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WIP: Busco ideograms #82

Merged
merged 8 commits into from
Nov 13, 2024
Merged

WIP: Busco ideograms #82

merged 8 commits into from
Nov 13, 2024

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chriswyatt1
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Added a function to use the Ideograms R plot function to add the genome+annotation busco scores per gene onto chromosome scaffolds.

Need to add for genome only mode

@FernandoDuarteF
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The attribute column (9th) is not consistent between gff files. For this reason BUSCO Ideogram fails when using samplesheet.csv as input, but woks with the test dataset.

The busco_create_table_for_plot.R script uses inner_join() to merge both full_table.tsv from BUSCO and the gff annotation into a single table, based on the first field of the attibute column of the gff. However, this field is not consistent among datasets.

Will have to modify the script or look for alternatives.

@FernandoDuarteF
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This is ready to be merged.

@FernandoDuarteF
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It would be good to add a warning that you can't see much on a non chromosome level assembly.

@FernandoDuarteF FernandoDuarteF merged commit a26730a into dev Nov 13, 2024
@FernandoDuarteF FernandoDuarteF deleted the busco_ideograms branch November 13, 2024 09:21
@FernandoDuarteF FernandoDuarteF restored the busco_ideograms branch November 13, 2024 09:30
@chriswyatt1
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#90

Also we should try to solve this too, by printing only chromosomes greater than the size of the smallest that has a busco gene on it... I think that would be the best practise. As we only want to see big chromosomes really, or any with a busco gene

@chriswyatt1 chriswyatt1 deleted the busco_ideograms branch December 2, 2024 09:54
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2 participants