diff --git a/data/8_color_data_set/8_color_ICS_boolean_gate_testing.wsp b/data/8_color_data_set/8_color_ICS_boolean_gate_testing.wsp
new file mode 100644
index 00000000..2ac30f67
--- /dev/null
+++ b/data/8_color_data_set/8_color_ICS_boolean_gate_testing.wsp
@@ -0,0 +1,3935 @@
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+                               <NotNode name="TNFa+-"  annotation=""  owningGroup="DEN"  expanded="1"  sortPriority="13"  count="46290" >
+                                 <Graph smoothing="0"  backColor="#fefefe"  foreColor="#000000"  heatMapStatParameter="Comp-TNFa FITC FLR-A"  type="Pseudocolor"  fast="1" >
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+                                   <Axis dimension="y"  name="Comp-TNFa FITC FLR-A"  label=""  auto="auto" />
+                                   <GraphSettings level="5%"  smoothingHighResolution="1"  contourHighResolution="1"  histogramSmoothingCount="0"  graphResolution="256"  showOutliers="0"  drawLargeDots="0"  dotsToDraw="8000" />
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+                                     <TextTraits font="SansSerif"  size="9"  name="Numbers"  style="plain"  color="#000000"  background="#00ffffff"  just="left" />
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+                                     <Graph smoothing="0"  backColor="#fefefe"  foreColor="#000000"  heatMapStatParameter="Comp-TNFa FITC FLR-A"  type="Pseudocolor"  fast="1" >
+                                       <Axis dimension="x"  name="Comp-CD3 APC-H7 FLR-A"  label=""  auto="auto" />
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+                                         <TextTraits font="SansSerif"  size="11"  name="LayoutGates"  style="plain"  color="#000000"  background="#00ffffff"  just="left" />
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+                                 <Dependents>
+                                   <Dependent name="Time/Singlets/aAmine-/CD3+/CD8+/TNFa+" />
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+       <transforms:spilloverMatrix spectral="0"  prefix="Comp-"  name="Acquisition-defined"  editable="0"  color="#c0c0c0"  version="FlowJo-10.8.1"  status="FINALIZED"  transforms:id="99dfa639-6268-4a59-8cf8-d0b01c7a6b21"  suffix="" >
+         <data-type:parameters>
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+           <data-type:parameter data-type:name="CD8 PerCP-Cy55 FLR-A"  userProvidedCompInfix="Comp-CD8 PerCP-Cy55 FLR-A" />
+           <data-type:parameter data-type:name="IL2 BV421 FLR-A"  userProvidedCompInfix="Comp-IL2 BV421 FLR-A" />
+           <data-type:parameter data-type:name="Aqua Amine FLR-A"  userProvidedCompInfix="Comp-Aqua Amine FLR-A" />
+           <data-type:parameter data-type:name="IFNg APC FLR-A"  userProvidedCompInfix="Comp-IFNg APC FLR-A" />
+           <data-type:parameter data-type:name="CD3 APC-H7 FLR-A"  userProvidedCompInfix="Comp-CD3 APC-H7 FLR-A" />
+           <data-type:parameter data-type:name="CD107a PE FLR-A"  userProvidedCompInfix="Comp-CD107a PE FLR-A" />
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diff --git a/src/flowkit/_utils/wsp_utils.py b/src/flowkit/_utils/wsp_utils.py
index f8ce7fe1..9d1d86d5 100644
--- a/src/flowkit/_utils/wsp_utils.py
+++ b/src/flowkit/_utils/wsp_utils.py
@@ -16,7 +16,7 @@
 # noinspection PyProtectedMember
 from .._models.transforms import _transforms, _wsp_transforms
 # noinspection PyProtectedMember
-from .._models.gates._gates import PolygonGate, RectangleGate
+from .._models.gates._gates import PolygonGate, RectangleGate, BooleanGate
 # noinspection PyProtectedMember
 from .._models.gates._gml_gates import \
     GMLBooleanGate, \
@@ -232,6 +232,109 @@ def _parse_wsp_keywords(keywords_el):
 
