CellMaker_DataParser

This repo contains code that parses raw CellMarker_Data into a JSON file that Dumper can use in Biothings Studio.

Table of Contents

1. About the dataset
2. Dataset Features
3. Data Processing Workflow
4. Export JSON file outline
5. Contact
6. Acknowledgments

About the dataset

![Product Name Screen Shot][product-screenshot]

About this dataset: The dataset can be found here. These records contain data about molecules expressed on the surface, within, or secreted by cells. These markers are used to identify and classify the types, states, or functions of cells within a population.

Dataset Schema

There in total 4 different different Cell_Marker Dataset but all of them have the same attributes.

• speciesType: the species from which the data originates
  • there are only two data type, either Human or Mouse
• tissueType: the type of tissues from which data originates
  • in total 181 different kinds of cells
  • a lot of them are undefined
• UberonOntologyID: The universal unique identifier of the anatomy structure found in animals
  • needs to confirm with the team
  • contain missing value and most of them are missing due to undefined tissueType
• cancerType: the association of the cell marker with the cancer name
  • if the cell Marker does not represent cancer, then it is named as Normal
• cellName: the English name of the cell that the marker belongs to
• CellOntologyID: The universal unique identifier of the cell that the marker belongs to
  • contain missing value
• cellMarker: a marker molecule of the cell
  • in string like list, can be converted to a list
• geneSymbol: gene expression of the cell marker
  • in string like list, can be converted to a list
• geneID: The universal unique identifier of the gene
  • in string like list, can be converted to a list
  • contain missing value
• proteinName: name of the protein
  • in string like list, can be converted to a list
  • contain missing value
• proteinID: The universal unique identifier of the protein
  • in string like list, can be converted to a list
• markerResource: the type of resource or methodology used to identify the marker
  • there are only four data types, either Experiment or Single-cell sequencing or Company or Review
• PMID: The PudMed ID for the publication or study where the marker data was reported
  • if the markerResource is value company, the the value here is contains company
• Company: the company associated with the resources
  • most of them are missing and only exist when the markerResource is Company

Data Set relationships

Dataset Features

This section will discuss some of the features of the data set. All of the work can be found in the (Jupyter Notebook) in the EDA folder.

Missingness of UberonOntologyID

Most of the missing UberonOntologyID is due to "Undefined" tissue-type

Missingness of CellOntologyID

Most of the missing CellOntologyID is due to "Cancer stem cell" in cellName

Missingness of geneSymbol and geneID

They either exist or are missing at the same time

Missingness of proteinName and proteinID

They either exist or are missing at the same time

list like str value in columns

In the following columns:

• geneSymbol
• geneID
• proteinName
• proteinID

values are stored in list-like strings. Here are Example of strings:

• "A"
• "A, B"
• "A B"
• "A, [A, B], C"
• "A, B, C, D, [E, F], [G, H I]"

We expected the parsing result to be:

• ['A']
• ['A', 'B']
• ['A B']
• ['A', ['A', 'B'], 'C']
• ['A', 'B', 'C', 'D', ['E', 'F'], ['G', 'H I']]

Missingness of company value

91% of the value in the company column is missing, but it is missing by design. There are in total 4 different kinds of values in markerResource column which are "Experiment", "Review", "Single-cell sequencing", and "Company". The company column is not missing when the value in markerResource column is "company"

Export JSON file outline

geneID

• geneSymbol: str
• proteinID: dict
  • proteinName
• cellMarker: dict
  • speciesType: str
  • tissueType: str
  • UberonOntologyID: str
  • cancerType: str
  • cellType: str
  • cellName: str
  • CellOntologyID: str
  • markerResource: tuple one of this
    • Experiment: PMID
    • Review: PMID
    • Single-cell sequencing: PMID
    • Company: Company name

Data Processing Workflow

• concatenating all_cell_markers df and all_singleCell_markers df
• replacing all the "undefined" tissue with NaN value
• converting all the listLikeString into the list for column [geneSymbol, geneID, proteinName, proteinName]
• remove all rows with missing "geneID"

Contact

Guoxuan Xu - @github_profile - [email protected]

Acknowledgments

• Apperciate Dr. Wu and Jason Lin for the help!