diff --git a/primerschemes/artic-marv/1200/v1.0.0/README.md b/primerschemes/artic-marv/1200/v1.0.0/README.md new file mode 100644 index 0000000..8009a14 --- /dev/null +++ b/primerschemes/artic-marv/1200/v1.0.0/README.md @@ -0,0 +1,61 @@ +# artic-marv 1200bp v1.0.0 + +[primalscheme labs](https://labs.primalscheme.com/detail/artic-marv/1200/v1.0.0) + +## Description + +Genomes for MARV and RAVV were downloaded from Genbank, aligned using MAFFT and alignment was used as the input for primalscheme3. + +## Overviews + +![plot.png](work/plot.png) + +## Details + +```json +{ + "ampliconsize": 1200, + "schemeversion": "v1.0.0", + "schemename": "artic-marv", + "primer_bed_md5": "481f2b61310fe3af58e6772e292822d7", + "reference_fasta_md5": "99fef270892d906f609712ec8d613381", + "status": "draft", + "citations": [], + "authors": [ + "ARTIC network", + "Quick Lab" + ], + "algorithmversion": "primalscheme3", + "species": [ + 3052505 + ], + "license": "CC BY-SA 4.0", + "primerclass": "primerschemes", + "infoschema": "v2.0.0", + "articbedversion": "v3.0", + "collections": [ + "ARTIC", + "WHOLE-GENOME", + "QUICK-LAB" + ], + "links": { + "protocols": [], + "validation": [], + "homepage": [], + "vendors": [], + "misc": [] + }, + "refselect": null, + "description": "Genomes for MARV and RAVV were downloaded from Genbank, aligned using MAFFT and alignment was used as the input for primalscheme3.", + "derivedfrom": null, + "contactinfo": null +} +``` + + + +------------------------------------------------------------------------ + +This work is licensed under a [Creative Commons Attribution-ShareAlike 4.0 International License](http://creativecommons.org/licenses/by-sa/4.0/) + +![](https://i.creativecommons.org/l/by-sa/4.0/88x31.png) \ No newline at end of file diff --git a/primerschemes/artic-marv/1200/v1.0.0/info.json b/primerschemes/artic-marv/1200/v1.0.0/info.json new file mode 100644 index 0000000..47fa6ec --- /dev/null +++ b/primerschemes/artic-marv/1200/v1.0.0/info.json @@ -0,0 +1,37 @@ +{ + "ampliconsize": 1200, + "schemeversion": "v1.0.0", + "schemename": "artic-marv", + "primer_bed_md5": "481f2b61310fe3af58e6772e292822d7", + "reference_fasta_md5": "99fef270892d906f609712ec8d613381", + "status": "draft", + "citations": [], + "authors": [ + "ARTIC network", + "Quick Lab" + ], + "algorithmversion": "primalscheme3", + "species": [ + 3052505 + ], + "license": "CC BY-SA 4.0", + "primerclass": "primerschemes", + "infoschema": "v2.0.0", + "articbedversion": "v3.0", + "collections": [ + "ARTIC", + "WHOLE-GENOME", + "QUICK-LAB" + ], + "links": { + "protocols": [], + "validation": [], + "homepage": [], + "vendors": [], + "misc": [] + }, + "refselect": null, + "description": "Genomes for MARV and RAVV were downloaded from Genbank, aligned using MAFFT and alignment was used as the input for primalscheme3.", + "derivedfrom": null, + "contactinfo": null +} \ No newline at end of file diff --git a/primerschemes/artic-marv/1200/v1.0.0/primer.bed b/primerschemes/artic-marv/1200/v1.0.0/primer.bed new file mode 100644 index 0000000..cf4873f --- /dev/null +++ b/primerschemes/artic-marv/1200/v1.0.0/primer.bed @@ -0,0 +1,128 @@ +#artic-bed-version v3.0 +NC_001608.3 101 133 2ee06dc7_1_LEFT_1 1 + AGATGGATTTACATAGTTTGCTAGAATTAGGT +NC_001608.3 101 133 2ee06dc7_1_LEFT_2 1 + AGATGGATTTACATAGTTTGTTAGAATTGGGT +NC_001608.3 103 133 2ee06dc7_1_LEFT_3 1 + ATGGATTTACATAGTTTGTTAGAGTTGGGT +NC_001608.3 105 133 2ee06dc7_1_LEFT_4 1 + GGATTTACATAGCTTGTTAGAGTTGGGT +NC_001608.3 104 133 2ee06dc7_1_LEFT_5 1 + TGGACTTACATAGTTTGTTAGAGTTGGGT +NC_001608.3 1481 1505 2ee06dc7_1_RIGHT_1 1 - CGTTCAGCAGTGCAAATGGATCAT +NC_001608.3 1481 1506 2ee06dc7_1_RIGHT_2 1 - TCATTCAGCAGTGCAAATGGATCAT +NC_001608.3 1481 1503 2ee06dc7_1_RIGHT_3 1 - TTCAGCAGCGCAAATGGATCAT +NC_001608.3 1290 1319 2ee06dc7_2_LEFT_1 2 + GGAAGATCAGGGATTTAAACAATCACAGA +NC_001608.3 1296 1319 2ee06dc7_2_LEFT_2 2 + TCAGGGGTTCAAACAATCGCAGA +NC_001608.3 2669 2697 2ee06dc7_2_RIGHT_1 2 - ACTCCAAACCAGTGTACTTGACATTCTA +NC_001608.3 2669 2697 2ee06dc7_2_RIGHT_2 2 - ACTCTGAACCAGTAGACTTGACATTCTA +NC_001608.3 2669 2698 2ee06dc7_2_RIGHT_3 2 - GACTCCAAACTTGTAGACTTGACATTCTA +NC_001608.3 2669 2690 2ee06dc7_2_RIGHT_4 2 - GCCAACAAACTCGGCGTTTCA +NC_001608.3 2669 2695 2ee06dc7_2_RIGHT_5 2 - TCCAAACCAGTGGACTTGACATTCTA +NC_001608.3 2417 2447 2ee06dc7_3_LEFT_1 1 + CCATAACGATACAACTATAAACCTCCGTAC +NC_001608.3 2417 2447 2ee06dc7_3_LEFT_2 1 + CCATGATGATACAACTATAAACCTCCGTAC +NC_001608.3 2416 2447 2ee06dc7_3_LEFT_3 1 + GCCATAATGATACAACTATAAACCTCCGTAC +NC_001608.3 2416 2447 2ee06dc7_3_LEFT_4 1 + GCTATAATGATACAACTACAAACCTCCGTAC +NC_001608.3 2416 2447 2ee06dc7_3_LEFT_5 1 + GCTATAATGATACAACTACGAACCTCCATAC +NC_001608.3 2415 2447 2ee06dc7_3_LEFT_6 1 + TGCTACGATAATACAACTACAAACCTCTTTAC +NC_001608.3 2415 2447 2ee06dc7_3_LEFT_7 1 + TGCTATAATGATACAACTACAAACCTCTGTAC +NC_001608.3 2415 2447 2ee06dc7_3_LEFT_8 1 + TGCTATAATGATACAACTACAAATCTCCGTAC +NC_001608.3 3778 3809 2ee06dc7_3_RIGHT_1 1 - AGTTTTTAACTTTTATTACTGGAGGAACTGC +NC_001608.3 3778 3803 2ee06dc7_3_RIGHT_2 1 - TGACTCTTATCACTGGAGGAACCAC +NC_001608.3 3778 3807 2ee06dc7_3_RIGHT_3 1 - TTTTTGACTCTTATCACTGGAGGAACTAC +NC_001608.3 3645 3676 2ee06dc7_4_LEFT_1 2 + CCTTTCAAAAATTGCTTACAAATCAGGAAAG +NC_001608.3 3645 3676 2ee06dc7_4_LEFT_2 2 + CCTTTCAAAAATTGCTTACAAGTCAGGAAAA +NC_001608.3 3649 3676 2ee06dc7_4_LEFT_3 2 + TCAAAGATTGCTTACAAGTCAGGGAAA +NC_001608.3 4987 5016 2ee06dc7_4_RIGHT_1 2 - ACCATGTTCTGAATAAAAGCTTGATTTCC +NC_001608.3 4987 5017 2ee06dc7_4_RIGHT_2 2 - CACCATATTCTGAATAAAAGCTTGATTCCC +NC_001608.3 4987 5018 2ee06dc7_4_RIGHT_3 2 - TCACCATATTCTGAATAAAAGCTTGATTTCC +NC_001608.3 4987 5012 2ee06dc7_4_RIGHT_4 2 - TGTTTTGGACAAAAGCTTGGTTTCC +NC_001608.3 4815 4838 2ee06dc7_5_LEFT_1 1 + GCTGCCTCTTGGGATTATGAGCA +NC_001608.3 4817 4838 2ee06dc7_5_LEFT_2 1 + TGCCTCTTGGGATCATGAGCA +NC_001608.3 6179 6202 2ee06dc7_5_RIGHT_1 1 - CTTCCCCTTCCGTGTACTCAACA +NC_001608.3 6179 6205 2ee06dc7_5_RIGHT_2 1 - CTTCCTCCCCTTCTGTATACTCAACA +NC_001608.3 6179 6206 2ee06dc7_5_RIGHT_3 1 - GCTTCTTCTCCTTCCGTATACTCAACG +NC_001608.3 6179 6203 2ee06dc7_5_RIGHT_4 1 - TCCTCCCCTTCCGTATACTCAACA +NC_001608.3 6007 6032 2ee06dc7_6_LEFT_1 2 + AGATAGCCAGTAACAATCAACCCCA +NC_001608.3 6007 6032 2ee06dc7_6_LEFT_2 2 + AGATAGCCAGTAACATTCAACCCCA +NC_001608.3 6007 6032 2ee06dc7_6_LEFT_3 2 + AGATAGCTAGTAACAACCAACCCCA +NC_001608.3 6009 6032 2ee06dc7_6_LEFT_4 2 + ATAGCCAGTAACGATCAACCCCA +NC_001608.3 6006 6032 2ee06dc7_6_LEFT_5 2 + GAAATTGCTAGTAACAGCCAACCTCA +NC_001608.3 7386 7414 2ee06dc7_6_RIGHT_1 2 - AAGTTAAACTGATATTAGGGGAGGCATG +NC_001608.3 7386 7419 2ee06dc7_6_RIGHT_2 2 - AGATAAAGTTAAACTGATATTAGAGGAGGCATG +NC_001608.3 7386 7419 2ee06dc7_6_RIGHT_3 2 - AGATAAAGTTAAACTGATATTGGAGAATGCATG +NC_001608.3 7386 7418 2ee06dc7_6_RIGHT_4 2 - GATAAAGTTAAACTGATATTGGAGGATGCATG +NC_001608.3 7386 7412 2ee06dc7_6_RIGHT_5 2 - GTTAAACTGATATTCGGGGGAGTGTG +NC_001608.3 7386 7412 2ee06dc7_6_RIGHT_6 2 - GTTAAACTGATATTGGGGGAGGCATG +NC_001608.3 7261 7284 2ee06dc7_7_LEFT_1 1 + AAGGCGACATGTTCCCTTTTCTG +NC_001608.3 7262 7284 2ee06dc7_7_LEFT_2 1 + AGGCGACACGTTCCTTTTTCTG +NC_001608.3 7259 7284 2ee06dc7_7_LEFT_3 1 + GGAAGGCGACATATTCCCTTTTCTG +NC_001608.3 7259 7284 2ee06dc7_7_LEFT_4 1 + GGAAGGCGACATGTTCCCTTTTCTA +NC_001608.3 7254 7284 2ee06dc7_7_LEFT_5 1 + TGGAAAGAAGGTGATATATTCCCGTTTTTA +NC_001608.3 8551 8583 2ee06dc7_7_RIGHT_1 1 - AAGTTTATCACCTGGTAAATTAAAATGTAGGC +NC_001608.3 8551 8578 2ee06dc7_7_RIGHT_2 1 - CATGACCTAACGGATCAGATTTTAGGC +NC_001608.3 8551 8580 2ee06dc7_7_RIGHT_3 1 - CCCATGACTTAACGGATCAGATTTTAAGC +NC_001608.3 8551 8578 2ee06dc7_7_RIGHT_4 1 - CGTGACCTGACGGATCATATTTTAAGC +NC_001608.3 8551 8575 2ee06dc7_7_RIGHT_5 1 - GACCCAACGGATCAGATTTTAGGC +NC_001608.3 8551 8581 2ee06dc7_7_RIGHT_6 1 - GCCCATGACTTAACAGATCAGATTTTAAGC +NC_001608.3 8551 8577 2ee06dc7_7_RIGHT_7 1 - GTGACCTGACGGATCATATTTTGAGC +NC_001608.3 8280 8316 2ee06dc7_8_LEFT_1 2 + CTCTGAACCTAAGACCATAATAAATATTGGTTAAAG +NC_001608.3 8280 8316 2ee06dc7_8_LEFT_2 2 + CTCTGAACCTAAGACCATAATAAATATTGGTTAATG +NC_001608.3 8287 8316 2ee06dc7_8_LEFT_3 2 + CTTGAAGACATAGTTAAGACTGACCGAAA +NC_001608.3 8289 8316 2ee06dc7_8_LEFT_4 2 + TGAAGACATAGTTAAAACTGGCCGAAA +NC_001608.3 8289 8316 2ee06dc7_8_LEFT_5 2 + TGAAGACATAGTTAAGACTGGCCGAAA +NC_001608.3 9660 9690 2ee06dc7_8_RIGHT_1 2 - AGGAAGAATAAAATGATCGTATGAGGCTTG +NC_001608.3 9660 9690 2ee06dc7_8_RIGHT_2 2 - AGGAAGAATAAAATGGTCATATGAGGCTTG +NC_001608.3 9660 9689 2ee06dc7_8_RIGHT_3 2 - GGAAGAATAAAATGGTCGTATGAGGCTTG +NC_001608.3 9660 9688 2ee06dc7_8_RIGHT_4 2 - GGAGAATAAAGTGATCATATGAGGCCTG +NC_001608.3 9533 9560 2ee06dc7_9_LEFT_1 1 + CCTTGATAAAGGAGGTCAATTCGAAGC +NC_001608.3 9536 9560 