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Source code for the NeurIPS 2022 paper TANKBind: Trigonometry-Aware Neural NetworKs for Drug-Protein Binding Structure Prediction

TankBind

TankBind could predict both the protein-ligand binding structure and their affinity.

The primary purpose of this repository is to enable the reproduction of the results reported in the paper, as well as to facilitate the work of others who wish to build upon it. To experience the latest version, which includes various improvements made to the model, simply create an account at https://m1.galixir.com/public/login_en/index.html.

If you have any question or suggestion, please feel free to open an issue or email me at [email protected] or shuangjia zheng at [email protected].

Installation

conda create -n tankbind_py38 python=3.8
conda activate tankbind_py38

You might want to change the cudatoolkit version based on the GPU you are using.:

conda install pytorch cudatoolkit=11.3 -c pytorch
conda install torchdrug=0.1.2 pyg=2.1.0 biopython nglview jupyterlab -c milagraph -c conda-forge -c pytorch -c pyg
pip install torchmetrics tqdm mlcrate pyarrow
rdkit version used: 2021.03.4

p2rank v2.3 could be downloaded from here:

https://github.com/rdk/p2rank/releases/download/2.3/p2rank_2.3.tar.gz

Test set evaluation

We include the script for reproducing the self-dock result in

examples/testset_evaluation_cleaned.ipynb

The test_dataset is constructed using the notebook in "Dataset construction" section.

Prediction

We use the prediction of the structure of protein ABL1 in complex with two drugs, Imatinib and compound6 (PDB: 6HD6) as an example for predicting the drug-protein binding structure.

examples/prediction_example_using_PDB_6hd6.ipynb

Dataset construction

Scripts for training/test dataset construction is provided in:

examples/construction_PDBbind_training_and_test_dataset.ipynb.ipynb

The Script I used to train the model is

python main.py -d 0 -m 0 --batch_size 5 --label baseline --addNoise 5 --use_equivalent_native_y_mask

High-throughput virtual screening

TankBind also support virtual screening. In our example here, for the WDR domain of LRRK2 protein, we can screen 10,000 drug candidates in 2 minutes (or 1M in around 3 hours) with a single GPU. Check out

examples/high_throughput_virtual_screening_LRRK2_WDR.ipynb

Citation

@article{lu2022tankbind,
	title={Tankbind: Trigonometry-aware neural networks for drug-protein binding structure prediction},
	author={Lu, Wei and Wu, Qifeng and Zhang, Jixian and Rao, Jiahua and Li, Chengtao and Zheng, Shuangjia},
	journal={Advances in Neural Information Processing Systems},
	year={2022}
}

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Open source code for TankBind. Galixir Tenchnologies

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