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Merge pull request #23 from nextstrain/update-references-to-match-refseq
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Update references to match RefSeq
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trvrb authored Aug 15, 2024
2 parents 1f9660f + 6405c92 commit a2b2262
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Showing 10 changed files with 651 additions and 341 deletions.
8 changes: 8 additions & 0 deletions phylogenetic/defaults/config.yaml
Original file line number Diff line number Diff line change
Expand Up @@ -14,6 +14,7 @@ display_strain_field: "strain"

filter:
min_length: 500
include: "defaults/include.txt" # need to always include root strains
exclude: "defaults/exclude.txt"
query: "is_lab_host != 'true'"
# Necessary for is_lab_host to be read as a string instaed of a boolean
Expand All @@ -26,6 +27,13 @@ refine:
coalescent: "opt"
date_inference: "marginal"
clock_rate: 0.0006
# Rooting to outgroup clade based on strains
# Pinneo-NIG-1969 (L accession KM822127 and S accession KM822128)
# and strain 812285 (L accession MG812674 and S accession MG812675)
# TreeTime needs two tips to root to common ancestor of these tips
root:
l: "KM822127 MG812674"
s: "KM822128 MG812675"

ancestral:
inference: "joint"
Expand Down
4 changes: 4 additions & 0 deletions phylogenetic/defaults/include.txt
Original file line number Diff line number Diff line change
@@ -0,0 +1,4 @@
KM822127 # Pinneo-NIG-1969 L segment
KM822128 # Pinneo-NIG-1969 S segment
MG812674 # 812285 L segment
MG812675 # 812285 S segment
437 changes: 223 additions & 214 deletions phylogenetic/defaults/lassa_l.gb

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253 changes: 131 additions & 122 deletions phylogenetic/defaults/lassa_s.gb

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4 changes: 3 additions & 1 deletion phylogenetic/example_data/metadata_l.tsv
Original file line number Diff line number Diff line change
Expand Up @@ -10,4 +10,6 @@ G1727_SLE_2011 KM821809 L 2011-XX-XX africa sierra_leone human Andersen et al W
G2222_SLE_2011 KM821831 L 2011-XX-XX africa sierra_leone human Andersen et al Whole genome-sequencing from clinical and field samples uncovers ancient origins and intra-host evolution of Lassa virus Unpublished https://www.ncbi.nlm.nih.gov/pubmed/
G636_SLE_2009 KM821778 L 2009-XX-XX africa sierra_leone human Andersen et al Whole genome-sequencing from clinical and field samples uncovers ancient origins and intra-host evolution of Lassa virus Unpublished https://www.ncbi.nlm.nih.gov/pubmed/
