How to ignore filenames

[josh-gree]

Hi - thanks for creating this amazing tool!

I would love to understand if its possible to completely ignore anything about sample filenames and ids and the like - I just want to be able to take the gating tree from some wsp and apply it arbitrarily to a given sample - it may well be the case that the gating tree and the sample don't work together but I would be validating that separately and externally from flowkit - I am sure the machinery exists to do this within FlowKit but not quite sure how to get to it?

[whitews]

Hi Josh,

Great to hear you find the library useful.

Yes, there is a way to do this. Once you load a FlowJo .wsp file as Workspace instance, use the get_gating_strategy method to retrieve a GatingStrategy instance. That method takes a sample_id argument to get the specific gating strategy for that sample. Note, if you don't have the FCS sample, you can load the .wsp file using ignore_fcs_files=True and the workspace will still load the gating information so you can retrieve it. Then, the GatingStrategy instance can be used against any sample using its gate_sample method.

The GatingStrategy class is the engine behind both the Session and Workspace classes. However, it was also made to be used for such a scenario where you don't want the library to manage samples for you.

Hope this helps and let me know if that's what you were looking for.

Kind regards,
Scott