     return keywords_lut
 
+def _parse_population_node(pop_el, parent_id, gating_ns, data_type_ns):
+    """
+    Given a Population node, return a gate instance
+
+    :param pop_el: Population element
+    :param parent_id: parent gate ID as list
+    :param gating_ns: GatingML gating namespace
+    :param data_type_ns: GatingML data type namespace
+    :return: gate instance
+    """
+    ns_map = pop_el.nsmap
+    pop_name = pop_el.attrib['name']
+    gate_el = pop_el.find('Gate', ns_map)
+
+    gate_child_els = gate_el.getchildren()
+
+    if len(gate_child_els) != 1:
+        raise ValueError("Gate element must have only 1 child element")
+
+    gate_child_el = gate_child_els[0]
+
+    # determine gate type
+    # TODO: this string parsing seems fragile, may need to be shored up
+    gate_type = gate_child_el.tag.partition('}')[-1]
+    gate_class = wsp_gate_constructor_lut[gate_type]
+
+    # Lookup 'eventsInside' attribute value, determines whether gate results
+    # should within the gate area or outside (i.e. the gate's complement).
+    # The value is a text string of an integer: '1' for events inside,
+    # '0' for events outside.
+    try:
+        events_inside_value = int(gate_child_el.attrib['eventsInside'])
+    except KeyError:
+        # if not found, default to events inside gate
+        events_inside_value = 1
+
+    if events_inside_value == 1:
+        use_complement = False
+    else:
+        use_complement = True
+
+    if gate_type in ['RectangleGate', 'PolygonGate', 'EllipsoidGate']:
+        # these take extra kwarg 'use_complement'
+        g = gate_class(
+            gate_child_el,
+            gating_ns,
+            data_type_ns,
+            use_complement=use_complement
+        )
+    else:
+        g = gate_class(
+            gate_child_el,
+            gating_ns,
+            data_type_ns
+        )
+
+    # FlowJo gates don't store gate names quite the same as GatingML.
+    # Tag the 'gate_name' & 'parent' attributes so we can re-create
+    # the correct hierarchy later.
+    g.gate_name = pop_name
+    g.parent = parent_id
+
+    return g
+
+def _parse_boolean_node(bool_el, bool_gate_type, parent_id):
+    """
+    Given a Boolean node, return a gate instance
+
+    :param bool_el: Boolean node element (AndNode, OrNode, NotNode)
+    :param bool_gate_type: Boolean type: 'and', 'or', or 'not'
+    :param parent_id: parent gate ID as list
+    :return: gate instance
+    """
+    gate_name = bool_el.attrib['name']
+    deps_el = bool_el.find('Dependents')
+
+    # There should be one 'Dependents' node.
+    # Within there may be one or more 'Dependent' nodes
+    dep_els = deps_el.findall('Dependent')
+
+    # create gate ref dicts needed for BooleanGate constructor
+    gate_refs = []
+    for dep_el in dep_els:
+        # each gate ref is a dict containing:
+        #  'ref': reference gate name
+        #  'path': tuple of reference gate path
+        #  'complement': Boolean value for whether to use gate complement
+        dep_name = dep_el.attrib['name']
+
+        # dependent name is a string w/ '/' separators
+        # but doesn't have FlowKit's 'root' base, so we need to add it
+        ref_gate_id = dep_name.split('/')
+        ref_gate_id.insert(0, 'root')
+        gate_ref = {
+            'ref': ref_gate_id[-1],
+            'path': tuple(ref_gate_id[:-1]),
+            'complement': False  # FlowJo doesn't seem to have this option
+        }
+        gate_refs.append(gate_ref)
+
+    g = BooleanGate(gate_name, bool_gate_type, gate_refs)
+
+    return g
 
 def _convert_wsp_gate(wsp_gate, comp_matrix, xform_lut):
     new_dims = []
@@ -306,6 +409,9 @@ def _convert_wsp_gate(wsp_gate, comp_matrix, xform_lut):
         # ellipses to polygons internally when processing gates.
         gate = wsp_gate.convert_to_polygon_gate(xforms)
         gate.dimensions = new_dims
+    elif isinstance(wsp_gate, BooleanGate):
+        # no need to convert BooleanGate instances
+        gate = wsp_gate
     else:
         raise NotImplemented(
             "%s gates for FlowJo workspaces are not currently supported." % type(wsp_gate).__name__
@@ -322,68 +428,27 @@ def _recurse_wsp_sub_populations(sub_pop_el, gate_path, gating_ns, data_type_ns)
         # here we'll create the gate path as a list b/c we will append to it for recursion
         # however, when it is finally stored in the list of gate dicts we will convert to tuple
         gate_path = ['root']
-        parent_id = None
+        parent_gate_name = None
     else:
-        parent_id = gate_path[-1]
+        parent_gate_name = gate_path[-1]
 
