png create mode 100644 modules/local/check_results.nf create mode 100644 modules/local/check_results_full.nf create mode 100644 modules/local/force-generate_analysis_mask.nf create mode 100644 modules/local/force-generate_tile_allow_list.nf create mode 100644 modules/local/force-higher_level.nf create mode 100644 modules/local/force-mosaic.nf create mode 100644 modules/local/force-preprocess.nf create mode 100644 modules/local/force-pyramid.nf create mode 100644 modules/local/higher_level_force_config.nf create mode 100644 modules/local/merge.nf create mode 100644 modules/local/preprocess_force_config.nf delete mode 100644 modules/nf-core/fastqc/environment.yml delete mode 100644 modules/nf-core/fastqc/main.nf delete mode 100644 modules/nf-core/fastqc/meta.yml delete mode 100644 modules/nf-core/fastqc/tests/main.nf.test delete mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap delete mode 100644 modules/nf-core/fastqc/tests/tags.yml create mode 100644 modules/nf-core/untar/main.nf create mode 100644 modules/nf-core/untar/meta.yml create mode 100644 subworkflows/local/higher_level.nf create mode 100644 subworkflows/local/preprocessing.nf diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 1273346..fb7ee45 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -32,7 +32,6 @@ jobs: test $CURRENT_APPROVALS_COUNT -ge 2 || exit 1 # At least 2 approvals are required - name: Launch workflow via Seqera Platform uses: seqeralabs/action-tower-launch@v2 - # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters with: diff --git a/CITATIONS.md b/CITATIONS.md index 9e3a2b5..db0724d 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -1,5 +1,11 @@ # nf-core/rangeland: Citations +## [FORCE on Nextflow](https://www.informatik.hu-berlin.de/de/forschung/gebiete/wbi/research/publications/2021/force_nextflow.pdf/@@download/file/force_nextflow.pdf) + +This paper describes technical details of deploying the FORCE tool in a Nextflow workflow. The workflow developed in this paper is the foundation for this nf-core pipeline. + +> Lehmann, F., Frantz, D., Becker, S., Leser, U., Hostert, P. (2021). FORCE on Nextflow: Scalable Analysis of Earth Observation Data on Commodity Clusters. In CIKM Workshops. + ## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) > Ewels PA, Peltzer A, Fillinger S, Patel H, Alneberg J, Wilm A, Garcia MU, Di Tommaso P, Nahnsen S. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol. 2020 Mar;38(3):276-278. doi: 10.1038/s41587-020-0439-x. PubMed PMID: 32055031. @@ -10,9 +16,9 @@ ## Pipeline tools -- [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) +- [FORCE](https://www.mdpi.com/2072-4292/11/9/1124) -> Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. + > Frantz, D. (2019). FORCE—Landsat + Sentinel-2 Analysis Ready Data and Beyond. Remote Sensing, 11, 1124 - [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) diff --git a/README.md b/README.md index f76d338..ecdf898 100644 --- a/README.md +++ b/README.md @@ -19,50 +19,36 @@ ## Introduction -**nf-core/rangeland** is a bioinformatics pipeline that ... +**nf-core/rangeland** is a geographical best-practice analysis pipeline for remotely sensed imagery. The pipeline processes satellite imagery alongside auxiliary data in multiple steps to arrive at a set of trend files related to land-cover changes. The main pipeline steps are: - +1. Read satellite imagery, digital elevation model, endmember definition, water vapor database and area of interest definition +2. Generate allow list and analysis mask to determine which pixels from the satellite data can be used +3. Preprocess data to obtain atmospherically corrected images alongside quality assurance information +4. Classify pixels by applying linear spectral unmixing +5. Time series analyses to obtain trends in vegetation dynamics +6. Create mosaic and pyramid visualizations of the results - - - -1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) -2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) +7. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) +8. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) ## Usage > [!NOTE] > If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. - +To run the pipeline on real data, input data needs to be acquired. Concretely, satellite imagery, water vapor data, a digital elevation model, endmember definitions, a datacube specification, and a area-of-interest specification are required. Please refer to the [usage documentation](https://nf-co.re/rangeland/usage) for details on the input structure. Now, you can run the pipeline using: - - ```bash -nextflow run nf-core/rangeland \ +nextflow run nf-core/rangeland/main.nf \ -profile \ - --input samplesheet.csv \ + --input \ + --dem \ + --wvdb \ + --data_cube \ + --aoi \ + --endmember \ --outdir ``` @@ -79,11 +65,33 @@ For more details about the output files and reports, please refer to the ## Credits -nf-core/rangeland was originally written by Fabian Lehmann, David Frantz, Felix Kummer. +The rangeland workflow was originally written by: + +- [Fabian Lehmann](https://github.com/Lehmann-Fabian) +- [David Frantz](https://github.com/davidfrantz) + +The original workflow can be found on [github](https://github.com/CRC-FONDA/FORCE2NXF-Rangeland). + +Transformation to nf-core/rangeland was conducted by [Felix Kummer](https://github.com/Felix-Kummer). nf-core alignment started on the [nf-core branch of the original repository](https://github.com/CRC-FONDA/FORCE2NXF-Rangeland/tree/nf-core). We thank the following people for their extensive assistance in the development of this pipeline: - +- [Fabian Lehmann](https://github.com/Lehmann-Fabian) +- [Katarzyna Ewa Lewinska](https://github.com/kelewinska). + +## Acknowledgements + +This pipeline was developed and aligned with nf-core as part of the [Foundations of Workflows for Large-Scale Scientific Data Analysis (FONDA)](https://fonda.hu-berlin.de/) initiative. + +[![FONDA](docs/images/fonda_logo2_cropped.png)](https://fonda.hu-berlin.de/) + +FONDA can be cited as follows: + +> **The Collaborative Research Center FONDA.** +> +> Ulf Leser, Marcus Hilbrich, Claudia Draxl, Peter Eisert, Lars Grunske, Patrick Hostert, Dagmar Kainmüller, Odej Kao, Birte Kehr, Timo Kehrer, Christoph Koch, Volker Markl, Henning Meyerhenke, Tilmann Rabl, Alexander Reinefeld, Knut Reinert, Kerstin Ritter, Björn Scheuermann, Florian Schintke, Nicole Schweikardt, Matthias Weidlich. +> +> _Datenbank Spektrum_ 2021 doi: [10.1007/s13222-021-00397-5](https://doi.org/10.1007/s13222-021-00397-5) ## Contributions and Support @@ -96,8 +104,6 @@ For further information or help, don't hesitate to get in touch on the [Slack `# - - An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. You can cite the `nf-core` publication as follows: @@ -107,3 +113,9 @@ You can cite the `nf-core` publication as follows: > Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen. > > _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x). + +This pipeline is based one the publication listed below. The publication can be cited as follows: + +> **FORCE on Nextflow: Scalable Analysis of Earth Observation Data on Commodity Clusters** +> +> [Lehmann, F., Frantz, D., Becker, S., Leser, U., Hostert, P. (2021). FORCE on Nextflow: Scalable Analysis of Earth Observation Data on Commodity Clusters. In CIKM Workshops.](https://www.informatik.hu-berlin.de/de/forschung/gebiete/wbi/research/publications/2021/force_nextflow.pdf/@@download/file/force_nextflow.pdf) diff --git a/bin/merge_boa.r b/bin/merge_boa.r new file mode 100755 index 0000000..27779ad --- /dev/null +++ b/bin/merge_boa.r @@ -0,0 +1,44 @@ +#!/usr/bin/env Rscript + +args = commandArgs(trailingOnly=TRUE) + + +if (length(args) < 3) { + stop("\nthis program needs at least 3 inputs\n1: output filename\n2-*: input files", call.=FALSE) +} + +fout <- args[1] +finp <- args[2:length(args)] +nf <- length(finp) + +require(raster) + + +img <- brick(finp[1]) +nc <- ncell(img) +nb <- nbands(img) + + +sum <- matrix(0, nc, nb) +num <- matrix(0, nc, nb) + +for (i in 1:nf){ + + data <- brick(finp[i])[] + + num <- num + !is.na(data) + + data[is.na(data)] <- 0 + sum <- sum + data + +} + +mean <- sum/num +img[] <- mean + + +writeRaster(img, filename = fout, format = "GTiff", datatype = "INT2S", + options = c("INTERLEAVE=BAND", "COMPRESS=LZW", "PREDICTOR=2", + "NUM_THREADS=ALL_CPUS", "BIGTIFF=YES", + sprintf("BLOCKXSIZE=%s", img@file@blockcols[1]), + sprintf("BLOCKYSIZE=%s", img@file@blockrows[1]))) diff --git a/bin/merge_qai.r b/bin/merge_qai.r new file mode 100755 index 0000000..fc9066f --- /dev/null +++ b/bin/merge_qai.r @@ -0,0 +1,38 @@ +#!/usr/bin/env Rscript + +args = commandArgs(trailingOnly=TRUE) + + +if (length(args) < 3) { + stop("\nthis program needs at least 3 inputs\n1: output filename\n2-*: input files", call.=FALSE) +} + +fout <- args[1] +finp <- args[2:length(args)] +nf <- length(finp) + +require(raster) + + +img <- raster(finp[1]) +nc <- ncell(img) + + +last <- rep(1, nc) + +for (i in 1:nf){ + + data <- raster(finp[i])[] + + last[!is.na(data)] <- data[!is.na(data)] + +} + +img[] <- last + + +writeRaster(img, filename = fout, format = "GTiff", datatype = "INT2S", + options = c("INTERLEAVE=BAND", "COMPRESS=LZW", "PREDICTOR=2", + "NUM_THREADS=ALL_CPUS", "BIGTIFF=YES", + sprintf("BLOCKXSIZE=%s", img@file@blockcols[1]), + sprintf("BLOCKYSIZE=%s", img@file@blockrows[1]))) diff --git a/bin/test.R b/bin/test.R new file mode 100755 index 0000000..2c5b0d5 --- /dev/null +++ b/bin/test.R @@ -0,0 +1,193 @@ +#!/usr/bin/env Rscript + +args = commandArgs(trailingOnly=TRUE) + + +if (length(args) != 7 && length(args) != 2) { + stop("\n Error: wrong number of parameters. Usage: \n 1st arg: workflow results directory (mosaic) + \n 2nd-7th args: reference rasters (*.tif) in order: + woody cover change, woody cover year of change, + herbaceous cover change, herbaceous cover year of change, + peak change, peak year of change + \nor \n + 1st arg: workflow results directory (mosaic) \n 2nd arg: reference directory + ", call.=FALSE) +} + +# load package +require(terra) + +# function to compare change directions +compare_direction <- function(r1, r2, threshold = 0.95) { + + # get signs + s1 <- sign(r1) + s2 <- sign(r2) + + # replace na's + vals1 <- subst(s1, NA, -9999) + vals2 <- subst(s2, NA, -9999) + + # Compare the signs + matches <- vals1 == vals2 + match_count <- sum(values(matches)) + total_count <- sum(!is.na(values(vals1))) + + # Calculate the percentage of matches + match_percentage <- match_count / total_count + + if (match_percentage >= threshold) { + return(TRUE) + } else { + return(paste("Change directions not matching. Match percentage:", match_percentage)) + } +} + + +# LOAD REFERENCE +####################################################################### + +if (length(args) == 7 ){ + woody_cover_changes_ref <- rast(args[2]) + woody_cover_year_of_change_ref <- rast(args[3]) + + herbaceous_cover_changes_ref <- rast(args[4]) + herbaceous_cover_year_of_change_ref <- rast(args[5]) + + peak_changes_ref <- rast(args[6]) + peak_year_of_change_ref <- rast(args[7]) +} else { + # reference parent dir + ref_dir <- args[2] + + vrt_file <- list.files(ref_dir, pattern = "VBL-CAO\\.vrt$", recursive = TRUE, full.names = TRUE) + woody_ref <- rast(vrt_file) + woody_cover_changes_ref <- woody_ref$CHANGE + woody_cover_year_of_change_ref <- woody_ref["YEAR-OF-CHANGE"] + + vrt_file <- list.files(ref_dir, pattern = "VSA-CAO\\.vrt$", recursive = TRUE, full.names = TRUE) + herbaceous_ref <- rast(vrt_file) + herbaceous_cover_changes_ref <- herbaceous_ref$CHANGE + herbaceous_cover_year_of_change_ref <- herbaceous_ref["YEAR-OF-CHANGE"] + + vrt_file <- list.files(ref_dir, pattern = "VPS-CAO\\.vrt$", recursive = TRUE, full.names = TRUE) + peak_ref <- rast(vrt_file) + peak_changes_ref <- peak_ref$CHANGE + peak_year_of_change_ref <- peak_ref["YEAR-OF-CHANGE"] +} + +# WOODY COVER CHANGE (VALUE OF BASE LEVEL) +####################################################################### + +# input data dir +dinp <- args[1] + +fname <- dir(dinp, ".*HL_TSA_LNDLG_SMA_VBL-CAO.vrt$", full.names=TRUE) + +woody_cover_rast <- rast(fname) + +woody_cover_changes <- woody_cover_rast$CHANGE +woody_cover_year_of_change <- woody_cover_rast["YEAR-OF-CHANGE"] + + + +# HERBACEOUS COVER CHANGE (VALUE OF SEASONAL APLITUDE) +####################################################################### + + +fname <- dir(dinp, ".*HL_TSA_LNDLG_SMA_VSA-CAO.vrt$", full.names=TRUE) + +herbaceous_cover_rast <- rast(fname) + +herbaceous_cover_changes <- herbaceous_cover_rast$CHANGE +herbaceous_cover_year_of_change <- herbaceous_cover_rast["YEAR-OF-CHANGE"] + + + +# VALUE OF PEAK SEASON +####################################################################### + +fname <- dir(dinp, ".*HL_TSA_LNDLG_SMA_VPS-CAO.vrt$", full.names=TRUE) + +peak_rast <- rast(fname) + +peak_changes <- peak_rast$CHANGE +peak_year_of_change <- peak_rast["YEAR-OF-CHANGE"] + + + +# FOR REFERENCE: SAVE RASTERS +####################################################################### + +#writeRaster(woody_cover_changes, "woody_cover_chg_ref.tif") +#writeRaster(woody_cover_year_of_change, "woody_cover_yoc_ref.tif") + +#writeRaster(herbaceous_cover_changes, "herbaceous_cover_chg_ref.tif") +#writeRaster(herbaceous_cover_year_of_change, "herbaceous_cover_yoc_ref.tif") + +#writeRaster(peak_changes, "peak_chg_ref.tif") +#writeRaster(peak_year_of_change, "peak_yoc_ref.tif") + + + + +# COMPARE TESTRUN WITH REFERENCE EXECUTION +####################################################################### +failure <- FALSE + +woody_cover_changes_result <- compare_direction(woody_cover_changes, woody_cover_changes_ref) +if (is.character(woody_cover_changes_result)) { + print(paste0("Error: ", woody_cover_changes_result, " for woody cover changes.")) + failure <- TRUE +} else { + print("Woody cover change check passed.") +} + +woody_cover_year_of_change_result <- all.equal(woody_cover_year_of_change, woody_cover_year_of_change_ref, tolerance=1e-3) +if (is.character(woody_cover_year_of_change_result)) { + print(paste0("Error: ", woody_cover_year_of_change_result, " for woody cover year of change.")) + failure <- TRUE +} else { + print("Woody cover year of change check passed.") +} + + +herbaceous_cover_changes_result <- compare_direction(herbaceous_cover_changes, herbaceous_cover_changes_ref) +if (is.character(herbaceous_cover_changes_result)) { + print(paste0("Error: ",herbaceous_cover_changes_result, " for herbaceous cover changes.")) + failure <- TRUE +} else { + print("Herbaceous cover change check passed.") +} + +herbaceous_cover_year_of_change_result <- all.equal(herbaceous_cover_year_of_change, herbaceous_cover_year_of_change_ref, tolerance=1e-3) +if (is.character(herbaceous_cover_year_of_change_result)) { + print(paste0("Error: ", herbaceous_cover_year_of_change_result, " for herbaceous cover year of change.")) + failure <- TRUE +} else { + print("Herbaceous cover year of change check passed.") +} + + +peak_changes_result <- compare_direction(peak_changes, peak_changes_ref) +if (is.character(peak_changes_result)) { + print(paste0("Error: ", peak_changes_result, " for peak changes.")) + failure <- TRUE +} else { + print("Peak change check