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fix Acquisition protocol
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EnesSefaAyar committed Apr 17, 2024
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10 changes: 5 additions & 5 deletions R/petrosius2023_mES.R
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##' addition of 1 microL 1% (v/v) trifluoroacetic acid (TFA). All liquid
##' dispensing was done using an I-DOT One instrument.
##' - **Liquid chromatography**: For the HRMS1-DIA experiments and the DIA
##' isolation window survey, the Evosep One liquid chromatography was used.
##' The standard 31 min or 58min pre-defined Whisper gradients were used with a
##' flow rate of 100 nl/min for peptide elution.
##' isolation window survey, Evosep One liquid chromatography was used.
##' The standard 31-minute or 58-minute pre-defined Whisper gradients were
##' used with a flow rate of 100 nl/min for peptide elution.
##' - **Mass spectrometry**: The mass spectrometer was operated in positive
##' mode with the FAIMSPro interface compensation voltage set to -45 V.
##' MS1 scans were carried out at 120,000 resolution with an automatic gain
##' control (AGC) of 300% and maximum injection time set to auto. For the DIA
##' isolation window survey a scan range of 500–900 was used and 400–1000
##' isolation window survey, a scan range of 500–900 was used, and 400–1000
##' rest of the experiments. Higher energy collisional dissociation (HCD) was
##' used for precursor fragmentation with a normalized collision energy (NCE)
##' of 33% and MS2 scan AGC target was set to 1000%.
##' of 33% and the MS2 scan AGC target was set to 1000%.
##' - **Raw data processing**: The mESC raw data files were processed with
##' Spectronaut 17.
##'
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