G3248_SLE_2013 KM821905 L 2013-XX-XX africa sierra_leone human Andersen et al Whole genome-sequencing from clinical and field samples uncovers ancient origins and intra-host evolution of Lassa virus Unpublished https://www.ncbi.nlm.nih.gov/pubmed/
G2557_SLE_2012 KM821860 L 2012-XX-XX africa sierra_leone human Andersen et al Whole genome-sequencing from clinical and field samples uncovers ancient origins and intra-host evolution of Lassa virus Unpublished https://www.ncbi.nlm.nih.gov/pubmed/
G2557_SLE_2012 KM821860 L 2012-XX-XX africa sierra_leone human Andersen et al Whole genome-sequencing from clinical and field samples uncovers ancient origins and intra-host evolution of Lassa virus Unpublished https://www.ncbi.nlm.nih.gov/pubmed/
Pinneo-NIG-1969 KM822127 L 1969-XX-XX africa nigeria human Andersen et al https://www.ncbi.nlm.nih.gov/pubmed/
812285 MG812674 L 1976-XX-XX africa nigeria human Welch et al https://www.ncbi.nlm.nih.gov/pubmed/
4 changes: 3 additions & 1 deletion phylogenetic/example_data/metadata_s.tsv
Original file line number Diff line number Diff line change
Expand Up @@ -12,4 +12,6 @@ G2222_SLE_2011 KM821832 S 2011-XX-XX africa sierra_leone human Andersen et al W
G636_SLE_2009 KM821779 S 2009-XX-XX africa sierra_leone human Andersen et al Whole genome-sequencing from clinical and field samples uncovers ancient origins and intra-host evolution of Lassa virus Unpublished https://www.ncbi.nlm.nih.gov/pubmed/
G3248_SLE_2013 KM821906 S 2013-XX-XX africa sierra_leone human Andersen et al Whole genome-sequencing from clinical and field samples uncovers ancient origins and intra-host evolution of Lassa virus Unpublished https://www.ncbi.nlm.nih.gov/pubmed/
G2511_SLE_2012 KM821857 S 2012-XX-XX africa sierra_leone human Andersen et al Whole genome-sequencing from clinical and field samples uncovers ancient origins and intra-host evolution of Lassa virus Unpublished https://www.ncbi.nlm.nih.gov/pubmed/
G2557_SLE_2012 KM821861 S 2012-XX-XX africa sierra_leone human Andersen et al Whole genome-sequencing from clinical and field samples uncovers ancient origins and intra-host evolution of Lassa virus Unpublished https://www.ncbi.nlm.nih.gov/pubmed/
G2557_SLE_2012 KM821861 S 2012-XX-XX africa sierra_leone human Andersen et al Whole genome-sequencing from clinical and field samples uncovers ancient origins and intra-host evolution of Lassa virus Unpublished https://www.ncbi.nlm.nih.gov/pubmed/accession
Pinneo-NIG-1969 KM822128 S 1969-XX-XX africa nigeria human Andersen et al https://www.ncbi.nlm.nih.gov/pubmed/