passed.") +} + + +peak_year_of_change_result <- all.equal(peak_year_of_change, peak_year_of_change_ref, tolerance=1e-3) +if (is.character(peak_year_of_change_result)) { + print(paste0("Error: ", peak_year_of_change_result, " for peak year of change.")) + failure <- TRUE +} else { + print("Peak year of change check passed.") +} + +if (failure) { + stop("Some test failed.") +} else { + print("All checks passed.") +} diff --git a/conf/base.config b/conf/base.config index 60219f7..7dde9f4 100644 --- a/conf/base.config +++ b/conf/base.config @@ -24,7 +24,6 @@ process { // These labels are used and recognised by default in DSL2 files hosted on nf-core/modules. // If possible, it would be nice to keep the same label naming convention when // adding in your local modules too. - // TODO nf-core: Customise requirements for specific processes. // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors withLabel:process_single { cpus = { 1 } diff --git a/conf/modules.config b/conf/modules.config index d266a38..27ca7c4 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -13,19 +13,118 @@ process { publishDir = [ - path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }, - mode: params.publish_dir_mode, + path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }, + mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: FASTQC { - ext.args = '--quiet' + withName: "FORCE_GENERATE_ANALYSIS_MASK" { + publishDir = [ + path: { "${params.outdir}/preparation/" }, + mode: params.publish_dir_mode, + pattern: '**.tif' + ] + } + + withName: "FORCE_GENERATE_TILE_ALLOW_LIST" { + publishDir = [ + path: { "${params.outdir}/preparation/" }, + pattern: '*.txt', + mode: params.publish_dir_mode + ] + } + + withName: "FORCE_PREPROCESS" { + errorStrategy = 'retry' + maxRetries = 5 + publishDir = [ + [ + path: { "${params.outdir}/preprocess/${task.tag}/logs" }, + mode: params.publish_dir_mode, + pattern: '*.log' + ], + [ + path: { "${params.outdir}/preprocess/${task.tag}" }, + mode: 'symlink', + pattern: 'level2_ard/**/*' + ] + ] + } + + + withName: "HIGHER_LEVEL_CONFIG" { + errorStrategy = 'retry' + maxRetries = 5 + publishDir = [ + path: { "${params.outdir}/higher-level/${task.tag}/param_files" }, + mode: params.publish_dir_mode, + pattern: '*.prm' + ] + } + + withName: "FORCE_HIGHER_LEVEL" { + publishDir = [ + path: { "${params.outdir}/higher-level/${task.tag}" }, + mode: 'symlink', + pattern: 'trend/*.tif', + saveAs: { "trend_files/${it.tokenize('/')[-1]}" } + ] + } + + withName: "FORCE_PYRAMID" { + stageInMode = 'copy' + publishDir = [ + [ + path: { "${params.outdir}/trend/pyramid/" }, + saveAs: { "${it.substring(12,it.indexOf("."))}/trend/${it.substring(0,11)}/$it" }, + pattern: '*.tif*', + mode: params.publish_dir_mode + ], + [ + path: { "${params.outdir}/trend/pyramid/" }, + pattern: 'versions.yml', + mode: params.publish_dir_mode + ] + ] + } + + withName: "FORCE_MOSAIC" { + publishDir = [ + path: { "${params.outdir}/trend/mosaic/" }, + mode: params.publish_dir_mode, + saveAs: {"${params.outdir}/trend/mosaic/${task.tag}/${it.replaceAll("trend/","")}"} + ] + } + + withName: "CHECK_RESULTS" { + errorStrategy = { task.exitStatus == 143 ? 'retry' : 'ignore' } + publishDir = [ + enabled: false + ] + } + + withName: "PREPROCESS_CONFIG" { + errorStrategy = 'retry' + maxRetries = 5 + publishDir = [ + path: { "${params.outdir}/preprocess/${task.tag}/param_files" }, + mode: params.publish_dir_mode, + pattern: '*.prm' + ] } withName: 'MULTIQC' { - ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } + ext.args = params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' + publishDir = [ + path: { "${params.outdir}/multiqc" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: "UNTAR_*" { publishDir = [ - path: { "${params.outdir}/multiqc" }, - mode: params.publish_dir_mode, + path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }, + mode: 'symlink', saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } diff --git a/conf/test.config b/conf/test.config index 879ccb5..b84814c 100644 --- a/conf/test.config +++ b/conf/test.config @@ -23,9 +23,47 @@ params { config_profile_description = 'Minimal test dataset to check pipeline function' // Input data - // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets - // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' + input = 'https://github.com/nf-core/test-datasets/raw/rangeland/Landsat_collection2/Landsat_data.tar.gz' + dem = 'https://github.com/nf-core/test-datasets/raw/rangeland/dem/dem.tar.gz' + wvdb = 'https://github.com/nf-core/test-datasets/raw/rangeland/wvp/wvdb.tar.gz' - + input_tar = true + dem_tar = true + wvdb_tar = true + + data_cube = 'https://github.com/nf-core/test-datasets/raw/rangeland/datacube/datacube-definition.prj' + aoi = 'https://github.com/nf-core/test-datasets/raw/rangeland/vector/aoi.gpkg' + endmember = 'https://github.com/nf-core/test-datasets/raw/rangeland/endmember/hostert-2003.txt' + + // Remote sensing imagery parameters + start_date = '1987-01-01' + end_date = '1989-12-31' + + sensors_level1 = 'LT04,LT05' + sensors_level2 = 'LND04 LND05' + + // Reference data + woody_change_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/woody_cover_chg_ref.tif' + woody_yoc_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/woody_cover_yoc_ref.tif' + + herbaceous_change_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/herbaceous_cover_chg_ref.tif' + herbaceous_yoc_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/herbaceous_cover_yoc_ref.tif' + + peak_change_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/peak_chg_ref.tif' + peak_yoc_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/peak_yoc_ref.tif' + + // Other parameters + group_size = 10 + + // enable mosaic for result checking + mosaic_visualization = true + + validationSchemaIgnoreParams = "peak_yoc_ref,peak_change_ref,herbaceous_yoc_ref,herbaceous_change_ref,woody_yoc_ref,woody_change_ref,config_profile_description,config_profile_name" +} + +process { + withName: "UNTAR_*" { + container = 'docker.io/ubuntu:23.10' + ext.args2 = "--strip-components=0" + } } diff --git a/conf/test_full.config b/conf/test_full.config index 024a55c..907fdf8 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -14,11 +14,46 @@ params { config_profile_name = 'Full test profile' config_profile_description = 'Full test dataset to check pipeline function' - // Input data for full size test - // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) - // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' + // Input data + input = 's3://ngi-igenomes/test-data/rangeland/landsat.tar' + dem = 's3://ngi-igenomes/test-data/rangeland/dem.tar' + wvdb = 's3://ngi-igenomes/test-data/rangeland/wvdb.tar' - // Fasta references - fasta = params.pipelines_testdata_base_path + 'viralrecon/genome/NC_045512.2/GCF_009858895.2_ASM985889v3_genomic.200409.fna.gz' + input_tar = true + dem_tar = true + wvdb_tar = true + + data_cube = 's3://ngi-igenomes/test-data/rangeland/datacube-definition.prj' + aoi = 's3://ngi-igenomes/test-data/rangeland/aoi.gpkg' + endmember = 's3://ngi-igenomes/test-data/rangeland/hostert-2003.txt' + + + // Remote sensing imagery parameters + start_date = '1986-01-01' + end_date = '1989-12-31' + + sensors_level1 = 'LT04,LT05' + sensors_level2 = 'LND04 LND05' + + // enable time series stack output + return_tss = true + + // enable mosaic for result checking (required for result checking) + mosaic_visualization = true + + // reference data + reference = "s3://ngi-igenomes/test-data/rangeland/reference.tar" + + validationSchemaIgnoreParams = "reference" + +} + +process { + withName: "UNTAR_*" { + container = 'docker.io/ubuntu:23.10' + ext.args2 = "--strip-components=1" + } + withName: "UNTAR_REF"{ + ext.args2 = "--strip-components=0" + } } diff --git a/docs/images/fonda_logo2_cropped.png b/docs/images/fonda_logo2_cropped.png new file mode 100644 index 0000000000000000000000000000000000000000..1f49ddd8abfa5660da672fde84a972817f57fb33 GIT binary patch literal 77437 zcmeGEXHZk$_XUijs3<7d00997kq!z-FCwUPl-_#_U0P@%SO66ekrH}Q0tTd)Py>R} zdv75ky@UXvhrn}#`u)%R-~C=ZFP@oCW}La=y(j0av-jF-uXFL;GmWR0C>bfq$jB}! zD=BJ`kx^)qk)5tMe-8KwQ+jza85vcazm9>ImL>R>yN8<{$l3Omm!G@sEn8oZ9T}PL zcz%MDj}7wDn`2K}!_(%sTIW8V@zwD8IJcmn$`atbz+C=-zkF?u<9SH#tC(YKp&&7& zP|`L_K(d9A9Y3UR?u~EiG&fJhHG#XTQVOGz7R*uY3%I!z*d6|r-4;3GbVOyUId6KI zx!m!b>COmYeIqpT(~|)`&+_)5->)+j_Vz#IG(Jwp80Af=gIdslv|Z^o_=9_Kt8gf$G#L~x?Ry39Z;*~r#s;|Y?p`C3U6-N zZZT_Ls9M2S8rxMkhwwUC-p}1x;U(_Cj_0<+LZ)h-7hE-2j@@yz&>_EHamC?hy~)}0 za}WiNsw~`980)in%w_6Xd39Oaw43e=`0^jon<0+98D}friA@jT>1Q8f15SzlC=Ka~ zJ$KqK_O5O0w{l1B!56Q3>=w$m{Z*>rZ*{C;RKyLPw)zxKO*X!%@AUvRAET{9ncX!pinRmHj zdOM@)h=~)nOF@j+ZEeb=co&hA8Z;a6#Xik@Cqx!$UNy6v3P!sO8I`zNH4SF-Png~0 z@dIy6FAuYMJGen%xqnPTtzAqreWVhucb|%8MjBo$ zeM_^(#cgIfw5U9C8WGo**(y|7-ECuP4o*bsf8G1FmP2Gd!1XscJ*-xx|IqiU zuV=4#P1mtNF#Y^7U@q~LnLI

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Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline. +This document describes the output produced by the pipeline. The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. - - ## Pipeline overview The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -- [FastQC](#fastqc) - Raw read QC +- [untar](#untar) - Optionally extract input files +- [Preparation](#preparation)- Create a masks and boundaries for further analyses. +- [Preprocessing](#preprocessing) - Preprocessing of satellite imagery. +- [Higher-level-Processing](#higher-level-processing) - Classify preprocessed imagery and perform time series analyses. +- [Visualization](#visualization). Create two visualizations of the results. - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution -### FastQC +### Untar + +

    +Output files + +- `untar/` + - ``: directory containing symlinks to decompressed digital elevation input data. Only present if a tar archive was provided for the digital elevation model. Name of the directory derived from archive contents. + - ``: directory containing symlinks to decompressed water vapor input data. Only present if a tar archive was provided for water vapor data. Name of the directory derived from archive contents. + - ``: directory containing symlinks to decompressed satellite imagery input data. Only present if a tar archive was provided for satellite data. Name of the directory derived from archive contents. + +
    + +[untar](https://nf-co.re/modules/untar) is a nf-core module used to extract files from tar archives. + +Invokation of untar depends on certain parameters (i.e input_tar, dem_tar and wvdb_tar). Thus, the outputs files are only generated when these are set to true. + +### Preparation + +
    +Output files + +- `preparation/` + - `tile_allow.txt`: File containing all [FORCE](https://force-eo.readthedocs.io/en/latest/index.html) notation tiles of the earths surface that should be used further in the pipeline. The first line contains the number of tiles. Following lines contain tile identifiers. + - `mask/`: Directory containing a subdirectory for every [FORCE](https://force-eo.readthedocs.io/en/latest/index.html) tile. Each subdirectory contains the `aoi.tif` file. This file represents a binary mask layer that indicates which pixels are eligible for analyses. + +
    + +In the preparation step, usable tiles and pixels per tile are identified. + +[force-tile-extent](https://force-eo.readthedocs.io/en/latest/components/auxilliary/tile-extent.html#force-tile-extent) analyses the area of interest information and determines the tiles that can be used. These tiles are later used by other [FORCE](https://force-eo.readthedocs.io/en/latest/index.html) submodules. + +[force-cube](https://force-eo.readthedocs.io/en/latest/components/auxilliary/cube.html#force-cube) computes the usable pixels for each [FORCE](https://force-eo.readthedocs.io/en/latest/index.html) tile. This computation is based on the specified are of interest and the resolution. The resulting binary masks can be used to understand which pixels were discarded (e.g. because they only contain water). + +### Preprocessing + +
    +Output files + +- `preprocess//` + - `param_files/`: Directory containing parameter files for [FORCE](https://force-eo.readthedocs.io/en/latest/index.html) preprocessing modules. One file per satellite mission per tile. + - `level2_ard/`: Directory containing symlinks to analysis-ready-data. Subdirectories contain the .tif files that were generated during preprocessing. + - `logs/`: Logs from preprocessing. + +
    + +Preprocessing consist of two parts, generating parameter files and actual preprocessing. + +The parameter files created through [force-parameter](https://force-eo.readthedocs.io/en/latest/components/auxilliary/parameter.html#force-parameter) can be viewed to understand concrete preprocessing techniques applied for a given tile. + +Logs and analysis-ready-data (ARD) are generated using the [force-l2ps](https://force-eo.readthedocs.io/en/latest/components/lower-level/level2/l2ps.html) command. Logs can be consulted for debugging purposes. ARD may be collected as a basis for other remote sensing workflows. Moreover, ARD contains two .tif files per initial input image, a quality data file and the atmospherically corrected satellite data, that can be viewed using geographic information systems (GISs). Note that ARD data is only published as symbolic links due to the amount and size of the files. + +### Higher-level-Processing + +
    +Output files + +- `higher-level//` + - `param_files/`: Parameter files used in [force-higher-level](https://force-eo.readthedocs.io/en/latest/components/higher-level/index.html). + - `trend_files`: Symlinks to trend files that are the result of higher-level processing. + +
    + +Higher level processing consist of two parts, generating parameter files and performing various processing task as defined in the parameter files. + +Parameter files may be consulted to derive information about the specific processing task performed for a given tile. In this workflow, classification using spectral unmixing is performed. + +Spectral unmixing is a common technique to derive sub-pixel classification. Concretely, a set of endmember (provided using `--endmember`) is consulted to determine fractions of different types of vegetation, soil, $\ldots$ for each pixel. + +Next, time series analysis for different vegetation characteristics is performed. + +The resulting trend files can be consulted to view trends for individual tiles. They are saved as symlinks because of their large size. + +If the `return_tss` parameter was set to `true`, the pipeline will also output files with the `TSS` ending. These files contain the time series stack(TSS) for the given tile and index or band. Here, for each date of acquisition, an image is available that contains the values for that date. + +### Visualization
    Output files -- `fastqc/` - - `*_fastqc.html`: FastQC report containing quality metrics. - - `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. +- `trend/` + - `mosaic//` + - ``: Auxiliary files for the mosaic visualization. + - `mosaic`: Contains a single virtual raster file that defines the mosaic visualization. + - `pyramid//trend//`: Contains tile-wise pyramid visualizations for every trend analyzed in the workflow.
