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Nucleic Acid extraction kits
---
title: DNA and RNA kits by sample
category: Experimental-Procedure-Standards-SOPs
layout: default
docs_css: markdown
---

DNA extraction
Sponges - QIAamp® DNA Micro kit (QIAGEN)1
Sponges –DNeasy® PowerSoil® Pro Kit (QIAGEN)1
Human blood viruses - QIAAMP Ultra Sens Virus Kit (Qiagen)2
Water samples - PowerSoil® DNA Isolation Kit (MoBio)3
Saliva - QIAamp® DNA Mini kit (Qiagen)4
Crushed fruit fly larvae - DNeasy PowerLyzer PowerSoil Kit-100 (Qiagen)5
Frog skin swab – Prepman Ultra (ThermoFisher)6
Skin swabs - PureLink® Genomic DNA Mini Kit (Life Technologies)7
Skin swaps - Kaneka Easy DNA Extraction Kit version 2 (Kaneka Corp)8
Oral swaps - QIAamp DNA mini kit (Qiagen)9
Skin swaps - QIAamp blood and tissue DNA extraction kit (Qiagen)9
Oyster haemolymph - Qiagen DNeasy Blood and Tissue Kit (Qiagen)10
Molluscs - E.Z.N.A. Mollusc DNA (Omega Biotek)11
Melted ice cave water - MoBio PowerWater DNA Isolation kit (MoBio)12
BAL, oral rinse, tongue scraping, bronchoscope flush - DNeasy Blood and Tissue kit (Qiagen)13
Lake sediment - PrestoTM Soil DNA Extraction Kit14
Oral Swabs - Maxwell RSC PureFood Pathogen Kit (Promega)15
Plant DNA - Mo Bio PowerSoil kit (Qiagen)16
Gastric juice - DNeasy® PowerSoil Pro kit (Qiagen)17
Saliva - DNeasy® PowerSoil Pro kit (Qiagen)18
Bacterial DNA - DNeasy PowerSoil Kit (QIAGEN)19
Soil - PowerSoil DNA Isolation Kit (Qiagen)20
Soil - FastDNA Spin kit for soil (MP Bio)21
Wastewater samples - Nucleo spin soil DNA kit (Macherey Nagel)22
Saliva - OMNIgene ORAL OM-501 (Genotek)23
Viral DNA - QIAGEN DNeasy Blood and Tissue Kit (Qiagen)24
Viral DNA & RNA - AllPrep DNA/RNA Mini kit (Qiagen)25
Microbial DNA - Mo Bio PowerSoil kit (Qiagen)25
Phage DNA - QIAamp - DNA stool kit (Qiagen)26
Bacterial & Viral DNA - Isolate II Genomic DNA Kit (Bioline)13
Fungal DNA - Maxwell® RSC PureFood GMO and Authentication Kit (Promega)27
Leaves - PureLink Genomic DNA (ThermoFisher)28
Food – Power Food kit (Qiagen)33
Mollusc tissues - Qiagen DNeasy Blood and Tissue Kit (Qiagen)34 JV
Yogurt - PowerSoil DNA Isolation Kit (MoBio)35 Pamela
Saliva - PowerSoil DNA Isolation Kit (MoBio)36, Pamela
Plasmid preparations: NucleoSpin Plasmid kit (Macherey-Nagel, Düren, DE)37 Maja
Bile samples - ZymoBIOMICS DNA/RNA Miniprep Kit38 NT
mouse small intestine content - ZymoBIOMICS DNA/RNA Miniprep Kit39 NT
Bacterial DNA from cultures (automated) - GenFind v3 Kit (Beckman Coulter)40 NT
Bacterial DNA from low biomass (eg. FACS sorted) samlpes – mericon DNA Bacteria Kit (Qiagen)41 NT