812285 MG812675 S 1976-XX-XX africa nigeria human Welch et al https://www.ncbi.nlm.nih.gov/pubmed/
184 changes: 184 additions & 0 deletions phylogenetic/example_data/sequences_l.fasta
Original file line number Diff line number Diff line change
Expand Up @@ -1470,3 +1470,187 @@ TCTCATTGTCTGCTAAGTACTTTGATACTAAATCTTTAACACAAGCTATGTCTTCCTCCA
TGTTTGGCTGCAAATATGGTTATCGAAACTCTTGGAATATGTGGTCAGCTAGAGTAACGG
TTCACTATGGGACAGGGTCCCATAGGAGATGCAAGAGACACAGCTGGTTAATTAAAGACA
ATTAAATTGGATATCCTTAATGCCTAGGATCCTCGGTGCG
>KM822127
CACCAACCCCAGCAGAGAGCCCCCACAACACAGCCAAGCCCACCCAGCCCCCAGAACCGCCCACATCAGCAGGCATGGGC
AACAAGCAGACCAGGTCCCCACCCAAACCAGAGCACCCCAGACCAACCCTGCTACCCGACGCATCCCACCTGGGCCCCCA
ATTCTGCAAGAGCTGCTGGTTTGAGAACAAGGGACTGGTGGAGTGTAATAACCATTACCTCTGTCTAAACTGTCTCACAC
TGCTTCTCAGCGTGAGCGACAGATGTCCTATTTGTAAGATGCCCCTCCCCACCAAGCTGGCAGTCCGAACCCAACCAAGT
GCACCCCCACTCAACCAGGGCAACACTCAATCCTCCCCGCCCCCCTACAGCCCCTAACTCTCCCAAGACGGGCCCGCACC
CCACACCCAGGAACCCAACCAAACACACACACACAGACAAACACATAGAAAGACACACACACACGCACACACACCCACAG
TCCCCACCCGCACCCCCGGGGGGACCCCCCGCCGGGGGCCCCCCCGGGGGGCTCAGAGGCTGGCATCACTCAATGTCCTC
TACCTGCACCAGTGTGTCGAACAATTTAATGCACGTCCTTCCCTTCAATCTCAGCTTTCCACTGCTTGACTGGATCATCA
CTTCTTTTAGTGACTTACTATAACACAGTGAGTAGCCCTTGAAATCCACCCAGTCACCAACTGCTTCAAGAAGCCGAACA
AGAATTGGTCTTTGCAATTCTAACACAGTCTTCTTCAACACAGAAACAATGTCGACATCCACCCAGTGCTTCTTGAGCTT
CAATCTGTCAGAAAACATTGAGATCAGTGCCTCTATCAAAAGATCTTGGGAACCATCCCTAACCTCCCTTATGAACAACT
CCAAGTCTTGATCGTGAATTGCTGGGGTAAAAGTAGCAAGTCTGCTGTCACATTCCCAAAGGGCTCCTCTACTGATCACT
AATGGTACAGGTTCAATTTCAGGCCCCACCAGTGTGAAATCATACTGTGAAAACTGCAGCTTCCTCAGATCAACAACTAT
CTCAATGGGGGTTACAGGCCCTAATTCTTTAGTGTCCTCTAGGAACTCATTAATGACCTCAGTATCGACAAACCATGCAG
ATTTCCCCTTGAAAACAGATCCAGACAGCACAAGCCTCCAGCAGTCAGGCAGCACACATTGGTTGACTGATCCGGAAAAC
ATGGCTTCAACATTATCAACTAAATACTTACCCTGATGATTTATTGAGCCAATGAAATTAAGATGAATAGAAAAATTTTC
TTTAAAACTTGCGTCTTGGCTTCTGATTTGGAATCTTATTGTCTGGCTGAATCTCACTGAACCAACTGGTAGTTCACTGA
TTCCATCTAAGAAGTCTTCAAGACATTTAAATTTGACATCAGTTAACCCACACATGATCAACTGACTGTAATTGAGTTTT
GCTGGTTCTAAATCATAATCAATCCATGCATAACCCATCATCAAATCATATTTGAACATAGGTCTTTCTATACCTTTGTA
TACCACCTTCTCAACAAAAGAGGAAAAGGGGCCAAGTCTCTGCAAACCTTCACCCTCTGGGATAGAGGTTTTATGAGGGA
ACCATGTAAATGATCCAAGCGTTCTAGTTGTTGCAACAAACGATCTGATGTACGACTCAAAATAAATCTGTCTCATAAAA
TTATCAACAACGTCACTCGAGCTGACAACCCTTTCTTCTATCATGGGTTCATGTGTCCTGGTGTGTGACAATCTCAGCTC
AGATGAAAGCACTATGTAGTGTTCCTCTCTCTTCCACTTCACTATGTGCGAGACAAGAGATAGTGCCTCGCAATTCACAT
CTAGTGCAACACAAAGATCTAGAAATTTAATTCTAGGTGACCACTTCATTTTAGTCGAGGCCAAATCACTCATGAAGGGC
AACAAATGTTTCTCAAACAGACTGGGGTACAGTCTCCTTAATGAGTGTATTATATGGTTCATGCCAACTCTATCTTCTAG
