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| ## Unreleased | ||
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| Initial release of the dataset. |
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| # Human Metapneumovirus - All Clades with Reference Genome NC_039199 | ||
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| | Key | Value | | ||
| | ---------------------- | --------------------------------------------------------------------------------------------------------------------| | ||
| | authors | Isabel Joia, [Richard Neher](https://neherlab.org), [Nextstrain](https://nextstrain.org) | | ||
| | data source | Genbank | | ||
| | workflow | [github.com/nextstrain/human-metapneumovirus](https://github.com/nextstrain/human-metapneumovirus) | | ||
| | nextclade dataset path | nextstrain/hmpv/all-clades/NC_039199 | | ||
| | reference | NC_039199 | | ||
| | clade definitions | [github.com/nextstrain/human-metapneumovirus/nextclade/clades.tsv](https://github.com/nextstrain/human-metapneumovirus/blob/main/nextclade/resources/clades.tsv) | | ||
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| ## Scope of this dataset | ||
| This dataset for human metapneumovirus uses reference sequence [NC_039199](https://www.ncbi.nlm.nih.gov/nuccore/NC_039199.1/) which is available at NCBI RefSeq. The reference tree covers the diversity of human metapneumovirus since the first sequenced samples. | ||
| The reference tree is mid-point rooted. | ||
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| **Note: The reference sequence belongs to clade A1 and extensive divergence between clades A and B may render alignment in gene G unreliable for sequences in clade B.** | ||
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| ### Features | ||
| This dataset supports: | ||
| - Assignment to lineage nomenclature initially proposed by [van den Hoogen et al, 2004](https://wwwnc.cdc.gov/eid/article/10/4/03-0393_article) | ||
| - Phylogenetic placement | ||
| - Sequence QC (though frame shifts in the G-protein can not be accurately called across all of HMPV diversity) | ||
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data/nextstrain/hmpv/all-clades/NC_039199/genome_annotation.gff3
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| ##gff-version 3 | ||
| #!gff-spec-version 1.21 | ||
| #!processor NCBI annotwriter | ||
| #!genome-build ASM281537v1 | ||
| #!genome-build-accession NCBI_Assembly:GCF_002815375.1 | ||
| ##sequence-region NC_039199.1 1 13350 | ||
