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Error with filter-by-length step for some samples. #21

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ebujaki opened this issue Sep 25, 2024 · 0 comments
Open

Error with filter-by-length step for some samples. #21

ebujaki opened this issue Sep 25, 2024 · 0 comments

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@ebujaki
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ebujaki commented Sep 25, 2024

Hi,
I ran piranhaGUI with piranha 1.3.1 on samples with a lot of reads and it stopped with an error.
It appeared to have issues with completing to output files from the read length filtering step for some samples.
This version of MinKNOW creates a new readfile every 10 minutes for all detected barcodes, so file numbers are high for a long run and files sizes vary. As it seemed working fine with some barcodes I rerun with a much reduced number of fastqpass files for each and the analysis completed without any issues. Is there some kind of readnumber/dataamount/filesizeforfilteredoutput limit? Please see images for more info. Thanks.
IMG-20240925-WA0001
IMG-20240925-WA0003
IMG-20240925-WA0005

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