CAATTTAGAAGCAGTAGGTTTTAAGGGAAAGTAGTCACATGGGTTATGTTTGAAAAACTCCAGCTGTCCTGCTGGCTTAG
GGTTCCCTAGAACCCATGCCCCCAACTCTATCGCTGTTGATAATGAAATGCACATATAATCCCATAACAAAGATCTGAAA
TAAGATAGGACACTCTTCCCTTCAGACTCTTCTCTTACCCTCATTGGGACCCTCCAAATCTGGACTCCAAGGTCGTTAGT
TAAACACTCAACGCCCTGAATTGCCAGGACCTCAGATTGCAGTGATTTGATGTATAGGTTTTCCTTGTTAGACCCCCTCA
CACACTTGCTGCCAAGTGTTCTGCATAATCCTACAAAGCCTGAAGCGATTGAACTTTGGAAAGCAGATTTATTTATGGCC
TCAGATAGCAGTTTCTGAGCTCCTCTAGTGAATGTAGAAGACAATTTATTCTGAATGGTTTTCACTATTGTTGGTATTCT
CTCATCATCTAAACTCACTGCCCCAGCATAAATCACCTTCTGTCTCAGCACCATTCTTAATGGGTGATGTGTAGCCAGAT
TCAACCAACAAATTTCTAGATCAGAGTCATCCAACATCTCAGTACCCATTAACTCGCATAAATTCCTTAATGAAGAAACA
TGTTCACCTGACAGATAATTGGTGGTAAATTCCTCATGCAACTCACCTGTCTTTAATTTGTTAAACAACTTTCTTAGCAT
TGATCTTACCTTACTACAAGCCTCTGGGGCCAACTCTTCAATCAGTCTCATGATCCTGTAACTACGGTTGCCGTCAACCC
AATCCCTAACATCTGTGTTGCAATTGAGTAAGAATGGGTCAATGGGGTACCTCGCATACTTCAGCAAGTTCAAAGTTCTC
TTCTGAATAAGATTACATAGGCTAACTGGAACACCGTTAGCAATGGACTGATCAATTATGGTGTCAATTGTCTCTGCTAA
CTGGTGTGGTTCTTTACACTTAACATTATGTAGCGCAGCAGCCACAAACTTCGTCAAGAGGGGAACCTCATCACCCCAGA
CATAAAATCTTGATTTAAATTCTGCTACAAATCTTCCAATCACACTTTTCGGGCTGACAAACTTATTTAACTGATCACTC
ATATAGTAATGAAATTCTAACAGGGTTTTAAACTCATCTTGGTCTCTAGACATTAACTCTGTCAGGTTCTGGTCAAAAAG
AGAAATTTGGTCATCACTAGAAGTGTAAGCATCAACTTGCCCTCCACAAACACAACTCACAGCATAGTTTATAAATCTCT
CCGAAATGAGTCCATAAAAGTCTGAAGTGTTGTGCAGAATACCTTGCCCCATGTCAAGGATGGAACTTATATGAGATGGC
ACAACACCCAAATGAAAGTTTGAGTAAAAGAAATCTTCTGTTAGTGTCTGAGATGTACTCTTTCTCAACCCAAGTTGTGC
CTTTATATAAGATTTCATCATTGCTGACACTACATTAAAGGGAATTTCTACCATCTTGTGCATGTGCCATGCCAATAGTG
TTGAGAGATAATCCTTTCCTTTGGCATCAGCCTGAGTGTCCCCAGTGAGTAGAATTAAGTTCTGTAGGGTTGCAAGGAAT
AGGAATGGGCACATCATAGGACCCCATTTGCTGTGGTCCATGCTGTAGGATACATGTGCTTGTGAGACATTTAATTTCAT
TGACAAAATTGCATTCTCAAACTCTCTTTCATCATTTAAACAACTTCCCGAGAATTGCGTCGTCAATGCTTCAAAATAAT
CCTCTATCAATCTTGTGAACATTTTGGTCCTAAGGTCTCCAATATAAAGTTCTCTATTACCTCCAACCTGCTCTTTGTAT
GACAGCGAGAATTTTAGTCTCCCTGTATCAGGACCAACTGAGTTGAATGATTGTGGAGATTCCTGGCTATAAAAACACAG
ATTTTTCAACATGGCAGTTGTGCAATTTGTTAGTGAGAGTGCTTTACTAAGTGCTTCGGAGTTGCTCTCTCTTTCACTTA
TTCTAGTGTCGGTTGACAGTTTGTCTGTGTCAAATTTGAAATTGAGACACTTCGACTTATAGTGGGTATACCTCCCCATC
AACCTGTTGCCATTCATCAACAAGAGAATTGATTTGAAACACAGGAAATATTCCTGATCTGAATAAGTCCTGGTTACAAC
TGCTTTTGTTAGTTCACCAATAGGGCAAGACACCATGGGTCCACAATAAAAGTACTTCTGCTTAAACTCTTGGTGGTGAT
ACACTATGTCACAGAACTCTTTATAAAAAGTGTCAGGGAGTGTGTTATAATCAAAGTCCTCCACCATGTGGTTTGAGAGT
TCCCCTTTAATCAATCTGATGTACAGTTTTTCCTTAACAATCTCATTTAAATCGTCAATTGAACAAATCTCCCCGGTTGT
TGAGCGCTGGTCCGGTCCTTTCAGATCTCTATACTGATCCACAATTTTTTCTATAGTTTCTTTTAACTCATCAAAATATG
