Add Dengue virus DENVx genotypes dataset

data/nextstrain/collection.json

@@ -54,6 +54,10 @@
     "nextstrain/flu/h3n2/pb2",
     "nextstrain/measles",
     "nextstrain/measles/N450/WHO-2012",
-    "nextstrain/dengue/all"
+    "nextstrain/dengue/all",
+    "nextstrain/dengue/denv1",
+    "nextstrain/dengue/denv4",
+    "nextstrain/dengue/denv2",
+    "nextstrain/dengue/denv3"
   ]
 }

data/nextstrain/dengue/README.md

@@ -0,0 +1,8 @@
+# Nextclade v3 Dataset
+
+| Serotype | Reference | Nextclade link |
+|:--|:--|:--|
+| denv1 | [NC_001477.1](https://www.ncbi.nlm.nih.gov/nuccore/NC_001477.1) | [nextclade denv1](https://clades.nextstrain.org/?dataset-url=https://github.com/nextstrain/dengue/tree/main/nextclade_data/denv1) |
+| denv2 | [NC_001474.2](https://www.ncbi.nlm.nih.gov/nuccore/NC_001474.2) | [nextclade denv2](https://clades.nextstrain.org/?dataset-url=https://github.com/nextstrain/dengue/tree/main/nextclade_data/denv2) |
+| denv3 | [NC_001475.2](https://www.ncbi.nlm.nih.gov/nuccore/NC_001475.2) | [nextclade denv3](https://clades.nextstrain.org/?dataset-url=https://github.com/nextstrain/dengue/tree/main/nextclade_data/denv3) |
+| denv4 | [NC_002640.1](https://www.ncbi.nlm.nih.gov/nuccore/NC_002640.1) | [nextclade denv4](https://clades.nextstrain.org/?dataset-url=https://github.com/nextstrain/dengue/tree/main/nextclade_data/denv4) |

data/nextstrain/dengue/denv1/CHANGELOG.md

@@ -0,0 +1,5 @@
+## Unreleased
+
+Initial release for Nextclade v3!
+
+Read more about Nextclade datasets in the documentation: https://docs.nextstrain.org/projects/nextclade/en/stable/user/datasets.html

data/nextstrain/dengue/denv1/README.md

@@ -0,0 +1,25 @@
+# Dengue virus DENV1 dataset
+
+| Key  | Value  |
+| :-- | :-- |
+| name  | Dengue virus DENV1 genotypes |
+| authors | [Nextstrain](https://nextstrain.org) |
+| reference | NC_001477.1 |
+| workflow  | https://github.com/nextstrain/dengue/tree/main/nextclade  |
+| path  | `nextstrain/dengue/denv1` |
+
+## Scope of this dataset
+
+This dataset assigns DENV1 genotypes to dengue serotype 1 samples based on [curated reference strains by Sidney Bell](https://github.com/blab/dengue-antigenic-dynamics/blob/master/data/reference/strain_genotypes.tsv) from [Bell et al, 2019](https://elifesciences.org/articles/42496).
