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Merge pull request #52 from knollr/patch-1
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add conda channels for samtools, scanpy, muon
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wxicu authored May 18, 2024
2 parents 6441e66 + cae0b60 commit a90bb7c
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Showing 3 changed files with 4 additions and 4 deletions.
2 changes: 1 addition & 1 deletion modules/multi/gene_demulti/demuxlet.nf
Original file line number Diff line number Diff line change
Expand Up @@ -5,7 +5,7 @@ nextflow.enable.dsl=2

process subset_bam_and_sort_vcf_based_on_reference{
label 'small_mem'
conda "bioconda::samtools=1.19.2 bedtools bcftools=1.19"
conda "-c conda-forge -c bioconda samtools=1.19.2 bedtools bcftools=1.19"
tag "${sampleId}"
input:
tuple val(sampleId), path(sam), path(sam_index), path(barcodes), val(vcf)
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2 changes: 1 addition & 1 deletion modules/multi/gene_demulti/freemuxlet.nf
Original file line number Diff line number Diff line change
Expand Up @@ -4,7 +4,7 @@ nextflow.enable.dsl=2

process subset_bam_and_sort_vcf_based_on_reference{
label 'small_mem'
conda "bioconda::samtools=1.19.2 bedtools bcftools=1.19"
conda "-c conda-forge -c bioconda samtools=1.19.2 bedtools bcftools=1.19"
tag "${sampleId}"
input:
tuple val(sampleId), path(sam), path(sam_index), path(group_list), val(nsample)
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4 changes: 2 additions & 2 deletions modules/multi/gene_demultiplexing.nf
Original file line number Diff line number Diff line change
Expand Up @@ -24,7 +24,7 @@ def split_input(input){
process subset_bam_to_comon_variants{

label 'small_mem'
conda "bioconda::samtools=1.19.2 bedtools bcftools=1.19"
conda "-c conda-forge -c bioconda samtools=1.19.2 bedtools bcftools=1.19"
tag "${sampleId}"
input:
tuple val(sampleId), path(sam), path(sam_index), path(barcodes)
Expand All @@ -45,7 +45,7 @@ process summary{
publishDir "$params.outdir/$sampleId/$params.mode/gene_demulti", mode: 'copy'
label 'small_mem'
tag "${sampleId}"
conda "pandas scanpy mudata"
conda "-c conda-forge pandas scanpy mudata"

input:
tuple(val(sampleId), path(hto_matrix, stageAs: 'hto_data'), path(rna_matrix, stageAs: 'rna_data'), val(souporcell_result), val(scsplit_result), val(vireo_result),val(freemuxlet_result),val(demuxlet_result))
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