ACATCGCATTCCCTTCAAGCAATATCTCATCCAGGTCGGCCCTGTCTGTTTCTACATCTTTCCCTTGAGACCCCAAAACC
AAATTGCTCATAGCCTGCTGGACTTTGTACTCATAGTCTTGCTTATCTAATAAATACTTACCTTTCCTTGAAAAAACCTC
GGTCAATTGTGTAACTGCTAAGGCTGTTAGCTTGTTGAAATCATAATTTAAGACCCTACATCCATCTGTGTACTTATTAA
TCACAACACTCTTATTGCTTGCCAGATCTAAAGCAGTCGCACAGCCACTGGTTGACAGAGGGTCTTTTAATTCTTTCCTC
ACTTCTTTTCCTTTAAAGAGTGAACCATTGTTAAAAGAAGATGTAAGCAAGGACAAGTACTTCTTGGAGACACCAGGTTT
CTTGTACCTTATTTCAGCTGATTCCACACAGCATTCCTTACCTAAGAACTTTTTAGCGTTGTACACCATTTCACTCAACT
CATCCGACTCTGCATGATCCAGAGGATTGACGCTAACATGCCCAAACTCTAGTTTTGGTTCTAAGAATTTCTCAAAGCAC
TTGATCTGATCTGTTAGTCTATCAGGGGTTTCCTTAGTGATAAAATGACACATGTAAGAAATGTTCAAAACAAATTTGAA
CCTGTTTGTTAGCATACTAGTCACATTAGGAGATAAAACAGTGCTTAACAGGGCTCTTACCACTCTGAAAAGCAAGTACT
CAACATCTGTAATCAGCTTCTCTTTGATCTTGTTGATTAAGTCTTTGTGATAATAATCAGAGACAAAGGCCATTATGAAG
TATCTAAGATTTTGCAAAAACTTCTGACATCGCTTACTAGGGTGGGTCAAGATTAAAACCAAAATCATTTTGGTTAGCAC
TTTAATTGAGGATATATCATCCTTAAGCTCAGAACAATCTTCAATCCAGCTGACCATGACTTCTACCACCTTTGTCAGAA
CTTCACTGGAAAAGATTGCGGGGAAAAACCTCTTGGGATCCGCATAAAAGGAACAGACTTCACCAACTAAGTTGTTGTTT
ACCGCATAACATTTTGAACACTCGCCCGTTTTTTGATAAAGTAAGCTATATTCCACTCCATCTATGAAGAACTGTTGACA
GTATGCTTCTTTGCATCTTACCTTCTGGTATCTTGCTGCTCCAAGCTCATTTTGCCTCAACTTAACAGTGGAGGAAGTCT
TCATTGAATTCACCAATGCAAGACTCAATGTTGAGAGCCTTTCAAGATCAGCTGACTCTGAAACGCTCACTGTGCCCATT
GAGTAGGGAAACAGTCCTTCGTCAGACTTTTGATAAGACATTGTTGGCACAACACCTGATGCCTCACAGTTCATAACTTT
ACTAAATACATGCCCATCCAGAATTGTTAGATCAACCCCATAGGTGTCCACATTTACATTTAGGTCTTTGAGGGCACTTA
TTGCTTTGGTAACAGTTTTCTGCAACATGCAACTCAAGATTTGATTTCTATCTAATCTAACGGAGTGAGTGCTTGATGCT
GATTTTGAACATTCAGATTGCAATCTTCTATCAACCCACCTTTTCAGGTCCCTCTTAGTGTATTCTAAAGACACCAAGCG
GTCATTCACACTGATAAAACTGGATCCTAACCAGCCTTCCTGAGCTTGATCTTCACATTTAATCTTGTCCCTCTCATAAG
CAAGGAGAATCATTACATCAAAAATCAATAGAAGTTTCCTTCTTGTGTTCAAAATTCTCAAAGATTTGACTTTGTTGAGG
AATGACCTCCAACAATGCATCTGACTGCTGTTTGTGGGTGATGATCTATCAACCCCATTGGTTAAATCTGCATACAAGAA
TTTCAACACAGGAGAGGCTCTTTTAAAGGAATACATCAATTCTTCCACTCCATCTTCATTAGAAACCATTTCAGAATCAT
ACAAGCAGTGAAATTCTTTTAACAGTCTTTCTTTGTCAACTTTAACAAATTGATCTTCTATTTGGCCCTGCCTTAACTTG
TTTCTGAATATCTGATACTCCTCCTCAATCTGATTCTTAACCTCATGGGCAGTCATTTTGTTGTTGATGCCTTGATGGCA
GCTGGCAATTATATCATCAAAATGGTTGGTTCGTTTATCTAATAACACATTGATGCTTTCTATACCTGAAAACCGGCTAG
TGCTGACTGAAAGAGATTCGCATAATCTAGAGTACTCAGACTCTTCAAAGAGTGAATTGCTCTCCTGAGCATATTTCATC
AAGGAGAATAGTGTATCTCTGAGTTTGTCATTCACCCAGTCCGGAATTTGTTCATTATAAAATGTAGTTCTTCCGTCAAT
AAGTGGTATTAGGTTTATATCAATTGACTTCAAGTCATTCTTTAGTTGCTCTAACTTCTTTAAGTCCTCTAGGTACTTCT
GCTCAAAGTTCACTGGGGATGATCTCACAAAGCACTCAAGGAGTATGAGGACATTCCCATTCAATTTGAAGCCATCAGGC
ACAACAAAGCATAATGATGGTGTTAAAATCCCTAATTCATGGAGAATTACCTCAACAGAGCGTGCATTGCTGTTGTGCTC
