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* Add bwa-mem2 data manager * Drop python wrapper
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data_managers/data_manager_bwa_mem2_index_builder/.shed.yml
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categories: | ||
- Data Managers | ||
description: Bwa-mem2 is the next version of the bwa-mem algorithm in bwa. | ||
homepage_url: https://github.com/bwa-mem2/bwa-mem2 | ||
long_description: | | ||
Bwa-mem2 is the next version of the bwa-mem algorithm in bwa. It produces | ||
alignment identical to bwa and is ~1.3-3.1x faster depending on the use-case, | ||
dataset and the running machine. Bwa-mem2 uses a different index format that | ||
is efficient on disk space and runtime memory but requires larger amounts of | ||
memory (roughly 27x the reference) when building. | ||
name: data_manager_bwa_mem2_index_builder | ||
owner: iuc | ||
remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/data_managers/data_manager_bwa_mem2_index_builder | ||
type: unrestricted |
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data_managers/data_manager_bwa_mem2_index_builder/data_manager/bwa_mem2_index_builder.xml
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<tool id="bwa_mem2_index_builder_data_manager" name="Build BWA-MEM2 indexes" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" tool_type="manage_data" profile="22.05"> | ||
<description></description> | ||
<macros> | ||
<token name="@TOOL_VERSION@">2.2.1</token> | ||
<token name="@VERSION_SUFFIX@">0</token> | ||
</macros> | ||
<requirements> | ||
<requirement type="package" version="@TOOL_VERSION@">bwa-mem2</requirement> | ||
</requirements> | ||
<command detect_errors="exit_code"><![CDATA[ | ||
#set $fasta_file_name = str($all_fasta_source.fields.path).split('/')[-1] | ||
mkdir -p '${out_file.extra_files_path}' && | ||
ln -s '${all_fasta_source.fields.path}' '${out_file.extra_files_path}/${fasta_file_name}' && | ||
bwa-mem2 index '${out_file.extra_files_path}/${fasta_file_name}' && | ||
cp '$dmjson' '$out_file' | ||
]]> | ||
</command> | ||
<configfiles> | ||
<configfile name="dmjson"><![CDATA[#slurp | ||
#set $fasta_file_name = str($all_fasta_source.fields.path).split('/')[-1] | ||
#set $value = $sequence_id or $all_fasta_source.fields.dbkey | ||
#set $name = $sequence_name or $all_fasta_source.fields.name | ||
{ | ||
"data_tables":{ | ||
"bwa_mem2_indexes":[ | ||
{ | ||
"value": "${value}", | ||
"dbkey": "${all_fasta_source.fields.dbkey}", | ||
"name": "${name}", | ||
"path": "${fasta_file_name}" | ||
} | ||
] | ||
} | ||
} | ||
]]></configfile> | ||
</configfiles> | ||
<inputs> | ||
<param name="all_fasta_source" type="select" label="Source FASTA Sequence"> | ||
<options from_data_table="all_fasta"/> | ||
</param> | ||
<param name="sequence_name" type="text" value="" label="Name of sequence" /> | ||
<param name="sequence_id" type="text" value="" label="ID for sequence" /> | ||
</inputs> | ||
<outputs> | ||
<data name="out_file" format="data_manager_json" /> | ||
</outputs> | ||
<tests> | ||
<test> | ||
<param name="all_fasta_source" value="phiX174"/> | ||
<output name="out_file" file="bwa_mem2_data_manager.1.json"/> | ||
</test> | ||
<test> | ||
<param name="all_fasta_source" value="phiX174"/> | ||
<param name="sequence_name" value="Galeocerdo cuvier"/> | ||
<param name="sequence_id" value="fooBar1"/> | ||
<output name="out_file" file="bwa_mem2_data_manager.2.json"/> | ||
</test> | ||
</tests> | ||
<help> | ||
<![CDATA[ | ||
.. class:: infomark | ||
**Notice:** If you leave name, description, or id blank, it will be generated automatically. | ||
Bwa-mem2 is the next version of the bwa-mem algorithm in bwa. It produces | ||
alignment identical to bwa and is ~1.3-3.1x faster depending on the use-case, | ||
dataset and the running machine. Bwa-mem2 uses a different index format that | ||
is efficient on disk space and runtime memory but requires larger amounts of | ||
memory (roughly 27x the reference) when building. | ||
]]> | ||
</help> | ||
<citations> | ||
<citation type="doi">10.1038/nmeth.3317</citation> | ||
</citations> | ||
</tool> |
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data_managers/data_manager_bwa_mem2_index_builder/data_manager_conf.xml
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<?xml version="1.0"?> | ||
<data_managers> | ||
<data_manager tool_file="data_manager/bwa_mem2_index_builder.xml" id="bwa_mem2_index_builder"> | ||