2ee06dc7_9_LEFT_2 1 + TGACAAAGGAGGTCAATTCGAAGC +NC_001608.3 9536 9560 2ee06dc7_9_LEFT_3 1 + TGACAAAGGGGGTCAATTTGAAGC +NC_001608.3 10874 10897 2ee06dc7_9_RIGHT_1 1 - CCCTTAGGACTGCTTCAGCTACT +NC_001608.3 10874 10897 2ee06dc7_9_RIGHT_2 1 - CTCTTAGAACTGCCTCAGCCACT +NC_001608.3 10874 10899 2ee06dc7_9_RIGHT_3 1 - TTCTCTTAGAACTGCCTCAACCACT +NC_001608.3 10734 10765 2ee06dc7_10_LEFT_1 2 + AGCAGTATTGAAATAAAATTAACCGGACAAC +NC_001608.3 10734 10765 2ee06dc7_10_LEFT_2 2 + AGCAGTATTGAAATAAAGTTAACTGGACAAC +NC_001608.3 10735 10765 2ee06dc7_10_LEFT_3 2 + GCAGTATTGAAATAAAGTTAACTGGGCAAC +NC_001608.3 12095 12121 2ee06dc7_10_RIGHT_1 2 - ACTCTTAACCCTGGTTTTGAAGCTTG +NC_001608.3 12095 12120 2ee06dc7_10_RIGHT_2 2 - CCCTTAACCCTGGTTTTGAAGCTTG +NC_001608.3 12095 12119 2ee06dc7_10_RIGHT_3 2 - CCTCAACCCTGGTTTTGAAGCTTG +NC_001608.3 12095 12119 2ee06dc7_10_RIGHT_4 2 - CTTTAACCCTGGTTTTGAGGCCTG +NC_001608.3 12095 12117 2ee06dc7_10_RIGHT_5 2 - TCAGCCCTGGTTTTGAAGCTTG +NC_001608.3 11963 11993 2ee06dc7_11_LEFT_1 1 + ATAAAAGGACAAAGAGGGTCATTAAGAAGT +NC_001608.3 11968 11993 2ee06dc7_11_LEFT_2 1 + GGGACAAAGAGGGTCTTTAAGGAGC +NC_001608.3 13333 13360 2ee06dc7_11_RIGHT_1 1 - ACTATAGCGTTTTCCCCTATACTTGCT +NC_001608.3 13333 13357 2ee06dc7_11_RIGHT_2 1 - ATGGCATTCTCCCCTATACTTGCT +NC_001608.3 13333 13354 2ee06dc7_11_RIGHT_3 1 - GCGTTTTCCCCTATGCTTGCT +NC_001608.3 13192 13217 2ee06dc7_12_LEFT_1 2 + TCGTGTCAGAAATGTCCAAACACTC +NC_001608.3 13192 13217 2ee06dc7_12_LEFT_2 2 + TCGTGTCAGAAATGTCCAAACACTT +NC_001608.3 14559 14582 2ee06dc7_12_RIGHT_1 2 - AGCAAATCGGCTCATTACAGGGT +NC_001608.3 14559 14581 2ee06dc7_12_RIGHT_2 2 - GCAAATCGGCTCATTACGGGGT +NC_001608.3 14424 14455 2ee06dc7_13_LEFT_1 1 + AAATAATAACGTTCCTTAGACAAACAGTTCG +NC_001608.3 14428 14455 2ee06dc7_13_LEFT_2 1 + AATAACGTTCCTTAGACAGACAGTTCG +NC_001608.3 14432 14455 2ee06dc7_13_LEFT_3 1 + ACGTTTCTCAGGCAAACAGTTCG +NC_001608.3 15799 15821 2ee06dc7_13_RIGHT_1 1 - CCACGCAGCAATAGTCGGAAAG +NC_001608.3 15799 15824 2ee06dc7_13_RIGHT_2 1 - TGTCCATGCAGCAATAGTTGGAAAG +NC_001608.3 15649 15682 2ee06dc7_14_LEFT_1 2 + ACCTTTAGATGTAGATTTGAACAAGTATATGGA +NC_001608.3 15655 15682 2ee06dc7_14_LEFT_2 2 + AGACGTGGATCTGAACAAGTATATGGA +NC_001608.3 15650 15682 2ee06dc7_14_LEFT_3 2 + CCTTTAGATGTGGATTTGAACAAGTATATGGA +NC_001608.3 15651 15682 2ee06dc7_14_LEFT_4 2 + CTTTAGATGTGGATCTGAACAAGTATATGGA +NC_001608.3 15653 15682 2ee06dc7_14_LEFT_5 2 + TTAGATGTGGATTTGAACAAGTACATGGA +NC_001608.3 17025 17057 2ee06dc7_14_RIGHT_1 2 - AGTTGATGAATATCTAGAGAGTTGTAATGGAC +NC_001608.3 17025 17051 2ee06dc7_14_RIGHT_2 2 - CAGACACTCAGAAGGTTGCAATAGAC +NC_001608.3 17025 17053 2ee06dc7_14_RIGHT_3 2 - GATGAATACCTAGAGGATTGTAATGGGC +NC_001608.3 17025 17056 2ee06dc7_14_RIGHT_4 2 - GTTGATGAATATCTAGAGGGTTGTAATGGAC +NC_001608.3 17025 17054 2ee06dc7_14_RIGHT_5 2 - TGATGAATACCTAAAGGATTGTAATGGGC +NC_001608.3 17025 17054 2ee06dc7_14_RIGHT_6 2 - TGATGAATATCTAGAGGATCGTAACGGAC +NC_001608.3 16894 16920 2ee06dc7_15_LEFT_1 1 + AGATGTACATGAGGACTTTGATGCCT +NC_001608.3 16897 16920 2ee06dc7_15_LEFT_2 1 + CATGCTTAGGAATTGTGACGCCA +NC_001608.3 16896 16920 2ee06dc7_15_LEFT_3 1 + GCATGCTTAGAAATTGTGACGCCA +NC_001608.3 16895 16920 2ee06dc7_15_LEFT_4 1 + GGTGTACATGAGGACTTTGATGCCT +NC_001608.3 18151 18181 2ee06dc7_15_RIGHT_1 1 - GAGCATATGAAGTGATTTCTCAGATCTAGG +NC_001608.3 18151 18182 2ee06dc7_15_RIGHT_2 1 - TGAGCAAATAAAGTGATTTCTCAAGTCTAGT +NC_001608.3 17337 17362 2ee06dc7_16_LEFT_1 2 + GAAGTGGTGCTCGGTTACTTTTGAA +NC_001608.3 17340 17362 2ee06dc7_16_LEFT_2 2 + GTGGCGCTCGACTACTCTTGAA +NC_001608.3 17340 17362 2ee06dc7_16_LEFT_3 2 + GTGGTGCTCGGTTACTCCTGAA +NC_001608.3 18661 18688 2ee06dc7_16_RIGHT_1 2 - AACGTTCAATCACAAGTTAATCTGTGC +NC_001608.3 18661 18687 2ee06dc7_16_RIGHT_2 2 - ACATCCAATCACAAGTTGATCTGTGC +NC_001608.3 18661 18681 2ee06dc7_16_RIGHT_3 2 - AGCGGTCAGTTCGGCTATGT +NC_001608.3 18661 18687 2ee06dc7_16_RIGHT_4 2 - CCGTTCAACCACAAGTTAATCTGTGT +NC_001608.3 18661 18690 2ee06dc7_16_RIGHT_5 2 - CGAACATCCAATTACAAGTTAATCTGTGC +NC_001608.3 18661 18690 2ee06dc7_16_RIGHT_6 2 - CGAACGTTCAATCACAAGTTAATCTGTGT +NC_001608.3 18661 18689 2ee06dc7_16_RIGHT_7 2 - GAACATCCAATCACAAGTTAATCTGTGC +NC_001608.3 18661 18689 2ee06dc7_16_RIGHT_8 2 - GAACGTTCAATCATAAGTTAATCTGCGC diff --git a/primerschemes/artic-marv/1200/v1.0.0/reference.fasta b/primerschemes/artic-marv/1200/v1.0.0/reference.fasta new file