ACAACCATTGGCCTTGCAGGAATCAACCTCTATACATAAGGATAAAAGTTTTAGTCCTTCCATCAACAACATCCTCGGCT
CAGTCTGCACCAAGAATGCCAATTTTTGTCTAGATAATCTCTCATCATCAGAAAGATATTTTGAAACAAGATCTTTTACA
TAAGCTATCTCCTCCTCCATTAAAGAAGTTGTCAACAATAGAGCGTCTAAATGCCTAGGATCCTCGGTGCGC
>MG812674
CACCAACCCCAGCAGAGAGCCCCCACAACACAGCCAAGCCCACCCAGCCCCCAGAACCGCCCACATCAGCAGGCATGGGC
AACAAGCAGACCAGGTCCCCACCCAAACCAGAGCACCCCAGACCAACCCTGCTACCCGACGCATCCCACCTGGGCCCCCA
ATTCTGCAAGAGCTGCTGGTTTGAGAACAAGGGACTGGTGGAGTGTAATAACCATTACCTCTGTCTAAACTGTCTCACAC
TGCTTCTCAGCGTGAGCGACAGATGTCCTATTTGTAAGATGCCCCTCCCCACCAAGCTGGCAGTCCGAACCCAACCAAGT
GCACCCCCACTCAACCAGGGCAACACTCAATCCTCCCCGCCCCCCTACAGCCCCTAACTCTCCCAAGACGGGCCCGCACC
CCACACCCAGGAACCCAACCAAACACACACACACAGACAAACACATAGAAAGACACACACACACGCACACACACCCACAG
TCCCCACCCGCACCCCCGGGGGGACCCCCCGCCGGGGGCCCCCCCGGGGGGCTCAGAGGCTGGCATCACTCAATGTCCTC
TACCTGCACCAGTGTGTCGAACAATTTAATGCACGTCCTTCCCTTCAATCTCAGCTTTCCACTGCTTGACTGGATCATCA
CTTCTTTTAGTGACTTACTATAACACAGTGAGTAGCCCTTGAAATCCACCCAGTCACCAACTGCTTCAAGAAGCCGAACA
AGAATTGGTCTTTGCAATTCTAACACAGTCTTCTTCAACACAGAAACAATGTCGACATCCACCCAGTGCTTCTTGAGCTT
CAATCTGTCAGAAAACATTGAGATCAGTGCCTCTATCAAAAGATCTTGGGAACCATCCCTAACCTCCCTTATGAACAACT
CCAAGTCTTGATCGTGAATTGCTGGGGTAAAAGTAGCAAGTCTGCTGTCACATTCCCAAAGGGCTCCTCTACTGATCACT
AATGGTACAGGTTCAATTTCAGGCCCCACCAGTGTGAAATCATACTGTGAAAACTGCAGCTTCCTCAGATCAACAACTAT
CTCAATGGGGGTTACAGGCCCTAATTCTTTAGTGTCCTCTAGGAACTCATTAATGACCTCAGTATCGACAAACCATGCAG
ATTTCCCCTTGAAAACAGATCCAGACAGCACAAGCCTCCAGCAGTCAGGCAGCACACATTGGTTGACTGATCCGGAAAAC
ATGGCTTCAACATTATCAACTAAATACTTACCCTGATGATTTATTGAGCCAATGAAATTAAGATGAATAGAAAAATTTTC
TTTAAAACTTGCGTCTTGGCTTCTGATTTGGAATCTTATTGTCTGGCTGAATCTCACTGAACCAACTGGTAGTTCACTGA
TTCCATCTAAGAAGTCTTCAAGACATTTAAATTTGACATCAGTTAACCCACACATGATCAACTGACTGTAATTGAGTTTT
GCTGGTTCTAAATCATAATCAATCCATGCATAACCCATCATCAAATCATATTTGAACATAGGTCTTTCTATACCTTTGTA
TACCACCTTCTCAACAAAAGAGGAAAAGGGGCCAAGTCTCTGCAAACCTTCACCCTCTGGGATAGAGGTTTTATGAGGGA
ACCATGTAAATGATCCAAGCGTTCTAGTTGTTGCAACAAACGATCTGATGTACGACTCAAAATAAATCTGTCTCATAAAA
TTATCAACAACGTCACTCGAGCTGACAACCCTTTCTTCTATCATGGGTTCATGTGTCCTGGTGTGTGACAATCTCAGCTC
AGATGAAAGCACTATGTAGTGTTCCTCTCTCTTCCACTTCACTATGTGCGAGACAAGAGATAGTGCCTCGCAATTCACAT
CTAGTGCAACACAAAGATCTAGAAATTTAATTCTAGGTGACCACTTCATTTTAGTCGAGGCCAAATCACTCATGAAGGGC
AACAAATGTTTCTCAAACAGACTGGGGTACAGTCTCCTTAATGAGTGTATTATATGGTTCATGCCAACTCTATCTTCTAG
CAATTTAGAAGCAGTAGGTTTTAAGGGAAAGTAGTCACATGGGTTATGTTTGAAAAACTCCAGCTGTCCTGCTGGCTTAG
GGTTCCCTAGAACCCATGCCCCCAACTCTATCGCTGTTGATAATGAAATGCACATATAATCCCATAACAAAGATCTGAAA
TAAGATAGGACACTCTTCCCTTCAGACTCTTCTCTTACCCTCATTGGGACCCTCCAAATCTGGACTCCAAGGTCGTTAGT
