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Reference sequences and job scripts for RNA editing and FLAM-seq analysis of PacBio CCS reads, used in "The RNA-binding protein Musashi controls axon compartment-specific synaptic connectivity through ptp69D mRNA poly(A)-tailing"

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weihongqi/p3154

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DOI

The RNA-binding protein Musashi controls axon compartment-specific synaptic connectivity through ptp69D mRNA poly(A)-tailing

María Landínez-Macías, Weihong Qi, Anna Bratus-Neuenschwander, Martin Müller, and Olivier Urwyler

Reference sequences

*.fasta

RNA editing analysis of PacBio CCS reads

input.txt: input configuration for minimap2_lofreq.sh

minimap2_lofreq.sh: align reads and variant calling

splitSNP.*.sh: split reads by variants in various input bam files

venn.R: venn diagram analysis of alleleic reads

FLAM-seq analysis pf PacBio CCS reads

cutadapt-blat.sh: adapter trimming, polyA tail identification using cutadpat and blat

get_map_tail.pl: combined analysis of polyA tails identified by cutadapt and blat

FLAM-seq.Rmd: clustering of reads into isoforms by polyA tails

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Reference sequences and job scripts for RNA editing and FLAM-seq analysis of PacBio CCS reads, used in "The RNA-binding protein Musashi controls axon compartment-specific synaptic connectivity through ptp69D mRNA poly(A)-tailing"

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