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code spell
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psobolewskiPhD committed Feb 23, 2024
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14 changes: 13 additions & 1 deletion .vscode/settings.json
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@@ -1,5 +1,17 @@
{
"markdown.copyFiles.destination": {
"*": "images/"
}
},
"cSpell.enableFiletypes": [
"quarto"
],
"cSpell.ignoreWords": [
"callout",
"transparentback"
],
"cSpell.words": [
"autoset",
"Bankhead",
"Prefilter"
]
}
17 changes: 9 additions & 8 deletions index.qmd
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Expand Up @@ -63,7 +63,7 @@ QuPath has a graphical user interface (`GUI`) for performant working with very l

- Was designed for very large, multiscale 2D files

- Includes extensitve annotation, overlay, and visualization options
- Includes extensive annotation, overlay, and visualization options

- It includes robust algorithms for common analysis tasks

Expand Down Expand Up @@ -236,7 +236,7 @@ QuPath can handle large 2D images, e.g. whole-slide images—*there is no need t

## QuPath toolbar: information

![](images/QuPath_info_tools.png){fig-alt="Screenshot of QuPath application window with the Prefereces, Log, and Interactive Help tools marked in red." fig-align="center"}
![](images/QuPath_info_tools.png){fig-alt="Screenshot of QuPath application window with the Preferences, Log, and Interactive Help tools marked in red." fig-align="center"}

## QuPath toolbar: information

Expand Down Expand Up @@ -273,7 +273,7 @@ QuPath can handle large 2D images, e.g. whole-slide images—*there is no need t
- Slider controls opacity, so you can see through to the data beneath

:::{.callout-important}
### If you can't see things you expect, check that they arn't hidden!
### If you can't see things you expect, check that they aren't hidden!
:::

## QuPath toolbar: measurements table & scripting
Expand All @@ -298,7 +298,7 @@ QuPath can handle large 2D images, e.g. whole-slide images—*there is no need t
## Annotation tool tips and tricks

- Annotations are editable, unless you lock them (Right-click ‣ Lock)
- Move tool can be toggled with `Spacebar`
- Move tool can be toggled with `Space bar`
- Double-click to *select* objects
- For Ellipse & Rectangle can also use Objects ‣ Annotations… ‣ Specify annotation
- To start fresh: Objects ‣ Delete… ‣ Delete all annotations
Expand All @@ -310,7 +310,7 @@ QuPath can handle large 2D images, e.g. whole-slide images—*there is no need t
- start from the *inside* of the annotation, to expand it
- Note: if you want to make an Annotation *inside* another Annotation, you need to first lock it.
- hold `Alt/Option` and start from the *outside* to reduce it
- Use `Shift` with the Brush or Wand to add to an Annotation (discontinous)
- Use `Shift` with the Brush or Wand to add to an Annotation (discontinuous)

## Analysis Pane: Annotation tab

Expand Down Expand Up @@ -415,8 +415,8 @@ QuPath can handle large 2D images, e.g. whole-slide images—*there is no need t
## Visual stain editor

- At the bottom are settings for `Auto` vector estimation
- Click `Auto` to get an intial estimate
- Look at the color panels, which are views at the 3D colorspace from orthogonal sides
- Click `Auto` to get an initial estimate
- Look at the color panels, which are views at the 3D color space from orthogonal sides
- if you have other pixels, can try to exclude them (checkbox) and run Auto again
- want the vectors to encompass the reddish-pink-purple pixels (those of H&E)
- Can manually tweak the vectors by using the handles
Expand Down Expand Up @@ -492,6 +492,7 @@ We are segmenting 4 large tissue slices, so we want to make:

We saved our classifier (thresholder), so we can re-use it:

![](images/load_pixel_classifier_menu.png){fig-alt="Screenshot of QuPath `Classify` menu showinf the `Load classifier` dialog." fig-align="center"}
![](images/load_pixel_classifier_menu.png){fig-alt="Screenshot of QuPath `Classify` menu showing the `Load classifier` dialog." fig-align="center"}

![](images/load_pixel_classifier.png){fig-alt="Screenshot of QuPath `Load pixel classifier` dialog." fig-align="center"}

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