TAAACACTCAACGCCCTGAATTGCCAGGACCTCAGATTGCAGTGATTTGATGTATAGGTTTTCCTTGTTAGACCCCCTCA
CACACTTGCTGCCAAGTGTTCTGCATAATCCTACAAAGCCTGAAGCGATTGAACTTTGGAAAGCAGATTTATTTATGGCC
TCAGATAGCAGTTTCTGAGCTCCTCTAGTGAATGTAGAAGACAATTTATTCTGAATGGTTTTCACTATTGTTGGTATTCT
CTCATCATCTAAACTCACTGCCCCAGCATAAATCACCTTCTGTCTCAGCACCATTCTTAATGGGTGATGTGTAGCCAGAT
TCAACCAACAAATTTCTAGATCAGAGTCATCCAACATCTCAGTACCCATTAACTCGCATAAATTCCTTAATGAAGAAACA
TGTTCACCTGACAGATAATTGGTGGTAAATTCCTCATGCAACTCACCTGTCTTTAATTTGTTAAACAACTTTCTTAGCAT
TGATCTTACCTTACTACAAGCCTCTGGGGCCAACTCTTCAATCAGTCTCATGATCCTGTAACTACGGTTGCCGTCAACCC
AATCCCTAACATCTGTGTTGCAATTGAGTAAGAATGGGTCAATGGGGTACCTCGCATACTTCAGCAAGTTCAAAGTTCTC
TTCTGAATAAGATTACATAGGCTAACTGGAACACCGTTAGCAATGGACTGATCAATTATGGTGTCAATTGTCTCTGCTAA
CTGGTGTGGTTCTTTACACTTAACATTATGTAGCGCAGCAGCCACAAACTTCGTCAAGAGGGGAACCTCATCACCCCAGA
CATAAAATCTTGATTTAAATTCTGCTACAAATCTTCCAATCACACTTTTCGGGCTGACAAACTTATTTAACTGATCACTC
ATATAGTAATGAAATTCTAACAGGGTTTTAAACTCATCTTGGTCTCTAGACATTAACTCTGTCAGGTTCTGGTCAAAAAG
AGAAATTTGGTCATCACTAGAAGTGTAAGCATCAACTTGCCCTCCACAAACACAACTCACAGCATAGTTTATAAATCTCT
CCGAAATGAGTCCATAAAAGTCTGAAGTGTTGTGCAGAATACCTTGCCCCATGTCAAGGATGGAACTTATATGAGATGGC
ACAACACCCAAATGAAAGTTTGAGTAAAAGAAATCTTCTGTTAGTGTCTGAGATGTACTCTTTCTCAACCCAAGTTGTGC
CTTTATATAAGATTTCATCATTGCTGACACTACATTAAAGGGAATTTCTACCATCTTGTGCATGTGCCATGCCAATAGTG
TTGAGAGATAATCCTTTCCTTTGGCATCAGCCTGAGTGTCCCCAGTGAGTAGAATTAAGTTCTGTAGGGTTGCAAGGAAT
AGGAATGGGCACATCATAGGACCCCATTTGCTGTGGTCCATGCTGTAGGATACATGTGCTTGTGAGACATTTAATTTCAT
TGACAAAATTGCATTCTCAAACTCTCTTTCATCATTTAAACAACTTCCCGAGAATTGCGTCGTCAATGCTTCAAAATAAT
CCTCTATCAATCTTGTGAACATTTTGGTCCTAAGGTCTCCAATATAAAGTTCTCTATTACCTCCAACCTGCTCTTTGTAT
GACAGCGAGAATTTTAGTCTCCCTGTATCAGGACCAACTGAGTTGAATGATTGTGGAGATTCCTGGCTATAAAAACACAG
ATTTTTCAACATGGCAGTTGTGCAATTTGTTAGTGAGAGTGCTTTACTAAGTGCTTCGGAGTTGCTCTCTCTTTCACTTA
TTCTAGTGTCGGTTGACAGTTTGTCTGTGTCAAATTTGAAATTGAGACACTTCGACTTATAGTGGGTATACCTCCCCATC
AACCTGTTGCCATTCATCAACAAGAGAATTGATTTGAAACACAGGAAATATTCCTGATCTGAATAAGTCCTGGTTACAAC
TGCTTTTGTTAGTTCACCAATAGGGCAAGACACCATGGGTCCACAATAAAAGTACTTCTGCTTAAACTCTTGGTGGTGAT
ACACTATGTCACAGAACTCTTTATAAAAAGTGTCAGGGAGTGTGTTATAATCAAAGTCCTCCACCATGTGGTTTGAGAGT
TCCCCTTTAATCAATCTGATGTACAGTTTTTCCTTAACAATCTCATTTAAATCGTCAATTGAACAAATCTCCCCGGTTGT
TGAGCGCTGGTCCGGTCCTTTCAGATCTCTATACTGATCCACAATTTTTTCTATAGTTTCTTTTAACTCATCAAAATATG
ACATCGCATTCCCTTCAAGCAATATCTCATCCAGGTCGGCCCTGTCTGTTTCTACATCTTTCCCTTGAGACCCCAAAACC
AAATTGCTCATAGCCTGCTGGACTTTGTACTCATAGTCTTGCTTATCTAATAAATACTTACCTTTCCTTGAAAAAACCTC
GGTCAATTGTGTAACTGCTAAGGCTGTTAGCTTGTTGAAATCATAATTTAAGACCCTACATCCATCTGTGTACTTATTAA
TCACAACACTCTTATTGCTTGCCAGATCTAAAGCAGTCGCACAGCCACTGGTTGACAGAGGGTCTTTTAATTCTTTCCTC