mode 100644 index 0000000..93703c5 --- /dev/null +++ b/primerschemes/artic-marv/1200/v1.0.0/reference.fasta @@ -0,0 +1,320 @@ +>NC_001608.3 Marburg marburgvirus isolate Marburg virus/H.sapiens-tc/KEN/1980/Mt. Elgon-Musoke, complete genome +AGACACACAAAAACAAGAGATGATGATTTTGTGTATCATATAAATAAAGAAGAATATTAA +CATTGACATTGAGACTTGTCAGTCTGTTAATATTCTTGAAGAGATGGATTTACACAGTTT +GTTGGAGTTGGGTACAAAACCCACTGCCCCTCATGTTCGTAATAAGAAAGTGATATTATT +TGACACAAATCATCAGGTTAGTATCTGTAATCAGATAATAGATGCAATAAACTCAGGGAT +TGATCTTGGAGATCTCCTAGAAGGGGGTTTGCTGACGTTGTGTGTTGAGCATTACTATAA +TTCTGATAAGGATAAATTCAACACAAGTCCTATCGCGAAGTACTTACGTGATGCGGGCTA +TGAATTTGATGTCATCAAGAATGCAGATGCAACCCGCTTTCTGGATGTGATTCCTAATGA +ACCTCATTACAGCCCTTTAATTCTAGCCCTTAAGACATTGGAAAGTACTGAATCTCAGAG +GGGGAGAATTGGGCTCTTTTTATCATTTTGCAGTCTTTTCCTCCCAAAACTTGTCGTCGG +AGACCGAGCTAGTATCGAAAAGGCTTTAAGACAAGTAACAGTGCATCAAGAACAGGGGAT +CGTCACATACCCTAATCATTGGCTTACCACAGGCCACATGAAAGTAATTTTCGGGATTTT +GAGGTCCAGCTTCATTTTAAAGTTTGTGTTGATTCATCAAGGAGTAAATTTGGTGACAGG +TCATGATGCCTATGACAGTATCATTAGTAATTCAGTAGGTCAAACTAGATTCTCAGGACT +TCTTATCGTGAAAACAGTTCTCGAGTTCATCTTGCAAAAAACTGATTCAGGGGTGACACT +ACATCCTTTGGTGCGGACCTCCAAAGTAAAAAATGAAGTTGCTAGTTTCAAGCAGGCGTT +GAGCAACCTAGCCCGACATGGGGAATACGCACCATTTGCACGGGTTCTGAATTTATCAGG +GATTAACAACCTCGAACATGGACTCTATCCTCAGCTTTCAGCAATTGCGCTGGGTGTGGC +AACAGCACACGGCAGTACATTGGCTGGTGTCAATGTTGGCGAACAATATCAACAACTACG +AGAGGCGGCACATGATGCGGAAGTAAAACTACAAAGGCGACATGAACATCAGGAAATTCA +AGCTATTGCCGAGGATGACGAGGAAAGGAAGATATTAGAACAATTCCACCTTCAGAAAAC +TGAAATCACACACAGTCAGACACTAGCCGTCCTCAGCCAGAAACGAGAAAAATTAGCTCG +TCTCGCTGCAGAAATTGAAAACAATATTGTGGAAGATCAGGGATTTAAGCAATCACAGAA +TCGGGTGTCACAGTCGTTTTTGAATGACCCTACACCTGTGGAAGTAACGGTTCAAGCCAG +GCCCATGAATCGACCAACTGCTCTGCCTCCCCCAGTTGACGACAAGATTGAGCATGAATC +TACAGAAGATAGCTCTTCTTCAAGTAGCTTTGTTGACTTGAATGATCCATTTGCACTGCT +GAATGAGGACGAGGATACTCTTGATGACAGTGTCATGATCCCGGGCACAACATCGAGAGA +ATTTCAAGGGATTCCTGAACCGCCAAGACAATCCCAAGACCTCAATAACAGCCAAGGAAA +GCAGGAAGATGAATCCACAAATCCGATTAAGAAACAGTTTCTGAGATATCAAGAATTGCC +TCCTGTTCAAGAGGATGATGAATCGGAATACACAACTGACTCTCAAGAAAGCATCGACCA +ACCAGGATCCGACAATGAACAAGGAGTTGATCTTCCACCTCCTCCGTTGTACGCTCAGGA +AAAAAGACAGGACCCAATACAGCACCCAGCAGCAAACCCTCAGGATCCCTTCGGCAGTAT +TGGTGATGTAAATGGTGATATCTTAGAACCTATAAGATCACCTTCTTCACCATCTGCTCC +TCAGGAAGACACAAGGATGAGGGAAGCCTATGAATTGTCGCCTGATTTCACAAATGATGA +GGATAATCAGCAGAATTGGCCACAAAGAGTGGTGACAAAGAAGGGTAGAACTTTCCTTTA +TCCTAATGATCTTCTGCAAACAAATCCTCCAGAGTCACTTATAACAGCCCTCGTTGAGGA +ATACCAAAATCCTGTCTCAGCTAAGGAGCTTCAAGCAGATTGGCCCGACATGTCATTTGA +TGAAAGGAGACATGTTGCGATGAACTTGTAGTCCAGATAACACAGCACGGTTACTCACTT +ATCTATTTTGATATGACTCATCCTCAGATCACAGCAATCAAATTTATTTGAATATTTGAA +CCACCTTTTAGTATCCTATTACTTGTTACTATTGTGTGAGACAACATAAGCCATCAAATA +ACAATCACGGGCAAGGACTGGGCATACTATGGTGGTCTTAGAGCATTGTCCAGTGCTACA +AATTCTTTTTTCAATTGCTATAATTATACAACTACAAACCTCCATACATTTGCCGCAACA +CTGTAATCAACACTGCTGTATCTCTTCTTCAAGCCATCTGATTTAACTTAATAAACATGA +CTTGATTCAAAGAATATACTGACAATGTTACTGTTTGAATTTCTCAAGTGGTGCACTATC +CTACTGTTTTGCTCAGCTTAGTATATTGTAATATGTAAGTGGACTCTCCCCTTCTCCTCT +CGTGTATTCTTTATAAATCACTTACTTGATAGAATGTCGAGTCTACTGGTTTGGAGTTTC +CTTACTCTAATGGATGTAATAATTAACTGTTGGCCTAGATGATAACAGATATGAGGTTAT +ATAATTACTCATAGTGTAAAGTATAATTCTTACCTCTGTTTCTTCTGTTTTCCCTTTCTT +TTATAATATGCCAATTAAGAAAAACTAAAAATCGAAGAATATTAAAGATTTTCTTTAATA +TTCAGAAAAGGCTTTTTATTCTATTCTTTCTTTTTACAAACGTATTGAAATAGTAATTCT +CACAATGTGGGACTCATCATACATGCAGCAAGTCAGCGAAGGGTTGATGACTGGAAAAGT +ACCCATAGATCAAGTGTTTGGTGCCAATCCCTTAGAGAAGTTATACAAGAGAAGAAAACC +AAAAGGCACAGTTGGACTACAATGTAGCCCTTGTCTAATGTCAAAGGCGACAAGTACTGA +TGATATTATTTGGGACCAACTGATCGTGAAGAGAACACTAGCTGATCTACTTATACCGAT +AAATAGGCAGATATCAGACATTCAAAGCACTCTAAGCGAAGTAACAACAAGAGTCCATGA +AATTGAGCGGCAATTACATGAGATTACCCCAGTTTTAAAAATGGGAAGGACACTGGAAGC +AATTTCCAAGGGGATGTCAGAAATGTTAGCCAAATACGACCACCTTGTAATTTCAACTGG +AAGAACCACTGCACCAGCTGCTGCCTTTGATGCCTACTTAAATGAGCATGGTGTCCCTCC +CCCTCAACCCGCGATTTTCAAAGATCTTGGGGTTGCCCAACAAGCTTGTAGTAAGGGGAC +CATGGTTAAAAATGCAACAACAGATGCAGCCGACAAGATGTCGAAGGTTCTTGAACTCAG +TGAGGAAACGTTCTCCAAGCCAAACCTTTCAGCTAAGGATTTAGCCCTTTTATTGTTTAC +CCATCTACCCGGCAACAACACTCCATTCCATATCCTAGCTCAGGTCCTTTCAAAAATTGC +TTACAAGTCAGGAAAATCCGGAGCATTCTTGGATGCATTTCACCAGATTCTAAGTGAAGG +AGAGAATGCTCAGGCGGCATTAACTCGACTAAGCAGAACATTTGACGCTTTCCTTGGAGT +GGTTCCTCCAGTGATAAGAGTCAAAAACTTCCAAACAGTCCCTCGTCCATGTCAAAAAAG +TCTTCGGGCTGTCCCTCCAAATCCAACAATTGACAAAGGATGGGTCTGTGTTTATTCATC +TGAGCAAGGTGAAACACGGGCCCTTAAAATCTAATTCTCATTGTTCATAGTTGCAAGGGA +AGTGATCTTTCCGAGTTGATACAAAGACACTAAACATTTCAAAAGCATGTATGTGGACAA +AACATAATTAGACCATCTTAATTGGAGTAGTAATTTATTTCTGTCTTAAATGTGATTTTC +ACTTTAAAAGCGTTAAATGGTGATAGATTAATCCTTGAAGTTACTCTTCTATATATTATA +GAGAAACCAATGTTACTAACAAAAGGGGTCTACCTAACGCATATGATTGAGTAATCCGTA +TATTTTATAAACCAAACAATTAACTTCTTACTTTTTAAGAATCAACTAACAACATAGAAA +AGACATTTATCCTTATGTAATCCTCGGCTTAGTTGAAATTAACTTTTGTTGGACCTCAAG +ACGCTTATTCATAGTATATTATATGATTTTTTATAAGTTTAAGATATCTTAAATTATACC +CACAAAAGATACTGTTTTAATTAAGAAAAACTATGAAGAACATTAAGAAGATCTTTCTTT +CGTAGTGTTCTTTTACTGGAAGGAGTATTCCAATTTCAGCTTGTTGGATTAATTGTTACT +TAAATTGTCCTTTTTGAAATTAATTCACACAAGGTAGTTTAAATTTATATCCAAAATAAA +TTTTGATATGGCCAGTTCCAGCAATTACAACACATACATGCAATACTTGAACCCCCCTCC +TTATGCTGATCACGGTGCAAACCAGTTGATCCCGGCGGATCAGCTATCAAATCAGCAGGG +TATAACTCCAAATTACGTGGGTGATTTAAACCTAGATGATCAGTTCAAAGGGAATGTCTG +CCATGCTTTCACTTTAGAGGCAATAATTGACATATCTGCATATAACGAGCGAACAGTCAA +AGGCGTTCCGGCATGGCTGCCTCTTGGGATTATGAGCAATTTTGAATATCCTTTAGCTCA +TACTGTGGCCGCGTTGCTCACAGGCAGCTATACAATCACCCAATTTACTCACAACGGGCA +AAAATTCGTCCGTGTTAATCGACTTGGTACAGGAATCCCAGCACACCCACTCAGAATGTT +GCGTGAAGGAAATCAAGCTTTTATTCAGAATATGGTGATCCCCAGGAATTTTTCAACTAA +TCAATTCACCTACAATCTCACTAATTTAGTATTGAGTGTGCAAAAACTTCCTGATGATGC +CTGGCGCCCATCCAAGGACAAATTAATTGGGAACACTATGCATCCCGCAGTCTCCATCCA +CCCGAATCTGCCGCCTATTGTTCTACCAACAGTCAAGAAGCAGGCTTATCGTCAGCACAA +AAATCCCAACAATGGACCATTGCTGGCCATATCTGGCATCCTCCATCAACTGAGGGTCGA +AAAAGTCCCAGAGAAGACGAGCCTGTTTAGGATCTCGCTTCCTGCCGACATGTTCTCAGT +AAAAGAGGGTATGATGAAGAAAAGGGGAGAAAATTCCCCCGTGGTTTATTTTCAAGCACC +TGAGAACTTCCCTTTGAATGGCTTCAATAACAGACAAGTTGTGCTAGCGTATGCGAATCC +AACGCTCAGTGCCGTTTGAAATGATGCTCAAATGAGACAGGAGTCCATCTGTATAAGAAG +TATGGCTTAAATGGATATTTGTCAAATTCTTACAAGATTAGTTTGTATTGATTTCAACAA +TGCTTTAACCTTACATTGCTGCTTTAAATAGTTGATTAAGCTGATCAGCTTGTAATATGT +AATCTCTTCTGGGCCATCAGATCCATAATGGGTTTACTAGACTATATAAGAGAAATAGTA +ATATTTTATAAACAATTCTTGCTCAGTTTTACTGTGATTTAATAACATATGTCATTGTGC +CCTCCATTGCTAAGTCAACTCAACTGACGATAATACTCCTTCTGAAATAGTAAGAAAAAC +TAATGAAGAACATTAATTGCTGGGTAAAAGTGATTAATTTCTTTAAATTTGACCAGAATA +ATATTTTGTCAGTGAATATATTCTCATATCACTTGATTAAAAACAGAAAATTACCCTAAC +ATGAAGACCACATGTTTCCTTATCAGTCTTATCTTAATTCAAGGGACAAAAAATCTCCCC +ATTTTAGAGATAGCTAGTAATAATCAACCCCAAAATGTGGATTCGGTATGCTCCGGAACT +CTCCAGAAGACAGAAGACGTCCATCTGATGGGATTCACACTGAGTGGGCAAAAAGTTGCT +GATTCCCCTTTGGAGGCATCCAAGCGATGGGCTTTCAGGACAGGTGTACCTCCCAAGAAT +GTTGAGTACACAGAGGGGGAGGAAGCCAAAACATGCTACAATATAAGTGTAACGGATCCC +TCTGGAAAATCCTTGCTGTTAGATCCTCCTACCAACATCCGTGACTATCCTAAATGCAAA +ACTATCCATCATATTCAAGGTCAAAACCCTCATGCACAGGGGATCGCCCTTCATTTATGG +GGAGCATTTTTTCTGTATGATCGCATTGCCTCCACAACAATGTACCGAGGCAAAGTCTTC +ACTGAAGGGAACATAGCAGCTATGATTGTCAATAAGACAGTGCACAAAATGATTTTCTCG +CGGCAAGGACAAGGGTACCGTCATATGAATCTGACTTCTACTAATAAATATTGGACAAGT +AGTAACGGAACGCAAACGAATGACACTGGATGTTTCGGCGCTCTTCAAGAATACAATTCT +ACAAAGAACCAAACATGTGCTCCGTCCAAAATACCTCCACCACTGCCCACAGCCCGTCCG +GAGATCAAACTCACAAGCACCCCAACTGATGCCACCAAACTCAATACCACGGACCCAAGC +AGTGATGATGAGGACCTCGCAACATCCGGCTCAGGGTCCGGAGAACGAGAACCCCACACA +ACTTCTGATGCGGTCACCAAGCAAGGGCTTTCATCAACAATGCCACCCACTCCCTCACCA +CAACCAAGCACGCCACAGCAAGGAGGAAACAACACAAACCATTCCCAAGATGCTGTGACT +GAACTAGACAAAAATAACACAACTGCACAACCGTCCATGCCCCCTCATAACACTACCACA +ATCTCTACTAACAACACCTCCAAACACAACTTCAGCACTCTCTCTGCACCATTACAAAAC +ACCACCAATGACAACACACAGAGCACAATCACTGAAAATGAGCAAACCAGTGCCCCCTCG +ATAACAACCCTGCCTCCAACGGGAAATCCCACCACAGCAAAGAGCACCAGCAGCAAAAAA +GGCCCCGCCACAACGGCACCAAACACGACAAATGAGCATTTCACCAGTCCTCCCCCCACC +CCCAGCTCGACTGCACAACATCTTGTATATTTCAGAAGAAAGCGAAGTATCCTCTGGAGG +GAAGGCGACATGTTCCCTTTTCTGGATGGGTTAATAAATGCTCCAATTGATTTTGACCCA +GTTCCAAATACAAAAACAATCTTTGATGAATCCTCTAGTTCTGGTGCCTCGGCTGAGGAA +GATCAACATGCCTCCCCCAATATTAGTTTAACTTTATCTTATTTTCCTAATATAAATGAG +AACACTGCCTACTCTGGAGAAAATGAGAATGATTGTGATGCAGAGTTAAGAATTTGGAGC +GTTCAGGAGGATGACCTGGCCGCAGGGCTCAGTTGGATACCGTTTTTTGGCCCTGGAATT +GAAGGACTTTACACTGCTGTTTTAATTAAAAATCAAAACAATTTGGTCTGCAGGTTGAGG +CGTCTAGCCAATCAAACTGCCAAATCCTTGGAACTCTTATTGAGAGTCACAACTGAGGAA +AGAACATTCTCCTTAATCAATAGACATGCTATTGACTTTCTACTCACAAGATGGGGAGGA +ACATGCAAAGTGCTTGGACCTGATTGTTGCATCGGGATAGAAGACTTGTCCAAAAATATT +TCAGAGCAAATTGACCAAATTAAAAAGGACGAACAAAAAGAGGGGACTGGTTGGGGTCTG +GGTGGTAAATGGTGGACATCCGACTGGGGTGTTCTTACTAACTTGGGCATTTTGCTACTA +TTATCCATAGCTGTCTTGATTGCTCTATCCTGTATTTGTCGTATCTTTACTAAATATATC +GGATAACGTTAAATGTGTAATGATTAGGACTTTAGGACAATTGCTACTGAGCCCTTTTTC +TAATCTACTGAAATCAACTTGGGAGATTTTTAAGAAGCTGATAACTTAATGTGAATCAAT +AGTTTATGTATTATCGATTATTATGGTTTGATATTCAATTGTTATTATTGTCAGGAGTGA +CCTTTTCTATTTGATGCATTAATGTTTTAAACTACCTCTTAAGCCTTTGAGGGCGGTCCC +AATATGTGCGTAGGGGTTAATTTAAAGGGATTTCTTATTGTACAGTTTTCTGTATTACTT +ATTTGGGCTTGAAGACATAGTTAAGATTTGCCGAAAATGCTCTCCAGTCAATTCCATCCC +CTCTCAGAAAAGACGTGCTGTTCAAAGAGTCTTAATTTATAACCAACTATTGCAAGAATT +AATTTACTTTTTCCGTTATACTTAGTTACATTAATCTTTTGACTGTTCAGCATTATTAAC +GACTTGTCTTAATTCAATCGTTCGGATGAAATTCATAAGGAAAAATGAGCCTCCTTCCCC +CTATTCTGGGCTGAGAAAATTTCTCTTATCCGCCTAAAATCTGATCTGTTAGGTCATGGG +TCCTTCATAATCTGTTTGAGCATGAATATTGATCAAATGACCAAATGATAGTGCATTTGT +ATAGACTCAATTATCCTTTATTAAGAAAAAGATAAATAGAACACAAAGAATTGACAAAAT +TTTACTTTGATTGATTTTGCAAGGAGTTATAAAAATCTTGAAGGATAAATTGTTATAAAG +TAGAGTCGAAGAACATTAAATGTTCTTTGTTAGAATTATTCATCTAAGTTGTTTTTGAGT +ATATTCGCTTCAATACAACTCCTCTTTATATTTGATTTAAGTTTTAAAATGCAACAACCT +CGCGGAAGAAGTCGAACCCGCAACCACCAAGTCACACCGACTATATATCATGAAACTCAA +TTGCCCTCCAAACCTCATTATACCAATTATCATCCACGTGCAAGATCGATGAGCTCAACC +CGTAGTAGTGCAGAAAGTAGTCCCACCAATCATATTCCCCGTGCTCGACCACCCTCAACA +TTCAACTTATCGAAACCCCCTCCTCCTCCAAAAGACATGTGCAGGAACATGAAAATTGGA +TTGCCGTGCGCTGATCCCACTTGTAATAGAGATCATGACCTTGATAATCTAACAAATCGT +GAACTTTTGCTATTGATGGCCCGAAAAATGCTCCCCAATACAGACAAGACCTTTAGAAGT +CCGCAGGACTGTGGATCACCGTCTCTTTCTAAAGGTCTCTCAAAAGATAAACAGGAGCAA +ACGAAAGATGTGTTGACCTTGGAAAATCTAGGACACATTCTGAGCTATCTCCACAGATCA +GAAATTGGGAAATTGGATGAGACATCACTTCGTGCAGCATTAAGTCTGACGTGTGCTGGA +ATTCGAAAGACGAATAGATCCTTGATCAACACCATGACAGAATTACACATGAACCATGAA +AATCTCCCGCAAGACCAAAACGGTGTTATCAAGCAGACCTATACAGGTATTCACCTTGAC +AAAGGAGGTCAATTCGAAGCCGCCTTATGGCAAGGTTGGGATAAGAGATCGATATCTCTA +TTCGTACAAGCAGCTTTATATGTAATGAACAATATCCCCTGTGAATCATCAATCAGTGTG +CAAGCCTCATACGATCATTTTATTCTTCCTCAAAGTCAAGGTAAAGGACAGTGATTATTG +TTCGAAAGTTGACAATTTGATCACTTTCAGTTTTCAGTTTCAACCCTTATCGCGAGACTT +GAATACAATCCTACTAACTTCAATAAGTGACCCCAAATTCAAGTTTGCTGAAAGCTAAGA +TGACAATGATCACTAGTTCATTGTAAATTACTCGATCAAAATGTTCTTAAGCTATCTTAA +GCTTACTGATGCGGCTCTGCTTCACTTTTCTTTTGATTTTAAAGCCATAGCTATATCTAA +GTGTCTAATTAACAACTTGTACCTCTAAGGAAAAACATGAAGAACATTAAGAAAAAGGAT +GTTCTTATTCTTTGACTAAACCTGCATATTCTTTGTTGATACCCTCGAGAGACAACTTTT +GACACCAGATCACGGATCAAGCACACTTCAATCAAGCACCCTAAATTTTCAATCATACAC +ATAATAACCATTTTAGTAGCGTTGCCTTTCAGTACAGTCTAGGTGATTGTTGAAAGACTT +CCAAGCATGGCAGAATTATCAACGCGTTACAACTTGCCTGCAAATGTTACGGAAAATAGT +ATAAATCTTGACCTTAATTCCACAGCACGATGGATAAAAGAACCCAGTGTTGGGGGCTGG +ACAGTGAAGTGGGGAAACTTTGTTTTCCATATACCAAATACTGGAATGACATTGTTGCAT +CATTTAAAGTCTAACTTCGTTGTTCCAGAGTGGCAACAAACAAGGAATCTATTCTCCCAC +CTCTTTAAAAACCCAAAATCAACAATTATAGAACCGTTTTTGGCCCTGAGGATTTTGCTT +GGAGTTGCTTTGAAGGATCAAGAATTACAGCAATCATTGATTCCTGGATTTAGATCTATT +GTTCATATGCTATCAGAATGGCTGCTCCTGGAGGTCACGTCGGCAATCCATATTAGCCCT +AATCTGTTGGGAATCTATTTGACTTCAGACATGTTTAAAATTCTGATGGCAGGTGTGAAA +AATTTCTTCAATAAGATGTTCACTCTTCATGTTGTAAATGACCACGGAAAACCCAGCAGT +ATTGAAATAAAGTTAACTGGACAACAGATCATTATCACTCGTGTTAATATGGGGTTTCTA +GTGGAAGTCAGGAGGATTGATATTGAACCTTGCTGTGGTGAGACAGTCCTCTCAGAATCA +GTTGTTTTTGGACTAGTGGCTGAGGCAGTTCTAAGAGAACACAGTCAAATGGAGAAGGGC +CAACCTCTCAATCTGACACAATACATGAACAGCAAAATTGCTATATAAGTGGCTTAAATT +AGCATGGGTATTCCTAGTTCGACCACATAATAATGTTGGAGGCACAGTACATTATAGTTA +ATTGTCTTGTATACTAAGGGATATACCTAACCTGATTTATATTTACTGGTATAAAATAGT +AGCATCATCTTATTGAATAGTTATCATACAATAGGCTGTTCCTATAATCTGATTGTGAGA +TTATAAACTTGTAGAATTACCGTGGGTCACAACTGTTGCATATCCTCCAAAATATATCTT +TTGCAAGTGATGTGTGCTTGAATACTTCGATATAATACATACTAATAACGATTGATTAAG +AAAAATCAATGATGGATATTAAATGTCCATCAAGCAAGTGTTGTAGAATACCAGGGGTTT +CACAGGCTGCTAAACTTACTAAATTTTACATAGGATTATATAATTCTTTTCGATACACGT +TATATCTTTAGCAAAGTGAGGAAAACAGCTTTATCATGTTAGATGCCAGTTATCCATTTT +AAGTGAATCCTTTCCTCAACATGCAGCATCCAACTCAATATCCTGATGCAAGGTTGTCCT +CTCCTATAATCCTAGACCAGTGTGACTTATTAGCCAGAAGTTTAGGGTTGTATAGTCATT +ATTCACATAATCCGAAATTGCGTAATTGTAGGATTCCACATCACATTTACCGTTTAAGGA +ATTCGACAGCATTAAAGACATTTCTTCAGAATTGTTCGATACTCACCGTTCCTTTTCATT +CAATTTGGGATCATATTTTAACTTCCATTCAATATGATGCAATTAATCATGTTGATGATT +TTAAATACCTATTGCCATCTGAGCTAGTCAAGTATGCGAATTGGGACAACGAGTTCTTAA +AAGCATATCTTAATAAGATCTTAGGACTTGACCATGTTTTTTCAGCTTCTGCAAGGTCAC +AGTGTGAGGATTTTTCTCCTAAGGAAAATCCTTATTATTGGGGGATGTTATTACTCGTGC +ATCTATCTCAACTTGCCAGGAGGATAAAAGGACAAAGAGGGTCATTAAGAAGTAACTGGA +AATTTATAGGAACAGATTTGGAGCTGTTTGGAATAGCAGATTTTGTTATTTTTAAGGTTC +CAGTAAAAACAATAATCCGAAATGCTGTAAGCTTACAAGCTTCAAAACCGGGATTAAGAA +TATGGTACCGTGACCAAAACTTGACCCCTTATCTATGCGACGATGAGTTTATTGTAAGCG +TCGCTAGTTATGAATGTTTTATCATGATTAAAGACGTCTTCATTGAGAGGTATAACACAT +GGGAAATCTGTGCCCGCGCTTGGCTCGAAGACAGTGATGGAGCTGATTATCCTCCTCTTG +ATGTGTTAGGTGAATTATACAACCAGGGAGACCAAATTATTGCCATGTACCTGGAGGACG +GTTTCAAATTGATTAAACACTTAGAACCCTTGTGTGTCAGCTGTATACAAACACATGGCA +TCTTTACACCAAGAAAATACTGGTTCCAATCACAGATGATTAAGTCATATTATGATGAAC +TCCATGATCTCAATTTGAAACTTCAAATTTCAGACAATAAGGCTGAGTGTGCCCAAAACT +TTATTAAAACTATAGTTCAGGCGAAATTGACTCCTCAACAATACTGTGAATTATTCTCCC +TACAAAAGCATTGGGGTCATCCCGTTTTATACAATGATGTTGCACTAGATAAGGTTAAAA +AACATGCGCAATCGACAAAAATCTTAAAACCTAAAGTCATGTTTGAAACTTTTTGTGTTT +TCAAATTTATAGTAGCAAAGAATCATTATCATTCTCAAGGATCATGGTATAAAACCACAC +ATGATTTGCATTTGACTCCATATCTTAGACAACATATTGTGTCAAATTCATTTCCATCAC +AAGCCGAAATTTATCAGCATCTTTGGGAGTGGTATTTCGTGGAGCATGAACCTCTTTTCT +CAACTAAAATAATAAGTGATTTAAGTATCTTTATAAAAGACAGGGCTACCGCTGTGAACC +AGGAGTGTTGGGACAGTGTCTTCGATAGAAGTGTATTAGGATATAACCCTCCTGTTAGAT +TTCAATCAAAGAGAGTGCCAGAGCAATTTTTGGGTCAAGCAGACTTTTCCTTGAATCAAA +TATTAGAGTTTGCTGAAAAGTTAGAGTATTTGGCTCCTTCTTATAGGAATTTTTCCTTCT +CATTAAAAGAAAAAGAGTTGAATATAGGAAGAACTTTTGGGAAGTTACCGTATCGTGTCA +GAAATGTCCAAACACTCGCAGAAGCCCTGCTAGCAGATGGACTGGCAAAAGCATTCCCTA +GTAACATGATGGTTGTCACTGAGAGGGAACAGAAAGAAGCATTATTACATCAGGCTTCTT +GGCACCACAATTCAGCAAGCATAGGGGAGAACGCTATAGTGAGGGGTGCAAGTTTTGTTA +CTGATCTTGAGAAATACAACCTCGCCTTCCGATATGAATTTACACGGCATTTCATAGACT +ACTGTAATCGATGTTATGGTGTGAAGAATTTATTTGATTGGATGCATTTTTTAATACCAC +TATGTTATATGCATGTCAGTGACTTTTATAGCCCACCACATTGTGTGACAGAAGATAATC +GAAATAACCCACCTGATTGTGCTAATGCTTATCATTATCACTTAGGAGGTATAGAGGGAC +TTCAGCAGAAATTGTGGACATGCATATCATGTGCCCAAATCACCCTTGTGGAGTTAAAAA +CTAAATTAAAATTGAAGTCCAGCGTCATGGGTGATAATCAATGTATAACAACTCTAAGTC +TTTTTCCAATTGATGCTCCCAACGATTATCAAGAGAACGAGGCTGAATTGAATGCGGCAC +GAGTTGCTGTCGAATTAGCTATTACTACAGGTTATAGTGGTATATTTTTAAAGCCTGAGG +AAACATTTGTCCATTCAGGGTTCATTTATTTTGGTAAAAAGCAATATCTCAACGGTGTTC +AACTGCCGCAATCATTGAAAACAATGGCAAGGTGTGGACCCTTATCTGACTCTATTTTCG +ATGATCTTCAAGGTTCTCTGGCCAGTATTGGTACATCCTTTGAGAGAGGAACAAGCGAGA +CACGGCACATTTTTCCGAGCCGTTGGATAGCTTCATTCCATTCAATGTTAGCAATAAATT +TATTAAATCAGAATCACCTTGGGTTTCCTCTAGGGTTCAATATTGATATTTCTTGTTTCA +AAAAGCCTCTTACCTTCTCGGAAAAATTAATTGCTCTCATAACGCCCCAAGTTTTAGGAG +GGTTATCATTTTTAAATCCAGAAAAATTGTTCTACCGGAACATAAGTGATCCTCTCACTT +CAGGTCTATTTCAACTCAAGAATGCATTGGAATTTCTTGAAAAGGAAGAATTATTCTATA +TCTTGATTTCTAAAAAACCTGGTTTAGCAGATGCCTCAGATTTTGTCATGAATCCATTAG +GCTTAAATGTACCAGGATCAAAGGAAATAATAACGTTCCTTAGACAAACAGTTCGCGAAA +ATATCACGATCACGTCACAAAATAGAATAATAAATTCTCTTTTCCACATAGGTTCTGATT +TAGAGGACCAAAGAGTGTGTGAGTGGCTTTTATCATCAAACCCTGTAATGAGCCGATTTG +CTGCTGACATCTTTTCAAGAACACCTAGTGGAAAACGGCTTCAAGTCTTAGGCTATCTAG +AAGGAACAAGAACATTACTAGCTTCTCGGACAATCAGTTTAACTACAGAAGGAACAATGT +TGATGAAATTAAGAGAATTAACGAGAAACCGATGGAAAAGTTGGTTTTCTTATATTGATG +CACTGGACGATGATTTATCTGAGTCCTTGGAAAAGTTCACATGTACTGTTGATGTGGCTA +ATTTCTTGAGGGCATATTCATGGTCTGACGTCTTAAAAGGGAAAAGGCTAATTGGTGCCA +CACTGCCATGTTTACTAGAGCAATTTGAGGTAAAGTGGATTAATTTATCTGAGGATTTAA +GGGAACAATTTAATCTATCTTCAGACTCAAAATCAACTATAAACTTGTTGCCGTATGACT +GTAAGGAACTGCGACTTGAAGGAAGCAATGACACAGAGTTAAATTATGTCAGTTGTGCTC +TTGACCGGAAAGTTGTCCAGAAACATCCCTCTGTTAATCGTCTAGCTTGGACGATAGGAA +ATCGAGCACCGTATATTGGCTCACGGACAGAAGATAAGATCGGTTATCCTCCCTTAAGAG +TAAATTGCCCATCAGCAGCACTTAAAGAAGCTATTGAGATGGTTTCTAGATTGTTATGGG +TGACTCAAGGCACTGCAGACCGAGAAAAATTGCTTATTCCTCTTCTCAATTCAAGAGTAA +ATCTGGACTATCAGACGGTGCTTAACTTTTTACCTACACACTACTCAGGCAACATAGTTC +ATAGATATAATGATCAATATGGACAACATTCCTTTATGGCAAACAGGATGAGTAATACAT +CTACACGTGCAATTATATCAACTAACACACTGGGTAAATATGCTGGGGGAGGTCAAGCTG +CTATTGATAGTAATATAATCTTTCAAAATACTATTAATTTAGGAGTTGCAGTTTTAGATA +TTGCATTGTCTCTTGCTAAATTGTCGTCAGCATCAAATGTCACTTTCCGTTTAATGTTAA +ATAAGTGCTGCACGCGGCATGTACCGTCCGAATACCTATATTTTGATAAACCTTTAGATG +TGGATTTGAACAAGTATATGGACAATGAGTTGGTTTATGACAATGACCCTCTTTGCAGTG +GGATTAAAGGGAGATTAGGCAGAGTATCTCGATCAACACTCACATTGAGTTTGAATGTCA +GTGACATTGGTTCTTATGACTTTCCAACTATTGCTGCATGGACACTAGGAGAAACTATAG +TCGGAAGCATTTTTTCTGATGAATCTTCTCAAAGTACGGATCCAATAAGCTCAGGTTGCA +CAAAAACTTTCGTCACACATTTCCTTGTGTATCCAGTTGAGAGTATATTTTATGCATTCG +GAGCTAACTTAATAGTTGAAAGTTTAAGTCTAAGTAGGATCAAATCAATTAAGAACCTCT +CAGATTTGACATTCCTTATATCATCCACAATCAGGAATTTATCACATAGATCACTTCGGA +TTCTTCAATCTACTTTCCGACATGAATTGGTGCTCACCCGACTAGCCCACCACATACCGT +TAATTTCTTTAATGTTAGGGGGCTCTGCAGGAGAGAAGAGTTCATCAGATGCTGTTCGGC +TATTTCTTACAGCAAGTTATCAGAATTTTATCAATAATTTCAGTTGTCTGATGAAGAAGG +GTCAGTCATCGCTACCGGTTTGGCTTTACTTTCCTAGTGAAGGGCAACAATTAAAGCCTA +TATTAAAAATCTTACAGAGATTATCAGACTTGTTATCACCTGACAAAATTCAAAAGCGTA +AAATTTTGGCTGACACCTGTTGTCCAATTGGCAGCTTTTGGGTCTATCCAAGCAAGTCCA +CAAGGACTAACCATTATTATGCAAGCCTTAATTATTGGAGAGACAAAGCTAATAAGGTTA +AGAATACTCCTTTTTCACACTTGATAAATTGTTCATTTCCTGAATTTTCTTCACATACCA +GTTCAGTCTCCTCTAATCAACAAGTGACCAATTCGAAGTATATTGTTTATCCAGAAAATA +TCACTGAAATAAATGCAAGAACCAGATTAATAAATTATGGATCAACAGCTCTACAGGGGA +TGGACACCAAGATGCCACTCTCAGAGCAAAATCTAGTCGAAAATTGTCGACCATCAGAGG +GCATCAGATTCAAGGACAATCAAAAAATAACAAAACATGACCAGAGATGTGAGAGGGAGG +AATCTTCACCGCAACAGATGTTCCCTGAAGATAACATGCAGACTCCTGCGCACATACATA +GTTCCTCCCCATTTCAAATCCTTATAAAATCACTAGATGCACATGAGGACTTTGATGCCT +CGAAGATAATCTTAAATTCTGAAATAAATAATCTTAACCTTACGGAGTATACTCTTAATA +CAAAGTTATTGACAACTCCTACCAGGACAGAAATTTTAGATACAAGTCCGTTACAATCCT +CTAGATATTCATCAACTTCCAGGGAACGGTCTCTACTATCCAGAGAACAAGCTTCATATT +TGTACGTTGATTGCAGTAATATTCCTTCTATCTCTCTAGACCCAGGTTTTCGGAGTATGT +CTGATCAGAATCAAGTTCAAATGTTAATCAATACCTACAAACGTGATTTACATGCTTGTT +TTGATAGCAATCAATTCTGTCGGTTTACAGGGGTAGTCTCATCAATGCATTACAAGCTTT +ATGATCTTTTGCCTCCAGGTAAATTGAAAAAGGCAATTTGTTTGGCCGAAGGGGAAGGAA +GTGGTGCTCGGTTACTTTTGAAGTGGAAGGAAACGGATTATTTATTCTTCAACACTTTGG +CTACGGATTCACAACAAGAAGCCGAGATTTTGAGTGGCCGGGTAATACCGAGAATGTTGT +ATAACATAGACAGATTAAGTGCTTTGCTTGAATCAAGGAGACTAATATTGAACAACCTAA +CTATCCAAATTACAGATATTACAAATCCATTATGGCTAGATTCTGTAATACAATATTTAC +CTGAAGATAGTGACATTCTTACAATGGACGCAGAGACCACCAAGGATGAAACAAGGGAAC +AGCTTTATAAAACTATTGTGAATATTTGGACACGTACTTCTCCTAATATCCCAAAAATTA +GCATCATCAAGGTATTTTTATTAGACTATGAAGGGACTTTATTCTTAATGAAGAATGCTA +TTCAGTATTATGGGCAAGTTCAACTCAAGAAACCATATAGCTCAAATGCAAAAAACTCAG +AATGGTACTTGTGTTGCGGTAAACGAAGAATTCAACGGCTCCAAATTGATTTCTCAGACC +AGGTGGGAATTTTTCTGATTTGTAAAGCAATGTCACGCCAAAGACAAGCAATTCCTTACT +GGTTAAAACATATAGAAAAGAATTATCCTGCTTCATTACACGAGTTTTTCCTAACTTTGG +GTTTCCCTTCTTTAGAGTCATCTTTCTGCCATCGTTATACTATTCCATTCAGTGAAGGAA +AGGCTCTTTTTCACAAGGTCCAGTCTTATGTTCGTCAAGGCAAACAACATTTACATTCTC +TTATGTTGGATTATGAAAACAATTCACCTCTACTAGACTTGAGAAATCACTTTATTTGCT +CATTAAGGGGAAAGATAACTAAATATTACAATGATATATTAAAGTTAAATCTAGTCATCA +AGGCAGTAGAAAAAGGTAAAAATTGGTCACAACTTGTTGAGATCCTTCCTAATATGCATT +CAGTATGCATAGTGCACGTGGATCATGAGTGTTCTGGATGCGAGAAACGGTTATTACTTA +AATTGGATTTTATCAGAAATACAAAGATCGCAGAACAAAAATTACTTAACAGAGTAATCG +GGTATATCCTATTCTTTCCATTCGGTCTGTTTAAATCTGGATCATTAAGGGCATAATTTC +AACAGAGAGAACTTCATTTAATTCACAAAAACAATCTATTTAAGAGTGAGGGTTACATTG +TCTAAGATATTGTATGAGAAGTAATAAAATAAATAAGAAAACGAAAAGACTATTAGACAG +CTTATTTTATACAAGATAATCTTATATCGCTTTAGGCCTCACACAAGTGAGAAAATTACG +CGCACAGATTAACTAGTGATTAGTGTTTGGTCACACCAGAGGTAACTTTTTAACGTTAAT +TACTCAGATGTTATTGCTCATAATTAGCATTAATATTGGCACATTGGGTGAATCCTTGAG +CTTTATCCCTAATATGGTGTAAGAAATTAAGGAAATACTGAGATACACTAGTTGAATTGA +ATTATGACATACCATATATCATAAATATAAAAAAGTGTCTGCTGTAATCTACAAGCACCT +CTTTTAAATACATTAGGAAAAGAATTAAGTTACCGTTGAGATCAAAAAAACCACGTCATG +TTTTCTCTGATGACAAGTGATAAAACTTCGTAGTTAAATTTCTAGAATGTCGATGTGAAT +GTAAATTAAGAAAAACCAATATATAAAATTAAAAAATTAAAAAGCTTTGATATAAGTAAC +ACAAAACATTCTTCATCTTTTTTGTGTGTCC diff --git a/primerschemes/artic-marv/1200/v1.0.0/work/config.json b/primerschemes/artic-marv/1200/v1.0.0/work/config.json new file mode 100644 index 0000000..f5a99fc --- /dev/null +++ b/primerschemes/artic-marv/1200/v1.0.0/work/config.json @@ -0,0 +1,32 @@ +{ + "amplicon_size": 1200, + "amplicon_size_max": 1380, + "amplicon_size_min": 1020, + "backtrack": false, + "circular": false, + "dimer_score": -29.0, + "force": false, + "high_gc": false, + "ignore_n": true, + "input_bedfile": null, + "mapping": "first", + "min_base_freq": 0.02, + "min_overlap": 100, + "mismatch_kmersize": 19, + "msa_data": { + "0": { + "msa_checksum": "57f830990ffe3ae685d1f6ce5fa2b486", + "msa_chromname": "MARV|NC_001608|Mt._Elgon-Musoke|human|Kenya|Kenya|1980", + "msa_name": "MARV_RAVV_Genbank_2024-09-30.aln3", + "msa_path": "work/MARV_RAVV_Genbank_2024-09-30.aln3.fasta", + "msa_uuid": "2ee06dc7" + } + }, + "n_pools": 2, + "output": "/Users/Josh/bioinfo/marburg_rwanda/artic-marburg-1200-4", + "primer_gc_max": 55, + "primer_gc_min": 30, + "primer_size_max": 34, + "primer_size_min": 19, + "use_matchdb": true +} \ No newline at end of file diff --git a/primerschemes/artic-marv/1200/v1.0.0/work/plot.png b/primerschemes/artic-marv/1200/v1.0.0/work/plot.png new file mode 100644 index 0000000..c2bbf52 Binary files /dev/null and b/primerschemes/artic-marv/1200/v1.0.0/work/plot.png differ