RNA extraction
Mosquitos - TRIzol LS (Invitrogen) – purified with RNeasy MinElute Cleanup Kit (Qiagen)29
Sea Water - RNeasy mini kit (Qiagen)3
Antarctic ticks - MagMax mirVana™ Total RNA isolation Kit (ThermoFisher Scientific)30
Soil - PowerMax soil DNA isolation kit (Mo Bio)31
Cervical samples - Smarter stranded total RNA-seq kit v2—pico input mammalian (Takara Bio)32
Viral DNA & RNA - AllPrep DNA/RNA Mini kit (Qiagen)25
Viral RNA - QIAmp Viral RNA kit (Qiagen)13
Cerebellum, hippocampus and visual cortex - Uneasy Plus Universal Mini Kit (Qiagen)42 JV
Bile samples - ZymoBIOMICS DNA/RNA Miniprep Kit43 NT
mouse small intestine content - ZymoBIOMICS DNA/RNA Miniprep Kit44 NT
# Nucleic Acid extraction kits

## DNA extraction

References
- Sponges - QIAamp® DNA Micro kit (QIAGEN)1
- Sponges –DNeasy® PowerSoil® Pro Kit (QIAGEN)1
- Human blood viruses - QIAAMP Ultra Sens Virus Kit (Qiagen)2
- Water samples - PowerSoil® DNA Isolation Kit (MoBio)3
- Saliva - QIAamp® DNA Mini kit (Qiagen)4
- Crushed fruit fly larvae - DNeasy PowerLyzer PowerSoil Kit-100 (Qiagen)5
- Frog skin swab – Prepman Ultra (ThermoFisher)6
- Skin swabs - PureLink® Genomic DNA Mini Kit (Life Technologies)7
- Skin swaps - Kaneka Easy DNA Extraction Kit version 2 (Kaneka Corp)8
- Oral swaps - QIAamp DNA mini kit (Qiagen)9
- Skin swaps - QIAamp blood and tissue DNA extraction kit (Qiagen)9
- Oyster haemolymph - Qiagen DNeasy Blood and Tissue Kit (Qiagen)10
- Molluscs - E.Z.N.A. Mollusc DNA (Omega Biotek)11
- Melted ice cave water - MoBio PowerWater DNA Isolation kit (MoBio)12
- BAL, oral rinse, tongue scraping, bronchoscope flush - DNeasy Blood and Tissue kit (Qiagen)13
- Lake sediment - PrestoTM Soil DNA Extraction Kit14
- Oral Swabs - Maxwell RSC PureFood Pathogen Kit (Promega)15
- Plant DNA - Mo Bio PowerSoil kit (Qiagen)16
- Gastric juice - DNeasy® PowerSoil Pro kit (Qiagen)17
- Saliva - DNeasy® PowerSoil Pro kit (Qiagen)18
- Bacterial DNA - DNeasy PowerSoil Kit (QIAGEN)19
- Soil - PowerSoil DNA Isolation Kit (Qiagen)20
- Soil - FastDNA Spin kit for soil (MP Bio)21
- Wastewater samples - Nucleo spin soil DNA kit (Macherey Nagel)22
- Saliva - OMNIgene ORAL OM-501 (Genotek)23
- Viral DNA - QIAGEN DNeasy Blood and Tissue Kit (Qiagen)24
- Viral DNA & RNA - AllPrep DNA/RNA Mini kit (Qiagen)25
- Microbial DNA - Mo Bio PowerSoil kit (Qiagen)25
- Phage DNA - QIAamp - DNA stool kit (Qiagen)26
- Bacterial & Viral DNA - Isolate II Genomic DNA Kit (Bioline)13
- Fungal DNA - Maxwell® RSC PureFood GMO and Authentication Kit (Promega)27
- Leaves - PureLink Genomic DNA (ThermoFisher)28
- Food – Power Food kit (Qiagen)33
- Mollusc tissues - Qiagen DNeasy Blood and Tissue Kit (Qiagen)34 JV
- Yogurt - PowerSoil DNA Isolation Kit (MoBio)35 Pamela
- Saliva - PowerSoil DNA Isolation Kit (MoBio)36, Pamela
- Plasmid preparations: NucleoSpin Plasmid kit (Macherey-Nagel, Düren, DE)37 Maja
- Bile samples - ZymoBIOMICS DNA/RNA Miniprep Kit38 NT
- mouse small intestine content - ZymoBIOMICS DNA/RNA Miniprep Kit39 NT
- Bacterial DNA from cultures (automated) - GenFind v3 Kit (Beckman Coulter)40 NT
- Bacterial DNA from low biomass (eg. FACS sorted) samlpes – mericon DNA Bacteria Kit (Qiagen)41 NT

