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Hi! I am having a problem annotating a VCF file of structural variants. I just want to add tags to the INFO field by ID but I get this error:
bcftools annotate -a ${ANNOT_FILE}.gz -h ${HDR_FILE} -c CHROM,POS,~ID,REF,ALT,INFO/n_hits,INFO/fragmts,INFO/match_lengths,INFO/repeat_ids,INFO/matching_classes,INFO/RM_hit_strands,INFO/RM_hit_IDs,INFO/total_match_length,INFO/total_match_span ${ANNOT_FILE}.genotypes.sorted.vcf
The tag "~ID" is not defined in ${ANNOT_FILE}.gz
${ANNOT_FILE}.gz looks like this
chr1 641270 oenPle.INS.73S0 A TTTGTGATATAACTAAAGCCAATTCCAATGCCCCATTTTCCTCATAAAAATTAAAAACAAGC 1 20 SINE + 2 3 20 20
and it was created as follows together with the header:
bgzip ${ANNOT_FILE}
tabix -s1 -b2 -e2 ${ANNOT_FILE}.gz
HDR_FILE=${ANNOT_FILE}.header
echo -e '##INFO=<ID=n_hits,Number=1,Type=Integer,Description="Number of repeats found in insertion">' >> ${HDR_FILE}
echo -e '##INFO=<ID=match_lengths,Number=.,Type=Integer,Description="Insertion lengths spanned by each repeat">' >> ${HDR_FILE}
echo -e '##INFO=<ID=repeat_ids,Number=.,Type=String,Description="Repeat family IDs">' >> ${HDR_FILE}
echo -e '##INFO=<ID=matching_classes,Number=.,Type=String,Description="Repeat class names">' >> ${HDR_FILE}
echo -e '##INFO=<ID=fragmts,Number=.,Type=Integer,Description="Number of fragments merged into one by one code">' >> ${HDR_FILE}
echo -e '##INFO=<ID=RM_hit_strands,Number=.,Type=String,Description="RepeatMasker hit strands">' >> ${HDR_FILE}
echo -e '##INFO=<ID=RM_hit_IDs,Number=.,Type=String,Description="RepeatMasker hit IDs">' >> ${HDR_FILE}
echo -e '##INFO=<ID=total_match_length,Number=1,Type=Integer,Description="Insertion length spanned by repeats">' >> ${HDR_FILE}
echo -e '##INFO=<ID=total_match_span,Number=1,Type=Float,Description="Insertion span spanned by repeats">' >> ${HDR_FILE}
echo -e '##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">' >> ${HDR_FILE}
How can I solve the error?
The text was updated successfully, but these errors were encountered:
Hi! I am having a problem annotating a VCF file of structural variants. I just want to add tags to the INFO field by ID but I get this error:
${ANNOT_FILE}.gz
looks like thisand it was created as follows together with the header:
How can I solve the error?
The text was updated successfully, but these errors were encountered: