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Add lesson plan
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psobolewskiPhD committed Feb 27, 2024
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## Welcome

## Lesson Plan

- Brief introduction to QuPath
- Key concept: Projects
- QuPath GUI layout and toolbars
- Making & Managing Annotations
- Setting stain Vectors
- Pixel classification using a thresholder

## What is QuPath?

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- YouTube Channel: [https://www.youtube.com/c/QuPath](https://www.youtube.com/c/QuPath)
- Image.sc Forums: [https://forum.image.sc/tag/qupath](https://forum.image.sc/tag/qupath)

## Key concept: QuPath `project`
# Key concept: QuPath `project`

- `projects` are the best way to organize your analysis work
- `projects` are frequently required by some scripts/extensions
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- Measurement Table drop-down menu enables opening a table view of measurements associated with different object types
- The Script Editor is used for automation and advanced tasks

## QuPath GUI Pro-tip: Command list
# QuPath GUI Pro-tip: Command list

:::{.callout-tip}
### For menu items, press `Command/Control-L` and start typing what you remember to get the menu item/command!
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# Setting stain vectors

## Start with a clean copy

- Let's start by duplicating our image:
- Analysis Pane ‣ Projects tab
- Right-click the image and select `Duplicate`
- **Un-check** `Also duplicate data files`

![](images/duplicate_image_no_data.png){fig-alt="Screenshot of QuPath `Duplicate image` dialog, with `Also duplicate data files` in red." fig-align="center"}

## Stain separation/deconvolution

- Aims to extract information about individual chromogenic stains from the RGB values of each pixel
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- When you select `image type` on import, default separation is performed

## Start with a clean copy

- Let's start by duplicating our image:
- Analysis Pane ‣ Projects tab
- Right-click the image and select `Duplicate`
- **Un-check** `Also duplicate data files`

![](images/duplicate_image_no_data.png){fig-alt="Screenshot of QuPath `Duplicate image` dialog, with `Also duplicate data files` in red." fig-align="center"}

## Setting stain vectors

- Use Annotation tools to make a small Annotation that includes:
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