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Introduction to scRNA-seq Using R

Preparing students to apply for a research position in genomic data science [slides]

Start your genomics adventure at Clovis Community College this Fall 2021

Assumes high school biology and algebra, but no programming experience

Overview of Modules

See Sample Schedule for additional details

  • Data Exploration: Researching genes using biological databases
    • What Makes Cells Different? [slides]
    • Spreadsheet of Gene Expression [slides]
    • Biological Databases (Assignment / Discussion) [assignment]
    • Data Exploration Project (Start / Finish / Presentation) [assignment]
  • Single Cell: Increasing genomic resolution with new technologies
    • What Does Single Cell Enable?
    • cellxgene for scRNA-seq (Assignment / Discussion) [slides]
    • Single Cell Project (Start / Finish / Present)
  • Pathway Analysis: Integrating genes using molecular networks
    • How Do Genes Work Together?
    • KEGG for Pathways (Assignment / Discussion) [slides]
    • Pathway Analysis Project (Start / Finish / Present)
  • Genes and Genomes: Understanding the role of genome organization
    • How Are Genes Regulated?
    • Genome Browser (Assignment / Discussion)
    • Genes and Genomes Project (Start / Finish / Present)
  • Scientific Literature
    • How to Read a Paper
    • Figure Analysis [template]
    • Reproducing Individual Results
  • Project Work
    • Generating Hypotheses
    • Peer Review
    • How to Make a Poster
  • Student Professional Development
    • Apply for Next Research Experience
    • Poster Symposium
    • Web Portfolio

Interactive LearnR Tutorials

Preview some of our tutorials at cure-rnaseq/tutorials

Note: Rendered previews are provided where possible, but tutorials that involve larger datasets or require more compute may not be available. Tutorials run in most Shiny environments (e.g. local RStudio, sciserver.org, mybinder.org, shinyapps.io).

Sample Schedule

Day 1

In Class

  • Lecture: Welcome to Your Genomics Adventure! [slides]
  • Lecture: The Scientific Process [slides]
  • Activity: Create Accounts

Homework

  • Assignment: Post to Discourse Forums [assignment]

Day 2

In Class

Homework

Day 3

In Class

Homework

Day 4

In Class

Homework

  • Assignment: Swirl -- The basics of programming in R (3,4,5,6,8,12)
  • Assignment: RStudio Console
  • Assignment: cellxgene for scRNA-seq [slides]

Day 5

In Class

  • Discussion: cellxgene for scRNA-seq [slides]
  • Assignment: Single Cell Project (Start)
  • Activity: Managing Data in the Cloud

Homework

  • Assignment: R for Data Science (Introduction)
  • Assignment: Single Cell Project (Finish)

Day 6

In Class

  • Presentation: Single Cell Project
  • Lecture: How Do Genes Work Together?
  • Assignment: Working with R Notebooks

Homework

Student Professional Development

Web Portfolios Using GitHub [repo][demo]

Getting Help

Join the conversation in our Slack or Discourse forums!


C-MOOR is supported in part by Carnegie Science Venture Grant and NSF DUE Award #2021013

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