<data_table name="bwa_mem2_indexes"> | ||
<output> | ||
<column name="value" /> | ||
<column name="dbkey" /> | ||
<column name="name" /> | ||
<column name="path" output_ref="out_file" > | ||
<move type="directory" relativize_symlinks="True"> | ||
<!-- <source>${path}</source>--> <!-- out_file.extra_files_path is used as base by default --> <!-- if no source, eg for type=directory, then refers to base --> | ||
<target base="${GALAXY_DATA_MANAGER_DATA_PATH}">${dbkey}/bwa_mem2_index/${value}</target> | ||
</move> | ||
<value_translation>${GALAXY_DATA_MANAGER_DATA_PATH}/${dbkey}/bwa_mem2_index/${value}/${path}</value_translation> | ||
<value_translation type="function">abspath</value_translation> | ||
</column> | ||
</output> | ||
</data_table> | ||
</data_manager> | ||
</data_managers> |
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data_managers/data_manager_bwa_mem2_index_builder/test-data/all_fasta.loc
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#This file lists the locations and dbkeys of all the fasta files | ||
#under the "genome" directory (a directory that contains a directory | ||
#for each build). The script extract_fasta.py will generate the file | ||
#all_fasta.loc. This file has the format (white space characters are | ||
#TAB characters): | ||
# | ||
#<unique_build_id> <dbkey> <display_name> <file_path> | ||
# | ||
#So, all_fasta.loc could look something like this: | ||
# | ||
#apiMel3 apiMel3 Honeybee (Apis mellifera): apiMel3 /path/to/genome/apiMel3/apiMel3.fa | ||
#hg19canon hg19 Human (Homo sapiens): hg19 Canonical /path/to/genome/hg19/hg19canon.fa | ||
#hg19full hg19 Human (Homo sapiens): hg19 Full /path/to/genome/hg19/hg19full.fa | ||
# | ||
#Your all_fasta.loc file should contain an entry for each individual | ||
#fasta file. So there will be multiple fasta files for each build, | ||
#such as with hg19 above. | ||
# | ||
phiX174 phiX174 phiX174 ${__HERE__}/phiX174.fasta |
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data_managers/data_manager_bwa_mem2_index_builder/test-data/bwa_mem2_data_manager.1.json
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{ | ||
"data_tables":{ | ||
"bwa_mem2_indexes":[ | ||
{ | ||
"value": "phiX174", | ||
"dbkey": "phiX174", | ||
"name": "phiX174", | ||
"path": "phiX174.fasta" | ||
} | ||
] | ||
} | ||
} |
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data_managers/data_manager_bwa_mem2_index_builder/test-data/bwa_mem2_data_manager.2.json
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{ | ||
"data_tables":{ | ||
"bwa_mem2_indexes":[ | ||
{ | ||
"value": "fooBar1", | ||
"dbkey": "phiX174", | ||
"name": "Galeocerdo cuvier", | ||
"path": "phiX174.fasta" | ||
} | ||
] | ||
} | ||
} |
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data_managers/data_manager_bwa_mem2_index_builder/test-data/phiX174.fasta
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>phiX174 | ||
GAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTTCGGATATTTCTGATGAGTCGAAAAATTATCTT | ||
GATAAAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAA | ||
ATTCGACCTATCCTTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAACGATTCTG | ||
TCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTA | ||
GATATGAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTGGATTACTATC | ||
TGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTT | ||
TCCAGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTT | ||
CGATTTTCTGACGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCT | ||
TGCGTTTATGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCG | ||
TCATTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTAC | ||
GGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTA | ||
CGCGCAGGAAACACTGACGTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAATTACGTGCAGAAGGAG | ||
TGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACT | ||
AAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC | ||
CCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCA | ||
TCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATTTCAACTACTCCGGTTATCGCTGGCGAC | ||
TCCTTCGAGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTA | ||
CTGTAGACATTTTTACTTTTTATGTCCCTCATCGTCACGTTTATGGTGAACAGTGGATTAAGTTCATGAA | ||
GGATGGTGTTAATGCCACTCCTCTCCCGACTGTTAACACTACTGGTTATATTGACCATGCCGCTTTTCTT | ||
GGCACGATTAACCCTGATACCAATAAAATCCCTAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACA | ||
ACTATTTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATGATGC | ||
TCGTTATGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTT | ||
TCTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC | ||
ATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACCGTGATGTTATTTCTTCATTTGGAGGTAAAAC | ||
CTCTTATGACGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCTATGATGTT | ||
GATGGAACTGACCAAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAACATTCTGTGC | ||
CGCGTTTCTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGCGAC | ||
TAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTTGACTTATACCGATATTGCTGGCGACCCTGTTTTG | ||
TATGGCAACTTGCCGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGT | ||
TTAAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTGA | ||
AGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGAT | ||
TATGACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGAATAGTCAGGTTAAATTTAATGTGACCGTTT | ||
ATCGCAATCTGCCGACCACTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATAAC | ||
GCCGAAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGCGGTAGGTTTTCTGC | ||
TTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGT | ||
TCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCTAAAGCTACATCGTCAACGTTA | ||
TATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGTTTTCTTCATTGCATTCAGATGGATACATCTG | ||
TCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGC | ||
CTGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTCCCGACTGCCTATGATGTTTATCCTTTG | ||
AATGGTCGCCATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGC | ||
CGGGCAATAATGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTACTAAATGCCGCGGATTGGT | ||
TTCGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTATGTTTGGTG | ||
CTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAA | ||
AGCCGCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACCGATAACAATACTGTAGGCATGGGTGATGCT | ||
GGTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCCCTAGTTTTGTTTCTG | ||
GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGA | ||
TAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTAT | ||
CTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGG | ||
TTGACGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA | ||
GATGCAAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTTCACGCCAGAATACGAAAGAC | ||
CAGGTATATGCACAAAATGAGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGCGTTGCGTCTATTA | ||
TGGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAAGCTCA | ||
AACGGCTGGTCAGTATTTTACCAATGACCAAATCAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGAC | ||
TTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCTCTTCTCATATTGGCGCTACTGCAAAGGATATTT | ||
CTAATGTCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCGA | ||
TACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTCTAATTTGTCTAGGAAATAACCG | ||
TCAGGATTGACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTTTTTATGGTTCGTT | ||
CTTATTACCCTTCTGAATGTCACGCTGATTATTTTGACTTTGAGCGTATCGAGGCTCTTAAACCTGCTAT | ||
TGAGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAATGCTTGGCTTCCATAAGCAGATGGATAACCGC | ||
ATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTATG | ||
TTGACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGA | ||
ATTGGCACAATGCTACAATGTGCTCCCCCAACTTGATATTAATAACACTATAGACCACCGCCCCGAAGGG | ||
GACGAAAAATGGTTTTTAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGCAAGCTGGCTGCTGAACGCC | ||
CTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATT | ||
GCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTACTATTCAGCGTTTGATGAATGCAATGCGACAG | ||
GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGAGGCGTTTT | ||
ATGATAATCCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCG | ||
CAAGGCTAATGATTCACACGCCGACTGCTATCAGTATTTTTGTGTGCCTGAGTATGGTACAGCTAATGGC | ||
CGTCTTCATTTCCATGCGGTGCATTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTC | ||
GTCGGGTACGCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT | ||
CGCAGTTCGCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAG | ||
CCGCTTAAAGCTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTAAATACGTTAACAAAAAGTCAGATA | ||
TGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACT | ||
TCCCAAGAAGCTGTTCAGAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATCTG | ||
TCCACGGAGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGC | ||
AGAACGCAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTTTGGCGGCGCAACC | ||
TGTGACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAATGATTGGCGTATCCAACCTGCA | ||
|