+
+## Features
+
+This dataset supports:
+
+- Assignment of serotypes
+- Phylogenetic placement
+- Sequence quality control (QC)
+
+## What are Nextclade datasets
+
+Read more about Nextclade datasets in the Nextclade documentation: https://docs.nextstrain.org/projects/nextclade/en/stable/user/datasets.html

data/nextstrain/dengue/denv1/genome_annotation.gff3

@@ -0,0 +1,14 @@
+##gff-version 3
+##sequence-region Reconstructed_root_sequence_of_https_nextstrain_org_dengue/denv1/genome 1 10735
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/denv1/genome	feature	gene	95	436	.	+	.	codon_start=1;gene=C;gene_name=C;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/denv1/genome	feature	gene	437	709	.	+	.	codon_start=1;gene=pr;gene_name=pr;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/denv1/genome	feature	gene	437	934	.	+	.	codon_start=1;gene=M;gene_name=M;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/denv1/genome	feature	gene	935	2419	.	+	.	codon_start=1;gene=E;gene_name=E;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/denv1/genome	feature	gene	2420	3475	.	+	.	codon_start=1;gene=NS1;gene_name=NS1;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/denv1/genome	feature	gene	3476	4129	.	+	.	codon_start=1;gene=NS2A;gene_name=NS2A;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/denv1/genome	feature	gene	4130	4519	.	+	.	codon_start=1;gene=NS2B;gene_name=NS2B;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/denv1/genome	feature	gene	4520	6376	.	+	.	codon_start=1;gene=NS3;gene_name=NS3;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/denv1/genome	feature	gene	6377	6757	.	+	.	codon_start=1;gene=NS4A;gene_name=NS4A;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/denv1/genome	feature	gene	6758	6826	.	+	.	codon_start=1;gene=2K;gene_name=2K;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/denv1/genome	feature	gene	6827	7573	.	+	.	codon_start=1;gene=NS4B;gene_name=NS4B;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/denv1/genome	feature	gene	7574	10270	.	+	.	codon_start=1;gene=NS5;gene_name=NS5;

data/nextstrain/dengue/denv1/pathogen.json

