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params.config
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params.config
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params {
//============================Required parameters=======================================================================
mode = 'test' //Set mode to 'prod' to run on new dataset otherwise brioche will run on the test data
notifications = true //Option to (dis|en)able email notifications, set to false to disable
emailaddress = "" //Replace with your e-mail address to receive notifications
resultsdir = "" //Path where brioche results are saved, default is the launch-directory/brioche-results
genomefasta = "${projectDir}/Data/CDCFrontier_GA_v10_Chrom_3_7.fa" //Replace with absolute path to the fasta file for your assembly
restrict2chrom = "" //Restrict search to this chromosome
chromstoexclude = "" //List of chromosomes to exclude separated by a comma - e.g "chr1,chr2,chrun"
genomename = 'CDCFrontier_GA_v10' //Name of genome that is used in naming output files
probename = 'AVRGRDC_Pulses_v1' //Name of Chip/Marker set used in the naming of the output file
istarget3primeend = 'TRUE' //Is the target in the probe at the 3' prime end, 'TRUE' or 'FALSE'
targetdesign = "${projectDir}/Data/AVRGRDC_Pulses_v1_20006795X370754_A2_Chickpea-target-new-format.tsv" //csv or tsv table with ID,ProbeSequence,Target bp position, Target
markercharacter = 'D' //Character used to replace target marker in probe sequence
buildblastdbonly = false //Set to true, if all you want to do is build a blast database
//============================Options for BLAST=======================================================================
evalue = 0.05 //The BLAST E-value is the number of expected hits of similar quality (score) that could be found just by chance.
dust = 'no' //Filtering option to filter query sequence with DUST (Format: 'yes', 'level window linker', or 'no' to disable)
otherblastoptions = '' //Other parameters that need to be passed to BLAST, should be of the format "-param1 val1 -param2 val2"
//================================XT data with target SNP at end of probe===============================================
minlength = 40 //minimum length of HSP to be considered fully hybridized
extendablebps = 3 //number of matching base pairs from the 3 prime end for a probe to be considered as extendable
maxgaps = 0 // New option to implement
//================================Filtering options====================================================================
coverage = 70 //Option to filter any hits with coverage less than the provided percentage
pident = 90 //Option to filter any hits with pident less than the provided percentage
maximumhits = 10 //Filter probes with hits more than maximumhits - change in logic
keepduplicates = 'FALSE' //Whether to retain or filter markers with multiple hits, maximumhits
//================================slurm options====================================================================
hpcaccount = 'default' //account to be used on basc, change this to the account associated with the project etc
shortqueue = 'shortrun' //queueing option for jobs with short run time
longqueue = 'batch' //queueing option for jobs with long run times
queuesize = 1000 //Number of jobs submitted to slurm queue at any given time
}