    -[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). +Two types of common visualizations are generated in the last step of the pipeline. They are results of [force-mosaic](https://force-eo.readthedocs.io/en/latest/components/auxilliary/mosaic.html) and [force-pyramid](https://force-eo.readthedocs.io/en/latest/components/auxilliary/pyramid.html). Note that these visualizations do not add more logic to the workflow but rather rearrange the output files of higher-level-processing. Both visualizations are enabled by default but may be disabled in a certain configuration files. Thus, these outputs are optional. ### MultiQC diff --git a/docs/usage.md b/docs/usage.md index da18515..390aa94 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -4,60 +4,306 @@ > _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ -## Introduction +## Input - +As most remote sensing workflows, this pipeline relies on numerous sources of data. In the following we will describe the required data and corresponding formats. Mandatory input data consists of satellite data, a digital elevation model, a water vapor database, a data_cube, an area-of-interest specification and an endmember definition. -## Samplesheet input +### Satellite data -You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. +This pipeline operates on Landsat data. Landsat is a joint NASA/U.S. Geolical Survey satellite mission that provides continuous Earth obersvation data since 1984 at 30m spatial resolution with a temporal revisit frequency of 8-16 days. +Landsast carries multispectral optical instruments that observe the land surface in the visible to shortwave infrared spectrum. +For infos on Landsat, see [here](https://www.usgs.gov/core-science-systems/nli/landsat). + +Satellite data should be given as a path to a common root of all imagery. This is a common format used in geographic information systems, including FORCE, which is applied in this pipeline. The expected structure underneath the root directory should follow this example: + +``` +root +├── 181035 +│ └── LE07_L1TP_181035_20061217_20170106_01_T1 +│ | ├── LE07_L1TP_181035_20061217_20170106_01_T1_ANG.txt +│ | ├── LE07_L1TP_181035_20061217_20170106_01_T1_B1.TIF +│ | ├── LE07_L1TP_181035_20061217_20170106_01_T1_B2.TIF +│ | ├── LE07_L1TP_181035_20061217_20170106_01_T1_B3.TIF +│ | ├── LE07_L1TP_181035_20061217_20170106_01_T1_B4.TIF +│ | ├── LE07_L1TP_181035_20061217_20170106_01_T1_B5.TIF +│ | ├── LE07_L1TP_181035_20061217_20170106_01_T1_B6_VCID_1.TIF +│ | ├── LE07_L1TP_181035_20061217_20170106_01_T1_B6_VCID_2.TIF +│ | ├── LE07_L1TP_181035_20061217_20170106_01_T1_B7.TIF +│ | ├── LE07_L1TP_181035_20061217_20170106_01_T1_B8.TIF +│ | ├── LE07_L1TP_181035_20061217_20170106_01_T1_BQA.TIF +│ | ├── LE07_L1TP_181035_20061217_20170106_01_T1_GCP.txt +│ | └── LE07_L1TP_181035_20061217_20170106_01_T1_MTL.txt +| └── ... +├── 181036 +│ └── LE07_L1TP_181036_20061217_20170105_01_T1 +│ | ├── LE07_L1TP_181036_20061217_20170105_01_T1_ANG.txt +│ | ├── LE07_L1TP_181036_20061217_20170105_01_T1_B1.TIF +│ | ├── LE07_L1TP_181036_20061217_20170105_01_T1_B2.TIF +│ | ├── LE07_L1TP_181036_20061217_20170105_01_T1_B3.TIF +│ | ├── LE07_L1TP_181036_20061217_20170105_01_T1_B4.TIF +│ | ├── LE07_L1TP_181036_20061217_20170105_01_T1_B5.TIF +│ | ├── LE07_L1TP_181036_20061217_20170105_01_T1_B6_VCID_1.TIF +│ | ├── LE07_L1TP_181036_20061217_20170105_01_T1_B6_VCID_2.TIF +│ | ├── LE07_L1TP_181036_20061217_20170105_01_T1_B7.TIF +│ | ├── LE07_L1TP_181036_20061217_20170105_01_T1_B8.TIF +│ | ├── LE07_L1TP_181036_20061217_20170105_01_T1_BQA.TIF +│ | ├── LE07_L1TP_181036_20061217_20170105_01_T1_GCP.txt +│ | └── LE07_L1TP_181036_20061217_20170105_01_T1_MTL.txt +| └── ... +└── ... +``` + +Subdirectories of root contain _path_ and _row_ information as commonly used for Landsat imagery. As an example, the sub directory `181036/` contains imagery for path 18 and row 1036. + +The next level of subdirectories contains the data for a specific day and from a specific source. Lets look at the example `LE07_L1TP_181036_20061217_20170105_01_T1`: + +- "LE07" corresponds to Landsat 7 Enhanced +- "L1TP" corresponds to Level-1 Terrain Corrected imagery +- "181036" corresponds to the path and row of the imagery, this should match the subdirectory +- "20061217" identifies the 17th December 2006 as the date of acquisition +- "20170105" identifies the 5th January 2017 as the date of (re)processing +- "01" corresponds to version number of the remote sensing product +- "T1" corresponds to the Tier of the data collection, which indicates the Tier 1 landsat collection in this case + +On the lowest level of the structure, the actual data is stored. Looking at the contents of `LE07_L1TP_181036_20061217_20170105_01_T1`, we see that all files share the same prefix, followed by a specification of the specific files contents. These suffixes include: + +- "B" followed by a number _i_ identifying the band of the satellite (band 6 has two files as Landsat 7 has two thermal bands) +- "BQA" identifying the quality information band +- "GCP" identifies ground control point information +- "ANG" identifies angle of observation and other geometric information information +- "MTL" identifies meta data + +All files within the lowest level of structure belong to a single observation. Files containing imagery (prefix starts with "B") should be .tif files. Files containing auxiliary data are text files. + +This structure is automatically generated when [using force to download the data](https://force-eo.readthedocs.io/en/latest/components/lower-level/level1/level1-csd.html?). We strongly suggest users to download data using FORCE (e.g.). For example, executing the following code (e.g. with [FORCE in docker](https://force-eo.readthedocs.io/en/latest/setup/docker.html)) will download data for Landsat 4,5 and 7, in the time range from 1st January 1984 until 31st December 2006, including pictures with up to 70 percent of cloud coverage: + +```bash +mkdir -p meta +force-level1-csd -u -s "LT04,LT05,LE07" meta +mkdir -p data +force-level1-csd -s "LT04,LT05,LE07" -d "19840101,20061231" -c 0,70 meta/ data/ queue.txt vector/aoi.gpkg +``` + +Note that you need to pass an area-of-interest file, see the area of interest section [Area of interest](#aoi) for details. + +The satellite imagery can be given to the pipeline using: + +```bash +--input '[path to imagery root]' +``` + +The satellite imagery can also be provide as a tar archive. In this case it is mandatory to set `--input_tar` to true. Moreover, within the tar archive, the structure explained above has to be in place. In the example above `181036/` and `181035/` would need to be in the top level of the archive. + +### Digital Elevation Model (DEM) + +A DEM is necessary for topographic correction of Landsat data, and helps to distinguish between cloud, shadows and water surfaces. Common sources for digital elevation models are [Copernicus](https://www.copernicus.eu/en),[Shuttle Radar Topography Mission](https://www2.jpl.nasa.gov/srtm/) (SRTM), or [Advanced Spaceborne Thermal Emission and Reflection Radiometer](https://asterweb.jpl.nasa.gov/) (ASTER). + +The pipeline expects a path to the Digital Elevation Model root directory as a parameter. Concretely, the expected structure would look like this: + +``` +dem +├── .vrt +└── / + └── ... +``` + +Here, `.vrt` orchestrates the single digital elevation files in the `` directory. + +The DEM can be given to the pipeline using: ```bash ---input '[path to samplesheet file]' +--dem '[path to dem root]' ``` -### Multiple runs of the same sample +The digital elevation model can also be provide as a tar archive. In this case it is mandatory to set `--dem_tar` to true. Moreover, within the tar archive, the structure explained above has to be in place. In the example above `.vrt` and `/` would need to be in the top level of the archive. -The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: +### Water Vapor Database (WVDB) -```csv title="samplesheet.csv" -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz +For atmospheric correction of Landsat data, information on the atmospheric water vapor content is necessary. + +The expected format for the wvdb is a directory containing daily water vapor measurements for the area of interest. + +We recommend using a precompiled water vapor database, like [this one](https://zenodo.org/record/4468701). +This global water vapor database can be downloaded by executing this code: + +```bash +wget -O wvp-global.tar.gz https://zenodo.org/record/4468701/files/wvp-global.tar.gz?download=1 +tar -xzf wvp-global.tar.gz --directory wvdb/ +rm wvp-global.tar.gz +``` + +The WVDB can be given to the pipeline using: + +```bash +--wvdb '[path to wvdb dir]' +``` + +The water vapor database can also be provide as a tar archive. In this case it is mandatory to set `--wvdb_tar` to true. All files of the wvdb would need to be in the top level of the archive. + +### Datacube + +The datacube definition stores information about the projection and reference grid of the generated datacube. For details see the [FORCE main paper](https://www.mdpi.com/2072-4292/11/9/1124). + +The datacube definition is passed as a single file using: + +```bash +--data_cube '[path to datacube definition file]' +``` + +### Area of interest (AOI) + + + +The area of interest is a geospatial vector dataset that holds the boundary of the targeted area. + +AOI is passed as a single using: + +```bash +--aoi '[path to area of interest file]' ``` -### Full samplesheet +### Endmember + +For unmixing satellite-observed reflectance into sub-pixel fractions of land surface components (e.g. photosynthetic active vegetation), endmember spectra are necessary. -The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below. +An example endmember definition (developed in [Hostert et al. 2003](https://www.sciencedirect.com/science/article/abs/pii/S0034425703001457)) looks like this: -A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. +``` +320 730 2620 0 +560 1450 3100 0 +450 2240 3340 0 +3670 2750 4700 0 +1700 4020 7240 0 +710 3220 5490 0 +``` -```csv title="samplesheet.csv" -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz -CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz -TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz, -TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, +Each colum represents a different endmember. Columns represent Landsat bands (R,G,B, NIR, SWIR1, SWIR2). + +The endmembers can be passed in a single text-file using: + +```bash +--endmember '[path to endmember]' ``` -| Column | Description | -| --------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | -| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +## Pipeline configuration + +Users can specify additional parameters to configure how the underlying workflow tools handle the provided data. + +### Sensor Levels + +Data from different satellites can be processed within this workflow. Users may wish to include different satellites in preprocessing and in higher level processing. To control this behavior, two parameters can be set when the pipeline is launched. +The first parameter - `sensors_level1` - controls the selection of satellites for preprocessing. This parameter should follow the FORCE notation for level 1 processing of satellites. Concretely, a string containing comma-separated satellite identifiers has to be supplied (e.g. `"LT04,LT05"` to include Landsat 4 and 5). Available options for satellite identifiers are: + +- `"LT04"`: Landsat 4 TM +- `"LT05"`: Landsat 5 TM +- `"LE07"`: Landsat 7 ETM+ +- `"LC08"`: Landsat 8 OLI +- `"S2A"`: Sentinel-2A MSI +- `"S2B"`: Sentinel-2B MSI + +The second parameter - `sensors_level2` - controls the selection of satellites for the higher level processing steps. The parameter has to follow the FORCE notation for level 2 processing. In particular, a string containing space-separated satellite identifiers has to be supplied (e.g. `"LND04 LND05"` to include Landsat 4 and 5). Note that these identifiers differ from those used for the `sensors_level1` parameter. +More details on available satellite identifiers can be found [here](https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html), some common options include: + +- `"LND04"`: 6-band Landsat 4 TM +- `"LND05"`: 6-band Landsat 5 TM +- `"LND07"`: 6-band Landsat 7 ETM+ +- `"LND08/09"`: 6-band Landsat 8-9 OLI +- `"SEN2A"`: 10-band Sentinel-2A +- `"SEN2B"`: 10-band Sentinel-2B + +Note that the identifiers specified for both processing levels have to match the data made available to the workflow. In other words, satellite data for e.g. Landsat 5 can't be processed if it was not supplied using the `input` parameter. + +Both parameters can be passed as using: + +```bash +--sensors_level1 = '[preprocessing satellite identifier string]' +--sensors_level2 = '[higher level processing satellite identifier string]' +``` + +Note that both parameters are optional and are by default set to: `"LT04,LT05,LE07,S2A"` and `"LND04 LND05 LND07"`. Therefore, by default, the pipeline will use Landsat 4,5,7, and Sentinel 2 for preprocessing, while using Landsat 4,5 and 7 for higher level processing. + +### Resolution + +Resolution of satellite imagery defines the real size of a single pixel. As an example, a resolution of 30 meters indicates that a single pixel in the data covers a 30x30 meters square of the earths surface. Users can customize the resolution that FORCE should assume. This does not necessarily have to match the resolution of the supplied data. FORCE will treat imagery as having the specified resolution. However, passing a resolution not matching the satellite data might lead to unexpected results. Resolution is specified in meters. + +A custom resolution can be passed using: + +```bash +--resolution '[integer]' +``` + +The default value is 30, as most Landsat satellite natively provide this resolution. + +### Temporal extent + +In some scenarios, user may be interested to limit the temporal extent of analysis. To enables this, users can specify both start and end date in a string with this syntax: `'YYYY-MM-DD'`. + +Start and end date can be passed using: + +```bash +--start_date '[YYYY-MM-DD]' +--end_date '[YYYY-MM-DD]' +``` + +Default values are `'1984-01-01'` for the start date and `'2006-12-31'` for the end date. + +### Group size + +The `group_size` parameters can be ignored in most cases. It defines how many satellite scenes are processed together. The parameters is used to balance the tradeoff between I/O and computational capacities on individual compute nodes. By default, `group_size` is set to 100. + +The group size can be passed using: + +```bash +--group_size '[integer]' +``` + +### Higher level processing configuration + +During the higher level processing stage, time series analyses of different satellite bands and indexes is performed. The concrete bands and indexes can be defined using the `indexes` parameter. Spectral unmixing is performed in any case. Thus, passing an empty `indexes` parameter will restrict time series analyses to the results of spectral unmixing. All available indexes can be found [here](https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html) above the `INDEX` parameter. The band/index codes need to be passed in a space-separated string. The default value, `indexes = "NDVI BLUE GREEN RED NIR SWIR1 SWIR2"`, enables time series analyses for the NDVI index and the blue, green, red, near-infrared and both shortwave infrared bands. Note that indexes are usually computed based on certain bands. If these bands are not present in the preprocessed data, these indexes can not be computed. + +The bands and indexes can be passed using: + +```bash +--indexes '[index-string]' +``` + +In so cases, it may be desirable to analyze the the individual images in a time series. To enable such analysis, the parameter `return_tss` can be used. If set to `true`, the pipeline will return time series stacks for each tile and band combination. The option is disabled by default to reduce the output size. + +The time series stack output can be enabled using: + +```bash +--return_tss '[boolean]' +``` + +### Visualization + +The workflow provides two types of results visualization and aggregation. The fine grained mosaic visualization contains all time series analyses results for all tiles in the original resolution. Pyramid visualizations present a broad overview of the same data but at a lower resolution. Both visualizations can be enabled or disabled using the parameters `mosaic_visualization` and `pyramid_visualization`. By default, both visualization methods are enabled. Note that the mosaic visualization is required to be enabled when using the `test` and `test_full` profiles to allow the pipeline to check the correctness of its results (this is the default behavior, make sure to not disable mosaic when using test profiles) . + +The visualizations can be enabled using: + +```bash +mosaic_visualization = '[boolean]' +pyramid_visualization = '[boolean]' +``` + +### FORCE configuration + +FORCE supports parallel computations. Users can specify the number of threads FORCE can spawn for a single preprocessing, or higher level processing process. This is archived through the `force_threads` parameter. + +The number of threads can be passed using: + +```bash +--force_threads '[integer]' +``` -An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. +The default value is 2. ## Running the pipeline The typical command for running the pipeline is as follows: ```bash -nextflow run nf-core/rangeland --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker +nextflow run nf-core/rangeland --input --dem --wvdb --data_cube --aoi --endmember --outdir -profile docker ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -88,9 +334,13 @@ nextflow run nf-core/rangeland -profile docker -params-file params.yaml with: ```yaml title="params.yaml" -input: './samplesheet.csv' +input: '' +dem: '' +wvdb: '' +data_cube: '' +aoi: '' +endmember: '' outdir: './results/' -genome: 'GRCh37' <...