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charles-plessy committed Jul 25, 2024
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Expand Up @@ -61,7 +61,7 @@ Genomes are aligned witn [`lastal`](https://gitlab.com/mcfrith/last/-/blob/main/

Dot plots representing the pairwise genome alignments, produced with the [`last-dotplot`](https://gitlab.com/mcfrith/last/-/blob/main/doc/last-dotplot.rst) tool. In the one-to-one alignment example below, the `hg38` human genome (_target_) is represented on the horizontal axis and a monkey genopme (_Macaca mulatta_ accession number `GCA\_003339765.3`) on the vertical axis (_query_). Regions containing unknown (`N`) sequences are on pink background. Forward (+/+) alignments are plotted in red and reverse (+/– or –/+) in blue. _Target_ (human) contigs are displayed in their original order. _Query_ contigs (monkey) are reordered and possibly reverse-complemented to diagonalise the plot as much as possible. The names of reverse-complemented contigs are printed in blue.

![Example of a dot-plot produced by the pipeline after aligning human and macaque genomes](images/Homo_sapiens_GCA_000001405.29_GRCh38.p14___Macaca_mulatta_GCA_003339765.3.o2o_plt.png "Human–Monkey comparison")
![Example of a dot-plot produced by the pipeline after aligning human and macaque genomes](images/Homo_sapiens_GCA_000001405.29_GRCh38.p14___Macaca_mulatta_GCA_003339765.3.o2o_plt.png "Human–Monkey comparison")

### `N` regions

Expand Down Expand Up @@ -99,7 +99,7 @@ The example MultiQC plots below were generated on this pipeline's full test data

The pipeline reports the base content of every query genome, like in the example below:

![Example of a base content report for primate genomes](images/mqc_base_content_summary-pct.png "Primate genome base content")
![Example of a base content report for primate genomes](images/mqc_base_content_summary-pct.png "Primate genome base content")

#### Contig length statistics

Expand All @@ -111,13 +111,13 @@ Contig length statistics can be displayed by MultiQC as violin plots.

Alignment parameters computed by `last-train` can be displayed by MultiQC as violin plots.

![Example of alignment parameters for primate genomes aligned to the human genome](images/mqc_train-stats.png "Alignment parameters")
![Example of alignment parameters for primate genomes aligned to the human genome](images/mqc_train-stats.png "Alignment parameters")

#### Alignment

Alignment statistics can be displayed by MultiQC as violin plots.

![Example of alignment statistics for primate genomes aligned to the human genome](images/mqc_last_o2o-stats.png "Alignment statistics")
![Example of alignment statistics for primate genomes aligned to the human genome](images/mqc_last_o2o-stats.png "Alignment statistics")

### Pipeline information

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