ACTTCTTTTCCTTTAAAGAGTGAACCATTGTTAAAAGAAGATGTAAGCAAGGACAAGTACTTCTTGGAGACACCAGGTTT
CTTGTACCTTATTTCAGCTGATTCCACACAGCATTCCTTACCTAAGAACTTTTTAGCGTTGTACACCATTTCACTCAACT
CATCCGACTCTGCATGATCCAGAGGATTGACGCTAACATGCCCAAACTCTAGTTTTGGTTCTAAGAATTTCTCAAAGCAC
TTGATCTGATCTGTTAGTCTATCAGGGGTTTCCTTAGTGATAAAATGACACATGTAAGAAATGTTCAAAACAAATTTGAA
CCTGTTTGTTAGCATACTAGTCACATTAGGAGATAAAACAGTGCTTAACAGGGCTCTTACCACTCTGAAAAGCAAGTACT
CAACATCTGTAATCAGCTTCTCTTTGATCTTGTTGATTAAGTCTTTGTGATAATAATCAGAGACAAAGGCCATTATGAAG
TATCTAAGATTTTGCAAAAACTTCTGACATCGCTTACTAGGGTGGGTCAAGATTAAAACCAAAATCATTTTGGTTAGCAC
TTTAATTGAGGATATATCATCCTTAAGCTCAGAACAATCTTCAATCCAGCTGACCATGACTTCTACCACCTTTGTCAGAA
CTTCACTGGAAAAGATTGCGGGGAAAAACCTCTTGGGATCCGCATAAAAGGAACAGACTTCACCAACTAAGTTGTTGTTT
ACCGCATAACATTTTGAACACTCGCCCGTTTTTTGATAAAGTAAGCTATATTCCACTCCATCTATGAAGAACTGTTGACA
GTATGCTTCTTTGCATCTTACCTTCTGGTATCTTGCTGCTCCAAGCTCATTTTGCCTCAACTTAACAGTGGAGGAAGTCT
TCATTGAATTCACCAATGCAAGACTCAATGTTGAGAGCCTTTCAAGATCAGCTGACTCTGAAACGCTCACTGTGCCCATT
GAGTAGGGAAACAGTCCTTCGTCAGACTTTTGATAAGACATTGTTGGCACAACACCTGATGCCTCACAGTTCATAACTTT
ACTAAATACATGCCCATCCAGAATTGTTAGATCAACCCCATAGGTGTCCACATTTACATTTAGGTCTTTGAGGGCACTTA
TTGCTTTGGTAACAGTTTTCTGCAACATGCAACTCAAGATTTGATTTCTATCTAATCTAACGGAGTGAGTGCTTGATGCT
GATTTTGAACATTCAGATTGCAATCTTCTATCAACCCACCTTTTCAGGTCCCTCTTAGTGTATTCTAAAGACACCAAGCG
GTCATTCACACTGATAAAACTGGATCCTAACCAGCCTTCCTGAGCTTGATCTTCACATTTAATCTTGTCCCTCTCATAAG
CAAGGAGAATCATTACATCAAAAATCAATAGAAGTTTCCTTCTTGTGTTCAAAATTCTCAAAGATTTGACTTTGTTGAGG
AATGACCTCCAACAATGCATCTGACTGCTGTTTGTGGGTGATGATCTATCAACCCCATTGGTTAAATCTGCATACAAGAA
TTTCAACACAGGAGAGGCTCTTTTAAAGGAATACATCAATTCTTCCACTCCATCTTCATTAGAAACCATTTCAGAATCAT
ACAAGCAGTGAAATTCTTTTAACAGTCTTTCTTTGTCAACTTTAACAAATTGATCTTCTATTTGGCCCTGCCTTAACTTG
TTTCTGAATATCTGATACTCCTCCTCAATCTGATTCTTAACCTCATGGGCAGTCATTTTGTTGTTGATGCCTTGATGGCA
GCTGGCAATTATATCATCAAAATGGTTGGTTCGTTTATCTAATAACACATTGATGCTTTCTATACCTGAAAACCGGCTAG
TGCTGACTGAAAGAGATTCGCATAATCTAGAGTACTCAGACTCTTCAAAGAGTGAATTGCTCTCCTGAGCATATTTCATC
AAGGAGAATAGTGTATCTCTGAGTTTGTCATTCACCCAGTCCGGAATTTGTTCATTATAAAATGTAGTTCTTCCGTCAAT
AAGTGGTATTAGGTTTATATCAATTGACTTCAAGTCATTCTTTAGTTGCTCTAACTTCTTTAAGTCCTCTAGGTACTTCT
GCTCAAAGTTCACTGGGGATGATCTCACAAAGCACTCAAGGAGTATGAGGACATTCCCATTCAATTTGAAGCCATCAGGC
ACAACAAAGCATAATGATGGTGTTAAAATCCCTAATTCATGGAGAATTACCTCAACAGAGCGTGCATTGCTGTTGTGCTC
ACAACCATTGGCCTTGCAGGAATCAACCTCTATACATAAGGATAAAAGTTTTAGTCCTTCCATCAACAACATCCTCGGCT
CAGTCTGCACCAAGAATGCCAATTTTTGTCTAGATAATCTCTCATCATCAGAAAGATATTTTGAAACAAGATCTTTTACA
TAAGCTATCTCCTCCTCCATTAAAGAAGTTGTCAACAATAGAGCGTCTAAATGCCTAGGATCCTCGGTGCG-
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