> ``` diff --git a/main.nf b/main.nf index 3650120..57a1b2c 100644 --- a/main.nf +++ b/main.nf @@ -29,17 +29,13 @@ include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_rang // workflow NFCORE_RANGELAND { - take: - samplesheet // channel: samplesheet read in from --input - main: // // WORKFLOW: Run pipeline // - RANGELAND ( - samplesheet - ) + RANGELAND () + emit: multiqc_report = RANGELAND.out.multiqc_report // channel: /path/to/multiqc_report.html } @@ -67,9 +63,8 @@ workflow { // // WORKFLOW: Run main workflow // - NFCORE_RANGELAND ( - PIPELINE_INITIALISATION.out.samplesheet - ) + NFCORE_RANGELAND () + // // SUBWORKFLOW: Run completion tasks // diff --git a/modules.json b/modules.json index c4d4381..5892af0 100644 --- a/modules.json +++ b/modules.json @@ -5,14 +5,14 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { - "fastqc": { + "multiqc": { "branch": "master", - "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", + "git_sha": "cf17ca47590cc578dfb47db1c2a44ef86f89976d", "installed_by": ["modules"] }, - "multiqc": { + "untar": { "branch": "master", - "git_sha": "cf17ca47590cc578dfb47db1c2a44ef86f89976d", + "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", "installed_by": ["modules"] } } diff --git a/modules/local/check_results.nf b/modules/local/check_results.nf new file mode 100644 index 0000000..cb57366 --- /dev/null +++ b/modules/local/check_results.nf @@ -0,0 +1,41 @@ +nextflow.enable.dsl = 2 + +process CHECK_RESULTS { + + label 'process_low' + + container 'docker.io/rocker/geospatial:4.3.1' + + input: + path{ "trend/?/*" } + path woody_change_ref + path woody_yoc_ref + path herbaceous_change_ref + path herbaceous_yoc_ref + path peak_change_ref + path peak_yoc_ref + + output: + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + files=`find ./trend/ -maxdepth 1 -mindepth 1 -type d` + for path in \$files; do + mkdir -p trend/\$(ls \$path) + cp \$path/*/* trend/\$(ls \$path)/ + rm \$path -r + done; + test.R trend/mosaic $woody_change_ref $woody_yoc_ref $herbaceous_change_ref $herbaceous_yoc_ref $peak_change_ref $peak_yoc_ref + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + r-base: \$(echo \$(R --version 2>&1) | sed 's/^.*R version //; s/ .*\$//') + terra: \$(Rscript -e "library(terra); cat(as.character(packageVersion('terra')))") + END_VERSIONS + """ + +} diff --git a/modules/local/check_results_full.nf b/modules/local/check_results_full.nf new file mode 100644 index 0000000..c57175a --- /dev/null +++ b/modules/local/check_results_full.nf @@ -0,0 +1,36 @@ +nextflow.enable.dsl = 2 + +process CHECK_RESULTS_FULL { + + label 'process_low' + + container 'docker.io/rocker/geospatial:4.3.1' + + input: + path{ "trend/?/*" } + path reference + + output: + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + files=`find ./trend/ -maxdepth 1 -mindepth 1 -type d` + for path in \$files; do + mkdir -p trend/\$(ls \$path) + cp \$path/*/* trend/\$(ls \$path)/ + rm \$path -r + done; + test.R trend/mosaic $reference + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + r-base: \$(echo \$(R --version 2>&1) | sed 's/^.*R version //; s/ .*\$//') + terra: \$(Rscript -e "library(terra); cat(as.character(packageVersion('terra')))") + END_VERSIONS + """ + +} diff --git a/modules/local/force-generate_analysis_mask.nf b/modules/local/force-generate_analysis_mask.nf new file mode 100644 index 0000000..b5ee8b4 --- /dev/null +++ b/modules/local/force-generate_analysis_mask.nf @@ -0,0 +1,31 @@ +nextflow.enable.dsl = 2 + +process FORCE_GENERATE_ANALYSIS_MASK{ + + label 'process_single' + + container "docker.io/davidfrantz/force:3.7.10" + + input: + path aoi + path 'mask/datacube-definition.prj' + + output: + //Mask for whole region + path 'mask/*/*.tif', emit: masks + path "versions.yml", emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + force-cube -o mask/ -s $params.resolution $aoi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + force: \$(force -v | sed 's/.*: //') + END_VERSIONS + """ + +} diff --git a/modules/local/force-generate_tile_allow_list.nf b/modules/local/force-generate_tile_allow_list.nf new file mode 100644 index 0000000..4ea0e19 --- /dev/null +++ b/modules/local/force-generate_tile_allow_list.nf @@ -0,0 +1,32 @@ +nextflow.enable.dsl = 2 + +process FORCE_GENERATE_TILE_ALLOW_LIST{ + + label 'process_single' + + container "docker.io/davidfrantz/force:3.7.10" + + input: + path aoi + path 'tmp/datacube-definition.prj' + + output: + //Tile allow for this image + path 'tile_allow.txt', emit: tile_allow + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + force-tile-extent $aoi tmp/ tile_allow.txt + rm -r tmp + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + force: \$(force -v | sed 's/.*: //') + END_VERSIONS + """ + +} diff --git a/modules/local/force-higher_level.nf b/modules/local/force-higher_level.nf new file mode 100644 index 0000000..c91d9b4 --- /dev/null +++ b/modules/local/force-higher_level.nf @@ -0,0 +1,46 @@ +nextflow.enable.dsl = 2 + +process FORCE_HIGHER_LEVEL { + + label 'process_medium' + + container "docker.io/davidfrantz/force:3.7.10" + tag { tile } + + input: + tuple val(tile), path(config), path(ard), path(aoi), path (datacube), path (endmember) + + output: + path 'trend/*.tif*', optional:true, emit: trend_files + path "versions.yml", emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + PARAM=$config + + mkdir trend + + # set provenance + mkdir prov + sed -i "/^DIR_PROVENANCE /c\\DIR_PROVENANCE = prov/" \$PARAM + + + force-higher-level \$PARAM + + #Rename files: /trend// to _, otherwise we can not reextract the tile name later + results=`find trend -name '*.tif*'` + for path in \$results; do + mv \$path \${path%/*}_\${path##*/} + done; + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + force: \$(force -v | sed 's/.*: //') + END_VERSIONS + """ + +} + diff --git a/modules/local/force-mosaic.nf b/modules/local/force-mosaic.nf new file mode 100644 index 0000000..2465b07 --- /dev/null +++ b/modules/local/force-mosaic.nf @@ -0,0 +1,38 @@ +nextflow.enable.dsl = 2 + +process FORCE_MOSAIC{ + + label 'process_low' + + tag { product } + container "docker.io/davidfrantz/force:3.7.10" + + input: + tuple val(product), path('trend/*') + path 'trend/datacube-definition.prj' + + output: + tuple val(product), path('trend/*'), emit: trend_files + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + #Move files from trend/_ to trend// + results=`find trend/*.tif*` + for path in \$results; do + mkdir -p \${path%_$product*} + mv \$path \${path%_$product*}/${product}.\${path#*.} + done; + + force-mosaic trend/ + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + force: \$(force -v | sed 's/.*: //') + END_VERSIONS + """ + +} diff --git a/modules/local/force-preprocess.nf b/modules/local/force-preprocess.nf new file mode 100644 index 0000000..c28af6a --- /dev/null +++ b/modules/local/force-preprocess.nf @@ -0,0 +1,50 @@ +nextflow.enable.dsl=2 + +process FORCE_PREPROCESS { + + label 'process_medium' + tag { data.simpleName } + + container "docker.io/davidfrantz/force:3.7.10" + + input: + tuple path(conf), path(data), path(cube), path(tile), path(dem), path(wvdb) + + output: + path "**/*BOA.tif", optional:true, emit: boa_tiles + path "**/*QAI.tif", optional:true, emit: qai_tiles + path "*.log" , emit: log + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + PARAM=$conf + + # make directories for force output + mkdir level2_ard + mkdir level2_log + mkdir level2_tmp + mkdir level2_prov + + + # set output directories in parameter file + sed -i "/^DIR_LEVEL2 /c\\DIR_LEVEL2 = level2_ard/" \$PARAM + sed -i "/^DIR_LOG /c\\DIR_LOG = level2_log/" \$PARAM + sed -i "/^DIR_TEMP /c\\DIR_TEMP = level2_tmp/" \$PARAM + sed -i "/^DIR_PROVENANCE /c\\DIR_PROVENANCE = level2_prov/" \$PARAM + + FILEPATH=$data + BASE=\$(basename $data) + force-l2ps \$FILEPATH \$PARAM > level2_log\$BASE.log + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + force: \$(force -v | sed 's/.*: //') + END_VERSIONS + """ + + +} diff --git a/modules/local/force-pyramid.nf b/modules/local/force-pyramid.nf new file mode 100644 index 0000000..e6eb9c6 --- /dev/null +++ b/modules/local/force-pyramid.nf @@ -0,0 +1,33 @@ +nextflow.enable.dsl = 2 + +process FORCE_PYRAMID { + + label 'process_low' + tag { tile } + + container "docker.io/davidfrantz/force:3.7.10" + + input: + tuple val(tile), path(image) + + output: + path('**') , emit: trends + path "versions.yml", emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + files="*.tif" + for file in \$files; do + force-pyramid \$file + done; + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + force: \$(force -v | sed 's/.*: //') + END_VERSIONS + """ + +} diff --git a/modules/local/higher_level_force_config.nf b/modules/local/higher_level_force_config.nf new file mode 100644 index 0000000..43ffeef --- /dev/null +++ b/modules/local/higher_level_force_config.nf @@ -0,0 +1,83 @@ +nextflow.enable.dsl = 2 + +process HIGHER_LEVEL_CONFIG { + + label 'process_single' + tag { tile } + + container "docker.io/davidfrantz/force:3.7.10" + + input: + tuple val(tile), path("ard/${tile}/*"), path("ard/${tile}/*"), path("mask/${tile}/aoi.tif") + path 'ard/datacube-definition.prj' + path endmember + + output: + tuple val (tile), path("trend_${tile}.prm"), path("ard/", includeInputs: true), path("mask/", includeInputs: true), path('ard/datacube-definition.prj', includeInputs: true), path(endmember, includeInputs: true), emit: higher_level_configs_and_data + path "versions.yml", emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + # generate parameterfile from scratch + force-parameter -c ./trend_${tile}.prm TSA + PARAM=trend_"$tile".prm + + # set parameters + + #Replace pathes + sed -i "/^DIR_LOWER /c\\DIR_LOWER = ard/" \$PARAM + sed -i "/^DIR_HIGHER /c\\DIR_HIGHER = trend/" \$PARAM + sed -i "/^DIR_MASK /c\\DIR_MASK = mask/" \$PARAM + sed -i "/^BASE_MASK /c\\BASE_MASK = aoi.tif" \$PARAM + sed -i "/^FILE_ENDMEM /c\\FILE_ENDMEM = $endmember" \$PARAM + + # threading + sed -i "/^NTHREAD_READ /c\\NTHREAD_READ = 1" \$PARAM # might need some modification + sed -i "/^NTHREAD_COMPUTE /c\\NTHREAD_COMPUTE = $params.force_threads" \$PARAM # might need some modification + sed -i "/^NTHREAD_WRITE /c\\NTHREAD_WRITE = 1" \$PARAM # might need some modification + + + # replace Tile to process + TILE="$tile" + X=\${TILE:1:4} + Y=\${TILE:7:11} + sed -i "/^X_TILE_RANGE /c\\X_TILE_RANGE = \$X \$X" \$PARAM + sed -i "/^Y_TILE_RANGE /c\\Y_TILE_RANGE = \$Y \$Y" \$PARAM + + # resolution + sed -i "/^RESOLUTION /c\\RESOLUTION = $params.resolution" \$PARAM + + + # sensors + sed -i "/^SENSORS /c\\SENSORS = $params.sensors_level2" \$PARAM + + + # date range + sed -i "/^DATE_RANGE /c\\DATE_RANGE = $params.start_date $params.end_date" \$PARAM + + + # spectral index + sed -i "/^INDEX /c\\INDEX = SMA $params.indexes" \$PARAM + ${ params.return_tss ? 'sed -i "/^OUTPUT_TSS /c\\OUTPUT_TSS = TRUE" \$PARAM' : '' } + + # interpolation + sed -i "/^INT_DAY /c\\INT_DAY = 8" \$PARAM + sed -i "/^OUTPUT_TSI /c\\OUTPUT_TSI = TRUE" \$PARAM + + # polar metrics + sed -i "/^POL /c\\POL = VPS VBL VSA" \$PARAM + sed -i "/^OUTPUT_POL /c\\OUTPUT_POL = TRUE" \$PARAM + sed -i "/^OUTPUT_TRO /c\\OUTPUT_TRO = TRUE" \$PARAM + sed -i "/^OUTPUT_CAO /c\\OUTPUT_CAO = TRUE" \$PARAM + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + force: \$(force -v | sed 's/.*: //') + END_VERSIONS + """ + +} + diff --git a/modules/local/merge.nf b/modules/local/merge.nf new file mode 100644 index 0000000..55b0d52 --- /dev/null +++ b/modules/local/merge.nf @@ -0,0 +1,56 @@ +nextflow.enable.dsl = 2 + +process MERGE { + + label 'process_low' + tag { id } + + container "docker.io/davidfrantz/force:3.7.10" + + input: + val (data_type) // defines whether qai or boa is merged + tuple val(id), path('input/?/*') + path cube + + output: + tuple val(id), path("*.tif"), emit: tiles_merged + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + files=`find -L input/ -type f -printf "%f\\n" | sort | uniq` + numberFiles=`echo \$files | wc -w` + currentFile=0 + + for file in \$files + do + currentFile=\$((currentFile+1)) + echo "Merging \$file (\$currentFile of \$numberFiles)" + + onefile=`ls -- */*/\${file} | head -1` + + #merge together + matchingFiles=`ls -- */*/\${file}` + if [ "$data_type" = "boa" ]; then + merge_boa.r \$file \${matchingFiles} + elif [ "$data_type" = "qai" ]; then + merge_qai.r \$file \${matchingFiles} + fi + + #apply meta + force-mdcp \$onefile \$file + + done; + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + force: \$(force -v | sed 's/.*: //') + r-base: \$(echo \$(R --version 2>&1) | sed 's/^.*R version //; s/ .*\$//') + raster: \$(Rscript -e "library(raster); cat(as.character(packageVersion('raster')))") + END_VERSIONS + """ + +} diff --git a/modules/local/preprocess_force_config.nf b/modules/local/preprocess_force_config.nf new file mode 100644 index 0000000..e759ac8 --- /dev/null +++ b/modules/local/preprocess_force_config.nf @@ -0,0 +1,60 @@ +nextflow.enable.dsl = 2 + +process PREPROCESS_CONFIG { + + label 'process_single' + tag { data.simpleName } + + container "docker.io/davidfrantz/force:3.7.10" + + input: + path data + path cube + path tile + path dem + path wvdb + + output: + tuple path("*.prm"), path(data), path(cube), path(tile), path(dem), path(wvdb), emit: preprocess_config_and_data + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + BASE=\$(basename $data) + + # generate parameterfile from scratch + force-parameter -c ./preprocess_${tile}.prm LEVEL2 + PARAM=\$BASE.prm + mv *.prm \$PARAM + + # read grid definition + CRS=\$(sed '1q;d' $cube) + ORIGINX=\$(sed '2q;d' $cube) + ORIGINY=\$(sed '3q;d' $cube) + TILESIZE=\$(sed '6q;d' $cube) + BLOCKSIZE=\$(sed '7q;d' $cube) + + # get dem vrt file + dem_file=\$(find $dem/ -type f -name "*.vrt" -print | head -n 1) + + # set parameters + sed -i "/^FILE_DEM /c\\FILE_DEM = \$dem_file" \$PARAM + sed -i "/^DIR_WVPLUT /c\\DIR_WVPLUT = $wvdb" \$PARAM + sed -i "/^FILE_TILE /c\\FILE_TILE = $tile" \$PARAM + sed -i "/^TILE_SIZE /c\\TILE_SIZE = \$TILESIZE" \$PARAM + sed -i "/^BLOCK_SIZE /c\\BLOCK_SIZE = \$BLOCKSIZE" \$PARAM + sed -i "/^ORIGIN_LON /c\\ORIGIN_LON = \$ORIGINX" \$PARAM + sed -i "/^ORIGIN_LAT /c\\ORIGIN_LAT = \$ORIGINY" \$PARAM + sed -i "/^PROJECTION /c\\PROJECTION = \$CRS" \$PARAM + sed -i "/^NTHREAD /c\\NTHREAD = $params.force_threads" \$PARAM + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + force: \$(force -v | sed 's/.*: //') + END_VERSIONS + """ + +} diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml deleted file mode 100644 index 691d4c7..0000000 --- a/modules/nf-core/fastqc/environment.yml +++ /dev/null @@ -1,5 +0,0 @@ -channels: - - conda-forge - - bioconda -dependencies: - - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf deleted file mode 100644 index d8989f4..0000000 --- a/modules/nf-core/fastqc/main.nf +++ /dev/null @@ -1,64 +0,0 @@ -process FASTQC { - tag "$meta.id" - label 'process_medium' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : - 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" - - input: - tuple val(meta), path(reads) - - output: - tuple val(meta), path("*.html"), emit: html - tuple val(meta), path("*.zip") , emit: zip - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - // Make list of old name and new name pairs to use for renaming in the bash while loop - def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } - def rename_to = old_new_pairs*.join(' ').join(' ') - def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') - - // The total amount of allocated RAM by FastQC is equal to the number of threads defined (--threads) time the amount of RAM defined (--memory) - // https://github.com/s-andrews/FastQC/blob/1faeea0412093224d7f6a07f777fad60a5650795/fastqc#L211-L222 - // Dividing the task.memory by task.cpu allows to stick to requested amount of RAM in the label - def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') / task.cpus - // FastQC memory value allowed range (100 - 10000) - def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb) - - """ - printf "%s %s\\n" $rename_to | while read old_name new_name; do - [ -f "\${new_name}" ] || ln -s \$old_name \$new_name - done - - fastqc \\ - $args \\ - --threads $task.cpus \\ - --memory $fastqc_memory \\ - $renamed_files - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.html - touch ${prefix}.zip - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) - END_VERSIONS - """ -} diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml deleted file mode 100644 index 4827da7..0000000 --- a/modules/nf-core/fastqc/meta.yml +++ /dev/null @@ -1,66 +0,0 @@ -name: fastqc -description: Run FastQC on sequenced reads -keywords: - - quality control - - qc - - adapters - - fastq -tools: - - fastqc: - description: | - FastQC gives general quality metrics about your reads. - It provides information about the quality score distribution - across your reads, the per base sequence content (%A/C/G/T). - You get information about adapter contamination and other - overrepresented sequences. - homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ - documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ - licence: ["GPL-2.0-only"] - identifier: biotools:fastqc -input: - - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. -output: - - html: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - "*.html": - type: file - description: FastQC report - pattern: "*_{fastqc.html}" - - zip: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - "*.zip": - type: file - description: FastQC report archive - pattern: "*_{fastqc.zip}" - - versions: - - versions.yml: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" - - "@ewels" - - "@FelixKrueger" -maintainers: - - "@drpatelh" - - "@grst" - - "@ewels" - - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test deleted file mode 100644 index e9d79a0..0000000 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ /dev/null @@ -1,309 +0,0 @@ -nextflow_process { - - name "Test Process FASTQC" - script "../main.nf" - process "FASTQC" - - tag "modules" - tag "modules_nfcore" - tag "fastqc" - - test("sarscov2 single-end [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [ id: 'test', single_end:true ], - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. - // looks like this:
    Mon 2 Oct 2023
    test.gz
    - // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("    ") }, - { assert snapshot(process.out.versions).match() } - ) - } - } - - test("sarscov2 paired-end [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("") }, - { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert snapshot(process.out.versions).match() } - ) - } - } - - test("sarscov2 interleaved [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match() } - ) - } - } - - test("sarscov2 paired-end [bam]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match() } - ) - } - } - - test("sarscov2 multiple [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, - { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, - { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("") }, - { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert path(process.out.html[0][1][2]).text.contains("") }, - { assert path(process.out.html[0][1][3]).text.contains("") }, - { assert snapshot(process.out.versions).match() } - ) - } - } - - test("sarscov2 custom_prefix") { - - when { - process { - """ - input[0] = Channel.of([ - [ id:'mysample', single_end:true ], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match() } - ) - } - } - - test("sarscov2 single-end [fastq] - stub") { - - options "-stub" - when { - process { - """ - input[0] = Channel.of([ - [ id: 'test', single_end:true ], - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert snapshot(process.out).match() } - ) - } - } - - test("sarscov2 paired-end [fastq] - stub") { - - options "-stub" - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert snapshot(process.out).match() } - ) - } - } - - test("sarscov2 interleaved [fastq] - stub") { - - options "-stub" - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert snapshot(process.out).match() } - ) - } - } - - test("sarscov2 paired-end [bam] - stub") { - - options "-stub" - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert snapshot(process.out).match() } - ) - } - } - - test("sarscov2 multiple [fastq] - stub") { - - options "-stub" - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert snapshot(process.out).match() } - ) - } - } - - test("sarscov2 custom_prefix - stub") { - - options "-stub" - when { - process { - """ - input[0] = Channel.of([ - [ id:'mysample', single_end:true ], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert snapshot(process.out).match() } - ) - } - } -} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap deleted file mode 100644 index d5db309..0000000 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ /dev/null @@ -1,392 +0,0 @@ -{ - "sarscov2 custom_prefix": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.9.0", - "nextflow": "24.04.3" - }, - "timestamp": "2024-07-22T11:02:16.374038" - }, - "sarscov2 single-end [fastq] - stub": { - "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": true - }, - "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "1": [ - [ - { - "id": "test", - "single_end": true - }, - "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" - 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}, - "sarscov2 multiple [fastq] - stub": { - "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": false - }, - "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "1": [ - [ - { - "id": "test", - "single_end": false - }, - "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "2": [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ], - "html": [ - [ - { - "id": "test", - "single_end": false - }, - "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "versions": [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ], - "zip": [ - [ - { - "id": "test", - "single_end": false - }, - "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ] - } - ], - "meta": { - "nf-test": "0.9.0", - "nextflow": "24.04.3" - }, - "timestamp": "2024-07-22T11:03:02.304411" - }, - "sarscov2 single-end [fastq]": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.9.0", - "nextflow": "24.04.3" - }, - "timestamp": "2024-07-22T11:01:19.095607" - }, - "sarscov2 interleaved [fastq] - stub": { - "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": false - }, - "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "1": [ - [ - { - "id": "test", - "single_end": false - }, - "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "2": [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ], - "html": [ - [ - { - "id": "test", - "single_end": false - }, - "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "versions": [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ], - "zip": [ - [ - { - "id": "test", - "single_end": false - }, - "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ] - } - ], - "meta": { - "nf-test": "0.9.0", - "nextflow": "24.04.3" - }, - "timestamp": "2024-07-22T11:02:44.640184" - }, - "sarscov2 paired-end [bam] - stub": { - "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": false - }, - "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "1": [ - [ - { - "id": "test", - "single_end": false - }, - "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "2": [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ], - "html": [ - [ - { - "id": "test", - "single_end": false - }, - "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "versions": [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ], - "zip": [ - [ - { - "id": "test", - "single_end": false - }, - "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ] - } - ], - "meta": { - "nf-test": "0.9.0", - "nextflow": "24.04.3" - }, - "timestamp": "2024-07-22T11:02:53.550742" - } -} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml deleted file mode 100644 index 7834294..0000000 --- a/modules/nf-core/fastqc/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -fastqc: - - modules/nf-core/fastqc/** diff --git a/modules/nf-core/untar/main.nf b/modules/nf-core/untar/main.nf new file mode 100644 index 0000000..61461c3 --- /dev/null +++ b/modules/nf-core/untar/main.nf @@ -0,0 +1,63 @@ +process UNTAR { + tag "$archive" + label 'process_single' + + conda "conda-forge::sed=4.7 conda-forge::grep=3.11 conda-forge::tar=1.34" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : + 'nf-core/ubuntu:20.04' }" + + input: + tuple val(meta), path(archive) + + output: + tuple val(meta), path("$prefix"), emit: untar + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + prefix = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.baseName.toString().replaceFirst(/\.tar$/, "")) + + """ + mkdir $prefix + + ## Ensures --strip-components only applied when top level of tar contents is a directory + ## If just files or multiple directories, place all in prefix + if [[ \$(tar -taf ${archive} | grep -o -P "^.*?\\/" | uniq | wc -l) -eq 1 ]]; then + tar \\ + -C $prefix --strip-components 1 \\ + -xavf \\ + $args \\ + $archive \\ + $args2 + else + tar \\ + -C $prefix \\ + -xavf \\ + $args \\ + $archive \\ + $args2 + fi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//') + END_VERSIONS + """ + + stub: + prefix = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.toString().replaceFirst(/\.[^\.]+(.gz)?$/, "")) + """ + mkdir $prefix + touch ${prefix}/file.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/untar/meta.yml b/modules/nf-core/untar/meta.yml new file mode 100644 index 0000000..a9a2110 --- /dev/null +++ b/modules/nf-core/untar/meta.yml @@ -0,0 +1,46 @@ +name: untar +description: Extract files. +keywords: + - untar + - uncompress + - extract +tools: + - untar: + description: | + Extract tar.gz files. + documentation: https://www.gnu.org/software/tar/manual/ + licence: ["GPL-3.0-or-later"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - archive: + type: file + description: File to be untar + pattern: "*.{tar}.{gz}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - untar: + type: directory + description: Directory containing contents of archive + pattern: "*/" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" + - "@matthdsm" + - "@jfy133" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@matthdsm" + - "@jfy133" diff --git a/nextflow.config b/nextflow.config index 5d0e4ad..d928bab 100644 --- a/nextflow.config +++ b/nextflow.config @@ -9,17 +9,44 @@ // Global default params, used in configs params { - // TODO nf-core: Specify your pipeline's command line flags // Input options - input = null + input = null + dem = null + wvdb = null + data_cube = null + aoi = null + endmember = null - + input_tar = false + dem_tar = false + wvdb_tar = false + + // Remote sensing imagery parameters + sensors_level1 = "LT04,LT05,LE07,S2A" + sensors_level2 = "LND04 LND05 LND07" + resolution = 30 + start_date = "1984-01-01" + end_date = "2006-12-31" + + // Workflow configuration + group_size = 100 + + // FORCE + force_threads = 2 + + // Higher Level processing configuration + indexes = "NDVI BLUE GREEN RED NIR SWIR1 SWIR2" + return_tss = false + + // Visualization + mosaic_visualization = true + pyramid_visualization = true // MultiQC options - multiqc_config = null - multiqc_title = null - multiqc_logo = null - max_multiqc_email_size = '25.MB' + multiqc_config = null + multiqc_title = null + multiqc_logo = null + max_multiqc_email_size = '25.MB' multiqc_methods_description = null // Boilerplate options @@ -80,14 +107,16 @@ profiles { apptainer.enabled = false } docker { - docker.enabled = true - conda.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false - docker.runOptions = '-u $(id -u):$(id -g)' + docker.enabled = true + docker.userEmulation = true + conda.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false + docker.fixOwnership = true + docker.runOptions = '-u $(id -u):$(id -g)' } arm { docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' @@ -160,7 +189,7 @@ includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${pa // Load nf-core/rangeland custom profiles from different institutions. // TODO nf-core: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs -// includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/rangeland.config" : "/dev/null" +includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/rangeland.config" : "/dev/null" // Set default registry for Apptainer, Docker, Podman, Charliecloud and Singularity independent of -profile // Will not be used unless Apptainer / Docker / Podman / Charliecloud / Singularity are enabled diff --git a/nextflow_schema.json b/nextflow_schema.json index 07f5b59..90d0a7a 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,18 +10,62 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "outdir"], + "required": ["input", "dem", "wvdb", "data_cube", "aoi", "endmember", "outdir"], "properties": { "input": { "type": "string", - "format": "file-path", - "exists": true, - "schema": "assets/schema_input.json", - "mimetype": "text/csv", - "pattern": "^\\S+\\.csv$", - "description": "Path to comma-separated file containing information about the samples in the experiment.", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/rangeland/usage#samplesheet-input).", - "fa_icon": "fas fa-file-csv" + "fa_icon": "fas fa-satellite", + "help_text": "Set this to the root directory of all satellite imagery. Directory structure should match format of data downloaded with '$ force-level1-csd\n' For concrete directory structure see ./docs/usage.md ", + "description": "Root directory of all sattelite imagery.", + "mimetype": "application/x-tar" + }, + "input_tar": { + "type": "boolean", + "fa_icon": "fas fa-archive", + "description": "Indicates whether input is a tar archive.", + "help_text": "Set to true if input is a tar archive. The workflow will extract it then." + }, + "dem": { + "type": "string", + "help_text": "Path to directory containing a subdirectory with tile-wise digital elevation files (.tif) and a virtual dataset file (.vrt file) comprising all tile-wise files from the subdirectory. \n\n\n", + "fa_icon": "fas fa-mountain", + "description": "Digital elevation model." + }, + "dem_tar": { + "type": "boolean", + "fa_icon": "fas fa-archive", + "description": "Indicates whether dem is a tar archive.", + "help_text": "Set to true if dem is a tar archive. The workflow will extract it then." + }, + "wvdb": { + "type": "string", + "fa_icon": "fas fa-burn", + "description": "Water vapor dataset.", + "help_text": "Directory containg a number text files describing global water vapor data at different timestamps, and a coordinate order (.coo-)file containig the reference system of the water vapor data." + }, + "wvdb_tar": { + "type": "boolean", + "fa_icon": "fas fa-archive", + "description": "Indicates whether wvdb is a tar archive.", + "help_text": "Set to true if wvdb is a tar archive. The workflow will extract it then." + }, + "data_cube": { + "type": "string", + "description": "Datacube definition.", + "fa_icon": "fas fa-cube", + "help_text": "A single .prj file describing the projection and reference grid of the generated datacube." + }, + "aoi": { + "type": "string", + "fa_icon": "fas fa-chart-area", + "description": "Area of interest.", + "help_text": "A single vector file specifying the spacial extend of the targeted area." + }, + "endmember": { + "type": "string", + "fa_icon": "fas fa-chart-line", + "description": "Endmember definition.", + "help_text": "A single text file specifying where lines correspond to satellite bands and columns correspond to endmembers. Values correspond to reflectance values and are separated by spaces." }, "outdir": { "type": "string", @@ -43,6 +87,127 @@ } } }, + "remote_sensing_image_options": { + "title": "Remote sensing image options", + "type": "object", + "description": "Definition of satellite image parameters.", + "default": "", + "help_text": "These parameters are used to tell pipeline tools which data is expected, how this data has to be incorporated, and which parts of the imagery can be excluded (e.g. due to temporal mismatch). These parameters should be closely aligned with the provided input data.", + "properties": { + "sensors_level1": { + "type": "string", + "default": "LT04,LT05,LE07,S2A", + "fa_icon": "fas fa-satellite", + "description": "Satellites for which first level data should be processed.", + "help_text": "String containing comma-separated indicators of satellites in FORCE level 1format. Possible options: \n\"LT04\": Landsat 4 TM, \n\"LT05\": Landsat 5 TM, \n\"LE07\": Landsat 7 ETM+,\n\"LC08\": Landsat 8 OLI,\n\"S2A\": Sentinel-2A MSI,\n\"S2B\": Sentinel-2B MSI" + }, + "sensors_level2": { + "type": "string", + "default": "LND04 LND05 LND07", + "fa_icon": "fas fa-satellite", + "description": "Satellites for which data should be incorporated into higher level processing.", + "help_text": "String containing space-separated indicators of satellites in FORCE level 2 format. Common options:\n\"LND04\": 6-band Landsat 4 TM, \n\"LND05\": 6-band Landsat 5 TM, \n\"LND07\": 6-band Landsat 7 ETM+,\n\"LND08/09\": 6-band Landsat 8-9 OLI, \n\"SEN2A\": 10-band Sentinel-2A, \n\"SEN2B\": 10-band Sentinel-2B,\nall options [here](https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html) \n" + }, + "start_date": { + "type": "string", + "default": "1984-01-01", + "fa_icon": "far fa-calendar-alt", + "description": "First day of interest.", + "help_text": "String with format: \"YYYY-MM-DD\"." + }, + "end_date": { + "type": "string", + "default": "2006-12-31", + "fa_icon": "far fa-calendar-alt", + "description": "Last day of interest.", + "help_text": "String with format: \"YYYY-MM-DD\"." + }, + "resolution": { + "type": "integer", + "default": 30, + "fa_icon": "fas fa-expand-arrows-alt", + "description": "Spatial resolution applied in analyses." + } + }, + "fa_icon": "fas fa-satellite" + }, + "higher_level_processing_modification": { + "title": "Higher level processing modification", + "type": "object", + "default": "", + "properties": { + "indexes": { + "type": "string", + "default": "NDVI BLUE GREEN RED NIR SWIR1 SWIR2", + "help_text": "Space-separated list of indexes and bands that should be considered in time series analyses. They are indicated by using their established abbreviations. The full list of available indexes is available at https://force-eo.readthedocs.io/en/latest/components/higher-level/tsa/param.html under the 'INDEX' parameter. Spectral unmixing is a special index and always activated.", + "description": "Select which bands and indexes should be considered in time series analyses.", + "fa_icon": "fas fa-satellite" + }, + "return_tss": { + "type": "boolean", + "description": "Should the full time series stack be returned.", + "help_text": "This parameter enables or disables the output of the time series stack for each tile and band/index. Here, each band shows the values of a single date.", + "fa_icon": "fas fa-layer-group" + } + }, + "description": "Modify higher level processing behaviour.", + "help_text": "These parameters can be used to modify the behaviour of the time series analyses process in higher level processing.", + "fa_icon": "fas fa-microchip" + }, + "visualization": { + "title": "Visualization", + "type": "object", + "description": "Enable or disable visualizations.", + "default": "", + "properties": { + "mosaic_visualization": { + "type": "boolean", + "default": true, + "description": "Whether mosaic visualization should be returned.", + "fa_icon": "fas fa-image" + }, + "pyramid_visualization": { + "type": "boolean", + "default": true, + "description": "Whether pyramid visualization should be returned.", + "fa_icon": "far fa-image" + } + }, + "fa_icon": "fas fa-images" + }, + "workflow_configuration": { + "title": "Workflow configuration", + "type": "object", + "description": "Parameters that configure workflow behavior not directly related to FORCE,", + "default": "", + "properties": { + "group_size": { + "type": "integer", + "default": 100, + "description": "Batch size of tiles considered for merging.", + "help_text": "Controls trade-off between parallelism and I/O load.