## RNA extraction
- Mosquitos - TRIzol LS (Invitrogen) – purified with RNeasy MinElute Cleanup Kit (Qiagen)29
- Sea Water - RNeasy mini kit (Qiagen)3
- Antarctic ticks - MagMax mirVana™ Total RNA isolation Kit (ThermoFisher Scientific)30
- Soil - PowerMax soil DNA isolation kit (Mo Bio)31
- Cervical samples - Smarter stranded total RNA-seq kit v2—pico input mammalian (Takara Bio)32
- Viral DNA & RNA - AllPrep DNA/RNA Mini kit (Qiagen)25
- Viral RNA - QIAmp Viral RNA kit (Qiagen)13
- Cerebellum, hippocampus and visual cortex - Uneasy Plus Universal Mini Kit (Qiagen)42 JV
- Bile samples - ZymoBIOMICS DNA/RNA Miniprep Kit43 NT
- mouse small intestine content - ZymoBIOMICS DNA/RNA Miniprep Kit44 NT


##References
1. Ruocco, N. et al. Microbial diversity in Mediterranean sponges as revealed by metataxonomic analysis. Scientific Reports 2021 11:1 11, 1–12 (2021).
2. Cebriá-Mendoza, M. et al. Deep viral blood metagenomics reveals extensive anellovirus diversity in healthy humans. Scientific Reports 2021 11:1 11, 1–11 (2021).
3. Martínez-Pérez, C. et al. Phylogenetically and functionally diverse microorganisms reside under the Ross Ice Shelf. Nature Communications 2022 13:1 13, 1–15 (2022).
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---
title: Lipid and fatty acid extraction protocol from biological samples
category: Experimental-Procedure-Standards-SOPs
layout: default
docs_css: markdown
---

Protocol/MCF/SamplePrep/02: Lipid and fatty acid extraction protocol from biological samples

Aim: Lipid extraction from mammalian cells or microbial samples for LC-MS analysis
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Protocol/MCF/SamplePrep/01: Metabolite extraction from adherent mammalian cells
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title: Metabolite extraction from adherent mammalian cells
category: Experimental-Procedure-Standards-SOPs
layout: default
docs_css: markdown
---

Protocol/MCF/SamplePrep/01: Metabolite extraction from adherent mammalian cells

Aim: Aqueous metabolite extraction from mammalian cells or microbial samples for LC-MS analysis

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---
title: Metabolite extraction from plant tissue
category: Experimental-Procedure-Standards-SOPs
layout: default
docs_css: markdown
---


Protocol/MCF/SamplePrep/03: Metabolite extraction from plant tissues

Sample required:Plant material required: 20-50 mg finely homogenized tissue
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---
title: Proteomics TMT labelled SP3 method
category: Experimental-Procedure-Standards-SOPs
layout: default
docs_css: markdown
---


TMT-labelled SP3 method

Buffers:
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---
title: Sample Collection and Storage by Sample
category: Experimental-Procedure-Standards-SOPs
layout: default
docs_css: markdown
---

General Protein Storage1
For short-term storage (~24h), most proteins can be kept at 4°C. For long-term storage, protein samples are typically kept at -20°C or -80°C.
Protein storage at -20°C usually requires the addition of 50% glycerol to your sample to avoid freezing at this temperature. If we plan to store a protein at -20°C, we generally run the final size exclusion chromatography step in 2x storage buffer and then dilute the sample 1:1 with 100% glycerol. Alternatively, the protein sample can also be dialysed against the storage buffer already containing 50% glycerol. Proteins stored at -20ºC are often stable for several months, although the exact time frame is protein-dependent and should be determined experimentally.
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