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#!/usr/bin/env python3 | ||
import urllib.request | ||
import zlib | ||
from ftplib import FTP | ||
import sys | ||
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def main(): | ||
# SRR12478073 is a good test | ||
accession,skip,sample = sys.argv[1:] | ||
url = get_url(accession) | ||
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sample_url(url,int(skip),int(sample)) | ||
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def sample_url(url,skip,sample): | ||
url_sections = url.split("/") | ||
server = url_sections[0] | ||
file = url_sections[-1] | ||
folder = "/".join(url_sections[1:-1]) | ||
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decompressor = collect_gzip_data(skip,sample) | ||
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#print(f"URL is {url} server={server} folder={folder} file={file}") | ||
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#print(f"Connecting to {server}") | ||
ftp = FTP(server) | ||
#print(f"Logging in") | ||
ftp.login() | ||
#print(f"Moving to {folder}") | ||
ftp.cwd(folder) | ||
ftp.retrbinary("RETR "+file,decompressor, blocksize=124) | ||
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ftp.quit() | ||
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def collect_gzip_data(skip,collect): | ||
decompressor = zlib.decompressobj(zlib.MAX_WBITS|16) | ||
newline_count = 0 | ||
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def accept_data(chunk): | ||
nonlocal decompressor | ||
nonlocal newline_count | ||
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new_data = decompressor.decompress(chunk).decode('utf-8') | ||
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lines = new_data.split("\n") | ||
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for i in range(len(lines)): | ||
if newline_count >= 4*skip: | ||
print(lines[i],end='') | ||
if i < len(lines)-1: | ||
print("\n",end='') | ||
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if i < len(lines)-1: | ||
newline_count += 1 | ||
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if newline_count >= 4*(skip+collect): | ||
sys.exit() | ||
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return(accept_data) | ||
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def get_url(accession): | ||
# Get the FTP locations for the sample | ||
ena_rest_url = f"https://www.ebi.ac.uk/ena/portal/api/filereport?accession={accession}&result=read_run&fields=run_accession,fastq_ftp" | ||
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with urllib.request.urlopen(ena_rest_url) as response: | ||
if response.getcode() != 200: | ||
raise IOError(f"[ENA] Got error code {response.getcode()} from ENA REST for accession {sample['accession']}") | ||
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url = "" | ||
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found_accession_line = False | ||
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for line in response: | ||
line = line.decode("UTF-8").strip() | ||
#print(line) | ||
if line.startswith("SRR"): | ||
found_accession_line = True | ||
sections = line.split("\t") | ||
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if len(sections) != 2: | ||
raise IOError(f"[ENA] No ENA fastq files found for accession {sample['accession']}") | ||
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url = sections[1].split(";")[0] # Just take the first read if there are multiple available | ||
return(url) | ||
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raise IOError(f"[ENA] Found no accession in response from ENA REST for accession {sample['accession']}") | ||
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if __name__ == "__main__": | ||
main() |