\nHigher group_size -> More tiles merged in one process, less I/O load.\nLower group_size -> Less tiles merged in one process, more I/O load. ", + "fa_icon": "fas fa-layer-group" + } + }, + "help_text": "You can savely use the defaults.", + "fa_icon": "fas fa-project-diagram" + }, + "force_parameters": { + "title": "FORCE parameters", + "type": "object", + "description": "Parameters specific to the FORCE tool.", + "default": "", + "properties": { + "force_threads": { + "type": "integer", + "default": 2, + "description": "Number of threads spawned by FORCE for each higher-level or preprocessing task.", + "fa_icon": "fas fa-microchip" + } + }, + "fa_icon": "fas fa-terminal", + "help_text": "" + }, "institutional_config_options": { "title": "Institutional config options", "type": "object", @@ -187,7 +352,21 @@ { "$ref": "#/$defs/input_output_options" }, - + { + "$ref": "#/$defs/remote_sensing_image_options" + }, + { + "$ref": "#/$defs/higher_level_processing_modification" + }, + { + "$ref": "#/$defs/visualization" + }, + { + "$ref": "#/$defs/workflow_configuration" + }, + { + "$ref": "#/$defs/force_parameters" + }, { "$ref": "#/$defs/institutional_config_options" }, diff --git a/subworkflows/local/higher_level.nf b/subworkflows/local/higher_level.nf new file mode 100644 index 0000000..d1006ac --- /dev/null +++ b/subworkflows/local/higher_level.nf @@ -0,0 +1,51 @@ +nextflow.enable.dsl = 2 + +//inputs +include { HIGHER_LEVEL_CONFIG } from '../../modules/local/higher_level_force_config.nf' +include { FORCE_HIGHER_LEVEL } from '../../modules/local/force-higher_level.nf' +include { FORCE_MOSAIC } from '../../modules/local/force-mosaic.nf' +include { FORCE_PYRAMID } from '../../modules/local/force-pyramid.nf' + +workflow HIGHER_LEVEL { + + take: + tiles_and_masks + cube_file + endmember_file + + main: + + ch_versions = Channel.empty() + + // create configuration file for higher level processing + HIGHER_LEVEL_CONFIG( tiles_and_masks, cube_file, endmember_file ) + ch_versions = ch_versions.mix(HIGHER_LEVEL_CONFIG.out.versions.first()) + + // main processing + FORCE_HIGHER_LEVEL( HIGHER_LEVEL_CONFIG.out.higher_level_configs_and_data ) + ch_versions = ch_versions.mix(FORCE_HIGHER_LEVEL.out.versions.first()) + + + trend_files = FORCE_HIGHER_LEVEL.out.trend_files.flatten().map{ x -> [ x.simpleName.substring(12), x ] } + + trend_files_mosaic = trend_files.groupTuple() + + // visualizations + mosaic_files = Channel.empty() + if (params.mosaic_visualization) { + FORCE_MOSAIC( trend_files_mosaic, cube_file ) + mosaic_files = FORCE_MOSAIC.out.trend_files + ch_versions = ch_versions.mix(FORCE_MOSAIC.out.versions.first()) + } + + if (params.pyramid_visualization) { + FORCE_PYRAMID( trend_files.filter { it[1].name.endsWith('.tif') }.map { [ it[1].simpleName.substring(0,11), it[1] ] } .groupTuple() ) + ch_versions = ch_versions.mix(FORCE_PYRAMID.out.versions.first()) + } + + emit: + mosaic_files + + versions = ch_versions + +} diff --git a/subworkflows/local/preprocessing.nf b/subworkflows/local/preprocessing.nf new file mode 100644 index 0000000..89766c0 --- /dev/null +++ b/subworkflows/local/preprocessing.nf @@ -0,0 +1,83 @@ +nextflow.enable.dsl = 2 + +//includes +include { FORCE_GENERATE_TILE_ALLOW_LIST } from '../../modules/local/force-generate_tile_allow_list' +include { FORCE_GENERATE_ANALYSIS_MASK } from '../../modules/local/force-generate_analysis_mask' +include { PREPROCESS_CONFIG } from '../../modules/local/preprocess_force_config' +include { FORCE_PREPROCESS } from '../../modules/local/force-preprocess' +include { MERGE as MERGE_BOA; MERGE as MERGE_QAI } from '../../modules/local/merge' + +//Closure to extract the parent directory of a file +def extractDirectory = { it.parent.toString().substring(it.parent.toString().lastIndexOf('/') + 1 ) } + +workflow PREPROCESSING { + + take: + data + dem + wvdb + cube_file + aoi_file + + main: + + ch_versions = Channel.empty() + + FORCE_GENERATE_TILE_ALLOW_LIST( aoi_file, cube_file ) + ch_versions = ch_versions.mix(FORCE_GENERATE_TILE_ALLOW_LIST.out.versions) + + FORCE_GENERATE_ANALYSIS_MASK( aoi_file, cube_file ) + ch_versions = ch_versions.mix(FORCE_GENERATE_ANALYSIS_MASK.out.versions) + + //Group masks by tile + masks = FORCE_GENERATE_ANALYSIS_MASK.out.masks.flatten().map{ x -> [ extractDirectory(x), x ] } + + // Preprocessing configuration + PREPROCESS_CONFIG( data, cube_file, FORCE_GENERATE_TILE_ALLOW_LIST.out.tile_allow, dem, wvdb ) + ch_versions = ch_versions.mix(PREPROCESS_CONFIG.out.versions.first()) + + // Main preprocessing + FORCE_PREPROCESS( PREPROCESS_CONFIG.out.preprocess_config_and_data) + ch_versions = ch_versions.mix(FORCE_PREPROCESS.out.versions.first()) + + //Group by tile, date and sensor + boa_tiles = FORCE_PREPROCESS.out.boa_tiles.flatten().map{ [ "${extractDirectory(it)}_${it.simpleName}", it ] }.groupTuple() + qai_tiles = FORCE_PREPROCESS.out.qai_tiles.flatten().map{ [ "${extractDirectory(it)}_${it.simpleName}", it ] }.groupTuple() + + //Find tiles to merge + boa_tiles_to_merge = boa_tiles.filter{ x -> x[1].size() > 1 } + .map{ [ it[0].substring( 0, 11 ), it[1] ] } + //Sort to ensure the same groups if you use resume + .toSortedList{ a,b -> a[1][0].simpleName <=> b[1][0].simpleName } + .flatMap{it} + .groupTuple( remainder : true, size : params.group_size ).map{ [ it[0], it[1] .flatten() ] } + qai_tiles_to_merge = qai_tiles.filter{ x -> x[1].size() > 1 } + .map{ [ it[0].substring( 0, 11 ), it[1] ] } + //Sort to ensure the same groups if you use resume + .toSortedList{ a,b -> a[1][0].simpleName <=> b[1][0].simpleName } + .flatMap{it} + .groupTuple( remainder : true, size : params.group_size ).map{ [ it[0], it[1] .flatten() ] } + + //Find tiles with only one file + boa_tiles_done = boa_tiles.filter{ x -> x[1].size() == 1 }.map{ x -> [ x[0] .substring( 0, 11 ), x[1][0] ] } + qai_tiles_done = qai_tiles.filter{ x -> x[1].size() == 1 }.map{ x -> [ x[0] .substring( 0, 11 ), x[1][0] ] } + + MERGE_BOA( "boa", boa_tiles_to_merge, cube_file ) + ch_versions = ch_versions.mix(MERGE_BOA.out.versions.first()) + + MERGE_QAI( "qai", qai_tiles_to_merge, cube_file ) + ch_versions = ch_versions.mix(MERGE_QAI.out.versions.first()) + + //Concat merged list with single images, group by tile over time + boa_tiles = MERGE_BOA.out.tiles_merged + .concat( boa_tiles_done ).groupTuple() + .map { [it[0], it[1].flatten() ] } + qai_tiles = MERGE_QAI.out.tiles_merged + .concat( qai_tiles_done ).groupTuple() + .map { [it[0], it[1].flatten() ] } + + emit: + tiles_and_masks = boa_tiles.join( qai_tiles ).join( masks ) + versions = ch_versions + +} diff --git a/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf b/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf index 363f0e6..f7bd710 100644 --- a/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_rangeland_pipeline/main.nf @@ -63,32 +63,8 @@ workflow PIPELINE_INITIALISATION { nextflow_cli_args ) - // - // Create channel from input file provided through params.input - // - - Channel - .fromList(samplesheetToList(params.input, "${projectDir}/assets/schema_input.json")) - .map { - meta, fastq_1, fastq_2 -> - if (!fastq_2) { - return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] - } else { - return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] - } - } - .groupTuple() - .map { samplesheet -> - validateInputSamplesheet(samplesheet) - } - .map { - meta, fastqs -> - return [ meta, fastqs.flatten() ] - } - .set { ch_samplesheet } emit: - samplesheet = ch_samplesheet versions = ch_versions } @@ -163,13 +139,12 @@ def validateInputSamplesheet(input) { // Generate methods description for MultiQC // def toolCitationText() { - // TODO nf-core: Optionally add in-text citation tools to this list. // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", // Uncomment function in methodsDescriptionText to render in MultiQC report def citation_text = [ "Tools used in the workflow included:", - "FastQC (Andrews 2010),", "MultiQC (Ewels et al. 2016)", + "FORCE (Frantz 2019)", "." ].join(' ').trim() @@ -177,12 +152,11 @@ def toolCitationText() { } def toolBibliographyText() { - // TODO nf-core: Optionally add bibliographic entries to this list. // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", // Uncomment function in methodsDescriptionText to render in MultiQC report def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • ", + "
  • Frantz, D. (2019). FORCE—Landsat + Sentinel-2 Analysis Ready Data and Beyond. Remote Sensing, 11, 1124 doi: https://doi.org/10.3390/rs11091124
    • " ].join(' ').trim() return reference_text @@ -212,9 +186,8 @@ def methodsDescriptionText(mqc_methods_yaml) { meta["tool_citations"] = "" meta["tool_bibliography"] = "" - // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - // meta["tool_bibliography"] = toolBibliographyText() + meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + meta["tool_bibliography"] = toolBibliographyText() def methods_text = mqc_methods_yaml.text diff --git a/workflows/rangeland.nf b/workflows/rangeland.nf index 80929de..2b152a1 100644 --- a/workflows/rangeland.nf +++ b/workflows/rangeland.nf @@ -3,35 +3,157 @@ IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { FASTQC } from '../modules/nf-core/fastqc/main' + include { MULTIQC } from '../modules/nf-core/multiqc/main' include { paramsSummaryMap } from 'plugin/nf-schema' include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_rangeland_pipeline' +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT LOCAL MODULES/SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +// +// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules +// +include { PREPROCESSING } from '../subworkflows/local/preprocessing' +include { HIGHER_LEVEL } from '../subworkflows/local/higher_level' + +// +// MODULES +// + +include { CHECK_RESULTS } from '../modules/local/check_results' +include { CHECK_RESULTS_FULL } from '../modules/local/check_results_full' +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT NF-CORE MODULES/SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +// +// MODULE: Installed directly from nf-core/modules +// +include { UNTAR as UNTAR_INPUT; UNTAR as UNTAR_DEM; UNTAR as UNTAR_WVDB; UNTAR as UNTAR_REF } from '../modules/nf-core/untar/main' + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + HELPER FUNCTIONS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + + +// check wether provided input is within provided time range +def inRegion = input -> { + Integer date = input.simpleName.split("_")[3] as Integer + Integer start = params.start_date.replace('-','') as Integer + Integer end = params.end_date.replace('-','') as Integer + + return date >= start && date <= end +} + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ RUN MAIN WORKFLOW ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ + + workflow RANGELAND { - take: - ch_samplesheet // channel: samplesheet read in from --input main: ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() // - // MODULE: Run FastQC + // Stage and validate input files // - FASTQC ( - ch_samplesheet - ) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) - ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + data = null + dem = null + wvdb = null + cube_file = file( "$params.data_cube" ) + aoi_file = file( "$params.aoi" ) + endmember_file = file( "$params.endmember" ) + + // + // MODULE: untar + // + tar_versions = Channel.empty() + if (params.input_tar) { + UNTAR_INPUT([[:], params.input]) + base_path = UNTAR_INPUT.out.untar.map(it -> it[1]) + + data = base_path.map(it -> file("$it/*/*", type: 'dir')).flatten() + data = data.flatten().filter{ inRegion(it) } + + tar_versions = tar_versions.mix(UNTAR_INPUT.out.versions) + } else { + data = Channel.fromPath( "${params.input}/*/*", type: 'dir') .flatten() + data = data.flatten().filter{ inRegion(it) } + } + + if (params.dem_tar) { + UNTAR_DEM([[:], params.dem]) + dem = UNTAR_DEM.out.untar.map(it -> file(it[1])) + + tar_versions = tar_versions.mix(UNTAR_DEM.out.versions) + } else { + dem = file("$params.dem") + } + + if (params.wvdb_tar) { + UNTAR_WVDB([[:], params.wvdb]) + wvdb = UNTAR_WVDB.out.untar.map(it -> file(it[1])) + + tar_versions = tar_versions.mix(UNTAR_WVDB.out.versions) + } else { + wvdb = file("$params.wvdb") + } + + + // + // SUBWORKFLOW: Preprocess satellite imagery + // + PREPROCESSING(data, dem, wvdb, cube_file, aoi_file) + ch_versions = ch_versions.mix(PREPROCESSING.out.versions) + + // + // SUBWORKFLOW: Generate trend files and visualization + // + HIGHER_LEVEL(PREPROCESSING.out.tiles_and_masks, cube_file, endmember_file) + ch_versions = ch_versions.mix(HIGHER_LEVEL.out.versions) + + grouped_trend_data = HIGHER_LEVEL.out.mosaic_files.map{ it[1] }.flatten().buffer( size: Integer.MAX_VALUE, remainder: true ) + + // + // MODULE: Check results + // + if (params.config_profile_name == 'Test profile') { + woody_change_ref = file("$params.woody_change_ref") + woody_yoc_ref = file("$params.woody_yoc_ref") + herbaceous_change_ref = file("$params.herbaceous_change_ref") + herbaceous_yoc_ref = file("$params.herbaceous_yoc_ref") + peak_change_ref = file("$params.peak_change_ref") + peak_yoc_ref = file("$params.peak_yoc_ref") + + CHECK_RESULTS(grouped_trend_data, woody_change_ref, woody_yoc_ref, herbaceous_change_ref, herbaceous_yoc_ref, peak_change_ref, peak_yoc_ref) + ch_versions = ch_versions.mix(CHECK_RESULTS.out.versions) + } + + if (params.config_profile_name == 'Full test profile') { + UNTAR_REF([[:], params.reference]) + ref_path = UNTAR_REF.out.untar.map(it -> it[1]) + tar_versions.mix(UNTAR_REF.out.versions) + + CHECK_RESULTS_FULL(grouped_trend_data, ref_path) + ch_versions = ch_versions.mix(CHECK_RESULTS_FULL.out.versions) + } + + ch_versions = ch_versions.mix(tar_versions.first()) // // Collate and save software versions From 22166e97db3f589dcca5fa1944121dd63a9da556 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Fri, 25 Oct 2024 09:31:15 +0200 Subject: [PATCH 48/61] Updated input mimetype in schema --- nextflow_schema.json | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 1c863d7..25a6c91 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -17,7 +17,7 @@ "fa_icon": "fas fa-satellite", "help_text": "Set this to the root directory of all satellite imagery. Directory structure should match format of data downloaded with '$ force-level1-csd\n' For concrete directory structure see [usage documentation](https://nf-co.re/rangeland/docs/usage).\n\nAlternatively, a tarball can be supplied. The same directory structure needs to be in place in that tarball.", "description": "Root directory or tarball of all satellite imagery.", - "mimetype": "application/x-tar", + "mimetype": "image/tiff, application/x-tar", "format": "path" }, "dem": { @@ -339,7 +339,6 @@ { "$ref": "#/$defs/input_output_options" }, - { "$ref": "#/$defs/remote_sensing_image_options" }, From c822ba7ac33c6ac4760058220ba439ab3a2fa905 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Fri, 25 Oct 2024 11:00:19 +0200 Subject: [PATCH 49/61] Added missing fields to schema --- nextflow_schema.json | 12 ++++++++++-- 1 file changed, 10 insertions(+), 2 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 25a6c91..5829b02 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -15,6 +15,7 @@ "input": { "type": "string", "fa_icon": "fas fa-satellite", + "exists": true, "help_text": "Set this to the root directory of all satellite imagery. Directory structure should match format of data downloaded with '$ force-level1-csd\n' For concrete directory structure see [usage documentation](https://nf-co.re/rangeland/docs/usage).\n\nAlternatively, a tarball can be supplied. The same directory structure needs to be in place in that tarball.", "description": "Root directory or tarball of all satellite imagery.", "mimetype": "image/tiff, application/x-tar", @@ -24,12 +25,14 @@ "type": "string", "help_text": "Path to directory containing a subdirectory with tile-wise digital elevation files (`.tif`) and a virtual dataset file (`.vrt` file) comprising all tile-wise files from the subdirectory. See [usage documentation](https://nf-co.re/rangeland/docs/usage) for details regarding the required structure.\n\nAlternatively, a tarball can be supplied. The same directory structure needs to be in place in that tarball.\n\nAlternatively, a tarball can be supplied. The same directory structure needs to be in place in that tarball.", "fa_icon": "fas fa-mountain", + "exists": true, "description": "Digital elevation model.", "format": "path" }, "wvdb": { "type": "string", "fa_icon": "fas fa-burn", + "exists": true, "description": "Water vapor dataset.", "help_text": "Directory containing a number text files describing global water vapor data at different timestamps, and a coordinate order (`.coo`-)file containing the reference system of the water vapor data. See [usage documentation](https://nf-co.re/rangeland/docs/usage) for details regarding the required structure.\n\nAlternatively, a tarball can be supplied. The same directory structure needs to be in place in that tarball.", "format": "path" @@ -38,6 +41,7 @@ "type": "string", "description": "Datacube definition.", "fa_icon": "fas fa-cube", + "exists": true, "help_text": "A single `.prj` file describing the projection and reference grid of the generated datacube.", "pattern": "^\\S+\\.prj$", "format": "file-path" @@ -45,6 +49,7 @@ "aoi": { "type": "string", "fa_icon": "fas fa-chart-area", + "exists": true, "description": "Area of interest.", "help_text": "A single vector file specifying the spatial extend of the targeted area.", "pattern": "^\\S+\\.