Repoer error when run plot_pseudotime_heatmap in monocle2, call for help.

[JWDu0122]

cds = readRDS("./cds_heatamp.rds")
Time_diff = read.csv("./20240923/3/Time_diff_all.csv")
Time_genes <- Time_diff$gene_short_name[order(Time_diff$qval)][1:1000]
p5 = plot_pseudotime_heatmap(cds[Time_genes,], num_clusters=5, show_rownames=F, return_heatmap=T)
simpleError in checkwz(wz, M = M, trace = trace, wzepsilon = control$wzepsilon): NAs found in the working weights variable 'wz'>
errori n if (x@family@vfamily %in% c("negbinomial", "negbinomial.size")) {:
the condition has length > 1

sessionInfo()
R version 4.4.1 (2024-06-14 ucrt)
Platform: x86_64-w64-mingw32/x64
Running under: Windows 10 x64 (build 19045)

Matrix products: default

locale:
[1] LC_COLLATE=Chinese (Simplified)_China.utf8 LC_CTYPE=Chinese (Simplified)_China.utf8
[3] LC_MONETARY=Chinese (Simplified)_China.utf8 LC_NUMERIC=C
[5] LC_TIME=Chinese (Simplified)_China.utf8

time zone: Asia/Shanghai
tzcode source: internal

attached base packages:
[1] splines stats4 stats graphics grDevices utils datasets methods base

other attached packages:
[1] monocle_2.33.0 DDRTree_0.1.5 irlba_2.3.5.1 VGAM_1.1-12 ggplot2_3.5.1
[6] Biobase_2.60.0 BiocGenerics_0.51.2 Matrix_1.7-0

[ExpertS04p]

I have the same problem...

[JWDu0122]

I have the same problem...

you can try select top50 gene rerun