| ##species https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=162145 | ||
| NC_039199.1 RefSeq region 1 13350 . + . ID=NC_039199.1:1..13350;Dbxref=taxon:162145;gb-acronym=HMPV;gbkey=Src;genome=genomic;isolate=00-1;mol_type=genomic RNA | ||
| NC_039199.1 RefSeq CDS 40 1224 . + 0 Name=N;Dbxref=GenBank:YP_009513265.1,GeneID:37626968;gbkey=CDS;gene=N;locus_tag=D1Y22_gp1;product=nucleoprotein;protein_id=N | ||
| NC_039199.1 RefSeq CDS 1248 2132 . + 0 Name=P;Dbxref=GenBank:YP_009513266.1,GeneID:37626964;gbkey=CDS;gene=P;locus_tag=D1Y22_gp2;product=phosphoprotein;protein_id=P | ||
| NC_039199.1 RefSeq CDS 2165 2929 . + 0 Name=M;Dbxref=GenBank:YP_009513267.1,GeneID:37626965;gbkey=CDS;gene=M;locus_tag=D1Y22_gp3;product=matrix protein;protein_id=M | ||
| NC_039199.1 RefSeq CDS 3052 4671 . + 0 Name=F;Dbxref=GenBank:YP_009513268.1,GeneID:37626966;gbkey=CDS;gene=F;locus_tag=D1Y22_gp4;product=fusion protein;protein_id=F | ||
| NC_039199.1 RefSeq CDS 4737 5300 . + 0 Name=M2-1;Dbxref=GenBank:YP_009513269.1,GeneID:37626967;gbkey=CDS;gene=M2-1;locus_tag=D1Y22_gp5;product=matrix protein 2-1;protein_id=M2-1 | ||
| NC_039199.1 RefSeq CDS 5248 5463 . + 0 Name=M2-2;Dbxref=GenBank:YP_009513270.1,GeneID:37626967;gbkey=CDS;gene=M2-2;locus_tag=D1Y22_gp5;product=matrix protein 2-2;protein_id=M2-2 | ||
| NC_039199.1 RefSeq CDS 5494 6045 . + 0 Name=SH;Dbxref=GenBank:YP_009513271.1,GeneID:37626961;gbkey=CDS;gene=SH;locus_tag=D1Y22_gp6;product=small hydrophobic protein;protein_id=SH | ||
| NC_039199.1 RefSeq CDS 6247 6957 . + 0 Name=G;Dbxref=GenBank:YP_009513272.1,GeneID:37626962;gbkey=CDS;gene=G;locus_tag=D1Y22_gp7;product=attachment glycoprotein;protein_id=G | ||
| NC_039199.1 RefSeq CDS 7167 13184 . + 0 Name=L;Dbxref=GenBank:YP_009513273.1,GeneID:37626963;gbkey=CDS;gene=L;locus_tag=D1Y22_gp8;product=RNA-dependent RNA polymerase;protein_id=L | ||
| ### |
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| { | ||
| "schemaVersion": "3.0.0", | ||
| "alignmentParams": { | ||
| "penaltyGapOpen": 12, | ||
| "penaltyGapOpenInFrame": 16, | ||
| "penaltyGapOpenOutOfFrame": 18, | ||
| "penaltyGapExtend": 1, | ||
| "gapAlignmentSide": "left", | ||
| "excessBandwidth": 50, | ||
| "terminalBandwidth": 50, | ||
| "minSeedCover": 0.1, | ||
| "kmerLength": 5, | ||
| "kmerDistance": 7, | ||
| "minMatchLength": 20, | ||
| "allowedMismatches": 10, | ||
| "windowSize": 30 | ||
| }, | ||
| "compatibility": { | ||
| "cli": "3.0.0-alpha.0", | ||
| "web": "3.0.0-alpha.0" | ||
| }, | ||
| "experimental": true, | ||
| "defaultCds": "F", | ||