(gpkg|shp)$", @@ -53,6 +58,7 @@ "endmember": { "type": "string", "fa_icon": "fas fa-chart-line", + "exists": true, "description": "Endmember definition.", "help_text": "A single text file specifying where rows correspond to satellite bands and columns correspond to endmembers. Values correspond to reflectance values and are separated by spaces.", "pattern": "^\\S+\\.txt$", @@ -63,7 +69,8 @@ "type": "string", "format": "directory-path", "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", - "fa_icon": "fas fa-folder-open" + "fa_icon": "fas fa-folder-open", + "exists": true }, "email": { "type": "string", @@ -116,7 +123,8 @@ "type": "integer", "default": 30, "fa_icon": "fas fa-expand-arrows-alt", - "description": "Spatial resolution applied in analyses." + "description": "Spatial resolution applied in analyses.", + "minimum": 1 } }, "fa_icon": "fas fa-satellite" From 15e4c1ecf9d79d54181043903e8627211f04c217 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Fri, 25 Oct 2024 11:07:32 +0200 Subject: [PATCH 50/61] Updated changelog --- CHANGELOG.md | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 69d332e..259eb81 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -5,7 +5,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ## v1.0.0 - [date] -Initial release of nf-core/rangeland, created with the [nf-core](https://nf-co.re/) template. +First release of `nf-core/rangeland`. +This work is a continuation, and nf-core port, of the [original version of this pipeline](https://github.com/CRC-FONDA/FORCE2NXF-Rangeland). ### `Added` From 415edb40cca6cf1e8ad203b419b472b02c7dd7cc Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Sat, 26 Oct 2024 09:53:34 +0200 Subject: [PATCH 51/61] Fixed some linter warnings --- .nf-core.yml | 1 + conf/base.config | 1 - nextflow.config | 2 +- 3 files changed, 2 insertions(+), 2 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index 9981b0d..d29a63f 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -2,6 +2,7 @@ bump_version: null lint: files_exist: - conf/igenomes.config + - conf/igenomes_ignored.config nf_core_version: 3.0.2 org_path: null repository_type: pipeline diff --git a/conf/base.config b/conf/base.config index 7dde9f4..cf3d991 100644 --- a/conf/base.config +++ b/conf/base.config @@ -10,7 +10,6 @@ process { - // TODO nf-core: Check the defaults for all processes cpus = { 1 * task.attempt } memory = { 6.GB * task.attempt } time = { 4.h * task.attempt } diff --git a/nextflow.config b/nextflow.config index 808a38d..eac20e6 100644 --- a/nextflow.config +++ b/nextflow.config @@ -181,7 +181,7 @@ profiles { includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/nfcore_custom.config" : "/dev/null" // Load nf-core/rangeland custom profiles from different institutions. -// TODO nf-core: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs +// nf-core: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/rangeland.config" : "/dev/null" // Set default registry for Apptainer, Docker, Podman, Charliecloud and Singularity independent of -profile From 0585e5e36c97a010e18b5c127d7b8a19c9aec549 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Mon, 28 Oct 2024 20:02:07 +0100 Subject: [PATCH 52/61] Replaced for-loops with GNU parallel --- modules/local/force-higher_level/main.nf | 8 +++----- modules/local/force-mosaic/main.nf | 13 +++++++++---- modules/local/merge/main.nf | 20 ++++++++++++-------- 3 files changed, 24 insertions(+), 17 deletions(-) diff --git a/modules/local/force-higher_level/main.nf b/modules/local/force-higher_level/main.nf index f174453..c042cb1 100644 --- a/modules/local/force-higher_level/main.nf +++ b/modules/local/force-higher_level/main.nf @@ -25,14 +25,12 @@ process FORCE_HIGHER_LEVEL { mkdir prov sed -i "/^DIR_PROVENANCE /c\\DIR_PROVENANCE = prov/" \$PARAM - + # higher level processing force-higher-level \$PARAM - #Rename files: /trend// to _, otherwise we can not reextract the tile name later + # Rename files: /trend// to _, otherwise we can not reextract the tile name later results=`find trend -name '*.tif*'` - for path in \$results; do - mv \$path \${path%/*}_\${path##*/} - done; + parallel -j $task.cpus 'mv {} {//}_{/}' ::: \$results cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/local/force-mosaic/main.nf b/modules/local/force-mosaic/main.nf index 604df9b..a114805 100644 --- a/modules/local/force-mosaic/main.nf +++ b/modules/local/force-mosaic/main.nf @@ -17,13 +17,18 @@ process FORCE_MOSAIC{ script: """ - #Move files from trend/_ to trend// - results=`find trend/*.tif*` - for path in \$results; do + move_file() { + path=\$1 mkdir -p \${path%_$product*} mv \$path \${path%_$product*}/${product}.\${path#*.} - done; + } + export -f move_file + + # Move files from trend/_ to trend// + results=`find trend/*.tif*` + parallel -j $task.cpus move_file ::: \$results + # start mosaic computation force-mosaic trend/ cat <<-END_VERSIONS > versions.yml diff --git a/modules/local/merge/main.nf b/modules/local/merge/main.nf index 812a9de..f849269 100644 --- a/modules/local/merge/main.nf +++ b/modules/local/merge/main.nf @@ -19,19 +19,19 @@ process MERGE { script: """ + # get files to merge files=`find -L input/ -type f -printf "%f\\n" | sort | uniq` numberFiles=`echo \$files | wc -w` - currentFile=0 - for file in \$files - do - currentFile=\$((currentFile+1)) - echo "Merging \$file (\$currentFile of \$numberFiles)" + # merge function + merge() { + file=\$1 + echo "Merging \$file (\$2 of \$numberFiles)" onefile=`ls -- */*/\${file} | head -1` - - #merge together matchingFiles=`ls -- */*/\${file}` + + # merge script execution depending on file type if [ "$data_type" = "boa" ]; then merge_boa.r \$file \${matchingFiles} elif [ "$data_type" = "qai" ]; then @@ -40,8 +40,12 @@ process MERGE { #apply meta force-mdcp \$onefile \$file + } + export -f merge + export numberFiles - done; + # start merging in parallel + parallel -j $task.cpus merge {} {#} ::: \$files cat <<-END_VERSIONS > versions.yml "${task.process}": From bd82d81690adb50c996b6f072261fc1f3225b57d Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Sat, 9 Nov 2024 11:04:22 +0100 Subject: [PATCH 53/61] Migrated check result modules to nf-test --- .gitignore | 2 + conf/test.config | 14 +---- conf/test_full.config | 14 ++--- nf-test.config | 8 +++ tests/check_test_results.nf.test | 54 +++++++++++++++++++ tests/nextflow.config | 5 ++ tests_large/check_test_full_results.nf.test | 57 +++++++++++++++++++++ workflows/rangeland.nf | 34 +----------- 8 files changed, 132 insertions(+), 56 deletions(-) create mode 100644 nf-test.config create mode 100644 tests/check_test_results.nf.test create mode 100644 tests/nextflow.config create mode 100644 tests_large/check_test_full_results.nf.test diff --git a/.gitignore b/.gitignore index a42ce01..8b0033e 100644 --- a/.gitignore +++ b/.gitignore @@ -7,3 +7,5 @@ testing/ testing* *.pyc null/ +.nf-test.log +.nf-test/* diff --git a/conf/test.config b/conf/test.config index 39e2cf6..c874060 100644 --- a/conf/test.config +++ b/conf/test.config @@ -37,23 +37,11 @@ params { sensors_level2 = 'LND04 LND05' - // Reference data - woody_change_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/woody_cover_chg_ref.tif' - woody_yoc_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/woody_cover_yoc_ref.tif' - - herbaceous_change_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/herbaceous_cover_chg_ref.tif' - herbaceous_yoc_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/herbaceous_cover_yoc_ref.tif' - - peak_change_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/peak_chg_ref.tif' - peak_yoc_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/peak_yoc_ref.tif' - // Other parameters group_size = 10 - // enable mosaic for result checking + // enable mosaic visualization mosaic_visualization = true - - validationSchemaIgnoreParams = "peak_yoc_ref,peak_change_ref,herbaceous_yoc_ref,herbaceous_change_ref,woody_yoc_ref,woody_change_ref" } process { diff --git a/conf/test_full.config b/conf/test_full.config index d6851b2..b2df818 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -30,24 +30,16 @@ params { sensors_level2 = 'LND04 LND05' - // enable time series stack output + // Enable time series stack output return_tss = true - // enable mosaic for result checking (required for result checking) + // Enable mosaic visualization mosaic_visualization = true - - // reference data - reference = "s3://ngi-igenomes/test-data/rangeland/reference.tar" - - validationSchemaIgnoreParams = "reference" - } process { + // This is required because of the internal structure of the input tarballs withName: "UNTAR_*" { ext.args2 = "--strip-components=1" } - withName: "UNTAR_REF"{ - ext.args2 = "--strip-components=0" - } } diff --git a/nf-test.config b/nf-test.config new file mode 100644 index 0000000..a2ad2ce --- /dev/null +++ b/nf-test.config @@ -0,0 +1,8 @@ +config { + + testsDir "tests" + workDir ".nf-test" + configFile "tests/nextflow.config" + profile "docker,test" + +} diff --git a/tests/check_test_results.nf.test b/tests/check_test_results.nf.test new file mode 100644 index 0000000..27c41a6 --- /dev/null +++ b/tests/check_test_results.nf.test @@ -0,0 +1,54 @@ +// This is a pipeline level-test, not a module test, even though the main script is a module. +// The entire workflow is executed in the setup scope using the same config as the test profile. +// Afterwards, a single module is executed in the when scope to ensure that the pipeline generated correct results. + +nextflow_process { + + name "Check the correctness of the pipeline results using the test profile" + script "../modules/local/check_results/main.nf" + process "CHECK_RESULTS" + + test("Checking pipeline results for: -profile test") { + + setup { + run("NFCORE_RANGELAND") { + script "../main.nf" + workflow {} + } + + } + + when { + params { + // Reference data + woody_change_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/woody_cover_chg_ref.tif' + woody_yoc_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/woody_cover_yoc_ref.tif' + + herbaceous_change_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/herbaceous_cover_chg_ref.tif' + herbaceous_yoc_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/herbaceous_cover_yoc_ref.tif' + + peak_change_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/peak_chg_ref.tif' + peak_yoc_ref = 'https://github.com/nf-core/test-datasets/raw/rangeland/reference/peak_yoc_ref.tif' + } + + process { + """ + // inputs for CHECK_RESULTS + input[0] = NFCORE_RANGELAND.out.mosaic.map{ it[1] }.flatten().buffer( size: Integer.MAX_VALUE, remainder: true ) + input[1] = file( params.woody_change_ref ) + input[2] = file( params.woody_yoc_ref ) + input[3] = file( params.herbaceous_change_ref ) + input[4] = file( params.herbaceous_yoc_ref ) + input[5] = file( params.peak_change_ref ) + input[6] = file( params.peak_yoc_ref ) + """ + } + } + + then { + assert process.success + } + + } + +} diff --git a/tests/nextflow.config b/tests/nextflow.config new file mode 100644 index 0000000..c19b1ad --- /dev/null +++ b/tests/nextflow.config @@ -0,0 +1,5 @@ +/* +======================================================================================== + Nextflow config file for running tests +======================================================================================== +*/ diff --git a/tests_large/check_test_full_results.nf.test b/tests_large/check_test_full_results.nf.test new file mode 100644 index 0000000..5ccf3e0 --- /dev/null +++ b/tests_large/check_test_full_results.nf.test @@ -0,0 +1,57 @@ +// WARNING: This test requires approximately 400GB of free disk space. +// It is excluded from default test runs due to its high resource demands. +// Run with caution. + +// This is a pipeline level-test, not a module test, even though the main script is a module. +// The entire workflow is executed in the setup scope using the same config as the test_full profile. +// Next, still within the setup scope the reference data is pulled and extracted. +// Afterwards, a single module is executed in the when scope to ensure that the pipeline generated correct results. + +nextflow_process { + + name "Check the correctness of the pipeline results using the test_full profile" + script "../modules/local/check_results_full/main.nf" + process "CHECK_RESULTS_FULL" + config "../conf/test_full.config" + + test("Checking pipeline results for: -profile test_full") { + + setup { + run("NFCORE_RANGELAND") { + script "../main.nf" + workflow {} + } + + run("UNTAR", alias: "UNTAR_REF" ) { + script "../modules/nf-core/untar/main.nf" + process { + """ + input[0] = [[:], params.reference] + """ + } + } + + } + + when { + params { + // reference data + reference = "s3://ngi-igenomes/test-data/rangeland/reference.tar" + } + + process { + """ + // inputs for CHECK_RESULTS_FULL + input[0] = NFCORE_RANGELAND.out.mosaic.map{ it[1] }.flatten().buffer( size: Integer.MAX_VALUE, remainder: true ) + input[1] = UNTAR_REF.out.untar.map(it->it[1]) + """ + } + } + + then { + assert process.success + } + + } + +} diff --git a/workflows/rangeland.nf b/workflows/rangeland.nf index 879ce00..905b9f4 100644 --- a/workflows/rangeland.nf +++ b/workflows/rangeland.nf @@ -22,12 +22,6 @@ include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_rang include { PREPROCESSING } from '../subworkflows/local/preprocessing' include { HIGHER_LEVEL } from '../subworkflows/local/higher_level' -// -// MODULES -// - -include { CHECK_RESULTS } from '../modules/local/check_results/main' -include { CHECK_RESULTS_FULL } from '../modules/local/check_results_full/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT NF-CORE MODULES/SUBWORKFLOWS @@ -135,6 +129,8 @@ workflow RANGELAND { wvdb = wvdb.mix(ch_untared_wvdb, ch_wvdb_types.dirs).first() + ch_versions = ch_versions.mix(tar_versions.first()) + // // SUBWORKFLOW: Preprocess satellite imagery // @@ -169,32 +165,6 @@ workflow RANGELAND { grouped_trend_data = HIGHER_LEVEL.out.mosaic.map{ it[1] }.flatten().buffer( size: Integer.MAX_VALUE, remainder: true ) - // - // MODULE: Check results - // - if (params.config_profile_name == 'Test profile') { - woody_change_ref = file( params.woody_change_ref ) - woody_yoc_ref = file( params.woody_yoc_ref ) - herbaceous_change_ref = file( params.herbaceous_change_ref ) - herbaceous_yoc_ref = file( params.herbaceous_yoc_ref ) - peak_change_ref = file( params.peak_change_ref ) - peak_yoc_ref = file( params.peak_yoc_ref ) - - CHECK_RESULTS(grouped_trend_data, woody_change_ref, woody_yoc_ref, herbaceous_change_ref, herbaceous_yoc_ref, peak_change_ref, peak_yoc_ref) - ch_versions = ch_versions.mix(CHECK_RESULTS.out.versions) - } - - if (params.config_profile_name == 'Full test profile') { - UNTAR_REF([[:], params.reference]) - ref_path = UNTAR_REF.out.untar.map(it -> it[1]) - tar_versions.mix(UNTAR_REF.out.versions) - - CHECK_RESULTS_FULL(grouped_trend_data, ref_path) - ch_versions = ch_versions.mix(CHECK_RESULTS_FULL.out.versions) - } - - ch_versions = ch_versions.mix(tar_versions.first()) - // // Collate and save software versions // From a8da0e345314bf4385a351a58d1268175671baba Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Mon, 11 Nov 2024 11:53:54 +0100 Subject: [PATCH 54/61] Added parameter to disable all output publishing --- conf/modules.config | 51 +++++++++++++++++++++++++++----------------- docs/output.md | 2 ++ docs/usage.md | 13 +++++++++++ nextflow.config | 7 +++--- nextflow_schema.json | 7 ++++++ 5 files changed, 58 insertions(+), 22 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 911dce9..d310232 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -13,16 +13,18 @@ process { publishDir = [ - path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + enabled: params.publish_dir_enabled ] withName: "FORCE_GENERATE_ANALYSIS_MASK" { publishDir = [ path: { "${params.outdir}/preparation/" }, mode: params.publish_dir_mode, - pattern: '**.tif' + pattern: '**.tif', + enabled: params.publish_dir_enabled ] } @@ -30,7 +32,8 @@ process { publishDir = [ path: { "${params.outdir}/preparation/" }, pattern: '*.txt', - mode: params.publish_dir_mode + mode: params.publish_dir_mode, + enabled: params.publish_dir_enabled ] } @@ -39,13 +42,15 @@ process { [ path: { "${params.outdir}/preprocess/${task.tag}/logs" }, mode: params.publish_dir_mode, - pattern: '*.log' + pattern: '*.log', + enabled: params.publish_dir_enabled ], [ path: { "${params.outdir}/preprocess/${task.tag}" }, mode: params.publish_dir_mode, pattern: 'level2_ard/**/*', - saveAs: { params.save_ard ? it : null } + saveAs: { params.save_ard ? it : null }, + enabled: params.publish_dir_enabled ] ] } @@ -55,7 +60,8 @@ process { publishDir = [ path: { "${params.outdir}/higher-level/${task.tag}/param_files" }, mode: params.publish_dir_mode, - pattern: '*.prm' + pattern: '*.prm', + enabled: params.publish_dir_enabled ] } @@ -64,7 +70,8 @@ process { path: { "${params.outdir}/higher-level/${task.tag}" }, mode: params.publish_dir_mode, pattern: 'trend/*.tif', - saveAs: { params.save_ard ? "trend_files/${it.tokenize('/')[-1]}" : null } + saveAs: { params.save_ard ? "trend_files/${it.tokenize('/')[-1]}" : null }, + enabled: params.publish_dir_enabled ] } @@ -75,12 +82,14 @@ process { path: { "${params.outdir}/trend/pyramid/" }, saveAs: { "${it.substring(12,it.indexOf("."))