compress counts

Makefile.am

@@ -217,6 +217,8 @@ dnmtools_SOURCES += src/utils/fast-liftover.cpp
 dnmtools_SOURCES += src/utils/covered.cpp
 dnmtools_SOURCES += src/utils/recovered.cpp
 dnmtools_SOURCES += src/utils/kmersites.cpp
+dnmtools_SOURCES += src/utils/xcounts.cpp
+dnmtools_SOURCES += src/utils/unxcounts.cpp
 
 dnmtools_SOURCES += src/amrfinder/allelicmeth.cpp
 dnmtools_SOURCES += src/amrfinder/amrfinder.cpp

src/analysis/methcounts.cpp

@@ -48,6 +48,8 @@ using std::unordered_set;
 using std::unordered_map;
 
 using bamxx::bam_rec;
+using bamxx::bam_header;
+using bamxx::bgzf_file;
 
 struct quick_buf : public std::ostringstream,
                    public std::basic_stringbuf<char> {
@@ -218,7 +220,7 @@ tag_with_mut(const uint32_t tag, const bool mut) {
 
 template <const bool require_covered = false>
 static void
-write_output(const bamxx::bam_header &hdr, bamxx::bgzf_file &out,
+write_output(const bam_header &hdr, bgzf_file &out,
              const int32_t tid, const string &chrom,
              const vector<CountSet> &counts, bool CPG_ONLY) {
 
@@ -322,7 +324,7 @@ count_states_neg(const bam_rec &aln, vector<CountSet> &counts) {
 
 
 static unordered_map<int32_t, size_t>
-get_tid_to_idx(const bamxx::bam_header &hdr,
+get_tid_to_idx(const bam_header &hdr,
                const unordered_map<string, size_t> name_to_idx) {
   unordered_map<int32_t, size_t> tid_to_idx;
   for (int32_t i = 0; i < hdr.h->n_targets; ++i) {
@@ -342,15 +344,15 @@ template <class CS>
 static void
 output_skipped_chromosome(const bool CPG_ONLY, const int32_t tid,
                           const unordered_map<int32_t, size_t> &tid_to_idx,
-                          const bamxx::bam_header &hdr,
+                          const bam_header &hdr,
                           const vector<string>::const_iterator chroms_beg,
                           const vector<size_t> &chrom_sizes,
-                          vector<CS> &counts, bamxx::bgzf_file &out) {
+                          vector<CS> &counts, bgzf_file &out) {
 
   // get the index of the next chrom sequence
   const auto chrom_idx = tid_to_idx.find(tid);
   if (chrom_idx == cend(tid_to_idx))
-    throw dnmt_error("chrom not found: " + string(sam_hdr_tid2name(hdr, tid)));
+    throw dnmt_error("chrom not found: " + sam_hdr_tid2name(hdr, tid));
 
   const auto chrom_itr = chroms_beg + chrom_idx->second;
 
@@ -363,15 +365,15 @@ output_skipped_chromosome(const bool CPG_ONLY, const int32_t tid,
 
 
 static bool
-consistent_targets(const bamxx::bam_header &hdr,
+consistent_targets(const bam_header &hdr,
                    const unordered_map<int32_t, size_t> &tid_to_idx,
                    const vector<string> &names, const vector<size_t> &sizes) {
   const size_t n_targets = hdr.h->n_targets;
   if (n_targets != names.size()) return false;
 
   for (size_t tid = 0; tid < n_targets; ++tid) {
-    const string tid_name_sam = sam_hdr_tid2name(hdr.h, tid);
-    const size_t tid_size_sam = sam_hdr_tid2len(hdr.h, tid);
+    const string tid_name_sam = sam_hdr_tid2name(hdr, tid);
+    const size_t tid_size_sam = sam_hdr_tid2len(hdr, tid);
     const auto idx_itr = tid_to_idx.find(tid);
     if (idx_itr == cend(tid_to_idx)) return false;
     const auto idx = idx_itr->second;
@@ -380,13 +382,24 @@ consistent_targets(const bamxx::bam_header &hdr,
   return true;
 }
 