@@ -0,0 +1,71 @@
+{
+  "alignmentParams": {
+    "penaltyGapOpen": 8,
+    "penaltyGapOpenInFrame": 12,
+    "penaltyGapOpenOutOfFrame": 14,
+    "gapAlignmentSide": "left",
+    "minSeedCover": 0.1,
+    "minLength": 1000
+  },
+  "attributes": {
+    "name": "Dengue virus DENV1 genotypes",
+    "reference accession": "NC_001477",
+    "reference name": "NC_001477"
+  },
+  "compatibility": {
+    "cli": "3.0.0-alpha.0",
+    "web": "3.0.0-alpha.0"
+  },
+  "deprecated": false,
+  "enabled": true,
+  "experimental": true,
+  "files": {
+    "changelog": "CHANGELOG.md",
+    "examples": "sequences.fasta",
+    "genomeAnnotation": "genome_annotation.gff3",
+    "pathogenJson": "pathogen.json",
+    "readme": "README.md",
+    "reference": "reference.fasta",
+    "treeJson": "tree.json"
+  },
+  "meta": {
+    "bugs": "https://github.com/nextstrain/nextclade_data/issues",
+    "source code": "https://github.com/nextstrain/nextclade_data"
+  },
+  "qc": {
+    "frameShifts": {
+      "enabled": true
+    },
+    "missingData": {
+      "enabled": false,
+      "missingDataThreshold": 1000,
+      "scoreBias": 100
+    },
+    "mixedSites": {
+      "enabled": false,
+      "mixedSitesThreshold": 8
+    },
+    "privateMutations": {
+      "cutoff": 25,
+      "enabled": false,
+      "typical": 10,
+      "weightLabeledSubstitutions": 2,
+      "weightReversionSubstitutions": 1,
+      "weightUnlabeledSubstitutions": 1
+    },
+    "snpClusters": {
+      "clusterCutOff": 5,
+      "enabled": false,
+      "scoreWeight": 50,
+      "windowSize": 100
+    },
+    "stopCodons": {
+      "enabled": true
+    }
+  },
+  "schemaVersion": "3.0.0",
+  "version": {
+    "tag": "unreleased"
+  },
+  "defaultCds": "E"
+}

data/nextstrain/dengue/denv1/reference.fasta

@@ -0,0 +1,2 @@
+>Reconstructed_root_sequence_of_https_nextstrain_org_dengue/denv1/genome