}/trend/${it.substring(0,11)}/$it" }, pattern: '*.tif*', - mode: params.publish_dir_mode + mode: params.publish_dir_mode, + enabled: params.publish_dir_enabled ], [ path: { "${params.outdir}/trend/pyramid/" }, pattern: 'versions.yml', - mode: params.publish_dir_mode + mode: params.publish_dir_mode, + enabled: params.publish_dir_enabled ] ] } @@ -89,7 +98,8 @@ process { publishDir = [ path: { "${params.outdir}/trend/mosaic/" }, mode: params.publish_dir_mode, - saveAs: {"${params.outdir}/trend/mosaic/${task.tag}/${it.replaceAll("trend/","")}"} + saveAs: {"${params.outdir}/trend/mosaic/${task.tag}/${it.replaceAll("trend/","")}"}, + enabled: params.publish_dir_enabled ] } @@ -109,24 +119,27 @@ process { publishDir = [ path: { "${params.outdir}/preprocess/${task.tag}/param_files" }, mode: params.publish_dir_mode, - pattern: '*.prm' + pattern: '*.prm', + enabled: params.publish_dir_enabled ] } withName: 'MULTIQC' { ext.args = params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' publishDir = [ - path: { "${params.outdir}/multiqc" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + path: { "${params.outdir}/multiqc" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + enabled: params.publish_dir_enabled ] } withName: "UNTAR_*" { publishDir = [ - path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }, - mode: 'symlink', - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }, + mode: 'symlink', + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + enabled: params.publish_dir_enabled ] } diff --git a/docs/output.md b/docs/output.md index cc7f24d..8769c5a 100644 --- a/docs/output.md +++ b/docs/output.md @@ -7,6 +7,8 @@ This document describes the output produced by the pipeline. The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. +Note that running this pipeline with `--publish_dir_enabled false` will prevent any module from publishing its output. See [Usage](usage.md#module-output-publishing) for details + ## Pipeline overview The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: diff --git a/docs/usage.md b/docs/usage.md index 623bf5d..3fb0723 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -348,6 +348,19 @@ Publishing of intermediate data can be enabled using: --save_tsa = '[boolean]' ``` +### Module output publishing + +Certain users may not be interested in the pipeline's outputs. +To disable all modules from publishing their outputs, the `--publish_dir_enabled` can be set to `false`. +Note that this affects _all modules_. +By default all modules publish their outputs according to the other parameters. + +Publishing of all module's outputs can be enabled or disabled using: + +```bash +--publish_dir_enabled = '[boolean]' +``` + ## Running the pipeline The typical command for running the pipeline is as follows: diff --git a/nextflow.config b/nextflow.config index eac20e6..8edbb7c 100644 --- a/nextflow.config +++ b/nextflow.config @@ -24,9 +24,10 @@ params { end_date = "2006-12-31" // Workflow configuration - group_size = 100 - save_ard = false - save_tsa = false + group_size = 100 + save_ard = false + save_tsa = false + publish_dir_enabled = true // Higher Level processing configuration indexes = "NDVI BLUE GREEN RED NIR SWIR1 SWIR2" diff --git a/nextflow_schema.json b/nextflow_schema.json index 5829b02..173a047 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -198,6 +198,13 @@ "description": "Batch size of tiles considered for merging.", "help_text": "Controls trade-off between parallelism and I/O load.\nHigher group_size -> More tiles merged in one process, less I/O load.\nLower group_size -> Less tiles merged in one process, more I/O load. ", "fa_icon": "fas fa-layer-group" + }, + "publish_dir_enabled": { + "type": "boolean", + "default": true, + "description": "Publish pipeline outputs.", + "help_text": "Set to `false` to prevent *all* modules from publishing their results.", + "fa_icon": "fas fa-database" } }, "help_text": "You can savely use the defaults.", From e67107df6ae7f326268d844e5d0e8e912b14f189 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Wed, 13 Nov 2024 11:56:28 +0100 Subject: [PATCH 55/61] Fixed log output for preprocessing --- conf/modules.config | 2 +- modules/local/force-preprocess/main.nf | 4 ++-- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index d310232..9eece2d 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -42,7 +42,7 @@ process { [ path: { "${params.outdir}/preprocess/${task.tag}/logs" }, mode: params.publish_dir_mode, - pattern: '*.log', + pattern: '**.log', enabled: params.publish_dir_enabled ], [ diff --git a/modules/local/force-preprocess/main.nf b/modules/local/force-preprocess/main.nf index d101429..e694b6c 100644 --- a/modules/local/force-preprocess/main.nf +++ b/modules/local/force-preprocess/main.nf @@ -11,7 +11,7 @@ process FORCE_PREPROCESS { output: path "**/*BOA.tif", optional:true, emit: boa_tiles path "**/*QAI.tif", optional:true, emit: qai_tiles - path "*.log" , emit: log + path "**.log" , emit: log path "versions.yml" , emit: versions when: @@ -36,7 +36,7 @@ process FORCE_PREPROCESS { FILEPATH=$data BASE=\$(basename $data) - force-l2ps \$FILEPATH \$PARAM > level2_log\$BASE.log + force-l2ps \$FILEPATH \$PARAM > level2_log/\$BASE.log cat <<-END_VERSIONS > versions.yml "${task.process}": From 88ea70f3d84041cf7dfa3c973db78136385e538f Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Sun, 24 Nov 2024 15:47:09 +0100 Subject: [PATCH 56/61] Added pipeline-level nf-test tests --- nf-test.config | 3 + tests/default.nf.test | 26 + tests/default.nf.test.snap | 1274 +++++++++++++++++++ tests/tweaked_params.nf.test | 32 + tests/tweaked_params.nf.test.snap | 947 ++++++++++++++ tests_large/check_test_full_results.nf.test | 4 +- 6 files changed, 2284 insertions(+), 2 deletions(-) create mode 100644 tests/default.nf.test create mode 100644 tests/default.nf.test.snap create mode 100644 tests/tweaked_params.nf.test create mode 100644 tests/tweaked_params.nf.test.snap diff --git a/nf-test.config b/nf-test.config index a2ad2ce..843ed05 100644 --- a/nf-test.config +++ b/nf-test.config @@ -5,4 +5,7 @@ config { configFile "tests/nextflow.config" profile "docker,test" + plugins { + load "nft-utils@0.0.3" + } } diff --git a/tests/default.nf.test b/tests/default.nf.test new file mode 100644 index 0000000..31f3c3e --- /dev/null +++ b/tests/default.nf.test @@ -0,0 +1,26 @@ +nextflow_pipeline { + + name "Test pipeline with default test profile settings " + script "../main.nf" + + test("Params: test profile default") { + + when { + params { + outdir = "$outputDir" + } + } + + then { + def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) + assert workflow.success + assert snapshot( + workflow.trace.succeeded().size(), + stable_name, + ).match() + + } + + } + +} diff --git a/tests/default.nf.test.snap b/tests/default.nf.test.snap new file mode 100644 index 0000000..d652eae --- /dev/null +++ b/tests/default.nf.test.snap @@ -0,0 +1,1274 @@ +{ + "Params: test profile default": { + "content": [ + 280, + [ + "higher-level", + "higher-level/X0109_Y0102", + "higher-level/X0109_Y0102/param_files", + "higher-level/X0109_Y0102/param_files/trend_X0109_Y0102.prm", + "multiqc", + "multiqc/multiqc_data", + "multiqc/multiqc_data/multiqc.log", + "multiqc/multiqc_data/multiqc_citations.txt", + "multiqc/multiqc_data/multiqc_data.json", + "multiqc/multiqc_data/multiqc_software_versions.txt", + "multiqc/multiqc_data/multiqc_sources.txt", + "multiqc/multiqc_report.html", + "pipeline_info", + "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", + "preparation", + "preparation/mask", + "preparation/mask/X0103_Y0101", + "preparation/mask/X0103_Y0101/aoi.tif", + "preparation/mask/X0103_Y0102", + "preparation/mask/X0103_Y0102/aoi.tif", + "preparation/mask/X0103_Y0103", + "preparation/mask/X0103_Y0103/aoi.tif", + "preparation/mask/X0103_Y0104", + "preparation/mask/X0103_Y0104/aoi.tif", + "preparation/mask/X0103_Y0105", + "preparation/mask/X0103_Y0105/aoi.tif", + "preparation/mask/X0104_Y0101", + "preparation/mask/X0104_Y0101/aoi.tif", + "preparation/mask/X0104_Y0102", + "preparation/mask/X0104_Y0102/aoi.tif", + "preparation/mask/X0104_Y0103", + "preparation/mask/X0104_Y0103/aoi.tif", + "preparation/mask/X0104_Y0104", + "preparation/mask/X0104_Y0104/aoi.tif", + "preparation/mask/X0104_Y0105", + "preparation/mask/X0104_Y0105/aoi.tif", + "preparation/mask/X0105_Y0101", + "preparation/mask/X0105_Y0101/aoi.tif", + "preparation/mask/X0105_Y0102", + 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"24.10.0" + }, + "timestamp": "2024-11-24T15:08:30.683117395" + } +} \ No newline at end of file diff --git a/tests/tweaked_params.nf.test b/tests/tweaked_params.nf.test new file mode 100644 index 0000000..f2a170c --- /dev/null +++ b/tests/tweaked_params.nf.test @@ -0,0 +1,32 @@ +nextflow_pipeline { + + name "Test pipeline with non-default parameters" + script "../main.nf" + + test("Params: --save_ard true --save_tsa true --return_tss true --sensors_level2 'LND05' --indexes 'RED NIR SWIR2' --end_date '1988-12-31'") { + + when { + params { + outdir = "$outputDir" + save_ard = true + save_tsa = true + return_tss = true + sensors_level2 = "LND05" + indexes = "RED NIR SWIR2" + end_date = "1988-12-31" + } + } + + then { + def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) + assert workflow.success + assert snapshot( + workflow.trace.succeeded().size(), + stable_name, + ).match() + + } + + } + +} diff --git 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"trend/pyramid/1987-1988_001-365_HL_TSA_LNDLG_SW2_VSA-TRO/trend/X0109_Y0102", + "trend/pyramid/1987-1988_001-365_HL_TSA_LNDLG_SW2_VSA-TRO/trend/X0109_Y0102/X0109_Y0102_1987-1988_001-365_HL_TSA_LNDLG_SW2_VSA-TRO.tif.ovr", + "trend/pyramid/versions.yml", + "untar", + "untar/Landsat_data", + "untar/dem", + "untar/wvdb" + ] + ], + "meta": { + "nf-test": "0.9.1", + "nextflow": "24.10.0" + }, + "timestamp": "2024-11-24T15:43:30.671676472" + } +} \ No newline at end of file diff --git a/tests_large/check_test_full_results.nf.test b/tests_large/check_test_full_results.nf.test index 5ccf3e0..4a2affe 100644 --- a/tests_large/check_test_full_results.nf.test +++ b/tests_large/check_test_full_results.nf.test @@ -1,4 +1,4 @@ -// WARNING: This test requires approximately 400GB of free disk space. +// WARNING: This test requires approximately 400GB of free space. // It is excluded from default test runs due to its high resource demands. // Run with caution. @@ -14,7 +14,7 @@ nextflow_process { process "CHECK_RESULTS_FULL" config "../conf/test_full.config" - test("Checking pipeline results for: -profile test_full") { + test("Checking pipeline results for: -profile test_full | requires 400GB free space") { setup { run("NFCORE_RANGELAND") { From 72996b1c62380a33a1759b666b546ebebea4f55c Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Tue, 26 Nov 2024 14:13:41 +0100 Subject: [PATCH 57/61] Aligned run command in usage docs with other pipelines --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index 3fb0723..e70cee4 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -366,7 +366,7 @@ Publishing of all module's outputs can be enabled or disabled using: The typical command for running the pipeline is as follows: ```bash -nextflow run nf-core/rangeland --input --dem --wvdb --data_cube --aoi --endmember --outdir -profile docker +nextflow run nf-core/rangeland -profile docker --input --dem --wvdb --data_cube --aoi --endmember --outdir ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. From ba1d17f502312698f2ce0959d6eda5049f095db7 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Tue, 26 Nov 2024 14:13:59 +0100 Subject: [PATCH 58/61] Updated nf-core workflows and modules --- modules.json | 8 ++-- modules/nf-core/untar/meta.yml | 43 ++++++++++--------- .../nf-core/utils_nextflow_pipeline/main.nf | 2 + .../tests/main.workflow.nf.test | 10 +++-- .../nf-core/utils_nfcore_pipeline/main.nf | 7 ++- .../utils_nfschema_plugin/tests/main.nf.test | 4 +- 6 files changed, 42 insertions(+), 32 deletions(-) diff --git a/modules.json b/modules.json index b2fa7c5..5281958 100644 --- a/modules.json +++ b/modules.json @@ -12,7 +12,7 @@ }, "untar": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] } } @@ -21,17 +21,17 @@ "nf-core": { "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "3aa0aec1d52d492fe241919f0c6100ebf0074082", + "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "1b6b9a3338d011367137808b49b923515080e3ba", + "git_sha": "1b89f75f1aa2021ec3360d0deccd0f6e97240551", "installed_by": ["subworkflows"] }, "utils_nfschema_plugin": { "branch": "master", - "git_sha": "bbd5a41f4535a8defafe6080e00ea74c45f4f96c", + "git_sha": "2fd2cd6d0e7b273747f32e465fdc6bcc3ae0814e", "installed_by": ["subworkflows"] } } diff --git a/modules/nf-core/untar/meta.yml b/modules/nf-core/untar/meta.yml index a9a2110..290346b 100644 --- a/modules/nf-core/untar/meta.yml +++ b/modules/nf-core/untar/meta.yml @@ -10,30 +10,33 @@ tools: Extract tar.gz files. documentation: https://www.gnu.org/software/tar/manual/ licence: ["GPL-3.0-or-later"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - archive: - type: file - description: File to be untar - pattern: "*.{tar}.{gz}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - archive: + type: file + description: File to be untar + pattern: "*.{tar}.{gz}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - untar: - type: directory - description: Directory containing contents of archive - pattern: "*/" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - $prefix: + type: directory + description: Directory containing contents of archive + pattern: "*/" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@joseespinosa" - "@drpatelh" diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf index 0fcbf7b..d6e593e 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -92,10 +92,12 @@ def checkCondaChannels() { channels = config.channels } catch (NullPointerException e) { + log.debug(e) log.warn("Could not verify conda channel configuration.") return null } catch (IOException e) { + log.debug(e) log.warn("Could not verify conda channel configuration.") return null } diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test index ca964ce..02dbf09 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -52,10 +52,12 @@ nextflow_workflow { } then { - assertAll( - { assert workflow.success }, - { assert workflow.stdout.contains("nextflow_workflow v9.9.9") } - ) + expect { + with(workflow) { + assert success + assert "nextflow_workflow v9.9.9" in stdout + } + } } } diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf index 5cb7baf..4cd3362 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -261,7 +261,8 @@ def attachMultiqcReport(multiqc_report) { } } } - catch (Exception all) { + catch (Exception msg) { + log.debug(msg) if (multiqc_report) { log.warn("[${workflow.manifest.name}] Could not attach MultiQC report to summary email") } @@ -358,7 +359,9 @@ new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML') ['sendmail', '-t'].execute() << sendmail_html log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Sent summary e-mail to ${email_address} (sendmail)-") } - catch (Exception all) { + catch (Exception msg) { + log.debug(msg) + log.debug("Trying with mail instead of sendmail") // Catch failures and try with plaintext def mail_cmd = ['mail', '-s', subject, '--content-type=text/html', email_address] mail_cmd.execute() << email_html diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test index 842dc43..8fb3016 100644 --- a/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test +++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test @@ -42,7 +42,7 @@ nextflow_workflow { params { test_data = '' - outdir = 1 + outdir = null } workflow { @@ -94,7 +94,7 @@ nextflow_workflow { params { test_data = '' - outdir = 1 + outdir = null } workflow { From d8be4ef053991c90766c15ac3b88ed440d86dec9 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Tue, 26 Nov 2024 15:32:45 +0100 Subject: [PATCH 59/61] Fix nf-test entry in .gitignore MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- .gitignore | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/.gitignore b/.gitignore index 8b0033e..23b0c7d 100644 --- a/.gitignore +++ b/.gitignore @@ -7,5 +7,4 @@ testing/ testing* *.pyc null/ -.nf-test.log -.nf-test/* +.nf-test* From 3d642274cab5550d092a5327da527e6a495076db Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Tue, 26 Nov 2024 15:43:09 +0100 Subject: [PATCH 60/61] Fixed wrong pipeline version in usage.md MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index e70cee4..4e45c11 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -426,7 +426,7 @@ It is a good idea to specify a pipeline version when running the pipeline on you This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. -First, go to the [nf-core/rangeland releases page](https://github.com/nf-core/rangeland/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). +First, go to the [nf-core/rangeland releases page](https://github.com/nf-core/rangeland/releases) and find the latest pipeline version - numeric only (eg. `1.0.0`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports. From a651b5562bc92002482b71546994b2e5d27daf53 Mon Sep 17 00:00:00 2001 From: Felix Kummer Date: Tue, 26 Nov 2024 15:57:12 +0100 Subject: [PATCH 61/61] Added concrete boolean values for parameters in usage.md --- docs/usage.md | 14 +++++++------- 1 file changed, 7 insertions(+), 7 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 4e45c11..7f73f5f 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -316,7 +316,7 @@ The option is disabled by default to reduce the output size. The time series stack output can be enabled using: ```bash ---return_tss '[boolean]' +--return_tss true ``` ### Visualization @@ -331,8 +331,8 @@ Note that the mosaic visualization is required to be enabled when using the `tes The visualizations can be enabled using: ```bash ---mosaic_visualization = '[boolean]' ---pyramid_visualization = '[boolean]' +--mosaic_visualization = true +--pyramid_visualization = true ``` ### Intermediate data publishing @@ -344,8 +344,8 @@ The files for preprocessing and higher level processing can, therefore, be publi Publishing of intermediate data can be enabled using: ```bash ---save_ard = '[boolean]' ---save_tsa = '[boolean]' +--save_ard = true +--save_tsa = true ``` ### Module output publishing @@ -355,10 +355,10 @@ To disable all modules from publishing their outputs, the `--publish_dir_enabled Note that this affects _all modules_. By default all modules publish their outputs according to the other parameters. -Publishing of all module's outputs can be enabled or disabled using: +Publishing of all module's outputs can be disabled using: ```bash ---publish_dir_enabled = '[boolean]' +--publish_dir_enabled = false ``` ## Running the pipeline
    Process Name \\", - " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
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