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dangling ends and supplementary alignments

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@fidelram fidelram released this 27 Nov 11:25
· 2075 commits to master since this release
  • Thanks to Aleksander Jankowski for fixing the handling of chimeric alignments in hicBuildMatrix (PR #151). Now, for reads with supplementary alignments, only the alignment closer to the 5' end
    of the read is used.

  • We discovered that the handling of dangling ends was too strict as all reads starting with the dangling sequence were considered dangling ends (#152). However, a read frequently could start with the dangling sequence but otherwise have a mate from a proper Hi-C re-ligation. Thus, we now only count reads as 'dangling ends' if the mates are within the maximum library insert size distance, face inwards and one of them starts with the dangling sequence.

  • As part of this release we also improved the help message of hicBuildMatrix. For this, we replaced the --maxDistance parameter by --maxLibraryInsertSize, which should be based on the measured insert size of the Hi-C library.