-
-template<const bool require_covered = false>
 static void
+write_header(const bam_header &hdr, bgzf_file &out) {
+  for (auto i = 0; i < hdr.h->n_targets; ++i) {
+    const size_t tid_size = sam_hdr_tid2len(hdr, i);
+    const string tid_name = sam_hdr_tid2name(hdr, i);
+    std::ostringstream oss;
+    oss << "# " << tid_name << ' ' << tid_size << '\n';
+    out.write(oss.str());
+  }
+  out.write("# end_header\n");
+}
+
+template<const bool require_covered = false> static void
 process_reads(const bool VERBOSE, const bool show_progress,
-              const bool compress_output, const size_t n_threads,
-              const string &infile, const string &outfile,
-              const string &chroms_file, const bool CPG_ONLY) {
+              const bool compress_output, const bool include_header,
+              const size_t n_threads, const string &infile,
+              const string &outfile, const string &chroms_file,
+              const bool CPG_ONLY) {
   // first get the chromosome names and sequences from the FASTA file
   vector<string> chroms, names;
   read_fasta_file_short_names(chroms_file, names, chroms);
@@ -410,7 +423,7 @@ process_reads(const bool VERBOSE, const bool show_progress,
   bamxx::bam_in hts(infile);
   if (!hts) throw dnmt_error("failed to open input file");
   // load the input file's header
-  bamxx::bam_header hdr(hts);
+  bam_header hdr(hts);
   if (!hdr) throw dnmt_error("failed to read header");
 
   unordered_map<int32_t, size_t> tid_to_idx = get_tid_to_idx(hdr, name_to_idx);
@@ -420,7 +433,7 @@ process_reads(const bool VERBOSE, const bool show_progress,
 
   // open the output file
   const string output_mode = compress_output ? "w" : "wu";
-  bamxx::bgzf_file out(outfile, output_mode);
+  bgzf_file out(outfile, output_mode);
   if (!out) throw dnmt_error("error opening output file: " + outfile);
 
   // set the threads for the input file decompression
@@ -429,6 +442,9 @@ process_reads(const bool VERBOSE, const bool show_progress,
     tp.set_io(out);
   }
 
+  if (include_header)
+    write_header(hdr, out);
+
   // now iterate over the reads, switching chromosomes and writing
   // output as needed
   bam_rec aln;
@@ -499,6 +515,7 @@ main_counts(int argc, const char **argv) {
     bool CPG_ONLY = false;
     bool require_covered = false;
     bool compress_output = false;
+    bool include_header = false;
 
     string chroms_file;
     string outfile;
@@ -519,6 +536,8 @@ main_counts(int argc, const char **argv) {
                       false, CPG_ONLY);
     opt_parse.add_opt("require-covered", 'r', "only output covered sites",
                       false, require_covered);
+    opt_parse.add_opt("header", 'H', "add a header to identify the reference",
+                      false, include_header);
     opt_parse.add_opt("zip", 'z', "output gzip format", false, compress_output);
     opt_parse.add_opt("progress", '\0', "show progress", false, show_progress);
     opt_parse.add_opt("verbose", 'v', "print more run info", false, VERBOSE);
@@ -559,12 +578,12 @@ main_counts(int argc, const char **argv) {
 
     if (require_covered)
       process_reads<true>(VERBOSE, show_progress, compress_output,
-                          n_threads, mapped_reads_file, outfile,
+                          include_header, n_threads, mapped_reads_file, outfile,
                           chroms_file, CPG_ONLY);
     else
-      process_reads(VERBOSE, show_progress, compress_output,
-                    n_threads, mapped_reads_file, outfile,
-                    chroms_file, CPG_ONLY);
+      process_reads(VERBOSE, show_progress, compress_output, include_header,
+                    n_threads, mapped_reads_file, outfile, chroms_file,
+                    CPG_ONLY);
   }
   catch (const std::exception &e) {
     cerr << e.what() << endl;

src/common/MSite.cpp

@@ -34,15 +34,14 @@ using std::cbegin;
 using std::cend;
 using std::end;
 
-MSite::MSite(const string &line) {
+
+bool
+MSite::initialize(const char *c, const char *c_end) {
   constexpr auto is_sep = [](const char x) { return x == ' ' || x == '\t'; };
   constexpr auto not_sep = [](const char x) { return x != ' ' && x != '\t'; };
 
   bool failed = false;
 
-  const auto c = line.data();
-  const auto c_end = c + line.size();
-
   auto field_s = c;
   auto field_e = find_if(field_s + 1, c_end, is_sep);
   if (field_e == c_end) failed = true;
@@ -97,14 +96,25 @@ MSite::MSite(const string &line) {
     const auto [ptr, ec] = from_chars(field_s, c_end, n_reads);
     failed = failed || (ptr != c_end);
   }
+  // ADS: the value below would work for a flag
+  // pos = std::numeric_limits<decltype(pos)>::max();
+
+  return !failed;
+}
+
 
-  if (failed) {
+MSite::MSite(const string &line) {
+  if (!initialize(line.data(), line.data() + size(line)))
     throw runtime_error("bad count line: " + line);
-    // ADS: the value below would work for a flag
-    // pos = std::numeric_limits<decltype(pos)>::max();
-  }
 }
 
+
+MSite::MSite(const char *line, const int n) {
+  if (!initialize(line, line + n))
+    throw runtime_error("bad count line: " + string(line));
+}
+
+
 string
 MSite::tostring() const {
   std::ostringstream oss;

src/common/MSite.hpp

@@ -35,6 +35,9 @@ struct MSite {
     chrom(_chrom), pos(_pos), strand(_strand),
     context(_context), meth(_meth), n_reads(_n_reads) {}
   explicit MSite(const std::string &line);
+  explicit MSite(const char *line, const int n);
+
+  bool initialize(const char *c, const char *c_end);
 
   std::string chrom;
   size_t pos;

src/common/bam_record_utils.hpp

@@ -263,6 +263,11 @@ sam_hdr_tid2name(const bamxx::bam_header &hdr, const int32_t tid) {
   return std::string(sam_hdr_tid2name(hdr.h, tid));
 }
 
+inline uint32_t
+sam_hdr_tid2len(const bamxx::bam_header &hdr, const int32_t tid) {
+  return sam_hdr_tid2len(hdr.h, tid);
+}
+
 inline std::string
 sam_hdr_tid2name(const bamxx::bam_header &hdr, const bamxx::bam_rec &aln) {
   return std::string(sam_hdr_tid2name(hdr.h, aln.b->core.tid));

src/dnmtools.cpp

@@ -128,6 +128,10 @@ metagene(int argc, const char **argv);
 int
 main_covered(int argc, const char **argv);
 int
+main_xcounts(int argc, const char **argv);
+int
+main_unxcounts(int argc, const char **argv);
+int
 main_recovered(int argc, const char **argv);
 int
 kmersites(int argc, const char **argv);
@@ -200,6 +204,8 @@ main(int argc, const char **argv) {
    {"merge",         "merge multiple counts files into a counts file or a table", main_merge_methcounts},
    {"covered",       "filter a counts file for only covered sites", main_covered},
    {"recovered",     "replace missing sites in a counts file", main_recovered},
+   {"xcounts",       "compress counts files by removing information", main_xcounts},
+   {"unxcounts",     "reverse the xcounts process yielding a counts file", main_unxcounts},
    {"selectsites",   "sites inside a set of genomic intervals", main_selectsites},
    {"kmersites",     "make track file for sites matching kmer", kmersites}}}}}};
 // clang-format on

src/utils/covered.cpp

@@ -81,7 +81,7 @@ main_covered(int argc, const char **argv) {
 
     /****************** COMMAND LINE OPTIONS ********************/
     OptionParser opt_parse(strip_path(argv[0]), description,
-                           "<methcounts-file> (\"-\" for standard input)", 1);
+                           "<counts-file> (\"-\" for standard input)", 1);
     opt_parse.add_opt("output", 'o', "output file (default is standard out)",
                       false, outfile);
     opt_parse.add_opt("threads", 't', "threads for compression (use few)",

src/utils/recovered.cpp

@@ -67,6 +67,7 @@ verify_chrom_orders(const bool verbose, const uint32_t n_threads,
   int32_t prev_idx = -1;
 
   while (getline(in, line)) {
+    if (line[0] == '#') continue;
     line.resize(line.find_first_of(" \t"));
     if (line != prev_chrom) {
       if (verbose) cerr << "verifying: " << line << endl;
@@ -273,8 +274,11 @@ process_sites(const bool verbose, const bool add_missing_chroms,
   // ADS: this is probably a poor strategy since we already would know
   // the index of the chrom sequence in the vector.
   chrom_itr_t chrom_itr;
+  string line;
 
-  while (read_site(in, site)) {
+  while (getline(in, line)) {
+    if (line[0] == '#') continue;
+    site.initialize(line.data(), line.data() + size(line));
     if (site.chrom != chrom_name) {
 
       if (pos != num_lim<uint64_t>::max())