| "files": { | ||
| "examples": "sequences.fasta", | ||
| "genomeAnnotation": "genome_annotation.gff3", | ||
| "pathogenJson": "pathogen.json", | ||
| "reference": "reference.fasta", | ||
| "treeJson": "tree.json", | ||
| "readme": "README.md", | ||
| "changelog": "CHANGELOG.md" | ||
| }, | ||
| "qc": { | ||
| "privateMutations": { | ||
| "enabled": true, | ||
| "typical": 150, | ||
| "cutoff": 200, | ||
| "weightLabeledSubstitutions": 2, | ||
| "weightReversionSubstitutions": 1, | ||
| "weightUnlabeledSubstitutions": 1 | ||
| }, | ||
| "missingData": { | ||
| "enabled": false, | ||
| "missingDataThreshold": 3000, | ||
| "scoreBias": 500 | ||
| }, | ||
| "snpClusters": { | ||
| "enabled": false, | ||
| "windowSize": 100, | ||
| "clusterCutOff": 10, | ||
| "scoreWeight": 50 | ||
| }, | ||
| "mixedSites": { | ||
| "enabled": true, | ||
| "mixedSitesThreshold": 10 | ||
| }, | ||
| "frameShifts": { | ||
| "enabled": true, | ||
| "scoreWeight": 10, | ||
| "ignoreFrameShifts": [ | ||
| { | ||
| "codonRange": { | ||
| "begin": 206, | ||
| "end": 224 | ||
| }, | ||
| "cdsName": "G" | ||
| } | ||
| ] | ||
| }, | ||
| "stopCodons": { | ||
| "enabled": true, | ||
| "ignoredStopCodons": [], | ||
| "scoreWeight": 5 | ||
| } | ||
| }, | ||
| "shortcuts": [ | ||
| "hmpv" | ||
| ], | ||
| "attributes": { | ||
| "name": "Human Metapneumovirus", | ||
| "reference accession": "NC_039199", | ||
| "reference name": "hMPV/00-1" | ||
| }, | ||
| "geneOrderPreference": [ | ||
| "N", | ||
| "P", | ||
| "M", | ||
| "F", | ||
| "M2-1", | ||
| "M2-2", | ||
| "SH", | ||
| "G", | ||
| "L" | ||
| ] | ||
| } |
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| >NC_039199.1 Human metapneumovirus isolate 00-1, complete genome | ||
| GTATAAATTAGATTCCAAAAAAATATGGGACAAGTGAAAATGTCTCTTCAAGGGATTCACCTGAGTGATTTATCATACAAGCATGCTATATTAAAAGAGTCTCAGTACACAATAAAAAGAGATGTGGGTACAACAACTGCAGTGACACCCTCATCATTGCAACAAGAAATAACACTGTTGTGTGGAGAAATTCTGTATGCTAAACATGCTGACTACAAATATGCTGCAGAAATAGGAATACAATATATTAGCACAGCTTTAGGATCAGAGAGAGTGCAGCAGATTCTGAGGAACTCAGGCAGTGAAGTCCAAGTGGTCTTAACCAGAACGTACTCTCTGGGGAAAATTAAAAACAATAAAGGAGAAGATTTACAGATGTTAGACATACACGGGGTAGAGAAGAGCTGGGTAGAAGAGATAGACAAAGAAGCAAGGAAAACAATGGCAACCTTGCTTAAGGAATCATCAGGTAATATCCCACAAAATCAGAGGCCCTCAGCACCAGACACACCCATAATCTTATTATGTGTAGGTGCCTTAATATTCACTAAACTAGCATCAACCATAGAAGTGGGACTAGAGACCACAGTCAGAAGGGCTAACCGTGTACTAAGTGATGCACTCAAGAGATACCCTAGAATGGACATACCAAAGATTGCCAGATCCTTCTATGACTTATTTGAACAAAAAGTGTATCACAGAAGTTTGTTCATTGAGTATGGCAAAGCATTAGGCTCATCATCTACAGGCAGCAAAGCAGAAAGTCTATTTGTTAATATATTCATGCAAGCTTATGGGGCCGGTCAAACAATGCTAAGGTGGGGGGTCATTGCCAGGTCATCCAACAATATAATGTTAGGACATGTATCCGTCCAAGCTGAGTTAAAACAGGTCACAGAAGTCTATGACTTGGTGCGAGAAATGGGCCCTGAATCTGGACTTCTACATTTAAGGCAAAGCCCAAAAGCTGGACTGTTATCACTAGCCAACTGTCCCAACTTTGCAAGTGTTGTTCTCGGAAATGCCTCAGGCTTAGGCATAATCGGTATGTATCGAGGGAGAGTACCAAACACAGAATTATTTTCAGCAGCTGAAAGTTATGCCAAAAGTTTGAAAGAAAGCAATAAAATAAATTTCTCTTCATTAGGACTTACAGATGAAGAGAAAGAGGCTGCAGAACATTTCTTAAATGTGAGTGACGACAGTCAAAATGATTATGAGTAATTAAAAAAGTGGGACAAGTCAAAATGTCATTCCCTGAAGGAAAAGATATTCTTTTCATGGGTAATGAAGCAGCAAAATTAGCAGAAGCTTTCCAGAAATCATTAAGAAAACCAGGTCATAAAAGATCTCAATCTATTATAGGAGAAAAAGTGAATACTGTATCAGAAACATTGGAATTACCTACTATCAGTAGACCTGCAAAACCAACCATACCGTCAGAACCAAAGTTAGCATGGACAGATAAAGGTGGGGCAACCAAAACTGAAATAAAGCAAGCAATCAAAGTCATGGATCCCATTGAAGAAGAAGAGTCTACCGAGAAGAAGGTGCTACCCTCCAGTGATGGGAAAACCCCTGCAGAAAAGAAACTGAAACCATCAACTAACACCAAAAAGAAGGTTTCATTTACACCAAATGAACCAGGGAAATATACAAAGTTGGAAAAAGATGCTCTAGATTTGCTCTCAGATAATGAAGAAGAAGATGCAGAATCTTCAATCTTAACCTTTGAAGAAAGAGATACTTCATCATTAAGCATTGAGGCCAGATTGGAATCAATAGAGGAGAAATTAAGCATGATATTAGGGCTATTAAGAACACTCAACATTGCTACAGCAGGACCCACAGCAGCAAGAGATGGGATCAGAGATGCAATGATTGGCGTAAGAGAGGAATTAATAGCAGACATAATAAAGGAAGCTAAAGGGAAAGCAGCAGAAATGATGGAAGAGGAAATGAGTCAACGATCAAAAATAGGAAATGGTAGTGTAAAATTAACAGAAAAAGCAAAAGAGCTCAACAAAATTGTTGAAGATGAAAGCACAAGTGGAGAATCCGAAGAAGAAGAAGAACCAAAAGACACACAAGACAATAGTCAAGAAGATGACATTTACCAGTTAATTATGTAGTTTAATAAAAATAAACAATGGGACAAGTAAAAATGGAGTCCTACCTAGTAGACACCTATCAAGGCATTCCTTACACAGCAGCTGTTCAAGTTGATCTAATAGAAAAGGACCTGTTACCTGCAAGCCTAACAATATGGTTCCCTTTGTTTCAGGCCAACACACCACCAGCAGTGCTGCTCGATCAGCTAAAAACCCTGACAATAACCACTCTGTATGCTGCATCACAAAATGGTCCAATACTCAAAGTGAATGCATCAGCCCAAGGTGCAGCAATGTCTGTACTTCCCAAAAAATTTGAAGTCAATGCGACTGTAGCACTCGATGAATATAGCAAACTGGAATTTGACAAACTCACAGTCTGTGAAGTAAAAACAGTTTACTTAACAACCATGAAACCATACGGGATGGTATCAAAATTTGTGAGCTCAGCCAAATCAGTTGGCAAAAAAACACATGATCTAATCGCACTATGTGATTTTATGGATCTAGAAAAGAACACACCTGTTACAATACCAGCATTCATCAAATCAGTTTCAATCAAAGAGAGTGAGTCAGCTACTGTTGAAGCTGCTATAAGCAGTGAAGCAGACCAAGCTCTAACACAGGCCAAAATTGCACCTTATGCGGGATTAATTATGATCATGACTATGAACAATCCCAAAGGCATATTCAAAAAGCTTGGAGCTGGGACTCAAGTCATAGTAGAACTAGGAGCATATGTCCAGGCTGAAAGCATAAGCAAAATATGCAAGACTTGGAGCCATCAAGGGACAAGATATGTCTTGAAGTCCAGATAACAACCAAGCACCTTGGCCAAGAGCTACTAACCCTATCTCATAGATCATAAAGTCACCATTCTAGTTATATAAAAATCAAGTTAGAACAAGAATTAAATCAATCAAGAACGGGACAAATAAAAATGTCTTGGAAAGTGGTGATCATTTTTTCATTGTTAATAACACCTCAACACGGTCTTAAAGAGAGCTACTTAGAAGAGTCATGTAGCACTATAACTGAAGGATATCTCAGTGTTCTGAGGACAGGTTGGTACACCAATGTTTTTACACTGGAGGTAGGCGATGTAGAGAACCTTACATGTGCCGATGGACCCAGCTTAATAAAAACAGAATTAGACCTGACCAAAAGTGCACTAAGAGAGCTCAGAACAGTTTCTGCTGATCAACTGGCAAGAGAGGAGCAAATTGAAAATCCCAGACAATCTAGATTCGTTCTAGGAGCAATAGCACTCGGTGTTGCAACTGCAGCTGCAGTTACAGCAGGTGTTGCAATTGCCAAAACCATCCGGCTTGAAAGTGAAGTAACAGCAATTAAGAATGCCCTCAAAAAGACCAATGAAGCAGTATCTACATTGGGGAATGGAGTTCGTGTGTTGGCAACTGCAGTGAGAGAGCTGAAAGATTTTGTGAGCAAGAATCTAACACGTGCAATCAACAAAAACAAGTGCGACATTGCTGACCTGAAAATGGCCGTTAGCTTCAGTCAATTCAACAGAAGGTTCCTAAATGTTGTGCGGCAATTTTCAGACAACGCTGGAATAACACCAGCAATATCTTTGGACTTAATGACAGATGCTGAACTAGCCAGAGCTGTTTCCAACATGCCAACATCTGCAGGACAAATAAAACTGATGTTGGAGAACCGTGCAATGGTAAGAAGAAAAGGGTTCGGATTCCTGATAGGAGTTTACGGAAGCTCCGTAATTTACATGGTGCAACTGCCAATCTTTGGGGTTATAGACACGCCTTGCTGGATAGTAAAAGCAGCCCCTTCTTGTTCAGGAAAAAAGGGAAACTATGCTTGCCTCTTAAGAGAAGACCAAGGATGGTATTGTCAAAATGCAGGGTCAACTGTTTACTACCCAAATGAAAAAGACTGTGAAACAAGAGGAGACCATGTCTTTTGCGACACAGCAGCAGGAATCAATGTTGCTGAGCAGTCAAAGGAGTGCAACATAAACATATCTACTACTAATTACCCATGCAAAGTTAGCACAGGAAGACATCCTATCAGTATGGTTGCACTATCTCCTCTTGGGGCTTTGGTTGCTTGCTACAAGGGAGTGAGCTGTTCCATTGGCAGCAACAGAGTAGGGATCATCAAGCAACTGAACAAAGGCTGCTCTTATATAACCAACCAAGACGCAGACACAGTGACAATAGACAACACTGTATACCAGCTAAGCAAAGTTGAAGGCGAACAGCATGTTATAAAAGGAAGGCCAGTGTCAAGCAGCTTTGACCCAGTCAAGTTTCCTGAAGATCAATTCAATGTTGCACTTGACCAAGTTTTCGAGAGCATTGAGAACAGTCAGGCCTTGGTGGATCAATCAAACAGAATCCTAAGCAGTGCAGAGAAAGGAAACACTGGCTTCATCATTGTAATAATTCTAATTGCTGTCCTTGGCTCTACCATGATCCTAGTGAGTGTTTTTATCATAATAAAGAAAACAAAGAAACCCACAGGAGCACCTCCAGAGCTGAGTGGTGTCACAAACAATGGCTTCATACCACATAATTAGTTAATTAAAAATAAAGTAAATTAAAATAAATTAAAATTAAAAATAAAAATTTGGGACAAATCATAATGTCTCGCAAGGCTCCGTGCAAATATGAAGTGCGGGGCAAATGCAATAGAGGAAGTGAGTGCAAGTTTAACCACAATTACTGGAGTTGGCCAGATAGATACTTATTAATAAGATCAAATTATTTATTAAATCAACTTTTAAGGAACACTGATAGAGCTGATGGCTTATCAATAATATCAGGAGCAGGCAGAGAAGATAGGACACAAGATTTTGTCCTAGGTTCCACCAATGTGGTTCAAGGTTATATTGATGATAACCAAAGCATAACAAAAGCTGCAGCCTGTTACAGTCTACATAATATAATCAAACAACTACAAGAAGTTGAAGTTAGGCAGGCTAGAGATAACAAACTATCTGACAGCAAACATGTAGCACTTCACAACTTAGTCCTATCTTATATGGAGATGAGCAAAACTCCTGCATCTTTAATCAACAATCTCAAGAGACTGCCGAGAGAGAAACTGAAAAAATTAGCAAAGCTCATAATTGACTTATCAGCAGGTGCTGAAAATGACTCTTCATATGCCTTGCAAGACAGTGAAAGCACTAATCAAGTGCAGTGAGCATGGTCCAGTTTTCATTACTATAGAGGTTGATGACATGATATGGACTCACAAGGACTTAAAAGAAGCTTTATCTGATGGGATAGTGAAGTCTCATACTAACATTTACAATTGTTATTTAGAAAACATAGAAATTATATATGTCAAGGCTTACTTAAGTTAGTAAAAACACATCAGAGTGGGATAAATGACAATGATAACATTAGATGTCATTAAAAGTGATGGGTCTTCAAAAACATGTACTCACCTCAAAAAAATAATTAAAGACCACTCTGGTAAAGTGCTTATTGTACTTAAGTTAATATTAGCTTTACTAACATTTCTCACAGTAACAATCACCATCAATTATATAAAAGTGGAAAACAATCTGCAAATATGCCAGTCAAAAACTGAATCAGACAAAAAGGACTCATCATCAAATACCACATCAGTCACAACCAAGACTACTCTAAATCATGATATCACACAGTATTTTAAAAGTTTGATTCAAAGGTATACAAACTCTGCAATAAACAGTGACACATGCTGGAAAATAAACAGAAATCAATGCACAAATATAACAACATACAAATTTTTATGTTTTAAATCTGAAGACACAAAAACCAACAATTGTGATAAACTGACAGATTTATGCAGAAACAAACCAAAACCAGCAGTTGGAGTGTATCACATAGTAGAATGCCATTGTATATACACAGTTAAATGGAAGTGCTATCATTACCCAACCGATGAAACCCAATCCTAAATGTTAACACCAGATTAGGATCCATCCAAGTCTGTTAGTTCAACAATTTAGTTATTTAAAAATATTTTGAAAACAAGTAAGTTTCTATGATACTTCATAATAATAAGTAATAATTAATTGCTTAATCATCATCACAACATTATTCGAAACCATAACTATTCAATTTAAAAAGTAAAAAACAATAACATGGGACAAGTAGTTATGGAGGTGAAAGTGGAGAACATTCGAACAATAGATATGCTCAAAGCAAGAGTAAAAAATCGTGTGGCACGCAGCAAATGCTTTAAAAATGCCTCTTTGGTCCTCATAGGAATAACTACATTGAGTATTGCCCTCAATATCTATCTGATCATAAACTATAAAATGCAAAAAAACACATCTGAATCAGAACATCACACCAGCTCATCACCCATGGAATCCAGCAGAGAAACTCCAACGGTCCCCACAGACAACTCAGACACCAACTCAAGCCCACAGCATCCAACTCAACAGTCCACAGAAGGCTCCACACTCTACTTTGCAGCCTCAGCAAGCTCACCAGAGACAGAACCAACATCAACACCAGATACAACAAACCGCCCGCCCTTCGTCGACACACACACAACACCACCAAGCGCAAGCAGAACAAAGACAAGTCCGGCAGTCCACACAAAAAACAACCCAAGGACAAGCTCTAGAACACATTCTCCACCACGGGCAACGACAAGGACGGCACGCAGAACCACCACTCTCCGCACAAGCAGCACAAGAAAGAGACCGTCCACAGCATCAGTCCAACCTGACATCAGCGCAACAACCCACAAAAACGAAGAAGCAAGTCCAGCGAGCCCACAAACATCTGCAAGCACAACAAGAATACAAAGGAAAAGCGTGGAGGCCAACACATCAACAACATACAACCAAACTAGTTAACAAAAAATACAAAATAACTCTAAGATAAACCATGCAGACACCAACAATGGAGAAGCCAAAAGACAATTCACAATCTCCCCAAAAAGGCAACAACACCATATTAGCTCTGCCCAAATCTCCCTGGAAAAAACACTCGCCCATATACCAAAAATACCACAACCACCCCAAGAAAAAAACTGGGCAAAACAACACCCAAGAGACAAATAACAATGGATCCTCTCAATGAATCCACTGTTAATGTCTATCTTCCTGACTCATATCTTAAAGGAGTGATTTCCTTTAGTGAGACTAATGCAATTGGTTCATGTCTCTTAAAAAGACCTTACCTAAAAAATGACAACACTGCAAAAGTTGCCATAGAGAATCCTGTTATCGAGCATGTTAGACTCAAAAATGCAGTCAATTCTAAGATGAAAATATCAGATTACAAGATAGTAGAGCCAGTAAACATGCAACATGAAATTATGAAGAATGTACACAGTTGTGAGCTCACATTATTAAAACAGTTTTTAACAAGGAGTAAAAATATTAGCACTCTCAAATTAAATATGATATGTGATTGGCTGCAGTTAAAGTCTACATCAGATGATACCTCAATCCTAAGTTTTATAGATGTAGAATTTATACCTAGCTGGGTAAGCAATTGGTTTAGTAATTGGTACAATCTCAACAAGTTGATTCTGGAATTCAGGAAAGAAGAAGTAATAAGAACTGGTTCAATCTTGTGTAGGTCATTGGGTAAATTAGTTTTTGTTGTATCATCATATGGATGTATAGTCAAGAGCAACAAAAGCAAAAGAGTGAGCTTCTTCACATACAATCAACTGTTAACATGGAAAGATGTGATGTTAAGTAGATTCAATGCAAATTTTTGTATATGGGTAAGCAACAGTCTGAATGAAAATCAAGAAGGGCTAGGGTTGAGAAGTAATCTGCAAGGCATATTAACTAATAAGCTATATGAAACTGTAGATTATATGCTTAGTTTATGTTGCAATGAAGGTTTCTCACTTGTGAAAGAGTTCGAAGGCTTTATTATGAGTGAAATTCTTAGGATTACTGAACATGCTCAATTCAGTACTAGATTTAGAAATACTTTATTAAATGGATTAACTGATCAATTAACAAAATTAAAAAATAAAAACAGACTCAGAGTTCATGGTACCGTGTTAGAAAATAATGATTATCCAATGTACGAAGTTGTACTTAAGTTATTAGGAGATACTTTGAGATGTATTAAATTATTAATCAATAAAAACTTAGAGAATGCTGCTGAATTATACTATATATTTAGAATATTCGGTCACCCAATGGTAGATGAAAGAGATGCAATGGATGCTGTCAAATTAAACAATGAAATCACAAAAATCCTTAGGTGGGAGAGCTTGACAGAACTAAGAGGGGCATTCATATTAAGGATTATCAAAGGATTTGTAGACAACAACAAAAGATGGCCCAAAATTAAAAACTTAAAAGTGCTTAGTAAGAGATGGACTATGTACTTCAAAGCAAAAAGTTACCCCAGTCAACTTGAATTAAGCGAACAAGATTTTTTAGAGCTTGCTGCAATACAGTTTGAACAAGAGTTTTCTGTCCCTGAAAAAACCAACCTTGAGATGGTATTAAATGATAAAGCTATATCACCTCCTAAAAGATTAATATGGTCTGTGTATCCAAAAAATTACTTACCTGAGAAAATAAAAAATCGATATCTAGAAGAGACTTTCAATGCAAGTGATAGTCTCAAAACAAGAAGAGTACTAGAGTACTATTTGAAAGATAATAAATTCGACCAAAAAGAACTTAAAAGTTATGTTGTTAAACAAGAATATTTAAATGATAAGGATCATATTGTCTCGCTAACTGGAAAAGAAAGAGAATTAAGTGTAGGTAGAATGTTTGCTATGCAACCAGGAAAACAGCGACAAATACAAATATTGGCTGAAAAATTGTTAGCTGATAATATTGTACCTTTTTTCCCAGAAACCTTAACAAAGTATGGTGATCTAGATCTTCAGAGAATAATGGAAATCAAATCGGAACTTTCTTCTATTAAAACTAGAAGAAATGATAGTTATAATAATTACATTGCAAGAGCATCCATAGTAACAGATTTAAGTAAGTTCAACCAAGCCTTTAGGTATGAAACTACAGCGATCTGTGCGGATGTAGCAGATGAACTACATGGAACACAAAGCCTATTCTGTTGGTTACATCTTATCGTCCCTATGACAACAATGATATGTGCCTATAGACATGCACCACCAGAAACAAAAGGTGAATATGATATAGATAAGATAGAAGAGCAAAGTGGTTTATATAGATATCATATGGGTGGTATTGAAGGATGGTGTCAAAAACTCTGGACAATGGAAGCTATATCTCTATTAGATGTTGTATCTGTAAAAACACGATGTCAAATGACATCTTTATTAAACGGTGACAACCAATCAATAGATGTAAGTAAACCAGTTAAGTTATCTGAGGGTTTAGATGAAGTGAAAGCAGATTATAGCTTGGCTGTAAAAATGTTAAAAGAAATAAGAGATGCATACAGAAATATAGGCCATAAACTTAAAGAAGGGGAAACATATATATCAAGAGATCTTCAGTTTATAAGTAAGGTGATTCAATCTGAAGGAGTAATGCATCCTACCCCTATAAAAAAGATCTTAAGAGTGGGACCATGGATAAACACAATATTAGATGACATTAAAACCAGTGCAGAGTCAATAGGGAGTCTATGTCAGGAATTAGAATTTAGGGGGGAAAGCATAATAGTTAGTCTGATATTAAGGAATTTTTGGCTGTATAATTTATACATGCATGAATCAAAGCAACACCCCCTAGCAGGGAAGCAGTTATTCAAACAACTAAATAAAACATTAACATCAGTGCAGAGATTTTTTGAAATAAAAAAGGAAAATGAAGTAGTAGATCTATGGATGAACATACCAATGCAGTTTGGAGGAGGAGATCCAGTAGTCTTCTATAGATCTTTCTATAGAAGGACCCCTGATTTTTTAACTGAAGCAATCAGTCATGTGGATATTCTGTTAAGAATATCAGCCAACATAAGAAATGAAGCGAAAATAAGTTTCTTCAAAGCCTTACTGTCAATAGAAAAAAATGAACGTGCTACACTGACAACACTAATGAGAGATCCTCAAGCTGTTGGCTCAGAGCGACAAGCAAAAGTAACAAGTGATATCAATAGAACAGCAGTTACCAGCATCTTAAGTCTTTCTCCAAATCAACTTTTCAGCGATAGTGCTATACACTACAGTAGAAATGAAGAAGAGGTCGGAATCATTGCTGACAACATAACACCTGTTTATCCTCATGGACTGAGAGTTTTGTATGAATCATTACCTTTTCATAAAGCTGAAAAAGTTGTGAATATGATATCAGGAACGAAATCCATAACCAACTTATTACAGAGAACATCTGCTATTAATGGTGAAGATATTGACAGAGCTGTATCCATGATGCTGGAGAACCTAGGATTATTATCTAGAATATTGTCAGTAGTTGTTGATAGTATAGAAATTCCAACCAAATCTAATGGTAGGCTGATATGTTGTCAGATATCTAGAACCCTAAGGGAGACATCATGGAATAATATGGAAATAGTTGGAGTAACATCCCCTAGCATCACTACATGCATGGATGTCATATATGCAACTAGCTCTCATTTGAAAGGGATAATCATTGAAAAGTTCAGCACTGACAGAACTACAAGAGGTCAAAGAGGTCCAAAGAGCCCTTGGGTAGGGTCGAGCACTCAAGAGAAAAAATTAGTTCCTGTTTATAACAGACAAATTCTTTCAAAACAACAAAGAGAACAGCTAGAAGCAATTGGAAAAATGAGATGGGTATATAAAGGGACACCAGGTTTAAGACGATTACTCAATAAGATTTGTCTTGGAAGTTTAGGCATTAGTTACAAATGTGTAAAACCTTTATTACCTAGGTTTATGAGTGTAAATTTCCTACACAGGTTATCTGTCAGTAGTAGACCTATGGAATTCCCAGCATCAGTTCCAGCTTATAGAACAACAAATTACCATTTTGACACTAGTCCTATTAATCAAGCACTAAGTGAGAGATTTGGGAATGAAGATATTAATTTGGTCTTCCAAAATGCAATCAGCTGTGGAATTAGCATAATGAGTGTAGTAGAACAATTAACTGGTAGGAGTCCAAAACAGTTAGTTTTAATACCTCAATTAGAAGAAATAGACATTATGCCACCACCAGTGTTTCAAGGGAAATTCAATTATAAGCTAGTAGATAAGATAACTTCTGATCAACATATCTTCAGTCCAGACAAAATAGATATGTTAACACTGGGGAAAATGCTCATGCCCACTATAAAAGGTCAGAAAACAGATCAGTTCCTGAACAAGAGAGAGAATTATTTCCATGGGAATAATCTTATTGAGTCTTTGTCAGCAGCGTTAGCATGTCATTGGTGTGGGATATTAACAGAGCAATGTATAGAAAATAATATTTTCAAGAAAGACTGGGGTGACGGGTTCATATCGGATCATGCTTTTATGGACTTCAAAATATTCCTATGTGTCTTTAAAACTAAACTTTTATGTAGTTGGGGGTCCCAAGGGAAAAACATTAAAGATGAAGATATAGTAGATGAATCAATAGATAAACTGTTAAGGATTGATAATACTTTTTGGAGAATGTTCAGCAAGGTTATGTTTGAATCAAAGGTTAAGAAAAGGATAATGTTATATGATGTAAAATTTCTATCATTAGTAGGTTATATAGGGTTTAAGAATTGGTTTATAGAACAGTTGAGATCAGCTGAGTTGCATGAGGTACCTTGGATTGTCAATGCCGAAGGTGATCTGGTTGAGATCAAGTCAATTAAAATCTATTTGCAACTGATAGAGCAAAGTTTATTTTTAAGAATAACTGTTTTGAACTATACAGATATGGCACATGCTCTCACAAGATTAATCAGAAAGAAGTTGATGTGTGATAATGCACTATTAACTCCGATTCCATCCCCAATGGTTAATTTAACTCAAGTTATTGATCCTACAGAACAATTAGCTTATTTCCCTAAGATAACATTTGAAAGGCTAAAAAATTATGACACTAGTTCAAATTATGCTAAAGGAAAGCTAACAAGGAATTACATGATACTGTTGCCATGGCAACATGTTAATAGATATAACTTTGTCTTTAGTTCTACTGGATGTAAAGTTAGTCTAAAAACATGCATTGGAAAACTTATGAAAGATCTAAACCCTAAAGTTCTGTACTTTATTGGAGAAGGGGCAGGAAATTGGATGGCCAGAACAGCATGTGAATATCCTGACATCAAATTTGTATACAGAAGTTTAAAAGATGACCTTGATCATCATTATCCTTTGGAATACCAGAGAGTTATAGGAGAATTAAGCAGGATAATAGATAGCGGTGAAGGGCTTTCAATGGAAACAACAGATGCAACTCAAAAAACTCATTGGGATTTGATACACAGAGTAAGCAAAGATGCTTTATTAATAACTTTATGTGATGCAGAATTTAAGGACAGAGATGATTTTTTTAAGATGGTAATTCTATGGAGGAAACATGTATTATCATGCAGAATTTGCACTACTTATGGGACAGACCTCTATTTATTCGCAAAGTATCATGCTAAAGACTGCAATGTAAAATTACCTTTTTTTGTGAGATCAGTAGCCACCTTTATTATGCAAGGTAGTAAACTGTCAGGCTCAGAATGCTACATACTCTTAACACTAGGCCACCACAACAATTTACCCTGCCATGGAGAAATACAAAATTCTAAGATGAAAATAGCAGTGTGTAATGATTTTTATGCTGCAAAAAAACTTGACAATAAATCTATTGAAGCCAACTGTAAATCACTTTTATCAGGGCTAAGAATACCGATAAATAAGAAAGAATTAAATAGACAGAGAAGGTTATTAACACTACAAAGCAACCATTCTTCTGTAGCAACAGTTGGAGGTAGCAAGGTCATAGAGTCTAAATGGTTAACAAACAAGGCAAACACAATAATTGATTGGTTAGAACATATTTTAAATTCTCCAAAAGGTGAATTAAATTATGATTTTTTTGAAGCATTAGAAAATACTTACCCTAATATGATTAAACTAATAGATAATCTAGGGAATGCAGAGATAAAAAAACTGATCAAAGTAACTGGATATATGCTTGTAAGTAAAAAATGAAAAATGATAAAAATGATAAAATAGGTGACAACTTCATACTATTCCAAAGTAATCATTTGATTATGCAATTATGTAATAGTTAATTAAAAACTAAAAATCAAAAGTTAGAAACTAACAACTGTCATTAAGTTTATTAAAAATAAGAAATTATAATTGGATGTATACG 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