+AGTTGTTAGTCTACGTGGACCGACAAGAACAGTTTCGAATCGGAAGCTTGCTTAACGTAGTTCTAACAGTTTTTTATTAGAGAGCAGATCTCTGATGAACAACCAACGGAAAAAGACGGGTCGACCGTCTTTCAATATGCTGAAACGCGCGAGAAACCGCGTGTCAACTGTTTCACAGTTGGCGAAGAGATTCTCAAAAGGATTGCTCTCAGGCCAAGGACCCATGAAATTGGTGATGGCTTTCATAGCATTCCTAAGATTTCTAGCCATACCCCCAACAGCAGGAATTTTGGCTAGATGGGGCTCATTCAAGAAGAATGGAGCGATTAAAGTGTTACGGGGTTTCAAGAAAGAAATCTCAAACATGTTGAACATAATGAACAGGAGGAAAAGATCCGTGACCATGCTCCTTATGCTGCTGCCCACAGCCCTGGCGTTCCATCTGACCACACGAGGGGGAGAGCCGCACATGATAGTTAGCAAGCAGGAAAGAGGAAAGTCACTTTTGTTTAAGACCTCTGCAGGTGTCAACATGTGCACCCTTATTGCGATGGATTTGGGAGAGTTATGTGAGGACACAATGACCTACAAATGCCCCCGGATCACTGAGGCGGAACCAGATGACGTTGACTGTTGGTGCAATGCCACGGACACATGGGTGACCTATGGAACGTGTTCTCAAACTGGCGAACACCGACGAGACAAACGTTCCGTCGCACTGGCCCCACACGTGGGGCTTGGTCTAGAAACAAGAACCGAAACGTGGATGTCCTCTGAAGGCGCTTGGAAACAAATACAAAAAGTGGAGACTTGGGCTCTGAGACACCCAGGATTCACGGTGATAGCCCTTTTTCTAGCACATGCCATAGGAACATCCATCACCCAGAAAGGGATTATTTTCATTTTGCTGATGCTGGTAACACCATCCATGGCCATGCGATGCGTGGGAATAGGCAACAGAGACTTCGTGGAAGGACTGTCAGGAGCAACGTGGGTGGATGTGGTACTGGAGCATGGAAGTTGCGTCACCACCATGGCAAAAAATAAACCAACATTGGACATTGAACTCTTGAAGACGGAGGTCACAAACCCTGCCGTCCTGCGCAAACTGTGCATTGAAGCTAAAATATCAAACACCACCACCGATTCAAGATGTCCAACACAAGGAGAAGCCACACTGGTGGAAGAACAAGACGCGAACTTTGTGTGTCGACGAACGTTCGTGGACAGAGGCTGGGGCAATGGCTGTGGGCTATTCGGAAAAGGTAGCCTAATAACGTGTGCTAAGTTCAAGTGTGTGACAAAACTAGAAGGAAAGATAGTTCAATATGAAAACTTAAAATATTCAGTGATAGTCACCGTCCACACTGGAGACCAGCACCAGGTGGGAAATGAGACCACAGAACATGGAACAACTGCAACCATAACACCTCAAGCTCCTACGTCGGAAATACAGCTGACCGACTACGGAGCCCTTACATTGGATTGCTCACCTAGAACAGGGCTAGACTTTAATGAGATGGTGTTGTTGACAATGAAAGAAAAATCATGGCTTGTCCACAAACAATGGTTTCTAGACTTACCACTGCCTTGGACCTCGGGGGCTTCAACATCCCAAGAGACTTGGAACAGACAAGATTTGCTGGTCACATTTAAGACAGCTCATGCAAAGAAGCAGGAAGTAGTCGTACTAGGATCACAAGAAGGAGCAATGCACACTGCGTTGACTGGAGCGACAGAAATCCAAACGTCAGGAACGACAACAATTTTTGCAGGACACCTGAAATGCAGACTAAAAATGGACAAACTGACTTTAAAAGGGATGTCATATGTGATGTGCACAGGCTCATTCAAGTTAGAGAAAGAAGTGGCTGAGACCCAGCATGGAACTGTTCTAGTGCAGGTTAAATACGAAGGAACAGATGCACCATGCAAGATCCCCTTTTCGACCCAAGATGAGAAAGGAGTAACCCAGAATGGGAGATTGATAACAGCCAACCCCATAGTCACTGACAAAGAAAAACCAGTCAACATTGAGGCAGAACCACCTTTTGGTGAGAGCTACATCGTGGTAGGAGCAGGTGAAAAAGCTTTGAAACTAAGCTGGTTCAAGAAAGGAAGCAGCATAGGGAAAATGTTTGAAGCAACTGCCCGAGGAGCACGAAGGATGGCCATCCTGGGAGACACCGCATGGGACTTCGGTTCTATAGGAGGAGTGTTCACGTCTGTGGGAAAACTGGTACACCAGGTTTTTGGAACTGCATATGGAGTTTTGTTCAGCGGTGTTTCTTGGACCATGAAAATAGGAATAGGGATTCTGCTGACATGGCTAGGATTAAATTCAAGGAGCACGTCCCTTTCGATGACGTGCATTGCAGTTGGCATGGTCACACTGTACCTAGGAGTCATGGTTCAGGCGGACTCGGGATGTGTAATCAACTGGAAAGGCAGAGAACTCAAATGTGGAAGTGGCATTTTTGTCACTAATGAAGTTCACACTTGGACAGAGCAATACAAATTCCAGGCTGACTCCCCAAAGAGACTATCAGCAGCCATTGGGAAGGCATGGGAGGAGGGTGTGTGTGGAATTCGATCAGCCACTCGTCTCGAGAACATCATGTGGAAGCAAATATCAAATGAATTGAACCACATCCTACTTGAAAATGACATGAAATTCACAGTGGTCGTAGGAGATGTTAGTGGAATCTTGGCCCAAGGAAAAAAAATGATTAGGCCACAACCCATGGAACACAAATACTCGTGGAAAAGCTGGGGAAAAGCCAAAATCATAGGAGCAGATGTACAGAACACCACCTTCATCATCGACGGCCCAAACACCCCAGAATGCCCTGATGACCAAAGAGCATGGAACATTTGGGAAGTTGAGGACTATGGATTTGGAATTTTCACGACAAACATATGGTTGAAATTGCGTGACTCCTACACCCAAGTGTGTGACCACCGGCTAATGTCAGCTGCCATCAAGGACAGCAAGGCAGTCCATGCTGACATGGGGTACTGGATAGAAAGTGAAAAGAACGAGACCTGGAAGCTGGCGAGAGCCTCCTTCATAGAAGTTAAGACATGCATCTGGCCAAAATCCCACACTCTATGGAGCAATGGAGTTCTGGAAAGTGAAATGATAATCCCAAAGATATATGGAGGACCAATATCTCAGCACAACTACAGACCAGGATATTTCACACAAACAGCAGGGCCGTGGCACCTAGGCAAGTTGGAACTAGATTTTGATTTGTGTGAAGGTACCACAGTTGTTGTGGATGAACATTGTGGAAATCGAGGACCATCTCTTAGAACCACAACAGTCACAGGAAAGATAATCCATGAATGGTGCTGCAGATCTTGTACGCTACCCCCCTTACGTTTCAAAGGAGAAGACGGGTGTTGGTACGGCATGGAAATCAGACCAGTCAAGGAGAAGGAAGAGAACCTAGTCAAGTCAATGGTCTCTGCAGGGTCAGGAGAAGTGGACAGCTTTTCACTAGGACTGCTATGCATATCAATAATGATTGAAGAGGTGATGAGATCCAGATGGAGTAGAAAAATGCTGATGACTGGAACACTGGCTGTGTTCCTCCTTCTTATAATGGGACAATTGACATGGAATGATCTGATCAGGTTATGCATCATGGTTGGAGCCAATGCTTCAGACAGGATGGGGATGGGAACAACGTACCTAGCTTTGATGGCCACTTTCAAAATGAGACCAATGTTCGCTGTCGGGCTATTATTTCGCAGACTAACATCTAGAGAAGTTCTTCTTCTTACAATTGGATTGAGCCTGGTGGCATCCGTGGAGCTACCAAATTCCTTAGAGGAGCTGGGGGATGGACTTGCAATGGGCATCATGATGTTAAAATTACTGACTGATTTTCAGTCACACCAGCTATGGGCTACCTTGCTGTCCTTGACATTTATCAAAACAACTTTTTCATTGCACTATGCATGGAAGACAATGGCTATGGTACTGTCAATTGTATCTCTCTTCCCTTTATGCCTGTCCACGACCTCTCAAAAAACAACATGGCTTCCGGTGCTGTTGGGATCTCTTGGATGCAAACCACTAACCATGTTTCTTATAACAGAAAACAAAATCTGGGGAAGGAAAAGTTGGCCCCTCAATGAAGGAATTATGGCTGTTGGAATAGTTAGCATTCTACTAAGTTCACTCCTCAAAAATGATGTGCCGCTAGCCGGCCCACTAATAGCTGGAGGCATGCTAATAGCATGTTATGTCATATCCGGAAGCTCAGCCGATTTATCACTGGAGAAAGCGGCTGAGGTCTCCTGGGAAGAAGAAGCAGAACACTCTGGTGCCTCACACAACATACTAGTGGAGGTCCAAGATGATGGAACCATGAAGATAAAAGATGAAGAGAGAGATGACACACTCACCATTCTCCTTAAAGCAACTCTGCTGGCAGTCTCAGGGGTGTACCCAATGTCAATACCAGCGACCCTTTTTGTGTGGTATTTTTGGCAGAAAAAGAAACAGAGATCAGGAGTGCTATGGGACACACCCAGCCCTCCAGAAGTGGAAAGAGCAGTTCTTGATGATGGTATCTATAGAATTTTGCAAAGAGGACTGTTGGGCAGGTCCCAAGTAGGAGTAGGAGTTTTCCAAGAAGGCGTGTTCCACACAATGTGGCACGTCACCAGGGGAGCTGTCCTCATGTATCAAGGGAAGAGGCTGGAACCAAGCTGGGCCAGTGTCAAAAAAGACTTGATCTCATATGGAGGAGGTTGGAGGTTTCAAGGATCCTGGAACACGGGAGAAGAAGTGCAGGTGATTGCTGTTGAACCGGGAAAAAACCCCAAAAATGTACAGACAGCGCCGGGCACCTTCAAGACCCCTGAAGGCGAAGTTGGAGCCATAGCCCTAGATTTTAAACCCGGCACATCTGGATCTCCCATCGTGAACAGAGAGGGAAAAATAGTAGGTCTTTATGGAAATGGAGTGGTGACAACAAGTGGAACCTACGTCAGTGCCATAGCTCAAGCTAAAGCATCACAAGAAGGGCCTCTACCAGAGATTGAGGACGAGGTGTTTAGGAAAAGAAACTTAACAATAATGGACCTACATCCAGGATCGGGAAAAACAAGAAGATATCTTCCAGCCATAGTCCGTGAGGCCATAAAAAGGAAGCTGCGCACGCTAATCTTGGCTCCCACAAGAGTTGTCGCTTCTGAAATGGCAGAGGCGCTCAAGGGAATGCCAATAAGGTATCAGACAACAGCAGTGAAGAGTGAACACACAGGAAAGGAGATAGTTGACCTTATGTGCCACGCCACTTTCACCATGCGTCTCCTGTCTCCCGTGAGAGTTCCCAATTACAACATGATTATCATGGATGAAGCACATTTCACCGATCCAGCCAGCATAGCGGCCAGAGGGTACATCTCAACCCGAGTGGGTATGGGTGAAGCAGCTGCGATCTTTATGACAGCCACTCCCCCAGGATCGGTGGAGGCCTTTCCACAGAGCAATGCAGTTATCCAAGATGAGGAAAGAGACATTCCTGAGAGATCATGGAACTCAGGCTATGACTGGATCACTGATTTCCCAGGTAAAACAGTCTGGTTTGTTCCAAGCATCAAATCAGGAAATGACATTGCCAACTGTTTAAGAAAGAACGGGAAACGGGTGATCCAATTGAGCAGAAAAACCTTTGACACTGAGTACCAGAAAACAAAAAACAACGACTGGGACTATGTCGTCACAACAGACATTTCCGAAATGGGAGCAAACTTCCGGGCCGACAGGGTAATAGACCCAAGGCGGTGTCTGAAACCGGTAATACTAAAAGATGGTCCAGAGCGCGTCATTCTAGCCGGACCGATGCCAGTGACTGTGGCCAGTGCCGCCCAGAGGAGAGGAAGAATTGGAAGGAACCAAAACAAGGAAGGTGATCAGTATATTTACATGGGACAGCCTTTAAACAATGATGAGGACCACGCTCATTGGACAGAAGCAAAAATGCTCCTTGACAATATAAACACACCAGAAGGGATTATCCCAGCCCTCTTTGAGCCGGAGAGAGAAAAGAGTGCAGCAATAGACGGGGAATACAGACTGCGGGGTGAAGCAAGGAAAACGTTCGTGGAGCTCATGAGAAGAGGAGATCTACCTGTCTGGCTATCCTACAAAGTTGCCTCAGAAGGCTTCCAGTACTCCGACAGAAGGTGGTGCTTTGATGGGGAAAGGAACAACCAGGTGTTGGAGGAGAACATGGACGTGGAGATCTGGACAAAAGAAGGAGAAAGAAAGAAACTACGACCCCGCTGGTTGGATGCCAGAACATACTCTGACCCACTGGCTCTGCGCGAGTTTAAAGAGTTTGCAGCAGGAAGAAGAAGCGTCTCAGGTGACCTAATATTAGAAATAGGGAAACTTCCACAACATTTGACGCAAAGGGCCCAGAATGCTTTGGACAACTTGGTCATGTTGCACAATTCCGAACAAGGAGGAAAAGCCTATAGACATGCTATGGAAGAACTACCAGACACCATAGAAACGTTGATGCTCCTAGCTTTGATAGCTGTGTTGACTGGTGGAGTGACGCTGTTCTTCCTATCAGGAAGAGGTCTAGGGAAAACATCCATCGGCCTACTCTGCGTGATGGCCTCAAGCGTACTGTTATGGATGGCCAGTGTGGAGCCCCATTGGATAGCGGCCTCCATCATACTGGAGTTCTTTCTGATGGTGCTGCTTATTCCAGAGCCAGACAGACAGCGCACTCCACAGGACAACCAGCTAGCATATGTGGTGATAGGTTTGTTATTCATGATATTGACAGTGGCAGCCAATGAGATGGGATTATTGGAAACCACAAAGAAAGACCTGGGGATTGGCCATGTAGCTGCTGAAAACCACCACCATGCTACAATGCTGGACGTAGACCTACATCCAGCTTCAGCCTGGACCCTCTATGCAGTGGCCACAACAATTATCACTCCCATGATGAGACACACAATTGAAAACACAACGGCAAATATTTCCCTGACAGCTATTGCAAACCAGGCAGCTATATTGATGGGACTTGACAAGGGATGGCCAATATCGAAGATGGACATAGGAGTTCCACTTCTCGCCTTGGGGTGCTATTCCCAGGTGAACCCGCTGACGCTGACAGCGGCGGTATTGATGCTAGTGGCTCATTACGCCATAATTGGACCTGGACTGCAAGCAAAAGCTACTAGAGAAGCTCAAAAAAGGACAGCGGCCGGAATAATGAAAAATCCAACTGTCGACGGGATTGTTGCAATAGATTTGGATCCTGTGGTTTACGATGCAAAATTTGAAAAACAGCTAGGCCAAATAATGTTGTTGATACTTTGCACATCACAGATTCTCTTGATGCGGACTACATGGGCCTTGTGTGAATCCATCACACTGGCTACTGGACCTCTGACCACTCTTTGGGAGGGATCTCCAGGAAAATTCTGGAACACCACGATAGCGGTGTCCATGGCAAACATTTTCAGGGGAAGTTATCTAGCAGGAGCAGGTCTGGCCTTCTCATTAATGAAATCTCTAGGAGGAGGTAGGAGAGGCACGGGAGCCCAAGGGGAAACACTGGGAGAAAAATGGAAAAGACAGCTAAACCAACTGAGCAAGTCAGAATTCAACACTTACAAAAGGAGTGGGATTATGGAGGTGGATAGATCCGAAGCCAAAGAGGGACTGAAAAGAGGAGAAACAACCAAACATGCAGTGTCGAGAGGAACAGCCAAACTGAGGTGGTTTGTGGAGAGGAACCTTGTGAAACCAGAAGGGAAAGTCATAGACCTCGGTTGTGGAAGAGGTGGCTGGTCATATTATTGCGCTGGGCTGAAGAAAGTCACAGAAGTGAAAGGATACACAAAAGGAGGACCTGGACATGAGGAACCAATCCCAATGGCGACCTATGGATGGAACCTAGTAAAGCTACACTCCGGAAAAGATGTATTCTTTACACCACCTGAGAAATGTGACACCCTTTTGTGTGATATTGGTGAGTCCTCTCCGAACCCAACTATAGAAGAAGGAAGAACGTTACGTGTTCTAAAGATGGTGGAACCATGGCTCAGAGGAAACCAATTTTGCATAAAAATTCTAAATCCCTATATGCCGAGTGTGGTAGAAACTCTGGAGCAAATGCAAAGAAAACATGGAGGAATGCTAGTGCGAAACCCACTCTCAAGAAATTCCACCCATGAAATGTACTGGGTTTCATGTGGAACAGGAAACATTGTGTCAGCAGTAAACATGACATCTAGAATGTTGCTAAATCGATTCACAATGGCTCACAGGAAGCCAACATATGAAAGAGACGTGGACTTAGGCGCTGGAACAAGACATGTGGCAGTGGAACCAGAGGTAGCCAACCTAGATATCATTGGCCAGAGGATAGAGAATATAAAAAATGAACATAAGTCAACATGGCATTATGATGAGGACAATCCATACAAAACATGGGCCTATCATGGATCATATGAGGTCAAGCCATCAGGATCAGCCTCATCCATGGTCAATGGCGTGGTGAGATTGCTCACCAAACCATGGGATGTTATCCCCATGGTCACACAAATAGCCATGACTGACACCACACCCTTTGGACAACAGAGGGTGTTTAAAGAGAAAGTTGACACGCGCACACCAAAAGCAAAACGAGGCACAGCACAAATTATGGAGGTGACAGCCAGGTGGTTATGGGGTTTCCTTTCTAGAAACAAAAAACCCAGAATCTGCACAAGAGAGGAGTTCACAAGAAAAGTTAGGTCAAACGCAGCTATTGGAGCAGTGTTCGTTGATGAAAATCAATGGAACTCAGCAAAAGAAGCAGTGGAAGACGAACGGTTCTGGGACCTTGTGCACAGAGAGAGGGAGCTTCATAAACAGGGAAAATGTGCCACGTGTGTCTACAATATGATGGGGAAGAGAGAGAAAAAATTAGGAGAGTTCGGAAAGGCAAAAGGAAGTCGTGCAATATGGTACATGTGGTTGGGAGCACGCTTTCTAGAGTTCGAAGCCCTTGGTTTCATGAATGAAGATCACTGGTTCAGTAGAGAGAATTCACTCAGTGGAGTGGAAGGAGAAGGACTCCACAAACTTGGATACATACTCAGAGACATATCAAAGATTCCAGGGGGAAATATGTATGCAGATGACACAGCCGGATGGGACACAAGAATAACAGAGGATGATCTTCAGAATGAGGCTAAAATCACTGACATCATGGAGCCCGAACATGCCCTATTGGCTACGTCAATCTTTAAGCTGACCTACCAAAATAAGGTGGTAAGGGTGCAGAGACCAGCAAAAAATGGAACCGTGATGGATGTTATATCCAGACGTGACCAGAGAGGAAGTGGACAGGTCGGAACTTATGGCTTAAACACTTTCACCAACATGGAGGCCCAACTAATAAGACAAATGGAGTCTGAGGGAATCTTTTTACCCAGCGAATTGGAAACCCCAAATCTAGCCGAAAGAGTTCTCGACTGGTTGGAAAAACATGGCGTCGAAAGGCTGAAAAGAATGGCAATCAGCGGAGATGACTGTGTGGTGAAACCAATTGATGACAGGTTCGCAACAGCCTTAACAGCTTTGAATGACATGGGAAAAGTAAGAAAAGACATACCGCAATGGGAACCTTCAAAAGGATGGAATGATTGGCAACAAGTGCCTTTCTGTTCACACCATTTCCACCAGCTGATTATGAAGGATGGGAGGGAGATAGTGGTGCCATGCCGCAACCAAGATGAACTTGTGGGTAGGGCCAGAGTATCACAAGGCGCCGGATGGAGCCTGAGAGAAACTGCATGCCTAGGCAAGTCATATGCACAAATGTGGCAGCTGATGTACTTCCACAGGAGAGACCTGAGACTAGCGGCTAATGCTATCTGTTCAGCCGTTCCAGTTGATTGGGTCCCAACCAGCCGCACCACCTGGTCGATCCATGCCCATCACCAATGGATGACAACAGAAGACATGTTATCAGTGTGGAATAGGGTTTGGATAGAGGAAAACCCATGGATGGAGGATAAAACTCATGTATCCAGTTGGGAAGATGTTCCATACTTAGGAAAAAGGGAAGATCAGTGGTGTGGATCCCTGATAGGCTTAACAGCAAGGGCCACCTGGGCCACTAACATACAAGTGGCCATAAACCAAGTGAGAAGGCTCATTGGGAATGAGAATTATCTAGATTACATGACATCAATGAAGAGATTCAAGAATGAGAGTGATCCCGAAGGGGCACTCTGGTAAGTCAACACATTCACAAAATAAAGGAAAATAAAAAATCAAACAAGGCAAGAAGTCAGGCCGGATTAAGCCATAGTACGGTAAGAGCTATGCTGCCTGTGAGCCCCGTCCAAGGACGTAAAATGAAGTCAGGCCGAAAGCCACGGTTTGAGCAAACCGTGCTGCCTGTGGCTCCATCGTGGGGATGTAAAAACCCGGGAGGCTGCAACCCATGGAAGCTGTACGCATGGGGTAGCAGACTAGTGGTTAGAGGAGACCCCTCCCAAGACACAACGCAGCAGCGGGGCCCAACACCAGGGGAAGCTGTACCCTGGTGGTAAGGACTAGAGGTTAGAGGAGACCCCCCGCACAACAATAAACAGCATATTGACGCTGGGAGAGACCAGAGATCCTGCTGTCTCTACAGCATCATTCCAGGCACAGAACGCCAGAAAATGGAATGGTGCTGTTGAATCAACAGGTTCT