+    # regular gate (rectangle, polygon, etc.) are within 'Population' nodes
     pop_els = sub_pop_el.findall('Population', ns_map)
-    for pop_el in pop_els:
-        pop_name = pop_el.attrib['name']
-        owning_group = pop_el.attrib['owningGroup']
-        gate_el = pop_el.find('Gate', ns_map)
-
-        gate_child_els = gate_el.getchildren()
 
-        if len(gate_child_els) != 1:
-            raise ValueError("Gate element must have only 1 child element")
+    # Boolean gates are not in 'Population' nodes & have their own dedicated
+    # node. And each type has a different tag so we need to find them separately.
+    boolean_node_lut = {}
+    boolean_node_lut['and'] = sub_pop_el.findall('AndNode', ns_map)
+    boolean_node_lut['or'] =  sub_pop_el.findall('OrNode', ns_map)
+    boolean_node_lut['not'] = sub_pop_el.findall('NotNode', ns_map)
 
-        gate_child_el = gate_child_els[0]
-
-        # determine gate type
-        # TODO: this string parsing seems fragile, may need to be shored up
-        gate_type = gate_child_el.tag.partition('}')[-1]
-        gate_class = wsp_gate_constructor_lut[gate_type]
-
-        # Lookup 'eventsInside' attribute value, determines whether gate results
-        # should within the gate area or outside (i.e. the gate's complement).
-        # The value is a text string of an integer: '1' for events inside,
-        # '0' for events outside.
-        try:
-            events_inside_value = int(gate_child_el.attrib['eventsInside'])
-        except KeyError:
-            # if not found, default to events inside gate
-            events_inside_value = 1
-
-        if events_inside_value == 1:
-            use_complement = False
-        else:
-            use_complement = True
-
-        if gate_type in ['RectangleGate', 'PolygonGate', 'EllipsoidGate']:
-            # these take extra kwarg 'use_complement'
-            g = gate_class(
-                gate_child_el,
-                gating_ns,
-                data_type_ns,
-                use_complement=use_complement
-            )
-        else:
-            g = gate_class(
-                gate_child_el,
-                gating_ns,
-                data_type_ns
-            )
+    # recurse over 'Population' nodes
+    for pop_el in pop_els:
+        g = _parse_population_node(pop_el, parent_gate_name, gating_ns, data_type_ns)
+        owning_group = pop_el.attrib['owningGroup']
 
-        # replace ID and parent ID with population names, but save the originals,
-        # so we can re-create the correct hierarchy later.
-        # NOTE: Some wsp files have the ID as an attribute of the GatingML-style sub-element,
-        #       though it isn't always the case. However, it seems the ID is reliably included
-        #       in the parent "Gate" element, saved here in the 'gate_el' variable.
-        #       Likewise for parent_id
-        g.gate_name = pop_name
-        g.parent = parent_id
         gate_id = copy.copy(gate_path)
-        gate_id.append(pop_name)
+        gate_id.append(g.gate_name)
         gate_id = tuple(gate_id)
 
         # Including gate path for ease of access later, even though a bit redundant w/ gate_id
@@ -394,10 +459,32 @@ def _recurse_wsp_sub_populations(sub_pop_el, gate_path, gating_ns, data_type_ns)
         }
 
         sub_pop_els = pop_el.findall('Subpopulations', ns_map)
-        child_gate_path = copy.copy(gate_path)
-        child_gate_path.append(pop_name)
+
         for el in sub_pop_els:
-            gates.update(_recurse_wsp_sub_populations(el, child_gate_path, gating_ns, data_type_ns))
+            gates.update(_recurse_wsp_sub_populations(el, list(gate_id), gating_ns, data_type_ns))
+
+    # and now recurse over the various Boolean nodes
+    for bool_type, bool_type_nodes in boolean_node_lut.items():
+        for bool_el in bool_type_nodes:
+            g = _parse_boolean_node(bool_el, bool_type, parent_gate_name)
+            owning_group = bool_el.attrib['owningGroup']
+
+            gate_id = copy.copy(gate_path)
+            gate_id.append(g.gate_name)
+            gate_id = tuple(gate_id)
+
+            # Including gate path for ease of access later, even though a bit redundant w/ gate_id
+            gates[gate_id] = {
+                'owning_group': owning_group,
+                'gate': g,
+                'gate_path': tuple(gate_path)  # converting to tuple!
+            }
+
+            # Like Population nodes, the Boolean nodes can contain sub-pops
+            sub_pop_els = bool_el.findall('Subpopulations', ns_map)
+
+            for el in sub_pop_els:
+                gates.update(_recurse_wsp_sub_populations(el, list(gate_id), gating_ns, data_type_ns))
 
