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FileNotFoundError in step 3 #19

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drs opened this issue Oct 28, 2024 · 0 comments
Open

FileNotFoundError in step 3 #19

drs opened this issue Oct 28, 2024 · 0 comments

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@drs
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drs commented Oct 28, 2024

Hello,

I used Broccoli (version 1.3) on a dataset of 160 eukaryotic proteomes.

broccoli.py -steps 1,2,3 -threads 8 -dir Proteomes -path_fasttree /software/apps/core/fasttree/2.1.11/FastTree -phylogenies nj

During the extract para from trees part of step 3 the analysis crashed with the following error. The analysis of the example_dataset from this repository using the same command executed without issues. Do you have any idea what might be causing this issue?

 --- STEP 3: network analysis

 ## parameters
 species overlap  : 0.5
 min edge weight  : 0.1
 min nb hits      : 2
 chimeric edges   : 0.5
 chimeric species : 3
 threads          : 8

 ## get ortho and para
 extract ortho from similarity
 extract ortho from trees
 remove ortho found only once
 extract para from trees
Traceback (most recent call last):
  File "/software/apps/core/broccoli/1.3/broccoli.py", line 153, in <module>
    broccoli_step3.step3_orthology_network(sp_overlap, min_weight, min_nb_hits, chimeric_shared, chimeric_nb_sp, nb_threads)
  File "/software/apps/core/broccoli/1.3/scripts/broccoli_step3.py", line 78, in step3_orthology_network
    extract_para(files_trees)
  File "/software/apps/core/broccoli/1.3/scripts/broccoli_step3.py", line 301, in extract_para
    content_pickle = pickle.load(open(path_tmp_para / filename, 'rb'))
FileNotFoundError: [Errno 2] No such file or directory: 'dir_step3/tmp_para/0_trees.pic'

Best,
Samuel

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