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data_managers/data_manager_bwa_mem2_index_builder/tool-data/all_fasta.loc.sample
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#This file lists the locations and dbkeys of all the fasta files | ||
#under the "genome" directory (a directory that contains a directory | ||
#for each build). The script extract_fasta.py will generate the file | ||
#all_fasta.loc. This file has the format (white space characters are | ||
#TAB characters): | ||
# | ||
#<unique_build_id> <dbkey> <display_name> <file_path> | ||
# | ||
#So, all_fasta.loc could look something like this: | ||
# | ||
#apiMel3 apiMel3 Honeybee (Apis mellifera): apiMel3 /path/to/genome/apiMel3/apiMel3.fa | ||
#hg19canon hg19 Human (Homo sapiens): hg19 Canonical /path/to/genome/hg19/hg19canon.fa | ||
#hg19full hg19 Human (Homo sapiens): hg19 Full /path/to/genome/hg19/hg19full.fa | ||
# | ||
#Your all_fasta.loc file should contain an entry for each individual | ||
#fasta file. So there will be multiple fasta files for each build, | ||
#such as with hg19 above. | ||
# |
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data_managers/data_manager_bwa_mem2_index_builder/tool-data/bwa_mem2_index.loc.sample
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#This is a sample file distributed with Galaxy that enables tools | ||
#to use a directory of BWA indexed sequences data files. You will need | ||
#to create these data files and then create a bwa_index.loc file | ||
#similar to this one (store it in this directory) that points to | ||
#the directories in which those files are stored. The bwa_index.loc | ||
#file has this format (longer white space characters are TAB characters): | ||
# | ||
#<unique_build_id> <dbkey> <display_name> <file_path> | ||
# | ||
#So, for example, if you had phiX indexed stored in | ||
#/depot/data2/galaxy/phiX/base/, | ||
#then the bwa_index.loc entry would look like this: | ||
# | ||
#phiX174 phiX phiX Pretty /depot/data2/galaxy/phiX/base/phiX.fa | ||
# | ||
#and your /depot/data2/galaxy/phiX/base/ directory | ||
#would contain phiX.fa.* files: | ||
# | ||
#-rw-r--r-- 1 james universe 830134 2005-09-13 10:12 phiX.fa.amb | ||
#-rw-r--r-- 1 james universe 527388 2005-09-13 10:12 phiX.fa.ann | ||
#-rw-r--r-- 1 james universe 269808 2005-09-13 10:12 phiX.fa.bwt | ||
#...etc... | ||
# | ||
#Your bwa_index.loc file should include an entry per line for each | ||
#index set you have stored. The "file" in the path does not actually | ||
#exist, but it is the prefix for the actual index files. For example: | ||
# | ||
#phiX174 phiX phiX174 /depot/data2/galaxy/phiX/base/phiX.fa | ||
#hg18canon hg18 hg18 Canonical /depot/data2/galaxy/hg18/base/hg18canon.fa | ||
#hg18full hg18 hg18 Full /depot/data2/galaxy/hg18/base/hg18full.fa | ||
#/orig/path/hg19.fa hg19 hg19 /depot/data2/galaxy/hg19/base/hg19.fa | ||
#...etc... | ||
# | ||
#Note that for backwards compatibility with workflows, the unique ID of | ||
#an entry must be the path that was in the original loc file, because that | ||
#is the value stored in the workflow for that parameter. That is why the | ||
#hg19 entry above looks odd. New genomes can be better-looking. | ||
# |
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data_managers/data_manager_bwa_mem2_index_builder/tool_data_table_conf.xml.sample
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<!-- Use the file tool_data_table_conf.xml.oldlocstyle if you don't want to update your loc files as changed in revision 4550:535d276c92bc--> | ||
<tables> | ||
<!-- Locations of indexes in the BWA-MEM2 mapper format--> | ||
<table name="bwa_mem2_indexes" comment_char="#"> | ||
<columns>value, dbkey, name, path</columns> | ||
<file path="tool-data/bwa_mem2_index.loc" /> | ||
</table> | ||
</tables> |
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data_managers/data_manager_bwa_mem2_index_builder/tool_data_table_conf.xml.test
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<tables> | ||
<!-- Locations of all fasta files under genome directory --> | ||
<table name="all_fasta" comment_char="#"> | ||
<columns>value, dbkey, name, path</columns> | ||
<file path="${__HERE__}/test-data/all_fasta.loc" /> | ||
</table> | ||
<!-- Locations of indexes in the BWA-MEM2 mapper format--> | ||
<table name="bwa_mem2_indexes" comment_char="#"> | ||
<columns>value, dbkey, name, path</columns> | ||
<file path="${__HERE__}/test-data/bwa_mem2_index.loc" /> | ||
</table> | ||
</tables> |