     return gates
 
diff --git a/tests/workspace_tests.py b/tests/workspace_tests.py
index 3f68f497..7d6b52fb 100644
--- a/tests/workspace_tests.py
+++ b/tests/workspace_tests.py
@@ -10,6 +10,7 @@
 from flowkit import Workspace, Sample, Matrix, gates, transforms, extract_wsp_sample_data
 # noinspection PyProtectedMember
 from flowkit._models.transforms._base_transform import Transform
+from flowkit._models.gating_results import GatingResults
 from flowkit.exceptions import GateReferenceError
 
 from tests.test_config import test_samples_8c_full_set
@@ -425,6 +426,55 @@ def test_parse_wsp_with_ellipse(self):
         self.assertIsInstance(gate_indices, np.ndarray)
         self.assertEqual(np.sum(gate_indices), 7023)
 
+    def test_parse_wsp_with_boolean_gates(self):
+        wsp_path = "data/8_color_data_set/8_color_ICS_boolean_gate_testing.wsp"
+        fcs_path = "data/8_color_data_set/fcs_files/101_DEN084Y5_15_E01_008_clean.fcs"
+        sample_id = '101_DEN084Y5_15_E01_008_clean.fcs'
+
+        # Boolean AND gate in CD4+ branch
+        gate_name_01 = 'CD107a+ & IFNg+'
+        gate_path_01 = ('root', 'Time', 'Singlets', 'aAmine-', 'CD3+', 'CD4+')
+
+        # Boolean OR gate in CD4+ branch
+        gate_name_02 = 'CD107a+ or IFNg+'
+        gate_path_02 = ('root', 'Time', 'Singlets', 'aAmine-', 'CD3+', 'CD4+')
+
+        # Boolean NOT gate in CD4+ branch
+        gate_name_03 = 'IL2+-'
+        gate_path_03 = ('root', 'Time', 'Singlets', 'aAmine-', 'CD3+', 'CD4+')
+
+        # Boolean NOT gate in CD4+ branch
+        gate_name_04 = 'TNFa+-'
+        gate_path_04 = ('root', 'Time', 'Singlets', 'aAmine-', 'CD3+', 'CD4+')
+
+        # Boolean NOT gate in CD8+ branch
+        gate_name_05 = 'TNFa+-'
+        gate_path_05 = ('root', 'Time', 'Singlets', 'aAmine-', 'CD3+', 'CD8+')
+
+        # Child of a Boolean gate in CD8+ branch
+        gate_name_06 = 'CD107a+'
+        gate_path_06 = ('root', 'Time', 'Singlets', 'aAmine-', 'CD3+', 'CD8+', 'TNFa+-')
+
+        wsp = Workspace(wsp_path, fcs_samples=fcs_path, ignore_missing_files=True)
+
+        wsp.analyze_samples(sample_id=sample_id)
+        gating_results = wsp.get_gating_results(sample_id)
+
+        gate_count_01 = gating_results.get_gate_count(gate_name_01, gate_path_01)
+        gate_count_02 = gating_results.get_gate_count(gate_name_02, gate_path_02)
+        gate_count_03 = gating_results.get_gate_count(gate_name_03, gate_path_03)
+        gate_count_04 = gating_results.get_gate_count(gate_name_04, gate_path_04)
+        gate_count_05 = gating_results.get_gate_count(gate_name_05, gate_path_05)
+        gate_count_06 = gating_results.get_gate_count(gate_name_06, gate_path_06)
+
+        self.assertIsInstance(gating_results, GatingResults)
+        self.assertEqual(gate_count_01, 0)
+        self.assertEqual(gate_count_02, 72)
+        self.assertEqual(gate_count_03, 82478)
+        self.assertEqual(gate_count_04, 82463)
+        self.assertEqual(gate_count_05, 47157)
+        self.assertEqual(gate_count_06, 70)
+
     def test_get_ambiguous_gate_objects(self):
         wsp_path = "data/8_color_data_set/8_color_ICS.wsp"
         fcs_path = "data/8_color_data_set/fcs_files/101_DEN084Y5_15_E01_008_clean.fcs"